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The study of the efficiency of anther culture in vitro of bread wheat varieties and hybrids (Triticum aestivum L.) 面包小麦品种及杂交种离体花药培养效率的研究
Pub Date : 2022-12-21 DOI: 10.31367/2079-8725-2022-83-6-17-22
N. V. Petrash, Е. Orlova, I. Likhenko, V. Piskarev
Anther culture is the most effective and not technically complex tool for obtaining double wheat haploids. The current study is devoted to the research of the influence of the genotype and concentration of the growth regulator 2.4-D on the efficiency of androgenesis in vitro of spring bread wheat. The purpose of the study was to estimate the efficiency of the anther culture of hybrids of the first and second generation and their parental varieties and to study its dependence on different concentrations of the growth regulator 2.4-D. There has been carried out the estimation of the traits of the F1 hybrids, the F2 population, and the parental varieties ‘Obskaya 2’ and ‘Novosibirskaya 15’ when 2.4-D was added to the induction medium at a concentration of 1 and 2 mg/l. There has been established that the F2 population and the parental variety ‘Novosibirskaya 15’ are characterized by the largest indicators. The average values were significantly surpassed by the F2 population in the traits ‘number of productive anthers per 100 anthers’ and ‘number of all regenerants per 100 anthers’ at 1 mg/l of 2.4-D. The variety ‘Novosibirskaya 15’ surpassed the average values in traits ‘number of sprouts per 100 anthers’ and ‘number of all regenerants per 100 sprouts’ at 2 mg /l of 2.4-D. The trial has shown that the best responsiveness of the anther culture for the studied genotypes was when the growth regulator 2.4-D was added to the induction medium at a concentration of 1 mg/l. The variety ‘Novosibirskaya 15’ has been characterized by good responsiveness in anther culture in vitro and could be used as a donor of valuable alleles in wheat breeding programs using DH technologies.
花药培养是获得小麦双倍体单倍体最有效且技术上不复杂的方法。本研究旨在研究生长调节剂2.4-D基因型和浓度对春面包小麦离体雄激素发生效率的影响。本研究的目的是评估一、二代杂种及其亲本品种的花药培养效率,并研究其对不同浓度生长调节剂2.4-D的依赖性。在诱导培养基中分别添加浓度为1和2 mg/l的2.4-D,对F1杂交种、F2群体和亲本品种“奥布斯卡亚2”和“新西伯利亚卡亚15”的性状进行了测定。结果表明,F2群体和亲本品种新西伯利亚15的各项指标最大。在1 mg/l 2.4-D处理下,F2群体的“每100个花药的生产花药数”和“每100个花药的所有再生花药数”显著超过了平均值。在2 mg /l 2.4-D处理下,新品种“新西伯利亚15”的“每百花药芽数”和“每百芽所有再生苗数”均超过平均值。试验结果表明,在诱导培养基中添加浓度为1 mg/l的生长调节剂2.4-D时,各基因型花药培养的响应性最佳。新品种“新西伯利亚15号”在离体花药培养中具有良好的反应性,可作为小麦DH技术育种中有价值等位基因的供体。
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引用次数: 1
Study of the collection sesame samples in the Rostov region 罗斯托夫地区收集芝麻样品的研究
Pub Date : 2022-12-21 DOI: 10.31367/2079-8725-2022-83-6-40-46
L. Zbrailova, T. Luchkina, Е. А. Krat-Kravchenko
From 2018 to 2020, there was studied a collection of sesame varieties of various ecological and geographical origin at the Don Experimental Station, a branch of the Federal State Budget Scientific Institution Federal Research Center VNIIMK. The research was carried out within the framework of State assignment No. 0684-2019-0013 ‘Development of new source material and identifying varieties and hybrids of oilseeds: sunflower, mustard, flax. Preservation of the gene pool of the main oilseeds’. The purpose of the current research was to study the morphobiological characteristics of sesame samples of various ecological and geographical origin, to study the growth and development of light- and dark-seeded groups, to identify samples with positive economically valuable traits. The color of sesame seeds is associated with their biochemical functions involved in protein and oil metabolism, as well as with the content of antioxidants (Chengqi et al., 2021). Studying collection samples by a set of traits, it is necessary to identify the most promising ones (Bashlakova, Sintsova, 2021). They must have high productivity, resistance to unfavorable conditions of the cultivation region. It is necessary to pay attention to a length of a vegetation period, a shape, location and capsules’ cracking. (Kalitskaya, Sinegovskaya et al., 2021). The current paper has presented the study results of sesame varieties from the VIR collection. There has been presented a comparative characteristic of light- and dark-seeded groups. There have been described the differences in the main economically valuable traits, phenological phases, and fatty acid composition. There has been established a range of variation between groups with different vegetation periods, fatty acid composition and other economically valuable traits. Phenological observations have shown that the range of variation in a vegetation period and plant height in the dark-seeded group was wider than in light-seeded samples. Productivity of the light-seeded group was 0.56–0.62 t/ha, which was 0.13–0.24 t/ha higher than the dark-seeded samples with 0.32–0.49 t/ha. The range of variation within the groups was 0.16 t/ha for light-seeded and 0.17 t/ha for dark-seeded samples. The oil content in the light-seeded group is also higher than the samples with dark seeds on 0.8–1.2 %, the variation range within the light-seeded group was 2.9 % and 2.5 % within the dark-seeded group. 1000-seed weight was practically at the same level between the groups and amounted to 2.9–3.0 g for light-seeded samples and 2.9–3.1 g for dark-seeded samples.
从2018年到2020年,在联邦国家预算科学机构联邦研究中心VNIIMK分支机构Don实验站研究了各种生态和地理来源的芝麻品种。该研究是在国家任务No. 0684-2019-0013“开发新来源材料和鉴定油籽品种和杂交品种:向日葵、芥菜、亚麻”的框架内进行的。主要油料种子基因库的保存。本研究旨在研究不同生态和地理来源的芝麻样品的形态生物学特征,研究浅色和深色种子类群的生长发育,鉴定具有积极经济价值的样品。芝麻的颜色与其参与蛋白质和油脂代谢的生化功能以及抗氧化剂的含量有关(Chengqi et al., 2021)。通过一组特征来研究收集样本,有必要确定最有希望的样本(Bashlakova, Sintsova, 2021)。它们必须具有较高的生产力,能抵抗种植区的不利条件。需要注意植被期的长短、形状、位置和蒴果的开裂。(Kalitskaya, Sinegovskaya等人,2021)。本文介绍了VIR收集的芝麻品种的研究结果。已经提出了一个比较特点的浅色和深色种子组。在主要的经济价值性状、物候阶段和脂肪酸组成上有不同的描述。在不同的生长期、脂肪酸组成和其他具有经济价值的性状的种群之间,已经建立了一系列的差异。物候观测表明,暗种子组的植被期和株高的变异范围比轻种子组大。轻种子组的产量为0.56 ~ 0.62 t/ha,比暗种子组的0.32 ~ 0.49 t/ha高0.13 ~ 0.24 t/ha。各组间变异幅度分别为0.16 t/ha和0.17 t/ha。轻籽组的含油量也比暗籽组高0.8 ~ 1.2%,轻籽组的含油量变化幅度为2.9%,暗籽组的含油量变化幅度为2.5%。两组间千粒重基本持平,轻种子为2.9 ~ 3.0 g,深种子为2.9 ~ 3.1 g。
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引用次数: 0
The effect of a vegetation period on protein percentage in seed of the pea collection samples 植被期对豌豆种子蛋白质含量的影响
Pub Date : 2022-12-20 DOI: 10.31367/2079-8725-2022-83-6-5-10
A. Ashiev, K. N. Khabibullin, M. Skulova
The purpose of the study was to identify the effect of the length of a vegetation period of collection samples on protein percentage in pea seed. The study was carried out in the southern part of the Rostov region on the fields of the FSBSI «ARC «Donskoy» in 2017–2020. The objects of the study were 100 collection pea samples from the N. I. Vavilov ARIPGR (VIR), including 53 leafless samples and 47 leafy ones. The longest vegetation period on average for the collection (93.8 days) was identified in 2017. The intervarietal variability of this trait, expressed by a variation coefficient, this year was 5.5 % with a minimum value of 63 days and a maximum of 101 days. In the group of varieties of the leafless type, the intervarietal variability was 6.4 % (min. 63 days; max. 100 days), in the leafy group it was 4.2 % (min. 80 days; max. 101 days). In other years, there was a reduction of a vegetation period and a decrease in its intervarietal variability due to unfavorable weather conditions in the form of a lack and shortage of precipitation against the background of elevated and high air temperatures. But they also led to the maximum accumulation of protein in seeds of collection samples, thereby showing the greatest intervarietal variability of this indicator. The calculation of the correlation coefficient showed the absence of a correlation dependence of protein percentage on length of vegetation, both on average for the collection (r = 0.03+0.10) and for the groups of leafless (r = 0.08+0.14) and foliate (r = 0.05+0.15) leaf morphotypes. The construction of graphs with errors for groups of leafy morphotypes revealed samples with a protein percentage of more than 25.0 %. In the group of the leafless morphotype, they had a vegetation period of 78–80 and 83–85 days, and in the group of the leafy morphotype, it was 77–79 days on average over the years of study.
本研究的目的是确定采集样品的植被期长度对豌豆种子中蛋白质百分比的影响。这项研究于2017年至2020年在罗斯托夫地区南部对FSBSI«ARC«Donskoy»油田进行。研究对象是来自N.I.Vavilov ARIPGR(VIR)的100个豌豆样本,包括53个无叶样本和47个有叶样本。采集的平均植被期最长(93.8天)是在2017年确定的。用变异系数表示,该性状今年的品种间变异性为5.5%,最小值为63天,最大值为101天。在无叶型品种组中,品种间变异率为6.4%(最小63天;最大100天),在有叶型品种中为4.2%(最小80天;最大101天)。在其他年份,植被期减少,品种间变异性降低,这是由于不利的天气条件,即在气温升高和高温的背景下缺乏和缺乏降水。但它们也导致了采集样本种子中蛋白质的最大积累,从而显示出该指标的最大品种间变异性。相关系数的计算表明,蛋白质百分比对植被长度的相关性不存在,无论是对集合的平均值(r=0.03+0.10),还是对无叶(r=0.08+0.14)和有叶(r=0.05+0.15)叶片形态类型的组的平均值。叶形形态类型组的带误差图的构建显示样品的蛋白质百分比超过25.0%。在无叶形态类型的组中,它们的植被期分别为78–80和83–85天,而在有叶形态类型组中,研究多年来的平均植被期为77–79天。
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引用次数: 1
Productivity, feed quality and bioenergetic estimation of cultivation of the sainfoin varieties and promising lines 红豆草品种和优良品系的生产力、饲料品质和生物能量估算
Pub Date : 2022-12-20 DOI: 10.31367/2079-8725-2022-83-6-11-16
S. Ignatiev, А. А. Regidin, G. Metlina, N. Kravchenko, K. Goryunov
The current paper has presented the estimation results of productivity, feed quality and bioenergetic efficiency of cultivation of the sainfoin varieties and promising lines. The study was carried out at the FSBSI «ARC «Donskoy» in 2019–2022. The objects of the study were 5 sainfoin varieties included in the State List and approved for use in different regions of Russia, one variety, which is currently on the State Variety Testing, and three promising lines. The standard variety was ‘Zernogradsky 2’. For 4 years the sainfoin varieties, included in the List, exceeded the standard variety on 8.3–17.4 % of green mass yield, on 5.4–10.8 % of dry matter, on 6.8–13.9 % of seeds. The variety ‘Atamansky 20’ and promising lines exceeded the standard on 17.8–23.4 %, 23.0–29.7 % and 16.7–31.0 %, respec tively. The sainfoin varieties and lines significantly exceeded the standard variety in their nutritional value. These indicators were the highest in the line ‘Sin 3/2004’ and amounted to 5.610 thousand/ha f.u., 1.59 t/ha of crude and 990 kg/ha of digestible protein. The varieties ‘Atamansky’, ‘Veles’, ‘Sudar’ and ‘Shuravi’ accumulated energy on 8.5–16.5 % more than the standard variety with green mass productivity, net energy income exceeded that of the standard variety on 9.1–17.7 %. The promising lines produced on 21.2–23.6 % more energy with vegetative mass harvesting than the standard variety. The coefficients of energy efficiency of the varieties and promising lines were 3.4–3.6. With seed yields, sainfoin varieties and promising lines accumulated energy on 6.5–31.1 % more than the standard variety. At the same time, net energy income was higher on 16.3–83.5 %, and the CEE was 1.7–2.2.
本文介绍了红豆品种和前景株系的产量、饲料品质和生物能源效率的评价结果。该研究于2019-2022年在“顿斯科伊ARC”FSBSI进行。研究对象为5个已列入国家名录并在俄罗斯不同地区批准使用的红豆素品种,1个目前正在进行国家品种试验的品种,以及3个有发展前景的品系。标准品种是“Zernogradsky 2”。4年来,列入名录的红豆品种绿色质量产量8.3 - 17.4%、干物质5.4 - 10.8%、种子6.8 - 13.9%均超过标准品种。品种“阿塔曼斯基20号”和有希望品系的超标率分别为17.8% ~ 23.4%、23.0% ~ 29.7%和16.7% ~ 31.0%。红豆品种和品系的营养价值明显超过标准品种。这些指标在“sin3 /2004”线上最高,分别为561万/公顷英尺,1.59吨/公顷原油和990公斤/公顷可消化蛋白质。‘Atamansky’、‘Veles’、‘Sudar’和‘Shuravi’的绿色质量生产力累计能量比标准品种高出8.5 ~ 16.5%,净能量收入比标准品种高出9.1 ~ 17.7%。有希望的品系在无性大量收获的情况下比标准品系多产生21.2 ~ 23.6%的能量。品种和候选品系的能量利用系数在3.4 ~ 3.6之间。在种子产量方面,红豆品种和有希望品系的能量积累比标准品种多6.5 ~ 31.1%。同时,净能源收益较高,为16.3 ~ 83.5%,CEE为1.7 ~ 2.2%。
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引用次数: 0
New hulless oat variety ‘Azil’ 无壳燕麦新品种“Azil”
Pub Date : 2022-11-16 DOI: 10.31367/2079-8725-2022-82-5-52-58
A. D. Kabashov, A. S. Kolupaeva, V. Zakharov, N. Vlasenko, O. G. Mishenkina, O. D. Yakovleva, Ya. G. Leybovich, Z. V. Filonenko, L. G. Razumovskaya, P. Polityko
The current paper has presented the study results of the new hulless oat variety ‘Azil’, which was developed by the method of individual-family selection from the hybrid population ‘Krestyansky local × ZALP’. The purpose of the current study was to identify economically valuable traits, physico-biochemical indicators of the grain of the new hulless oat variety ‘Azil’. According to the length of the vegetation period, ‘Azil’ is classified as a middle maturing variety. In the State Variety Testing, the realized productivity of the variety ‘Azil’ was 5.36 t/ha. During the study, there has been found that with intensive and high-intensity cultivation in compliance with the technological methods of protection against diseases, weeds and pests, the productivity of the variety ‘Azil’ improved in relation to the basic technology from 0.75 to 1.42 t/ha (or on 12 and 23 %, respectively). The estimation of groat properties has revealed a larger protein and oil percentage in grain of the new variety than that of the hulled and hulless standard varieties ‘Yakov’ and ‘Vyatsky’ (2.2 and 0.2 % of protein; 1.3 and 0.5 % of oil) . There has been identified high loose smut resistance of the variety ‘Azil’ (up to 6.4 % damage) on artificial and natural backgrounds. There has been established a fungi composition and mycotoxins’ content in grain of the standard oat varieties ‘Yakov’ and ‘Vyatsky’, as well as 13 hulless breeding lines of the Federal Research Center “Nemchinovka”. The variety ‘Azil’ has demonstrated an increased resistance to the accumulation of T-2 toxin. Using biochemical methods there has been found heterogeneity of the new variety, consisting of two biotypes. When conducting primary seed production, there has been recommended to use the method of individual-family selection with a double estimation of the progeny and maintaining spatial isolation with other hulless oats.
本文介绍了用个体家庭选择法从杂交群体“Krestyansky local×ZALP”中选育的无壳燕麦新品种“阿齐尔”的研究结果。本研究的目的是鉴定无壳燕麦新品种“阿齐尔”籽粒的经济价值性状、物理生化指标。根据植被期的长短,“阿齐尔”被归类为中熟品种。在国家品种试验中,阿齐尔品种的实际产量为5.36吨/公顷。在研究过程中,发现按照病虫害防治技术方法进行集约和高强度栽培,‘阿齐尔’品种的生产力相对于基础技术从0.75吨/公顷提高到1.42吨/公顷(分别提高了12%和23%)。groat特性的估计表明,新品种的谷物中蛋白质和油的百分比比有壳和无壳的标准品种“Yakov”和“Vyatsky”(蛋白质的2.2%和0.2%;油的1.3%和0.5%)更大。在人工和自然背景下,已经鉴定出“阿齐尔”品种具有较高的松散黑穗病抗性(高达6.4%的危害)。已经确定了标准燕麦品种“Yakov”和“Vyatsky”以及联邦研究中心“Nemchinovka”的13个无壳育种系的谷物中真菌成分和真菌毒素含量。“阿齐尔”品种对T-2毒素积累的抗性增强。利用生物化学方法发现了新品种的异质性,包括两个生物型。在进行初级种子生产时,建议使用个体家庭选择的方法,对后代进行双重估计,并与其他无壳燕麦保持空间隔离。
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引用次数: 0
The new maize hybrids for silage 青贮用新型杂交玉米
Pub Date : 2022-11-16 DOI: 10.31367/2079-8725-2022-82-5-66-71
G. Krivosheev, A. S. Ignatiev, D. R. Lupinoga, Yu. B. Arzhenovskaya
Maize is considered an unsurpassed silage crop, so breeding in this direction is of great relevance. The study was carried out at the FSBSI «Agricultural Research Center «Donskoy» in 2019–2021. The purpose was to study productivity and nutritional value of the new silage hybrid combinations, to identify dependencies to optimize the breeding process. In order to develop new hybrids, there was applied the method of interline hybridization using heterosis in the first generation. The objects of research were 24 hybrids of the early-maturing, middle-early and middle-maturing groups. In each group, there have been identified the new hybrid combinations that are found promising for cultivation for silage and green fodder. In the early-maturing group, a new hybrid ‘Krucha M × KV 215’ formed the largest productivity of green mass (28.2 t/ha) and dry matter (9.41 t/ha). The hybrid had 6.56 t of feed units per 1 ha, 0.48 t of digestible protein and 90.0 GJ/ha of exchange energy. In the middle-early group, a new hybrid ‘Krucha M x KV399 MV’ produced 29.4 t/ha of green mass, 10.35 t/ha of dry matter, 7.49 t of fodder units per 1 ha, 0.57 t of digestible protein, 96.14 GJ/ha of exchange energy. In the middle-maturing group, the best hybrid was a new hybrid ‘KV 399 × 9837V’, which had the highest rates among all the hybrids studied. The hybrid produced 7.88 t of fodder units per 1 ha, 0.64 t of digestible protein and 98.64 GJ/ha of exchange energy. There have been identified the signs that should be used in the development and selection of maize hybrids for silage, such as plant height, cob attachment height, number of leaves per plant, length of a vegetation period.
玉米被认为是一种无与伦比的青贮作物,因此朝着这个方向进行育种具有重要意义。该研究于2019-2021年在顿斯科伊FSBSI农业研究中心进行。目的是研究新型青贮杂交组合的生产力和营养价值,以确定相关性,从而优化育种过程。为了培育新的杂交种,在第一代中就应用了利用杂种优势进行间杂交的方法。研究对象为早熟、中早熟和中早熟群体的24个杂交种。在每一组中,都确定了新的杂交组合,这些组合被发现有可能用于青贮饲料和绿色饲料的种植。在早熟组中,新杂交种‘Krucha M×KV 215’形成了最大的绿物质产量(28.2吨/公顷)和干物质产量(9.41吨/公顷。杂交种每公顷饲料单位为6.56吨,可消化蛋白质为0.48吨,交换能为90.0 GJ/公顷。在中早期组中,新的杂交种“Krucha M x KV399 MV”产生了29.4吨/公顷的绿色物质、10.35吨/公顷干物质、7.49吨/公顷饲料单位、0.57吨可消化蛋白质和96.14 GJ/公顷交换能。在中配组中,最佳杂交种是新的杂交种“KV 399×9837V”,该杂交种在所有研究的杂交种中具有最高的比率。杂交种每1公顷产生7.88吨饲料单位,0.64吨可消化蛋白质和98.64 GJ/公顷交换能。已经确定了在开发和选择青贮玉米杂交种时应使用的标志,如株高、玉米棒附着高度、单株叶数、植被期长度。
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引用次数: 1
Comparative analysis of winter barley varieties according to productivity, its components and grain quality in the Rostov region 罗斯托夫地区冬大麦品种生产力、成分及品质比较分析
Pub Date : 2022-11-16 DOI: 10.31367/2079-8725-2022-82-5-59-65
I. Zasypkina, E. Filippov, O. A. Popova
Among the grain forage crops in the Russian Federation, barley ranks first in terms of multi-use and gross yields. However, the current level of grain production of this crop does not fully meet the needs of the livestock and food industries. Winter barley varieties are currently approved for use in the North Caucasus, Middle Volga and Nizhnevolsk regions of the Russian Federation, where its yield is 1.5–2 times higher than that of spring barley. According to the trait ‘productivity’ barley varieties of various breeding institutions have quite significant fluctuations in the regions of their cultivation, and therefore inter-station tests are carried out to determine their response. The best ones are further used in various breeding projects. The purpose of the current paper was to analyze the trait ‘productivity’ and its components and grain quality among present local and foreign winter barley varieties, in order to use the identified ones in crossings parental forms. The monitoring of varieties was carried out on the experimental plot of the Federal State Budgetary Scientific Institution “ARC «Donskoy” (2017–2019). The subjects of the study were winter barley varieties (29 samples) of local and foreign origin. Based on the results of a system analysis, there has been identified a number of varieties that have the necessary combinations of traits important for breeding, such as:– high productivity (the multi-row varieties ‘Marusya’, ‘Vivat’, ‘Foks 1’, ‘Erema’, ‘Artel’, ‘Dostoyny’ (Russia), ‘KWS-Scala’ (Germany), ‘Capten’ (France));– coarse-grained (the multi-row lines ‘KWS-117’, ‘KWS-234’, ‘KWS-History’ (Germany) and the two-row varieties ‘Explorer 3’, ‘Explorer 4’, ‘Explorer 5’, ‘Explorer 7’, ‘Explorer 3/2’, ‘Explorer 4/2’, ‘Bronskyli’ (France) with more than 50 g);– head density per 1 m2 when harvesting (the two-row varieties ‘KWS-History’ with 704 pcs/m2, ‘KWS-117’ with 710 pcs/m2 (Germany), ‘Explorer 8’ with 739 pcs/m2, ‘Explorer 3/2’ with 759 pieces/m2, ‘Wintwalt’ with 847 pieces/m2 (France));– number of grains per head (the multi-row varieties ‘Marusya’ with 57.9 pcs, ‘Andryusha’ with 55.8 pcs (Russia), ‘Capten’ with 55.6 pcs (France) and two-row varieties ‘Explorer 3’ with 26.0 pcs., ‘Explorer 5’ with 25.9 pcs., ‘Bronskyli’ with 25.8 pcs (France);– stable protein percentage in grain less than 11 % (the two-row varieties ‘KWS-History’ with 10.3 %, ‘KWS-234’ with 10.7 % (Germany) and ‘Explorer 3/2’ with 10.3 % (France).
在俄罗斯联邦的粮食饲料作物中,大麦的综合用途和总产量均居首位。然而,目前这种作物的粮食生产水平不能完全满足畜牧业和食品工业的需要。冬大麦品种目前已被批准用于俄罗斯联邦的北高加索、中伏尔加河和下涅沃尔斯克地区,其产量比春大麦高1.5-2倍。根据各育种机构大麦品种的“生产力”性状,在其栽培区域有相当大的波动,因此进行了站间试验以确定其响应。最好的品种可进一步用于各种育种项目。本文对国内外现有冬大麦品种的“生产力”性状及其组成和籽粒品质进行了分析,以便在杂交亲本中使用鉴定的品种。品种监测是在联邦国家预算科学机构“ARC”的试验田进行的(2017-2019年)。本研究的对象是来自本地和国外的冬大麦品种(29个样本)。根据系统分析的结果,已经确定了一些具有育种所需重要性状组合的品种,例如:高产(多行品种‘Marusya’、‘Vivat’、‘Foks 1’、‘Erema’、‘Artel’、‘Dostoyny’(俄罗斯)、‘KWS-Scala’(德国)、‘Capten’(法国));粗粒(多行品种‘KWS-117’、‘KWS-234’、‘KWS-History’(德国)和两行品种‘Explorer 3’、‘Explorer 4’、‘Explorer 5’、‘Explorer 7’)。-收获时每平方米的穗密度(两行品种“KWS-History”为704个/平方米,“KWS-117”为710个/平方米(德国),“Explorer 8”为739个/平方米,“Explorer 3/2”为759个/平方米,“Wintwalt”为847个/平方米(法国));-每穗粒数(多行品种“Marusya”为57.9个,“Andryusha”为55.8个/平方米(俄罗斯);“Capten”(55.6件)(法国)和“Explorer 3”(26.0件)。,“Explorer 5”(25.9台)。-籽粒稳定蛋白质含量低于11%(两行品种“KWS-History”为10.3%,“KWS-234”为10.7%(德国),“Explorer 3/2”为10.3%(法国))。
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引用次数: 1
Identification of the allelic state of the flood tolerance gene (Sub-1A) in rice breeding samples 水稻抗洪基因(sub1a)等位基因状态的鉴定
Pub Date : 2022-11-16 DOI: 10.31367/2079-8725-2022-82-5-15-19
N. Vozhzhova, O. Zhogaleva, A. Dubina, P. Kostylev
Rice is one of the most important crops in the world. Rice crops provide food for many people in Asia and the Pacific. In Russia, the production of this crop is concentrated in several regions, one of which is the Rostov region. The trend towards the greening of agriculture throughout the world and in Russia is becoming more and more stable. In order to combat weeds using an environmentally friendly herbicide-free technology, it is necessary to increase the water layer on simultaneous surviving rice plants. For such a strategy to work, it is necessary to develop varieties that are resistant to flooding. The purpose of the current study was to identify the allelic state of the Sub1A flood resistance gene in breeding rice samples. The objects of the study were 90 rice samples of the sixth generation, identified in hybrids obtained from crossing Russian varieties with gene donor varieties. The analysis was carried out by such methods as DNA extraction using the “DNA-Extran-3” commercial kit (Sintol, Russia), classical PCR with the specialized diagnostic marker Sub1A203, separation of amplicons by electrophoresis in 2 % agarose gels, analysis of electrophoregrams in the software Bio-Rad ImageLab 6.0.1. As a result of the analysis, there was identified the allelic state of the Sub1A gene in 90 rice samples. A significant number of samples carried a non-functional recessive allele, the presence of the gene was not established in 19 samples. The Sub1A gene was in the heterozygous state in 9 samples. The presence of a functional dominant allele of the target gene was identified n 5 breeding samples. There has been recommended to use these samples in breeding work aimed at developing new rice varieties resistant to prolonged flooding.
水稻是世界上最重要的农作物之一。水稻作物为亚洲和太平洋地区的许多人提供食物。在俄罗斯,这种作物的生产集中在几个地区,其中之一是罗斯托夫地区。全世界和俄罗斯的农业绿色化趋势正变得越来越稳定。为了使用环境友好的无除草剂技术来对抗杂草,有必要增加同时存活的水稻植株的水层。为了使这一策略发挥作用,有必要开发抗洪水的品种。本研究的目的是鉴定水稻Sub1A抗洪基因的等位基因状态。该研究的对象是90份第六代水稻样本,这些样本是由俄罗斯品种与基因供体品种杂交获得的。采用“DNA-外扩-3”商业试剂盒(俄罗斯Sintol)提取DNA,使用专用诊断标记物Sub1A203进行经典PCR,用2%琼脂糖凝胶电泳分离扩增子,用Bio-Rad ImageLab 6.0.1软件分析电泳图。通过分析,在90份水稻样品中确定了Sub1A基因的等位基因状态。大量样品携带非功能性隐性等位基因,19个样品未确定该基因的存在。在9份样本中,Sub1A基因处于杂合状态。在5个育种样品中鉴定出目的基因的功能性显性等位基因的存在。有人建议将这些样品用于培育抗长期洪水的水稻新品种的育种工作中。
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引用次数: 0
Results of estimating adaptability of sainfoin varieties in the south of the Rostov region 红豆品种在罗斯托夫南部地区的适应性评估结果
Pub Date : 2022-11-16 DOI: 10.31367/2079-8725-2022-82-5-33-38
S. Ignatiev, A. Regidin, N. Kravchenko, K. Goryunov
The purpose of the study was to estimate such adaptability parameters, as a linear regression coefficient, stress resistance, genetic flexibility and stability coefficient, according to the traits “green mass productivity” and “dry matter productivity”. The objects of the study were 6 sainfoin varieties developed by the ARC “Donskoy”. The variety ‘Zernogradsky 2’ was taken as the standard one. Sowing was carried out in the spring with the SSFC-7 seeder in its pure form, without a cover crop. A seeding rate was 5 million germinated seeds per 1 ha. The area of the plot was 20 m2, with fourfold repetition. The estimation according to the indicators of ecological adaptability was performed according to the S. A. Eberchart and W. A. Russel (1966) as presented by V. A. Zykina et al. (2011), calculation of stress resistance and genetic flexibility was established according to A.A. Goncharenko (2005). The results of the analysis of variance have shown a significant influence of factor B (conditions) both on the variability of productivity of green mass (79.12 %) and dry matter (75.38 %). All the studied sainfoin varieties according to the years and sowing significantly exceeded the indicators of the standard variety. The most productive of them, the new variety ‘Atamansky 20’, formed on average 30.9 t/ha of green mass and 8.3 t/ha of dry mass, with fluctuations over the years, respectively, 24.8–44.9 t/ha and 6.4 –12.1 t/ha. The sainfoin varieties differ in the response of green and dry mass productivity to changing environmental conditions. The varieties ‘Zernogradsky 2’, ‘Atamansky’ and ‘Atamansky 20’ demand to improve conditions when cultivated for green feed and hay (bi > 1), while the varieties ‘Veles’, ‘Sudar’ and ‘Shuravi’ respond less well to improved environmental conditions and are more adaptable (bi < 1). The varieties ‘Veles’, ‘Sudar’ and ‘Shuravi’ are highly resistant to stress, while the varieties Zernogradsky 2’, ‘Atamansky’ and ‘Atamansky 20’ have a narrower range of adaptive capabilities of stress resistance. A higher correspondence between a genotype and environmental factors and high genetic flexibility were found in the variety ‘Atamansky 20’.
本研究的目的是根据“绿色质量生产力”和“干物质生产力”的性状,估计线性回归系数、抗逆性、遗传柔韧性和稳定性等适应性参数。本研究以“顿斯科伊”农业研究中心培育的6个红豆品种为研究对象。品种“Zernogradsky 2”被作为标准品种。播种在春季进行,SSFC-7播种机在其纯形式,没有覆盖作物。播种率为每公顷500万颗发芽种子。地块面积为20平方米,重复四次。根据生态适应性指标进行估算,参照S. A. Eberchart和W. A. Russel(1966)和V. A. Zykina et al.(2011),计算抗逆性和遗传灵活性,参照A.A. Goncharenko(2005)。方差分析结果表明,因子B(条件)对绿质生产力变异率(79.12%)和干物质生产力变异率(75.38%)均有显著影响。所有红豆品种按年份和播种情况均显著超过标准品种的指标。其中产量最高的新品种“Atamansky 20”,平均绿重30.9 t/ha,干重8.3 t/ha,历年波动分别为24.8 ~ 44.9 t/ha和6.4 ~ 12.1 t/ha。红豆品种的绿色和干质量生产力对变化的环境条件的响应不同。品种“Zernogradsky 2”、“Atamansky”和“Atamansky 20”在绿色饲料和干草栽培时需要改善条件(bi bbbb1),而品种“Veles”、“Sudar”和“Shuravi”对改善环境条件的反应较差,适应性较强(bi < 1)。品种“Veles”、“Sudar”和“Shuravi”具有较强的抗逆性,而品种“Zernogradsky 2”、“Atamansky”和“Atamansky 20”的抗逆性适应能力范围较窄。品种“Atamansky 20”的基因型与环境因子的对应关系较高,遗传灵活性较高。
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引用次数: 0
Strong varieties are the basis to produce high-quality processing products of bread wheat grain 优良的品种是生产优质面包小麦加工产品的基础
Pub Date : 2022-11-16 DOI: 10.31367/2079-8725-2022-82-5-39-46
I. V. Pakhotina, E. Ignatieva, I. Belan, L. Rosseeva, L. T. Soldatova
There have been studied new strong varieties of spring bread wheat of different maturity groups (‘Tarskaya 12’, ‘Omskaya 44’ and ‘Omskaya 42’) in comparison with the standards (‘Pamyati Azieva’, ‘Duet’ and ‘Element 22’) and foreign varieties in the conditions of the south of Western Siberia. The purpose of the study was to identify the advantage of the studied wheat varieties recommended for cultivation in the Omsk region to produce grain with high baking properties. The study was carried out based on the selection crop rotation of the laboratory for spring bread wheat breeding in the nursery of Competitive Variety Testing, as well as the department of seed production when sown on fallow (intense background) in the zone of the southern forest-steppe of the Omsk region of the FSBSI “Omsk ARC”. There has been conducted a bakery quality estimation and qualification of samples according to the methodologies and classification standards recommended by the State Commission for Variety Testing of Agricultural Plants. There was identified an advantage of the medium-early variety ‘Tarskaya 12’ according to the formation of gluten amount in grain (+3.2...4.2 %), dough dilution (–34...50 u.f.), dough resilience (+24...43 mm) and flour strength (+113...188 u); middle maturing variety ‘Omskaya 44’ on the formation of dough resilience, flour strength in comparison with the standards. The middle-late variety ‘Omskaya 42’ was distinguished by high baking characteristics in comparison with the standard ‘Element 22’. A comparative analysis of the technological properties of new varieties and foreign breeding has revealed the advantage of the studied domestic varieties in formation of protein in grain and flour strength.
在西伯利亚西部南部的条件下,研究了不同成熟度组的春小麦新品种(‘Tarskaya 12’、‘Omskaya 44’和‘Omskaya 42’),并与标准品种(‘Pamyati Azieva’、‘Duet’和‘Element 22’)和外国品种进行了比较。该研究的目的是确定在鄂木斯克地区推荐种植的小麦品种的优势,以生产具有高烘焙性能的谷物。该研究是在FSBSI“鄂木斯克ARC”鄂木斯克地区南部森林草原区域的春小麦育种实验室选择作物轮作(高背景)以及种子生产部门的基础上进行的。根据国家农业植物品种检验委员会推荐的方法和分类标准,对烘焙样品进行了质量评估和鉴定。根据籽粒中面筋形成量(+3.2 ~ 4.2 %)、面团稀释度(-34 ~ 4.2 %),确定了中早品种“塔斯卡雅12”的优势。50 u.f),面团弹性(+24…43毫米)和面粉强度(+113…188 u);中熟品种‘奥姆斯卡娅44号’对形成面团的回弹性、面粉强度进行了达标比较。与标准品种“Element 22”相比,中后期品种“Omskaya 42”的烘焙特性较高。通过与国外选育品种技术特性的比较分析,揭示了国产品种在籽粒蛋白质形成和面粉强度方面的优势。
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引用次数: 2
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Zernovoe khoziaistvo Rossii
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