F. Carbinatto, N. Inada, Welington Lombardi, Natália Fernandez Cossetin, Cinthia Varoto, C. Kurachi, V. Bagnato
The use of portable electronic devices, in particular mobile phones such as smartphones is increasing not only for all known applications, but also for diagnosis of diseases and monitoring treatments like topical Photodynamic Therapy. The aim of the study is to evaluate the production of the photosensitizer Protoporphyrin IX (PpIX) after topical application of a cream containing methyl aminolevulinate (MAL) in the cervix with diagnosis of Cervical Intraepithelial Neoplasia (CIN) through the fluorescence images captured after one and three hours and compare the images using two devices (a Sony Xperia® mobile and an Apple Ipod®. Was observed an increasing fluorescence intensity of the cervix three hours after cream application, in both portable electronic devices. However, because was used a specific program for the treatment of images using the Ipod® device, these images presented better resolution than observed by the Sony cell phone without a specific program. One hour after cream application presented a more selective fluorescence than the group of three hours. In conclusion, the use of portable devices to obtain images of PpIX fluorescence shown to be an effective tool and is necessary the improvement of programs for achievement of better results.
{"title":"Comparison between two portable devices for widefield PpIX fluorescence during cervical intraepithelial neoplasia treatment","authors":"F. Carbinatto, N. Inada, Welington Lombardi, Natália Fernandez Cossetin, Cinthia Varoto, C. Kurachi, V. Bagnato","doi":"10.1117/12.2180914","DOIUrl":"https://doi.org/10.1117/12.2180914","url":null,"abstract":"The use of portable electronic devices, in particular mobile phones such as smartphones is increasing not only for all known applications, but also for diagnosis of diseases and monitoring treatments like topical Photodynamic Therapy. The aim of the study is to evaluate the production of the photosensitizer Protoporphyrin IX (PpIX) after topical application of a cream containing methyl aminolevulinate (MAL) in the cervix with diagnosis of Cervical Intraepithelial Neoplasia (CIN) through the fluorescence images captured after one and three hours and compare the images using two devices (a Sony Xperia® mobile and an Apple Ipod®. Was observed an increasing fluorescence intensity of the cervix three hours after cream application, in both portable electronic devices. However, because was used a specific program for the treatment of images using the Ipod® device, these images presented better resolution than observed by the Sony cell phone without a specific program. One hour after cream application presented a more selective fluorescence than the group of three hours. In conclusion, the use of portable devices to obtain images of PpIX fluorescence shown to be an effective tool and is necessary the improvement of programs for achievement of better results.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131562072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. R. de Sant' Anna, E. B. Nascimento, A. Higa, E. A. P. Santos, A. M. Espírito Santo, A. Martin
The in situ study evaluated antihistamine (DA) and bronchodilator(DB) drugs actions on dental enamel using FT Raman spectroscopy. Analysis of pH drugs were permorfed, DA 1.48 and DB 2.90. Enamel (n=24) were analysed by FT - Raman and randomly distributed in control group (CG) and experimental groups (GEA and GEB), specimens fixed in palatine appliances. In CG, dripped 20% sucrose (8Xday/3 min)/7 days. In GEA, 20% sucrose (8Xday/3 min) + drug (4Xday/3 min). In GEB, 20% sucrose (8Xday/3 min) B + drug (4Xday/3 min). FT- Raman analysis was performed again. Data analyzed by Student t test and ANOVA Differences in peak intensity of carbonate (CO3) /phosphate (PO4) (p<0.0286) post challenge in GEB related to baseline. The GEB integrated area of inorganic post challenge (p<0.0349).Organic loss in GEA, area (p<0.0228) and intensity (p<0. 0471) between moments and in GEB, area (p<0.0243) and intensity (p<0.00276). Comparing organic area and intensity difference observed post challenge GC (0,31±0,13) and GEB(0,54±0,13). Area difference between GEA(0,30±0,16) X GEB(0,54±0,13) and intensity difference GC(0,09172±0,04) and GEB(0,16±0,04) and GEA(0,091±0,05) X GEB(0,16±0,04). Significant integrated area CO3/PO4 loss for GC(0,12±0,01)X GEB(0,16±0,02) and GEB X GEA(0,13±0,02). For intensity differences were observed between GC(0,08±0,01) and GEB(0,10±0,01), and the last one and GEA(0,08±0,01) After administration of drugs, reduction in the amount of carbonate, organic and inorganic components were observed, denoting possible demineralization.
原位研究利用FT拉曼光谱评价了抗组胺药(DA)和支气管扩张药(DB)对牙釉质的作用。pH分析药物均经渗过，DA为1.48,DB为2.90。牙釉质(n=24)采用FT - Raman分析，随机分为对照组(CG)和实验组(GEA组和GEB组)，标本固定在腭矫治器上。在CG中，滴注20%蔗糖(8Xday/3 min)/7天。在GEA中，20%蔗糖(8Xday/3 min) +药物(4Xday/3 min)。在GEB中，20%蔗糖(8Xday/ 3min) B +药物(4Xday/ 3min)。再次进行FT- Raman分析。数据分析采用学生t检验和方差分析(ANOVA)分析，GEB攻击后碳酸盐(CO3) /磷酸盐(PO4)峰值强度与基线相关(p<0.0286)。GEB综合无机后挑战面积(p<0.0349)。土壤有机质损失、面积(p<0.0228)和强度(p< 0.05)。(0471)在矩和GEB中，面积(p<0.0243)和强度(p<0.00276)。比较攻毒后GC(0.31±0.13)和GEB(0.54±0.13)的有机面积和强度差异。GEA(0,30±0,16)X GEB(0,54±0,13)的面积差和GC(0,09172±0,04)与GEB(0,16±0,04)和GEA(0,091±0,05)X GEB(0,16±0,04)的强度差。GC(0,12±0.01)X GEB(0,16±0.02)和GEB X GEA(0,13±0.02)的综合面积CO3/PO4损失显著。GC(0.08±0.01)与GEB(0.010±0.01)、GC(0.08±0.01)与GEA(0.08±0.01)给药后碳酸盐、有机、无机成分均减少，提示可能脱矿。
{"title":"FT Raman spectroscopy in the study of human teeth under medications demineralization","authors":"G. R. de Sant' Anna, E. B. Nascimento, A. Higa, E. A. P. Santos, A. M. Espírito Santo, A. Martin","doi":"10.1117/12.2180782","DOIUrl":"https://doi.org/10.1117/12.2180782","url":null,"abstract":"The in situ study evaluated antihistamine (DA) and bronchodilator(DB) drugs actions on dental enamel using FT Raman spectroscopy. Analysis of pH drugs were permorfed, DA 1.48 and DB 2.90. Enamel (n=24) were analysed by FT - Raman and randomly distributed in control group (CG) and experimental groups (GEA and GEB), specimens fixed in palatine appliances. In CG, dripped 20% sucrose (8Xday/3 min)/7 days. In GEA, 20% sucrose (8Xday/3 min) + drug (4Xday/3 min). In GEB, 20% sucrose (8Xday/3 min) B + drug (4Xday/3 min). FT- Raman analysis was performed again. Data analyzed by Student t test and ANOVA Differences in peak intensity of carbonate (CO3) /phosphate (PO4) (p<0.0286) post challenge in GEB related to baseline. The GEB integrated area of inorganic post challenge (p<0.0349).Organic loss in GEA, area (p<0.0228) and intensity (p<0. 0471) between moments and in GEB, area (p<0.0243) and intensity (p<0.00276). Comparing organic area and intensity difference observed post challenge GC (0,31±0,13) and GEB(0,54±0,13). Area difference between GEA(0,30±0,16) X GEB(0,54±0,13) and intensity difference GC(0,09172±0,04) and GEB(0,16±0,04) and GEA(0,091±0,05) X GEB(0,16±0,04). Significant integrated area CO3/PO4 loss for GC(0,12±0,01)X GEB(0,16±0,02) and GEB X GEA(0,13±0,02). For intensity differences were observed between GC(0,08±0,01) and GEB(0,10±0,01), and the last one and GEA(0,08±0,01) After administration of drugs, reduction in the amount of carbonate, organic and inorganic components were observed, denoting possible demineralization.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132006931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The first Brazilian standard for UV protection sunglasses, NBR15111, was drafted and published in 2003, hitherto a faithful copy (mirror) of European, BSEN1836 standard. From 2010 to 2013 the Laboratório de Instrumentação Oftálmica of the School of Engineering of São Carlos (USP) made contribution in the review and drafting of this standard and the main change so far is on the extension of the UV range analysis for protection of sunglasses, i.e. from 280 - 380nm to 280-400nm. In previous studies, there are indications that ultraviolet protection degrades with use and exposure of sunglasses to natural ultraviolet radiation. Thus, this project aims to build a prototype for irradiating sunglasses lenses, where one of the spectacles will be submitted to the solar simulator; and the other to the prototype. This prototype consists of a panel with cover, which houses 100 lenses arranged in the vertical (user’s) position and which will be irradiated by the sun from sunrise until sunset. The lid opens automatically and should turn towards the sun, so that the lens will always be irradiated facing the sun. Sensors will be installed to close the cover and protect the lenses of undesirable weather conditions and to determine the ultraviolet index to which the lenses are being subjected to. The exposure time and UV index will be recorded and automatic opening or closing the lid may also be interfered by a PC by online software. Previously to irradiation, spectroscopy will be performed and then repeated after every 30 days of exposure.
巴西第一个防紫外线太阳镜标准NBR15111于2003年起草并发布，迄今为止是欧洲BSEN1836标准的忠实副本(镜像)。从2010年到2013年，卡洛斯工程学院(USP)的Laboratório de instrumenta o Oftálmica参与了该标准的审查和起草，到目前为止，主要的变化是扩展了太阳镜保护的紫外线范围分析，即从280- 380nm到280-400nm。在先前的研究中，有迹象表明，太阳镜在自然紫外线辐射下使用和暴露会降低紫外线防护能力。因此，该项目旨在建立一个辐照太阳镜镜片的原型，其中一个眼镜将提交给太阳模拟器;另一个是原型机。这个原型由一个带盖子的面板组成，上面有100个镜头排列在垂直(用户)位置，从日出到日落，这些镜头将被太阳照射。盖子自动打开，并应转向太阳，使镜头将始终面对太阳照射。在恶劣的天气条件下，将安装传感器关闭罩盖，保护镜片，并确定镜片所受的紫外线指数。曝光时间和紫外线指数将被记录，并可通过在线软件干扰PC机自动打开或关闭盖子。在辐照前，将进行光谱学检查，然后每30天后重复一次。
{"title":"Development of automated prototype for studying the effect of solar aging on sunglasses","authors":"L. M. Gomes, L. Ventura","doi":"10.1117/12.2181007","DOIUrl":"https://doi.org/10.1117/12.2181007","url":null,"abstract":"The first Brazilian standard for UV protection sunglasses, NBR15111, was drafted and published in 2003, hitherto a faithful copy (mirror) of European, BSEN1836 standard. From 2010 to 2013 the Laboratório de Instrumentação Oftálmica of the School of Engineering of São Carlos (USP) made contribution in the review and drafting of this standard and the main change so far is on the extension of the UV range analysis for protection of sunglasses, i.e. from 280 - 380nm to 280-400nm. In previous studies, there are indications that ultraviolet protection degrades with use and exposure of sunglasses to natural ultraviolet radiation. Thus, this project aims to build a prototype for irradiating sunglasses lenses, where one of the spectacles will be submitted to the solar simulator; and the other to the prototype. This prototype consists of a panel with cover, which houses 100 lenses arranged in the vertical (user’s) position and which will be irradiated by the sun from sunrise until sunset. The lid opens automatically and should turn towards the sun, so that the lens will always be irradiated facing the sun. Sensors will be installed to close the cover and protect the lenses of undesirable weather conditions and to determine the ultraviolet index to which the lenses are being subjected to. The exposure time and UV index will be recorded and automatic opening or closing the lid may also be interfered by a PC by online software. Previously to irradiation, spectroscopy will be performed and then repeated after every 30 days of exposure.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122910377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. C. Mota, L. Fernandes, L. D. Melo, D. S. Feitosa, Renata Cimões, A. Gomes
Imaging methods are widely used in diagnostic and among the diversity of modalities, optical coherence tomography (OCT) is nowadays commercially available and considered the most innovative technique used for imaging applications, in both medical and non-medical applications. In this study, we exploit the OCT technique in the oral cavity for identification and differentiation between free and attached gingiva, as well as determining the gingival phenotype, an important factor to determination of periodontal prognosis in patients. For the animal studies, five porcine jaws were analyzed using a Swept Source SS-OCT system operating at 1325nm and stereomicroscope, as gold pattern. The SSOCT at 1325nm was chosen due to the longer central wavelength, that allows to deeper penetration imaging, and the faster image acquisition, an essential factor for clinical setting. For the patient studies, a total of 30 males and female were examined using the SS-OCT at 1325nm and computer controlled periodontal probing. 2D and 3D images of tooth/gingiva interface were performed, and quantitative measurements of the gingival sulcus could be noninvasively obtained. Through the image analysis of the animals jaws, it was possible to quantify the free gingiva and the attached gingiva, the calculus deposition over teeth surface and also the subgingival calculus. For the patient’s studies, we demonstrated that the gingival phenotype could be measured without the periodontal probe introduction at the gingival sulcus, confirming that OCT can be potentially useful in clinic for direct observation and quantification of gingival phenotype in a non-invasive approach.
{"title":"Comparative analysis of gingival phenotype in animal and human experimental models using optical coherence tomography in a non-invasive approach","authors":"C. C. Mota, L. Fernandes, L. D. Melo, D. S. Feitosa, Renata Cimões, A. Gomes","doi":"10.1117/12.2180856","DOIUrl":"https://doi.org/10.1117/12.2180856","url":null,"abstract":"Imaging methods are widely used in diagnostic and among the diversity of modalities, optical coherence tomography (OCT) is nowadays commercially available and considered the most innovative technique used for imaging applications, in both medical and non-medical applications. In this study, we exploit the OCT technique in the oral cavity for identification and differentiation between free and attached gingiva, as well as determining the gingival phenotype, an important factor to determination of periodontal prognosis in patients. For the animal studies, five porcine jaws were analyzed using a Swept Source SS-OCT system operating at 1325nm and stereomicroscope, as gold pattern. The SSOCT at 1325nm was chosen due to the longer central wavelength, that allows to deeper penetration imaging, and the faster image acquisition, an essential factor for clinical setting. For the patient studies, a total of 30 males and female were examined using the SS-OCT at 1325nm and computer controlled periodontal probing. 2D and 3D images of tooth/gingiva interface were performed, and quantitative measurements of the gingival sulcus could be noninvasively obtained. Through the image analysis of the animals jaws, it was possible to quantify the free gingiva and the attached gingiva, the calculus deposition over teeth surface and also the subgingival calculus. For the patient’s studies, we demonstrated that the gingival phenotype could be measured without the periodontal probe introduction at the gingival sulcus, confirming that OCT can be potentially useful in clinic for direct observation and quantification of gingival phenotype in a non-invasive approach.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115511991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Reistad, Mallory Mayjonade, A. Ahadi, S. Andersson-Engels
Diffuse reflectance spectroscopy (DRS) is a rapid, non-invasive optical method widely adopted to gain diagnostic information of tissue. The most flexible approach to this method is a fiber-optic contact-probe used with a spectroscopy system. A challenge of this method is that the external pressure brought by the probe can significantly affect the tissue optical properties as well as the light coupling into the probe, and thus influence the collected DRS-spectrum. In this study we investigate and characterize the effect of probe pressure on DRS-spectra obtained with a calibrated loaded-spring system used with a fiber optic probe in the range (400 − 1600) nm. A multilayer FE-model of the indentation is developed to get a better insight of the distribution of pressure and stresses inside the skin under indentation.
{"title":"Characterization of probe contact effects on diffuse reflectance spectroscopy measurements","authors":"N. Reistad, Mallory Mayjonade, A. Ahadi, S. Andersson-Engels","doi":"10.1117/12.2180917","DOIUrl":"https://doi.org/10.1117/12.2180917","url":null,"abstract":"Diffuse reflectance spectroscopy (DRS) is a rapid, non-invasive optical method widely adopted to gain diagnostic information of tissue. The most flexible approach to this method is a fiber-optic contact-probe used with a spectroscopy system. A challenge of this method is that the external pressure brought by the probe can significantly affect the tissue optical properties as well as the light coupling into the probe, and thus influence the collected DRS-spectrum. In this study we investigate and characterize the effect of probe pressure on DRS-spectra obtained with a calibrated loaded-spring system used with a fiber optic probe in the range (400 − 1600) nm. A multilayer FE-model of the indentation is developed to get a better insight of the distribution of pressure and stresses inside the skin under indentation.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122574364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alessandra Rossi Paolillo, F. R. Paolillo, A. D. da Silva, R. Reiff, V. Bagnato, J. M. Alves
The bone fracture is important public health problems. The lasertherapy is used to accelerate tissue healing. Regarding diagnosis, few methods are validated to follow the evolution of bone microarchitecture. The aim of this study was to evaluate the effects of lasertherapy on bone repair with x-ray microtomography (μCT) and histomorphometry. A transverse rat tibia osteotomy with a Kirchner wire and a 2mm width polymeric spacer beads were used to produce a delayed bone union. Twelve rats were divided into two groups: (i) Control Group: untreated fracture and; (ii) Laser Group: fracture treated with laser. Twelve sessions of treatment (808nm laser, 100mW, 125J/cm2, 50seconds) were performed. The μCT scanner parameters were: 100kV, 100μA, Al+Cu filter and 9.92μm resolution. A volume of interest (VOI) was chosen with 300 sections above and below the central region of the fracture, totaling 601sections with a 5.96mm. The softwares CT-Analyzer, NRecon and Mimics were used for 2D and 3D analysis. A histomorphometry analysis was also performed. The connectivity (Conn) showed significant increase for Laser Group than Control Group (32371±20689 vs 17216±9467, p<0.05). There was no significant difference for bone volume (59±19mm3 vs 47± 8mm3) and histomorfometric data [Laser and Control Groups showed greater amount of cartilaginous (0.19±0.05% vs 0.11±0.09%) and fibrotic (0.21±0.12% vs 0.09±0.11%) tissues]. The negative effect was presence of the cartilaginous and fibrotic tissues which may be related to the Kirchner wire and the non-absorption of the polymeric that may have influenced negatively the light distribution through the bone. However, the positive effect was greater bone connectivity, indicating improvement in bone microarchitecture.
骨折是重要的公共卫生问题。激光疗法用于加速组织愈合。在诊断方面，很少有方法被证实可以跟踪骨微结构的演变。本研究采用x射线显微断层扫描(μCT)和组织形态学测量法评价激光治疗对骨修复的影响。采用Kirchner钢丝和2mm宽度的聚合物间隔珠进行大鼠胫骨横截骨，以延迟骨愈合。12只大鼠分为两组:(i)对照组:骨折未治疗组;(ii)激光组:用激光治疗骨折。治疗12次(808nm激光，100mW, 125J/cm2, 50秒)。μCT扫描参数为:100kV, 100μA, Al+Cu滤波器，9.92μm分辨率。在裂缝中心区域的上方和下方选择了300个断面，共601个断面，宽度为5.96mm。采用CT-Analyzer、NRecon和Mimics软件进行二维和三维分析。同时进行组织形态学分析。激光组的连通性(Conn)显著高于对照组(32371±20689 vs 17216±9467,p<0.05)。两组骨体积(59±19mm3 vs 47±8mm3)和组织形态学数据无显著差异[激光组和对照组软骨组织(0.19±0.05% vs 0.11±0.09%)和纤维化组织(0.21±0.12% vs 0.09±0.11%)较多]。负面影响是软骨和纤维化组织的存在，这可能与基什内尔丝有关，聚合物的不吸收可能对通过骨骼的光分布产生负面影响。然而，积极的影响是更大的骨连通性，表明骨微结构的改善。
{"title":"Effects of infrared laser on the bone repair assessed by x-ray microtomography (μct) and histomorphometry","authors":"Alessandra Rossi Paolillo, F. R. Paolillo, A. D. da Silva, R. Reiff, V. Bagnato, J. M. Alves","doi":"10.1117/12.2180804","DOIUrl":"https://doi.org/10.1117/12.2180804","url":null,"abstract":"The bone fracture is important public health problems. The lasertherapy is used to accelerate tissue healing. Regarding diagnosis, few methods are validated to follow the evolution of bone microarchitecture. The aim of this study was to evaluate the effects of lasertherapy on bone repair with x-ray microtomography (μCT) and histomorphometry. A transverse rat tibia osteotomy with a Kirchner wire and a 2mm width polymeric spacer beads were used to produce a delayed bone union. Twelve rats were divided into two groups: (i) Control Group: untreated fracture and; (ii) Laser Group: fracture treated with laser. Twelve sessions of treatment (808nm laser, 100mW, 125J/cm2, 50seconds) were performed. The μCT scanner parameters were: 100kV, 100μA, Al+Cu filter and 9.92μm resolution. A volume of interest (VOI) was chosen with 300 sections above and below the central region of the fracture, totaling 601sections with a 5.96mm. The softwares CT-Analyzer, NRecon and Mimics were used for 2D and 3D analysis. A histomorphometry analysis was also performed. The connectivity (Conn) showed significant increase for Laser Group than Control Group (32371±20689 vs 17216±9467, p<0.05). There was no significant difference for bone volume (59±19mm3 vs 47± 8mm3) and histomorfometric data [Laser and Control Groups showed greater amount of cartilaginous (0.19±0.05% vs 0.11±0.09%) and fibrotic (0.21±0.12% vs 0.09±0.11%) tissues]. The negative effect was presence of the cartilaginous and fibrotic tissues which may be related to the Kirchner wire and the non-absorption of the polymeric that may have influenced negatively the light distribution through the bone. However, the positive effect was greater bone connectivity, indicating improvement in bone microarchitecture.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126909216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. F. Menezes, M. B. Requena, R. Lizarelli, V. Bagnato
Blue LED system irradiation shows many important properties on skin as: bacterial decontamination, degradation of endogenous skin chromophores and biostimulation. In this clinical study we prove that the blue light improves the skin hydration. In the literature none authors reports this biological property on skin. Then this study aims to discuss the role of blue light in the skin hydration. Twenty patients were selected to this study with age between 25-35 years old and phototype I, II and III. A defined area from forearm was pre determined (A = 4.0 cm2). The study was randomized in two treatment groups using one blue light device (power of 5.3mW and irradiance of 10.8mW/cm2). The first treatment group was irradiated with 3J/cm2 (277seconds) and the second with 6J/cm2 (555 seconds). The skin hydration evaluations were done using a corneometer. The measurements were collected in 7, 14, 21 and 30 days, during the treatment. Statistical test of ANOVA, Tukey and T-Student were applied considering 5% of significance. In conclusion, both doses were able to improve the skin hydration; however, 6J/cm2 has kept this hydration for 30 days.
{"title":"Blue LED irradiation to hydration of skin","authors":"P. F. Menezes, M. B. Requena, R. Lizarelli, V. Bagnato","doi":"10.1117/12.2181196","DOIUrl":"https://doi.org/10.1117/12.2181196","url":null,"abstract":"Blue LED system irradiation shows many important properties on skin as: bacterial decontamination, degradation of endogenous skin chromophores and biostimulation. In this clinical study we prove that the blue light improves the skin hydration. In the literature none authors reports this biological property on skin. Then this study aims to discuss the role of blue light in the skin hydration. Twenty patients were selected to this study with age between 25-35 years old and phototype I, II and III. A defined area from forearm was pre determined (A = 4.0 cm2). The study was randomized in two treatment groups using one blue light device (power of 5.3mW and irradiance of 10.8mW/cm2). The first treatment group was irradiated with 3J/cm2 (277seconds) and the second with 6J/cm2 (555 seconds). The skin hydration evaluations were done using a corneometer. The measurements were collected in 7, 14, 21 and 30 days, during the treatment. Statistical test of ANOVA, Tukey and T-Student were applied considering 5% of significance. In conclusion, both doses were able to improve the skin hydration; however, 6J/cm2 has kept this hydration for 30 days.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126250261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. B. Govone, P. A. Gómez-García, A. Carvalho, R. Capuzzo, D. Magalhães, C. Kurachi
Metastasis is one of the major cancer complications, since the malignant cells detach from the primary tumor and reaches other organs or tissues. The sentinel lymph node (SLN) is the first lymphatic structure to be affected by the malignant cells, but its location is still a great challenge for the medical team. This occurs due to the fact that the lymph nodes are located between the muscle fibers, making it visualization difficult. Seeking to aid the surgeon in the detection of the SLN, the present study aims to develop a widefield fluorescence imaging device using the indocyanine green as fluorescence marker. The system is basically composed of a 780nm illumination unit, optical components for 810nm fluorescence detection, two CCD cameras, a laptop, and dedicated software. The illumination unit has 16 diode lasers. A dichroic mirror and bandpass filters select and deliver the excitation light to the interrogated tissue, and select and deliver the fluorescence light to the camera. One camera is responsible for the acquisition of visible light and the other one for the acquisition of the ICG fluorescence. The software developed at the LabVIEW® platform generates a real time merged image where it is possible to observe the fluorescence spots, related to the lymph nodes, superimposed at the image under white light. The system was tested in a mice model, and a first patient with tongue cancer was imaged. Both results showed the potential use of the presented fluorescence imaging system assembled for sentinel lymph node detection.
{"title":"Portable widefield imaging device for ICG-detection of the sentinel lymph node","authors":"A. B. Govone, P. A. Gómez-García, A. Carvalho, R. Capuzzo, D. Magalhães, C. Kurachi","doi":"10.1117/12.2181025","DOIUrl":"https://doi.org/10.1117/12.2181025","url":null,"abstract":"Metastasis is one of the major cancer complications, since the malignant cells detach from the primary tumor and reaches other organs or tissues. The sentinel lymph node (SLN) is the first lymphatic structure to be affected by the malignant cells, but its location is still a great challenge for the medical team. This occurs due to the fact that the lymph nodes are located between the muscle fibers, making it visualization difficult. Seeking to aid the surgeon in the detection of the SLN, the present study aims to develop a widefield fluorescence imaging device using the indocyanine green as fluorescence marker. The system is basically composed of a 780nm illumination unit, optical components for 810nm fluorescence detection, two CCD cameras, a laptop, and dedicated software. The illumination unit has 16 diode lasers. A dichroic mirror and bandpass filters select and deliver the excitation light to the interrogated tissue, and select and deliver the fluorescence light to the camera. One camera is responsible for the acquisition of visible light and the other one for the acquisition of the ICG fluorescence. The software developed at the LabVIEW® platform generates a real time merged image where it is possible to observe the fluorescence spots, related to the lymph nodes, superimposed at the image under white light. The system was tested in a mice model, and a first patient with tongue cancer was imaged. Both results showed the potential use of the presented fluorescence imaging system assembled for sentinel lymph node detection.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127223999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. D. Stringasci, L. Moriyama, A. G. Salvio, V. Bagnato, C. Kurachi
Cancer is responsible for about 13% of all causes of death in the world. Over 7 million people die annually of this disease. In most cases, the survival rates are greater when diagnosed in early stages. It is known that tumor lesions present a different temperature compared with the normal tissues. Some studies have been performed in an attempt to establish new diagnosis methods, targeting this temperature difference. In this study, we aim to investigate the use of a handheld thermographic camera to discriminate skin lesions. The patients presenting Basal Cell Carcinoma, Squamous Cell Carcinoma, Actinic Keratosis, Pigmented Seborrheic Keratosis, Melanoma or Intradermal Nevus lesions have been investigated at the Skin Departament of Amaral Carvalho Hospital. Patients are selected by a dermatologist, and the lesion images are recorded using an infrared camera. The images are evaluated taken into account the temperature level, and differences into lesion areas, borders, and between altered and normal skin. The present results show that thermography may be an important tool for aiding in the clinical diagnostics of superficial skin lesions.
{"title":"Thermographic diagnostics to discriminate skin lesions: a clinical study","authors":"M. D. Stringasci, L. Moriyama, A. G. Salvio, V. Bagnato, C. Kurachi","doi":"10.1117/12.2180967","DOIUrl":"https://doi.org/10.1117/12.2180967","url":null,"abstract":"Cancer is responsible for about 13% of all causes of death in the world. Over 7 million people die annually of this disease. In most cases, the survival rates are greater when diagnosed in early stages. It is known that tumor lesions present a different temperature compared with the normal tissues. Some studies have been performed in an attempt to establish new diagnosis methods, targeting this temperature difference. In this study, we aim to investigate the use of a handheld thermographic camera to discriminate skin lesions. The patients presenting Basal Cell Carcinoma, Squamous Cell Carcinoma, Actinic Keratosis, Pigmented Seborrheic Keratosis, Melanoma or Intradermal Nevus lesions have been investigated at the Skin Departament of Amaral Carvalho Hospital. Patients are selected by a dermatologist, and the lesion images are recorded using an infrared camera. The images are evaluated taken into account the temperature level, and differences into lesion areas, borders, and between altered and normal skin. The present results show that thermography may be an important tool for aiding in the clinical diagnostics of superficial skin lesions.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"9531 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130586028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kagel, Julia Honselmann Genannt Humme, E. Rosa, R. Turchiello, Arandi Ginane Bezerra Junior
In the present study we report on the ability of gold nanoparticles (AuNP) to enhance the antimicrobial activity of the photosensitizer Rose Bengal (RB), a very effective singlet oxygen generator. Our experiments were conducted using a suspension of Escherichia Coli in the presence of either RB or a combination of RB and AuNP. Nanoparticles were synthesized by laser ablation in water, which allows high purity, biologically friendly AuNP production, as compared to traditional chemical methods. Several relative concentrations of bacteria, photosensitizes and AuNP were studied. Bacterial survival rates were determined before and after LED light illumination. The phototoxicity of RB with and without AuNP was checked following illumination for 10 and 20 minutes. As a control, the dark toxicity of RB was verified. The results show that the survival rate of bacteria decreases significantly with the increase of RB concentration and illumination time, which is in accordance with previous works. Interestingly, our results also indicate a significant increase in the lethal photosensitization of RB in the presence of AuNP. We propose this effect is due to plasmonic light enhancement, considering the superposition of RB and AuNP absorption spectra, which favors electric field enhancement effects in the presence of AuNP. Similar experiments using the photosensitizer Methylene Blue (MB) allowed us to test our hypothesis for MB did not show any difference in its phototoxicity in the presence of AuNP. We propose this observed synergistic effect could be an effective way for improving photodynamic inactivation of microorganisms.
{"title":"Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles","authors":"H. Kagel, Julia Honselmann Genannt Humme, E. Rosa, R. Turchiello, Arandi Ginane Bezerra Junior","doi":"10.1117/12.2180947","DOIUrl":"https://doi.org/10.1117/12.2180947","url":null,"abstract":"In the present study we report on the ability of gold nanoparticles (AuNP) to enhance the antimicrobial activity of the photosensitizer Rose Bengal (RB), a very effective singlet oxygen generator. Our experiments were conducted using a suspension of Escherichia Coli in the presence of either RB or a combination of RB and AuNP. Nanoparticles were synthesized by laser ablation in water, which allows high purity, biologically friendly AuNP production, as compared to traditional chemical methods. Several relative concentrations of bacteria, photosensitizes and AuNP were studied. Bacterial survival rates were determined before and after LED light illumination. The phototoxicity of RB with and without AuNP was checked following illumination for 10 and 20 minutes. As a control, the dark toxicity of RB was verified. The results show that the survival rate of bacteria decreases significantly with the increase of RB concentration and illumination time, which is in accordance with previous works. Interestingly, our results also indicate a significant increase in the lethal photosensitization of RB in the presence of AuNP. We propose this effect is due to plasmonic light enhancement, considering the superposition of RB and AuNP absorption spectra, which favors electric field enhancement effects in the presence of AuNP. Similar experiments using the photosensitizer Methylene Blue (MB) allowed us to test our hypothesis for MB did not show any difference in its phototoxicity in the presence of AuNP. We propose this observed synergistic effect could be an effective way for improving photodynamic inactivation of microorganisms.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132798202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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