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The Effects of Papain Enzyme Supplement in Feed on Protein Digestibility, Growth and Survival Rate in Sangkuriang Catfish (Clarias sp) 饲料中添加木瓜蛋白酶对桑古良鲶鱼蛋白质消化率、生长和存活率的影响
Pub Date : 2018-08-05 DOI: 10.20884/1.OA.2018.14.2.551
D. Rachmawati, I. Samidjan
The study aimed to assess the effects of Papain Enzyme Supplement in Feed on Protein Digestibity, Growth and Survival Rate of  Sangkuriang Catfish (Clarias sp).  Fingerlings used in the study were acquired from the Center for Freshwater Hatchery and Aquaculture, Muntilan, Central Java, Indonesia. The fingerlings had average weight of 3.43±0.50 g/fish. Completely Randomized Design was used in the experiments with 5 (five) treatment and 3 (three) repetitions. The experimental feed contained 31% of protein and 252.06 Kcal/g of energy. Various doses of the addition of papain enzyme were incorporated into the feed, those doses were A (0 g/kg feed), B (2 g/kg feed), C (4 g/kg feed), D (6 g/kg feed) and E (8 g/kg feed).  Parameters of digestibility of protein (ADCP), relative growth rate (RGR), efficiency of feed utilization (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate (SR) and water quality were evaluated.  The papain enzyme supplement significantly (P<0.01) influenced on ADCp, RGR, EFU, FCR, and PER, otherwise SR of catfish was insignificantly affected by the papain enzyme supplement. The best treatment result was the addition of 6/kg feed papain enzyme (treatment D). The study concluded that the optimum dose of the papain enzyme supplement for ADCp, EFU, FCR, PER and RGR were 5.65, 5.62, 6.0, 5.66, 6.0  g/kg feed respectively. Water quality during study was still in favorable condition for nurturing Sangkuriang catfish (Clarias sp).  
本试验旨在研究饲料中添加木瓜蛋白酶对桑库里良鲶鱼蛋白质消化率、生长和存活率的影响。研究中使用的鱼种取自印度尼西亚中爪哇穆蒂兰淡水孵化场和水产养殖中心。鱼种平均体重为3.43±0.50 g/条。试验采用完全随机设计,5(5)次处理,3(3)次重复。试验饲料中蛋白质含量为31%,能量为252.06 Kcal/g。在饲料中添加不同剂量的木瓜蛋白酶酶,分别为A (0 g/kg饲料)、B (2 g/kg饲料)、C (4 g/kg饲料)、D (6 g/kg饲料)和E (8 g/kg饲料)。评价蛋白质消化率(ADCP)、相对生长率(RGR)、饲料利用效率(EFU)、饲料系数(FCR)、蛋白质效率(PER)、成活率(SR)和水质等参数。木瓜蛋白酶对鲶鱼ADCp、RGR、EFU、FCR和PER的影响极显著(P<0.01),对SR的影响不显著。结果表明,木瓜蛋白酶酶对ADCp、EFU、FCR、PER和RGR的最佳添加量分别为5.65、5.62、6.0、5.66、6.0 g/kg饲料。研究期间水质仍为桑古良鲶鱼(Clarias sp)的良好养殖条件。
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引用次数: 6
Effect of Variant Manure on Generation Time in Daphnia sp. 不同肥料对瑞香产生时间的影响。
Pub Date : 2018-08-03 DOI: 10.20884/1.oa.2018.14.2.550
H. Hamdani, A. Sahidin, T. Hidayat
The research about generation times of Daphnia sp. was carried out on Mei – June 2017 in Biology Laboratory, FPIK UNPAD. The objective of this research was to analyse the generation time of Daphnia sp. in different manure. The research used experimental  method Randomized Completely Block Design (RCBD) with four treatment and three replication. The variant of manure as treatment were chicken manure (A), quail manure (B), cow manure (C), and goat manure (D). The weight for each manure media as many as  2,4 g/l in 1 litre of glass media. The best performance of life table distribution showed from the cow manure, but quail manure treatment give the best of all biological factor, it mean that Daphnias were cultured with quail manure have good performance in biological parameter. 
2017年5月至6月在福建省自然科学院生物实验室进行了水蚤的世代研究。本研究的目的是分析水蚤在不同肥料中的生成时间。本研究采用随机完全块设计(RCBD)的实验方法,四次治疗,三次复制。作为处理的肥料的变体是鸡粪(A)、鹌鹑粪(B)、牛粪(C)和羊粪(D)。每种粪肥培养基的重量在1升玻璃培养基中高达2,4g/l。牛粪对水蚤的生命表分布表现最好,而鹌鹑粪处理则是所有生物因子中表现最好的,这意味着用鹌鹑粪培养水蚤在生物学参数上表现良好。
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引用次数: 0
The Biodiversity of Gastropods In Karapyak Rocky Shores, Pangandaran Region, West Java Province, Indonesia 印度尼西亚西爪哇省潘干达兰地区卡拉皮亚克岩岸腹足类生物多样性
Pub Date : 2018-07-30 DOI: 10.20884/1.oa.2018.14.2.547
A. Sahidin, Z. Zahidah, H. Hamdani, Indah Riyantini, Roni Sewiko
Pangandaran rocky shores have a unique ecosystem and a variant of invertebrate organisms such as mollusk from the gastropod class. This study aims to analyze the biodiversity of gastropods on the rocky shore of Karapyak beach. This research consists of 5 stations. The quadrant set by 1 m x 1 m vertically toward the sea. The result found 773 individuals scattered in nine families. The family of Neritidae dominated (43%), followed by family cyprinoids with 6.3%. Nerita plicata is a species found to dominate in every station with an average abundance (256 ± 10) and/m2. Using the Bray-Curtis cluster analysis, showed variations in the distribution and abundance of different gastropods vertically into the ocean and uniformly distributed horizontally to the shore. Substrate and tidal are the main variables in the spatial distribution of gastropods in the Karapyak beach.
Pangandaran岩石海岸有独特的生态系统和各种无脊椎生物,如腹足类软体动物。本研究旨在分析卡拉皮亚克海滩岩石岸边腹足类动物的生物多样性。本研究由5个站点组成。向海方向1米× 1米的象限。结果发现773只分散在9个家族中。鲤科占多数(43%),鲤科次之(6.3%)。每个站点的平均丰度为(256±10)个/m2。利用布雷-柯蒂斯聚类分析,显示了不同腹足类动物在垂直方向上的分布和丰度的变化,以及在水平方向上向海岸的均匀分布。底物和潮汐是影响卡拉皮亚克滩涂腹足类空间分布的主要因素。
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引用次数: 5
Characteristics of Gel Emulsion Formulation of Snakehead (Channa Micropeltes) As Wound Healer 蛇头伤口愈合剂凝胶乳液配方的研究
Pub Date : 2018-07-25 DOI: 10.20884/1.OA.2018.14.2.545
Firlianty Firlianty, C. Anwar, A. Najamuddin, S. B. Pratasik
This study is aimed to know the characteristics and the effectivity of gel emulsion of snakehead (Channa micropeltes) extract in accelerating wound healing process. It used experimental method with Complete Randomized Design. Four treatments with 3 replications were employed, i.e. 1%, 2%, and 4% gel emulsion of snakehead Channa micropeltes extract and a control treatment. Results showed that C. micropeltes extract formulated in gel emulsion had white colour, typical aroma of gel emulsion, soft texture with mean dispersion ability of 3 cm, and pH 6. In vivo test on male mices that gel emulsion of C. micropeltes extract was highly effective (P≤0.05) to accelerate wound healing, in which the treatment of 1% gel emulsion gave the highest effect with 98.5% wound healing at day-14, followed with that of control treatment, 95% healing, then 2%, 90% healing and 4%, 90% healing, respectively. Snakehead (Channa micrpeltes) extract formulated in gel emulsion can speed up the wound healing process.
本研究旨在了解蛇头提取物凝胶乳的特性及其对伤口愈合的促进作用。采用完全随机设计的实验方法。试验采用1%、2%、4%鱼头微球提取物凝胶乳和对照处理,共4个处理,共3个重复。结果表明,凝胶乳液制得的小檗提取物颜色为白色,具有典型的凝胶乳液香气,质地柔软,平均分散能力为3 cm, pH值为6。在雄性小鼠体内试验表明,微球草提取物凝胶乳对伤口愈合有极好的促进作用(P≤0.05),其中1%凝胶乳处理效果最好,第14天创面愈合98.5%,其次是对照处理,愈合95%,然后分别为2%、90%和4%、90%。以凝胶乳液形式配制的蛇头精华,可加速伤口愈合。
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引用次数: 0
Nutrition of Edible Seaweed Kappaphycus alvarezii Related to Different Environmental Coastal Water Condition 不同海岸水环境条件下食用海藻Kappaphycus alvarezii的营养状况
Pub Date : 2018-07-25 DOI: 10.20884/1.oa.2018.14.2.544
E. N. Dewi, D. Darmanto, A. Ambariyanto
Edible seaweed K. alvarezii collected from different coastal waters in Indonesia (Morowali and Teluk Ekas). The seaweed was extracted into semirefined carrageenan and it was determined on their nutrition quality. The results showed that the essential amino acids content of the three different samples SRC were similar as follows: aspartic acid, glutamic acid, serine, glysine, arginine, alanine, tyrosine, methionine, valine, phenylalanine, isoleucine, leucine and lysine. The gel strength (g/cm2) and viscosities value in Morowali, Ekas coastal waters and commercial SRC were different in the samples. FTIR spectroscopy determined  that  molecular present in three samples were kappa carrageenan.
可食用海藻K. alvarezii收集于印度尼西亚不同的沿海水域(Morowali和Teluk Ekas)。将海藻提取成半精制的卡拉胶,并对其营养品质进行了测定。结果表明,3种不同SRC样品的必需氨基酸含量相似:天冬氨酸、谷氨酸、丝氨酸、甘氨酸、精氨酸、丙氨酸、酪氨酸、蛋氨酸、缬氨酸、苯丙氨酸、异亮氨酸、亮氨酸和赖氨酸。样品中Morowali、Ekas沿海水域和商用SRC的凝胶强度(g/cm2)和粘度值不同。傅里叶红外光谱测定了三个样品中存在的分子为卡帕卡拉胶。
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引用次数: 3
Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture 17α-甲基睾酮对不同盐度培养下热带鳗鲡、双色鳗鲡雄性化的影响
Pub Date : 2018-07-25 DOI: 10.20884/1.oa.2018.14.2.546
F. Rachmawati, R. Affandi, Y. Sistina
Eel population in nature reaches critical number, so that culture strategy is urgently needed to fulfil the high demand of this fish. A shortcut to get functional male, which proven difficult to be founded from natural catching, is masculinization. This research aimed to induce masculinization of tropical eel, Anguilla bicolor McClelland supplemented with various doses of 17α-methyltestosterone during a month culture in freshwater (0 ppt) or brackish water (10 ppt).  The eel was grouped and fed with supplementation of 17α-methyltestosterone at various doses, depending upon treatments, namely 0 mg Kg-1 diet (control), 40, 80 or 120 mg Kg-1 diet. Eels size were similar, at approximately 16,78 g ± 0,62 in weight and 25,38 cm ± 0,15 in length were either culture in freshwater or brackish during the experiment for eight weeks. Sex gonad, based on anatomical histological structures, Eye Index and Fin Index were measured after time culture treatment achieved, as well as body length, weight, eye diameter and the length of the pectoral fin were measured. Results showed that supplementation 17α-methyltestosterone 80 mg/Kg diet culture in brackish water has the highest number of male (90%).  This study proven that, the hormone was effective for masculinization in eels, It useful for masculinization in eels. Results proved that the 17α-methyltestosterone highly significant (P<0.01) effect on the Eye index (3.63 – 5.14) and Fin Index (3.03 – 4.08) of eels. This study concluded, that 17α-methyltestosterone more effective in improving the number of males in brackish water than in freshwater culture.
鳗鲡在自然界的种群数量已达到临界数量,迫切需要制定相应的养殖策略来满足对鳗鲡的高需求。获得功能性雄性的捷径是男性化,这很难从自然捕获中建立起来。本研究在淡水(0 ppt)或半咸淡水(10 ppt)中添加不同剂量的17α-甲基睾酮,诱导热带鳗鲡(Anguilla bicolor McClelland)雄性化。将鳗鲡分组,根据处理情况,分别添加不同剂量的17α-甲基睾酮,即0 mg Kg-1日粮(对照)、40、80或120 mg Kg-1日粮。鳗鱼的大小相似,在淡水或半咸淡水中培养8周,体重约为16.78 g±0.62,长度约为25.38 cm±0.15。根据性腺解剖组织学结构,测定时间培养完成后的眼指数和鳍指数,测定体长、体重、眼直径和胸鳍长度。结果表明,在微淡水中添加17α-甲基睾酮80 mg/Kg的日粮培养物,雄鱼数量最高(90%)。本研究证明,该激素对鳗鱼的雄性化是有效的,对鳗鱼的雄性化是有益的。结果表明,17α-甲基睾酮对鳗鱼眼指数(3.63 ~ 5.14)和鳍指数(3.03 ~ 4.08)的影响极显著(P<0.01)。本研究表明,在咸淡水中,17α-甲基睾酮对提高雄鱼数量的效果优于淡水养殖。
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引用次数: 1
Micro/Nano Bubble Technology : Characteristics and Implications Biology Performance of Koi Cyprinus carpio in Recirculation Aquaculture System (RAS) 微/纳米气泡技术:循环水养殖系统中鲤的特性及生物学性能
Pub Date : 2018-07-23 DOI: 10.20884/1.OA.2018.14.2.539
H. K. Saputra, K. Nirmala, E. Supriyono, N. Rochman
Technology for aquaculture is growing with the high demand of fish. Micro / nano bubble is one of technology that is developed to increase dissolved oxygen in water. This technology is used to increase Koi fish production in high density via RAS. The purpose of this study was to determine the characteristics of micro / nano bubble technology and its effect on biology of koi fish (Cyprinus carpio). Lutor was used to introduce micro / nano bubble in the fish tank by using fish length (e.g. 7.49 ± 0.29 cm). This research used completely randomized design with one factor (fish density) and three replications. This factor was three densities of fish at 15 fish.60 L-1 (A), 30 fish.60 L -1 (B), and 45 fish. 60 L -1 (C). The result showed that there was the absolute length of each treatments was (0.52 ± 0.03a) cm for A, treatment  B (0.36 ± 0.07ab) cm and C treatment (0.29 ± 0.08b) cm. The best treatment is A treatment (15 fish. 60 L -1) and different significant in statistic with C treatment (45 fish. 60 L-1) but not significant in statistic with B treatment (30 fish.­ 60 L -1).
随着对鱼类的高需求,水产养殖技术也在不断发展。微/纳米气泡是为增加水中溶解氧而开发的技术之一。这项技术用于通过RAS提高高密度锦鲤的产量。本研究的目的是确定微/纳米气泡技术的特点及其对鲤鱼生物学的影响。Lutor用于通过使用鱼的长度(例如7.49±0.29 cm)在鱼缸中引入微/纳米气泡。本研究采用一因素(鱼类密度)和三次重复的完全随机设计。该因子是三种密度的鱼,分别为15条鱼.60 L-1(A)、30条鱼.60 L-1(B)和45条鱼。60升-1(C)。结果表明,各处理的绝对长度分别为(0.52±0.03a)cm、(0.36±0.07ab)cm和(0.29±0.08b。
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引用次数: 7
Phytoremediation of Eel (Anguilla bicolor bicolor) rearing wastewater using amazon sword (Echinodorus amazonicus ) and water jasmine (Echinodorus palaefolius) 亚马逊剑(Echinodorus amazonicus)和茉莉花(Echindorus palaefolius)对鳗鱼养殖废水的植物修复
Pub Date : 2018-07-23 DOI: 10.20884/1.OA.2018.14.2.541
H. Handajani, W. Widanarni, T. Budiardi, M. Setiawati, S. Sujono
Management of aquaculture wastewater is still the major problems in fisheries, especially in intensive systems. Intensively aquaculture activities often increase organic matter and nutrients (N and P) in the water.  The study was conducted to evaluate the phytoremediator performance of Echinodorus amazonicus and Echinodorus palaefolius in removing inorganic nitrogen and orthophosphate from aquaculture wastewater with the recircula­tion system. This study used a completely randomized design with three treatments and 3 replications. The treatments of this study were P1: E. amazonicus,  P2: E. palaefolius and P3: control (without aquatic plants). The tested fish were elvers  which average weight 6.98 ± 0.19 g,  and  fish density was 4 gL-1. Elvers reared in an aquarium that containing 48 L in  a recirculation system. The paste feed which protein level of  45.25% was given at satiation 3 times/day. Elvers reared for 60 days. The results showed that E. palaefolius significantly reduced concentrations of total ammonia nitrogen (TAN), nitrite (NO2), nitrate (NO3) and orthophosphate (PO4) with an efficiency of 27.10 ± 2.42%; 45.03 ± 9.77%; 20.94 ± 1.29% and 14.19 ± 3.05%, respectively higher than E. amazonicus and control. The best result of elver’s performance (SGR and FCR) was in treatment P2 (E. palaefolius), i.e.  1.19 ± 0.18% and 1.57 ± 0.30. Based on the results of this study, it can be concluded that the use of E. palaefolius as phytoremediator in eel culture with recirculation system can removed inorganic nitrogen and orthophosphate more optimal compared to other treatment. 
水产养殖废水的管理仍然是渔业的主要问题,特别是在集约化系统中。集约化的水产养殖活动往往会增加水中的有机质和营养物(氮和磷)。研究了亚马逊棘蚓和古叶棘蚓在循环水系统中去除水产养殖废水中无机氮和正磷酸盐的修复效果。本研究采用完全随机设计,3个处理,3个重复。本研究处理为P1: amazonicus、P2: palaefolius和P3:对照(无水生植物)。试验鱼为平均体重为6.98±0.19 g的elver,鱼密度为4 gL-1。在循环系统中含有48升的水族箱中饲养的小鳗。饲喂蛋白质水平为45.25%的膏状饲料3次/d。饲养60天。结果表明:古叶杆菌显著降低了总氨氮(TAN)、亚硝酸盐(NO2)、硝酸盐(NO3)和正磷酸盐(PO4)的浓度,效率为27.10±2.42%;45.03±9.77%;与对照相比,分别高出20.94±1.29%和14.19±3.05%。elver的SGR和FCR以P2处理为最佳,分别为1.19±0.18%和1.57±0.30。综上所述,在循环式鳗鲡养殖中,古叶芽孢杆菌作为植物修复剂对无机氮和正磷酸盐的去除效果优于其他处理。
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引用次数: 9
The Osmotic Response and Hydromineral Status of Transported Anguilla bicolor bicolor Glass Eels with Various Ratios of Biomass and Water Volume 不同生物量和水量比下运输双色鳗和双色玻璃鳗的渗透响应和水矿物状态
Pub Date : 2018-07-23 DOI: 10.20884/1.OA.2018.14.2.536
F. H. Taqwa, E. Supriyono, T. Budiardi, M. Setiawati, B. Utomo, R. Affandi
This research was conducted to determine the closed transportation system with biomass and water volume ratio that can support the osmoregulation ability of glass eel significantly. Glass eel was obtained from catching activities in the Cimandiri River, Pelabuhan Ratu (mean length 52.40+0.84 mm and weight 0.10+0.01 g) and acclimatized for 24 hours at salinity 6 mg L-1 and temperature 20°C gradually.The research design was a completely randomized design. The applied treatment was glass eel closed transportation system with various ratio of glass eel biomass (kg): water volume (L), i.e. 1:11; 1:12; 1:13; and 1:14 during 24 hours of land transportation and continued with a 3-day recovery period.The water was used refer to the above acclimatization method, with ratio the water part of pure oxygen in a plastic bag was 1:3. The measured parameters include survival rate, osmotic gradient, body hydromineral, and water quality in transport packaging and recovery medium. Survival rates were generated in the high range until recovery period (P<0.05), but the transportation method caused differences significantly (P<0.05) in osmotic response and hydromineral status (sodium, chloride, potassium, and water content), so that it will affect production performance in the next stage of culture. Ratio of glass eel biomass: water volume of 1:13 has been able to support the ability of osmoregulation glass eel to maintain homeostatic condition during the transportation until 3 days of recovery period. There was no drastic decline in physical and chemical value of water in the transport bag so it could reduce the mortality rate for 24 hours.
本研究旨在确定具有生物量和水体积比的封闭运输系统,该系统能够显著支持玻璃鳗鱼的渗透调节能力。玻璃鳗是在Pelabuhan Ratu的Cimandiri河(平均长度52.40±0.84 mm,重量0.10±0.01 g)进行捕捞活动获得的,并在盐度6 mg L-1和温度20°C下逐渐驯化24小时。研究设计是完全随机的。应用的处理是玻璃鳗鱼封闭运输系统,玻璃鳗鱼生物量(kg)与水量(L)的比例不同,即1:11;1:12;1:13;在24小时的陆路运输中为1:14,并持续了3天的恢复期。参照上述驯化方法使用水,塑料袋中纯氧的水比例为1:3。测量的参数包括存活率、渗透梯度、身体水矿物质以及运输包装和回收介质中的水质。在恢复期之前,存活率都在较高范围内(P<0.05),但运输方法在渗透反应和水矿物质状态(钠、氯、钾和水含量)方面造成了显著差异(P<0.05)。因此,运输方法将影响下一阶段培养的生产性能。玻璃鳗鱼生物量的比例:1:13的水量能够支持玻璃鳗鱼在运输过程中保持稳态的渗透调节能力,直到3天的恢复期。运输袋中的水的物理和化学价值没有急剧下降,因此可以在24小时内降低死亡率。
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引用次数: 1
Bioremediation of Indigosol Blue 04B Batik Effluent by Indigenous Fungal Isolates, Aspergillus spp. 本土真菌曲霉对靛蓝04B蜡染废水的生物修复
Pub Date : 2018-07-23 DOI: 10.20884/1.OA.2018.14.2.537
R. S. Dewi, R. Kasiamdari, E. Martani, Y. A. Purwestri
Effluent from the local batik home industry is a serious problem, because the effluent discharge generated is spread in different places. Untreated effluent can cause environmental pollution, such as in groundwater reservoirs,because most is discharged into rivers. The aim of this research was to evaluate the bioremediation potential of indigenous fungi in liquid culture media with Indigosol Blue 04B (IB) batik effluent. The fungi isolates tested were Aspergillus sp. 1, Aspergillus sp. 2 and Aspergillus sp. 3, isolated from dye effluent soil and batik effluent, and compared to white rot fungi (Phanerochaete chrysosporium) as a positive control.   The physiochemical properties of IB batik effluent before and after fungal treatment were investigated. All of these parameters before the fungal treatment were above the recommended standard values based on the Governor regulation of Yogyakarta Special Region No. 7/2010. The level of biochemical oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), and electrical conductance (EC) was reduce by Aspergillus spp. The highest percentage reduction was achieved by Aspergillus sp. 3, namely 88.34% BOD, 89.11% COD, 75.77% TSS, 85.85% TDS and 71.21% EC, after 3 days of incubation. These results show that the positive control isolate had the lowest value. The study confirms the ability of indigenous fungi isolates in the remediation of IB batik effluent and their potential for future analysis in the treatment of all types of batik effluent.
当地蜡染家居工业的污水排放是一个严重的问题,因为所产生的污水排放分布在不同的地方。未经处理的污水会造成环境污染,例如地下水水库,因为大多数污水被排入河流。本研究的目的是评价靛蓝04B (IB)蜡染废水在液体培养基中对土生真菌的生物修复潜力。从染料废水土壤和蜡染废水中分离的真菌为曲霉1、曲霉2和曲霉3,并与阳性对照白腐菌(Phanerochaete chrysosporium)进行比较。对真菌处理前后IB蜡染废水的理化性质进行了研究。真菌处理前的所有这些参数均高于日惹特区第7/2010号总督条例所建议的标准值。结果表明,曲霉可降低生物生化需氧量(BOD)、化学需氧量(COD)、总溶解固形物(TDS)、总悬浮固形物(TSS)和电导率(EC),其中以曲霉3的降低率最高,分别为88.34%、89.11%、75.77%、85.85%和71.21%。结果表明,阳性对照分离物的该值最低。该研究证实了本地真菌分离物在IB蜡染废水修复中的能力,以及它们在未来分析处理所有类型蜡染废水的潜力。
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引用次数: 11
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OmniAkuatika
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