Pub Date : 2022-03-01DOI: 10.21608/mjapam.2022.228715
,. H. A. Ammar, ,. S. H. El-Helaly, T. A. Abdel-Rahman
: The aim of this study was to evaluate four the antifungal activity of aqueous plant extracts ( Laurus nobilis , Dianthus caryophyllus , Cinnamomum verum , and Thymus vulgaris ) as an alternatives to chemical fungicides (Imazalil and Thiabendazole) against Alternaria alternata which causing black spot of sugar mango fruits. Antifungal activities of plant extracts and chemical fungicideswere tested both in vitro and in vivo conditions. The in vitro assay showed maximum inhibition of radial mycelial growth of A. alternata (100%) by D. caryophyllus extract, at concentrations of 2.5, 5, and 10% Similarly, the in-vivo assay showed a marked reduction in lesion diameter (2.5, 1.5, and 0.9 mm) and decreasing disease severity by 95.46, 97.04 and 98.22 %, respectively on the mango fruits treated with this extract. While the in vitro assay with Imazalil fungicide showed inhibition of radial mycelial growth of A. alternate by 82.13, 85.22, and 89.35%, respectively at 50, 100, and 200 ppm, respectively and reduced disease severity by 86.37, 88.90, and 93.43%, respectively. The residual activity of post-harvest treatment with imazalil (IMZ) and thiabendazole (TBZ) was studied at different dip lengths and different cold storage times on mango fruit. IMZ and TBZ residue decreased with the number of cold storage days for all treatments and the residue increased with the duration of dipping for all treatments. Both fungicides residues were lower than MRL set by codex and EU Pesticides Database.
{"title":"EFFICACY OF SOME PLANT EXTRACTS AND FUNGICIDES TO CONTROL ALTERNARIA BLACK SPOTS CAUSED BY ALTERNARIA ALTERNATA ON POSTHARVEST MANGO FRUITS IN EGYPT","authors":",. H. A. Ammar, ,. S. H. El-Helaly, T. A. Abdel-Rahman","doi":"10.21608/mjapam.2022.228715","DOIUrl":"https://doi.org/10.21608/mjapam.2022.228715","url":null,"abstract":": The aim of this study was to evaluate four the antifungal activity of aqueous plant extracts ( Laurus nobilis , Dianthus caryophyllus , Cinnamomum verum , and Thymus vulgaris ) as an alternatives to chemical fungicides (Imazalil and Thiabendazole) against Alternaria alternata which causing black spot of sugar mango fruits. Antifungal activities of plant extracts and chemical fungicideswere tested both in vitro and in vivo conditions. The in vitro assay showed maximum inhibition of radial mycelial growth of A. alternata (100%) by D. caryophyllus extract, at concentrations of 2.5, 5, and 10% Similarly, the in-vivo assay showed a marked reduction in lesion diameter (2.5, 1.5, and 0.9 mm) and decreasing disease severity by 95.46, 97.04 and 98.22 %, respectively on the mango fruits treated with this extract. While the in vitro assay with Imazalil fungicide showed inhibition of radial mycelial growth of A. alternate by 82.13, 85.22, and 89.35%, respectively at 50, 100, and 200 ppm, respectively and reduced disease severity by 86.37, 88.90, and 93.43%, respectively. The residual activity of post-harvest treatment with imazalil (IMZ) and thiabendazole (TBZ) was studied at different dip lengths and different cold storage times on mango fruit. IMZ and TBZ residue decreased with the number of cold storage days for all treatments and the residue increased with the duration of dipping for all treatments. Both fungicides residues were lower than MRL set by codex and EU Pesticides Database.","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"30 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127096167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-01DOI: 10.21608/mjapam.2022.222918
,. Said, ,. B. M. El Defrawy
{"title":"EFFECT OF DIFFERENT DIETS ON SOME LARVAL BODY CHARACTERISTICS AND FOOD UTILIZATION EFFICIENCY OF TENEBRIO MOLITOR (COLEOPTERA: TENEBRIONIDAE)","authors":",. Said, ,. B. M. El Defrawy","doi":"10.21608/mjapam.2022.222918","DOIUrl":"https://doi.org/10.21608/mjapam.2022.222918","url":null,"abstract":"","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"464 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131581475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-01DOI: 10.21608/mjapam.2022.222915
,. B. M. El Defrawy, ,. Said
{"title":"PREDATION EFFICIENCY AND SOME BIOLOGICAL ASPECTS OF THE PREDATOR CHRYSOPERLA CARNEA (STEPHENS) (NEUROPTERA: CHRYSOPIDAE) LARVAE FEEDING ON DIFFERENT EGG PREYS UNDER LABORATORY CONDITIONS","authors":",. B. M. El Defrawy, ,. Said","doi":"10.21608/mjapam.2022.222915","DOIUrl":"https://doi.org/10.21608/mjapam.2022.222915","url":null,"abstract":"","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"97 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130981887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/mjapam.2021.212692
M. Sweelam, M. Abokora
{"title":"STUDIES ON THE BIOLOGICAL CONTROL OF NEMATODES","authors":"M. Sweelam, M. Abokora","doi":"10.21608/mjapam.2021.212692","DOIUrl":"https://doi.org/10.21608/mjapam.2021.212692","url":null,"abstract":"","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134284197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/mjapam.2021.214230
E. Mousa, M. Mahdy, Hanaa A. Abo-Koura
{"title":"BIOLOGICAL CONTROL OF ROOT-KNOT NEMATODES USING SOME ANTAGONISTIC MICROORGANISMS ON TOMATO","authors":"E. Mousa, M. Mahdy, Hanaa A. Abo-Koura","doi":"10.21608/mjapam.2021.214230","DOIUrl":"https://doi.org/10.21608/mjapam.2021.214230","url":null,"abstract":"","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133965437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.21608/mjapam.2021.207655
Nehal O. Swelam
An ecological study was conducted to elucidate the peaks of the white grubs’ larvae infesting strawberry roots along one year from May 2020 to April 2021. Furthermore, feeding and control study was applied under laboratory conditions. Results revealed that May and September months recorded 20 larvae per root of strawberry plant as the highest numbers of larvae under 17-38°C and 32-61 RH%. The larvae of the Pentodon bispinosus were reared on three vegetable roots; however, the grubs have a wide range of host families. The larvae of the P. bispinosus prefer the roots of the Strawberry plants recording the highest weights as 7 ± 0.6 g roots/day. Bioinsecticides were tested to determine the most effective agents against white grubs, from the results, the third stage of grubs have resistance against Abamectin 3.6 % and Chitosan. Abamectin 3.6% EC recorded 30% reduction in larvae population after 3 days of treatment decreased to 10% after 30 days, on contrary, Chitosan recorded zero reduction.
{"title":"ECOLOGY AND BIOCONTROL STUDY ON THE WHITE GRUBS, PENTODON BISPINOSUS INFESTING STRAWBERRY ROOTS","authors":"Nehal O. Swelam","doi":"10.21608/mjapam.2021.207655","DOIUrl":"https://doi.org/10.21608/mjapam.2021.207655","url":null,"abstract":"An ecological study was conducted to elucidate the peaks of the white grubs’ larvae infesting strawberry roots along one year from May 2020 to April 2021. Furthermore, feeding and control study was applied under laboratory conditions. Results revealed that May and September months recorded 20 larvae per root of strawberry plant as the highest numbers of larvae under 17-38°C and 32-61 RH%. The larvae of the Pentodon bispinosus were reared on three vegetable roots; however, the grubs have a wide range of host families. The larvae of the P. bispinosus prefer the roots of the Strawberry plants recording the highest weights as 7 ± 0.6 g roots/day. Bioinsecticides were tested to determine the most effective agents against white grubs, from the results, the third stage of grubs have resistance against Abamectin 3.6 % and Chitosan. Abamectin 3.6% EC recorded 30% reduction in larvae population after 3 days of treatment decreased to 10% after 30 days, on contrary, Chitosan recorded zero reduction.","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113935774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.21608/mjapam.2021.207654
Nehal O. Swelam, M. A. Tahon
Seasonal fluctuations of wheat aphid, Rhopalosiphum padi L., infesting five wheat varieties, were investigated under field conditions along 2020/2021 season at Menoufia governorate, as well as, biological and biocontrol studies on this pest were conducted, moreover, residual of pesticides were determined in seeds, straw and soil of treated wheat plants. As for the population density of R. padi, the highest numbers were recorded in March 2021 on Gemiza 11 variety. The life cycle of R. padi lasts 10.6 days ± 0.5 , the adult viviparous female lived 12.2 ± 0.4 days spends two days without putting larvae, put 28.4 ± 1.3 individuals during the reproductive period (10.2± 0.4) days, the life span lasts 20.8 ± 0.8 days. Abamectin 3.6 % EC (double dose) and Congest 15% SC were effective in reducing the number of aphids per leaf, recording 46.67100% reduction and recording residues in wheat straw and wheat seeds less than the maximum residues limits. The tested treatments did not affect the germination process. The tested bio agents as nonconventional insecticide could be considered suitable for aphid control and safe for human and environment. It could be recommended the use of Abamectin and Imidacloprid in integrated aphid management programs.
{"title":"BIOLOGICAL AND CONTROL STUDIES ON RHOPALOSIPHUM PADI INFESTING WHEAT PLANTS, TRITICUM AESTIVUM AT MENOUFIA GOVERNORATE, EGYPT","authors":"Nehal O. Swelam, M. A. Tahon","doi":"10.21608/mjapam.2021.207654","DOIUrl":"https://doi.org/10.21608/mjapam.2021.207654","url":null,"abstract":"Seasonal fluctuations of wheat aphid, Rhopalosiphum padi L., infesting five wheat varieties, were investigated under field conditions along 2020/2021 season at Menoufia governorate, as well as, biological and biocontrol studies on this pest were conducted, moreover, residual of pesticides were determined in seeds, straw and soil of treated wheat plants. As for the population density of R. padi, the highest numbers were recorded in March 2021 on Gemiza 11 variety. The life cycle of R. padi lasts 10.6 days ± 0.5 , the adult viviparous female lived 12.2 ± 0.4 days spends two days without putting larvae, put 28.4 ± 1.3 individuals during the reproductive period (10.2± 0.4) days, the life span lasts 20.8 ± 0.8 days. Abamectin 3.6 % EC (double dose) and Congest 15% SC were effective in reducing the number of aphids per leaf, recording 46.67100% reduction and recording residues in wheat straw and wheat seeds less than the maximum residues limits. The tested treatments did not affect the germination process. The tested bio agents as nonconventional insecticide could be considered suitable for aphid control and safe for human and environment. It could be recommended the use of Abamectin and Imidacloprid in integrated aphid management programs.","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124192468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.21608/mjapam.2021.202651
M.A.I. Abdel Azeim, M.M.I. El-Refaay, A. Abdelaal, H. Sharaf El-Din
Population dynamics of Varroa destructor , Anderson and Trueman were studied along the autumn season of 2018 to the summer season of 2019 in 20 Apis mellifera , shaded or unshaded colonies, located in Dokki (Giza). The number of bees, the amount of open and capped brood , daily natural mortality, level of infestation of adult bees, and level of infestation of the brood, was monitored. The obtained results showed that the infestation with Varroa mite was high in winter and autumn and was low in spring and summer on brood and on adult worker bees. Relative humidity and outdoor temperature leading to great danger to bee colonies in dearth period. So, early mite control is very important. Successive brood cycles allow the population growth of Varroa, while the absence of brood during the summer months has the opposite effect of reducing populations of Varroa.
{"title":"EFFECT OF ENVIRONMENTAL FACTORS ON THE INVENTORY AND POPULATION OF VARROA MITE, VARROA DESTRUCTOR IN HONEY BEE APIS MELLIFERA COLONIES IN DOKKI, Giza, EGYPT","authors":"M.A.I. Abdel Azeim, M.M.I. El-Refaay, A. Abdelaal, H. Sharaf El-Din","doi":"10.21608/mjapam.2021.202651","DOIUrl":"https://doi.org/10.21608/mjapam.2021.202651","url":null,"abstract":"Population dynamics of Varroa destructor , Anderson and Trueman were studied along the autumn season of 2018 to the summer season of 2019 in 20 Apis mellifera , shaded or unshaded colonies, located in Dokki (Giza). The number of bees, the amount of open and capped brood , daily natural mortality, level of infestation of adult bees, and level of infestation of the brood, was monitored. The obtained results showed that the infestation with Varroa mite was high in winter and autumn and was low in spring and summer on brood and on adult worker bees. Relative humidity and outdoor temperature leading to great danger to bee colonies in dearth period. So, early mite control is very important. Successive brood cycles allow the population growth of Varroa, while the absence of brood during the summer months has the opposite effect of reducing populations of Varroa.","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134385082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/mjapam.2021.198682
G. A. Ahmed, A. El-sisi, M. E. Selim
The aim of this present study is to evaluate the antimicrobial activities of three plants essential oils, Nigella (Nigella sativa), Eucalyptus (Cinnamomum camphora) and clove (Syzygium aromaticum) against plant pathogenic bacteria Xanthomonas vesicatoria and their influences on controlling the bacterial spot disease of tomato plants. The chemical composition of the oils was calculated by gas chromatograph/mass spectrometer (GC/MS) analysis. The results revealed that the major constituents of Nigella oil were fatty acid i.e. oleic acid (4.09%%), Fumaric acid (0.13%), Palmitic acid (4.95-1.65 %), Octadec-9-enoic acid (0.53%), myristic acid (0.78%), Cyclohexadecane (0.01%) and Hexanedioic acid bis (2-ethylhexyl) ester (75.02%). In addition, the main components of Eucalyptus oil were D-camphor (40.01%), linalool (20.57%), and cineole (10.86%). The chemical analysis showed also that clove oil contained eugenol (22.94%), eugenyl acetate (16.65%), caryophyllene (10.37 %) and 2-Pyridineethanol (6.08 %). In in vitro bioassay against X.anthomonas vesicatoria, the results demonstrated that among the three tested plant oils, Eucalyptus oil (10 %) recorded the maximum value was (1.73 cm) bacterial inhibition zone followed by Clove oil (1.50 cm). Results also showed spraying plants with the tested essential oils two days before bacterial inoculation recorded the lowest disease index comparing to the treatment where plants were treating with essential oils two days after bacterial inoculation. The obtained results also clearly illustrated that, Peroxidase (PO), polyphenoloxidase PPO and chitinase activities increased as a result of spraying tomato plants with the tested oils. Moreover, protein analysis confirmed that new protein bands with low molecular weight had a progressive relationship with reduction of bacterial spot disease severity on plants treated with the tested oils.
{"title":"GC-MS ANALYSIS OF THREE PLANTS ESSENTIAL OILS AND THEIR EFFECT ON BACTERIAL SPOT DISEASE OF TOMATO","authors":"G. A. Ahmed, A. El-sisi, M. E. Selim","doi":"10.21608/mjapam.2021.198682","DOIUrl":"https://doi.org/10.21608/mjapam.2021.198682","url":null,"abstract":"The aim of this present study is to evaluate the antimicrobial activities of three plants essential oils, Nigella (Nigella sativa), Eucalyptus (Cinnamomum camphora) and clove (Syzygium aromaticum) against plant pathogenic bacteria Xanthomonas vesicatoria and their influences on controlling the bacterial spot disease of tomato plants. The chemical composition of the oils was calculated by gas chromatograph/mass spectrometer (GC/MS) analysis. The results revealed that the major constituents of Nigella oil were fatty acid i.e. oleic acid (4.09%%), Fumaric acid (0.13%), Palmitic acid (4.95-1.65 %), Octadec-9-enoic acid (0.53%), myristic acid (0.78%), Cyclohexadecane (0.01%) and Hexanedioic acid bis (2-ethylhexyl) ester (75.02%). In addition, the main components of Eucalyptus oil were D-camphor (40.01%), linalool (20.57%), and cineole (10.86%). The chemical analysis showed also that clove oil contained eugenol (22.94%), eugenyl acetate (16.65%), caryophyllene (10.37 %) and 2-Pyridineethanol (6.08 %). In in vitro bioassay against X.anthomonas vesicatoria, the results demonstrated that among the three tested plant oils, Eucalyptus oil (10 %) recorded the maximum value was (1.73 cm) bacterial inhibition zone followed by Clove oil (1.50 cm). Results also showed spraying plants with the tested essential oils two days before bacterial inoculation recorded the lowest disease index comparing to the treatment where plants were treating with essential oils two days after bacterial inoculation. The obtained results also clearly illustrated that, Peroxidase (PO), polyphenoloxidase PPO and chitinase activities increased as a result of spraying tomato plants with the tested oils. Moreover, protein analysis confirmed that new protein bands with low molecular weight had a progressive relationship with reduction of bacterial spot disease severity on plants treated with the tested oils.","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133348911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-01DOI: 10.21608/mjapam.2021.193754
M. Selim, H. Mahdy, G. A. Ahmed, A. Makhlouf
Wilting disease of tomato caused by Fusarium oxysporum f. sp. lycopersici, (FOL) is one of the most destructive diseases. This disease causes significant yield losses in Egypt. Ten Fusarium isolates were isolated from wilted tomato plants growing in El-Menoufia Governorate during 2020 growing season. The most virulent isolate (isolate No. 6) was tested for its virulence on ten tomato cultivars under greenhouse conditions. The cultivars were significantly varied in their susceptibility toward the tested virulent isolate. The most susceptible cultivar was Carmen F1cv as it recorded the highest value of infection (100% and 96%) in seeds and seedlings experiments, respectively. On the other hand the results showed that Diamond Arwa cultivar was the most resistant tested cultivar where zero infection percentage was recorded on both seeds and seedling experiments. The chemical analysis using HPLC was conducted with the most susceptible tomato cultivar (Carmen) and the most resistant one (Diamond Arwa) one and three weeks post inoculation with pathogenic isolate of Fusarium. The results revealed that the accumulation of total phenolic compounds either in shoots or roots was higher three weeks after inoculation than one week post inoculation within the two tested cultivars. Remarkable that roots and shoots of most resistant cultivar tested recorded more total phenol concentrations rather than the susceptible one. Significant differences were detected among the resistant and the susceptible cultivars tested with the compounds detected at 21.2m; 26,2m and 29.1 minute in both shoots and roots three weeks after pathogen inoculation which suggesting potential role of these particular chemicals in resistance mechanism toward Fusarium wilt disease within resistant tomato cultivars.
番茄枯萎病(Fusarium oxysporum f. sp. lycopersici, FOL)是番茄最具破坏性的病害之一。这种疾病在埃及造成重大的产量损失。从El-Menoufia省2020年生长季生长的枯萎番茄植株中分离出10株镰刀菌。在温室条件下，对毒力最强的分离物(6号分离物)在10个番茄品种上进行了毒力测定。不同品种对所测毒株的敏感性差异显著。在种子和幼苗试验中，卡门F1cv的侵染率最高，分别达到100%和96%。另一方面，在种子和幼苗试验中，菱形Arwa品种的侵染率均为零，是抗侵染率最高的品种。用高效液相色谱法(HPLC)对最敏感的番茄品种卡门(Carmen)和最抗性的番茄品种钻石Arwa (Diamond Arwa)接种镰刀菌病原菌1周和3周后进行化学分析。结果表明，接种后3周，两个品种的茎部和根部总酚类物质积累量均高于接种后1周。值得注意的是，大多数抗性品种的根和芽记录的总酚浓度高于敏感品种。在21.2m处检测到的抗感品种间差异显著;在接种后3周内，这些化学物质在番茄的茎部和根部分别发生了26、2m和29.1 min的变化，表明这些化学物质在抗病番茄品种对枯萎病的抗性机制中可能起作用。
{"title":"RELATION BETWEEN FUSARIUM WILT DISEASE AND ACCUMULATION OF PHENOLIC COMPOUNDS WITHIN RESISTANT AND SUSCEPTIBLE TOMATO CULTIVARS","authors":"M. Selim, H. Mahdy, G. A. Ahmed, A. Makhlouf","doi":"10.21608/mjapam.2021.193754","DOIUrl":"https://doi.org/10.21608/mjapam.2021.193754","url":null,"abstract":"Wilting disease of tomato caused by Fusarium oxysporum f. sp. lycopersici, (FOL) is one of the most destructive diseases. This disease causes significant yield losses in Egypt. Ten Fusarium isolates were isolated from wilted tomato plants growing in El-Menoufia Governorate during 2020 growing season. The most virulent isolate (isolate No. 6) was tested for its virulence on ten tomato cultivars under greenhouse conditions. The cultivars were significantly varied in their susceptibility toward the tested virulent isolate. The most susceptible cultivar was Carmen F1cv as it recorded the highest value of infection (100% and 96%) in seeds and seedlings experiments, respectively. On the other hand the results showed that Diamond Arwa cultivar was the most resistant tested cultivar where zero infection percentage was recorded on both seeds and seedling experiments. The chemical analysis using HPLC was conducted with the most susceptible tomato cultivar (Carmen) and the most resistant one (Diamond Arwa) one and three weeks post inoculation with pathogenic isolate of Fusarium. The results revealed that the accumulation of total phenolic compounds either in shoots or roots was higher three weeks after inoculation than one week post inoculation within the two tested cultivars. Remarkable that roots and shoots of most resistant cultivar tested recorded more total phenol concentrations rather than the susceptible one. Significant differences were detected among the resistant and the susceptible cultivars tested with the compounds detected at 21.2m; 26,2m and 29.1 minute in both shoots and roots three weeks after pathogen inoculation which suggesting potential role of these particular chemicals in resistance mechanism toward Fusarium wilt disease within resistant tomato cultivars.","PeriodicalId":332125,"journal":{"name":"Menoufia Journal of Plant Protection","volume":"604 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124214767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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