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Daya Saing Kawin Nyamuk Jantan Steril (Culex quinquefasciatus) Skala Laboratorium: Studi Awal Penggunaan Teknik Serangga Mandul dalam Pengendalian Vektor Filariasis Limfatik di Kota Pekalongan 无菌雄蚊子的交配能力(Culex quinquefasciatus)在实验室规模:在庇加兰市对不育昆虫传播媒介控制的研究
Pub Date : 2017-06-30 DOI: 10.22435/BLB.V13I1.6024.65-72
Tri Ramadhani, Upik Kusuma Hadi, Susi Soviana, Z. Irawati, S. Sunaryo
Culex quinquefasciatus is the main vector of limfatic filariasis in Pekalongan City. Sterile Insect Tehnique could be an alternative vector control efforts to eliminate filariasis. The success of this technique is depend on the ability of laboratory-reared sterile males with the wild-type females. Indicator of SIT Aplication is determined by the value of the mating competitiveness and sterility to Culex quinquefasciatus (Diptera:Culicidae). The design of the research is an experimental. Gamma irradiation on the pupae (age . 15 hours) with the  doses of 0 Gy, 60 Gy, 65 Gy,70 Gy, 75 Gy and 80 Gy in BATAN Jakarta.  Male mosquitoes which emerged from the pupa then matting with a normal female. This research observed the mean  of females laying eggs ,fecundity, fertility and  mating competitiveness. This experimental research was conducted in the laboratory and the data were analyzed by ANOVA.The result showed that irradiation at the trial doses had an effect on fertility of Culex quinquefasciatus, but not  had significant effect on  fecundity and mating competitiveness . A dose of 70 Gy is the optimum dose with a fertility rate of 1.8% (sterility 98.2%) and C indexs 0,568 can be recommended for futher  semi field assays. The number of sterile males were six times compared with the wild population to increase the chances of  mating with wild-type females.
致倦库蚊是贝卡隆安市主要的丝虫病媒介。无菌虫Tehnique可能是消灭丝虫病的另一种媒介控制措施。这项技术的成功取决于实验室饲养的不育雄性和野生型雌性的能力。SIT应用的指标是由对致倦库蚊的交配竞争力和不育性的值决定的。研究的设计是一个实验。在雅加达巴丹对15小时龄的蛹进行0 Gy、60 Gy、65 Gy、70 Gy、75 Gy和80 Gy剂量的γ射线照射。从蛹中出来的雄蚊与一只正常的雌蚊交配。本研究观察了雌性的平均产卵量、繁殖力、生育能力和交配竞争力。本实验在实验室进行,并对数据进行方差分析。结果表明,试验剂量的辐照对致倦库蚊的生育能力有影响,但对其繁殖力和交配竞争力没有显著影响。70 Gy的剂量是最佳剂量,生育率为1.8%(不育率为98.2%),C指数为0568可用于进一步的半田间测定。不育雄性的数量是野生种群的六倍,以增加与野生型雌性交配的机会。
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引用次数: 1
Preferensi Aedes aegypti Meletakkan Telur pada Berbagai Warna Ovitrap di Laboratorium 埃及伊蚊偏好在实验室放置各种颜色的卵
Pub Date : 2017-06-30 DOI: 10.22435/BLB.V13I1.4825.37-42
Made Agus Nurjana, Ade Kurniawan
Dengue Haemograffic fever is a vector borne disease which caused outbreaks and death. There is no applied vaccine until now, so the effort of prevention and control is to terminate chain of infection mosquito breeding. Factors which influenced  the female mosquitoe to lay their eggsare type of container, color, water, temperature, water source, humidity and environment condition. This study was conducted to determine the preferences of Ae. aegypti mosquitoes to lay aggs in various colors ovitrap in the laboratory of Balai Litbang P2B2 Donggala, January until March 2015. Three repetitions with plastic cup black, blue, white, yellow and pink have been performed with water and filter pappers. 30 mosquitoes blood saturation included in the containers with various colors. The result showed that most of female mosquito laid their eggs in plastic cup black (53,2%). ANOVA analysis showed that the diversity of colors ovitrap produce different the number of eggs Ae. aegypti in each type of countainer color. It is recommended to use black ovitrap for controling populations of Ae, aegypti in environment with regular monitoring.
登革出血热是一种媒介传播疾病,可引起疫情暴发和死亡。到目前为止,还没有应用的疫苗,因此预防和控制的努力是终止感染链,蚊子滋生。影响雌蚊产卵的因素有容器类型、颜色、水、温度、水源、湿度和环境条件。本研究是为了确定伊蚊的偏好。2015年1月至3月,在东加拉Balai Litbang P2B2实验室,研究了埃及伊蚊在不同颜色的诱卵器中产卵的情况。用塑料杯(黑色、蓝色、白色、黄色和粉色)用水和滤纸重复了三次。30只蚊子血饱和度不同颜色的容器。结果表明,雌蚊产卵以杯黑色居多(53.2%);方差分析表明,诱卵器颜色的多样性产生的卵数不同。埃及伊蚊在每种容器中都有颜色。建议使用诱蚊黑诱卵器控制环境中埃及伊蚊的数量,并定期监测。
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引用次数: 8
Gambaran Angka Prevalensi Mikrofilaria di Kabupaten Banyuasin Pasca Pengobatan Massal Tahap III III期大规模治疗基线胶囊中血友病患病率的图像
Pub Date : 2017-06-30 DOI: 10.22435/BLB.V13I1.4794.11-20
R. Oktarina, S. Santoso, Y. Taviv
Filariasis is a chronic infectious disease that can cause permanent disability. One effort to  interrupt transmission of filariasis is the Mass Drug Administration filariasis (MDA filariasis) in endemic areas of filariasis for 5 years. In 2012, MDA filariasis was carried out in all regions of Banyuasin. After treatment the 3rd year, it is necessary to evaluate the prevalence of microfilaria and microfilaria density. This activity  was conducted by finger blood survey to people who’s living in sentinel and spot villages. This study selected  930 people  for SDJ activities The test results obtained  4 positive of microfilaria Brugia malayi. The prevalence or microfilaria rate (Mf rate) in the village of sentinel was 0,96 with microfilaria density were 938/mL of blood, Mf rate in spot village was 0.31 with a microfilaria density were 217.75/mL of blood, while the Mf rate district was 0.43 with a microfilaria density were 716.06/mL of blood. People who suffer filiariasis mostly are  farmers/fishermen. The prevalence of microfilariae in Banyuasin decreased, but the risk of transmission can still occur because of the density of microfilariae found> 50/mL of blood.
丝虫病是一种慢性传染病,可导致永久性残疾。阻断丝虫病传播的一项努力是在丝虫病流行地区实施为期5年的大规模药物管理局丝虫病(MDA丝虫病)。2012年,Banyuasin的所有地区都发生了MDA丝虫病。治疗第3年后,有必要评估微丝蚴的患病率和微丝蚴密度。这项活动是通过对居住在哨点和定点村庄的人进行手指血液调查来进行的。本研究选取930人进行SDJ活动,检测结果为4例马来丝虫微丝蚴阳性。哨点村的微丝蚴率(Mf率)为0.96,微丝蚴密度为938/mL血液,点村的Mf率为0.31,微丝酵母菌密度为217.75/mL血液,而Mf率区为0.43,微丝线虫密度为716.06/mL血液。患丝虫病的人大多是农民/渔民。Banyuasin微丝蚴的患病率降低了,但由于血液中微丝蚴密度大于50/mL,传播风险仍然存在。
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引用次数: 5
Pengobatan Malaria Kombinasi Artemisinin (ACT) di Provinsi Papua Barat Tahun 2013 2013年西巴布亚省青蒿素联合治疗疟疾
Pub Date : 2017-06-30 DOI: 10.22435/blb.v13i1.4921.43-54
Revi Rosavika Kinansi, R. Mayasari, Diana Andriyani Pratamawati
Malaria is still a disease with highest incidence rate in Indonesias. Based on Riskesdas 2013, the prevalence of malaria in West Papua was generally increasing. This study aimed to find the correlation of  type of malaria found in blood examination and distribution status of ACT.  Samples of data obtained by stratified random sampling from 1490 people who had suffered from malaria in West Papua. Data analysis using univariate descriptive and correlation analysis The result showed that the most common type of malaria was tertiana malaria caused by Plasmodium vivax (51%). Early detection performed within the first 24 hours when the patient is suffering from fever can be used as the basis for a policy that early detection can reduce malaria morbidity. It can be concluded ACT suitable for any type of malaria. The concistency of provision of ACT can be implemented by increasing public awareness of taking prophylactic. In addition the ideal combination antimalarial drugs be  able to heal in a short time and if the patients performs the compliance of taking the drug, it will not be antimalarial resistance.
疟疾仍然是印尼发病率最高的疾病。根据2013年Riskesdas的数据,西巴布亚的疟疾流行率总体呈上升趋势。本研究旨在探讨血液检查中发现的疟疾类型与ACT分布状况的相关性。通过分层随机抽样从西巴布亚1490名疟疾患者中获得的数据样本。数据分析采用单变量描述性和相关性分析结果表明,最常见的疟疾类型是间日疟原虫引起的间日疟(51%)。在患者发烧的前24小时内进行的早期检测可以作为早期检测可以降低疟疾发病率的政策的基础。可以得出ACT适用于任何类型的疟疾。ACT规定的一致性可以通过提高公众对预防措施的认识来实施。此外,理想的抗疟药物组合能够在短时间内治愈,如果患者遵守服药规定,就不会产生抗疟耐药性。
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引用次数: 4
Perbandingan Indeks Larva Vektor Demam Berdarah Dengue Pra dan Paska-Intervensi di Kota Prabumulih 登革热出血热前和入侵后对该镇的幼虫数量比较
Pub Date : 2017-06-30 DOI: 10.22435/blb.v13i1.5324.55-64
Hotnida Sitorus, Y. Taviv, Anif Budiyanto, L. Ambarita, Milana Salim, R. Mayasari
Prabumulih city is one of dengue endemic area in South Sumatera Province with number of cases in 2011-2012 were 225 and 301 cases respectively. The research aims to determine the influence of jumantik accompanied by health promotion program to the larval mosquitoes indices. This is quasi-experimental study, carried out in three endemic areas of Prabumulih City in 2014. There were two intervention in two location and one location for non-intervention. In the first location, the intervention were larva surveillance by jumantik cadre, giving larvicide selectively, accompanied by health promotion program to community group (intervention I), in second location the intervention only for larvae surveillance by jumantik cadre (intervention II), and the third location giving no intervention.      Observation of immature mosquito was carried out in every location before and after intervention was given. The result showed an increase in Free larvae index of 19,8% in the intervention I , 12,5% in the intervention II and 5,3% in the no-intervention location. Indicator of breteau index showed a decrease in all location, 73,1% in the intervention I, 62,8% in the intervention II and 10,8% in the no-intervention location. Container index indicator showed a decrease in two intervention location, 10,2% in the intervention I, 6,6% in the intervention II, while in no-intervention location show an increase for 8%. Health promotion program about how to prevent dengue transmission through potential community groups could become an alternative effort to control vector population integrated with other control methods.
普拉武里市是南苏门答腊省登革热流行区之一,2011-2012年病例数分别为225例和301例。本研究旨在确定健康促进方案对蚊幼虫指数的影响。这是一项准实验研究,于2014年在Prabumulih市的三个流行区开展。两个位置有两个干预,一个位置没有干预。在第一个地点,干预措施为巨虫干部监测幼虫,选择性给予杀幼虫剂,并辅以社区健康促进计划(干预措施一);在第二个地点,干预措施仅为巨虫干部监测幼虫(干预措施二),第三个地点不进行干预。干预前后各地点均进行幼蚊观察。结果表明,干预1、干预2和无干预3个地点的游离幼虫指数分别增加19.8%、12.5%和5.3%。布雷图指数各测点均有所下降,干预1测点下降73.3%,干预2测点下降62.8%,无干预测点下降10.8%。容器指数指标在两个干预点均有所下降,干预点为10.2%,干预点为6.6%,而无干预点则上升了8%。关于如何通过潜在社区群体预防登革热传播的健康促进规划可与其他控制方法相结合,成为控制病媒种群的另一种努力。
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引用次数: 2
Kriptosporidiosis di Indonesia 印度尼西亚Kriptosporidiosis
Pub Date : 2017-06-30 DOI: 10.22435/BLB.V13I1.4540.73-82
Tri Wijayanti
Cryptosporidiosis included to waterborne and soil transmited diseases, caused by Cryptosporidium, obligat intraceluller pathogen organism. Cryptosporidium cause intestinal infection of human and animal acute diarrhea. Lung cryptosporidiosis on HIV/AIDS and tuberculosis  patients was reported. Diarrhea still be important health problem because diarrhea was be the third dominant contributor of children morbidity and mortality at some country include Indonesia. Every children have 1,6-2x diarrhea onset annually. Diarrhea cases caused by Cryptosporidium sp parasite was around 4-11%. Focus of this review is  about cryptosporidiosis on children, HIV/AIDS and tuberculosis patients, animal, environment, diagnostic and it’s prevention and control. Cryptosporidium species confirmed in Indonesia are C. wrairi, C. muris,  C. felis, C. hominis, C. meleagridis and C. parvum, indicated that there was a big rule of animal on Cryptosporidium transmission. Cryptosporidium was necessary to be one of diseases diagnose on HIV/AIDS and tuberculosis patients. Transmission of Cryptosporidium by  respiratory secretion (droplet, aerosol or contact with vomiting) must be anticipated to prevent cryptosporidiosis especially on imunocompromissed/imunodeficiency people. Rapid Diagnostic Test that have highly sensitivity and spesificity is very important on Cryptosporidium cases finding and surveillance in Indonesia. Environment and cattle sanitation, personal hygiene, water and food treatment, is necessary to prevent cryptosporidiosis transmission.
隐孢子虫病包括由隐孢子虫引起的水传播和土壤传播疾病,隐孢子虫是专性淋室内病原体。隐孢子虫引起人和动物急性腹泻的肠道感染。报道了艾滋病病毒/艾滋病和肺结核患者的肺部隐孢子虫病。腹泻仍然是一个重要的健康问题,因为在包括印度尼西亚在内的一些国家,腹泻是导致儿童发病率和死亡率的第三大因素。每个儿童每年都有1.6-2倍的腹泻发作。由隐孢子虫引起的腹泻病例约占4-11%。本文就隐孢子虫病在儿童、HIV/AIDS和结核病患者、动物、环境、诊断及其预防和控制方面的研究进展进行综述。在印度尼西亚确认的隐孢子虫种类有C.wrairi、C.muris、C.felis、C.hominis、C.meleagridis和C.parvum,这表明隐孢子虫的传播有很大的动物规律。隐孢子虫是诊断艾滋病和肺结核的必要疾病之一。必须预计隐孢子虫通过呼吸道分泌物(飞沫、气溶胶或与呕吐物接触)传播,以预防隐孢子虫病,尤其是在免疫功能低下/免疫功能不足的人群中。快速诊断试验具有高度的敏感性和特异性,对印尼隐孢子虫病例的发现和监测具有重要意义。环境和牲畜卫生、个人卫生、水和食物处理,是预防隐孢子虫病传播的必要条件。
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引用次数: 1
Faktor Lingkungan dan Perilaku yang Berhubungan dengan Kejadian Malaria di Kecamatan Panyabungan Mandailing Natal Sumatera Utara 苏门答腊岛北部与疟疾有关的环境和行为因素纳塔尔-曼德勒关系
Pub Date : 2017-06-30 DOI: 10.22435/blb.v13i1.4672.1-10
Ahmad Faizal Rangkuti, S. Sulistyani, Nur Endah W
Panyabungan is one of the districts in Mandailing Natal regency which is an endemic area of malaria. The number of Malaria cases until 2013 reached 36.6 ‰ in 2879 positive cases. This study aimed to determine factors related to the malaria’s occurrence. This research was observational analytic study with case control design, analyzed by logistic regression.. The result of study indicated that the area of study was in the plateu, the average of temperature and humidity is 30.8 ° C and 66.7% during the day but the everage at night is on 27.2 ° C and 71.7. Factors which  significantly associated with the occurence of malaria were the use of mosquito nets (p value: 0.000; OR: 3.573 ; 95% CI: 1.732 to 7.373), the use of anti-mosquito  substance  (p value: 0.029; OR: 2.719; 95% CI: 1.087 to 6.798), had activity outside  of the house at night (p value: 0.01; OR: 3.254; 95% CI: 1.563 to 6.777), the use of long clothes (p value: 0.013; OR: 2.474; 95% CI: 1.205 to 5.076) and the presence of stagnant water (p value: 0.033; OR: 2.33; 95% CI: 1.06 to 5.118). The dominant risk factors was not using mosquito nets at night.  
Panyabungan是Mandailing Natal县的一个地区,该县是疟疾流行区。截至2013年,在2879例阳性病例中,疟疾病例数达到36.6‰。本研究旨在确定与疟疾发生相关的因素。本研究采用病例对照设计,采用logistic回归分析。。研究结果表明,研究区域位于高原,白天的平均温度和湿度分别为30.8°C和66.7%,而夜间的平均温度为27.2°C和71.7%。与疟疾发生显著相关的因素是蚊帐的使用(p值:0.000;OR:3.573;95%CI:1.732至7.373)、防蚊物质的使用(p值:0.029;OR:2.719;95%CI:1.087至6.798)、夜间有户外活动(p值0.01;OR:3.254;95%CI:1.563至6.777),主要危险因素为夜间不使用蚊帐。
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引用次数: 9
Parameter Entomologi pada Daerah Endemis Demam Berdarah Dengue Tinggi dan Rendah di Jawa Tengah (Studi di Kabupaten Kudus dan Wonosobo) 恩德米斯需求血登革热高、中低区的昆虫学参数(Holy Cape和Wonosobo研究)
Pub Date : 2017-06-30 DOI: 10.22435/BLB.V13I1.4818.29-36
Bina Ikawati, Bondan Fajar Wahyudi, N. Astuti, S. Sunaryo
Kudus district is a district with high DHF cases in  Central Java in 2013 (IR=57.50). Wonosobo District is new area with low DHF cases (IR=11.92). Aedes aegypti is the main vector of DHF in both. The purpose of this study was to determine the difference of entomological parameter in high and low DHF endemic area. This research was continued analysis of entomologycal data from study of The Resistance Map of Aedes aegypti (Linn)  Agains Cypermethrin 0,05%, Malathion 0,8% and Temephos inn Purworejo, Kebumen, Pekalongan, Demak, Wonosobo, Cilacap, Kudus, Klaten, Banjarnegara  at 2014, that use cross sectional design. The results showed that HI, CI, BI and  PI  in Kudus (40,67%,21,40%, 233,67 and 113,67%), higher than in Wonosobo (14,33%, 10,93%, 15,33 dan 38,33%). In all location bathing tub were the potential breeding places of Ae. aegypti. In Kudus, Breeding Preference Ratio (BPR) in barrel and water tank for “wudhu” had the highest as much as 4.03. In Wonosobo the highest BPR was in jar as much as 9.15. Multivariat analysis showed that in Kudus container covers were most closely correlated to the presence of  Ae. aegypti larvae, while in Wonosobo illumination around containers is the main factor.
库杜斯县是中爪哇2013年登革出血热高发地区(IR=57.50)。沃诺索博区为新发低病例区(IR=11.92)。埃及伊蚊是这两个国家登革出血热的主要媒介。本研究的目的是确定登革出血热高、低流行区昆虫学参数的差异。本研究对2014年埃及伊蚊对普瓦雷霍、克布门、佩卡隆干、德马克、沃诺索博、奇拉卡普、库德斯、克laten、班贾尼加拉等地氯氰菊酯0.05%、马拉硫磷0.8%和双硫磷抗性图的昆虫学数据进行了继续分析,采用横断面设计。结果表明,库杜斯的HI、CI、BI和PI分别为40、67%、21、40%、233、67和113、67%,均高于沃努索布(14、33%、10、93%、15、33和38、33%)。浴盆是伊蚊孳生的潜在场所。蚊。在库库中,桶和水箱中“五胡”的育种偏好比(BPR)最高,达4.03。在Wonosobo,最高的BPR是jar,高达9.15。多变量分析表明,库杜斯容器盖与伊蚊的存在关系最为密切。埃及伊蚊幼虫,而在沃诺索博集装箱周围的照明是主要因素。
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引用次数: 0
Hubungan antara Konsentrasi Minyak Atsiri Kayu Manis (Cinnamomum burmannii Nees Ex Bl.) dalam Lotion dengan Sifat Fisik dan Tingkat Kesukaan Konsumen 肉桂挥发油(朱砂)的挥发油浓度与消费者的生理性质和偏好水平之间的关系
Pub Date : 2017-06-30 DOI: 10.22435/BLB.V13I1.4813.21-28
Erwan Kurnianto, N. Sugihartini, Laela Hayu Nurani
Essential oil of Cinnamomum burmannii contains cinnamaldehyde as active substance as antibacterial and repellant. The study about development of dosage forms was needed in order be useful to people. The purpose of this study was to determine the concentration’s effect of cinnamon to the physical characteristic and results hedonic test of lotion. This study was done in Laboratory of Pharmacy UAD in 2014 that was begun with distillation of essential oil. Then, it was formulated as lotion with concentration 1% (F1), 3% (F II), 5% (F III), 7% (F IV), respectively. The lotion was evaluated its physical characteristic (homogeneity, viscosity, spreadability, adhesivity, pH) and hedonic test to know the level of acceptability based on colour and flavor of lotion. Data was collected  by  quistionnaire. Results of study showed that all of the lotion was homogenous. The increasing concentrations of cinnamon caused decreasing of viscosity (p <0.05) and adhesivity (P <0.05), an increasing of spreadability (P <0.05), did not affect the pH value (p> 0.05), and increasing hedonic level until 5% concentration. It can be concluded that concentration of essential oil of cinnamon at 5% fullfilled the requirement of spreadability, adhesivity, pH  and the most prefered by consumers.
肉桂精油中含有肉桂醛,具有抗菌、驱避的活性物质。为了使其对人类有用,有必要对其剂型的开发进行研究。本研究的目的是确定肉桂的浓度对洗剂的物理特性和结果的影响。本研究于2014年在UAD药房实验室完成,从精油的蒸馏开始。配制成浓度分别为1% (F1)、3% (Fⅱ)、5% (Fⅲ)、7% (Fⅳ)的洗剂。对洗剂的物理特性(均匀性、粘度、涂抹性、粘附性、pH值)进行了评价,并根据洗剂的颜色和风味进行了可接受性测试。数据采用问卷调查方式收集。研究结果表明,所有洗剂均为均匀的。随着肉桂浓度的增加,黏度降低(p 0.05),快感水平升高,直至浓度达到5%。结果表明,5%的肉桂精油浓度可以满足涂布性、黏附性、pH值的要求,是消费者最喜欢的。
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引用次数: 0
Saluran Air Tertutup sebagai Faktor Penekan Populasi Tikus di Daerah Bekas Fokus Pes Cangkringan Sleman Yogyakarta 由于自由聚焦区Sleman Yogyakarta Crescent Pes的老鼠种群压力因素,水道关闭
Pub Date : 2017-06-30 DOI: 10.22435/BLB.V13I1.4557.83-92
Sukismanto Sukismanto, Lupita Chairunnisa, Indah Werdiningsih
Sub-district Cangkringan of Sleman District is an focus area of Plague surveillance at the slopes of Merapi Mountains. Eruption montains was interested alert of rats to insert surrounding villages mountain. Surveillance such as monitoring of rats population was doing for early warning Plague and other disease such as Leptospirosis disease. Sub-district Cangkringan consist of five villages, Wukirsari village is one of former focus. The purpose of study was to determine home environment conditions contributed to trap success. Study design was observational with cross sectional, size of sample as 66 samples (home). Data analyzed with univariat and bivariate. The number of rats caught in the village of Wukirsari are 36 rats with 27.7% trap success with type of rats by Rattus rattus diardi with number of “Xenopsylla cheopis” are 5. Index flea was calculated of 0.13. based on bivariat analysis just closed water channel variabel was significantly correlated with trap success.
Sleman区的Cangkringan街道是默拉皮山脉山坡上瘟疫监测的重点地区。火山爆发引起了人们的兴趣,提醒老鼠将周围的村庄插入山区。监测老鼠数量等监测是为了预警瘟疫和钩端螺旋体病等其他疾病。仓边甘街道由五个村组成,乌基尔萨里村是以前的重点村之一。研究的目的是确定家庭环境条件有助于陷阱的成功。研究设计是观察性的,横截面,样本大小为66个样本(家)。用单变量和双变量分析数据。Wukirsari村捕获的老鼠数量为36只,诱捕成功率为27.7%,腹泻鼠捕获的老鼠类型为5只。跳蚤指数为0.13。基于双变量分析,仅闭水通道变量与诱捕成功率显著相关。
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引用次数: 1
期刊
Balaba Jurnal Litbang Pengendalian Penyakit Bersumber Binatang Banjarnegara
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