Background: The chemotherapeutic use of cisplatin (CP) is restricted because of its hepatotoxicity induced by oxidative stress. Malondialdehyde (MDA) is a secondary product of lipid peroxidation as a biomarker of oxidative stress. Individual administration of black seed oil (BSO) or honey (H) demonstrated hepatoprotective effect in rats. Interaction of both substances when administrated as combination can be evaluated using combination index (CI) to quantitatively depict synergism (CI<1), additive (CI=1) and antagonism effect (CI>1). Objective: to know the combination effect of BSO and honey on rat liver tissue given CP exposure. Methods: This study used 30 rats were divided into 10 groups. Normal group (N); Negative control group (NC); P1-P4 groups were administerated BSO (1 and 2 mL/kg) and honey (3.7 and 7.4 mL/kg); P5-P8 groups were combination of BSO and H. P1-P8 groups were given BSO and honey orally for 21 days. On the 18th day, NC and P1-P8 groups were given CP 8 mg/kg intraperitoneally, while the N group was given NaCl 0.9% 1 mL/kg intraperitoneally. Result: Malondialdehyde (MDA) levels were found to be lower in P1-P8 groups compared to negative control group and P6 and P7 groups have levels equivalent to MDA levels of normal control group (p > 0.05). Conclusion: Combination of BSO and honey provides a protective effect on cisplatin-induced rat liver tissue damage indicated by reduced MDA levels, but all combination group showed antagonism effect.
{"title":"Protective Effect of Combination Commercial Black Seed Oil (Nigella sativa) and Honey Against Cisplatin-Induced Hepatotoxicity in Rats","authors":"A. Siddiq, M. I. Ilmiawan, Mitra Handini","doi":"10.24853/mmj.1.2.1-6","DOIUrl":"https://doi.org/10.24853/mmj.1.2.1-6","url":null,"abstract":"Background: The chemotherapeutic use of cisplatin (CP) is restricted because of its hepatotoxicity induced by oxidative stress. Malondialdehyde (MDA) is a secondary product of lipid peroxidation as a biomarker of oxidative stress. Individual administration of black seed oil (BSO) or honey (H) demonstrated hepatoprotective effect in rats. Interaction of both substances when administrated as combination can be evaluated using combination index (CI) to quantitatively depict synergism (CI<1), additive (CI=1) and antagonism effect (CI>1). Objective: to know the combination effect of BSO and honey on rat liver tissue given CP exposure. Methods: This study used 30 rats were divided into 10 groups. Normal group (N); Negative control group (NC); P1-P4 groups were administerated BSO (1 and 2 mL/kg) and honey (3.7 and 7.4 mL/kg); P5-P8 groups were combination of BSO and H. P1-P8 groups were given BSO and honey orally for 21 days. On the 18th day, NC and P1-P8 groups were given CP 8 mg/kg intraperitoneally, while the N group was given NaCl 0.9% 1 mL/kg intraperitoneally. Result: Malondialdehyde (MDA) levels were found to be lower in P1-P8 groups compared to negative control group and P6 and P7 groups have levels equivalent to MDA levels of normal control group (p > 0.05). Conclusion: Combination of BSO and honey provides a protective effect on cisplatin-induced rat liver tissue damage indicated by reduced MDA levels, but all combination group showed antagonism effect.","PeriodicalId":32215,"journal":{"name":"Journal of Muhammadiyah Medical Laboratory Technologist","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84249196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Dyslipidemia is a condition where there is an abnormal lipid profile in the blood. Modern patterns and lifestyles such as consuming fast food cause excessive fat consumption which can increase the possibility of contracting blood vessel and heart disease. High levels of High Density Lipoprotein (HDL) cholesterol can protect the body against heart disease and stroke, but not many drugs can increase HDL cholesterol levels. Olive oil is an oil that contains 77% Mono Unsaturated Fatty Acid (MUFA) which can reduce LDL cholesterol and increase HDL cholesterol. Objective: To determine the effect of olive oil (Extra Virgine Olive Oil-EVOO) and moderate intensity exercise on high density lipoprotein (HDL) levels in male Wistar strain (Rattus novergicus L) rats fed a high-fat diet. Method: a true experimental research design with a pretest and posttest design with a control group (Pretest and Posttest with Control Group) with simple randomization. Results: There was an increase in HDL levels in the control group (42.50 to 44.50 mg / dl), the olive oil treatment group was (50.83 to 53.33) mg / dL, the moderate intensity exercise treatment group (46.67 to 52 , 00 mg / dl), and the moderate-intensity exercise and olive oil treatment groups (47.50 to 58.67 mg / dl). Conclusion: Extra Virgine Olive Oil, moderate intensity exercise, and Extra Virgine Olive Oil with moderate intensity exercise influence the increase of HDL levels in the blood of male Wistar strain rats fed a high-fatty diet.
{"title":"The Effect of Olive Oil (Extra Virgine Olive Oil) and Moderate-Intensity Exercise On The Level of High Density Lipoprotein (HDL) in Male Wistar Strain Rat (Rattus novergicus L) Which Was Given High Fatty Diet","authors":"Ikhsan Syakban Anwari, Hendra Sutysna","doi":"10.24853/mmj.1.2.27-34","DOIUrl":"https://doi.org/10.24853/mmj.1.2.27-34","url":null,"abstract":"Background: Dyslipidemia is a condition where there is an abnormal lipid profile in the blood. Modern patterns and lifestyles such as consuming fast food cause excessive fat consumption which can increase the possibility of contracting blood vessel and heart disease. High levels of High Density Lipoprotein (HDL) cholesterol can protect the body against heart disease and stroke, but not many drugs can increase HDL cholesterol levels. Olive oil is an oil that contains 77% Mono Unsaturated Fatty Acid (MUFA) which can reduce LDL cholesterol and increase HDL cholesterol. Objective: To determine the effect of olive oil (Extra Virgine Olive Oil-EVOO) and moderate intensity exercise on high density lipoprotein (HDL) levels in male Wistar strain (Rattus novergicus L) rats fed a high-fat diet. Method: a true experimental research design with a pretest and posttest design with a control group (Pretest and Posttest with Control Group) with simple randomization. Results: There was an increase in HDL levels in the control group (42.50 to 44.50 mg / dl), the olive oil treatment group was (50.83 to 53.33) mg / dL, the moderate intensity exercise treatment group (46.67 to 52 , 00 mg / dl), and the moderate-intensity exercise and olive oil treatment groups (47.50 to 58.67 mg / dl). Conclusion: Extra Virgine Olive Oil, moderate intensity exercise, and Extra Virgine Olive Oil with moderate intensity exercise influence the increase of HDL levels in the blood of male Wistar strain rats fed a high-fatty diet.","PeriodicalId":32215,"journal":{"name":"Journal of Muhammadiyah Medical Laboratory Technologist","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86619900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Based on Indonesia Ministry of Health in 2010, about 75% of woman ever had a vaginal discharge at least once in a lifetime and 45% of woman had vaginal discharge twice or more. The most common risk factor that causing pathological fluor albus is genital organ hygiene. Purposes: The objective of this study is to determine the characteristics of pathological fluor albus on outpatient in RSIA Permata Serdang in 2019. Methods: This was an observational study based on medical record samples in 74 patients with pathological fluor albus between January 2019 and December 2019. Results: From 74 patients with pathological fluor albus, the highest age range occurred 25-29 years age category (24.3%), with the most marital status was married (97.3%), and the most dominant occupation was housewife (51.4%) and mostly living in Serang (52.7%). Clinical manifestations were itching and odor (28.4%), itching (27%), itching with pain and odor (14.9%), odor (10.8%). The secret color is clear (86.5%) followed by white-coloured secret (13.5%). Physical examination have been done with speculum examination (77%). Management provided for the patients are metronidazole (86.5%) and fluconazole (13.5%). Conclusion: Pathological fluor albus at RSIA Permata Serdang occurs often aged 25-29 years in a married woman with the common characteristic complaint was itching with odor.
{"title":"Characteristics of Pathological Fluor Albus on Outpatient in Permata Serdang Mother and Child Hospital Year 2019","authors":"H. E. Monintja, Adinta Anandani","doi":"10.24853/mmj.1.2.15-20","DOIUrl":"https://doi.org/10.24853/mmj.1.2.15-20","url":null,"abstract":"Background: Based on Indonesia Ministry of Health in 2010, about 75% of woman ever had a vaginal discharge at least once in a lifetime and 45% of woman had vaginal discharge twice or more. The most common risk factor that causing pathological fluor albus is genital organ hygiene. Purposes: The objective of this study is to determine the characteristics of pathological fluor albus on outpatient in RSIA Permata Serdang in 2019. Methods: This was an observational study based on medical record samples in 74 patients with pathological fluor albus between January 2019 and December 2019. Results: From 74 patients with pathological fluor albus, the highest age range occurred 25-29 years age category (24.3%), with the most marital status was married (97.3%), and the most dominant occupation was housewife (51.4%) and mostly living in Serang (52.7%). Clinical manifestations were itching and odor (28.4%), itching (27%), itching with pain and odor (14.9%), odor (10.8%). The secret color is clear (86.5%) followed by white-coloured secret (13.5%). Physical examination have been done with speculum examination (77%). Management provided for the patients are metronidazole (86.5%) and fluconazole (13.5%). Conclusion: Pathological fluor albus at RSIA Permata Serdang occurs often aged 25-29 years in a married woman with the common characteristic complaint was itching with odor.","PeriodicalId":32215,"journal":{"name":"Journal of Muhammadiyah Medical Laboratory Technologist","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87416959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of drugs is one of the most common causes of liver injury, because the liver is the main organ that metabolizes drugs. Little is currently done if there is a liver injury due to the hepatotoxic side effects of a drug. Herbal plants have active natural compounds that have pharmacological effects so they are widely used as alternative treatments. Sweet basil (Ocimum basilicum) is one of the most cultivated plants in Asia. Studies on the use of Ocimum basilicum in medicine have been carried out, one of which is the hepatoprotector effect. Studies indicate that Ocimum basilicum is rich in high antioxidant content (flavonoids, saponins, tannins, sterols, triterpenes, and rosmaniric acid) capable of providing hepatoprotector effects by helping the regeneration process of hepatocyte cells that are damaged by hepatotoxic agents and significantly decreasing liver damage biomarkers. The purpose of this review is to explain the potential of Ocimum basilicum as a hepatoprotective agent for liver injury associated with drugs. The conclusion of this review is Ocimum basilicum has high potential in its utilization as a hepatoprotector against liver injury mainly related to the consumption of drugs that have hepatotoxic effects.
{"title":"Potential of Sweet Basil (Ocimum basilicum) as a Hepatoprotector Agent for Liver Injury Related to Drugs","authors":"D. Renovaldi, Abdulgadir K. Adam","doi":"10.24853/mmj.1.2.21-26","DOIUrl":"https://doi.org/10.24853/mmj.1.2.21-26","url":null,"abstract":"The use of drugs is one of the most common causes of liver injury, because the liver is the main organ that metabolizes drugs. Little is currently done if there is a liver injury due to the hepatotoxic side effects of a drug. Herbal plants have active natural compounds that have pharmacological effects so they are widely used as alternative treatments. Sweet basil (Ocimum basilicum) is one of the most cultivated plants in Asia. Studies on the use of Ocimum basilicum in medicine have been carried out, one of which is the hepatoprotector effect. Studies indicate that Ocimum basilicum is rich in high antioxidant content (flavonoids, saponins, tannins, sterols, triterpenes, and rosmaniric acid) capable of providing hepatoprotector effects by helping the regeneration process of hepatocyte cells that are damaged by hepatotoxic agents and significantly decreasing liver damage biomarkers. The purpose of this review is to explain the potential of Ocimum basilicum as a hepatoprotective agent for liver injury associated with drugs. The conclusion of this review is Ocimum basilicum has high potential in its utilization as a hepatoprotector against liver injury mainly related to the consumption of drugs that have hepatotoxic effects.","PeriodicalId":32215,"journal":{"name":"Journal of Muhammadiyah Medical Laboratory Technologist","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85916889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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