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Review of Coconut “Lethal Yellowing” type diseases Diversity, variability and diagnosis 椰子“致黄”型疾病多样性、变异性及诊断研究进展
Pub Date : 2009-03-01 DOI: 10.1051/OCL.2009.0246
M. Dollet, R. Quaicoe, F. Pilet
Coconut palms (Cocos nucifera L.) can be affected by several types of Lethal Yellowing (LY) diseases worldwide. Some of the syndromes are caused by phytoplasmas, small bacteria that are impossible to detect by light microscopy. Amplification of a given gene of the phytoplasmas by polymerase chain reaction (PCR) is the most convenient diagnosis method. The problem is that there are at least 28 “groups” of phytoplasmas and only one pair of primers -P1/P7- commonly used for PCR. As these primers belong to a very conserved gene, false positives are frequent. Consequently, alternative primers specific to one “strain” (or subgroup) have to be used, such as LY-F/LY-R for the Caribbean LY, Rohde primers for LD Tanzania. Such specific primers are sometimes restrictive. Indeed, there is variability within each strain and the sequence of the primers has to be adapted to that variability. There are at least five LY subgroups. The subgroups can only be identified by restriction fragment length polymorphism or sequencing. In Africa, two subgroups of LY phytoplasmas have been identified so far.
在世界范围内,椰子树(Cocos nucifera L.)可受到几种致命黄化(LY)疾病的影响。有些综合征是由植物原体引起的,植物原体是一种不可能通过光学显微镜检测到的小细菌。用聚合酶链反应(PCR)扩增植物原体的特定基因是最方便的诊断方法。问题是至少有28个植物原体“群”,而通常用于PCR的引物只有一对——p1 /P7。由于这些引物属于非常保守的基因,因此经常出现假阳性。因此,必须使用针对一个“菌株”(或亚群)的替代引物,例如针对加勒比LY的LY- f /LY- r引物,针对坦桑尼亚LD的Rohde引物。这种特定的引物有时是限制性的。事实上,每个菌株都有变异,引物序列必须适应这种变异。至少有五个LY子组。亚群只能通过限制性片段长度多态性或测序来识别。在非洲,迄今已确定了LY植物原体的两个亚群。
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引用次数: 26
Sécurité alimentaire et mondialisation 粮食安全和全球化
Pub Date : 2008-03-01 DOI: 10.1051/OCL.2008.0179
M. Griffon
The world food problem becomes more and more a problem of: - Economic and social equity on a global scale. - Environmental policy because the future of the biosphere through the future land is at stake; - Trade policy: it is hard to imagine that structural adjustment policies adverse to agriculture and trade regime of WTO can persist in this context, including the acceptance of an environmental dumping and social dumping on the part of Agriculture Latin American issues that will come on the agenda of WTO negotiations. - Agricultural policy because it is not clear how great productive effort that is necessary may be possible without an accompanying agricultural policy. - Technological invention because it must combine productivity and ecology. - Education on the one hand to get a large portion of farmers in this poor world to new technologies, and secondly to ensure there is anywhere in the world a true education to food.
世界粮食问题越来越成为一个全球范围内的经济和社会公平问题。-环境政策,因为通过未来土地的生物圈的未来受到威胁;-贸易政策:很难想象不利于农业和世贸组织贸易制度的结构调整政策能够在这种情况下持续下去,包括接受农业方面的环境倾销和社会倾销。拉丁美洲问题将列入世贸组织谈判议程。-农业政策,因为不清楚如果没有配套的农业政策,需要多大的生产努力是可能的。-技术发明,因为它必须结合生产力和生态。教育一方面是为了让这个贫穷世界的大部分农民掌握新技术,其次是为了确保世界上任何地方都有真正的食物教育。
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引用次数: 0
L’inclusion des normes sociales à l’OMC : vrai leurre, fausse panacée ? 将社会标准纳入世贸组织:虚假的欺骗,虚假的灵丹妙药?
Pub Date : 2007-09-01 DOI: 10.1051/ocl.2007.0138
T. Voituriez
Labour cost gaps between developed and emerging countries justify in political discourses in France the implementation of policy measures such as tariff barriers, social VAT, and/or the inclusion of Social Clause in the WTO to protect developed economies against allegedly "dumped" imports from developing countries. We examine each of these policy measures to show that their contribution to tackling the wage gap issue between rich and poor countries would be marginal and much below politicians claims and opinion expectations. (Resume d'auteur)
在法国的政治话语中,发达国家和新兴国家之间的劳动力成本差距证明了实施关税壁垒、社会增值税和/或在世贸组织中纳入社会条款等政策措施的合理性,以保护发达经济体免受来自发展中国家的所谓“倾销”进口。我们研究了每一项政策措施,以表明它们对解决富国和穷国之间工资差距问题的贡献将是微不足道的,远低于政治家的主张和舆论预期。(简历d 'auteur)
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引用次数: 0
Adaptation à la sécheresse et création variétale : le cas de l’arachide en zone sahélienne - Deuxième partie : une approche pluridisciplinaire pour la création variétale 干旱适应和品种创造:以萨赫勒地区的花生为例-第二部分:品种创造的多学科方法
Pub Date : 2007-09-01 DOI: 10.1051/OCL.2007.0141
D. Clavel, P. Baradat, J. Khalfaoui, N. Drame, N. Diop, O. Diouf, Y. Zuily-Fodil
Drought is a multiform constraint whose impact on the vegetal metabolism is very variable according to its duration, intensity and phenological stage of the vegetal development where it occurs. Thus, the plant resistance is expressed at different plant organisation levels. The present study was aimed at integrating knowledge generated by experiments carried out in Senegal on groundnut within the framework of a breeding programme geared towards improving groundnut yield under drought conditions. Three studies involved in the breeding work, are presented. The first chapter analyses an incomplete half-diallel cross performed on an original population under recurrent selection for drought adaptation. The study confirmed the weak heritability of yields but concludes that the best predictor of pod-yield was the pod-yield itself. By contrast, the study of the genetic correlations showed that a selection for high haulm-yield could lead to poor pod-maturity under drought constraint. The selection indices were performed and used to estimate genetic gains relative to the main agronomic characters according to selection pressure. The second chapter covers the genetic variability of phenological, agronomic and physiological characters studied in two series of quasi-isogenic early lines. It has indicated that genetic variability was expressed in these lines despite its closeness. Some correlations between yield and physiological parameters, i.e. mainly fluorescence parameters, were significant but not stable across lines and environments showing that groundnut have different drought adaptation strategies according to genetic background and drought pattern. This work was pursued at the molecular level with three reference cvs involving the both recurrent parents of the precedent study. The gene transcript kinetics under drought, obtained using RT-PCR, showed that Phospolipase D and Cysteine protease gene expressions were stimulated by stress in the most susceptible cultivars, whereas their was higher LEA gene expression in the resistant one. These interconnected experiments conducted at different plant organisation levels led to the development of a general methodological model and of new improved genotypes to meet the social demand.
干旱是一种多形式的约束,其对植物代谢的影响因其发生的持续时间、强度和物候阶段而异。因此,植物抗性是在不同的植物组织水平上表达的。本研究的目的是在一个旨在提高干旱条件下花生产量的育种方案的框架内,把在塞内加尔进行的花生试验所产生的知识综合起来。介绍了在育种工作中所涉及的三项研究。第一章分析了一种不完全半双列杂交对一个原始种群在周期性选择下适应干旱的影响。该研究证实了产量的弱遗传力,但得出结论,籽粒产量的最佳预测因子是籽粒产量本身。相比之下,遗传相关研究表明,在干旱约束下,高产的选择可能导致籽粒成熟度较低。根据不同的选择压力,编制了不同的选择指数,并利用这些选择指数来估计相对于主要农艺性状的遗传增益。第二章对两个准等基因早期系物候、农艺和生理性状的遗传变异进行了研究。结果表明,尽管亲缘关系密切,但遗传变异在这些系中仍有表达。花生产量与生理参数(主要是荧光参数)之间存在显著的相关性,但不稳定,这表明花生在不同的遗传背景和干旱模式下具有不同的干旱适应策略。这项工作是在分子水平上进行的,涉及先例研究的两个复发父母的三个参考cvs。利用RT-PCR获得的干旱条件下基因转录动力学结果表明,在干旱条件下,大多数易感品种的磷脂酶D和半胱氨酸蛋白酶基因表达受到刺激,而在抗性品种中,它们的LEA基因表达较高。这些在不同植物组织水平上进行的相互关联的实验导致了一种通用方法模型和新的改良基因型的发展,以满足社会需求。
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引用次数: 3
Cycle du développement et PMA : les pays les plus pauvres bénéficieront-ils de la libéralisation des échanges ? 发展回合和最不发达国家:最贫穷国家会从贸易自由化中受益吗?
Pub Date : 2006-07-01 DOI: 10.1051/OCL.2006.0039
F. Gérard
The current trade negotiations' round has been called the development round in order to emphasize ambitious objectives in terms of poverty alleviation: halving the number of poor by 2015. However estimates from world economic models show little improvement for the Less Developed Countries. Moreover several models assumptions may overestimate gains and underestimate losses, especially in the poorest countries where numerous markets imperfections hold. It seems then necessary to avoid that poverty reduction be restricted to emerging countries that development policies and their funding in LDC be discussed simultaneously to trade negotiations. (Resume d'auteur)
目前的贸易谈判回合被称为发展回合,以强调在减轻贫困方面的雄心勃勃的目标:到2015年将贫困人口数量减半。然而,根据世界经济模型的估计,欠发达国家的情况几乎没有改善。此外,一些模型的假设可能高估了收益,低估了损失,特别是在许多市场不完善的最贫穷国家。因此,似乎有必要避免将减贫局限于新兴国家,在贸易谈判中同时讨论最不发达国家的发展政策及其筹资问题。(简历d 'auteur)
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引用次数: 0
Towards the revision of the EU biofuels directive what future for european biodiesel markets 对于欧盟生物燃料指令的修订,欧洲生物柴油市场的未来是什么
Pub Date : 2006-03-01 DOI: 10.1051/OCL.2006.0032
Raffaelo Garofalo
The European Commission issued its first “Communication on alternative fuels for road transportation and on a set of measures to promote the use of biofuels” in November 2001 [1]. As from its beginnings the EU biofuels strategy was conceived as a visionary policy, based on the assumption that biofuels promotion was needed at least for three main reasons: contribute to the security and independence of energy supply, reduce GHG (Greenhouse emissions Gaz) and CO2 emissions from the transport sector, create an additional outlet for EU agricultural production thus encouraging rural development. Since then facts have confirmed that the EU biofuels strategy was indeed a visionary policy, conceived at an early stage to tackle problems whose solution has become more and more urgent across the last years.
2001年11月,欧盟委员会发布了第一份《关于道路运输替代燃料和促进生物燃料使用的一系列措施的通讯》[1]。从一开始,欧盟生物燃料战略就被认为是一项有远见的政策,基于生物燃料推广至少有三个主要原因的假设:促进能源供应的安全和独立,减少运输部门的温室气体(温室气体排放)和二氧化碳排放,为欧盟农业生产创造额外的出口,从而鼓励农村发展。从那时起,事实证实,欧盟生物燃料战略确实是一项有远见的政策,在早期阶段就构思出来,旨在解决过去几年来日益紧迫的问题。
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引用次数: 1
Adaptation à la sécheresse et création variétale : le cas de l'arachide en zone sahélienne Première partie : revue bibliographique 干旱适应和品种创造:以萨赫勒地区花生为例第一部分:文献综述
Pub Date : 2005-05-01 DOI: 10.1051/OCL.2005.0248
D. Clavel, N. Drame, N. Diop, Y. Zuily-Fodil
Drought is a multiform constraint expressing at different plant organisation levels. It is recognised as the first factor limiting the agriculture production in the world. The Sahel subtropical regions were the most exposed to the devasting effects of the recent climate change. A state of knowledge referring to the plant responses to drought is a fundamental initial step to any program of selection. This review concerns more particularly groundnut (Arachis hypogaea L.), which is a very common legume in the Sahel areas. Critical evaluations of traits as relevant selection criteria for a breeding program aiming at improving yield and yield stability under drought constraint were made. The genetic specificities of the species are exposed in the first part. Then a detailed description of the grain-legume traits related to drought adaptation is presented. The review was widened to other plants for the molecular responses to water deficit, supposed to be more general. Finally, the last part supplied an updated synthesis of the recent advances in biotechnology on groundnut with special focus on drought tolerance.
干旱是一种多形式的约束,表现在不同的植物组织水平上。它被认为是世界上限制农业生产的第一因素。萨赫勒亚热带地区最容易受到最近气候变化的破坏性影响。了解植物对干旱的反应是任何选择程序的基本初始步骤。本综述特别关注花生(Arachis hypogaea L.),这是萨赫勒地区非常常见的豆科植物。在干旱条件下,以提高产量和稳定产量为目标的育种计划中,对性状进行了关键性评价,作为相关的选择标准。第一部分揭示了该物种的遗传特性。然后详细介绍了与干旱适应有关的籽粒豆科植物性状。对其他植物对水分缺乏的分子反应进行了综述,认为这是更为普遍的。最后,最后一部分提供了花生生物技术最新进展的最新综合,特别侧重于耐旱性。
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引用次数: 7
Roundtable on Sustainable Palm Oil - RSPO. The second RSPO meeting in Jakarta in October 2004. 可持续棕榈油圆桌会议- RSPO。2004年10月在雅加达举行的第二次RSPO会议。
Pub Date : 2005-03-01 DOI: 10.1051/OCL.2005.0125
H. Omont
In a context of discord between producers and environmental conservationists, RSPO is an initiative by stakeholders in the “Oil Palm” commodity chain to promote sustainable palm oil production. RSPO, using a multiple-stakeholder process, based its approach on drawing up a set of credible criteria that define the sustainability of palm oil production and which are acceptable to the different categories of stakeholders. The purpose of the second meeting (RT2) was to: i) propose a platform for exchanging views and experience between stakeholders from industrialized and emergent countries, to seek a clear definition of the “sustainable palm oil” concept; ii) identify practical projects for facilitating the implementation of good practices and for proceeding with their introduction; and: iii) strengthen cooperation and mutual assistance between stakeholders and international agencies to promote the production and use of “sustainable palm oil”.
在生产者和环保主义者之间不和的背景下,RSPO是“油棕”商品链上的利益相关者为促进可持续棕榈油生产而发起的一项倡议。RSPO采用多利益相关者流程,其方法基于起草一套可信的标准,这些标准定义了棕榈油生产的可持续性,并为不同类别的利益相关者所接受。第二次会议(RT2)的目的是:i)为来自工业化国家和新兴国家的利益相关者提供一个交流意见和经验的平台,以寻求对“可持续棕榈油”概念的明确定义;Ii)确定切实可行的项目,以促进良好做法的实施和推行;加强利益相关者和国际机构之间的合作与互助,促进“可持续棕榈油”的生产和使用。
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引用次数: 12
Carbon storage and global change: the role of oil palm 碳储存与全球变化:油棕的作用
Pub Date : 2005-03-01 DOI: 10.1051/OCL.2005.0154
E. Lamade, J. Bouillet
In the context of global change, potential estimations of carbon storage by the oil palm ecosystem in different ecologies have been calculated for the major productive countries in Africa, Asian and American continents. Comparisons were done with other types of planted ecosystems as eucalyptus and coconut as well as different types of natural forests. Carbon budget components as NPP, autotrophic and heterotrophic soil respiration, litter and fine litter contributions were discussed in regards to the very high rate of carbon sequestration by oil palm ecosystem : from 250 to 940 C m–2 yr –1 (estimations including harvested bunches).
在全球变化的背景下,对非洲、亚洲和美洲大陆的主要生产国进行了不同生态系统中油棕生态系统碳储量的潜在估算。与桉树和椰子等其他类型的种植生态系统以及不同类型的天然林进行了比较。针对油棕生态系统非常高的碳固存率(从250到940 C m-2 yr -1),讨论了碳收支组分,如NPP、自养和异养土壤呼吸、凋落物和细凋落物的贡献(包括收获束的估计)。
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引用次数: 67
Oil palm seed distribution 油棕种子分布
Pub Date : 2005-03-01 DOI: 10.1051/OCL.2005.0148
T. Durand-Gasselin, B. Cochard
For a tropical plant, the oil palm commodity chain has the peculiarity of possessing a major seed production sector for reasons that are primarily genetic. This seed sector has numerous original aspects. Breeders are also propagators and usually also distribute their seeds. Oil palm seeds are semi-recalcitrant: they display pseudo-dormancy. Achieving seed germination is difficult and requires lengthy treatments and special installations. This restriction greatly influences seed distribution and the role of the different stakeholders in the commodity chain. It was only once it had been discovered how the "sh" gene functioned, which controls shell thickness, and when it became necessary to produce "tenera" seeds derived from exclusively "dura x pisifera" crosses, that a true seed market developed. In addition it is difficult to organize seed distribution to smallholders. This is partly due to difficulties that the profession, or a State-run organization, has in controlling middlemen networks, and partly to the absence of any protective systems (UPOV, plant breeder certificate, etc.) that generally oblige breeders to preserve and propagate parents in their own installations. In fact there are major inequalities in the access to seeds between agroindustry and smallholders. Another peculiarity of the oil palm seed market is the virtually total absence of guarantees for buyers: the quality of the research conducted by breeders, the seed production strategies necessary for transferring genetic progress, and the technical quality of production. The only guarantee today comes from the relations of confidence established year after year between breeders/distributors and growers. In this fields, research can lead to some proposals: molecular biology offers some interesting prospects for certifying seed quality and social science develop effective communication methods.
作为一种热带植物,油棕商品链的特点是拥有一个主要的种子生产部门,主要原因是遗传。这个种子部门有许多原创性的方面。育种者也是传播者,通常也传播他们的种子。油棕种子是半顽固性的:它们表现出伪休眠状态。实现种子发芽是困难的,需要长时间的处理和特殊的装置。这一限制极大地影响了种子的分配和商品链中不同利益相关者的作用。只有当人们发现了控制壳厚的“sh”基因是如何起作用的,并且有必要生产完全由“硬脑膜与pisifera”杂交而来的“tenera”种子时,真正的种子市场才得以发展。此外,很难组织向小农分发种子。这部分是由于该行业或国营组织在控制中间商网络方面存在困难,部分是由于缺乏任何保护制度(UPOV,植物育种者证书等),这些制度通常迫使育种者在自己的设施中保存和繁殖亲本。事实上,在获得种子方面,农用工业和小农之间存在着很大的不平等。油棕种子市场的另一个特点是几乎完全没有对买家的保证:育种者进行的研究质量、转移遗传进展所需的种子生产策略以及生产的技术质量。今天唯一的保证来自育种者/分销商和种植者之间年复一年建立的信任关系。在这一领域,研究可以带来一些建议:分子生物学为种子质量认证提供了一些有趣的前景,社会科学开发了有效的交流方法。
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引用次数: 9
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Oleagineux Corps gras Lipides
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