The microflora of the intestinal tract is vital to many physiological functions, mainly fermentation and processing of dietary components, control of intestinal epithelial cell proliferation, development of the immune system, and protection against pathogens. Plant extracts have potential for treatment options that protect commensal or beneficial microflora in the intestines while eliminating pathogens. The aim of the present study was to investigate the influence of Pinus brutia (Turkish red pine) bark extract containing phenolic compounds on some commensal and pathogenic bacteria from the intestinal microflora using a microdilution method. P. brutia bark extract did not completely inhibit any intestinal bacteria. However, the extract showed a potential inhibitor activity on Salmonella Typhimurium and Staphylococcus aureus from 75 µg/mL, on Escherichia coli and Fusobacterium nucleatum from 150 µg/mL, and on Clostridium perfringens from 300 µg/mL concentrations (P<0.05). Commensal bacteria were observed to be less sensitive to the extract than those of the pathogenic strains. The extract stimulated moderately the growth of Bifidobacterium bifidum from 75 µg/mL dose (P<0.05). The extract did not show any activity on Lactobacillus acidophilus. A potential inhibitor activity was observed for Bifidobacterium infantis and Lactobacillus casei at 600-2400 µg/mL (P<0.05). As a conclusion, P. brutia bark extract, at 75-300 μg/mL dose range, had a potential to restrict pathogenic bacteria in the intestines while protect commensal or beneficial ones. Specified effects might be mainly attributed to its polyphenolic content.
{"title":"Influence of Pinus brutia bark extract containing phenolic compounds on some commensal and pathogenic bacteria from the intestinal microflora","authors":"A. Demirtas","doi":"10.24880/maeuvfd.709662","DOIUrl":"https://doi.org/10.24880/maeuvfd.709662","url":null,"abstract":"The microflora of the intestinal tract is vital to many physiological functions, mainly fermentation and processing of dietary components, control of intestinal epithelial cell proliferation, development of the immune system, and protection against pathogens. Plant extracts have potential for treatment options that protect commensal or beneficial microflora in the intestines while eliminating pathogens. The aim of the present study was to investigate the influence of Pinus brutia (Turkish red pine) bark extract containing phenolic compounds on some commensal and pathogenic bacteria from the intestinal microflora using a microdilution method. P. brutia bark extract did not completely inhibit any intestinal bacteria. However, the extract showed a potential inhibitor activity on Salmonella Typhimurium and Staphylococcus aureus from 75 µg/mL, on Escherichia coli and Fusobacterium nucleatum from 150 µg/mL, and on Clostridium perfringens from 300 µg/mL concentrations (P<0.05). Commensal bacteria were observed to be less sensitive to the extract than those of the pathogenic strains. The extract stimulated moderately the growth of Bifidobacterium bifidum from 75 µg/mL dose (P<0.05). The extract did not show any activity on Lactobacillus acidophilus. A potential inhibitor activity was observed for Bifidobacterium infantis and Lactobacillus casei at 600-2400 µg/mL (P<0.05). As a conclusion, P. brutia bark extract, at 75-300 μg/mL dose range, had a potential to restrict pathogenic bacteria in the intestines while protect commensal or beneficial ones. Specified effects might be mainly attributed to its polyphenolic content.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Aluminum Obtained from Natural Conditions on In Vitro Spermatological Parameters at Low Doses","authors":"Abdülkadir Kaya, Ö. Varışlı, H. Ekici, S. Kizil","doi":"10.24880/maeuvfd.648962","DOIUrl":"https://doi.org/10.24880/maeuvfd.648962","url":null,"abstract":"","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48441044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soner Tutun, M. Kaya, Melike Sultan Usluer, H. Tutun
St. Johns wort (Hypericum perforatum) is a medicinal plant that exhibits important biological activities exhibit important biological activities being antioxidant, anti-inflammatory, immunomodulatory and/or antitumour. The aim of this study was to investigate antiproliferative effect of H. perforatum oil purchased from a commercial vendor on mouse myeloma cells. The cells were treated with various concentration (10%, 5%, 2%, 1%, 0.5%, 0.25%, 0.2% and 0.1%) of dimethyl sulfoxide (DMSO) to determine the non-toxic concentration. The cells were treated with various concentrations (15.6-500 ppm) of the oil dissolved in DMSO to examine its antiproliferative activity. Non-toxic dose of DMSO was at the concentration of lower than 0.2%. No effect on the cell proliferation was observed in the applied concentration of the oil. In conclusion, the oil had no antiproliferative effect on Myeloma cells at these concentrations.
{"title":"Investigation of antiproliferative effects of Hypericum perforatum oil on myeloma cells","authors":"Soner Tutun, M. Kaya, Melike Sultan Usluer, H. Tutun","doi":"10.24880/maeuvfd.728975","DOIUrl":"https://doi.org/10.24880/maeuvfd.728975","url":null,"abstract":"St. Johns wort (Hypericum perforatum) is a medicinal plant that exhibits important biological activities exhibit important biological activities being antioxidant, anti-inflammatory, immunomodulatory and/or antitumour. The aim of this study was to investigate antiproliferative effect of H. perforatum oil purchased from a commercial vendor on mouse myeloma cells. The cells were treated with various concentration (10%, 5%, 2%, 1%, 0.5%, 0.25%, 0.2% and 0.1%) of dimethyl sulfoxide (DMSO) to determine the non-toxic concentration. The cells were treated with various concentrations (15.6-500 ppm) of the oil dissolved in DMSO to examine its antiproliferative activity. Non-toxic dose of DMSO was at the concentration of lower than 0.2%. No effect on the cell proliferation was observed in the applied concentration of the oil. In conclusion, the oil had no antiproliferative effect on Myeloma cells at these concentrations.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Karakurt, H. Nuhoğlu, S. Dağ, A. G. Sağlam, E. Beytut, M. Sahin, S. Otlu, Ö. Çelebi
Kampilobakteriyozis, sigir ve koyun yetistiriciliginde ekonomik kayiplara yol acan yavru atimi ve infertilite ile karakterize, bulasici ve zoonotik bir infeksiyondur. Bu calismada, Kars yoresinde meydana gelen sigir ve koyun abort vakalarini Kampilobakteriyozis yonunden incelemek ve sonuclari PCR, immunohistokimyasal, histopatolojik ve mikrobiyolojik olarak degerlendirmek amaclanmistir. Bu kapsamda, 2013-2018 yillari arasinda Kafkas Universitesi Veteriner Fakultesi Patoloji Anabilim Dalina Kars merkez ve ilcelerinden getirilen 444 adet atik vakasina ait karaciger ve akciger doku ornekleri ile abomazum icerikleri incelenmistir. Hayvanlardan alinan doku ornekleri % 10’luk tamponlu formaldehit solusyonunda tespit edildi. Rutin islemlerin ardindan hazirlanan parafin bloklardan, Hematoksilen Eozin boyamasi icin 5 μm, immunohistokimyasal boyama icin kalinliginda 4 μm kesitler alindi. Histopatolojik degisikliklerin belirlenmesi amaciyla kesitler isik mikroskobunda incelendi. Abort vakalarina ait organlar ve abomasum icerikleri mikrobiyolojik inceleme amaciyla selektif supplement iceren Preston Campylobacter Enrichment Broth icerisine inokule edilerek on zenginlestirmeye ve daha sonra Preston Campylobacter Selektif Agara gecilerek inkube edildi. Inkubasyon sonrasi uremenin oldugu kulturler koloni morfolojisi ve mikroskobik gorunum yonunden incelendi ve Campylobacter spp. yonunden supheli gorulen koloniler biyokimyasal testlere tabi tutularak degerlendirildi. Yapilan histopatolojik incelemeler sonucunda karakteristik olarak hedef tahtasi gorunumunde multifokal nekrotik hepatitis tablosu gozlenen ve abomazum icerigi sari renkte, bulanik ve pihti iceren 17 adet abort vakasinin 7’si Camphylobacter ssp. yonunden PCR, immunohistokimyasal ve mikrobiyolojik yontemlerle pozitif bulundu. Sonuc olarak Kars yoresinde meydana gelen atik vakalari icerisinde Kampilobakteriyozis’in onemli bir yeri oldugu ve yetistiricilikte dikkate alinmasi gerektigi dusunmekteyiz.
{"title":"Investigation of Campylobacteriosis in Abort Cases in Kars Province by Pathological, Immunohistochemical, PCR and Microbiological Methods","authors":"E. Karakurt, H. Nuhoğlu, S. Dağ, A. G. Sağlam, E. Beytut, M. Sahin, S. Otlu, Ö. Çelebi","doi":"10.24880/maeuvfd.673903","DOIUrl":"https://doi.org/10.24880/maeuvfd.673903","url":null,"abstract":"Kampilobakteriyozis, sigir ve koyun yetistiriciliginde ekonomik kayiplara yol acan yavru atimi ve infertilite ile karakterize, bulasici ve zoonotik bir infeksiyondur. Bu calismada, Kars yoresinde meydana gelen sigir ve koyun abort vakalarini Kampilobakteriyozis yonunden incelemek ve sonuclari PCR, immunohistokimyasal, histopatolojik ve mikrobiyolojik olarak degerlendirmek amaclanmistir. Bu kapsamda, 2013-2018 yillari arasinda Kafkas Universitesi Veteriner Fakultesi Patoloji Anabilim Dalina Kars merkez ve ilcelerinden getirilen 444 adet atik vakasina ait karaciger ve akciger doku ornekleri ile abomazum icerikleri incelenmistir. Hayvanlardan alinan doku ornekleri % 10’luk tamponlu formaldehit solusyonunda tespit edildi. Rutin islemlerin ardindan hazirlanan parafin bloklardan, Hematoksilen Eozin boyamasi icin 5 μm, immunohistokimyasal boyama icin kalinliginda 4 μm kesitler alindi. Histopatolojik degisikliklerin belirlenmesi amaciyla kesitler isik mikroskobunda incelendi. Abort vakalarina ait organlar ve abomasum icerikleri mikrobiyolojik inceleme amaciyla selektif supplement iceren Preston Campylobacter Enrichment Broth icerisine inokule edilerek on zenginlestirmeye ve daha sonra Preston Campylobacter Selektif Agara gecilerek inkube edildi. Inkubasyon sonrasi uremenin oldugu kulturler koloni morfolojisi ve mikroskobik gorunum yonunden incelendi ve Campylobacter spp. yonunden supheli gorulen koloniler biyokimyasal testlere tabi tutularak degerlendirildi. Yapilan histopatolojik incelemeler sonucunda karakteristik olarak hedef tahtasi gorunumunde multifokal nekrotik hepatitis tablosu gozlenen ve abomazum icerigi sari renkte, bulanik ve pihti iceren 17 adet abort vakasinin 7’si Camphylobacter ssp. yonunden PCR, immunohistokimyasal ve mikrobiyolojik yontemlerle pozitif bulundu. Sonuc olarak Kars yoresinde meydana gelen atik vakalari icerisinde Kampilobakteriyozis’in onemli bir yeri oldugu ve yetistiricilikte dikkate alinmasi gerektigi dusunmekteyiz.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gülsüm Öksüztepe, Pelin Demir, Pinar Karatepe, Selçuk Alan, Müzeyyen Akgöl
Bu calisma Elazig’da satilan 25’er adet sade, meyveli ve light ticari kefir orneklerinin bazi kalite parametrelerini belirlemek icin planlandi. Kefir orneklerinin mikrobiyolojik (toplam mezofilik aerob bakteri (TMA), Lactobacillus-Leuconostoc-Pediococcus (LLP), laktik streptokoklar, koliform, proteolitik ve lipolitik bakteri, maya ve kuf, Escherichia coli, Staphylococcus-Micrococcus ve koagulaz pozitif Staphylococcus aureus) ve kimyasal (pH, asitlik, yag, kuru madde, yagsiz kuru madde ve kul) analizleri yapildi. Incelenen tum kefir cesitlerinin hepsinin toplam mezofil aerob (TMA) ve koliform bakteri sayisi bakimindan Turk Gida Kodeksi Fermente Sut Urunleri Tebligi’ne uygun oldugu goruldu. Maya sayisi bakimindan sade 6 (%24), meyveli 5 (%20) ve light kefir orneklerinin ise 1 (%4) tanesinin; kuf sayisi bakimindan sade 3 (%12), meyveli 10 (%40) ve light kefir orneklerinin ise 1 (%4) tanesinin teblige uygunluk gosterdigi saptandi. Ayrica asitlik ve yag miktari bakimindan da incelenen tum kefir orneklerinin teblige uygun oldugu goruldu.
A. Akin, Cevat Sipahi, Mustafa Bahadır Çevrimli, Burak Mat, Aytekin Günlü
Hayvansal uretim faaliyeti gerceklestiren ciftliklerde Insan Kaynaklari Yonetimi (IKY) konusu, isletme yonetici/sahipleri tarafindan genellikle goz ardi edilebilen bir husus olmaktadir. Hâlbuki hayvansal uretimde ekipman sayisi ve teknoloji kullanimi artmasina ragmen, ciftliklerde nitelikli isgucunun onemi azalmamaktadir. Bunun en onemli sebeplerinden birisi hayvansal uretimin temelinde canli suje olmasi ve deneyimli personelin varliginin ciftligin hedefledigi basariya ulasmasini kolaylastirmasidir. Bu arastirma ile Turkiye’de orneklem dahilinde buyukbas hayvancilik isletmelerinde isletme yoneticilerinin/sahiplerinin; isgucu yonetimi, personel temini ve devamliligi konularindaki temel uygulamalari ile bu konudaki gorus ve beklentilerinin ortaya konulmasi amaclanmistir. Bu kapsamda Aksaray, Burdur, Isparta ve Konya illeri genelinde 65 ciftlik yoneticisi/sahibi ile yuz yuze gorusmeler gerceklestirilmistir. Calismada kullanilan “Ciftlik Yoneticisi Is Gucu Memnuniyeti Formu” ciftlik yonetici veteriner hekim, isletme mudurleri, akademisyenler gibi alaninda uzman kisilerle birlikte Delphi sureci ile gelistirilmistir. Ciftlik Yoneticisi Is Gucu Memnuniyeti-Olcegi sorulari sirasi ile “Personelle Iletisim”, “Ciftlik Basarisinda Personel”, “Terfi ve Aidiyet” ve “Isgucu Yonetimi” olmak uzere 4 faktor altinda isimlendirilmistir. Elde edilen veriler faktor analizi yontemi ile degerlendirilmistir. Ilgili olcegin verilerinin faktor analizine uygun oldugu (p<0,05) ve genel olarak faktor analizi sonuclarina gore olcme aracinin yapi gecerliliginin saglandigi soylenebilir. Sonuc itibariyle Turkiye’de hayvancilik sektorunde insan kaynaklari yonetimi, artan olcek ve yatirim miktarlari ile her gecen gun daha da onem kazanmaktadir. Orta ve buyuk olcekli isletmelerde kalifiye personelin devamliligi, personel verimliligi isletmelerin karliligini ve ekonomik hedeflerine ulasabilmesinde dogrudan rol oynamaktadir. Bu nedenle hayvancilik sektorunde yonetici pozisyonundaki kisilerin degisen uretim yapisi ile birlikte ciftliklerinde isgucu memnuniyetini artirmalari, Insan Kaynaklari Yonetimi (IKY) konusunda gerekli egitimi almalari ve sahada-isletmelerinde buna yonelik uygulamalari hayata gecirmeleri ciftligin ekonomik hedeflerine basariyla ulasmasinda hayati bir oneme sahiptir.
在发生动物尿毒症活动的农场，通常是农药的主题，然后是人力资源管理（IKY）。然而，ragmen在动物利尿剂方面使用的设备和技术数量增加，这降低了肥料的质量。最重要的原因之一是在动物尿素的基础上促进生活用水，并促进有经验的人员获得目标。土耳其的一个城市是一个城市，它是一个年轻的城市；目的是通过工作人员的基本应用和连续性培养这方面的好奇心和期望。在本章中，据报道，Aksaray、Burdur、Isparta和Conya经常被65种化肥/盐的所有者污染。Calisma中使用的“文明Yonetics Is Gucu Pleasure Form”是与科学、智慧和学术领域的专家共同开发的，具有Delphi的形象。生育的概念是Gucu Pleasure Olcegi问题在以下4个因素下被提及：Sirsi和“个人Iletisi”、“Ciftlik Basarisinda Personel”、“Terfi和Aidiyet”和“Isgucu Yonetimi”。使用因子分析单元对收集到的数据进行降级。根据初级嗅觉数据的因子分析（p＜0.05），因子分析通常表明车辆的结构长度在更大程度上缩短。因此，在土耳其的动物行业，人力资源将增加，教育量将增加，每晚收入将超过1%。工作人员素质的连续性、人员和经济目标的复杂性，是为了在实现人员需求的复杂性方面发挥真正的作用。因此，至关重要的是，动物部门中那些不处于离子位置的人，以及尿道植物，将享受他们的农场，采取必要的Insan资源预防（IKY）结构，并通过肥料的经济目标，使他们的应用恢复生机。
{"title":"Büyükbaş Hayvancılık İşletmelerinde Yöneticilerin İşgücü Memnuniyet Düzeyleri","authors":"A. Akin, Cevat Sipahi, Mustafa Bahadır Çevrimli, Burak Mat, Aytekin Günlü","doi":"10.24880/maeuvfd.740422","DOIUrl":"https://doi.org/10.24880/maeuvfd.740422","url":null,"abstract":"Hayvansal uretim faaliyeti gerceklestiren ciftliklerde Insan Kaynaklari Yonetimi (IKY) konusu, isletme yonetici/sahipleri tarafindan genellikle goz ardi edilebilen bir husus olmaktadir. Hâlbuki hayvansal uretimde ekipman sayisi ve teknoloji kullanimi artmasina ragmen, ciftliklerde nitelikli isgucunun onemi azalmamaktadir. Bunun en onemli sebeplerinden birisi hayvansal uretimin temelinde canli suje olmasi ve deneyimli personelin varliginin ciftligin hedefledigi basariya ulasmasini kolaylastirmasidir. Bu arastirma ile Turkiye’de orneklem dahilinde buyukbas hayvancilik isletmelerinde isletme yoneticilerinin/sahiplerinin; isgucu yonetimi, personel temini ve devamliligi konularindaki temel uygulamalari ile bu konudaki gorus ve beklentilerinin ortaya konulmasi amaclanmistir. Bu kapsamda Aksaray, Burdur, Isparta ve Konya illeri genelinde 65 ciftlik yoneticisi/sahibi ile yuz yuze gorusmeler gerceklestirilmistir. Calismada kullanilan “Ciftlik Yoneticisi Is Gucu Memnuniyeti Formu” ciftlik yonetici veteriner hekim, isletme mudurleri, akademisyenler gibi alaninda uzman kisilerle birlikte Delphi sureci ile gelistirilmistir. Ciftlik Yoneticisi Is Gucu Memnuniyeti-Olcegi sorulari sirasi ile “Personelle Iletisim”, “Ciftlik Basarisinda Personel”, “Terfi ve Aidiyet” ve “Isgucu Yonetimi” olmak uzere 4 faktor altinda isimlendirilmistir. Elde edilen veriler faktor analizi yontemi ile degerlendirilmistir. Ilgili olcegin verilerinin faktor analizine uygun oldugu (p<0,05) ve genel olarak faktor analizi sonuclarina gore olcme aracinin yapi gecerliliginin saglandigi soylenebilir. Sonuc itibariyle Turkiye’de hayvancilik sektorunde insan kaynaklari yonetimi, artan olcek ve yatirim miktarlari ile her gecen gun daha da onem kazanmaktadir. Orta ve buyuk olcekli isletmelerde kalifiye personelin devamliligi, personel verimliligi isletmelerin karliligini ve ekonomik hedeflerine ulasabilmesinde dogrudan rol oynamaktadir. Bu nedenle hayvancilik sektorunde yonetici pozisyonundaki kisilerin degisen uretim yapisi ile birlikte ciftliklerinde isgucu memnuniyetini artirmalari, Insan Kaynaklari Yonetimi (IKY) konusunda gerekli egitimi almalari ve sahada-isletmelerinde buna yonelik uygulamalari hayata gecirmeleri ciftligin ekonomik hedeflerine basariyla ulasmasinda hayati bir oneme sahiptir.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, it is aimed to determine the microbiological quality of 60 different raw salmon finger sushi (maki rolls) samples randomly supplied from consumption points (sushi bar, sushi buffet, hotel, restaurant etc.) serving fresh sushi. The counts of Escherichia coli, coagulase positive Staphylococcus aureus, Vibrio paraheamolyticus and Salmonella spp. presence (+/-) were examined in these samples for the detection of hygienic quality and pathogenic microorganisms. Twenty-nine of 29/60 (48.3%) samples were found to be positive for these microorganisms. Salmonella spp. presence was detected in 2/60 samples (3.3%) while coagulase positive S. aureus was found in 10/60 samples (16.7%) with a highest level of 4.84 log CFU/g. E. coli was detected in 15/60 samples (25%), V. paraheamolyticus was determined in 13/60 samples (21.7%) with a level of 2.90 log CFU/g and 2.82 log CFU/g, respectively. Based on the current findings, it is considered that microorganisms determined in the samples pose a risk to public health, authorities should mandate to conduct routine inspections more frequently and businesses selling sushi should be included in the annual sampling plan. It will be beneficial for healthy sushi production that it should be paid attention to hygienic production during the all manufacturing, storage and service stages in the sushi production and points of sale and relevant personnel should be ensured to routinely receive necessary food safety training to minimize the risk of foodborne disease outbreaks.
{"title":"Evaluation of Microbiological Quality in Fresh Sushi Samples","authors":"H. Yalçin, T. Çakmak","doi":"10.24880/maeuvfd.680767","DOIUrl":"https://doi.org/10.24880/maeuvfd.680767","url":null,"abstract":"In this study, it is aimed to determine the microbiological quality of 60 different raw salmon finger sushi (maki rolls) samples randomly supplied from consumption points (sushi bar, sushi buffet, hotel, restaurant etc.) serving fresh sushi. The counts of Escherichia coli, coagulase positive Staphylococcus aureus, Vibrio paraheamolyticus and Salmonella spp. presence (+/-) were examined in these samples for the detection of hygienic quality and pathogenic microorganisms. Twenty-nine of 29/60 (48.3%) samples were found to be positive for these microorganisms. Salmonella spp. presence was detected in 2/60 samples (3.3%) while coagulase positive S. aureus was found in 10/60 samples (16.7%) with a highest level of 4.84 log CFU/g. E. coli was detected in 15/60 samples (25%), V. paraheamolyticus was determined in 13/60 samples (21.7%) with a level of 2.90 log CFU/g and 2.82 log CFU/g, respectively. Based on the current findings, it is considered that microorganisms determined in the samples pose a risk to public health, authorities should mandate to conduct routine inspections more frequently and businesses selling sushi should be included in the annual sampling plan. It will be beneficial for healthy sushi production that it should be paid attention to hygienic production during the all manufacturing, storage and service stages in the sushi production and points of sale and relevant personnel should be ensured to routinely receive necessary food safety training to minimize the risk of foodborne disease outbreaks.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Derived from mesenchymal stem cells (MSCs) under the influence of various local factors and growth hormones, osteoblasts are one of the leading components of the bone tissue. 1,25-dihydroxyvitamin D3 (calcitriol), an active form of vitamin D, plays an important role in retaining calcium and phosphate homeostasis within the body. Since vitamin D is also known to have effects on cell proliferation, our study aimed to investigate the effects of different concentrations of calcitriol on the proliferation of in vitro cultured osteoblast-like cells from the bone marrow of 90-95 days old young adult rats of both sexes. Calcitriol was applied at six (6) different concentrations (nM): 0.1, 1, 10, 20, 40 i 60. Each concentration was tested four times, counting the cells after 24 and 48 hours. After 24 and 48 hours treatment, the most effective dose of calcitriol for cell proliferation was 0.1 nM for both males and females. The greatest reduction in the frequency of OB-like cells from both sexes after treatment of 24 and 48 hours, was a 60 nM calcitriol concentration. Higher proliferative values of osteoblast-like cells were obtained in males compared to those obtained in females. Thus, the results of the current study reveal that calcitriol treatment induces the proliferation of osteoblast-like cells in a dose-dependent manner and this effect shows difference in cells from different genders.
{"title":"The effect of Calcitriol 1,25 (OH)2 - D3 on osteoblast-like cell proliferation during in vitro cultivation","authors":"M. Kati̇ca, Filiz Tepekoy","doi":"10.24880/maeuvfd.653000","DOIUrl":"https://doi.org/10.24880/maeuvfd.653000","url":null,"abstract":"Derived from mesenchymal stem cells (MSCs) under the influence of various local factors and growth hormones, osteoblasts are one of the leading components of the bone tissue. 1,25-dihydroxyvitamin D3 (calcitriol), an active form of vitamin D, plays an important role in retaining calcium and phosphate homeostasis within the body. Since vitamin D is also known to have effects on cell proliferation, our study aimed to investigate the effects of different concentrations of calcitriol on the proliferation of in vitro cultured osteoblast-like cells from the bone marrow of 90-95 days old young adult rats of both sexes. Calcitriol was applied at six (6) different concentrations (nM): 0.1, 1, 10, 20, 40 i 60. Each concentration was tested four times, counting the cells after 24 and 48 hours. After 24 and 48 hours treatment, the most effective dose of calcitriol for cell proliferation was 0.1 nM for both males and females. The greatest reduction in the frequency of OB-like cells from both sexes after treatment of 24 and 48 hours, was a 60 nM calcitriol concentration. Higher proliferative values of osteoblast-like cells were obtained in males compared to those obtained in females. Thus, the results of the current study reveal that calcitriol treatment induces the proliferation of osteoblast-like cells in a dose-dependent manner and this effect shows difference in cells from different genders.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu calismanin amaci, multidedektor bilgisayarli tomografi (MDBT) goruntuleri kullanarak uc boyutlu bilgisayar programi araciligi ile elde edilen uc boyutlu (3B) rekonstruksiyonlara dayanarak tilki cervical vertebrae’nin morfometrik ozelliklerinin belirlenmesidir. Farkli zamanlarda trafik kazasinda elde edilen toplam 6 erkek tilki ( Vulpes vulpes ) kullanildi. Yuzustu pozisyonda genel bir diagnostik MDBT kullanilarak cervical vertebrae’nin yuksek cozunurluklu goruntuleri elde edildi ve goruntuler 3B bir modelleme programi (Mimics) olan bir bilgisayara aktarildi. Her bir vertebrae’nin (atlas haric) corpus vertebrae uzunlugu, corpus vertebrae’nin transvers ve sagital capi, foramen vertebrae’nin transvers ve sagittal caplari, olusturulan 3B modelden olculdu ve indeksler hesaplandi. Her bir olcum degerlerinin ortalamalari hesaplandi. Corpus vertebrae uzunlugunun C2'den C7'ye azaldigi, transvers capinin C2'den C4'e azaldigi, ancak C5'ten C7'ye arttigi, sagittal capi transvers capin aksine C2'den C4'e artarken, C5'ten C7'ye azaldigi, foramen vertebrae’nin transvers ve sagital caplari C5'ten C7'ye arttigi gorulmustur. Bu calisma tilki cervical vertebrae uzerinde yapilan ilk 3B rekonstruksiyondur ve sonuclar bu turun anatomi bilgisine katkida bulunabilir. Ayrica bu teknik, diger vahsi hayvanlara zarar vermeden anatomilerini anlamak icin de kullanilabilir.
{"title":"Tilki (Vulpes vulpes) Cervical Vertebrae'nın Morfometrik Özelliklerinin Üç Boyutlu Rekonstrüksiyon Kullanarak İncelenmesi","authors":"Sema Özkadif, A. Haligür","doi":"10.24880/maeuvfd.629362","DOIUrl":"https://doi.org/10.24880/maeuvfd.629362","url":null,"abstract":"Bu calismanin amaci, multidedektor bilgisayarli tomografi (MDBT) goruntuleri kullanarak uc boyutlu bilgisayar programi araciligi ile elde edilen uc boyutlu (3B) rekonstruksiyonlara dayanarak tilki cervical vertebrae’nin morfometrik ozelliklerinin belirlenmesidir. Farkli zamanlarda trafik kazasinda elde edilen toplam 6 erkek tilki ( Vulpes vulpes ) kullanildi. Yuzustu pozisyonda genel bir diagnostik MDBT kullanilarak cervical vertebrae’nin yuksek cozunurluklu goruntuleri elde edildi ve goruntuler 3B bir modelleme programi (Mimics) olan bir bilgisayara aktarildi. Her bir vertebrae’nin (atlas haric) corpus vertebrae uzunlugu, corpus vertebrae’nin transvers ve sagital capi, foramen vertebrae’nin transvers ve sagittal caplari, olusturulan 3B modelden olculdu ve indeksler hesaplandi. Her bir olcum degerlerinin ortalamalari hesaplandi. Corpus vertebrae uzunlugunun C2'den C7'ye azaldigi, transvers capinin C2'den C4'e azaldigi, ancak C5'ten C7'ye arttigi, sagittal capi transvers capin aksine C2'den C4'e artarken, C5'ten C7'ye azaldigi, foramen vertebrae’nin transvers ve sagital caplari C5'ten C7'ye arttigi gorulmustur. Bu calisma tilki cervical vertebrae uzerinde yapilan ilk 3B rekonstruksiyondur ve sonuclar bu turun anatomi bilgisine katkida bulunabilir. Ayrica bu teknik, diger vahsi hayvanlara zarar vermeden anatomilerini anlamak icin de kullanilabilir.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Can the pH of the skin be an important biophysical marker in dogs with generalized demodicosis?","authors":"Gizem Gül, K. Ural, S. Erdoğan","doi":"10.24880/maeuvfd.516280","DOIUrl":"https://doi.org/10.24880/maeuvfd.516280","url":null,"abstract":"","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69070131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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