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Effect of advanced platelet-rich fibrin applications on periodontal regeneration in infrabony pocket treatment 富血小板纤维蛋白对骨下袋治疗牙周再生的影响
Pub Date : 2019-09-06 DOI: 10.22146/majkedgiind.37414
Christopher Imantaka Suwondo, D. Herawati, S. Sudibyo
One of the regenerative periodontal treatments for infrabony pocket is open flap debridement (OFD) with the addition of growth factor derived from platelet concentrate. Advanced platelet-rich fibrin (A-PRF) is a further development of plateletrich fibrin (PRF) with a lower centrifugation speed (1,500 rpm, 14 minutes). The purpose of this study was to examine the differences in periodontal tissue regeneration after the application of A-PRF and PRF in the treatment of infrabony pockets evaluated from probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The samples were taken from 20 infrabony pockets divided into 2 groups: 10 subjects were treated with OFD+A-PRF and OFD+PRF on the remaining subjects. Probing depth (PD) and relative attachment loss (RAL) measurement were performed on days 0, 30, and 90. Bone height measurements were performed using CBCT X-rays on days 0 and 90. The results showed that PD and RAL reduction in the group of OFD+A-PRF was significantly greater than that in the OFD+PRF group. Bone height reduction in both groups showed no difference. The conclusion obtained from this study is A-PRF application enhances periodontal tissue regeneration by generating greater probing depth and relative attachment loss reduction compared toPRF, as well as an increase in bone height similar to in the treatment of infrabony pockets.
骨下袋的再生牙周治疗方法之一是应用血小板浓缩物衍生的生长因子进行开瓣清创。高级富血小板纤维蛋白(a -PRF)是富血小板纤维蛋白(PRF)的进一步发展,具有较低的离心速度(1500转/分,14分钟)。本研究的目的是研究应用A-PRF和PRF治疗下骨袋后牙周组织再生的差异,从探探深度(PD)、相对附着损失(RAL)和牙槽骨高度来评估。从20个下骨袋中抽取样本,分为2组:10例采用OFD+A-PRF治疗,其余患者采用OFD+PRF治疗。探测深度(PD)和相对附着损失(RAL)测量分别在第0、30和90天进行。在第0天和第90天使用CBCT x射线测量骨高度。结果显示,OFD+A-PRF组PD和RAL的降低明显大于OFD+PRF组。两组骨高降低无明显差异。本研究得出的结论是,与toPRF相比,A-PRF的应用通过产生更大的探探深度和相对的附着损失减少,以及类似于治疗下骨袋的骨高度的增加,促进了牙周组织的再生。
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引用次数: 8
The effect of TiO2 coating and coffee immersion on discoloration of thermoplastic nylon denture base TiO2涂层和咖啡浸泡对热塑性尼龙义齿基托变色的影响
Pub Date : 2019-09-06 DOI: 10.22146/majkedgiind.40045
R. Hidayat, Murti Indrastuti, Heriyanti Amalia Kusuma, Suparyono Saleh
The thermoplastic nylon denture base material is prone to discoloration because its amide bonds absorb water easily. Titanium dioxide (TiO2) nanoparticle have long been used as a denture base coating. Meanwhile, coffee contains chlorogenic and tanic acid, which can change the color of denture bases. The purpose of this study was to examine the effect TiO2 coating and duration of coffee immersion on discoloration of thermoplastic nylon denture base. Samples consisted of 24 thermoplastic nylon in square-shaped (30 x 30 x 2 mm), divided into 4 groups (n = 6). They were control (without TiO2 coating) and treatment (with TiO2 coating) groups, which then were immersed in coffee solution for 15 and 30 days. Discoloration test was conducted using spectrophotometer by measuring the delta absorbance of light before and after coffee immersion. The result showed that the lowest delta absorbance was in the 15-day treatment group (0.011 ± 0.005) and the highest was in the 30-day control group (0.077 ± 0.027). Two-way ANOVA test showed that TiO2 coating and coffee immersion had an effect on discoloration of thermoplastic nylon (p <0.05). Post hoc LSD test showed that there were significant differences between the control and treatment group at 15 and 30 days of coffee immersion (p <0.05). In conclusion, TiO2 as a thermoplastic nylon denture base coating can reduce discoloration by coffee immersion for 15 and 30 days. There were no differences between 15 and 30 days of coffee immersion on thermoplastic nylon’s discoloration in the control and treatment groups.
热塑性尼龙义齿基托材料容易变色,因为它的酰胺键容易吸水。纳米二氧化钛(TiO2)作为义齿基托涂层已被广泛应用。同时,咖啡中含有绿原酸和鞣酸,可以改变义齿基托的颜色。本研究的目的是研究TiO2涂层和咖啡浸泡时间对热塑性尼龙义齿基托变色的影响。样品为24块方形热塑性尼龙(30 × 30 × 2 mm),分为4组(n = 6),分别为对照组(未包覆TiO2)和处理组(包覆TiO2),分别浸泡在咖啡溶液中15和30天。用分光光度计测定咖啡浸泡前后的光的δ吸光度,进行变色试验。结果显示,15 d治疗组δ吸光度最低(0.011±0.005),30 d对照组δ吸光度最高(0.077±0.027)。双因素方差分析表明,TiO2涂层和咖啡浸泡对热塑性尼龙变色有影响(p <0.05)。事后LSD检验显示,在咖啡浸泡15天和30天,对照组和实验组之间存在显著差异(p <0.05)。综上所述,TiO2作为热塑性尼龙义齿基托涂层,咖啡浸泡15天和30天可以减少变色。在咖啡浸泡15天和30天之间,对照组和实验组的热塑性尼龙变色没有差异。
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引用次数: 0
Differences in effectiveness of membrane fixation using periosteal vertical mattress and simple sling suture techniques in infrabony pocket treatment 骨膜垂直垫膜固定与简单吊带缝合治疗下骨袋疗效的差异
Pub Date : 2019-08-31 DOI: 10.22146/majkedgiind.37425
Beta Widya Oktiani, S. Lastianny, Ahmad Syaify
Guide tissue regeneration (GTR) is the treatment of infrabony pockets for soft and hard tissue regeneration. Membrane is used as a barrier and prevents apical migration of the cells in epithelial tissues. Membrane fixation is one of the procedures in GTR treatment because resorbed membrane is less stable. Simple sling suture technique for membrane fixation has 1 anchorage, located in coronal flap, while periosteal vertical mattress suture technique has 2 anchorages in periosteum, making it more stable. This study aimed to study the differences in the effectiveness of membrane fixation using periosteal vertical mattress suture and simple sling suture techniques in terms of probing depth, relative attachment loss, and alveolar bone height in the treatment of infrabony pockets. The samples were divided into 2 groups. The first group was open flap debridement (OFD) with demineralized freeze dried bone allograft (DFDBA) application and membrane fixation with simple sling suture, while the second group was OFD with DFDBA application and membrane fixation with periosteal vertical mattress suture, observed on day 0, 30th day, and 90th day. The results of the study showed significant differences in the probing depth and relative attachment loss (except from the 30th day to the 90th day), and there were no significant differences in the alveolar bone height from the baseline to the 90th day, between the group of membrane fixation using simple sling suture and that of periosteal vertical mattress suture techniques on flap surgery. This study concluded that membrane fixation in the treatment of infrabony pocket with periosteal vertical mattress suture technique is more effective in reducing the probing depth and relative attachment loss, but there is no difference in increasing the alveolar bone height when compared to simple sling suture technique.
引导组织再生(GTR)是一种治疗下骨袋的软硬组织再生方法。在上皮组织中,膜被用作屏障,防止细胞向顶端迁移。膜固定是治疗GTR的一种方法,因为被吸收的膜不太稳定。单纯的悬吊缝合法膜固定有1个锚点,位于冠状皮瓣内,而骨膜垂直床垫缝合法在骨膜内有2个锚点,更加稳定。本研究旨在研究骨膜垂直床垫缝合和简单吊带缝合在治疗下骨袋时,膜固定在探查深度、相对附着丧失和牙槽骨高度方面的差异。将样本分为两组。第一组采用开瓣清创(OFD)联合脱矿冻干同种异体骨移植(DFDBA)应用,单纯吊带缝合膜固定;第二组采用脱矿冻干同种异体骨移植(DFDBA)应用,骨膜垂直床垫缝合膜固定,分别于第0、30、90天进行观察。本研究结果显示,单纯悬吊缝合膜固定组与骨膜垂直垫式缝合瓣手术组的牙槽骨高度从基线到第90天无显著差异(30天至第90天除外),牙槽骨探测深度和相对附着损失均有显著差异。本研究认为膜固定治疗下骨袋与骨膜垂直床垫缝合技术在减少探探深度和相对附着损失方面更有效,但在增加牙槽骨高度方面与单纯吊带缝合技术没有差异。
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引用次数: 0
Effect of salat (prayer) activity on salivary status and cortisol level 祈祷活动对唾液状态和皮质醇水平的影响
Pub Date : 2019-08-31 DOI: 10.22146/majkedgiind.36959
Nadia Rully Auliawati, Layung Sekar Prabarayi, Rais Aliffandy Damroni, Juni Handajani
The majority of Indonesian people are Muslim who perform the obligatory prayers five times each day known as salat. The series of activities in salat begins with wudu with a gargle movement that increases salivary secretion through mechanical stimulation. The movement of ruku and sujood when salat causes oxygen-rich blood to flow to the brain is well suited. When a person feels calmer and more psychically relaxed after performing salat and wudu, the parasympathetic nerves increase so normal cortisol levels can secrete more saliva which is aqueous with normal acidity (pH). Oral health will affect the overall health of the body. This study aimed to determine the effect of salat and wudu on the salivary flow rate, pH, and cortisol level as an indicator of oral health. The design of this study was the static group pretest and posttest design. The study was conducted by comparing the value of salivary flow rate, pH, and cortisol levels in 20 subjects consisting of 10 subjects as the treatment group and 10 subjects as the control. Saliva was collected without stimulation before and after salat activity for five minutes, followed by measurement of salivary flow rate and pH level. Salivary cortisol level was measured using Cortisol Assay Elisa kit with λ 540 nm. The results showed an increase in salivary pH, flow rate and cortisol level after the salat activity but there was no significant difference by Kruskal-Wallis test but there was a strong correlation using Kendall’s tau_b test. In conclusion, salat activity may have correlation with an increase in salivary pH, flow rate and cortisol level.
大多数印尼人是穆斯林,他们每天进行五次强制性的祈祷,称为salat。salat的一系列活动始于wudu,通过机械刺激增加唾液分泌的漱口运动。当睡眠导致富氧血液流向大脑时,ruku和sujood的运动非常适合。当一个人在做salat和wudu之后感到平静和精神放松时,副交感神经会增加,所以正常的皮质醇水平可以分泌更多的唾液,这些唾液的酸度(pH)正常。口腔健康会影响身体的整体健康。本研究旨在确定salat和wudu对唾液流速、pH和皮质醇水平的影响,这是口腔健康的一个指标。本研究采用静态组前测和后测设计。本研究通过比较20名受试者的唾液流速、pH值和皮质醇水平进行研究,其中治疗组10名,对照组10名。在无刺激的情况下,在活动前后5分钟收集唾液,然后测量唾液流速和pH值。采用λ 540 nm的皮质醇测定Elisa试剂盒检测唾液皮质醇水平。结果显示,salat活动后,唾液pH值、流速和皮质醇水平均有所增加,但Kruskal-Wallis检验无显著差异,但Kendall tau_b检验有很强的相关性。综上所述,salat活性可能与唾液pH值、流速和皮质醇水平的升高有关。
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引用次数: 0
Anti-inflammatory role of papaya seed extracts in inhibiting osteoclastogenesis of rats with Periodontitis 木瓜籽提取物抑制牙周炎大鼠破骨细胞生成的抗炎作用
Pub Date : 2019-02-18 DOI: 10.22146/MAJKEDGIIND.26829
Ratih Pusporini, Ahmad Basori, A. Krismariono
Some previous studies mentioned that papaya seed extract has anti-inflammatory activity because it contains polyphenols including phenolic and flavonoid acids. Polyphenols of the papaya seed extracts are known to inhibit the severity of periodontitis. It can prevent the release of excessive osteoclastogenesis which may promote bone resorption aggravating periodontitis. Hence, this study aims to determine the role of papaya seed extract in reducing the number of osteoclasts in rats with periodontitis. Twenty-eight male Wistar rats were divided into 4 groups of induced periodontitis using lipopolisakarida (LPS) Porphyromonas gingivalis (P. Gingivalis) and followed by wire ligature in “8” placed around the mandible mandibular incisor. The first group was given standard food without any papaya extract, while each rat in three other groups with periodontitis (P2, P3, P4) was given with Papaya seed extracts of 200 mg/kgBW, 300 mg/kgBW, and 400 mg/kgBW. Seven rats in one group were not induced by periodontitis and were fed with standard food as a control group. On the 30th day, the rat was sacrificed, and its transverse pieces of alveolar bone around the mandibular incisor were stained with Hematoxylin-Eosin. The data analyzed by one way ANOVA indicated a significant result. Thus, it is conclusive that extract of papaya seed ethanol inhibited osteoclastogenesis in Wistar rats induced with periodontitis.
先前的一些研究提到木瓜籽提取物具有抗炎活性,因为它含有多酚类物质,包括酚类和类黄酮酸。木瓜籽提取物中的多酚被认为可以抑制牙周炎的严重程度。它可以防止释放过多的破骨细胞,从而促进骨吸收,加重牙周炎。因此,本研究旨在确定木瓜籽提取物在减少牙周炎大鼠破骨细胞数量中的作用。将28只雄性Wistar大鼠分为4组,分别用脂质体(LPS)诱导牙龈卟啉单胞菌(P. gingivalis)诱导牙周炎,然后在下颌骨门牙周围用“8”字线结扎。第一组给予不含木瓜提取物的标准食物,其余牙周炎组(P2、P3、P4)给予200 mg/kgBW、300 mg/kgBW、400 mg/kgBW木瓜籽提取物。其中一组7只大鼠未引起牙周炎,饲喂标准食物作为对照组。第30天处死大鼠,用苏木精-伊红染色其下颌切牙周围牙槽骨横片。单因素方差分析的数据显示显著的结果。由此可见,木瓜籽乙醇提取物对牙周炎大鼠破骨细胞生成具有抑制作用。
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引用次数: 4
Effect of Bombyx mori silk-fiber volume on flexural strength of fiber-reinforced composite 家蚕丝纤维体积对纤维增强复合材料抗弯强度的影响
Pub Date : 2019-02-18 DOI: 10.22146/MAJKEDGIIND.25186
Aria Fransiska, S. Sunarintyas, R. Dharmastiti
Dental glass fiber is one of dental synthetic fibers that are widely used in dentistry as a dental resin reinforcement, such as in dentin replacement material. The availability of glass fiber is limited in Indonesia because it must be imported and relatively expensive. Bombyx mori silk-fiber is one of the strongest natural fiber derived from silkworm cocoon processing. Silk-fiber is used in medical applications as a post-surgical sutures, scaffolds for tissue engineering and drug delivery. The purpose of this study was to evaluate the effect of Bombyx mori silk-fiber volume on the flexural strength of fiber-reinforced composite (FRC). We used Bombyx mori silk-fiber (Perhutani Pati, Central Java, Indonesia) and flowable composite (Charmfil flow, Denkist, Korea) in this study. The FRC samples were divided into 4 groups consisting of fiber volumes of 0%, 5%, 10% and 15% (n = 4). Tests of flexural strength were performed according to ISO 4049. The results were analyzed using one way ANOVA (p<0.05). The study showed that the means of the flexural strength (MPa) of Bombyx mori silk-fiber FRC for volume of 0%, 5%, 10% and 15% were 149.2 ± 5.5; 127.6 ± 3.8; 110.9 ± 3.5; 71.2 ± 4.2. One-way ANOVA test showed that the means of FRC flexural strength on the four groups’ silk-fiber Bombyx mori volumetric were significantly different (p<0.05). This study concluded that Bombyx mori silk-fiber volumetric influences the flexural strength of fiber- reinforced composite. An increase in Bombyx mori silk-fiber volume decreases the flexural strength of FRC because there is a small gap due to the weak interfacial bonds between dental flowable composite and Bombyx mori silk-silk-fiber.
牙科玻璃纤维是牙科合成纤维的一种,广泛用于牙科树脂增强,如牙本质替代材料。在印度尼西亚,玻璃纤维的供应有限,因为它必须进口,而且相对昂贵。家蚕丝纤维是蚕茧加工过程中提取的最坚固的天然纤维之一。丝纤维在医学上被用作手术后缝合线、组织工程支架和药物输送。研究家蚕丝纤维体积对纤维增强复合材料(FRC)抗弯强度的影响。本研究使用家蚕丝纤维(Perhutani Pati,中爪哇,印度尼西亚)和可流动复合材料(Charmfil flow, Denkist,韩国)。FRC样品分为纤维体积为0%、5%、10%和15%的4组(n = 4),抗弯强度测试按照ISO 4049进行。结果采用单因素方差分析(p<0.05)。研究表明,家蚕丝纤维FRC在体积为0%、5%、10%和15%时的抗弯强度平均值为149.2±5.5;127.6±3.8;110.9±3.5;71.2±4.2。单因素方差分析表明,FRC抗弯强度均值对四组蚕丝家蚕体积有显著差异(p<0.05)。本研究认为家蚕丝纤维体积对纤维增强复合材料的抗弯强度有影响。家蚕丝纤维体积的增加会降低FRC的抗弯强度,这是因为牙流复合材料与家蚕丝-丝纤维之间的界面结合较弱,存在较小的间隙。
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引用次数: 2
Evaluation of surface roughness of the bracket slot before and after burning 燃烧前后支架槽的表面粗糙度评价
Pub Date : 2019-02-18 DOI: 10.22146/MAJKEDGIIND.28387
R. Jannah, B. Thahar, Endah Mardiati, I. A. Evangelina
Slot bracket base is one important element to enable sliding movement of the archwire. Bracket reconditioning by means of burning changes the surface roughness of slot bracket base. The heat above 450 °C shows microstructure changes of the slot surface. On this basis, the present study aims to evaluate surface roughness changes of the slot bracket base before and after reconditioning by means of burning. Sixteen premolar brackets standard edgewise were divided into two groups each consisting of eight brackets. These two groups were tested at three different points using Scanning Electron Microscopy (SEM), with 3000 times of magnification. Subsequentl,group A was burned for 5 seconds (approximately 450 °C) and group B was burned for 10 seconds(approximately 643 °C). After treatment,these two groups were tested for the second time using Scanning Electron Microscopy (SEM), with 3000 times magnification and the images were analyzed using a discrete scale quantitative classification. Afterwards, the data were analyzed using ANOVA (p≤0.05) and post hoc analysis. The result showed that surface roughness of the slot bracket base burned for 10 seconds experienced more significant change than that of bracket burned for 5 seconds and that of the control bracket(p≤0.05).Bracket reconditioning using burning either for 5 to 10 seconds change the surface roughness of the slot bracket base.
槽式支架底座是保证拱丝滑动运动的重要元件。燃烧修复支架会改变槽式支架底座的表面粗糙度。在450℃以上加热时,狭槽表面出现了显微组织变化。在此基础上,本研究旨在评价槽式支架底座燃烧修复前后的表面粗糙度变化。16个标准的前磨牙托槽分为两组,每组8个托槽。使用放大3000倍的扫描电子显微镜(SEM)在三个不同的点对这两组进行测试。随后,A组烧伤5秒(约450°C), B组烧伤10秒(约643°C)。治疗后,对两组进行第二次扫描电镜(SEM)检测,扫描电镜放大3000倍,图像采用离散尺度定量分类分析。之后,采用方差分析(p≤0.05)和事后分析对数据进行分析。结果表明,燃烧10秒槽型支架底座表面粗糙度的变化比燃烧5秒槽型支架和对照槽型支架表面粗糙度的变化更显著(p≤0.05)。支架修复使用燃烧5到10秒改变槽支架底座的表面粗糙度。
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引用次数: 1
The effect of silk concentration on the flexural strength of FRC as a Bone Graft 丝浓度对FRC骨移植物抗弯强度的影响
Pub Date : 2019-02-18 DOI: 10.22146/MAJKEDGIIND.31487
Rifki Moechtar, S. Sunarintyas, M. K. Herliansyah
Bone fractures are mostly caused by trauma and disease. In the therapeutic process of bone healing which often meets some constraints, bone graft is mainly used to ensure that the healing process takes place. A fiber reinforced composite (FRC) is a popular bone graft material that is made to resemble bone properties. FRC is normally comprised of polymer matrix, hydroxyapatite filler, and fiber. Hydroxyapatite is a bioactive material widely used as a bone graft. Silk fiber is known as a reliable material to increase mechanical strength of the FRC. On this basis, this study aims to determine the effect of silk fiber concentration on the flexural strength of FRC. Fiber reinforced composite made of Bis-GMA/TEGDMA/ UDMA resin (CharmFil®, DenKist, Korea), hydroxyapatite (Bioceramic Laboratory, DTMI UGM) and silk fiber (Perhutani, Pati) were divided into three groups. Each group contained different silk fiber concentrations which were 1%, 5% and 10%. The flexural strength test was performed with 3-point bending test according to ISO 10477. The result showed that FRC with silk fiber 1%, 5% and 10% respectively had flexural strength of 61.21 ± 8.43 MPa, 62.97 ± 3.92 MPa and 85.01 ± 7.71 MPa. The result of one way ANAVA analysis showed that mean of FRCs flexural strength were significantly different between one treatment group to another. Thus, it is conclusive that silk fiber has a significant effect on FRCs flexural strength. The addition of 10% concentration of silk fiber is proven to increase FRCs flexural strength.
骨折多由外伤和疾病引起。在骨愈合的治疗过程中往往会遇到一些限制,骨移植主要用于保证愈合过程的发生。纤维增强复合材料(FRC)是一种流行的骨移植材料,它被制成类似骨的特性。FRC通常由聚合物基体、羟基磷灰石填料和纤维组成。羟基磷灰石是一种生物活性材料,广泛应用于骨移植。蚕丝纤维被认为是提高纤维纤维机械强度的可靠材料。在此基础上,本研究旨在确定丝纤维浓度对FRC抗弯强度的影响。由Bis-GMA/TEGDMA/ UDMA树脂(CharmFil®,DenKist, Korea)、羟基磷灰石(biocceramic Laboratory, DTMI UGM)和丝纤维(Perhutani, Pati)组成的纤维增强复合材料分为三组。各组蚕丝纤维浓度分别为1%、5%和10%。弯曲强度试验采用ISO 10477标准的三点弯曲试验。结果表明,丝纤维含量分别为1%、5%和10%的FRC抗弯强度分别为61.21±8.43 MPa、62.97±3.92 MPa和85.01±7.71 MPa。单向ANAVA分析结果显示,两组间FRCs抗弯强度均值差异显著。由此可见,丝纤维对FRCs抗弯强度有显著影响。结果表明,添加10%浓度的丝纤维可提高纤维纤维的抗弯强度。
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引用次数: 0
Streamline characteristics using the computational fluid dynamic analysis in the flow of 18% EDTA irrigation solution to remove Ca(OH)2 利用计算流体动力学分析流线特性在18% EDTA灌洗液中去除Ca(OH)2
Pub Date : 2019-02-18 DOI: 10.22146/MAJKEDGIIND.30886
A. Muryani, Hendra Dian Adhita Dharsono, Z. Zuleika, I. M. A. Moelyadi, D. Prisinda
The remaining calcium hydroxide (Ca(OH)2) medicaments in the root canal wall can block the penetration of filling medicaments to the dentine tubule and cause the failure in the root canal treatment. One of the ways in cleaning the root canal wall from the remains of (Ca(OH)2) is by using 18% Ethylene Diamine Tetra Acetic Acid (EDTA) irrigation solution. The cleanliness of the root canal can be examined using the Computational Fluid Dynamics (CFD) analysis. The aim of this research was to see the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with 18% EDTA irrigation by means of CFD analysis. This is a descriptive-explorative research. Having been validated with the experimental research using test specimen in the form of block resin made in accordance with the characteristics of root canal, the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with the 18% EDTA irrigation was analyzed using CFD method. The irrigation needle used was the side-vented type with the position of 3 mm from the tooth apical. The results of the research showed the conformity between the result of experimental research and CFD research. One of the results of this research was the characteristics of streamline of 18% EDTA in the root canal showing a unique behavior due to the characteristics of the form of side vent irrigation needle. The irrigation flow in the coronal area of the inlet (side vent irrigation needle) showed a low velocity causing the more fluid flowing out from the inlet went to apical rather to the outlet (root canal orifice). In conclusion, this research showed that 18% EDTA solution indicated the conformity of validation results between experimental research and CFD research in the frames of 5, 10, 15, and 20 secs observed from experimental research with the of frame 0,010, 0,099, 0,150 and 0,410 secs as observed from CFD research.
根管壁上残留的氢氧化钙(Ca(OH)2)药物会阻碍填充药物向牙本质小管渗透,导致根管治疗失败。清除(Ca(OH)2)残留根管壁的方法之一是使用18%乙二胺四乙酸(EDTA)冲洗液。使用计算流体动力学(CFD)分析可以检查根管的清洁度。本研究的目的是通过CFD分析,观察(Ca(OH)2)药物在18% EDTA灌洗下对根管壁清洁度的描述。这是一项描述性探索性研究。采用根据根管特性制作的块状树脂试样进行实验研究验证后,采用CFD方法对(Ca(OH)2)药剂在18% EDTA灌洗下对根管壁清洁度的描述进行了分析。冲洗针为侧通气型,距牙尖3mm。研究结果表明了实验研究结果与CFD研究结果的一致性。本研究的结果之一是18% EDTA在根管内流线的特征,由于侧孔灌针形式的特点,呈现出独特的行为。入口冠状区(侧排灌针)的灌流速度较慢,导致从入口流出的液体更多地流向根尖而不是出口(根管口)。综上所述,本研究表明,18%的EDTA溶液表明,实验研究观察到的5、10、15和20秒帧与CFD研究观察到的0,010、0,099、0,150和0,410秒帧的验证结果与实验研究的验证结果一致。
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引用次数: 0
Inhibition activity of Robusta coffee beans polyphenol extract on the production of TNF-α neutrophil cells 罗布斯塔咖啡豆多酚提取物对TNF-α中性粒细胞生成的抑制作用
Pub Date : 2019-02-18 DOI: 10.22146/MAJKEDGIIND.28352
T. Ermawati, Z. Meilawaty, Happy Harmono
Polyphenols are one of the active substances in the Robusta coffee beans with various benefits for humans’ health including anti-inflammation. neutrophil cell (polymorphonuclear PMN) plays a significant role as the primary immune response against foreign agent. Inflammatory response is characterized by the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α). Hence, the purpose of this study is to determine anti-inflammation capacity of Robusta coffee beans polyphenols extract on the TNF-α production in neutrophil cells. Neutrophil was derived from human peripheral venous blood by means of gradient density method. One hundred microliter of neutrophil  (1,5x103 cell) was incubated with 3.13%, 6.25%, 12.5%, 25% polyphenol extract and exposed with 100 μl of Lipopolysaccharides (LPS) 25 mg/ml. The production of TNF-α were determined by ELISA. Data were analyzed by one way Anova. Polyphenols of Robusta coffee bean extract was shown to inhibit TNF-α production in LPS-exposed neutrophil cells compared to controls. Whereas, LPS-exposed neutrophil increased TNF-α production. The most effective concentration to inhibit TNF-α production was 12.5%. It was concluded that polyphenols of Robusta coffee beans has anti-inflammatory properties as indicated by its ability to decrease TNF-α levels.
多酚是罗布斯塔咖啡豆中的活性物质之一,对人体健康有多种益处,包括抗炎症。中性粒细胞(多形核PMN)在抵抗外来物的初级免疫应答中起着重要作用。炎症反应的特征是产生促炎细胞因子,如肿瘤坏死因子-α (TNF-α)。因此,本研究的目的是确定罗布斯塔咖啡豆多酚提取物对中性粒细胞中TNF-α产生的抗炎能力。用梯度密度法从人外周静脉血中提取中性粒细胞。中性粒细胞100微升(1,5 × 103细胞)与3.13%、6.25%、12.5%、25%多酚提取物孵育,并与100 μl脂多糖(LPS) 25 mg/ml接触。ELISA法检测TNF-α的产生。数据采用单因素方差分析。与对照组相比,罗布斯塔咖啡豆提取物的多酚被证明可以抑制lps暴露的中性粒细胞中TNF-α的产生。然而,lps暴露的中性粒细胞增加了TNF-α的产生。抑制TNF-α产生的最有效浓度为12.5%。结果表明,罗布斯塔咖啡豆多酚具有抗炎作用,可降低肿瘤坏死因子-α水平。
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引用次数: 1
期刊
Majalah Kedokteran Gigi Indonesia
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