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EFFECT OF TROPAEOLUM TUBEROSUM "MASHUA" (TROPAEOLACEAE) ON GENE EXPRESSION RELATED TO SPERMATOGENESIS IN MOUSE tropaeolum tuberosum“mashua”对小鼠精子发生相关基因表达的影响
Pub Date : 2023-09-30 DOI: 10.25176/rfmh.v23i3.5907
José Gonzales Daga, José L. Pino Gaviño, Rafael Alvis Dávila, Juan C. Francia Quiroz, Carlos Bell Cortez, Pilar V. Pino Velásquez, Betty E. Shiga Oshige
Introduction: Tropaeolum tuberosum, known as "mashua" is an Andean tuber that has both economic and nutritional value for low-income populations. It is believed that it affects male fertility because Andean men associate it with impotence and decreased fertilizing capacity. Studies done on rats fed mashua showed that there was a 45% decrease in the testosterone/dihydrotestosterone ratio. The effect of this plant on reproduction is related to its content of isothiocyanates, compounds that bind covalently to proteins, which may be directly or indirectly involved in the spermatogenic process. The purpose of this research was to evaluate the effect of the aqueous extract of "mashua" on spermatogenesis and reproductive physiology of mice. Methodology: tThe in vivo morphofunctional parameters of mouse sperm (spermatogram) were evaluated and the expression of: Cytochrome P450 17α-hydroxylase/17,20-lyase, protein that regulates acute steroidogenesis, Cyclin cyclin and Pprotamine, related to spermatogenesis, was quantified. Results: The results indicated that Aat 7, 14 and 21 days of dosing, the sperm count is affected, as well as their progressive motility (PM);, on the other hand, a delay in their maturation was observed. Regarding gene expression, no significant differences were found between the expression of the two genes studied (cCytochrome P450 17α-hydroxylase/17,20-lyase, cCyclin), Conclusion: Tthe effect of "mashua " does not occur at the level of the expression of the genes involved in spermatogenesis, but at the level of its functions as a protein.
简介:Tropaeolum tuberosum,俗称“mashua”,是一种安第斯块茎,对低收入人群具有经济和营养价值。人们认为它会影响男性的生育能力,因为安第斯男人把它与阳痿和受精能力下降联系在一起。对喂食麻花的老鼠进行的研究表明,睾酮/双氢睾酮比例降低了45%。这种植物对生殖的影响与其异硫氰酸酯的含量有关,异硫氰酸酯是一种与蛋白质共价结合的化合物,可能直接或间接地参与了生精过程。本研究旨在探讨麻花水提物对小鼠精子发生及生殖生理的影响。方法:评价小鼠精子的体内形态功能参数(精子图),定量测定与精子发生相关的细胞色素P450 17α-羟化酶/17,20-裂解酶、调节急性甾体生成的蛋白、细胞周期蛋白Cyclin Cyclin和蛋白精蛋白的表达。结果:结果表明,在给药7、14和21 d时,精子数量和进行性运动(PM)受到影响,另一方面,精子成熟延迟。在基因表达方面,cCytochrome P450 17α-羟化酶/17,20-裂解酶、cycyclinin两种基因的表达无显著差异。结论:“mashua”的作用不是发生在精子发生相关基因的表达水平上,而是发生在其作为蛋白质的功能水平上。
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引用次数: 0
Sperm DNA integrity and reproductive toxicity of lead nitrate in adult male mice 硝酸铅对成年雄性小鼠精子DNA完整性和生殖毒性的影响
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5637
José Gonzáles Daga, J. V. Vásquez Cavero, Rosmary Y. López Sam, P. P. Pino Velásquez, J. L. Pino Gaviño, Betty E. Shiga Oshige
Introduction: Lead toxicity has been linked to different diseases in humans and several evidences suggest a strong relationship with the observed damage on reproductive function in humans and rodents. Methods: we provide to mice a single dose of lead nitrate (LN) of 50mg/kg body weight. Mice were euthanized seven days post-injection with the aim of evaluating sperm to come out from the seminiferous tubules and are in transit through the epididymis. Also the TUNEL test was done to evaluate the sperm DNA fragmentation. Results: The decrease in body weight in mice treated with LN (p <0.05), suggest a toxic systemic effect. However, we didn´t find an effect on the reproductive system because the weights of the testes, epididymis, prostate and seminal vesicles were not altered (p>0.05), in the same way physiological values such as sperm concentration and motility didn´t decrease with the treatment (p> 0.05). Transit and sperm maturation in the epididymis would not be affected by the LN, and because we did not observe increased sperm DNA fragmentation in the treated group (p>0.05), sperm protamination would be fulfilling its protective role on murine genetic material avoiding genotoxic damage by LN. Conclusion: The intraperitoneal administration of 50mg/kg/pc of lead nitrate (LN) for seven days does not cause systemic toxicity or effect on spermatogenesis in mice.
引言:铅毒性与人类的不同疾病有关,一些证据表明,铅毒性与观察到的人类和啮齿动物生殖功能受损密切相关。方法:给小鼠单剂量硝酸铅(LN)50mg/kg体重。注射后7天对小鼠实施安乐死,目的是评估精子是否从曲精管中出来并通过附睾。此外,还进行了TUNEL试验来评估精子DNA的断裂。结果:LN治疗后小鼠体重下降(p 0.05),精子浓度和活力等生理指标也没有随着治疗而下降(p>0.05)。LN不会影响附睾的运输和精子成熟,因为我们没有观察到治疗组精子DNA断裂增加(p>05),精子的精蛋白作用将发挥其对小鼠遗传物质的保护作用,避免LN的基因毒性损伤。结论:50mg/kg/pc的硝酸铅(LN)腹腔给药7天,对小鼠精子发生无明显影响。
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引用次数: 0
Raptor and the importance of traffic accidents 猛禽和交通事故的重要性
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5710
Lucia Victoria Ulloa-Ordoñez, José M. Vela-Ruiz
Mr. Editor of the Revista de la Facultad de Medicina Humana, Lima, Peru I am writing to you, in relation to the review article "Association between the time of pre-hospital care and hospital mortality in victims of traffic accidents" published in 2020 Volume 20, number 01. In the aforementioned work, by M.J.G. Fernandez Sandoval et al., highlight the existence of a significant association between the time of prehospital care and mortality, with which I agree and express that it is of vital importance, even a good level of prehospital education can be applied in the specific topic of traffic accidents, since it represents an important health problem and is a priority in research.
秘鲁利马人类医学院编辑先生,我写信给你,涉及2020年发表的评论文章《交通事故受害者住院前护理时间与住院死亡率之间的关系》,第20卷,第01期。在M.J.G.Fernandez-Sandoval等人的上述工作中,强调了院前护理时间与死亡率之间存在显著联系,我同意并表示,即使是良好水平的院前教育也可以应用于交通事故的特定主题,这一点至关重要,因为它代表了一个重要的健康问题,是研究的重点。
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引用次数: 0
Nuevas indexaciones de la Revista de la Facultad de Medicina Humana: Harvard Library y DRJI 人类医学院杂志的新索引:哈佛图书馆和DRJI
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5741
J. A. De la Cruz-Vargas, Gianella Zulema Zeñas-Trujillo, D. M. Quiñones-Laveriano
The Revista de la Facultad de Medicina Humana de la Universidad Ricardo Palma (RFMH) reaches a momentous career achievement when indexed in the prestigious Harvard Library and the Directory of Research Journals Indexing (DRJI). This indexing reflects the journal's firm commitment to advancing medical science and public health through rigorous, high-quality publication.
里卡多·帕尔马大学人类医学院(RFMH)在著名的哈佛图书馆和研究期刊索引目录(DRJI)中索引时取得了重大的职业成就。该索引反映了该杂志通过严谨、高质量的出版来推进医学科学和公共卫生的坚定承诺。
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引用次数: 0
Knowledge about non-adherence to contraceptive methods Could it decrease mortality? 关于不坚持避孕方法的知识它能降低死亡率吗?
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5651
Luis Facundo Gramajo Cortez
ntroduction: In Peru, 78.1% of women between the ages of 15 and 49 use a contraceptive method, which is diminished by various factors associated with non-adherence to the contraceptive method, thus producing unwanted pregnancies and the spread of sexually transmitted diseases. Objective: To determine the factors associated with non-adherence to contraceptive methods in women of childbearing age in Peru according to ENDES 2021. Methods: Observational, analytical and cross-sectional study. The information used is found in the ENDES 2021 public database. The population of this research is made up of Peruvian women between the ages of 15 and 49. Statistical analysis was performed with the statistical program SPSS version 26.0. The qualitative variables were represented in a univariate table with frequencies and coefficients of variation. The approved statistical significance value was p <0.05 with 95% confidence intervals. Results: 43.7% of women did not adhere to the contraceptive method. There was a significant association for the variables: area of rural residence (Rpa 1.05 IC 1 – 1.1), separated marital status (Rpa 1.1, IC 1 – 1.1), widow marital status (Rpa 1.3, IC 1.1 – 1.5), cohabiting marital status ( Rpa 1.1, IC 1.1 – 1.2) , poor wealth index (Rpa 1.03, IC 0.9 – 1.0) Conclusions: The factors associated with non-adherence to contraceptive methods in women of childbearing age in Peru, which are significantly associated are: Rural area of residence, separate marital status, widow and cohabitant and have a poor wealth index.
简介:在秘鲁,78.1%的15岁至49岁的妇女使用避孕方法,但由于不遵守避孕方法的各种因素,这种方法会减少,从而导致意外怀孕和性传播疾病的传播。目的:根据ENDES 2021,确定秘鲁育龄妇女不遵守避孕方法的相关因素。方法:观察、分析和横断面研究。所使用的信息可在ENDES 2021公共数据库中找到。这项研究的人群由15岁至49岁的秘鲁女性组成。采用SPSS 26.0版统计软件进行统计分析。定性变量用单变量表表示,表中有频率和变异系数。在95%置信区间内,经批准的统计学显著性值为p<0.05。结果:43.7%的妇女没有坚持避孕方法。变量之间存在显著关联:农村居住面积(1.05印尼盾,IC1-1.1)、分居婚姻状况(1.1印尼盾,IC 1-1.1),寡妇婚姻状况(1.3印尼盾,1.1-1.5印尼盾),同居婚姻状况(1.1-1.2印尼盾),贫困财富指数(Rpa 1.03,IC 0.9-1.0)结论:秘鲁育龄妇女不遵守避孕方法的相关因素有:农村地区、单独婚姻状况、寡妇和同居者以及贫困财富指数。
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引用次数: 0
Risky eating behaviors in young adults from two communes in the Ñuble region, Chile in the COVID-19 pandemic 2019冠状病毒病大流行期间智利Ñuble地区两个社区年轻人的危险饮食行为
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5648
Javiera Saéz-Opazo, Valeria Quiroga-Salom, Monserrat Torres-Landeros, Ismael Morales-Ojeda, M. López-Espinoza, Pía Rojas-Cárdenas
Objective: To analyze risky eating behaviors in relation to nutritional status and physical activity in young adults during the COVID-19 pandemic in the communes of Chillán and Chillán Viejo. Methods: Observational and cross-sectional design. A sample of 184 young adults of both sexes, who lived in two communes in the Ñuble region (Chile) during the period of confinement due to the COVID-19 pandemic, was obtained. A personal history questionnaire and the SCOFF scale that assesses the risk of eating behavior disorders (EDs) were applied. Results: A 37.16% risk for ED was found (95% CI: 30.23 – 44.63%). The risk of eating behavior disorders was related to the variables: physical activity (p=0.01 bivariate analysis) and nutritional status (p=0.03, adjusted analysis). Conclusion: It was possible to identify a higher proportion than that recorded in the literature and the factors associated with eating disorders were those that were expected to be found.
目的:分析Chillán和Chillán Viejo社区2019冠状病毒病大流行期间年轻人危险饮食行为与营养状况和身体活动的关系。方法:采用观察和横断面设计。获得了因COVID-19大流行而在禁闭期间居住在Ñuble地区(智利)两个公社的184名男女青年的样本。采用个人病史问卷和SCOFF量表评估饮食行为障碍(EDs)的风险。结果:发生ED的风险为37.16% (95% CI: 30.23 ~ 44.63%)。饮食行为障碍的风险与以下变量相关:体力活动(p=0.01,双变量分析)和营养状况(p=0.03,调整分析)。结论:有可能确定比文献中记录的比例更高的比例,并且与饮食失调相关的因素是预期会发现的。
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引用次数: 0
University social responsibility in Latin America: Systematic review 拉丁美洲大学社会责任:系统回顾
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5653
Juana Matilde Cuba Sancho, Rosa María Contreras Gómez, Heddy Liliana Colca García, Alicia Lourdes Merino Lozano
Introduction: University social responsibility is present in the discourses of academics, but it fails to permeate the actions that lead to the transformation of the university, requiring a paradigm shift. At present there are conceptual confusions that make its promotion and transformative practice very difficult, in many universities it is confused with social projection, which reduces its transversal dimension. Objective: To analyze the scientific evidence related to university social responsibility in Latin America. Methods: Systematic review of the literature, between the months of August to December 2021; the search for articles was carried out in the databases: Scopus, Scielo, Doaj, Lilacs and Redalyc; the use of Descriptors in Health Sciences (DeCS) and the combination of the Boolean operators OR and AND were applied. Full texts in Spanish between the years 2015 and 2021 were considered. Results: 508 articles were obtained, which were submitted to the PRISMA methodology, selecting 20 articles, grouped into four categories according to the Social Responsibility Manual of the Úrsula Model: management, training, cognitive, and social participation. Conclusion: USR is an inalienable moral commitment, it is important in university management to promote sustainable development, with ethics and respect for human rights, the participation of committed actors is necessary, considering that it currently has a welfare and reductionist approach, scarce support, socialization and implementation and there is a need to unify criteria.
引言:大学社会责任存在于学术话语中,但它未能渗透到导致大学转型的行动中,需要范式转变。目前,存在着概念上的混淆,这使得它的推广和变革实践变得非常困难,在许多大学中,它与社会投射相混淆,从而降低了它的横向维度。目的:分析拉丁美洲与大学社会责任相关的科学证据。方法:对2021年8月至12月的文献进行系统回顾;在数据库中搜索文章:Scopus、Scielo、Doaj、Lilacs和Redalyc;应用了健康科学描述符(DeCS)的使用以及布尔运算符OR和and的组合。审议了2015年至2021年期间的西班牙语全文。结果:共获得508篇文章,采用PRISMA方法,选择20篇文章,根据Úrsula模型的社会责任手册分为四类:管理、培训、认知和社会参与。结论:USR是一项不可剥夺的道德承诺,在大学管理中,促进可持续发展具有重要意义,在道德和尊重人权的情况下,有义务的行为者的参与是必要的,因为它目前采用的是福利和简化主义的方法,缺乏支持、社会化和实施,并且需要统一标准。
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引用次数: 1
Fe de erratas 勘误表的信仰
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5735
J. A. De la Cruz-Vargas
"Instrument for the evaluation of climacteric and menopause in the first level of care" published in number 4, vol. 20 year 2020, p. 560-567 there are four "fe de erratas"
“在第一级护理中评估更年期和更年期的工具”发表在第4期,2020年第20卷,第560-567页,共有四个“错误”
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引用次数: 0
UNIVERSIDAD RICARDO PALMA IN THE SCIMAGO INSTITUTIONS RANKINGS 2023 里卡多·帕尔马大学在2023年Scimago机构排名中
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5734
J. A. De La Cruz-Vargas, I. Hernández-Patiño, V. Vera-Ponce, L. Correa-López, R. Pichardo-Rodríguez, Willy Ramos
Scientific research has always played a fundamental role in promoting health and the progress of society as a whole. A recent editorial published in The Lancet has discussed plausible research scenarios and outlined a vision of the future in which research could be an instrument for an "equitable and sustainable world with better health for all"
科学研究一直在促进健康和整个社会进步方面发挥着基础性作用。《柳叶刀》最近发表的一篇社论讨论了看似合理的研究场景,并概述了未来的愿景,在未来,研究可以成为一个“公平和可持续的世界,让所有人都有更好的健康”的工具
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引用次数: 0
Socio-cultural factors that influence 50-year-old users for papanicolau taking from a nasca hospital 影响50岁用户从美国国家医疗服务体系医院服用木瓜蛋白酶的社会文化因素
Pub Date : 2023-04-18 DOI: 10.25176/rfmh.v23i2.5645
Ashli Rosa Parra Zambrano, Jesús E. Talavera, Andrea P. Ramirez-Ortega, Catherine A. Suárez Egoávil
Objective: The objective of this study was to determine the sociocultural factors in 50-year-old users for Pap smears at the Ricardo Cruzado Rivarola de Nasca hospital during the period December 2021 - February 2022. Material and Methods: Observational, analytical, and prospective study. The study population is made up of 80 50-year-old users who attend the Ricardo Cruzado Rivarola hospital for Pap tests at the gynecology-obstetrics office and the sample is 67 users, selected by non-probabilistic sampling for convenience. Results: For the first analysis, in simple and multiple regression, it was found that women with high levels of knowledge had a 384% and 382% higher frequency of having ever undergone a PAP test compared to those with low levels of knowledge. Conclusions: The level of knowledge about the PAP has a relevant influence on having carried out a PAP; but also the women who had a high level were the ones who had the highest frequency of having done it. This added to the number of children; possibly because in the circles of women with children one of the topics to be discussed is about maternal health and within them the PAP.
目的:本研究的目的是确定2021年12月至2022年2月期间Ricardo Cruzado Rivarola de Nasca医院50岁巴氏涂片使用者的社会文化因素。材料和方法:观察性、分析性和前瞻性研究。研究人群由80名50岁的用户组成,他们在Ricardo Cruzado Rivarola医院的妇产科办公室接受巴氏试验,样本为67名用户,为方便起见,采用非概率抽样方法选择。结果:对于第一个分析,在简单和多元回归中,发现与知识水平低的女性相比,知识水平高的女性接受过PAP检查的频率分别高出384%和382%。结论:人民行动计划知识水平对实施人民行动计划有相关影响;但同样,高水平的女性也是做过这种事的频率最高的女性。这增加了孩子的数量;可能是因为在有孩子的妇女圈子中,讨论的主题之一是孕产妇保健以及在其中讨论的行动方案。
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引用次数: 0
期刊
Revista de la Facultad de Medicina Humana
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