C. R. Silva, C. M. Camargo, D. P. Aureliano, L. D. De Pretto, A. Z. Freitas, M. Ribeiro
Optical properties of the biological tissue play an important role to a correct use of optical techniques for therapy and diagnosis. The mice skin presents morphological differences due to characteristics such as gender, body mass and age. Murine models are frequently used in pre-clinical trials in optical therapy and diagnosis. Therefore, the assessment of the skin tissue in animal models is needed for a proper understanding of how light interacts with skin. Noninvasive techniques such as optical coherence tomography (OCT) have been used to obtain optical information of the tissue, as the attenuation coefficient, with the advantage of obtaining sectional images in real time. In this study, eight female BALB/c albino mice (twenty-four weeks old) and eight male C57BL/6 black mice (eight weeks old) were used to measure the attenuation coefficient of the light in the skin, utilizing the OCT technique, aiming to check for influence of the aging process. Two moments were assessed twenty-two weeks apart from each other. Our data show that the aging process significantly affects the light attenuation coefficient in mice skin. Twenty-two weeks after, statistical significant differences were observed between groups within a same strain. We conclude that light attenuation coefficient of mice skin may be influenced by factors such as disorganization of the dermis. Morphological aspects of skin should be taken into account in studies that involve optical strategies in murine models.
{"title":"Attenuation coefficient of the light in skin of BALB/c and C57BL/6 mice","authors":"C. R. Silva, C. M. Camargo, D. P. Aureliano, L. D. De Pretto, A. Z. Freitas, M. Ribeiro","doi":"10.1117/12.2181015","DOIUrl":"https://doi.org/10.1117/12.2181015","url":null,"abstract":"Optical properties of the biological tissue play an important role to a correct use of optical techniques for therapy and diagnosis. The mice skin presents morphological differences due to characteristics such as gender, body mass and age. Murine models are frequently used in pre-clinical trials in optical therapy and diagnosis. Therefore, the assessment of the skin tissue in animal models is needed for a proper understanding of how light interacts with skin. Noninvasive techniques such as optical coherence tomography (OCT) have been used to obtain optical information of the tissue, as the attenuation coefficient, with the advantage of obtaining sectional images in real time. In this study, eight female BALB/c albino mice (twenty-four weeks old) and eight male C57BL/6 black mice (eight weeks old) were used to measure the attenuation coefficient of the light in the skin, utilizing the OCT technique, aiming to check for influence of the aging process. Two moments were assessed twenty-two weeks apart from each other. Our data show that the aging process significantly affects the light attenuation coefficient in mice skin. Twenty-two weeks after, statistical significant differences were observed between groups within a same strain. We conclude that light attenuation coefficient of mice skin may be influenced by factors such as disorganization of the dermis. Morphological aspects of skin should be taken into account in studies that involve optical strategies in murine models.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122646900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Soares, Sidnei Nahórny, F. R. Marciano, H. Zanin, A. Lobo
New biomaterials such as multi-walled carbon nanotubes oxide/graphene oxide (MWCNTO/GO), nanohydroxyapatite (nHAp) and combination of them together or not to acidulated phosphate fluoride gel (F) have been tested as protective coating before root dentin erosion. Fourteen bovine teeth were cleaned, polished, divided into two parts (n=28) and assigned to seven groups: (Control) – without previous surface treatment; F treatment; nHAp; MWCNTO/GO; F+nHAp; F+MWCNTO/GO and F+MWCNTO/GO/nHAp composites. Each sample had two sites of pre-treatments: acid etched area and an area without treatment. After the biomaterials application, the samples were submitted to six cycles (demineralization: orange juice, 10 min; remineralization: artificial saliva, 1 h). Micro energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) mapping area analyses were performed after erosive cycling on both sites (n=84). μ-EDXRF mappings showed that artificial saliva and MWCNTO/GO/nHAp/F composite treatments produced lower dentin demineralization than in the other groups. Exposed dentin tubules allowed better interaction of nanobiomaterials than in smear layer covered dentin. Association of fluoride with other biomaterials had a positive influence on acid etched dentin. MWCNTO/GO/nHAp/F composite treatment resulted in levels of demineralization similar to the control group.
{"title":"Micro energy-dispersive x-ray fluorescence spectrometry study of dentin coating with nanobiomaterials","authors":"L. Soares, Sidnei Nahórny, F. R. Marciano, H. Zanin, A. Lobo","doi":"10.1117/12.2180965","DOIUrl":"https://doi.org/10.1117/12.2180965","url":null,"abstract":"New biomaterials such as multi-walled carbon nanotubes oxide/graphene oxide (MWCNTO/GO), nanohydroxyapatite (nHAp) and combination of them together or not to acidulated phosphate fluoride gel (F) have been tested as protective coating before root dentin erosion. Fourteen bovine teeth were cleaned, polished, divided into two parts (n=28) and assigned to seven groups: (Control) – without previous surface treatment; F treatment; nHAp; MWCNTO/GO; F+nHAp; F+MWCNTO/GO and F+MWCNTO/GO/nHAp composites. Each sample had two sites of pre-treatments: acid etched area and an area without treatment. After the biomaterials application, the samples were submitted to six cycles (demineralization: orange juice, 10 min; remineralization: artificial saliva, 1 h). Micro energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) mapping area analyses were performed after erosive cycling on both sites (n=84). μ-EDXRF mappings showed that artificial saliva and MWCNTO/GO/nHAp/F composite treatments produced lower dentin demineralization than in the other groups. Exposed dentin tubules allowed better interaction of nanobiomaterials than in smear layer covered dentin. Association of fluoride with other biomaterials had a positive influence on acid etched dentin. MWCNTO/GO/nHAp/F composite treatment resulted in levels of demineralization similar to the control group.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"9531 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129741646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Borys Mogilevych, D. Isensee, J. L. Rangel, C. Dal Pizzol, V. Martinello, G. Dieamant, A. Martin
Vitamins A, E and C play important role in skin homeostasis and protection. Hence, they are extensively used in many cosmetic and cosmeceutic products. However, their molecules are unstable, and do not easily penetrate into the skin, which drastically decreases its efficiency in topical formulations. Liposoluble derivative of the vitamin A - retinyl palmitate, vitamin E - tocopheryl acetate, and vitamin C - tetraisopalmitoyl ascorbic acid, are more stable, and are frequently used as an active ingredient in cosmetic products. Moreover, increased hydrophobicity of these molecules could lead to a higher skin penetration. The aim of this work is to track and compare the absorption of the liposoluble derivatives of the vitamins and their encapsulated form, into the healthy human skin in vivo. We used Confocal Raman Spectroscopy (CRS) that is proven to be helpful in label-free non-destructive investigation of the biochemical composition and molecular conformational analysis of the biological samples. The measurements were performed in the volar forearm of the 10 healthy volunteers. Skin was treated with both products, and Raman spectra were obtained after 15 min, 3 hours, and 6 hours after applying the formulation. 3510 Skin Composition Analyzer (River Diagnostics, The Netherlands) with 785 nm laser excitation was used to acquire information in the fingerprint region. Significant difference in permeation of the products was observed. Whereas only free form of retinyl palmitate penetrate the skin within first 15 minutes, all three vitamin derivatives were present under the skin surface in case of nanoparticulated form.
{"title":"Study of the vitamins A, E and C esters penetration into the skin by confocal Raman spectroscopy in vivo","authors":"Borys Mogilevych, D. Isensee, J. L. Rangel, C. Dal Pizzol, V. Martinello, G. Dieamant, A. Martin","doi":"10.1117/12.2181016","DOIUrl":"https://doi.org/10.1117/12.2181016","url":null,"abstract":"Vitamins A, E and C play important role in skin homeostasis and protection. Hence, they are extensively used in many cosmetic and cosmeceutic products. However, their molecules are unstable, and do not easily penetrate into the skin, which drastically decreases its efficiency in topical formulations. Liposoluble derivative of the vitamin A - retinyl palmitate, vitamin E - tocopheryl acetate, and vitamin C - tetraisopalmitoyl ascorbic acid, are more stable, and are frequently used as an active ingredient in cosmetic products. Moreover, increased hydrophobicity of these molecules could lead to a higher skin penetration. The aim of this work is to track and compare the absorption of the liposoluble derivatives of the vitamins and their encapsulated form, into the healthy human skin in vivo. We used Confocal Raman Spectroscopy (CRS) that is proven to be helpful in label-free non-destructive investigation of the biochemical composition and molecular conformational analysis of the biological samples. The measurements were performed in the volar forearm of the 10 healthy volunteers. Skin was treated with both products, and Raman spectra were obtained after 15 min, 3 hours, and 6 hours after applying the formulation. 3510 Skin Composition Analyzer (River Diagnostics, The Netherlands) with 785 nm laser excitation was used to acquire information in the fingerprint region. Significant difference in permeation of the products was observed. Whereas only free form of retinyl palmitate penetrate the skin within first 15 minutes, all three vitamin derivatives were present under the skin surface in case of nanoparticulated form.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131463364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. M. Silva, L. Alencar, M. Bernardi, F. A. S. Lima, C. Melo
In this work we investigate the scattering of light in means turbid in the presence or not of pigment and nanoparticles. For this we initially using a sample of collagen from means turbid with and without the presence of curcuma pigments and nanoparticles. Our results show that the light scattering is more intense in the samples with nanoparticles and curcuma pigment.
{"title":"Study of the scattering of the light in aqueous samples collagen in the presence of nanoparticles and curcuma pigment","authors":"F. M. Silva, L. Alencar, M. Bernardi, F. A. S. Lima, C. Melo","doi":"10.1117/12.2181121","DOIUrl":"https://doi.org/10.1117/12.2181121","url":null,"abstract":"In this work we investigate the scattering of light in means turbid in the presence or not of pigment and nanoparticles. For this we initially using a sample of collagen from means turbid with and without the presence of curcuma pigments and nanoparticles. Our results show that the light scattering is more intense in the samples with nanoparticles and curcuma pigment.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"187 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121193064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Quinto, C. B. Zamataro, C. Benetti, D. A. Dias, A. Blay, D. Zezell
Knowledge of dental structures is essential for understanding of laser interaction and its consequences during adhesion processes. Tubule density in dentin ranges from 4.900 to 90.000 per mm2, for diameters from 1 to 3 μm. Light propagation inside the tubules is associated with tubules orientation. To the best of our knowledge, there is no previous work in literature characterizing physical-chemical alterations in dentin. The dentin samples were irradiated with a Er,Cr:YSGG Laser at wavelength 2.78 μm, with an energy density of 9.46 J/cm2 , above the ablation threshold. ATRFTIR at wavenumbers 2000 to 700 cm-1 was used to evaluate the differences among third root region and tubules orientation.
{"title":"Optical properties of human radicular dentin: ATR-FTIR characterization and dentine tubule direction influence on radicular post adhesion","authors":"J. Quinto, C. B. Zamataro, C. Benetti, D. A. Dias, A. Blay, D. Zezell","doi":"10.1117/12.2181162","DOIUrl":"https://doi.org/10.1117/12.2181162","url":null,"abstract":"Knowledge of dental structures is essential for understanding of laser interaction and its consequences during adhesion processes. Tubule density in dentin ranges from 4.900 to 90.000 per mm2, for diameters from 1 to 3 μm. Light propagation inside the tubules is associated with tubules orientation. To the best of our knowledge, there is no previous work in literature characterizing physical-chemical alterations in dentin. The dentin samples were irradiated with a Er,Cr:YSGG Laser at wavelength 2.78 μm, with an energy density of 9.46 J/cm2 , above the ablation threshold. ATRFTIR at wavenumbers 2000 to 700 cm-1 was used to evaluate the differences among third root region and tubules orientation.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122257796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camila de Paula D´Almeida, C. Campos, Marcelo Saito Nogueira, S. Pratavieira, C. Kurachi
pathology. The optical properties of these intrinsic fluorophores respond to the microenvironment and the metabolic status, thus making fluorescence spectroscopy a valuable tool to study the conditions of biological tissues. The purpose of this study is to investigate the hairless mice skin metabolic changes during the photoaging process through lifetime and fluorescence measurements targeting NADH and FAD. Two lasers centered at 378 nm and 445 nm, respectively, perform excitation of NADH and FAD. The fluorescence acquisition is carried out at mice dorsal and ventral regions throughout the photoaging protocol and aging process. Differences in fluorescence and lifetime data between young and photoaged mice measurements were observed. The endogenous fluorescence spectrum of photoaged dorsal skin showed an increase compared to young and aged skin. Lifetime of bound NADH and free FAD presented an increase in the first week that continued until the end of the protocol. Aging process is being investigated to complement the information obtained from fluorescence data and lifetime of photoaging process.
{"title":"Time-resolved and steady-state fluorescence spectroscopy for the assessment of skin photoaging process","authors":"Camila de Paula D´Almeida, C. Campos, Marcelo Saito Nogueira, S. Pratavieira, C. Kurachi","doi":"10.1117/12.2180975","DOIUrl":"https://doi.org/10.1117/12.2180975","url":null,"abstract":"pathology. The optical properties of these intrinsic fluorophores respond to the microenvironment and the metabolic status, thus making fluorescence spectroscopy a valuable tool to study the conditions of biological tissues. The purpose of this study is to investigate the hairless mice skin metabolic changes during the photoaging process through lifetime and fluorescence measurements targeting NADH and FAD. Two lasers centered at 378 nm and 445 nm, respectively, perform excitation of NADH and FAD. The fluorescence acquisition is carried out at mice dorsal and ventral regions throughout the photoaging protocol and aging process. Differences in fluorescence and lifetime data between young and photoaged mice measurements were observed. The endogenous fluorescence spectrum of photoaged dorsal skin showed an increase compared to young and aged skin. Lifetime of bound NADH and free FAD presented an increase in the first week that continued until the end of the protocol. Aging process is being investigated to complement the information obtained from fluorescence data and lifetime of photoaging process.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124028567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. A. Gómez-García, A. Arranz, M. Fresno, M. Desco, U. Mahmood, J. Vaquero, J. Ripoll
Endoscopy is frequently used in the diagnosis of several gastro-intestinal pathologies as Crohn disease, ulcerative colitis or colorectal cancer. It has great potential as a non-invasive screening technique capable of detecting suspicious alterations in the intestinal mucosa, such as inflammatory processes. However, these early lesions usually cannot be detected with conventional endoscopes, due to lack of cellular detail and the absence of specific markers. Due to this lack of specificity, the development of new endoscopy technologies, which are able to show microscopic changes in the mucosa structure, are necessary. We here present a confocal endomicroscope, which in combination with a wide field fluorescence endoscope offers fast and specific macroscopic information through the use of activatable probes and a detailed analysis at cellular level of the possible altered tissue areas. This multi-modal and multi-scale imaging module, compatible with commercial endoscopes, combines near-infrared fluorescence (NIRF) measurements (enabling specific imaging of markers of disease and prognosis) and confocal endomicroscopy making use of a fiber bundle, providing a cellular level resolution. The system will be used in animal models exhibiting gastro-intestinal diseases in order to analyze the use of potential diagnostic markers in colorectal cancer. In this work, we present in detail the set-up design and the software implementation in order to obtain simultaneous RGB/NIRF measurements and short confocal scanning times.
{"title":"Fluorescence multi-scale endoscopy and its applications in the study and diagnosis of gastro-intestinal diseases: set-up design and software implementation","authors":"P. A. Gómez-García, A. Arranz, M. Fresno, M. Desco, U. Mahmood, J. Vaquero, J. Ripoll","doi":"10.1117/12.2189960","DOIUrl":"https://doi.org/10.1117/12.2189960","url":null,"abstract":"Endoscopy is frequently used in the diagnosis of several gastro-intestinal pathologies as Crohn disease, ulcerative colitis or colorectal cancer. It has great potential as a non-invasive screening technique capable of detecting suspicious alterations in the intestinal mucosa, such as inflammatory processes. However, these early lesions usually cannot be detected with conventional endoscopes, due to lack of cellular detail and the absence of specific markers. Due to this lack of specificity, the development of new endoscopy technologies, which are able to show microscopic changes in the mucosa structure, are necessary. We here present a confocal endomicroscope, which in combination with a wide field fluorescence endoscope offers fast and specific macroscopic information through the use of activatable probes and a detailed analysis at cellular level of the possible altered tissue areas. This multi-modal and multi-scale imaging module, compatible with commercial endoscopes, combines near-infrared fluorescence (NIRF) measurements (enabling specific imaging of markers of disease and prognosis) and confocal endomicroscopy making use of a fiber bundle, providing a cellular level resolution. The system will be used in animal models exhibiting gastro-intestinal diseases in order to analyze the use of potential diagnostic markers in colorectal cancer. In this work, we present in detail the set-up design and the software implementation in order to obtain simultaneous RGB/NIRF measurements and short confocal scanning times.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"117 44","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120826126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. C. Mota, Bruna A. Guerra, Brena S. A. Machado, A. J. Cabral, A. Gomes
Resin composites are widely used as restorative materials due to their excellent aesthetical and mechanical properties. Posterior teeth are constantly submitted to occlusal stress and upon restoration require more resistant resins. The aim of this study was to analyze in vitro the wear suffered over time by restorations in resin composite in posterior teeth, by Optical Coherence Tomography (OCT). 30 molars had occlusal cavities prepared and were randomly divided into three groups (n=10) and restored with resin composite: G1: Filtek P90 (3M/ESPE), G2: Tetric N-Ceram (Ivoclar Vivadent); G3: Filtek P60 (3M/ESPE). Specimens were subjected to initial analysis by OCT (OCP930SR, Thorlabs, axial resolution 6.2 μm) and stereoscopic microscope. Specimens were submitted to thermocycling (500 cycles, 5-55 °C) and subjected to simulated wear through a machine chewing movements (Wear Machine WM001), projecting four years of use. After mechanical cycles, the specimens were submitted to a second evaluation by the OCT and stereoscopic microscopy. As a result, it was observed that 90% of the restorations of both groups had fractures and/or points of stress concentration, considered niches for early dissemination of new fracture lines. It was also found that G1 and G2 had more points of stress concentration, whereas G3 had a higher incidence of fracture lines already propagated. It was concluded that the G3 showed more brittle behavior at the masticatory wear when compared to G1 and G2.
{"title":"Optical coherence tomography applied to the evaluation of wear of composite resin for posterior teeth","authors":"C. C. Mota, Bruna A. Guerra, Brena S. A. Machado, A. J. Cabral, A. Gomes","doi":"10.1117/12.2180753","DOIUrl":"https://doi.org/10.1117/12.2180753","url":null,"abstract":"Resin composites are widely used as restorative materials due to their excellent aesthetical and mechanical properties. Posterior teeth are constantly submitted to occlusal stress and upon restoration require more resistant resins. The aim of this study was to analyze in vitro the wear suffered over time by restorations in resin composite in posterior teeth, by Optical Coherence Tomography (OCT). 30 molars had occlusal cavities prepared and were randomly divided into three groups (n=10) and restored with resin composite: G1: Filtek P90 (3M/ESPE), G2: Tetric N-Ceram (Ivoclar Vivadent); G3: Filtek P60 (3M/ESPE). Specimens were subjected to initial analysis by OCT (OCP930SR, Thorlabs, axial resolution 6.2 μm) and stereoscopic microscope. Specimens were submitted to thermocycling (500 cycles, 5-55 °C) and subjected to simulated wear through a machine chewing movements (Wear Machine WM001), projecting four years of use. After mechanical cycles, the specimens were submitted to a second evaluation by the OCT and stereoscopic microscopy. As a result, it was observed that 90% of the restorations of both groups had fractures and/or points of stress concentration, considered niches for early dissemination of new fracture lines. It was also found that G1 and G2 had more points of stress concentration, whereas G3 had a higher incidence of fracture lines already propagated. It was concluded that the G3 showed more brittle behavior at the masticatory wear when compared to G1 and G2.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130751059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The pharyngitis and laryngitis are respiratory tract infections highly common. Pharyngitis can be accompanied by fever, especially if caused by a systemic infection. Laryngitis is an inflammation of your voice box (larynx) from irritation or infection. The conventional treatment is the antibiotics administration, which may be responsible by an increase of identification of bacterial strains resistant to drug. This fact associated to high incidence of these infections become important to develop new technologies for diagnosis. This study aims to evaluate the use of widefield fluorescence imaging for the characterization of oropharynx infections, in order to diagnose the bacteria colonization. The imaging system for wide field fluorescence visualization is Evince® (MMOptics, São Carlos, SP, Brazil) coupled to an Apple iPhone® cell phone device. The system consists of Light Emitting Diodes (LEDs) operating in the violet blue region centered at green-red spectrum 450 nm and optical filters that allow viewing of fluorescence. A tongue depressor was adapted to Evince® for mouth opening. The same images were captured with white light and fluorescence with an optical system. The red fluorescence may be a bacterial marker for physiological monitoring of oropharynx infection processes. The bacterial biofilm on tissue were assigned to the presence of protoporphyrin IX. This work indicates that the autofluorescence of the tissue may be used as a non-invasive technique to aid in the oropharynx infection diagnostic.
咽炎和喉炎是非常常见的呼吸道感染。咽炎可伴有发烧，特别是由全身感染引起的咽炎。喉炎是由刺激或感染引起的喉部炎症。传统的治疗方法是使用抗生素，这可能是由于增加了对药物耐药菌株的鉴定。与这些感染的高发病率相关的这一事实对于开发新的诊断技术非常重要。本研究旨在评价宽视场荧光成像在口咽部感染表征中的应用，以诊断细菌定植。用于宽视场荧光可视化的成像系统是Evince®(MMOptics, s o Carlos, SP, Brazil)与Apple iPhone®手机设备耦合。该系统由发光二极管(led)组成，工作在以450纳米绿红色光谱为中心的紫蓝色区域，以及允许观察荧光的光学滤光片。Evince®采用了压舌板进行开口。同样的图像是用光学系统用白光和荧光捕获的。红色荧光可能是生理监测口咽部感染过程的细菌标志物。组织上的细菌生物膜被分配到原卟啉IX的存在。这项工作表明，组织的自身荧光可以作为一种非侵入性技术来帮助口咽感染的诊断。
{"title":"Fluorescence diagnosis of upper respiratory tract infections","authors":"K. Blanco, N. Inada, C. Kurachi, V. Bagnato","doi":"10.1117/12.2180945","DOIUrl":"https://doi.org/10.1117/12.2180945","url":null,"abstract":"The pharyngitis and laryngitis are respiratory tract infections highly common. Pharyngitis can be accompanied by fever, especially if caused by a systemic infection. Laryngitis is an inflammation of your voice box (larynx) from irritation or infection. The conventional treatment is the antibiotics administration, which may be responsible by an increase of identification of bacterial strains resistant to drug. This fact associated to high incidence of these infections become important to develop new technologies for diagnosis. This study aims to evaluate the use of widefield fluorescence imaging for the characterization of oropharynx infections, in order to diagnose the bacteria colonization. The imaging system for wide field fluorescence visualization is Evince® (MMOptics, São Carlos, SP, Brazil) coupled to an Apple iPhone® cell phone device. The system consists of Light Emitting Diodes (LEDs) operating in the violet blue region centered at green-red spectrum 450 nm and optical filters that allow viewing of fluorescence. A tongue depressor was adapted to Evince® for mouth opening. The same images were captured with white light and fluorescence with an optical system. The red fluorescence may be a bacterial marker for physiological monitoring of oropharynx infection processes. The bacterial biofilm on tissue were assigned to the presence of protoporphyrin IX. This work indicates that the autofluorescence of the tissue may be used as a non-invasive technique to aid in the oropharynx infection diagnostic.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131024166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Traditional techniques to evaluate the aggregation of red blood cells by optical methods require large sample volume and provide parameters that vary significantly from one method to another. A simplified variant of a chip system previously developed by Shin et al. (2009)1 based on light transmission for measuring erythrocyte aggregation is presented. Through a detailed analysis of intensity versus time curves, relevant information about erythrocyte aggregation and its variables is obtained. Parameters that provide more accuracy for the diagnosis of patients in order to have an immediate application in Clinical Medicine are proposed.
{"title":"Simplified variant of an optical chip to evaluate aggregation of red blood cells","authors":"M. Toderi, B. Riquelme, H. Castellini","doi":"10.1117/12.2180907","DOIUrl":"https://doi.org/10.1117/12.2180907","url":null,"abstract":"Traditional techniques to evaluate the aggregation of red blood cells by optical methods require large sample volume and provide parameters that vary significantly from one method to another. A simplified variant of a chip system previously developed by Shin et al. (2009)1 based on light transmission for measuring erythrocyte aggregation is presented. Through a detailed analysis of intensity versus time curves, relevant information about erythrocyte aggregation and its variables is obtained. Parameters that provide more accuracy for the diagnosis of patients in order to have an immediate application in Clinical Medicine are proposed.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"536 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134090464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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