Traditional techniques to evaluate the aggregation of red blood cells by optical methods require large sample volume and provide parameters that vary significantly from one method to another. A simplified variant of a chip system previously developed by Shin et al. (2009)1 based on light transmission for measuring erythrocyte aggregation is presented. Through a detailed analysis of intensity versus time curves, relevant information about erythrocyte aggregation and its variables is obtained. Parameters that provide more accuracy for the diagnosis of patients in order to have an immediate application in Clinical Medicine are proposed.
{"title":"Simplified variant of an optical chip to evaluate aggregation of red blood cells","authors":"M. Toderi, B. Riquelme, H. Castellini","doi":"10.1117/12.2180907","DOIUrl":"https://doi.org/10.1117/12.2180907","url":null,"abstract":"Traditional techniques to evaluate the aggregation of red blood cells by optical methods require large sample volume and provide parameters that vary significantly from one method to another. A simplified variant of a chip system previously developed by Shin et al. (2009)1 based on light transmission for measuring erythrocyte aggregation is presented. Through a detailed analysis of intensity versus time curves, relevant information about erythrocyte aggregation and its variables is obtained. Parameters that provide more accuracy for the diagnosis of patients in order to have an immediate application in Clinical Medicine are proposed.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"536 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134090464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Dolinko, C. Valencia, D. Skigin, M. Inchaussandague, Analía Tolivia, V. Conforti
Euglenoids are a group of predominantly free-living unicellular microorganisms that mostly live in freshwater bodies but can also be found in marine and brackish waters. These organisms have a characteristic that distinguishes them form the other protists: they are covered by a surface pellicle formed by S-shaped overlapping bands which resemble a diffraction grating. These microorganisms have developed numerous protection mechanisms intended to avoid or reduce the damage produced by UV radiation, such as the production of pigments and the repair mechanisms in hours of darkness and during daylight. In a recent paper we have investigated the role played by the pellicle of Euglenoids in the protection of the cell against UV radiation, by means of an electromagnetic approach based on the approximation of the pellicle profile by a one-dimensional diffraction grating. This simplified model allowed us to confirm that under certain incidence conditions, the corrugation of the pellicle helps increase the UV reflection, and consequently, diminish the UV radiation that enters the cell. In order to analyze the electromagnetic response of the whole cell, we extend two different approaches to calculate the reflected response: a simulation method especially developed to deal with complex biological structures that permits the introduction of the scattering object via an electron microscopy image, and the integral method, which has been widely used to compute the electromagnetic response of finite structures. Numerical results of near and far fields are shown.
{"title":"UV protection of euglenoids: computation of the electromagnetic response","authors":"A. Dolinko, C. Valencia, D. Skigin, M. Inchaussandague, Analía Tolivia, V. Conforti","doi":"10.1117/12.2180935","DOIUrl":"https://doi.org/10.1117/12.2180935","url":null,"abstract":"Euglenoids are a group of predominantly free-living unicellular microorganisms that mostly live in freshwater bodies but can also be found in marine and brackish waters. These organisms have a characteristic that distinguishes them form the other protists: they are covered by a surface pellicle formed by S-shaped overlapping bands which resemble a diffraction grating. These microorganisms have developed numerous protection mechanisms intended to avoid or reduce the damage produced by UV radiation, such as the production of pigments and the repair mechanisms in hours of darkness and during daylight. In a recent paper we have investigated the role played by the pellicle of Euglenoids in the protection of the cell against UV radiation, by means of an electromagnetic approach based on the approximation of the pellicle profile by a one-dimensional diffraction grating. This simplified model allowed us to confirm that under certain incidence conditions, the corrugation of the pellicle helps increase the UV reflection, and consequently, diminish the UV radiation that enters the cell. In order to analyze the electromagnetic response of the whole cell, we extend two different approaches to calculate the reflected response: a simulation method especially developed to deal with complex biological structures that permits the introduction of the scattering object via an electron microscopy image, and the integral method, which has been widely used to compute the electromagnetic response of finite structures. Numerical results of near and far fields are shown.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132328215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Quinto, C. B. Zamataro, C. Benetti, D. A. Dias, A. Blay, D. Zezell
Knowledge of dental structures is essential for understanding of laser interaction and its consequences during adhesion processes. Tubule density in dentin ranges from 4.900 to 90.000 per mm2, for diameters from 1 to 3 μm. Light propagation inside the tubules is associated with tubules orientation. To the best of our knowledge, there is no previous work in literature characterizing physical-chemical alterations in dentin. The dentin samples were irradiated with a Er,Cr:YSGG Laser at wavelength 2.78 μm, with an energy density of 9.46 J/cm2 , above the ablation threshold. ATRFTIR at wavenumbers 2000 to 700 cm-1 was used to evaluate the differences among third root region and tubules orientation.
{"title":"Optical properties of human radicular dentin: ATR-FTIR characterization and dentine tubule direction influence on radicular post adhesion","authors":"J. Quinto, C. B. Zamataro, C. Benetti, D. A. Dias, A. Blay, D. Zezell","doi":"10.1117/12.2181162","DOIUrl":"https://doi.org/10.1117/12.2181162","url":null,"abstract":"Knowledge of dental structures is essential for understanding of laser interaction and its consequences during adhesion processes. Tubule density in dentin ranges from 4.900 to 90.000 per mm2, for diameters from 1 to 3 μm. Light propagation inside the tubules is associated with tubules orientation. To the best of our knowledge, there is no previous work in literature characterizing physical-chemical alterations in dentin. The dentin samples were irradiated with a Er,Cr:YSGG Laser at wavelength 2.78 μm, with an energy density of 9.46 J/cm2 , above the ablation threshold. ATRFTIR at wavenumbers 2000 to 700 cm-1 was used to evaluate the differences among third root region and tubules orientation.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122257796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. M. Silva, L. Alencar, M. Bernardi, F. A. S. Lima, C. Melo
In this work we investigate the scattering of light in means turbid in the presence or not of pigment and nanoparticles. For this we initially using a sample of collagen from means turbid with and without the presence of curcuma pigments and nanoparticles. Our results show that the light scattering is more intense in the samples with nanoparticles and curcuma pigment.
{"title":"Study of the scattering of the light in aqueous samples collagen in the presence of nanoparticles and curcuma pigment","authors":"F. M. Silva, L. Alencar, M. Bernardi, F. A. S. Lima, C. Melo","doi":"10.1117/12.2181121","DOIUrl":"https://doi.org/10.1117/12.2181121","url":null,"abstract":"In this work we investigate the scattering of light in means turbid in the presence or not of pigment and nanoparticles. For this we initially using a sample of collagen from means turbid with and without the presence of curcuma pigments and nanoparticles. Our results show that the light scattering is more intense in the samples with nanoparticles and curcuma pigment.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"187 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121193064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Sadraeian, F. M. Tsutae, H. H. Moreira, A. P. Araujo, F. G. Guimarães, S. Pincus
Pulchellin is a type 2 of ribosome-inactivating proteins isolated from some seeds significantly growing in Brazil. It is a potent agent to inhibit the protein synthesis in cancer cells and also HIV-infected cells. Pulchellin can be conjugated to HIV monoclonal antibodies to specifically target the HIV-infected cells. To analyze the protein synthesis inhibition by Pulchellin, the intracellular localization of the immunoconjugate should be compared to Pulchellin. In this case, the intracellular trafficking of this protein in cells can be determined by confocal microscopy. In our study, we utilized Pulchellin to construct HIV monoclonal antibody-conjugated Pulchellin A chain in order to target HIV-infected lymphocyte cells. Afterward the conjugation was labeled with the superior Alexa Fluor 488 dye. As a subsequent step, we are interested in studying the intracellular trafficking pathway of this novel conjugation in HIV-infected cells by confocal microscopy. Moreover, possible quantitative methods for fluorescent labeling of the immunoconjugate during confocal microscopy will be investigated.
{"title":"Preparation of HIV monoclonal antibody-conjugated pulchellin in order to study its intracellular trafficking pathway in HIV-infected cells by confocal microscopy","authors":"Mohammad Sadraeian, F. M. Tsutae, H. H. Moreira, A. P. Araujo, F. G. Guimarães, S. Pincus","doi":"10.1117/12.2181011","DOIUrl":"https://doi.org/10.1117/12.2181011","url":null,"abstract":"Pulchellin is a type 2 of ribosome-inactivating proteins isolated from some seeds significantly growing in Brazil. It is a potent agent to inhibit the protein synthesis in cancer cells and also HIV-infected cells. Pulchellin can be conjugated to HIV monoclonal antibodies to specifically target the HIV-infected cells. To analyze the protein synthesis inhibition by Pulchellin, the intracellular localization of the immunoconjugate should be compared to Pulchellin. In this case, the intracellular trafficking of this protein in cells can be determined by confocal microscopy. In our study, we utilized Pulchellin to construct HIV monoclonal antibody-conjugated Pulchellin A chain in order to target HIV-infected lymphocyte cells. Afterward the conjugation was labeled with the superior Alexa Fluor 488 dye. As a subsequent step, we are interested in studying the intracellular trafficking pathway of this novel conjugation in HIV-infected cells by confocal microscopy. Moreover, possible quantitative methods for fluorescent labeling of the immunoconjugate during confocal microscopy will be investigated.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121409148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. A. Gómez-García, A. Arranz, M. Fresno, M. Desco, U. Mahmood, J. Vaquero, J. Ripoll
Endoscopy is frequently used in the diagnosis of several gastro-intestinal pathologies as Crohn disease, ulcerative colitis or colorectal cancer. It has great potential as a non-invasive screening technique capable of detecting suspicious alterations in the intestinal mucosa, such as inflammatory processes. However, these early lesions usually cannot be detected with conventional endoscopes, due to lack of cellular detail and the absence of specific markers. Due to this lack of specificity, the development of new endoscopy technologies, which are able to show microscopic changes in the mucosa structure, are necessary. We here present a confocal endomicroscope, which in combination with a wide field fluorescence endoscope offers fast and specific macroscopic information through the use of activatable probes and a detailed analysis at cellular level of the possible altered tissue areas. This multi-modal and multi-scale imaging module, compatible with commercial endoscopes, combines near-infrared fluorescence (NIRF) measurements (enabling specific imaging of markers of disease and prognosis) and confocal endomicroscopy making use of a fiber bundle, providing a cellular level resolution. The system will be used in animal models exhibiting gastro-intestinal diseases in order to analyze the use of potential diagnostic markers in colorectal cancer. In this work, we present in detail the set-up design and the software implementation in order to obtain simultaneous RGB/NIRF measurements and short confocal scanning times.
{"title":"Fluorescence multi-scale endoscopy and its applications in the study and diagnosis of gastro-intestinal diseases: set-up design and software implementation","authors":"P. A. Gómez-García, A. Arranz, M. Fresno, M. Desco, U. Mahmood, J. Vaquero, J. Ripoll","doi":"10.1117/12.2189960","DOIUrl":"https://doi.org/10.1117/12.2189960","url":null,"abstract":"Endoscopy is frequently used in the diagnosis of several gastro-intestinal pathologies as Crohn disease, ulcerative colitis or colorectal cancer. It has great potential as a non-invasive screening technique capable of detecting suspicious alterations in the intestinal mucosa, such as inflammatory processes. However, these early lesions usually cannot be detected with conventional endoscopes, due to lack of cellular detail and the absence of specific markers. Due to this lack of specificity, the development of new endoscopy technologies, which are able to show microscopic changes in the mucosa structure, are necessary. We here present a confocal endomicroscope, which in combination with a wide field fluorescence endoscope offers fast and specific macroscopic information through the use of activatable probes and a detailed analysis at cellular level of the possible altered tissue areas. This multi-modal and multi-scale imaging module, compatible with commercial endoscopes, combines near-infrared fluorescence (NIRF) measurements (enabling specific imaging of markers of disease and prognosis) and confocal endomicroscopy making use of a fiber bundle, providing a cellular level resolution. The system will be used in animal models exhibiting gastro-intestinal diseases in order to analyze the use of potential diagnostic markers in colorectal cancer. In this work, we present in detail the set-up design and the software implementation in order to obtain simultaneous RGB/NIRF measurements and short confocal scanning times.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"117 44","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120826126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camila de Paula D´Almeida, C. Campos, Marcelo Saito Nogueira, S. Pratavieira, C. Kurachi
pathology. The optical properties of these intrinsic fluorophores respond to the microenvironment and the metabolic status, thus making fluorescence spectroscopy a valuable tool to study the conditions of biological tissues. The purpose of this study is to investigate the hairless mice skin metabolic changes during the photoaging process through lifetime and fluorescence measurements targeting NADH and FAD. Two lasers centered at 378 nm and 445 nm, respectively, perform excitation of NADH and FAD. The fluorescence acquisition is carried out at mice dorsal and ventral regions throughout the photoaging protocol and aging process. Differences in fluorescence and lifetime data between young and photoaged mice measurements were observed. The endogenous fluorescence spectrum of photoaged dorsal skin showed an increase compared to young and aged skin. Lifetime of bound NADH and free FAD presented an increase in the first week that continued until the end of the protocol. Aging process is being investigated to complement the information obtained from fluorescence data and lifetime of photoaging process.
{"title":"Time-resolved and steady-state fluorescence spectroscopy for the assessment of skin photoaging process","authors":"Camila de Paula D´Almeida, C. Campos, Marcelo Saito Nogueira, S. Pratavieira, C. Kurachi","doi":"10.1117/12.2180975","DOIUrl":"https://doi.org/10.1117/12.2180975","url":null,"abstract":"pathology. The optical properties of these intrinsic fluorophores respond to the microenvironment and the metabolic status, thus making fluorescence spectroscopy a valuable tool to study the conditions of biological tissues. The purpose of this study is to investigate the hairless mice skin metabolic changes during the photoaging process through lifetime and fluorescence measurements targeting NADH and FAD. Two lasers centered at 378 nm and 445 nm, respectively, perform excitation of NADH and FAD. The fluorescence acquisition is carried out at mice dorsal and ventral regions throughout the photoaging protocol and aging process. Differences in fluorescence and lifetime data between young and photoaged mice measurements were observed. The endogenous fluorescence spectrum of photoaged dorsal skin showed an increase compared to young and aged skin. Lifetime of bound NADH and free FAD presented an increase in the first week that continued until the end of the protocol. Aging process is being investigated to complement the information obtained from fluorescence data and lifetime of photoaging process.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124028567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Soares, Sidnei Nahórny, F. R. Marciano, H. Zanin, A. Lobo
New biomaterials such as multi-walled carbon nanotubes oxide/graphene oxide (MWCNTO/GO), nanohydroxyapatite (nHAp) and combination of them together or not to acidulated phosphate fluoride gel (F) have been tested as protective coating before root dentin erosion. Fourteen bovine teeth were cleaned, polished, divided into two parts (n=28) and assigned to seven groups: (Control) – without previous surface treatment; F treatment; nHAp; MWCNTO/GO; F+nHAp; F+MWCNTO/GO and F+MWCNTO/GO/nHAp composites. Each sample had two sites of pre-treatments: acid etched area and an area without treatment. After the biomaterials application, the samples were submitted to six cycles (demineralization: orange juice, 10 min; remineralization: artificial saliva, 1 h). Micro energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) mapping area analyses were performed after erosive cycling on both sites (n=84). μ-EDXRF mappings showed that artificial saliva and MWCNTO/GO/nHAp/F composite treatments produced lower dentin demineralization than in the other groups. Exposed dentin tubules allowed better interaction of nanobiomaterials than in smear layer covered dentin. Association of fluoride with other biomaterials had a positive influence on acid etched dentin. MWCNTO/GO/nHAp/F composite treatment resulted in levels of demineralization similar to the control group.
{"title":"Micro energy-dispersive x-ray fluorescence spectrometry study of dentin coating with nanobiomaterials","authors":"L. Soares, Sidnei Nahórny, F. R. Marciano, H. Zanin, A. Lobo","doi":"10.1117/12.2180965","DOIUrl":"https://doi.org/10.1117/12.2180965","url":null,"abstract":"New biomaterials such as multi-walled carbon nanotubes oxide/graphene oxide (MWCNTO/GO), nanohydroxyapatite (nHAp) and combination of them together or not to acidulated phosphate fluoride gel (F) have been tested as protective coating before root dentin erosion. Fourteen bovine teeth were cleaned, polished, divided into two parts (n=28) and assigned to seven groups: (Control) – without previous surface treatment; F treatment; nHAp; MWCNTO/GO; F+nHAp; F+MWCNTO/GO and F+MWCNTO/GO/nHAp composites. Each sample had two sites of pre-treatments: acid etched area and an area without treatment. After the biomaterials application, the samples were submitted to six cycles (demineralization: orange juice, 10 min; remineralization: artificial saliva, 1 h). Micro energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) mapping area analyses were performed after erosive cycling on both sites (n=84). μ-EDXRF mappings showed that artificial saliva and MWCNTO/GO/nHAp/F composite treatments produced lower dentin demineralization than in the other groups. Exposed dentin tubules allowed better interaction of nanobiomaterials than in smear layer covered dentin. Association of fluoride with other biomaterials had a positive influence on acid etched dentin. MWCNTO/GO/nHAp/F composite treatment resulted in levels of demineralization similar to the control group.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"9531 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129741646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. C. Mota, Bruna A. Guerra, Brena S. A. Machado, A. J. Cabral, A. Gomes
Resin composites are widely used as restorative materials due to their excellent aesthetical and mechanical properties. Posterior teeth are constantly submitted to occlusal stress and upon restoration require more resistant resins. The aim of this study was to analyze in vitro the wear suffered over time by restorations in resin composite in posterior teeth, by Optical Coherence Tomography (OCT). 30 molars had occlusal cavities prepared and were randomly divided into three groups (n=10) and restored with resin composite: G1: Filtek P90 (3M/ESPE), G2: Tetric N-Ceram (Ivoclar Vivadent); G3: Filtek P60 (3M/ESPE). Specimens were subjected to initial analysis by OCT (OCP930SR, Thorlabs, axial resolution 6.2 μm) and stereoscopic microscope. Specimens were submitted to thermocycling (500 cycles, 5-55 °C) and subjected to simulated wear through a machine chewing movements (Wear Machine WM001), projecting four years of use. After mechanical cycles, the specimens were submitted to a second evaluation by the OCT and stereoscopic microscopy. As a result, it was observed that 90% of the restorations of both groups had fractures and/or points of stress concentration, considered niches for early dissemination of new fracture lines. It was also found that G1 and G2 had more points of stress concentration, whereas G3 had a higher incidence of fracture lines already propagated. It was concluded that the G3 showed more brittle behavior at the masticatory wear when compared to G1 and G2.
{"title":"Optical coherence tomography applied to the evaluation of wear of composite resin for posterior teeth","authors":"C. C. Mota, Bruna A. Guerra, Brena S. A. Machado, A. J. Cabral, A. Gomes","doi":"10.1117/12.2180753","DOIUrl":"https://doi.org/10.1117/12.2180753","url":null,"abstract":"Resin composites are widely used as restorative materials due to their excellent aesthetical and mechanical properties. Posterior teeth are constantly submitted to occlusal stress and upon restoration require more resistant resins. The aim of this study was to analyze in vitro the wear suffered over time by restorations in resin composite in posterior teeth, by Optical Coherence Tomography (OCT). 30 molars had occlusal cavities prepared and were randomly divided into three groups (n=10) and restored with resin composite: G1: Filtek P90 (3M/ESPE), G2: Tetric N-Ceram (Ivoclar Vivadent); G3: Filtek P60 (3M/ESPE). Specimens were subjected to initial analysis by OCT (OCP930SR, Thorlabs, axial resolution 6.2 μm) and stereoscopic microscope. Specimens were submitted to thermocycling (500 cycles, 5-55 °C) and subjected to simulated wear through a machine chewing movements (Wear Machine WM001), projecting four years of use. After mechanical cycles, the specimens were submitted to a second evaluation by the OCT and stereoscopic microscopy. As a result, it was observed that 90% of the restorations of both groups had fractures and/or points of stress concentration, considered niches for early dissemination of new fracture lines. It was also found that G1 and G2 had more points of stress concentration, whereas G3 had a higher incidence of fracture lines already propagated. It was concluded that the G3 showed more brittle behavior at the masticatory wear when compared to G1 and G2.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130751059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The pharyngitis and laryngitis are respiratory tract infections highly common. Pharyngitis can be accompanied by fever, especially if caused by a systemic infection. Laryngitis is an inflammation of your voice box (larynx) from irritation or infection. The conventional treatment is the antibiotics administration, which may be responsible by an increase of identification of bacterial strains resistant to drug. This fact associated to high incidence of these infections become important to develop new technologies for diagnosis. This study aims to evaluate the use of widefield fluorescence imaging for the characterization of oropharynx infections, in order to diagnose the bacteria colonization. The imaging system for wide field fluorescence visualization is Evince® (MMOptics, São Carlos, SP, Brazil) coupled to an Apple iPhone® cell phone device. The system consists of Light Emitting Diodes (LEDs) operating in the violet blue region centered at green-red spectrum 450 nm and optical filters that allow viewing of fluorescence. A tongue depressor was adapted to Evince® for mouth opening. The same images were captured with white light and fluorescence with an optical system. The red fluorescence may be a bacterial marker for physiological monitoring of oropharynx infection processes. The bacterial biofilm on tissue were assigned to the presence of protoporphyrin IX. This work indicates that the autofluorescence of the tissue may be used as a non-invasive technique to aid in the oropharynx infection diagnostic.
咽炎和喉炎是非常常见的呼吸道感染。咽炎可伴有发烧，特别是由全身感染引起的咽炎。喉炎是由刺激或感染引起的喉部炎症。传统的治疗方法是使用抗生素，这可能是由于增加了对药物耐药菌株的鉴定。与这些感染的高发病率相关的这一事实对于开发新的诊断技术非常重要。本研究旨在评价宽视场荧光成像在口咽部感染表征中的应用，以诊断细菌定植。用于宽视场荧光可视化的成像系统是Evince®(MMOptics, s o Carlos, SP, Brazil)与Apple iPhone®手机设备耦合。该系统由发光二极管(led)组成，工作在以450纳米绿红色光谱为中心的紫蓝色区域，以及允许观察荧光的光学滤光片。Evince®采用了压舌板进行开口。同样的图像是用光学系统用白光和荧光捕获的。红色荧光可能是生理监测口咽部感染过程的细菌标志物。组织上的细菌生物膜被分配到原卟啉IX的存在。这项工作表明，组织的自身荧光可以作为一种非侵入性技术来帮助口咽感染的诊断。
{"title":"Fluorescence diagnosis of upper respiratory tract infections","authors":"K. Blanco, N. Inada, C. Kurachi, V. Bagnato","doi":"10.1117/12.2180945","DOIUrl":"https://doi.org/10.1117/12.2180945","url":null,"abstract":"The pharyngitis and laryngitis are respiratory tract infections highly common. Pharyngitis can be accompanied by fever, especially if caused by a systemic infection. Laryngitis is an inflammation of your voice box (larynx) from irritation or infection. The conventional treatment is the antibiotics administration, which may be responsible by an increase of identification of bacterial strains resistant to drug. This fact associated to high incidence of these infections become important to develop new technologies for diagnosis. This study aims to evaluate the use of widefield fluorescence imaging for the characterization of oropharynx infections, in order to diagnose the bacteria colonization. The imaging system for wide field fluorescence visualization is Evince® (MMOptics, São Carlos, SP, Brazil) coupled to an Apple iPhone® cell phone device. The system consists of Light Emitting Diodes (LEDs) operating in the violet blue region centered at green-red spectrum 450 nm and optical filters that allow viewing of fluorescence. A tongue depressor was adapted to Evince® for mouth opening. The same images were captured with white light and fluorescence with an optical system. The red fluorescence may be a bacterial marker for physiological monitoring of oropharynx infection processes. The bacterial biofilm on tissue were assigned to the presence of protoporphyrin IX. This work indicates that the autofluorescence of the tissue may be used as a non-invasive technique to aid in the oropharynx infection diagnostic.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131024166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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