Pub Date : 2022-08-31DOI: 10.24880/maeuvfd.1073019
Gürsel Özmen, M. Kale
Burdur-Merkez ve ilçelerinde halk elinde, farklı yapıdaki işletmelerde bulunan 106 adet dişi ineğin bovine papillomavirus (BPV) kaynaklı gelişen meme siğillerinden doku örnekleri toplandı. BPV tip spesifik primerleri açısından PCR testi ile 106 örneğin hepsinde BPV pozitiflik belirlendi. Çalışmada; işletme tipleri, bireysel hayvan bakım ve besleme koşulları, sağım koşulları ve ahır temizliği parametreler yönünden incelenerek değerlendirildi. Ayrıca, meme siğillerinin makroskobik görünümlerine göre (düz ve yuvarlak, pirinç tanesi, saplı-sivri) sınıflandırılmış, BPV tip spesifik primerleri açısından pozitiflik tespit edilmiş 30 örnekte iki farklı tedavi uygulama yöntemleri çalışıldı. Birinci grupta tasnif edilmiş 15 hayvana otojen aşı, ikinci grupta tasnif edilmiş 15 hayvana Newcastle disease virus (NDV) La Sota suş aşı uygulamaları yapıldı. Otojen aşı uygulamalarının yapıldığı, farklı makroskobik görünümlere sahip meme siğillerinde %100 gerileme ve iyileşme sağlandı. Newcastle disease virus La Sota aşı uygulamalarının yapıldığı farklı makroskobik görünümlere sahip meme siğillerinde %53,3 gerileme ve iyileşme sağlandı. İneklerde meme siğillerinin tedavisinde otojen aşı uygulamalarının daha etkin sonuçlar verdiği tespit edildi. Ayrıca, çalışmada meme siğillerinin oluşumunda; işletme, bakım, besleme, sağım, ahır temizliği ve diğer faktörlerin de etkili olabileceği yorumlandı.
在Burdur Central及其城镇，在公共场所收集了106份在不同建筑工程中发现的来自牛的面粉流行性感冒病毒（BPV）样本。BPV已经确定了所有PCR测试和106个实例的BPV阳性值。在工作中；通过检查评估操作类型、个体动物监测和营养条件、正确条件和倒钩清洁参数。此外，根据乳房注射器（直注射器和圆形注射器、菠萝注射器、菠萝针筒）的宏观外观，已经对30个阳性BPV具体实例应用了两种不同的治疗方法。第一组由15只动物自体组成，在第二组中鉴定，其中15只动物在新城疫病毒（NDV）和La Sota sushi aşe应用中鉴定。通过汽车移植应用，在具有不同宏观视觉效果的乳房注射器中实现100%的减少和恢复。新城疫病毒La Sota aşıvigulamalarının yapıldığıfarklımakroskobik görünümlere sahip meme siğillerinde%53,3 gerileme ve iyileşme sağlandı。在奶牛身上，已经检测到过量使用autogen对治疗乳腺弹珠有更大的影响。此外，当涉及到乳房注射器的创建时，人们注意到，商业、护理、食品、权利、钢铁清洁和其他因素都是有效的。
{"title":"Farklı işletme koşullarında ineklerin meme başlarında bovine papillomavirus kaynaklı gelişen siğillerin tedavisinde uygulanan yöntemlerin (otolog aşı ve newcastle disease virus La sota suşu) değerlendirilmesi","authors":"Gürsel Özmen, M. Kale","doi":"10.24880/maeuvfd.1073019","DOIUrl":"https://doi.org/10.24880/maeuvfd.1073019","url":null,"abstract":"Burdur-Merkez ve ilçelerinde halk elinde, farklı yapıdaki işletmelerde bulunan 106 adet dişi ineğin bovine papillomavirus (BPV) kaynaklı gelişen meme siğillerinden doku örnekleri toplandı. BPV tip spesifik primerleri açısından PCR testi ile 106 örneğin hepsinde BPV pozitiflik belirlendi. Çalışmada; işletme tipleri, bireysel hayvan bakım ve besleme koşulları, sağım koşulları ve ahır temizliği parametreler yönünden incelenerek değerlendirildi. Ayrıca, meme siğillerinin makroskobik görünümlerine göre (düz ve yuvarlak, pirinç tanesi, saplı-sivri) sınıflandırılmış, BPV tip spesifik primerleri açısından pozitiflik tespit edilmiş 30 örnekte iki farklı tedavi uygulama yöntemleri çalışıldı. Birinci grupta tasnif edilmiş 15 hayvana otojen aşı, ikinci grupta tasnif edilmiş 15 hayvana Newcastle disease virus (NDV) La Sota suş aşı uygulamaları yapıldı. Otojen aşı uygulamalarının yapıldığı, farklı makroskobik görünümlere sahip meme siğillerinde %100 gerileme ve iyileşme sağlandı. Newcastle disease virus La Sota aşı uygulamalarının yapıldığı farklı makroskobik görünümlere sahip meme siğillerinde %53,3 gerileme ve iyileşme sağlandı. İneklerde meme siğillerinin tedavisinde otojen aşı uygulamalarının daha etkin sonuçlar verdiği tespit edildi. Ayrıca, çalışmada meme siğillerinin oluşumunda; işletme, bakım, besleme, sağım, ahır temizliği ve diğer faktörlerin de etkili olabileceği yorumlandı.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69068080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.24880/maeuvfd.1133933
A. Özgür
The history of the Agricultural and Civil Veterinary School in Halkalı, where Mehmet Âkif was a student first and then served as a lecturer, is in a very important place in terms of teaching veterinary medicine and agriculture in Turkey. The opening of the Halkalı Agricultural School and the opening of the Civil Veterinary School coincided with the same time period. The Civil Veterinary College was the partner of the Agricultural College opened in Halkalı in the first years. The Civilian Veterinary College was later moved to Sultanahmet, and agricultural education was continued in Halkalı. The teaching staff of the Halkalı Higher Agricultural College has published a journal called “Halkalı Ziraat Mekteb-i Âlisi Mecmuası”. Mehmet Âkif Bey (Ersoy), who was on the teaching staff of the College at that time, wrote an article containing the history of the School in the first issue of the Magazine. The article provides information about the Halkalı Agricultural College, as well as information about the beginning of the teaching of Civil Veterinary College. In the article, besides the information about Halkalı Higher Agricultural School, information about the beginning of civilian veterinary medicine education is also given. In this study, the relationship of Mehmet Âkif Ersoy's article, which was determined to be related to the history of the profession, with the research conducted on the teaching of veterinary medicine in Turkey and the history of agricultural teaching, was analyzed. The article, which contains information about Âkif's profession, was evaluated in terms of the history of veterinary medicine and presented to the field of science history and research ethics.
{"title":"A first look at the history of Turkish veterinary medicine with Mehmet Âkif (Ersoy)","authors":"A. Özgür","doi":"10.24880/maeuvfd.1133933","DOIUrl":"https://doi.org/10.24880/maeuvfd.1133933","url":null,"abstract":"The history of the Agricultural and Civil Veterinary School in Halkalı, where Mehmet Âkif was a student first and then served as a lecturer, is in a very important place in terms of teaching veterinary medicine and agriculture in Turkey. The opening of the Halkalı Agricultural School and the opening of the Civil Veterinary School coincided with the same time period. The Civil Veterinary College was the partner of the Agricultural College opened in Halkalı in the first years. The Civilian Veterinary College was later moved to Sultanahmet, and agricultural education was continued in Halkalı. The teaching staff of the Halkalı Higher Agricultural College has published a journal called “Halkalı Ziraat Mekteb-i Âlisi Mecmuası”. Mehmet Âkif Bey (Ersoy), who was on the teaching staff of the College at that time, wrote an article containing the history of the School in the first issue of the Magazine. The article provides information about the Halkalı Agricultural College, as well as information about the beginning of the teaching of Civil Veterinary College. In the article, besides the information about Halkalı Higher Agricultural School, information about the beginning of civilian veterinary medicine education is also given. In this study, the relationship of Mehmet Âkif Ersoy's article, which was determined to be related to the history of the profession, with the research conducted on the teaching of veterinary medicine in Turkey and the history of agricultural teaching, was analyzed. The article, which contains information about Âkif's profession, was evaluated in terms of the history of veterinary medicine and presented to the field of science history and research ethics.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69069146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.24880/maeuvfd.1076011
S. S. Sabanci
Morphological characteristics of the proximal tibia affect the orthopedic balance of the knee region. Such as the width of the condyles, tibial plateau angle, position of the patella; Intercondylar width is one of these features as well. The objectives of this study are to calculate the intercondylar width index of the tibia (ICWI) in dogs, to determine if there are gender-related differences in ICWI, and to compare the ICWI among the six breeds of dog. For these aims, the tibial bones of 84 dogs from 26 different breeds were used. The radiographs of tibias were taken, and the tibial plateau and intercondylar widths were measured. The intercondylar width index was calculated using the following formula: (intercondylar width/tibial plateau width) *100. The mean intercondylar width index of the dogs was 19.2±2.72. The index values of male and female dogs were 19.1±3.32 and 19.3±2.02, respectively. The significant differences in the index were not determined between male and female dogs, but the significant difference was determined among dogs’ breeds. The dogs in the high-risk breeds had significantly smaller eminence width index value than the dogs in the low-risk breeds related with the non-traumatic cranial cruciate ligament rupture. The results of this study suggested performing further clinical studies to evaluate whether the difference in intercondylar width index among breeds in dogs is associated with cranial cruciate ligament deficiency.
{"title":"Intercondylar width index of the tibia in the dogs: A morphological study","authors":"S. S. Sabanci","doi":"10.24880/maeuvfd.1076011","DOIUrl":"https://doi.org/10.24880/maeuvfd.1076011","url":null,"abstract":"Morphological characteristics of the proximal tibia affect the orthopedic balance of the knee region. Such as the width of the condyles, tibial plateau angle, position of the patella; Intercondylar width is one of these features as well. The objectives of this study are to calculate the intercondylar width index of the tibia (ICWI) in dogs, to determine if there are gender-related differences in ICWI, and to compare the ICWI among the six breeds of dog. For these aims, the tibial bones of 84 dogs from 26 different breeds were used. The radiographs of tibias were taken, and the tibial plateau and intercondylar widths were measured. The intercondylar width index was calculated using the following formula: (intercondylar width/tibial plateau width) *100. The mean intercondylar width index of the dogs was 19.2±2.72. The index values of male and female dogs were 19.1±3.32 and 19.3±2.02, respectively. The significant differences in the index were not determined between male and female dogs, but the significant difference was determined among dogs’ breeds. The dogs in the high-risk breeds had significantly smaller eminence width index value than the dogs in the low-risk breeds related with the non-traumatic cranial cruciate ligament rupture. The results of this study suggested performing further clinical studies to evaluate whether the difference in intercondylar width index among breeds in dogs is associated with cranial cruciate ligament deficiency.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69068225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.24880/maeuvfd.1073085
Muharrem Satar, M. S. Arıkan, Arzu Peker
Bu çalışma ile Türkiye’de 4 ilde pilot olarak uygulanan uzman eller projesi kapsamında büyükbaş ve küçükbaş hayvan yetiştiriciliği projesi uygulayan işletmelerinin sosyo-ekonomik yapısı, proje uygulamasına bakış açıları ve projelerin sürdürebilirliklerine ilişkin tespitlerin ortaya konulması amacıyla yapılmıştır. Uzman eller projesi kapsamında hayvancılık projesinden 50 girişimci faydalandı. Araştırma kapsamında Türkiye genelinde 18 küçükbaş ve 18 büyükbaş toplamda 36 proje girişimcisinden Google anket aracı kullanılarak veriler toplanmıştır. Girişimcilerin %47,2'si daha önce başka bir işte çalıştığı tespit edilmiştir. Girişimcilerin %61,1'i hayvancılık faaliyetine devam edeceğini bildirmiştir. İşletme sahiplerinin %24 finansman ihtiyacını kredi temini ile karşılamaktadır. Girişimcilerin 24'ünün hayvancılık dışında başka bir geliri olmadığı tespit edilmiştir. Kapasite kullanım oranı ortalama %58,6 olarak hesaplanmıştır. Girişimcilerin % 61,1’inin prejeye devam etmek istemesi, gençlerin sektöre olan ilgisinin hala yüksek olduğunu göstermektedir. Proje kapsamında alanında yeterli eğitimi almış olmasına karşın kırsal alanda kendi işini kurmak isteyenler açısından bir fırsat olduğu düşünülmektedir. Projenin bütün illerde uygulanması sektörde eğitim almış girişimcilerle kırsal alana yeni bir dinamizm kazandıracak ve hayvansal üretimin sürdürülebilirliğine katkı sağlayacaktır.
在土耳其，为期四年的专家或专家试点项目旨在揭示实施大型和小型动物生长项目的企业的社会经济结构、项目应用前景和项目的可持续性。一个专家手工项目受益于动物项目中的50项举措。该研究包括谷歌调查工具从土耳其共18名未成年人和18个主要项目发射器收集的数据。Girişimcilerin%47,2'si dahaönce başka bir işteçalıştığıtespit edilmiştir。注意到61.1%的参与者将继续进行动物活动。工人们需要24%的资金来偿还贷款。Girişimcilerin 24'ünün hayvancılık dışında başka bir geliri olmadığıtespit edilmiştir。容量使用率估计为58.6%。61.1%的参与者的目标是继续捕食，这表明年轻人对该行业的兴趣仍然很高。与在项目领域接受足够的培训相比，人们认为，对于那些想做好自己工作的人来说，这是一个机会。该项目将为在该行业接受多年培训的企业家创造新的活力，并为动物生产的可持续性做出贡献。
{"title":"Kırsalda girişimcilik örneği: uzman eller projesi ile desteklenen hayvancılık işletmelerinin sosyo-ekonomik analizi","authors":"Muharrem Satar, M. S. Arıkan, Arzu Peker","doi":"10.24880/maeuvfd.1073085","DOIUrl":"https://doi.org/10.24880/maeuvfd.1073085","url":null,"abstract":"Bu çalışma ile Türkiye’de 4 ilde pilot olarak uygulanan uzman eller projesi kapsamında büyükbaş ve küçükbaş hayvan yetiştiriciliği projesi uygulayan işletmelerinin sosyo-ekonomik yapısı, proje uygulamasına bakış açıları ve projelerin sürdürebilirliklerine ilişkin tespitlerin ortaya konulması amacıyla yapılmıştır. Uzman eller projesi kapsamında hayvancılık projesinden 50 girişimci faydalandı. Araştırma kapsamında Türkiye genelinde 18 küçükbaş ve 18 büyükbaş toplamda 36 proje girişimcisinden Google anket aracı kullanılarak veriler toplanmıştır. Girişimcilerin %47,2'si daha önce başka bir işte çalıştığı tespit edilmiştir. Girişimcilerin %61,1'i hayvancılık faaliyetine devam edeceğini bildirmiştir. İşletme sahiplerinin %24 finansman ihtiyacını kredi temini ile karşılamaktadır. Girişimcilerin 24'ünün hayvancılık dışında başka bir geliri olmadığı tespit edilmiştir. Kapasite kullanım oranı ortalama %58,6 olarak hesaplanmıştır. Girişimcilerin % 61,1’inin prejeye devam etmek istemesi, gençlerin sektöre olan ilgisinin hala yüksek olduğunu göstermektedir. Proje kapsamında alanında yeterli eğitimi almış olmasına karşın kırsal alanda kendi işini kurmak isteyenler açısından bir fırsat olduğu düşünülmektedir. Projenin bütün illerde uygulanması sektörde eğitim almış girişimcilerle kırsal alana yeni bir dinamizm kazandıracak ve hayvansal üretimin sürdürülebilirliğine katkı sağlayacaktır.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69068137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-26DOI: 10.24880/maeuvfd.1120634
R. Kiyici, N. Mamak, S. Sahinduran, Suleyman Sensoy, H. A. Akkan, M. Karaca, R. Yildiz, Yavuz Musabeşeoğlu, Halil İbrahim Gökçe
This work was conducted to determine the prevalence of E. coli, cryptosporidium spp., giardia, rotavirus and coronavirus agents involved in the etiology of diarrhea in cases of neonatal calf diarrhea detected in Burdur Mehmet Akif Ersoy University Animal Hospital Clinics and other farms in the region between 2019-2020 years. The material of the study consisted of 50 neonatal calves with diarrhea. Stool samples were taken from calves with diarrhea and examined with immunochromatographic rapid test kits. One or more enteropathogenic agents were detected in 70% of the calves. In calves with diarrhea, Cryptosporidium spp (42%) and rotavirus (30%) were detected as single and mixed enteropathogen. Mixed enteropathogens (14% Cryptosporidium spp. + Rotavirus, 2% Rotavirus + coronavirus, 2% Cryptosporidium spp. + rotavirus + coronavirus + E. coli K99 + Giardia, 2% Cryptosporidium spp. + coronavirus + E. coli K99 + Giardia) were determined in 20% of calves. As a result, it was concluded that in neonatal calf diarrhea, cryptosporidium and rotavirus factors are common and necessary precautions should be taken in enterprises against these factors.
开展这项工作是为了确定2019-2020年间在Burdur Mehmet Akif Ersoy大学动物医院诊所和该地区其他农场检测到的新生儿小牛腹泻病例中大肠杆菌、隐孢子虫、贾第鞭毛虫、轮状病毒和冠状病毒病原体的腹泻病因。该研究的材料包括50只患有腹泻的新生小牛。从腹泻小牛身上采集粪便样本，并用免疫层析快速检测试剂盒进行检测。在70%的犊牛中检测到一种或多种肠致病性病原体。在犊牛腹泻中，隐孢子虫(42%)和轮状病毒(30%)分别为单一和混合致病菌。20%犊牛中检出混合肠道病原体(隐孢子虫+轮状病毒14%，轮状病毒+冠状病毒2%，隐孢子虫+轮状病毒+大肠杆菌K99 +贾第鞭毛虫2%，隐孢子虫+冠状病毒+大肠杆菌K99 +贾第鞭毛虫2%)。由此得出，在新生儿犊牛腹泻中，隐孢子虫和轮状病毒因子是常见的，企业应采取必要的预防措施。
{"title":"Etiological Examination of Neonatal Calf Diarrhea Cases Detected in Burdur Region","authors":"R. Kiyici, N. Mamak, S. Sahinduran, Suleyman Sensoy, H. A. Akkan, M. Karaca, R. Yildiz, Yavuz Musabeşeoğlu, Halil İbrahim Gökçe","doi":"10.24880/maeuvfd.1120634","DOIUrl":"https://doi.org/10.24880/maeuvfd.1120634","url":null,"abstract":"This work was conducted to determine the prevalence of E. coli, cryptosporidium spp., giardia, rotavirus and coronavirus agents involved in the etiology of diarrhea in cases of neonatal calf diarrhea detected in Burdur Mehmet Akif Ersoy University Animal Hospital Clinics and other farms in the region between 2019-2020 years. The material of the study consisted of 50 neonatal calves with diarrhea. Stool samples were taken from calves with diarrhea and examined with immunochromatographic rapid test kits. One or more enteropathogenic agents were detected in 70% of the calves. In calves with diarrhea, Cryptosporidium spp (42%) and rotavirus (30%) were detected as single and mixed enteropathogen. Mixed enteropathogens (14% Cryptosporidium spp. + Rotavirus, 2% Rotavirus + coronavirus, 2% Cryptosporidium spp. + rotavirus + coronavirus + E. coli K99 + Giardia, 2% Cryptosporidium spp. + coronavirus + E. coli K99 + Giardia) were determined in 20% of calves. As a result, it was concluded that in neonatal calf diarrhea, cryptosporidium and rotavirus factors are common and necessary precautions should be taken in enterprises against these factors.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77289289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-09DOI: 10.24880/maeuvfd.1126466
Ayşe Kizilyer
Multiple myeloma cell lines are difficult to transfect with non-viral nucleic acid delivery methods. While electroporation is the most efficient tool for the transfection of most hard-to-transfect cells, human multiple myeloma cells differ in their permissiveness and each cell type require different electroporation conditions for an efficient transgene delivery. In this study, various parameters for NCI H929 human multiple myeloma cells are tested to generate an optimized electroporation protocol. Findings from this paper showed that besides the voltage and capacitance settings, cell count, the cell cycle status of cells, the amount of nucleic acid and removal of death cells all impacted the electroporation efficiency and viable cell count. These results are expected to serve as a starting point and a guide for researchers.
{"title":"An optimized protocol for the electroporation of NCI H929 multiple myeloma cells","authors":"Ayşe Kizilyer","doi":"10.24880/maeuvfd.1126466","DOIUrl":"https://doi.org/10.24880/maeuvfd.1126466","url":null,"abstract":"Multiple myeloma cell lines are difficult to transfect with non-viral nucleic acid delivery methods. While electroporation is the most efficient tool for the transfection of most hard-to-transfect cells, human multiple myeloma cells differ in their permissiveness and each cell type require different electroporation conditions for an efficient transgene delivery. In this study, various parameters for NCI H929 human multiple myeloma cells are tested to generate an optimized electroporation protocol. Findings from this paper showed that besides the voltage and capacitance settings, cell count, the cell cycle status of cells, the amount of nucleic acid and removal of death cells all impacted the electroporation efficiency and viable cell count. These results are expected to serve as a starting point and a guide for researchers.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69069074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-08DOI: 10.24880/maeuvfd.1117948
Enver Özar, M. K. Sarıbay, A. Köse, Ramazan Sertkol
The aim of this study was to evaluate the effect of selenium, β-carotene, and vitamin E administration on fertility of Awassi ewes synchronized in non-breeding season. A total of 80 multiparous Awassi ewes, aged 2 to 6 years were enrolled in the study. Intravaginal sponges containing flugeston acetate (20 mg cronolon) was inserted into vagina and allowed to remain in vagina for nine days. Ewes were randomly divided into two groups. Group I received intramuscular injections of 1 mg sodium selenite, 60 mg vitamin E, 75 mg β-carotene, and 100 mg DL-α-tocopherol acetate at sponge insertion, sponge removal, and 18 days after the introduction of ram. Group II remained as control group and received no treatment. On the removal of the sponges, 500 IU eCG and 0.075 mg D-cloprostenol were injected intramuscularly. Ram was introduced for 1 hr twice a day 24 hours after the sponge removal. Interval between sponge removal and estrus, estrus rates, conception rates, pregnancy rates, lambing rates, and fecundity rates in group I and group II were 45.30±1.71 and 43.94±1.72 hours, 79.1% and 86.6%, 66.66% and 72.41%, 54.05% and 58.33%, 100% and 100%, and 135% and 138%, respectively. There were no statistical differences between the groups (p>0.05). In conclusion, the administration of Se, β-carotene, and vitamin E in estrus synchronization protocols in non-breeding season had no positive effect on the fertility characteristics of Awassi sheep.
本研究旨在评价非繁殖期同步施用硒、β-胡萝卜素和维生素E对阿瓦西母羊育性的影响。共有80只2至6岁的多产阿瓦西母羊参加了这项研究。将含有醋酸氟孕酮(20毫克克罗龙)的阴道内海绵插入阴道，并让其在阴道内停留9天。母羊被随机分为两组。I组于海绵插入、海绵取出及公羊引入后18 d肌内注射亚硒酸钠1 mg、维生素E 60 mg、β-胡萝卜素75 mg、DL-α-生育酚醋酸酯100 mg。第二组为对照组，不进行任何治疗。取下海绵后，肌内注射500 IU eCG和0.075 mg d -氯前列醇。取下海绵24小时后，每天2次，每次1小时。1组和2组的发情间隔、发情率、受胎率、受胎率、产羔率、产仔率分别为45.30±1.71和43.94±1.72小时，分别为79.1%和86.6%、66.66%和72.41%、54.05%和58.33%、100%和100%、135%和138%。两组间比较差异无统计学意义(p < 0.05)。由此可见，在非繁殖季节，在发情同步方案中添加硒、β-胡萝卜素和维生素E对阿瓦西羊的生育特性没有积极影响。
{"title":"Effects of selenium, vitamin E, and β-carotene administration on fertility of Awassi ewes synchronized for estrus in non-breeding season","authors":"Enver Özar, M. K. Sarıbay, A. Köse, Ramazan Sertkol","doi":"10.24880/maeuvfd.1117948","DOIUrl":"https://doi.org/10.24880/maeuvfd.1117948","url":null,"abstract":"The aim of this study was to evaluate the effect of selenium, β-carotene, and vitamin E administration on fertility of Awassi ewes synchronized in non-breeding season. A total of 80 multiparous Awassi ewes, aged 2 to 6 years were enrolled in the study. Intravaginal sponges containing flugeston acetate (20 mg cronolon) was inserted into vagina and allowed to remain in vagina for nine days. Ewes were randomly divided into two groups. Group I received intramuscular injections of 1 mg sodium selenite, 60 mg vitamin E, 75 mg β-carotene, and 100 mg DL-α-tocopherol acetate at sponge insertion, sponge removal, and 18 days after the introduction of ram. Group II remained as control group and received no treatment. On the removal of the sponges, 500 IU eCG and 0.075 mg D-cloprostenol were injected intramuscularly. Ram was introduced for 1 hr twice a day 24 hours after the sponge removal. Interval between sponge removal and estrus, estrus rates, conception rates, pregnancy rates, lambing rates, and fecundity rates in group I and group II were 45.30±1.71 and 43.94±1.72 hours, 79.1% and 86.6%, 66.66% and 72.41%, 54.05% and 58.33%, 100% and 100%, and 135% and 138%, respectively. There were no statistical differences between the groups (p>0.05). In conclusion, the administration of Se, β-carotene, and vitamin E in estrus synchronization protocols in non-breeding season had no positive effect on the fertility characteristics of Awassi sheep.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69068797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-12DOI: 10.24880/maeuvfd.1066890
Ö. Akkaş, Eda Ülkü
The study was conducted on 41-second lactation Holstein cows of German origin. The shelter type is a semi-open field type and the research period is 12 months. The activities in the first 100 days of lactation per day were 451.4 ± 133.5, and in the second 100 days, it was determined at 420.78 ± 118.0. The activities are divided into 3 parts within 24 hours (at night, during the day between two milkings, and in the evening). While there was no statistical difference between days 100 and 200 of lactation, the lowest activity was recorded at night and the highest activity during the day. The conductance, milk flow, and milking duration of the milk were within the normal range in the first 100 and 200 days and no statistical difference between them could be determined. Mean daily milk yield was 28.28 ± 3.86 kg for the first 100 days and 25.15 ± 3.61 kg for the following 100 days, and the difference was found to be significant (P
{"title":"The effect of activity on some milking parameters in holstein cows","authors":"Ö. Akkaş, Eda Ülkü","doi":"10.24880/maeuvfd.1066890","DOIUrl":"https://doi.org/10.24880/maeuvfd.1066890","url":null,"abstract":"The study was conducted on 41-second lactation Holstein cows of German origin. The shelter type is a semi-open field type and the research period is 12 months. The activities in the first 100 days of lactation per day were 451.4 ± 133.5, and in the second 100 days, it was determined at 420.78 ± 118.0. The activities are divided into 3 parts within 24 hours (at night, during the day between two milkings, and in the evening). While there was no statistical difference between days 100 and 200 of lactation, the lowest activity was recorded at night and the highest activity during the day. The conductance, milk flow, and milking duration of the milk were within the normal range in the first 100 and 200 days and no statistical difference between them could be determined. Mean daily milk yield was 28.28 ± 3.86 kg for the first 100 days and 25.15 ± 3.61 kg for the following 100 days, and the difference was found to be significant (P","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69068060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Colostrum has high nutritional values, is more easily digestible than milk, and has a comparatively higher concentration of dry matter, fat and non-fat dry matter, protein and most importantly, immunoglobulin (Ig). The most important task of immunoglobulins is to neutralize pathogens and toxins through neuttalization. Lactoferrin is a protein product of the transferrin gene family with iron binding ability. Lactoferrin serves as a major component of the secondary granules of polymorphonuclear neutrophils and is produced by epithelial cells, including those in the mammary gland. Zinc acts as a cofactor and activator of more than 300 enzymes in different metabolic pathways and is known as a biologically important trace mineral. The aim of this study is to examine whether the immunoglobulin G, lactoferrin and zinc concentrations in colostrum, which is vital for newborn calves, show a decrease tendency by freezing. Fresh colostrum was given to one group (n=12) and frozen (-20 °C) colostrum (n=12) was given to one group and blood samples were collected after 32 hours. According to the results obtained, there was no statistical difference between the groups in the initial measurements of IgG, lactoferrin and zinc values in group 1 (fresh colostrum) and group 2 (frozen colostrum). The differences between the first and second measurements among themselves in both groups were found to be statistically significant. In the second measurements between the two groups, no statistical difference was found between the values of immunoglobulin G (p= 0.996), lactoferrin (p = 0.513), zinc (p = 0.605).
{"title":"Investigation of immunoglobulin G, lactoferrin and zinc levels in blood sera of calves fed fresh and frozen colostrum","authors":"Suleyman Sensoy, S. Sahinduran","doi":"10.24880/maeuvfd.992161","DOIUrl":"https://doi.org/10.24880/maeuvfd.992161","url":null,"abstract":"Colostrum has high nutritional values, is more easily digestible than milk, and has a comparatively higher concentration of dry matter, fat and non-fat dry matter, protein and most importantly, immunoglobulin (Ig). The most important task of immunoglobulins is to neutralize pathogens and toxins through neuttalization. Lactoferrin is a protein product of the transferrin gene family with iron binding ability. Lactoferrin serves as a major component of the secondary granules of polymorphonuclear neutrophils and is produced by epithelial cells, including those in the mammary gland. Zinc acts as a cofactor and activator of more than 300 enzymes in different metabolic pathways and is known as a biologically important trace mineral. The aim of this study is to examine whether the immunoglobulin G, lactoferrin and zinc concentrations in colostrum, which is vital for newborn calves, show a decrease tendency by freezing. Fresh colostrum was given to one group (n=12) and frozen (-20 °C) colostrum (n=12) was given to one group and blood samples were collected after 32 hours. According to the results obtained, there was no statistical difference between the groups in the initial measurements of IgG, lactoferrin and zinc values in group 1 (fresh colostrum) and group 2 (frozen colostrum). The differences between the first and second measurements among themselves in both groups were found to be statistically significant. In the second measurements between the two groups, no statistical difference was found between the values of immunoglobulin G (p= 0.996), lactoferrin (p = 0.513), zinc (p = 0.605).","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69072890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-30DOI: 10.24880/maeuvfd.1064458
K. Stamatova-Yovcheva, R. Dimitrov, Ö. Di̇lek, N. Tsandev, Genadi Kostadi̇nov, A. Russenov, T. Hristov
{"title":"A case of multipyramidal kidneys with smooth surface in a New Zealand white rabbit","authors":"K. Stamatova-Yovcheva, R. Dimitrov, Ö. Di̇lek, N. Tsandev, Genadi Kostadi̇nov, A. Russenov, T. Hristov","doi":"10.24880/maeuvfd.1064458","DOIUrl":"https://doi.org/10.24880/maeuvfd.1064458","url":null,"abstract":"","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47779037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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