Nicholas C. Jones, R. Nazarian, L. Duncan, D. Wilbur
Nonsubspecialized pathologists frequently request expert consultation in challenging dermatopathology cases. Traditional consultation practice utilizing shipment of glass slides is costly, slow, and of limited educational benefit to the referring physician. Whole slide imaging (WSI) has been suggested as a potential method of overcoming these limitations in the current glass slide consultation practice, but there have been concerns regarding the adequacy of image quality for interpretation of challenging dermatopathology cases. We aimed to investigate the performance of WSI in challenging dermatopathology consult cases.
{"title":"MGH Whole Slide Imaging Teleconsultation Practice in Dermatopathology","authors":"Nicholas C. Jones, R. Nazarian, L. Duncan, D. Wilbur","doi":"10.1155/2014/347147","DOIUrl":"https://doi.org/10.1155/2014/347147","url":null,"abstract":"Nonsubspecialized pathologists frequently request expert consultation in challenging dermatopathology cases. Traditional consultation practice utilizing shipment of glass slides is costly, slow, and of limited educational benefit to the referring physician. Whole slide imaging (WSI) has been suggested as a potential method of overcoming these limitations in the current glass slide consultation practice, but there have been concerns regarding the adequacy of image quality for interpretation of challenging dermatopathology cases. We aimed to investigate the performance of WSI in challenging dermatopathology consult cases.","PeriodicalId":313227,"journal":{"name":"Analytical Cellular Pathology (Amsterdam)","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125733040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Brachtel, Barbara L. Smith, G. Tearney, Dongkyun Kang
Complete removal of breast cancer during a single breastconserving lumpectomy procedure is often challenging due to the lack of adequate intraoperative tools to accurately determine the margin status. About one-third of lumpectomy patients are found to have positive margins upon final histologic analysis, which usually is reported within a week after surgery. These patients are then required to undergo additional surgeries, which increases the patient morbidity, cosmetic challenges, and healthcare cost. Spectrally encoded confocal microscopy (SECM) is a high-speed confocal microscopy technique [1] that can visualize cellular and subcellular features of an unstained fresh tissue. SECM is 10–100 times faster than conventional confocal microscopes and has been demonstrated to image an entire endoscopic mucosal resection (EMR) esophageal tissue (10mm by 10mm) within 15 seconds [2]. The high imaging speed of SECM may make it possible to rapidly image the margins of entire lumpectomy specimens to comprehensively determine margin status without sampling error. Real-time feedback regarding the margin status could enable the surgeon to achievemore thorough tumor removal in a single surgery and will significantly reduce the need for additional surgeries.The aim of this preliminary studywas to test SECM for visualizing breast cancers with various morphologic features.
{"title":"Spectrally Encoded Confocal Microscopy for Guiding Lumpectomy","authors":"E. Brachtel, Barbara L. Smith, G. Tearney, Dongkyun Kang","doi":"10.1155/2014/573851","DOIUrl":"https://doi.org/10.1155/2014/573851","url":null,"abstract":"Complete removal of breast cancer during a single breastconserving lumpectomy procedure is often challenging due to the lack of adequate intraoperative tools to accurately determine the margin status. About one-third of lumpectomy patients are found to have positive margins upon final histologic analysis, which usually is reported within a week after surgery. These patients are then required to undergo additional surgeries, which increases the patient morbidity, cosmetic challenges, and healthcare cost. Spectrally encoded confocal microscopy (SECM) is a high-speed confocal microscopy technique [1] that can visualize cellular and subcellular features of an unstained fresh tissue. SECM is 10–100 times faster than conventional confocal microscopes and has been demonstrated to image an entire endoscopic mucosal resection (EMR) esophageal tissue (10mm by 10mm) within 15 seconds [2]. The high imaging speed of SECM may make it possible to rapidly image the margins of entire lumpectomy specimens to comprehensively determine margin status without sampling error. Real-time feedback regarding the margin status could enable the surgeon to achievemore thorough tumor removal in a single surgery and will significantly reduce the need for additional surgeries.The aim of this preliminary studywas to test SECM for visualizing breast cancers with various morphologic features.","PeriodicalId":313227,"journal":{"name":"Analytical Cellular Pathology (Amsterdam)","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126483182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Today’s gold standard for medical diagnosis is histology of excised biopsies or surgical specimens where tissue is taken out of the body, processed, sectioned, stained, and looked at under a light microscope by a pathologist. There are many limitations of this technique, including the fact that it is inherently invasive, time consuming, costly, and dangerous for some organs. Furthermore, oftentimes the diseased tissue is not readily seen by visual inspection and as a result the tissue is sampled at a random location, which can be highly inaccurate. If we could instead conduct microscopy inside the body, then we could provide tools for screening, targeting biopsies, making primary disease diagnosis, and guiding intervention on the cellular basis. This promise has motivated the development of a new field, termed in vivo microscopy, the goal of which is to obtain microscopic images from living human patients. Two in vivo microscopy technologies, confocal microscopy and optical coherence tomography, are currently available and in clinical use. Upcoming developments, including whole organ microscopy, swallowable microscopy capsules, molecular imaging, and very high resolution microscopic devices, are in the pipeline and will likely revolutionize how disease is diagnosed and how medicine is practiced in the future.
{"title":"Microimaging: Seeing the Unseen in Living Patients","authors":"G. Tearney","doi":"10.1155/2014/797108","DOIUrl":"https://doi.org/10.1155/2014/797108","url":null,"abstract":"Today’s gold standard for medical diagnosis is histology of excised biopsies or surgical specimens where tissue is taken out of the body, processed, sectioned, stained, and looked at under a light microscope by a pathologist. There are many limitations of this technique, including the fact that it is inherently invasive, time consuming, costly, and dangerous for some organs. Furthermore, oftentimes the diseased tissue is not readily seen by visual inspection and as a result the tissue is sampled at a random location, which can be highly inaccurate. If we could instead conduct microscopy inside the body, then we could provide tools for screening, targeting biopsies, making primary disease diagnosis, and guiding intervention on the cellular basis. This promise has motivated the development of a new field, termed in vivo microscopy, the goal of which is to obtain microscopic images from living human patients. Two in vivo microscopy technologies, confocal microscopy and optical coherence tomography, are currently available and in clinical use. Upcoming developments, including whole organ microscopy, swallowable microscopy capsules, molecular imaging, and very high resolution microscopic devices, are in the pipeline and will likely revolutionize how disease is diagnosed and how medicine is practiced in the future.","PeriodicalId":313227,"journal":{"name":"Analytical Cellular Pathology (Amsterdam)","volume":"371 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114869858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microarray Core Detection by Geometric Restoration","authors":"Jimmy C. Azar, C. Busch, I. Carlbom","doi":"10.1155/2013/391535","DOIUrl":"https://doi.org/10.1155/2013/391535","url":null,"abstract":"","PeriodicalId":313227,"journal":{"name":"Analytical Cellular Pathology (Amsterdam)","volume":"146 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115241359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
s of the 1st Congress of the International Academy of Digital Pathology August 3-5, 2011 Quebec city, Canada The challenges of implementing a “patientoriented” telepathology network; the Eastern Quebec telepathology project experience
{"title":"Abstracts of the 1st Congress of the International Academy of Digital Pathology August 3–5, 2011 Quebec city, Canada","authors":"B. Têtu","doi":"10.3233/ACP-2011-0020","DOIUrl":"https://doi.org/10.3233/ACP-2011-0020","url":null,"abstract":"s of the 1st Congress of the International Academy of Digital Pathology August 3-5, 2011 Quebec city, Canada The challenges of implementing a “patientoriented” telepathology network; the Eastern Quebec telepathology project experience","PeriodicalId":313227,"journal":{"name":"Analytical Cellular Pathology (Amsterdam)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128075006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jason P. Hipp, S. C. Smith, Jerome Cheng, S. Tomlins, J. Monaco, A. Madabhushi, P. Kunju, U. Balis
s of the 1st Congress of the International Academy of Digital Pathology: Poster Session 199 Medical School Pathology education supplemented with web-based virtual microscopy Rajyasree Emmadi, Amy Y. Lin, and Andy V. Pham Department of Pathology, University of Illinois at Chicago (UIC), IL, USA Background: The year 2 Medical student Pathology practicum at UIC, designated Small Group Discussion (SGD), comprises 184 students in groups of 14–16 each. Traditionally the practicum has involved students examining and learning morphologic pathology by viewing glass slides through the microscope, with accompanying instruction, in 2-hour sessions. We decided to introduce web-based virtual microscopy to the SGDs to augment teaching and facilitate self-paced student learning. Methods: Selected glass slides were scanned using the Aperio ScanScope (Aperio, Vista, CA), to obtain the virtual slide images and interactive cases were created using Digital Slide Box (DSB) software (SlidePath, Dublin, Ireland). Each virtual slide was annotated with key microscopic features of the case. Each case, in turn, was accompanied by a narrative, which included a clinical history, physical examination fi ndings, and gross and microscopic descriptions. A total of 39 virtual slide sets were utilized for the current academic year. Hyperlinks within the narrative are available to integrate clinical photographs, gross photographs, imaging studies, multimedia, additional virtual slides or slide annotations to the case. At the end of each module, self-assessment quizzes helped students test their understanding and identify individual weaknesses. Results: These interactive, web-based virtual microscopy cases were provided to the M2 students the week prior to the actual SGD. This allowed the students to study the material at leisure and without a time constraint. At the actual SDG, the students were able to concurrently view different glass slides of the specifi c disease process as well as re-review the online material. While initially the in-class bandwidth slowed the online review noticeably, this was resolved by effecting a change in the DSB server confi guration. An informal feedback showed that the students were very receptive to this new technology and found these selfstudy cases useful in increasing their recognition and understanding of pathologic processes in diseases. They particularly liked the ‘anytime, anywhere’ access and the ability to dispense with the microscope. Conclusions: An interactive, web-based virtual microscopy case study set makes Pathology more accessible and inviting to Medical students. In the next academic year we plan to expand the virtual microscopy content. Toward an annotated digital Multiphoton Microscopy (MPM) histology atlas of fresh human bladder biopsies for intra-cystoscopy guidance in bladder cancer diagnosis Sushmita Mukherjee, Manu Jain, Brian D. Robinson, Joshua Sterling, Douglas S. Scherr, Bekheit Salmoon, Frederick R. Maxfi eld, Warren R.
Rajyasree Emmadi, Amy Y. Lin, and Andy V. Pham伊利诺伊大学芝加哥分校病理系，伊利诺伊州，美国背景:伊利诺伊大学芝加哥分校二年级的医学生病理学实习，被称为小组讨论(SGD)，由184名学生组成，每组14-16人。传统上，学生在两个小时的课程中通过显微镜观察玻片来检查和学习形态病理学，并辅以指导。我们决定向SGDs引入基于网络的虚拟显微镜，以增强教学并促进学生自主学习。方法:选用Aperio ScanScope (Aperio, Vista, CA)对选定的玻片进行扫描，获得虚拟玻片图像，并使用Digital slide Box (DSB)软件(SlidePath, Dublin, Ireland)创建交互式病例。每张虚拟幻灯片都标注了病例的关键显微特征。每个病例依次附有一份叙述，其中包括临床病史、体格检查结果以及大体和显微镜下的描述。本学年共使用了39套虚拟幻灯片。叙述中的超链接可用于整合临床照片，大体照片，影像学研究，多媒体，额外的虚拟幻灯片或幻灯片注释。在每个模块的最后，自我评估测验帮助学生测试他们的理解和识别个人的弱点。结果:这些交互式的、基于网络的虚拟显微镜病例在实际SGD前一周提供给M2学生。这使得学生可以轻松地学习材料，而不受时间限制。在实际的可持续发展目标中，学生们能够同时观看特定疾病过程的不同玻片，并重新审查在线材料。虽然最初的类内带宽明显减慢了在线评论，但这是通过影响DSB服务器配置的变化来解决的。一个非正式的反馈表明，学生们非常接受这种新技术，并发现这些自学案例有助于增加他们对疾病病理过程的认识和理解。他们特别喜欢“随时随地”的访问和无需显微镜的能力。结论:一个交互式的，基于网络的虚拟显微镜案例研究集使病理学更容易接近和吸引医学生。在下一学年，我们计划扩展虚拟显微镜的内容。新膀胱活检的数字多光子显微镜(MPM)组织图用于膀胱内镜检查指导膀胱癌诊断Sushmita Mukherjee, Manu Jain, Brian D. Robinson, Joshua Sterling, Douglas S. Scherr, Bekheit Salmoon, Frederick R. Maxfi field, Warren R. Zipfel和Watt W. Webb美国3威尔康奈尔医学院病理与检验医学系，纽约，纽约，美国4康奈尔大学生物医学工程系，伊萨卡，纽约，美国5康奈尔大学应用与工程物理学院，伊萨卡，纽约，美国背景:从福尔马林固定标本中获得的苏木精和伊红(H&E)染色切片是目前组织病理学诊断的金标准。虽然这些方法高度可靠，但它们需要很长时间(处理、切片、染色和病理学家的阅读)。尽管数字化玻片的工作正在进行中，但大多数病理学家仍然阅读模拟玻片;因此，自动形态测量和实时在线咨询是罕见的。此外，组织学切片的二维性质排除了不耗时连续切片的三维组织结构评估。方法:多光子显微镜(MPM)是一种非线性成像技术，可以在亚细胞分辨率和组织表面以下0.5 mm深度下生成组织的三维组织学。这使得基于光谱分辨组织发射(ITE)信号的新鲜(未固定、未切片和未染色)组织几乎可以即时成像:(1)细胞质成分的自动荧光和国际数字病理学学会第一届大会的200篇摘要:海报会议弹性蛋白纤维;(2)二次谐波产生(SHG)，这是胶原束和定向微管的非线性散射信号。使用单一激发波长和使用波长带通滤波器收集发射信号，SHG和各种自动荧光成分可以单独获取，分析和颜色编码，以便于可视化。 乳腺组织对人表皮生长因子re的免疫染色显示了保留的抗原性
{"title":"Poster Session","authors":"Jason P. Hipp, S. C. Smith, Jerome Cheng, S. Tomlins, J. Monaco, A. Madabhushi, P. Kunju, U. Balis","doi":"10.3233/ACP-2011-0021","DOIUrl":"https://doi.org/10.3233/ACP-2011-0021","url":null,"abstract":"s of the 1st Congress of the International Academy of Digital Pathology: Poster Session 199 Medical School Pathology education supplemented with web-based virtual microscopy Rajyasree Emmadi, Amy Y. Lin, and Andy V. Pham Department of Pathology, University of Illinois at Chicago (UIC), IL, USA Background: The year 2 Medical student Pathology practicum at UIC, designated Small Group Discussion (SGD), comprises 184 students in groups of 14–16 each. Traditionally the practicum has involved students examining and learning morphologic pathology by viewing glass slides through the microscope, with accompanying instruction, in 2-hour sessions. We decided to introduce web-based virtual microscopy to the SGDs to augment teaching and facilitate self-paced student learning. Methods: Selected glass slides were scanned using the Aperio ScanScope (Aperio, Vista, CA), to obtain the virtual slide images and interactive cases were created using Digital Slide Box (DSB) software (SlidePath, Dublin, Ireland). Each virtual slide was annotated with key microscopic features of the case. Each case, in turn, was accompanied by a narrative, which included a clinical history, physical examination fi ndings, and gross and microscopic descriptions. A total of 39 virtual slide sets were utilized for the current academic year. Hyperlinks within the narrative are available to integrate clinical photographs, gross photographs, imaging studies, multimedia, additional virtual slides or slide annotations to the case. At the end of each module, self-assessment quizzes helped students test their understanding and identify individual weaknesses. Results: These interactive, web-based virtual microscopy cases were provided to the M2 students the week prior to the actual SGD. This allowed the students to study the material at leisure and without a time constraint. At the actual SDG, the students were able to concurrently view different glass slides of the specifi c disease process as well as re-review the online material. While initially the in-class bandwidth slowed the online review noticeably, this was resolved by effecting a change in the DSB server confi guration. An informal feedback showed that the students were very receptive to this new technology and found these selfstudy cases useful in increasing their recognition and understanding of pathologic processes in diseases. They particularly liked the ‘anytime, anywhere’ access and the ability to dispense with the microscope. Conclusions: An interactive, web-based virtual microscopy case study set makes Pathology more accessible and inviting to Medical students. In the next academic year we plan to expand the virtual microscopy content. Toward an annotated digital Multiphoton Microscopy (MPM) histology atlas of fresh human bladder biopsies for intra-cystoscopy guidance in bladder cancer diagnosis Sushmita Mukherjee, Manu Jain, Brian D. Robinson, Joshua Sterling, Douglas S. Scherr, Bekheit Salmoon, Frederick R. Maxfi eld, Warren R. ","PeriodicalId":313227,"journal":{"name":"Analytical Cellular Pathology (Amsterdam)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115891174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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