Pub Date : 2018-11-28DOI: 10.20884/1.OA.2018.14.3.584
A. D. Syakti
This critical review highlighted the need to booster an application of the environment biotechnology in order to reduce petroleum hydrocarbons contamination in marine environment. Marine bioremediation can be promisingly applied when the spills occurred. However, absence of policy support from Indonesian government to endorse the private actor’s liabilities when complying the rehabilitation action was no significance while academic research was still far from establishment of fundamental knowledge in marine bioremediation. Thus, if so, it very likely that bioremediation disciplines and application might “die before blossom” in a country that often confronted by the maritime risk contamination of petroleum hydrocarbons on their marine and coastal area.
{"title":"Marine Bioremediation in Indonesia : Die Before Blossom","authors":"A. D. Syakti","doi":"10.20884/1.OA.2018.14.3.584","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.3.584","url":null,"abstract":"This critical review highlighted the need to booster an application of the environment biotechnology in order to reduce petroleum hydrocarbons contamination in marine environment. Marine bioremediation can be promisingly applied when the spills occurred. However, absence of policy support from Indonesian government to endorse the private actor’s liabilities when complying the rehabilitation action was no significance while academic research was still far from establishment of fundamental knowledge in marine bioremediation. Thus, if so, it very likely that bioremediation disciplines and application might “die before blossom” in a country that often confronted by the maritime risk contamination of petroleum hydrocarbons on their marine and coastal area.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":"32 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41269779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-28DOI: 10.20884/1.OA.2018.14.3.494
Saga Gerlaping Negari, E. N. Dewi, L. Rianingsih
Bekasam is one of fermentation products made from Milkfish (Chanos chanos Forsk). The aim of this study was to determine the effect of different sources of carbohydrates on fermented waste to fatty acid composition. The method used was experimental laboratories using Completely Randomized Design. The treatment applied was the use of different carbohydrate sources of rice, brown sugar and garlic in the milkfish bekasam production using 3 repetitions. The data were analyzed using Analysis of Variance and continued by Honestly Significant Difference test if there was any effect on the treatment given. The results showed that the difference of carbohydrate sources during fermentation process could affect the composition of fatty acids. Lactic acid bacteria use carbohydrate sources as energy to grow and then hydrolyze fat into fatty acids. The best result was bekasam with the sources of carbohydrate of rice with total value of Lactic Acid Bacteria 7,888 log cfu/g, Total Volatile Base Nitrogen value 34,796 mgN/100ml, lipid content 0,055% and the highest fatty acids compositions. The results of the study showed that the use of different sources of carbohydrate on bekasam had significant effect (P<5%) on the fatty acid composition on bekasam.
{"title":"Change of Fatty Acids Compositions (Omega 3, 6, 9) from Milkfish (Chanos chanos Forsk) Bekasam Fermented with Different Carbohydrates Sources","authors":"Saga Gerlaping Negari, E. N. Dewi, L. Rianingsih","doi":"10.20884/1.OA.2018.14.3.494","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.3.494","url":null,"abstract":"Bekasam is one of fermentation products made from Milkfish (Chanos chanos Forsk). The aim of this study was to determine the effect of different sources of carbohydrates on fermented waste to fatty acid composition. The method used was experimental laboratories using Completely Randomized Design. The treatment applied was the use of different carbohydrate sources of rice, brown sugar and garlic in the milkfish bekasam production using 3 repetitions. The data were analyzed using Analysis of Variance and continued by Honestly Significant Difference test if there was any effect on the treatment given. The results showed that the difference of carbohydrate sources during fermentation process could affect the composition of fatty acids. Lactic acid bacteria use carbohydrate sources as energy to grow and then hydrolyze fat into fatty acids. The best result was bekasam with the sources of carbohydrate of rice with total value of Lactic Acid Bacteria 7,888 log cfu/g, Total Volatile Base Nitrogen value 34,796 mgN/100ml, lipid content 0,055% and the highest fatty acids compositions. The results of the study showed that the use of different sources of carbohydrate on bekasam had significant effect (P<5%) on the fatty acid composition on bekasam.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49184296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-10DOI: 10.20884/1.OA.2018.14.2.570
D. Rachmawati, I. Samidjan
This study aims to examine the effect of phytase enzyme in the diet on digestibility of growth of white shrimp (Litopenaeus vannamei) raised in intensive system culture. Research on the suplementation of phytase enzymes in L. vannamei diet is very important to hydrolyze phytic acid in vegetable ingredients as a source of diet protein so as to increase the efficiency of diet utilization to support growth of vaname shrimp. The shrimp used in the research had average weight of 1,19±0,06 g/shrimp. The diet contained 38% of protein and 3200 Kcal DE/kg with various doses of phytase enzyme suplementation, namely: A (0 U/kg diet), B (500 U/kg diet), C (1000 U/kg diet) and D (1500 U/kg diet). Observed parameters were digestibility of phosphore (ADCF), disgetibility of protein (ADCP), efficiency of diet utilitzation (EFU), protein efficiency ratio (PER), diet convesion ratio (FCR) relative growth rate (RGR), and survival rate (SR). The suplementation of phytase enzyme in the diet significantly influenced (P<0.01) on ADCF, ADCP, EFU, PER, FCR, RGR; however, it did not significantly influence on SR of white shrimp. The best treatment result was the suplementation of 1000 U/kg diet phytase enzyme (treatment C). The optimum dose of phytase enzyme in the diet was 867, 920, 923, 915, 1000 and 1000 U/kg of diet respectively for ADCF, ADCP, EFU, PER, FCR, RGR. Based on those result the suplementation of phytase enzyme can increase the growth of white shrimp.
{"title":"Engineering Technology of White Shrimp (Litopenaeus vannamei) Intensive System Culture with the Suplementation of Phytase Enzyme in the Diet","authors":"D. Rachmawati, I. Samidjan","doi":"10.20884/1.OA.2018.14.2.570","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.2.570","url":null,"abstract":"This study aims to examine the effect of phytase enzyme in the diet on digestibility of growth of white shrimp (Litopenaeus vannamei) raised in intensive system culture. Research on the suplementation of phytase enzymes in L. vannamei diet is very important to hydrolyze phytic acid in vegetable ingredients as a source of diet protein so as to increase the efficiency of diet utilization to support growth of vaname shrimp. The shrimp used in the research had average weight of 1,19±0,06 g/shrimp. The diet contained 38% of protein and 3200 Kcal DE/kg with various doses of phytase enzyme suplementation, namely: A (0 U/kg diet), B (500 U/kg diet), C (1000 U/kg diet) and D (1500 U/kg diet). Observed parameters were digestibility of phosphore (ADCF), disgetibility of protein (ADCP), efficiency of diet utilitzation (EFU), protein efficiency ratio (PER), diet convesion ratio (FCR) relative growth rate (RGR), and survival rate (SR). The suplementation of phytase enzyme in the diet significantly influenced (P<0.01) on ADCF, ADCP, EFU, PER, FCR, RGR; however, it did not significantly influence on SR of white shrimp. The best treatment result was the suplementation of 1000 U/kg diet phytase enzyme (treatment C). The optimum dose of phytase enzyme in the diet was 867, 920, 923, 915, 1000 and 1000 U/kg of diet respectively for ADCF, ADCP, EFU, PER, FCR, RGR. Based on those result the suplementation of phytase enzyme can increase the growth of white shrimp.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46377084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-10DOI: 10.20884/1.oa.2018.14.2.571
I. Samidjan, D. Rachmawati
Objectives for snakeheads farming Cngineered to produce superior products based artificial diet eenrichment vitamin C with different doses and the use of microsatellites to increase growth and determine the superior quality. Methods February to November 2017 Examined the Effect of artificial feed Enriched with vitamin C doses per 100 g feed were given 0 mg (T1), 12 mg (T2), 24 mg ,and 36 mg (T3), 48 mg (T4) were using a completely randomized design (CRD) and 4 treatments and 3 replications. Furthermore, to determine the superior quality using microsatellite genetic markers to see the genetic code snakeheads. Then the treatment was analyzed by ANOVA and data arrangement of molecular weight DNA, polymorphism were analyzed by Cco-stat and Minitab version 11. The data collected observations of the size of the weight absolute, survival, FCR, molecular weight DNA composition, heterogeneity genotype, polymorphism. The results showed that highest of polymorphism were T3 (weight absolute 169.18 mg) and lowest ppolymorphism treatment T1 (weight absolute 149.25 mg) .Next analysis of polymorphism results using a micro-satellite in treatment (T2, ribbon 3,4), and (T3, ribbons 5.6) and (T4, ribbons 7 and 8) respectively using IS-GB1F 5-CCC TGT ATT TCA TTT CTC CA-3 and IS-GB 1 R 5-ACC AAC ACT GCA ATC TCT CT-3 bright ribbons found in treatment T4 (ribbons 7.8) snakeheads are the results showed that the growth of snakeheads were highest in T3 (with ribbons 205bp allele ladder with 215 bps and 225 bps on the right, Addition as well as having polymorphism is high and. variation of different types also found the number of alleles and heterozygotes.
蛇头养殖的目标是生产基于不同剂量的富含维生素C的人工饲料的优质产品，并使用微卫星来增加生长和确定优质产品。方法2017年2月至11月，采用完全随机设计（CRD）和4个处理和3个重复，每100g饲料给予0 mg（T1）、12 mg（T2）、24 mg和36 mg（T3）、48 mg（T4）富含维生素C的人工饲料的效果。此外，利用微卫星遗传标记来确定优良品质，以查看蛇头的遗传密码。然后通过方差分析和分子量DNA数据排列对治疗进行分析，通过Cco-stat和Minitab version 11对多态性进行分析。数据收集了观察到的绝对体重大小、存活率、FCR、分子量DNA组成、异质性基因型、多态性。结果显示，多态性最高的是T3（绝对体重169.18mg）和最低的多态性处理T1（绝对体重149.25mg），和（T3，条带5.6）和（T4，条带7和8）分别用IS-GB1F5-CCC TGT ATT TCA TTT CTC CA-3和IS-GB1R5-ACC AAC ACT GCA ATC TCT CT-3处理T4（条带7.8）蛇头的结果表明（带205bp等位基因梯，右边分别为215bps和225bps，加性和多态性较高，不同类型的变异也发现了等位基因和杂合子的数量。
{"title":"Engineering Technology Fish Farming of Snakeheads Channa striat ,Bloch, 1793) Based Feed Vitamin C Increase to Superior Quality Using Microsatellite","authors":"I. Samidjan, D. Rachmawati","doi":"10.20884/1.oa.2018.14.2.571","DOIUrl":"https://doi.org/10.20884/1.oa.2018.14.2.571","url":null,"abstract":"Objectives for snakeheads farming Cngineered to produce superior products based artificial diet eenrichment vitamin C with different doses and the use of microsatellites to increase growth and determine the superior quality. Methods February to November 2017 Examined the Effect of artificial feed Enriched with vitamin C doses per 100 g feed were given 0 mg (T1), 12 mg (T2), 24 mg ,and 36 mg (T3), 48 mg (T4) were using a completely randomized design (CRD) and 4 treatments and 3 replications. Furthermore, to determine the superior quality using microsatellite genetic markers to see the genetic code snakeheads. Then the treatment was analyzed by ANOVA and data arrangement of molecular weight DNA, polymorphism were analyzed by Cco-stat and Minitab version 11. The data collected observations of the size of the weight absolute, survival, FCR, molecular weight DNA composition, heterogeneity genotype, polymorphism. The results showed that highest of polymorphism were T3 (weight absolute 169.18 mg) and lowest ppolymorphism treatment T1 (weight absolute 149.25 mg) .Next analysis of polymorphism results using a micro-satellite in treatment (T2, ribbon 3,4), and (T3, ribbons 5.6) and (T4, ribbons 7 and 8) respectively using IS-GB1F 5-CCC TGT ATT TCA TTT CTC CA-3 and IS-GB 1 R 5-ACC AAC ACT GCA ATC TCT CT-3 bright ribbons found in treatment T4 (ribbons 7.8) snakeheads are the results showed that the growth of snakeheads were highest in T3 (with ribbons 205bp allele ladder with 215 bps and 225 bps on the right, Addition as well as having polymorphism is high and. variation of different types also found the number of alleles and heterozygotes.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43330412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-27DOI: 10.20884/1.OA.2018.14.2.568
D. Puspaningsih, E. Supriyono, K. Nirmala, Iman Rusmana, C. Kusmana, A. Widiyati
Intensive culture of snakehead fish used artificial feed with high protein content. The impact of the high use of artificial feed will cause waste that can cause poor water quality and cause fish mortality. The purpose of this research was to know the dynamics of water quality during culture of snakehead fish Channa striata in the aquarium. The test animals used were snakehead fish with a length of 6.90 ± 0.49 cm and weight of 2.57 ± 0.59 g. Fish was maintenanced with a density of 2 fish L-1, 4 fish L-1 and 6 fish L-1. Fish was cultured for four days in an aquarium with size of 60x40x40 cm3 and water volume of 20 L. Water quality were observed, i.e, temperature, pH, dissolved oxygen, TAN, nitrite, nitrate, orthophosphate, hardness and alkalinity. The results showed that pH, temperature, hardness, TAN, nitrite, nitrate and ortophosphate were not significantly different between treatments (P>0.05), while dissolved oxygen and alkalinity were showed different significantly (P<0.05). On the fourth day of culture with a density of 4 fish L-1 and 6 fish L-1 has led to water saturation in the culture media. Dynamic of water quality was started on the third day of culture, whereas nitrites could not be converted to nitrate maximally. High levels of nitrate in the snakehead culture indicated that that this culture could be combined with an integrated aquaculture with plants.
{"title":"The Dynamics of Water Quality During Culture of Snakehead Fish (Channa striata) in The Aquarium","authors":"D. Puspaningsih, E. Supriyono, K. Nirmala, Iman Rusmana, C. Kusmana, A. Widiyati","doi":"10.20884/1.OA.2018.14.2.568","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.2.568","url":null,"abstract":"Intensive culture of snakehead fish used artificial feed with high protein content. The impact of the high use of artificial feed will cause waste that can cause poor water quality and cause fish mortality. The purpose of this research was to know the dynamics of water quality during culture of snakehead fish Channa striata in the aquarium. The test animals used were snakehead fish with a length of 6.90 ± 0.49 cm and weight of 2.57 ± 0.59 g. Fish was maintenanced with a density of 2 fish L-1, 4 fish L-1 and 6 fish L-1. Fish was cultured for four days in an aquarium with size of 60x40x40 cm3 and water volume of 20 L. Water quality were observed, i.e, temperature, pH, dissolved oxygen, TAN, nitrite, nitrate, orthophosphate, hardness and alkalinity. The results showed that pH, temperature, hardness, TAN, nitrite, nitrate and ortophosphate were not significantly different between treatments (P>0.05), while dissolved oxygen and alkalinity were showed different significantly (P<0.05). On the fourth day of culture with a density of 4 fish L-1 and 6 fish L-1 has led to water saturation in the culture media. Dynamic of water quality was started on the third day of culture, whereas nitrites could not be converted to nitrate maximally. High levels of nitrate in the snakehead culture indicated that that this culture could be combined with an integrated aquaculture with plants.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47261592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-27DOI: 10.20884/1.OA.2018.14.2.569
I. Samidjan, D. Rachmawati
The objective of the study was to assess the role of polyculture engineering of vanamei shrimp, and seaweed in an effort to increase growth and survival. The experimental method was randomized complete design with 4 treatments and 3 replications were T1 (10 PL / m2 white shrimp vannamei and seaweed distance 15 cm), T2 (10 PL / m2 of white shrimp vannamei and seaweed distance 30 cm), T3 (10 PL / m2 white shrimp vanamei and seaweed distance 45 cm, T4 (10 PL / m2 white shrimp vanamei and seaweed 60 cm). Seaweed used Gracillaria sp type 150 g / bundle with treatment used a long line system placed around the nets plot of 1 m2 in pond culture 300 m2. Data includes the growth of absolute weight, survival, FCR were analyzed variety and test Tukey. observation of the water quality of maintenance media include nitrite, ammonia, temperature, pH, salinity using descriptive. There is a very real effect (P <0.01) on treatment with different plant spacing on seaweed and vanamei shrimp on growth and survival of white shrimp vanamei and seaweed. The highest absolute weight growth in vanamei shrimp was T4 treatment (vanamei shrimp 29.25 ± 0.07 g), and seaweed (2919.03 ± 8.70bg) of white shrimp vanamei survival rate (98.25% ± 2.25%) and FCR (food Conversion ratio) 1.15 ± 0.09. Water quality is still feasible optimum.
{"title":"Polyculture Engineering of White Shrimp Vannamei and Seaweed on Different Planting Distance on The Growth, Survival in Abration Pond","authors":"I. Samidjan, D. Rachmawati","doi":"10.20884/1.OA.2018.14.2.569","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.2.569","url":null,"abstract":"The objective of the study was to assess the role of polyculture engineering of vanamei shrimp, and seaweed in an effort to increase growth and survival. The experimental method was randomized complete design with 4 treatments and 3 replications were T1 (10 PL / m2 white shrimp vannamei and seaweed distance 15 cm), T2 (10 PL / m2 of white shrimp vannamei and seaweed distance 30 cm), T3 (10 PL / m2 white shrimp vanamei and seaweed distance 45 cm, T4 (10 PL / m2 white shrimp vanamei and seaweed 60 cm). Seaweed used Gracillaria sp type 150 g / bundle with treatment used a long line system placed around the nets plot of 1 m2 in pond culture 300 m2. Data includes the growth of absolute weight, survival, FCR were analyzed variety and test Tukey. observation of the water quality of maintenance media include nitrite, ammonia, temperature, pH, salinity using descriptive. There is a very real effect (P <0.01) on treatment with different plant spacing on seaweed and vanamei shrimp on growth and survival of white shrimp vanamei and seaweed. The highest absolute weight growth in vanamei shrimp was T4 treatment (vanamei shrimp 29.25 ± 0.07 g), and seaweed (2919.03 ± 8.70bg) of white shrimp vanamei survival rate (98.25% ± 2.25%) and FCR (food Conversion ratio) 1.15 ± 0.09. Water quality is still feasible optimum.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48088391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-21DOI: 10.20884/1.OA.2018.14.1.375
W. A. Tanod, Anita Treisya Aristawati, M. Putra, Muliadin Muliadin
There is a growing need for new antibacterial agents, in particular because many antibiotics are becoming ineffective due to the emergence of resistant bacterial strains. Soft corals of the Genus Sinularia, Family Alcyoniidae, have potential as a source of terpenoid and steroid compounds with antibacterial activity. These corals may vary in external morphology (shape, colour, size).The aim of this research was to identify extracted fractions with high antibacterial activity. Sinularia sp. specimens were extracted, fractionated based on solvent polarity, and tested for antibacterial bioactivity against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Antibacterial activity of the three fractions varied in strength. The dichloromethane fraction showed strong antibacterial activity, inhibiting S. aureus and E. coli growth at a concentration of 1 mg ml-1, while the ethyl acetate and ethanol fractions were effective at 10 mg ml-1 and 100 mg ml-1, respectively.
{"title":"SOFT CORAL (Sinularia sp.) EXTRACTS WITH ANTIBACTERIAL ACTIVITY","authors":"W. A. Tanod, Anita Treisya Aristawati, M. Putra, Muliadin Muliadin","doi":"10.20884/1.OA.2018.14.1.375","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.1.375","url":null,"abstract":"There is a growing need for new antibacterial agents, in particular because many antibiotics are becoming ineffective due to the emergence of resistant bacterial strains. Soft corals of the Genus Sinularia, Family Alcyoniidae, have potential as a source of terpenoid and steroid compounds with antibacterial activity. These corals may vary in external morphology (shape, colour, size).The aim of this research was to identify extracted fractions with high antibacterial activity. Sinularia sp. specimens were extracted, fractionated based on solvent polarity, and tested for antibacterial bioactivity against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Antibacterial activity of the three fractions varied in strength. The dichloromethane fraction showed strong antibacterial activity, inhibiting S. aureus and E. coli growth at a concentration of 1 mg ml-1, while the ethyl acetate and ethanol fractions were effective at 10 mg ml-1 and 100 mg ml-1, respectively.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48781081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-21DOI: 10.20884/1.OA.2018.14.2.566
A. S. Siregar, Sofi Alfiyah, N. A. Prayogo, I. Sulistyo
As a harmful and non-degradable heavy metal, lead (Pb) is also toxic to aquatic organisms. This metal damages tissue and sensitive organs such as gills. A study dealt with the effect of Pb concentration and contact time on gill microanatomy structure of hard-lipped barb fish (Osteochilus hasselti). An experimental method was applied during study with Completely Randomized Design to test 4 concentrations of Pb, i.e 0 mg/L (P0), 8.93 mg/L (P1), 17.86 mg/L (P2) and 26.80 mg/L (P3), in quadruplicates. The gills were removed, and fixed in Bouin solution, from fish sampled twice in a month. The gills were subsequently processed in classical histology to obtain gill microanatomy structure damage data. The data were established from Pantung method, which were then analyzed with One-way ANOVA. The results showed that Pb damaged gills, mildly to severely, in form of oedema, hyperplatia, lamella fusions, and necrosis. Concentration levels and contact time affected microanatomy structure damage of gills. The increasing concentration levels and length of contact time did not gravely increase in damage.
{"title":"Effects of Leads Pb on Gill Microanatomy Structure of Hard-Lipped Barb Fish Osteochilus hasselti","authors":"A. S. Siregar, Sofi Alfiyah, N. A. Prayogo, I. Sulistyo","doi":"10.20884/1.OA.2018.14.2.566","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.2.566","url":null,"abstract":"As a harmful and non-degradable heavy metal, lead (Pb) is also toxic to aquatic organisms. This metal damages tissue and sensitive organs such as gills. A study dealt with the effect of Pb concentration and contact time on gill microanatomy structure of hard-lipped barb fish (Osteochilus hasselti). An experimental method was applied during study with Completely Randomized Design to test 4 concentrations of Pb, i.e 0 mg/L (P0), 8.93 mg/L (P1), 17.86 mg/L (P2) and 26.80 mg/L (P3), in quadruplicates. The gills were removed, and fixed in Bouin solution, from fish sampled twice in a month. The gills were subsequently processed in classical histology to obtain gill microanatomy structure damage data. The data were established from Pantung method, which were then analyzed with One-way ANOVA. The results showed that Pb damaged gills, mildly to severely, in form of oedema, hyperplatia, lamella fusions, and necrosis. Concentration levels and contact time affected microanatomy structure damage of gills. The increasing concentration levels and length of contact time did not gravely increase in damage.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41567609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-14DOI: 10.20884/1.oa.2018.14.2.556
Eni Kusrini, A. Alimuddin, E. P. Hayuningtyas, Syuhada Restu Danupratama
Transfection and electroporation method shave a high possibility to apply towards transgenic production of small eggs size fish species. This study aimed to examine the potential of transfection and electroporation methods to use for transferring a foreign gene into betta fish (Betta splendens) embryos using green fluorescent protein (GFP) gene as a model. Fish were spawned naturally in the ratio of male: female was 1:1, then a total of 200 eggs were taken for each treatment. Transfection was performed for 30 minutes (room temperature of about 25 °C) at two-cell stage of embryos using transfast reagent. Transfection reaction consisted of 0.75 µL transfast reagent, 0.25 µL GFP expression vector (DNA concentration: 50 µg/µL) and 99 µL NaCl solution (concentration: 0,95%). Electroporation was performed using 125 volt cm-1, 3 times pulse frequency at one second interval and pulse length of 7 micro seconds. A volume of 800 µL GFP expression vector solution (DNA concentration: 50 µg/ µL) in PBS was used for electroporation. The successful of foreign gene transfer was determined by PCR method with GFP specific primers. The results showed that hatching rate of eggs in transfection treatment was 67.08%, while the electroporation was 72.09%. Survival of larvae in transfection treatment was 73.00%, while the electroporation was 75.00%. The results of PCR analysis showed that transfection method allowed 65% of the survived fish carrying GFP gene, whereas the electroporation method was 70%. Thus, foreign gene transfer in betta fish can be conducted using the transfection and electroporation methods.
{"title":"Transfer of Green Fluorescent Protein (GFP) Gene to Betta splendens Embryos by Transfection and Electroporation Methods","authors":"Eni Kusrini, A. Alimuddin, E. P. Hayuningtyas, Syuhada Restu Danupratama","doi":"10.20884/1.oa.2018.14.2.556","DOIUrl":"https://doi.org/10.20884/1.oa.2018.14.2.556","url":null,"abstract":"Transfection and electroporation method shave a high possibility to apply towards transgenic production of small eggs size fish species. This study aimed to examine the potential of transfection and electroporation methods to use for transferring a foreign gene into betta fish (Betta splendens) embryos using green fluorescent protein (GFP) gene as a model. Fish were spawned naturally in the ratio of male: female was 1:1, then a total of 200 eggs were taken for each treatment. Transfection was performed for 30 minutes (room temperature of about 25 °C) at two-cell stage of embryos using transfast reagent. Transfection reaction consisted of 0.75 µL transfast reagent, 0.25 µL GFP expression vector (DNA concentration: 50 µg/µL) and 99 µL NaCl solution (concentration: 0,95%). Electroporation was performed using 125 volt cm-1, 3 times pulse frequency at one second interval and pulse length of 7 micro seconds. A volume of 800 µL GFP expression vector solution (DNA concentration: 50 µg/ µL) in PBS was used for electroporation. The successful of foreign gene transfer was determined by PCR method with GFP specific primers. The results showed that hatching rate of eggs in transfection treatment was 67.08%, while the electroporation was 72.09%. Survival of larvae in transfection treatment was 73.00%, while the electroporation was 75.00%. The results of PCR analysis showed that transfection method allowed 65% of the survived fish carrying GFP gene, whereas the electroporation method was 70%. Thus, foreign gene transfer in betta fish can be conducted using the transfection and electroporation methods. ","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41728960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-09DOI: 10.20884/1.OA.2018.14.2.553
S. Sarjito, A. H. C. Haditomo, Desrina Desrina, R. Ariyati, S. B. Prayitno
Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and D14) that were caused 10–55% of fishes get sick and 0–30% fishes mortal. On the other hand, there were 13 isolates do not cause both sick and mortality of fish. On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.
{"title":"The Diversity of Causative Agent Associated With Bacterial Diseases on Catfish (Clarias gariepinus) with Molecular Based from Demak, Indonesia","authors":"S. Sarjito, A. H. C. Haditomo, Desrina Desrina, R. Ariyati, S. B. Prayitno","doi":"10.20884/1.OA.2018.14.2.553","DOIUrl":"https://doi.org/10.20884/1.OA.2018.14.2.553","url":null,"abstract":"Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and D14) that were caused 10–55% of fishes get sick and 0–30% fishes mortal. On the other hand, there were 13 isolates do not cause both sick and mortality of fish. On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.","PeriodicalId":31102,"journal":{"name":"OmniAkuatika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44496533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：