R. Lizarelli, Natália D. P. Grandi, F. Florez, C. Grecco, L. A. Lopes
Skin hydration is important to prevent aging and dysfunction of orofacial system. Nowadays, it is known that cutaneous system is linked to muscle system, then every dentist need to treat healthy facial skin, as lips, keeping orofacial functions healthy. Thirty-two patients were treated using laser and led therapy single or associated to biomaterials (dermo-cosmetics) searching for the best protocol to promote skin hydration. Using a peace of equipment to measure electric impedance, percentage of water and oil from skin, before and after different treatments were analyzed. Statistic tests using 5% and 0.1% of significance were applied and results showed that light could improve hydration of epidermis layer of facial skin. Considering just light effect, using infrared laser followed by blue led system is more effective to hydration than just blue led system application. Considering dermo-cosmetic and light, the association between both presented the best result.
{"title":"Clinical study on orofacial photonic hydration using phototherapy and biomaterials","authors":"R. Lizarelli, Natália D. P. Grandi, F. Florez, C. Grecco, L. A. Lopes","doi":"10.1117/12.2181132","DOIUrl":"https://doi.org/10.1117/12.2181132","url":null,"abstract":"Skin hydration is important to prevent aging and dysfunction of orofacial system. Nowadays, it is known that cutaneous system is linked to muscle system, then every dentist need to treat healthy facial skin, as lips, keeping orofacial functions healthy. Thirty-two patients were treated using laser and led therapy single or associated to biomaterials (dermo-cosmetics) searching for the best protocol to promote skin hydration. Using a peace of equipment to measure electric impedance, percentage of water and oil from skin, before and after different treatments were analyzed. Statistic tests using 5% and 0.1% of significance were applied and results showed that light could improve hydration of epidermis layer of facial skin. Considering just light effect, using infrared laser followed by blue led system is more effective to hydration than just blue led system application. Considering dermo-cosmetic and light, the association between both presented the best result.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121631984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Optical Coherence Tomography (OCT) is a noninvasive imaging technique with high resolution widely used for in vivo applications. Nonetheless, OCT is prone to speckle, a granular noise that degrades the OCT signal. Speckle statistics may, nevertheless, reveal information regarding the scatterers from which it originates. This fact is exploited by techniques such as Speckle Variance-OCT (SVOCT). SVOCT, however, doesn’t provide quantitative information, which is a major drawback for the use of speckle based techniques on OCT. In the present work we attack this problem, proposing a new method for analysis of speckle in OCT signal, based on autocorrelation. We associate the changes in decorrelation time of the signal with the changes in flow velocity. It is expected that greater velocities result in lower decorrelation times. To verify that, milk was pumped through a microchannel at different velocities, and the decorrelation time was computed for a single point in the center of the microchannel, sampled at 8 kHz rate. Our results suggest that for flows rates greater than 1 μl/min it is possible to associate decorrelation time with flow velocity, while velocities below that value are not distinguishable, supposedly due to the Brownian motion. For flow rates above 50 μl/min our acquisition rate doesn’t get enough sampling information, as the decorrelation time gets too low. These results indicate that Speckle based techniques may be used to get quantitative information of flow in OCT samples, which can be used to assist in many diagnostics modalities, as well as map such flow regions.
光学相干层析成像(OCT)是一种无创的高分辨率成像技术，广泛应用于体内。尽管如此，OCT容易产生斑点，这是一种降低OCT信号的颗粒噪声。然而，散斑统计可以揭示有关其起源的散射体的信息。这一事实被诸如散斑方差- oct (SVOCT)之类的技术所利用。然而，SVOCT不能提供定量信息，这是基于散斑技术在OCT上使用的一个主要缺点。在本工作中，我们解决了这个问题，提出了一种基于自相关的OCT信号中散斑分析的新方法。我们将信号去相关时间的变化与流速的变化联系起来。预计更大的速度会导致更短的去相关时间。为了验证这一点，以不同的速度泵送牛奶通过微通道，并以8 kHz的采样率计算微通道中心单个点的去相关时间。我们的结果表明，当流速大于1 μl/min时，可以将解相关时间与流速相关联，而低于该值的流速则无法区分，可能是由于布朗运动的原因。当流量大于50 μl/min时，由于去相关时间过低，我们的采集速率无法获得足够的采样信息。这些结果表明，基于斑点的技术可用于获得OCT样品中流量的定量信息，这些信息可用于辅助许多诊断模式，以及绘制此类流动区域。
{"title":"New speckle analysis method for optical coherence tomography signal based on autocorrelation","authors":"L. D. De Pretto, G. Nogueira, A. Freitas","doi":"10.1117/12.2180813","DOIUrl":"https://doi.org/10.1117/12.2180813","url":null,"abstract":"Optical Coherence Tomography (OCT) is a noninvasive imaging technique with high resolution widely used for in vivo applications. Nonetheless, OCT is prone to speckle, a granular noise that degrades the OCT signal. Speckle statistics may, nevertheless, reveal information regarding the scatterers from which it originates. This fact is exploited by techniques such as Speckle Variance-OCT (SVOCT). SVOCT, however, doesn’t provide quantitative information, which is a major drawback for the use of speckle based techniques on OCT. In the present work we attack this problem, proposing a new method for analysis of speckle in OCT signal, based on autocorrelation. We associate the changes in decorrelation time of the signal with the changes in flow velocity. It is expected that greater velocities result in lower decorrelation times. To verify that, milk was pumped through a microchannel at different velocities, and the decorrelation time was computed for a single point in the center of the microchannel, sampled at 8 kHz rate. Our results suggest that for flows rates greater than 1 μl/min it is possible to associate decorrelation time with flow velocity, while velocities below that value are not distinguishable, supposedly due to the Brownian motion. For flow rates above 50 μl/min our acquisition rate doesn’t get enough sampling information, as the decorrelation time gets too low. These results indicate that Speckle based techniques may be used to get quantitative information of flow in OCT samples, which can be used to assist in many diagnostics modalities, as well as map such flow regions.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133981532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liposomes have been used to deliver DNA, drugs and, more recently, nanoparticles such as quantum dots, into living cells. Their electrostatic interaction with cell’s surface (negatively charged) can lead to membrane destabilization and/or fusion, facilitating intracellular release of those compounds. Nevertheless, cationic lipids can modify living cells homeostasis, depending on their concentration. In this study, we observed that the DOTAP cationic lipid concentrations influence the red blood cells (RBCs) homeostasis. We used fluorescent fusogenic liposomes composed by three lipids: DOPE, DOTAP and DPPE-Rhodamine (1:0.1/0.3/0.5/0.8/1:0.1 mM respectively), varying DOTAP from 0.1 to 1 mM. To probe liposomes ability to fuse with cells, RBCs (1% in saline) were utilized. Liposomes were characterized by zeta potential, dynamic light scattering (DLS), fluorescence and transmission electron microscopy. Their interaction with RBCs was evaluated by fluorescence microscopy and flow cytometry. Zeta potential results showed that, from 0.1 to 1 mM concentration, the charge increases, due to the addition of DOTAP. Liposomes’ diameter does not vary significantly when more DOTAP was added, except for the one containing 0.1 mM of DOTAP, according to DLS results. Flow cytometry and microscopy analysis showed that for all DOTAP’ concentration applied, the liposomes were capable to label RBCs. However, as higher the amount of DOTAP in liposomes, the more harmful they were to cells. Thus, the results showed that it is possible to use lower concentrations of DOTAP keeping the fusogenic liposomes’s ability and cell homeostasis. This is important to guarantee a greater efficiency in the delivery of nanoparticles or other active samples into cells.
脂质体已被用于将DNA、药物以及最近的量子点等纳米粒子输送到活细胞中。它们与细胞表面(带负电荷)的静电相互作用可导致膜不稳定和/或融合，促进细胞内释放这些化合物。然而，阳离子脂质可以改变活细胞的稳态，这取决于它们的浓度。在这项研究中，我们观察到DOTAP阳离子脂质浓度影响红细胞(rbc)稳态。我们使用由三种脂质组成的荧光融合脂质体:DOPE, DOTAP和dpe -罗丹明(分别为1:0.1/0.3/0.5/0.8/1:0.1 mM)， DOTAP从0.1到1 mM不等。为了探测脂质体与细胞融合的能力，使用了红细胞(1%盐水)。采用zeta电位、动态光散射(DLS)、荧光和透射电镜对脂质体进行表征。通过荧光显微镜和流式细胞术评估它们与红细胞的相互作用。Zeta电位结果表明，在0.1 ~ 1mm浓度范围内，DOTAP的加入使电荷增加。DLS结果显示，除了含有0.1 mM DOTAP的脂质体外，添加更多DOTAP的脂质体直径没有显著变化。流式细胞术和显微镜分析显示，对于所有浓度的DOTAP，脂质体都能够标记红细胞。然而，脂质体中DOTAP含量越高，对细胞的危害越大。因此，结果表明，可以使用较低浓度的DOTAP来保持促聚变脂质体的能力和细胞稳态。这对于保证纳米颗粒或其他活性样品进入细胞的更高效率是很重要的。
{"title":"Fluorescent liposomes to probe how DOTAP lipid concentrations can change red blood cells homeostasis","authors":"A. L. Matos, G. Pereira, B. S. Santos, A. Fontes","doi":"10.1117/12.2180957","DOIUrl":"https://doi.org/10.1117/12.2180957","url":null,"abstract":"Liposomes have been used to deliver DNA, drugs and, more recently, nanoparticles such as quantum dots, into living cells. Their electrostatic interaction with cell’s surface (negatively charged) can lead to membrane destabilization and/or fusion, facilitating intracellular release of those compounds. Nevertheless, cationic lipids can modify living cells homeostasis, depending on their concentration. In this study, we observed that the DOTAP cationic lipid concentrations influence the red blood cells (RBCs) homeostasis. We used fluorescent fusogenic liposomes composed by three lipids: DOPE, DOTAP and DPPE-Rhodamine (1:0.1/0.3/0.5/0.8/1:0.1 mM respectively), varying DOTAP from 0.1 to 1 mM. To probe liposomes ability to fuse with cells, RBCs (1% in saline) were utilized. Liposomes were characterized by zeta potential, dynamic light scattering (DLS), fluorescence and transmission electron microscopy. Their interaction with RBCs was evaluated by fluorescence microscopy and flow cytometry. Zeta potential results showed that, from 0.1 to 1 mM concentration, the charge increases, due to the addition of DOTAP. Liposomes’ diameter does not vary significantly when more DOTAP was added, except for the one containing 0.1 mM of DOTAP, according to DLS results. Flow cytometry and microscopy analysis showed that for all DOTAP’ concentration applied, the liposomes were capable to label RBCs. However, as higher the amount of DOTAP in liposomes, the more harmful they were to cells. Thus, the results showed that it is possible to use lower concentrations of DOTAP keeping the fusogenic liposomes’s ability and cell homeostasis. This is important to guarantee a greater efficiency in the delivery of nanoparticles or other active samples into cells.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"399 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124743534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Crispim, Adriano Bogar, N. Allemann, J. C. C. Neto, W. Chamon
Introduction: To date, it has never been demonstrated the propagation sound speed in human corneas and lens in vivo. With the advent of Optical Coherence Tomography (OCT), it became possible to determine the dimensions of the ocular tissues without the interference of sound propagation speed and to use this information to define the real propagation sound speed for each patient and individualized structure. Aim: To determine the sound propagation speed in the cornea and lens from patients that theoretically exhibits differences in tissue elasticity (normal corneas and keratoconus, corneas of young and elderly patients, in addition to clear crystalline lens from young and elderly patients with cataract). Then, relate the determined velocity in each group with the expected tissue elasticity of the cornea and lens. Methods: We studied 100 eyes from 50 patients: 50 with keratoconus and no cataract and 50 with cataract and no corneal changes. All patients measured corneal and lens thickness by ultrasound methods (Ultrasonic Biomicroscopy - UBM and Ultrasonic Pachymetry - USP) and by OCT (RTVue®, Lenstar® and Visante®), then were divided into 2 groups: Group 1 (Cornea) analyzed the central corneal thickness (UBM, USP, RTVue®, Visante®, Lenstar®); Group 2 (Lens) analyzed the axial thickness of the lens (UBM and Lenstar®). Based on standard ultrasonic speed from USP (1640 m/s) and UBM (1548 m/s), we calculated the real sound propagation speed in each tissue. Results: Based on USP, the corneal sound speed on control group (1616 m/s) was faster than on keratoconus group (1547 m/s) (P < 0.0001). Based on UBM, the lens sound speed on cataract group (1664 m/s) was faster that on control group (1605 m/s) (P < 0.0001). Discussion: It is known that sound propagates faster in materials with lower elasticity. We found that the sound speed on keratoconic corneas (high elasticity) was slower and on cataract lens (lower elasticity) was faster than normal corneas and lens in vivo.
{"title":"Evaluation of eye tissue elasticity by means of sound propagation speed measuring in vivo","authors":"J. Crispim, Adriano Bogar, N. Allemann, J. C. C. Neto, W. Chamon","doi":"10.1117/12.2179598","DOIUrl":"https://doi.org/10.1117/12.2179598","url":null,"abstract":"Introduction: To date, it has never been demonstrated the propagation sound speed in human corneas and lens in vivo. With the advent of Optical Coherence Tomography (OCT), it became possible to determine the dimensions of the ocular tissues without the interference of sound propagation speed and to use this information to define the real propagation sound speed for each patient and individualized structure. Aim: To determine the sound propagation speed in the cornea and lens from patients that theoretically exhibits differences in tissue elasticity (normal corneas and keratoconus, corneas of young and elderly patients, in addition to clear crystalline lens from young and elderly patients with cataract). Then, relate the determined velocity in each group with the expected tissue elasticity of the cornea and lens. Methods: We studied 100 eyes from 50 patients: 50 with keratoconus and no cataract and 50 with cataract and no corneal changes. All patients measured corneal and lens thickness by ultrasound methods (Ultrasonic Biomicroscopy - UBM and Ultrasonic Pachymetry - USP) and by OCT (RTVue®, Lenstar® and Visante®), then were divided into 2 groups: Group 1 (Cornea) analyzed the central corneal thickness (UBM, USP, RTVue®, Visante®, Lenstar®); Group 2 (Lens) analyzed the axial thickness of the lens (UBM and Lenstar®). Based on standard ultrasonic speed from USP (1640 m/s) and UBM (1548 m/s), we calculated the real sound propagation speed in each tissue. Results: Based on USP, the corneal sound speed on control group (1616 m/s) was faster than on keratoconus group (1547 m/s) (P < 0.0001). Based on UBM, the lens sound speed on cataract group (1664 m/s) was faster that on control group (1605 m/s) (P < 0.0001). Discussion: It is known that sound propagates faster in materials with lower elasticity. We found that the sound speed on keratoconic corneas (high elasticity) was slower and on cataract lens (lower elasticity) was faster than normal corneas and lens in vivo.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126304114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lauren E. Jamieson, A. P. Bell, David J. Harrison, Colin Campbell
Cellular redox potential is important for the control and regulation of a vast number of processes occurring in cells. When the fine redox potential balance within cells is disturbed it can have serious consequences such as the initiation or progression of disease. It is thought that a redox gradient develops in cancer tumours where the peripheral regions are well oxygenated and internal regions, further from vascular blood supply, become starved of oxygen and hypoxic. This makes treatment of these areas more challenging as, for example, radiotherapy relies on the presence of oxygen. Currently techniques for quantitative analysis of redox gradients are limited. Surface enhanced Raman scattering (SERS) nanosensors (NS) have been used to detect redox potential in a quantitative manner in monolayer cultured cells with many advantages over other techniques. This technique has considerable potential for use in multicellular tumour spheroids (MTS) – a three dimensional (3D) cell model which better mimics the tumour environment and gradients that develop. MTS are a more realistic model of the in vivo cellular morphology and environment and are becoming an increasingly popular in vitro model, replacing traditional monolayer culture. Imaging techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM) and helium ion microscopy (HIM) were used to investigate differences in morphology and NS uptake in monolayer culture compared to MTS. After confirming NS uptake, the first SERS measurements revealing quantitative information on redox potential in MTS were performed.
{"title":"Monolayer to MTS: using SEM, HIM, TEM and SERS to compare morphology, nanosensor uptake and redox potential in MCF7 cells","authors":"Lauren E. Jamieson, A. P. Bell, David J. Harrison, Colin Campbell","doi":"10.1117/12.2180944","DOIUrl":"https://doi.org/10.1117/12.2180944","url":null,"abstract":"Cellular redox potential is important for the control and regulation of a vast number of processes occurring in cells. When the fine redox potential balance within cells is disturbed it can have serious consequences such as the initiation or progression of disease. It is thought that a redox gradient develops in cancer tumours where the peripheral regions are well oxygenated and internal regions, further from vascular blood supply, become starved of oxygen and hypoxic. This makes treatment of these areas more challenging as, for example, radiotherapy relies on the presence of oxygen. Currently techniques for quantitative analysis of redox gradients are limited. Surface enhanced Raman scattering (SERS) nanosensors (NS) have been used to detect redox potential in a quantitative manner in monolayer cultured cells with many advantages over other techniques. This technique has considerable potential for use in multicellular tumour spheroids (MTS) – a three dimensional (3D) cell model which better mimics the tumour environment and gradients that develop. MTS are a more realistic model of the in vivo cellular morphology and environment and are becoming an increasingly popular in vitro model, replacing traditional monolayer culture. Imaging techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM) and helium ion microscopy (HIM) were used to investigate differences in morphology and NS uptake in monolayer culture compared to MTS. After confirming NS uptake, the first SERS measurements revealing quantitative information on redox potential in MTS were performed.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124019503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Broadway, D. Gallego, G. Woyessa, A. Pospori, G. Carpintero, O. Bang, K. Sugden, H. Lamela
Opto-Acoustic Endoscopy (OAE) requires sensors with a high sensitivity and small physical dimensions in order to facilitate integration into an endoscope of less than 1mm in diameter. We present fibre Bragg grating (FBG) and Fabry- Perot intrinsic fibre sensors for ultrasound detection. We present a structure profile characterisation setup to analyse tune the fibre sensors in preparation for ultrasonic detection. We evaluate the suitability of the different structures and grating parameters for ultrasonic sensing. By analysing the prepared gratings, we enable the optimisation of the profile and a simplification of the detection regime for an optimal interferometric OAE configuration.
{"title":"Fabry-Perot micro-structured polymer optical fibre sensors for opto-acoustic endoscopy","authors":"C. Broadway, D. Gallego, G. Woyessa, A. Pospori, G. Carpintero, O. Bang, K. Sugden, H. Lamela","doi":"10.1117/12.2181095","DOIUrl":"https://doi.org/10.1117/12.2181095","url":null,"abstract":"Opto-Acoustic Endoscopy (OAE) requires sensors with a high sensitivity and small physical dimensions in order to facilitate integration into an endoscope of less than 1mm in diameter. We present fibre Bragg grating (FBG) and Fabry- Perot intrinsic fibre sensors for ultrasound detection. We present a structure profile characterisation setup to analyse tune the fibre sensors in preparation for ultrasonic detection. We evaluate the suitability of the different structures and grating parameters for ultrasonic sensing. By analysing the prepared gratings, we enable the optimisation of the profile and a simplification of the detection regime for an optimal interferometric OAE configuration.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131370655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. D. O. Gonçalves, Mônica N. da Silva, Lilia Coronato Courrol
The atheromatous plaques exhibit an accumulation of protoporphyrin IX, or PpIX, which is transferred to the feces. In this work it was associated a precursor of the PpIX, the 5-aminolevulinic acid, ALA, with gold nanoparticles (ALA:AuNps). The objective was verify the possibility to use ALA:AuNPs as a diagnosis agent for atherosclerosis. ALA:AuNps were synthesized mixing ALA with Tetrachloroauric(III) acid in miliQ water solution followed by photoreduction with light from a Xenon lamp. A total of 22 Male New Zealand rabbits were divided into 3 groups: control group (CG) where animals received normal diet, control group with ALA (CGALA ) and Experimental Group with ALA:AuNPs ( EGALAAu ) in which the animals received a diet with 1% cholesterol. Measurements of the emission intensity of extracted porphyrins from the feces in the region between 575 and 725 nm were done. An increase in the feces porphyrin emission after ALA and ALA:AuNPs administration was observed.
{"title":"Identification of atherosclerosis using aminolevulinic gold nanoparticle assay in fecal specimens","authors":"K. D. O. Gonçalves, Mônica N. da Silva, Lilia Coronato Courrol","doi":"10.1117/12.2180916","DOIUrl":"https://doi.org/10.1117/12.2180916","url":null,"abstract":"The atheromatous plaques exhibit an accumulation of protoporphyrin IX, or PpIX, which is transferred to the feces. In this work it was associated a precursor of the PpIX, the 5-aminolevulinic acid, ALA, with gold nanoparticles (ALA:AuNps). The objective was verify the possibility to use ALA:AuNPs as a diagnosis agent for atherosclerosis. ALA:AuNps were synthesized mixing ALA with Tetrachloroauric(III) acid in miliQ water solution followed by photoreduction with light from a Xenon lamp. A total of 22 Male New Zealand rabbits were divided into 3 groups: control group (CG) where animals received normal diet, control group with ALA (CGALA ) and Experimental Group with ALA:AuNPs ( EGALAAu ) in which the animals received a diet with 1% cholesterol. Measurements of the emission intensity of extracted porphyrins from the feces in the region between 575 and 725 nm were done. An increase in the feces porphyrin emission after ALA and ALA:AuNPs administration was observed.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133579563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Leggio, O. de Varona, P. Escudero, Guillermo Carpintero Del Barrio, M. Osiński, Horacio Lamela Rivera
During the last decade, Optoacoustic Imaging (OAI), or Optoacoustic Tomography (OAT), has evolved as a novel imaging technique based on the generation of ultrasound waves with laser light. OAI may become a valid alternative to techniques currently used for the detection of diseases at their early stages. It has been shown that OAI combines the high contrast of optical imaging techniques with high spatial resolution of ultrasound systems in deep tissues. In this way, the use of nontoxic biodegradable contrast agents that mark the presence of diseases in near-infrared (NIR) wavelengths range (0.75–1.4 um) has been considered. The presence of carcinomas and harmful microorganisms can be revealed by means of the fluorescence effect exhibited by biopolymer nanoparticles. A different approach is to use carbon nanotubes (CNTs) which are a contrast agent in NIR range due to their absorption characteristics in the range between 800 to 1200 nm. We report a multi-wavelength (870 and 905 nm) laser diode–based optoacoustic (OA) system generating ultrasound signals from a double-walled carbon nanotubes (DWCNTs) solution arranged inside a tissue-like phantom, mimicking the scattering of a biological soft tissue. Optoacoustic signals obtained with DWCNTs inclusions within a tissue-like phantom are compared with the case of ink-filled inclusions, with the aim to assess their absorption. These measurements are done at both 870 and 905 nm, by using high power laser diodes as light sources. The results show that the absorption is relatively high when the inclusion is filled with ink and appreciable with DWCNTs.
{"title":"A compact multi-wavelength optoacoustic system based on high-power diode lasers for characterization of double-walled carbon nanotubes (DWCNTs) for biomedical applications","authors":"L. Leggio, O. de Varona, P. Escudero, Guillermo Carpintero Del Barrio, M. Osiński, Horacio Lamela Rivera","doi":"10.1117/12.2181021","DOIUrl":"https://doi.org/10.1117/12.2181021","url":null,"abstract":"During the last decade, Optoacoustic Imaging (OAI), or Optoacoustic Tomography (OAT), has evolved as a novel imaging technique based on the generation of ultrasound waves with laser light. OAI may become a valid alternative to techniques currently used for the detection of diseases at their early stages. It has been shown that OAI combines the high contrast of optical imaging techniques with high spatial resolution of ultrasound systems in deep tissues. In this way, the use of nontoxic biodegradable contrast agents that mark the presence of diseases in near-infrared (NIR) wavelengths range (0.75–1.4 um) has been considered. The presence of carcinomas and harmful microorganisms can be revealed by means of the fluorescence effect exhibited by biopolymer nanoparticles. A different approach is to use carbon nanotubes (CNTs) which are a contrast agent in NIR range due to their absorption characteristics in the range between 800 to 1200 nm. We report a multi-wavelength (870 and 905 nm) laser diode–based optoacoustic (OA) system generating ultrasound signals from a double-walled carbon nanotubes (DWCNTs) solution arranged inside a tissue-like phantom, mimicking the scattering of a biological soft tissue. Optoacoustic signals obtained with DWCNTs inclusions within a tissue-like phantom are compared with the case of ink-filled inclusions, with the aim to assess their absorption. These measurements are done at both 870 and 905 nm, by using high power laser diodes as light sources. The results show that the absorption is relatively high when the inclusion is filled with ink and appreciable with DWCNTs.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116159153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Red blood cell aggregation is one of the most important factors in blood viscosity at stasis or at very low rates of flow. The basic structure of aggregates is a linear array of cell commonly termed as rouleaux. Enhanced or abnormal aggregation is seen in clinical conditions, such as diabetes and hypertension, producing alterations in the microcirculation, some of which can be analyzed through the characterization of aggregated cells. Frequently, image processing and analysis for the characterization of RBC aggregation were done manually or semi-automatically using interactive tools. We propose a system that processes images of RBC aggregation and automatically obtains the characterization and quantification of the different types of RBC aggregates. Present technique could be interesting to perform the adaptation as a routine used in hemorheological and Clinical Biochemistry Laboratories because this automatic method is rapid, efficient and economical, and at the same time independent of the user performing the analysis (repeatability of the analysis).
{"title":"Automatic analysis of microscopic images of red blood cell aggregates","authors":"P. Menichini, Mónica G. Larese, B. Riquelme","doi":"10.1117/12.2181110","DOIUrl":"https://doi.org/10.1117/12.2181110","url":null,"abstract":"Red blood cell aggregation is one of the most important factors in blood viscosity at stasis or at very low rates of flow. The basic structure of aggregates is a linear array of cell commonly termed as rouleaux. Enhanced or abnormal aggregation is seen in clinical conditions, such as diabetes and hypertension, producing alterations in the microcirculation, some of which can be analyzed through the characterization of aggregated cells. Frequently, image processing and analysis for the characterization of RBC aggregation were done manually or semi-automatically using interactive tools. We propose a system that processes images of RBC aggregation and automatically obtains the characterization and quantification of the different types of RBC aggregates. Present technique could be interesting to perform the adaptation as a routine used in hemorheological and Clinical Biochemistry Laboratories because this automatic method is rapid, efficient and economical, and at the same time independent of the user performing the analysis (repeatability of the analysis).","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"123 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121928418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. C. Fortunato, C. Kurachi, V. Bagnato, L. Moriyama
The use of light as a therapeutic agent has been the subject of several studies; however, the dosimetry for its clinical application is still based on empirical data. The propagation of light in biological tissues depends on the tissue optical properties, and these properties may vary among people, tissues and sites, making it diffcult to establish dosimetry. In this context, the research for methods to determine the spatial distribution of light in individual biological tissues becomes essential, allowing the individual dosimetry. This study aims to image the diffuse reflectance at the optical phantom surface to infer the spatial distribution of light inside a phantom when an absorbing obstacle is present. Red laser were used as light source on solid turbid optical phantom; a small black sphere was used as absorbing obstacle. It is important to know, in real time and in a non-invasive way, about the existence of heterogeneities that may compromise the light propagation inside a biological tissue, so that the light dosimetry might be properly established.
{"title":"Diffuse reflectance imaging to predict heterogeneities in turbid optical phantom","authors":"T. C. Fortunato, C. Kurachi, V. Bagnato, L. Moriyama","doi":"10.1117/12.2180985","DOIUrl":"https://doi.org/10.1117/12.2180985","url":null,"abstract":"The use of light as a therapeutic agent has been the subject of several studies; however, the dosimetry for its clinical application is still based on empirical data. The propagation of light in biological tissues depends on the tissue optical properties, and these properties may vary among people, tissues and sites, making it diffcult to establish dosimetry. In this context, the research for methods to determine the spatial distribution of light in individual biological tissues becomes essential, allowing the individual dosimetry. This study aims to image the diffuse reflectance at the optical phantom surface to infer the spatial distribution of light inside a phantom when an absorbing obstacle is present. Red laser were used as light source on solid turbid optical phantom; a small black sphere was used as absorbing obstacle. It is important to know, in real time and in a non-invasive way, about the existence of heterogeneities that may compromise the light propagation inside a biological tissue, so that the light dosimetry might be properly established.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127282002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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