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Secado por atomización de bacterias ácido lácticas: una revisión 乳酸菌的雾化干燥:综述
Pub Date : 2019-06-01 DOI: 10.17230/INGCIENCIA.15.29.7
Madalyd Yurani Vera Peña, Misael Cortés Rodríguez, Francia Elena Valencia-García
Las bacterias ácido lácticas (BAL) son microorganismos que se utilizan como cultivos iniciadores o probióticos, los cuales son de interés en la industria alimentaria y farmacéutica. La preservación de las BAL por diferentes métodos de secado es un tema que ha cobrado interés debido al aumento en la demanda de estos microorganismos. El secado por atomización es un método eficaz que permite obtener productos a bajo costo, con relación al secado por liofilización el cual es comúnmente utilizado para estos microorganismos. El objetivo de esta revisión sistemática es presentar de forma organizada los artículos científicos que han sido publicados en procesos de secado por atomización empleando bacterias ácido lácticas (BAL). La metodología utilizada en la búsqueda y selección de los artículos brinda una exploración durante el periodo 2009- 2019. Los resultados de los diferentes artículos consultados se compararon estableciendo las similitudes metodológicas, se discuten los principales microorganismos estudiados, así como los agentes protectores empleados para mejorar la viabilidad durante el secado por atomización, las condiciones y escala de los equipos de secado por atomización y finalmente las características de los productos secos obtenidos. La información aquí compilada brinda unabase para ajustar la metodología de trabajo cuando se propongan procesos de secado por atomización para BAL.
乳酸细菌(BAL)是一种微生物,用作引发剂或益生菌培养物,在食品和制药工业中具有重要意义。由于对这些微生物的需求增加,通过不同的干燥方法保存球是一个引起人们兴趣的问题。雾化干燥是一种有效的方法,可以以较低的成本获得产品,而冷冻干燥通常用于这些微生物。这一系统审查的目的是有组织地介绍在使用乳酸细菌(BAL)的雾化干燥过程中发表的科学文章。用于搜索和选择文章的方法在2009-2019年期间进行了探索。通过建立方法上的相似性,比较了不同参考文献的结果,讨论了所研究的主要微生物,以及用于提高雾化干燥可行性的保护剂,雾化干燥设备的条件和规模,以及最终获得的干燥产品的特性。这里汇编的信息为在建议BAL雾化干燥过程时调整工作方法提供了依据。
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引用次数: 0
Estimación de los parámetros de dos modelos para la dinámica del dengue y su vector en Cali, Colombia 哥伦比亚卡利登革热动态及其病媒两种模型参数的估计
Pub Date : 2018-12-23 DOI: 10.17230/INGCIENCIA.14.28.3
J. H. Arias, H. Martínez, L. Sepúlveda, Olga Vasilieva
El dengue es una infección viral transmitida por la hembra del mosquito Aedes aegypti que se presenta en todas las regiones tropicales y subtropicales del planeta. En Cali, Colombia, a pesar de los controles que las autoridades de salud dicen estar haciendo, durante el año 2013, se reportaron más de 9.000 casos de dengue, de los cuales algunos han sido graves y otros han llegado a ser letales. Para la transmisión del virus del dengue, los modelos matemáticos que simulan la dinámica de la población infectada, bien sea de humanos, de mosquitos o de ambos, permiten una buena comprensión de la dinámica del virus, por lo que son una excelente herramienta para el seguimiento y control de la enfermedad causada por ellos. Sin embargo, para que esta herramienta sea realmente útil en casos concretos, los modelos deben ser ajustados a las características particulares de la región donde se quieren utilizar. En este artículo, queremos presentar el ajuste de dos modelos matemáticos al área urbana de la ciudad de Cali, Colombia. Inicialmente, con base en el comportamiento natural del mosquito Aedes aegypti en una región como el área de interés, estimamos algunos de los parámetros de los modelos, teniendo en cuenta la literatura existente sobre este tema. Posteriormente, estimamos el resto de parámetros como la solución de mínimos cuadrados que mejor ajusta los resultados de los modelos a los datos de los casos reportados de dengue, según la Secretaria Municipal de Salud de Cali, durante el año 2010.
登革热是一种由雌性埃及伊蚊传播的病毒感染,存在于地球上所有的热带和亚热带地区。在哥伦比亚卡利,尽管卫生当局声称正在进行控制,但2013年报告了9000多例登革热病例,其中一些很严重,另一些已经致命。登革热病毒的病毒传播的数学模型中模拟受感染的人口动态,无论是人类蚊子,或两者兼而有之,允许动态的良好理解病毒,因此是一种优秀的工具,以监测和控制由他们引起的疾病。然而,要使这个工具在特定情况下真正有用,模型必须根据它们想要使用的地区的特殊特征进行调整。在本文中,我们提出了两个数学模型对哥伦比亚卡利市城区的拟合。在本研究中,我们分析了埃及伊蚊在该地区的自然行为,并对模型的一些参数进行了估计,同时考虑了有关该主题的现有文献。然后,根据卡利市卫生部长2010年的数据,我们估计了其余参数作为最小二乘解,使模型结果最符合登革热报告病例的数据。
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引用次数: 4
Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia 哥伦比亚安蒂奥基亚省鉴定了作为甘蔗Pokka-Boheng病病原体的黄腐镰刀菌
Pub Date : 2018-12-23 DOI: 10.17230/INGCIENCIA.14.28.5
Juliana Giraldo-Arias, Santiago Celis-Zapata, Nicolás D. Franco-Sierra, Juan José Arroyave-Toro, Claudia Jaramillo-Mazo, Javier Correa-Álvarez
The cultivation of sugarcane represents an important part in the Colombian economy due to the diverse applications in industries like liquor, food, paper and biofuels. Sugarcane worldwide production is affected by the presence of phytopathogenic agents, mainly filamentous fungi such as Physalospora tucumanensis (red rot disease) and Fusarium spp. To date in Colombia, Pokka boheng disease whose causal agent is the fungus Fusarium verticillioides, has not been reported, which is why it is necessary to identify appropriately this microorganism, responsible for losses in productivity and food contamination. In order to isolate and identify the infectious agent from symptomatic tissues, disinfection and culture in liquid and solid culture mediums were performed in malt extract (2%) and yeast extract (0.2%) both liquid and solid, for 7 days. After several replicates in agar plate, a purification was made along with a morphological characterization based on the shape and color of the mycelium, as well as the type of spores generated. Additionally, the genetic material was extracted and gene markers (ITS, Elongation Factor 1-α (EF) and β-Tubulin (Btub)) were amplified by PCR. Then, DNA sequencing was used to obtain the data to make a phylogenetic reconstruction by probabilistic methods (Maximum Likelihood and Bayesian Inference). The isolated strain, named as EA-FP0013 was located in the Fujikuroi complex group, with high probable identity to Fusarium verticillioides. Thus, early and species-specific identification of these fungal isolates by molecular methods may allow the timely diagnosis of emerging pathophysiological diseases of interest in the region, and thus propose the respective control strategies.
由于甘蔗在酒类、食品、造纸和生物燃料等行业的广泛应用,甘蔗的种植代表了哥伦比亚经济的重要组成部分。世界范围内的甘蔗生产受到植物病原体的影响,主要是丝状真菌,如红腐病和镰刀菌。迄今为止,在哥伦比亚,Pokka boheng病(其病原体是真菌Fusarium verticillioides)尚未报道,这就是为什么有必要适当识别这种导致生产力损失和食品污染的微生物。为了从有症状的组织中分离和鉴定感染原,在液体和固体培养基中分别用麦芽提取物(2%)和酵母提取物(0.2%)进行消毒和培养,培养时间为7 d。在琼脂板上进行多次复制后,进行纯化,并根据菌丝的形状和颜色以及产生的孢子类型进行形态学表征。提取遗传物质,通过PCR扩增ITS、延伸因子1-α (EF)和β-微管蛋白(Btub)等基因标记。然后,利用DNA测序获得数据,通过概率方法(极大似然和贝叶斯推理)进行系统发育重建。该分离株命名为EA-FP0013,属于Fujikuroi复合体群,极有可能为黄斑镰刀菌。因此,通过分子方法对这些真菌分离物进行早期和物种特异性鉴定,可以及时诊断该地区感兴趣的新出现的病理生理疾病,从而提出相应的控制策略。
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引用次数: 1
Implementation of a Non-Invasive Bioprospecting Protocol for Isolation of Lactobacillus from Feces of Hens Under Foraging Conditions 在饲料条件下从母鸡粪便中分离乳酸杆菌的非侵入性生物勘探方案的实施
Pub Date : 2018-12-23 DOI: 10.17230/INGCIENCIA.14.28.4
Simón Robledo-Cardona, S. Ramírez-Hincapié, Javier Correa-Álvarez
In animal production, probiotics seek to replace the use of antibiotics, while diminishing mortality and morbidity rates to raise productivity. Probiotics constitute a natural alternative that, in contrast with antibiotics, neither produces pathogen resistance, nor leaves chemical residues in the final product. Several bacteria, including some belonging to the genus Lactobacillus have been described as probiotics with high potential. A non-invasive bioprospecting protocol aimed for the isolation and characterization of lactobacilli from chicken feces was established. Fecal samples were collected from the ground. These were diluted and cultured in LAB selective medium. Colonies were identified by three methods: Gram stain, MALDI-TOF MS and sequencing of 16S rRNA gene. An initial probiotic potential of lactobacilli isolates was determined via antagonism tests using five enteropathogen reference strains: Staphylococcus aureus, Enterococcus faecium, Candida albicans, Pseudomonas spp. and Salmonella spp. 24 isolates belonging to four Lactobacillus species were identified by MALDITOF MS. BLAST of 16S rRNA gene of eight randomly selected isolates, confirmed MALDI-TOF MS identification. Five of these eight isolates inhibited the growth of at least one of the pathogenic strains used, three isolates of Lactobacillus plantarum and two of Lactobacillus salivarius. Our protocol achieved 21 lactobacilli per 100 isolates performance, greatly surpassing the normal percentage of lactobacilli in chicken gut microbiome, that so, its implementation would facilitate the isolation and identification of new probiotic strains from feces.
在动物生产中,益生菌试图取代抗生素的使用,同时降低死亡率和发病率以提高生产力。益生菌是一种天然的替代品,与抗生素相比,它既不会产生病原体耐药性,也不会在最终产品中留下化学残留物。一些细菌,包括一些属于乳杆菌属的细菌,被描述为具有高潜力的益生菌。建立了一种旨在从鸡粪便中分离和鉴定乳酸杆菌的非侵入性生物勘探方案。粪便样本是从地上采集的。将其稀释并在LAB选择性培养基中培养。菌落鉴定采用三种方法:革兰氏染色法、MALDI-TOF MS法和16S rRNA基因测序法。通过使用五种肠道病原体参考菌株(金黄色葡萄球菌、粪肠球菌、白色念珠菌、假单胞菌和沙门氏菌)的拮抗试验来确定乳酸杆菌分离株的初始益生菌潜力。通过MALDITOF MS鉴定了属于四种乳杆菌的24个分离株。随机选择的8个分离株的16S rRNA基因的BLAST,确认MALDI-TOF MS鉴定。这八个分离株中的五个抑制了至少一种所用致病菌株、三个植物乳杆菌分离株和两个唾液乳杆菌的生长。我们的方案实现了每100个分离株中有21个乳酸杆菌的性能,大大超过了鸡肠道微生物组中乳酸杆菌的正常百分比,因此,它的实施将有助于从粪便中分离和鉴定新的益生菌菌株。
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引用次数: 0
Análisis de las características fisicoquímicas del agua subterránea de la cuenca del río chicú, Colombia, usando indicadores hidroquímicos y estadística multivariante 利用水化学指标和多元统计分析哥伦比亚chicu河流域地下水的理化特性
Pub Date : 2018-12-23 DOI: 10.17230/ingciencia.14.28.2
Cristian Camilo López Velandia
Se evaluaron las características hidroquímicas de una cuenca intramontana, ubicada en la Sabana de Bogotá, usando indicadores y métodos estadísticos, basados en los datos fisicoquímicos de 10 pozos de monitoreo obtenidos durante dos campañas de muestreo realizadas en el año 2014, para determinar las características hidroquímicas generadas por la interacción agua/sedimento del acuífero y determinar las condiciones de calidad del agua subterránea. Para ello, (1) se aplicaron indicadores hidroquímicos para evaluar los procesos de interacción entre el agua y la matriz sedimentaria del acuífero, corroborados mediante (2) la aplicación del análisis de componentes principales, que develó asociaciones fuertes entre las variables (Na+, Mg2+, Ca2+, SO42- y HCO3-), y la influencia de las variables T, pH y STD en los procesos hidroquímicos que gobiernan el acuífero, además (3) se analizaron los parámetros fisicoquímicos, comparándolos con la normatividad ambiental colombiana para consumo humano y para riego. Como resultados, se determinó que en la matriz agua-sedimento, se suscitan procesos de intercambio catiónico reverso y meteorización de los minerales presentes en las formaciones que conforman el acuífero analizado, así mismo, que las muestras de agua obtenidas no presentan características de calidad que permita su uso para riego o consumo humano.
根据2014年进行的两次采样运动中获得的10口监测井的物理化学数据,使用指标和统计方法评估了波哥大大大草原山间盆地的水化学特征,以确定含水层水/沉积物相互作用产生的水化学特征,并确定地下水的质量条件。为此,(1)应用水化学指标来评估水与含水层沉积基质之间的相互作用过程,并通过(2)主成分分析的应用得到了证实,该分析揭示了变量(Na+、Mg2+、Ca2+、SO42-和HCO3-)之间的强相关性,以及变量T、pH和STD对控制含水层的水化学过程的影响,此外(3)分析了物理化学参数,将其与哥伦比亚人类消费和灌溉的环境规范进行比较。结果发现,在水-沉积物基质中,构成所分析含水层的地层中存在的矿物会产生反向阳离子交换和风化过程,此外,获得的水样没有质量特征,无法用于灌溉或人类消费。
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引用次数: 1
Modern Biotechnology for Agricultural Development in Colombia 现代生物技术促进哥伦比亚农业发展
Pub Date : 2018-11-23 DOI: 10.17230/INGCIENCIA.14.28.7
D. Villanueva-Mejía
Colombia is currently one of the most promising countries with regard to its potential for agricultural development and for generating food supply for current and future human generations. This is owing to factors such as availability of land, water, topographical diversity, as well as political factors. Nevertheless, Colombia will reach this full potential if it adopts available technologies that can meet the current global challenges faced by the agriculture in the 21st century: among others, world population growth, increase in average life expectancy, high degree of malnutrition, climate change, wrong agricultural practices. Here is presented how modern biotechnology is an important ally as a wide range of technologies and innovative systems can be applied where they are most needed: for increasing cultivation productivity, resisting both biotic and abiotic factors, and ensuring food safety. In this study is showed evidence with regard to significant benefits of adopting biotechnological crops to contribute to food safety and how they are already being implemented in both developed and developing countries. Using modern technology, there are open opportunities for the country in search of circular bio-based economy, strengthen its food sovereignty and to serve as an agricultural breadbasket to Latin America and the World.
哥伦比亚目前是农业发展潜力和为今世后代提供粮食供应潜力最大的国家之一。这是由于土地、水源、地形多样性以及政治因素等因素造成的。尽管如此,如果哥伦比亚采用能够应对21世纪农业面临的当前全球挑战的现有技术,即世界人口增长、平均预期寿命增加、高度营养不良、气候变化、错误的农业做法,它将充分发挥这一潜力。本文介绍了现代生物技术如何成为一个重要的盟友,因为各种技术和创新系统可以应用于最需要的地方:提高种植生产力,抵御生物和非生物因素,并确保食品安全。在这项研究中,有证据表明采用生物技术作物有助于食品安全的重大好处,以及发达国家和发展中国家是如何实施这些好处的。利用现代技术,该国有机会寻求循环生物经济,加强其粮食主权,并成为拉丁美洲和世界的农业粮仓。
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引用次数: 2
Remoción de contaminantes y crecimiento del alga Scenedesmus sp. en aguas residuales de curtiembres, comparación entre células libres e inmovilizadas 制革废水中Scenedesmus sp.藻类的去除和生长,游离细胞和固定细胞的比较
Pub Date : 2018-11-23 DOI: 10.17230/INGCIENCIA.14.28.1
A. G. Rosales, C. D. Rodríguez, M. Ballén-Segura
Las aguas residuales de curtiembres conllevan un problema ambiental y de salud, debido a las altas concentraciones de contaminantes como el cromo, sulfuros, entre otros. Una forma de realizar el tratamiento de estas aguas es por medio del uso de microorganismos tales como las microalgas, debido a su capacidad de utilizar los compuestos inorgánicos y acumular metales y otras sustancias contaminantes. Esta capacidad podría verse magnificada con la inmovilización celular en diferentes matrices. En este artículo se presenta una evaluación de la capacidad de crecimiento y de remoción de nitrógeno, fósforo y cromo por parte de la microalga Scenedesmus sp., comparando la eficiencia entre las células libres e inmovilizadas en una matriz de esponja de Luffa. De esta manera, se obtuvo un mayor crecimiento en las células libres en comparación a las inmovilizadas, aunque sin diferencias significativas entre ambos experimentos. En ambos casos se observaron remociones superiores al 90% para NO–3 , NH+4, PO34 y cromo total, así como una reducción importante de la DBO y DQO (> 97 %) que puede deberse a efecto sinérgico con las bacterias presentes en los cultivos de la microalga. Finalmente, los porcentajes de lípidos en ambos casos fueron superiores al 20% indicando que esta biomasa podría presentar un alto potencial como materia prima para la generación de biodiesel.
由于铬、硫化物等污染物浓度高,制革废水带来了环境和健康问题。处理这些水的一种方法是使用微生物,如微藻,因为它们有能力使用无机化合物并积累金属和其他污染物质。这种能力可以通过在不同基质中固定细胞来放大。本文通过比较游离细胞和固定在丝瓜海绵基质中的细胞之间的效率,评估了微藻Scenedesmus sp的生长能力以及对氮、磷和铬的去除能力。通过这种方式,与固定化细胞相比,自由细胞获得了更大的生长,尽管两个实验之间没有显着差异。在这两种情况下,都观察到NO-3、NH+4、PO34和总铬的去除率超过90%,BOD和COD(>97%)显著降低,这可能是由于与微藻培养中存在的细菌协同作用。最后,这两种情况下的脂质百分比都超过20%,这表明这种生物量可能具有很高的潜力,可以作为生产生物柴油的原料。
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引用次数: 10
Tampering Detection on Digital Evidence for Forensics Purposes 用于取证目的的数字证据篡改检测
Pub Date : 2018-06-14 DOI: 10.17230/INGCIENCIA.13.27.3
H. D. Ortiz, R. Diego, L. DoraM.Ballesteros
In terms of digital evidence, images of a crime scene play an important role in a legal process; therefore it is essential to guarantee their chain of custody. If the image is tampered with operations such as cropping, duplicity or blurring of objects, there must be a mechanism that identifies the existence of such manipulation. In this paper, a solution to the chain of custody based on a technique known as watermarking is proposed. In order to validate the sensitivity and effectiveness of the proposed system, six classes of manipulations are applied to watermarked images; finding a high sensitivity to small manipulations (e.g 0.25% of the image size), since in all cases the manipulation was identified. With the proposed solution, legal authority can rely on the chain of custody of the digital evidence.
就数字证据而言,犯罪现场的图像在法律程序中发挥着重要作用;因此,保证他们的监管链是至关重要的。如果图像被剪切、复制或模糊对象等操作篡改,则必须有一种机制来识别这种操作的存在。本文提出了一种基于水印技术的保管链解决方案。为了验证所提出的系统的灵敏度和有效性,将六类操作应用于水印图像;发现对小操作(例如图像大小的0.25%)的高灵敏度,因为在所有情况下操作都被识别。有了拟议的解决方案,法律权威机构可以依赖数字证据的保管链。
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引用次数: 2
Risk Assessment of Shallow Landslides for Infrastructure Projects: Analysis of the Case of a Landslide in the El Cabuyal Zone 基础设施项目浅层滑坡风险评估——以El cabual地区滑坡为例分析
Pub Date : 2018-06-14 DOI: 10.17230/INGCIENCIA.13.27.7
R. Marín, J. C. Guzmán-Martínez, H. Carvajal, E. García-Aristizabal, Juan Diego Cadavid-Arango, Paulina Agudelo-Vallejo
In this paper an assessment of the hazard, the vulnerability and the risk associated with shallow landslides in a watershed of the Valle de Aburra is carried out. The TRIGRS model with a Monte Carlo simulation to estimate the probability of failure in the watershed using water table depth as the trigger factor is implemented, which allows the assessment of the hazard along with its annual probability of occurrence. Additionally, a quantitative model for physical vulnerability of structures which takes into account landslide intensity and resistance of exposed elements is used. The annual risk is estimated for a house of the study site as the product of the hazard and the damage costs due to the occurrence of the catastrophic event, which considers vulnerability. The methodology used focuses on the shallow landslide occurred on October 26, 2016 on a slope within the study site, which affected the house that is being analyzed.
本文对Aburra流域浅层滑坡的危害、脆弱性和风险进行了评估。利用地下水位深度作为触发因子,利用蒙特卡罗模拟估算流域失败概率的TRIGRS模型实现了灾害及其年发生概率的评估。此外,还建立了考虑滑坡强度和暴露单元阻力的结构物易损性定量模型。在考虑脆弱性的情况下,将研究地点房屋的年风险估计为灾害与发生灾难性事件造成的损失成本的乘积。所使用的方法侧重于2016年10月26日在研究地点的一个斜坡上发生的浅层滑坡,该滑坡影响了正在分析的房屋。
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引用次数: 1
Leave-one-out evaluation of the nearest feature line and the rectified nearest feature line segment classifiers using Multi-core architectures 使用多核架构对最近的特征线和校正的最近的特征线段分类器进行留一评估
Pub Date : 2018-06-01 DOI: 10.17230/INGCIENCIA.14.27.4
Ana-Lorena Uribe-Hurtado, M. Orozco-Alzate, Eduardo-José Villegas-Jaramillo
In this paper we present the parallelization of the leave-one-out test: areproducible test that is, in general, computationally expensive. Paral-lelization was implemented on multi-core multi-threaded architectures, us-ing the Flynn Single Instruction Multiple Data taxonomy. This techniquewas used for the preprocessing and processing stages of two classificationalgorithms that are oriented to enrich the representation in small samplecases: the nearest feature line (NFL) algorithm and the rectified nearestfeature line segment (RNFLS) algorithm. Results show an accelerationof up to 18.17 times with the smallest dataset and 29.91 times with thelargest one, using the most costly algorithm (RNFLS) whose complexityisO(n4). The paper also shows the pseudo-codes of the serial and parallel algorithms using, in the latter case, a notation that describes the way theparallelization was carried out as a function of the threads.
在本文中,我们提出了“留一”测试的并行化:一个可生产的测试,通常计算成本很高。并行化是在多核多线程体系结构上实现的,使用Flynn单指令多数据分类法。该技术用于两种分类算法的预处理和处理阶段,这两种算法旨在丰富小样本情况下的表示:最近特征线(NFL)算法和校正最近特征线段(RNFLS)算法。结果显示,使用复杂度为O(n4)的最昂贵算法(RNFLS),最小数据集的加速率高达18.17倍,最大数据集的加快率高达29.91倍。本文还展示了串行和并行算法的伪代码,在后一种情况下,使用一种符号来描述并行化作为线程函数的执行方式。
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引用次数: 1
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Ingenieria y Ciencia
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