Background: This research shows the improvement of student learning outcomes with Google Classroom-based learning on the concept of human reproductive systems in science classes. Methods: The research method used is the pre-experimental method one-group pretest-posttest design. Samples were taken in non-probability sampling with purposive sampling, which is a science class of 38 students. The treatment given in this study is learning done online using google classroom application, all activities in google classroom on the concept of the human reproductive system. Results: The results of the analysis showed with t calculate (-15.39) greater than t table (-2.04). Based on data analysis and testing, it can be interpreted that there is an increase in student learning outcomes with google classroom-based online learning. The increase is seen from the N-gain value (0.57), which interprets learning outcomes in medium theories. Conclusions: Effectivity learning activities need to be presented by teachers in the face of pandemic situations, although they cannot be done for luring. Google Classroom based online learning can package distance learning more effectively, which has an impact on improving student learning outcomes.
{"title":"Google Classroom-based Online Learning Study Outcomes on Reproductive Concept in Science Classes","authors":"Rosalia Dwi Putri, P. K. Suprapto, Egi Nuryadin","doi":"10.22236/j.bes/515241","DOIUrl":"https://doi.org/10.22236/j.bes/515241","url":null,"abstract":"Background: This research shows the improvement of student learning outcomes with Google Classroom-based learning on the concept of human reproductive systems in science classes. Methods: The research method used is the pre-experimental method one-group pretest-posttest design. Samples were taken in non-probability sampling with purposive sampling, which is a science class of 38 students. The treatment given in this study is learning done online using google classroom application, all activities in google classroom on the concept of the human reproductive system. Results: The results of the analysis showed with t calculate (-15.39) greater than t table (-2.04). Based on data analysis and testing, it can be interpreted that there is an increase in student learning outcomes with google classroom-based online learning. The increase is seen from the N-gain value (0.57), which interprets learning outcomes in medium theories. Conclusions: Effectivity learning activities need to be presented by teachers in the face of pandemic situations, although they cannot be done for luring. Google Classroom based online learning can package distance learning more effectively, which has an impact on improving student learning outcomes.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41420158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Vibriosis is currently still a major problem in marine aquaculture and is highly dependent on availability of antibacterial agents in its handling. Investigation of antibacterial potential of the coastal plants of Bidara Laut (Ximenia americana) has been carried out. Methods: Parts of the X. americana plant taken are Old Fruit, Young Fruit, Old Leaf, and Young Leaf. A total of ± 200 grams of each part of X. americana plant was taken to be extracted using maceration method. The antibacterial activity test was performed using the Kirby-Bauer disc diffusion method on Muller Hinton Agar (MHA) media. Observations were made in time units, namely every 6 hours of incubation for 24 hours. Results: The tested extract of plant parts of X. americana showed an inhibition zone against the growth of V. alginolyticus and V. parahaemolyticus bacteria in each replication in all-time units of observation. Of the four types of extracts tested, old leaves showed strong category resistance to V. alginolyticus with the highest clear zone diameter of 16 mm (average 14.67 mm) at 6 hours incubation. Young leaves gave the highest of 18 mm (average 16.33 mm) against V. parahaemolyticus at 6 hours incubation. Conclusions: X. americana plants have potential for antibacterial against Vibriosis disease in aquaculture. The ability of power to be seen tended to decrease for all tests until the end of observation, but until the end of observation, the ability of extract inhibition of all parts of the plant was still in the moderate to strong category.
{"title":"Antibacterial Potential of Bidara Laut (Ximenia americana) Plant Against Vibrio alginolyticus and V. parahaemolyticus Bacteria","authors":"Ramses, Fenny Agustina, R. P. P. Nugroho","doi":"10.22236/j.bes/515091","DOIUrl":"https://doi.org/10.22236/j.bes/515091","url":null,"abstract":"Background: Vibriosis is currently still a major problem in marine aquaculture and is highly dependent on availability of antibacterial agents in its handling. Investigation of antibacterial potential of the coastal plants of Bidara Laut (Ximenia americana) has been carried out. Methods: Parts of the X. americana plant taken are Old Fruit, Young Fruit, Old Leaf, and Young Leaf. A total of ± 200 grams of each part of X. americana plant was taken to be extracted using maceration method. The antibacterial activity test was performed using the Kirby-Bauer disc diffusion method on Muller Hinton Agar (MHA) media. Observations were made in time units, namely every 6 hours of incubation for 24 hours. Results: The tested extract of plant parts of X. americana showed an inhibition zone against the growth of V. alginolyticus and V. parahaemolyticus bacteria in each replication in all-time units of observation. Of the four types of extracts tested, old leaves showed strong category resistance to V. alginolyticus with the highest clear zone diameter of 16 mm (average 14.67 mm) at 6 hours incubation. Young leaves gave the highest of 18 mm (average 16.33 mm) against V. parahaemolyticus at 6 hours incubation. Conclusions: X. americana plants have potential for antibacterial against Vibriosis disease in aquaculture. The ability of power to be seen tended to decrease for all tests until the end of observation, but until the end of observation, the ability of extract inhibition of all parts of the plant was still in the moderate to strong category.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48124617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Gogo rice is a type of rice that can be cultivated on dry land with a yield productivity level of 2.57 tons / Ha. So it is necessary to make efforts to increase the growth and productivity of gogo rice through seed bio-inviguration using PGPR. The purpose of this study was to determine the effect of beni bioinviguration techniques using PGPR on the growth and productivity of gogo rice. Method: This study used a RAK consisting of 7 treatments and 5 replications. Data analysis used ANOVA at the 5% confidence level and continued with the BNT test. Result: The results showed that the use of biomatriconditioning medium integrated with PGPR could increase the stem height of upland rice by 104.2 cm, 117 cm, and 133.3, the number of tillers were 7, 13 and 15 at the age of 8, 10, and 12 MST. In addition, it can also increase the number of panicles, the length and weight of 100 grains of 14, 22.6 cm and 2.1 grams. Conclusion: the use of biomatriconditioning medium integrated with PGPR can increase the growth and productivity of gogo rice.
{"title":"Response of Seed Bioinvoguration with Plant Growth Promoting Rhizobacteria (PGPR) on Growth and Productivity of Rice gogo","authors":"F. Fitriani, Yulida Amri, S. Bahri, Fara Nadilla","doi":"10.22236/j.bes/515739","DOIUrl":"https://doi.org/10.22236/j.bes/515739","url":null,"abstract":"Background: Gogo rice is a type of rice that can be cultivated on dry land with a yield productivity level of 2.57 tons / Ha. So it is necessary to make efforts to increase the growth and productivity of gogo rice through seed bio-inviguration using PGPR. The purpose of this study was to determine the effect of beni bioinviguration techniques using PGPR on the growth and productivity of gogo rice. Method: This study used a RAK consisting of 7 treatments and 5 replications. Data analysis used ANOVA at the 5% confidence level and continued with the BNT test. Result: The results showed that the use of biomatriconditioning medium integrated with PGPR could increase the stem height of upland rice by 104.2 cm, 117 cm, and 133.3, the number of tillers were 7, 13 and 15 at the age of 8, 10, and 12 MST. In addition, it can also increase the number of panicles, the length and weight of 100 grains of 14, 22.6 cm and 2.1 grams. Conclusion: the use of biomatriconditioning medium integrated with PGPR can increase the growth and productivity of gogo rice.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44013611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The record and research on macrofungi from Taman Eden 100, Toba-Samosir Regency, North Sumatra, Indonesia, was conducted to provide some information about their current status and potential use in the future. Methods: This research was divided into three steps: exploration, identification, and literature studies of the potential use from the identified macrofungi. Results: The research had successfully identified 14 macrofungi that were classified into 4 orders and 9 families. All the macrofungi found were Basidiomycota, namely Gymnopilus sp., Marasmiellus sp.1, Marasmiellus sp.2, Marasmius sp., Favolaschia sp., Coprinellus sp., Coprinopsis sp., Auricularia sp.1, Auricularia sp.2, Auricularia sp.3, Auricularia sp.4, Tylopilus sp., Suillus sp., and Russula sp. The identified macrofungi are potentially used as food supplements, medicine, bio-fertilisers, bioherbicides, and bioremediation agents based on the literature. Conclusions: Taman Eden 100 has a unique fungal diversity of macrofungi and has never been published in a scientific journal. Further investigations are needed to determine the fungal diversity and potential use of macrofungi in other places in Taman Eden 100.
背景：对印度尼西亚北苏门答腊Toba Samosir Regency的Taman Eden 100大型真菌进行了记录和研究，以提供有关其现状和未来潜在用途的信息。方法：本研究分为三个步骤：探索、鉴定和文献研究已鉴定的大型真菌的潜在用途。结果：成功鉴定出14种大型真菌，分为4目9科。所有发现的大型真菌均为担子菌门，即Gymnopilus sp.、Marasmilus sp.1、Marasmelus sp.2、Marasmus sp.、Favolaschia sp.、Coprinellus sp、Coprinopsis sp.、Auricularia sp.1、Auricularia sp.2、Auricolaria sp.3、Auriclaria sp.4、Tylopilus sp，以及基于文献的生物修复剂。结论：Taman Eden 100具有独特的大型真菌多样性，从未在科学期刊上发表过。需要进一步的调查来确定真菌的多样性和大型真菌在塔曼伊甸园100其他地方的潜在用途。
{"title":"Notes Some Macro Fungi From Taman Eden 100, Kawasan Toba, Sumatera Utara, Indonesia: Description and Its Potency","authors":"O. D. Nurhayat, I. P. Putra, S. Anita, D. Yanto","doi":"10.22236/j.bes/515326","DOIUrl":"https://doi.org/10.22236/j.bes/515326","url":null,"abstract":"Background: The record and research on macrofungi from Taman Eden 100, Toba-Samosir Regency, North Sumatra, Indonesia, was conducted to provide some information about their current status and potential use in the future. Methods: This research was divided into three steps: exploration, identification, and literature studies of the potential use from the identified macrofungi. Results: The research had successfully identified 14 macrofungi that were classified into 4 orders and 9 families. All the macrofungi found were Basidiomycota, namely Gymnopilus sp., Marasmiellus sp.1, Marasmiellus sp.2, Marasmius sp., Favolaschia sp., Coprinellus sp., Coprinopsis sp., Auricularia sp.1, Auricularia sp.2, Auricularia sp.3, Auricularia sp.4, Tylopilus sp., Suillus sp., and Russula sp. The identified macrofungi are potentially used as food supplements, medicine, bio-fertilisers, bioherbicides, and bioremediation agents based on the literature. Conclusions: Taman Eden 100 has a unique fungal diversity of macrofungi and has never been published in a scientific journal. Further investigations are needed to determine the fungal diversity and potential use of macrofungi in other places in Taman Eden 100.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45134412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This study aims to develop and produce a diagnostic tool capable of detecting student misconceptions on the concept of ecosystems. Methods: This study used a research and development method with a modified 4D design (Define, Design, Develop and Disseminate) without disseminating or spreading. Data collection methods used in this research and development are observation, literature review, documentation, questionnaires, interviews and tests. Results: The results showed that the instruments developed were valid by validating 3 experts, namely material experts, evaluation and assessment experts, and linguists with an average percentage of each feasibility of 90% material expert judgment, 82% assessment expert assessment and evaluation. and 80% linguist assessment. Meanwhile, the instrument readability test showed a percentage of 63% in good category. The validity value of the instrument in general was 0.70 with the valid or high validity category, while the instrument reliability value was 0.82 with the very high category of reliability. The overall results of students' misconceptions on ecosystem material showed 23% of students experienced low-category miconceptions, 8% of students experienced no conceptual understanding, 17% of students experienced conceptual understanding, and 52% of students experienced partial understanding. Conclusion: Based on these results it can be said that the four-tier test instrument developed is feasible and can diagnose misconceptions in students
{"title":"Four Tier Test Ecosystem: Diagnostic Test to Detect Misconception on Ecosystem Concepts","authors":"Lintang, P. K. Suprapto, R. Ardiansyah","doi":"10.22236/j.bes/515750","DOIUrl":"https://doi.org/10.22236/j.bes/515750","url":null,"abstract":"Background: This study aims to develop and produce a diagnostic tool capable of detecting student misconceptions on the concept of ecosystems. Methods: This study used a research and development method with a modified 4D design (Define, Design, Develop and Disseminate) without disseminating or spreading. Data collection methods used in this research and development are observation, literature review, documentation, questionnaires, interviews and tests. Results: The results showed that the instruments developed were valid by validating 3 experts, namely material experts, evaluation and assessment experts, and linguists with an average percentage of each feasibility of 90% material expert judgment, 82% assessment expert assessment and evaluation. and 80% linguist assessment. Meanwhile, the instrument readability test showed a percentage of 63% in good category. The validity value of the instrument in general was 0.70 with the valid or high validity category, while the instrument reliability value was 0.82 with the very high category of reliability. The overall results of students' misconceptions on ecosystem material showed 23% of students experienced low-category miconceptions, 8% of students experienced no conceptual understanding, 17% of students experienced conceptual understanding, and 52% of students experienced partial understanding. Conclusion: Based on these results it can be said that the four-tier test instrument developed is feasible and can diagnose misconceptions in students","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43156756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Bacteria are unicellular prokaryotic microorganisms which are abundant in nature. This study aims to isolate and identify bacteria present in the leaves of Kawista (Limonia acidissima L.). Method: The identification process was carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. The study was conducted at the West Nusa Tenggara Province Testing and Calibration Laboratory for Health, using kawista (Limonia acidissima L.) leaves obtained from West Sumbawa Regency. Results: The results showed eight bacterial isolates were found including Bacillus sp, Bacillus megaterium, Staphylococcus sp, Staphylococcus epidermidis, Staphylococcus capitis, Streptococcus oralis, Leuconostoc mesenteroides spp. mesenteroides, and Shewanella putrefaciens. Conclusion: Diversity of types of bacteria can be studied through the process of identification. This study aims to isolate and identify bacteria found in kawista leaves. The identification method is carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. Bacteria found on the leaves of kawista (Limonia acidissima L.) consist of a group of endophytic bacteria, lactic acid bacteria, metal reducing bacteria, and pathogenic bacteria.
{"title":"Identification of Bacteria in Kawista Leaves (Limonia acidissima L.)","authors":"J. Aulia, Nining Purwati, S. S. Umami","doi":"10.22236/j.bes/515568","DOIUrl":"https://doi.org/10.22236/j.bes/515568","url":null,"abstract":"Background: Bacteria are unicellular prokaryotic microorganisms which are abundant in nature. This study aims to isolate and identify bacteria present in the leaves of Kawista (Limonia acidissima L.). Method: The identification process was carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. The study was conducted at the West Nusa Tenggara Province Testing and Calibration Laboratory for Health, using kawista (Limonia acidissima L.) leaves obtained from West Sumbawa Regency. Results: The results showed eight bacterial isolates were found including Bacillus sp, Bacillus megaterium, Staphylococcus sp, Staphylococcus epidermidis, Staphylococcus capitis, Streptococcus oralis, Leuconostoc mesenteroides spp. mesenteroides, and Shewanella putrefaciens. Conclusion: Diversity of types of bacteria can be studied through the process of identification. This study aims to isolate and identify bacteria found in kawista leaves. The identification method is carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. Bacteria found on the leaves of kawista (Limonia acidissima L.) consist of a group of endophytic bacteria, lactic acid bacteria, metal reducing bacteria, and pathogenic bacteria.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41398669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahsanul Buduri Agustiar, Dewi Masyitoh, Irda Dwi Fibriana, Adesilvi Saisatul Khumairoh, Kurnia Alfi Rianti, Norma Fitriani, Muhammad Harissuddin, H. A. Akmalia
Background: Biodiversity in Indonesia is so diverse, including in Apocynaceae plants that is why it is important to study the kinship relationship to find out the kinship of Apocynaceae. The purpose of this study was to determine phenetic kinship through morphological and anatomical evidence from four members of the Apocynaceae family. Methods: The method used in this research is descriptive qualitative and quantitative method. The samples in this study were four species of Apocynaceae family members, including Adenium obesum, Plumeria rubra, Catharanthus roseus, and Allamanda cathartica. The indicators used were the morphological traits of stems, leaves, and flowers and the anatomical trait of stomata. Results: The result showed that the phenetic kinship of the four species of the Apocynaceae family member namely Alamanda cathartica had a distant kinship relationship with the other species with a similarity value of 31%. Conclusions: Thus, the familial relationship between species in the Apocynaceae family in terms of morphological and anatomical characters that have a close relationship with Plumeria rubra and Adenium obesum with a similarity value of 44% and the most distant Alamanda cathartica with a similarity value of 31%.
{"title":"Phenetic Kinship Relationship of Apocynaceae Family Based on Morphological and Anatomical Characters","authors":"Ahsanul Buduri Agustiar, Dewi Masyitoh, Irda Dwi Fibriana, Adesilvi Saisatul Khumairoh, Kurnia Alfi Rianti, Norma Fitriani, Muhammad Harissuddin, H. A. Akmalia","doi":"10.22236/j.bes/424945","DOIUrl":"https://doi.org/10.22236/j.bes/424945","url":null,"abstract":"Background: Biodiversity in Indonesia is so diverse, including in Apocynaceae plants that is why it is important to study the kinship relationship to find out the kinship of Apocynaceae. The purpose of this study was to determine phenetic kinship through morphological and anatomical evidence from four members of the Apocynaceae family. Methods: The method used in this research is descriptive qualitative and quantitative method. The samples in this study were four species of Apocynaceae family members, including Adenium obesum, Plumeria rubra, Catharanthus roseus, and Allamanda cathartica. The indicators used were the morphological traits of stems, leaves, and flowers and the anatomical trait of stomata. Results: The result showed that the phenetic kinship of the four species of the Apocynaceae family member namely Alamanda cathartica had a distant kinship relationship with the other species with a similarity value of 31%. Conclusions: Thus, the familial relationship between species in the Apocynaceae family in terms of morphological and anatomical characters that have a close relationship with Plumeria rubra and Adenium obesum with a similarity value of 44% and the most distant Alamanda cathartica with a similarity value of 31%. \u0000 \u0000 ","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47012330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Diabetes Mellitus is a chronic metabolic disorder caused by insulin resistance. Noni juice has the potential as herbal medicine which believed to reduce blood glucose levels. This study aims to determine the effect of noni juice on blood sugar levels on diabetic patients. Methods: This study used 16 respondents. They divided two groups, a control group and a treatment group. Each group consists of 8 respondents. The intervention group received 150 ml of noni juice which was given once a day for ten days. Glucose testing was carried out by the glucose-check method. The blood was taken from the respondent's fingertips capillary. The blood glucose levels between the control group and the intervention group were analyzed using independent t-test. Results: The average blood glucose level in the intervention group was 199.88 mg/dl, while the control group was 326.25 mg/dl. The test results of the mean blood glucose levels between the control group and the intervention group had a significant difference with a value of p = 0.003 (p ≤ 0.05). This indicates a significant effect between blood glucose levels in the control group and the intervention group. Conclusion: Noni can reduce blood glucose levels because it contains flavonoid. It has a hypoglycemic effect. Health workers can suggest consuming noni juice to decrease blood glucose in diabetic patients.
{"title":"The Potential of Noni (Moringa citrifolia) in Lowering Blood Glucose Levels in Diabetes Mellitus Patients","authors":"Putri Dafriani, Mutiara Karamika, Siska Sakti Anggraini, Roza Marlinda","doi":"10.22236/j.bes/424919","DOIUrl":"https://doi.org/10.22236/j.bes/424919","url":null,"abstract":"Background: Diabetes Mellitus is a chronic metabolic disorder caused by insulin resistance. Noni juice has the potential as herbal medicine which believed to reduce blood glucose levels. This study aims to determine the effect of noni juice on blood sugar levels on diabetic patients. Methods: This study used 16 respondents. They divided two groups, a control group and a treatment group. Each group consists of 8 respondents. The intervention group received 150 ml of noni juice which was given once a day for ten days. Glucose testing was carried out by the glucose-check method. The blood was taken from the respondent's fingertips capillary. The blood glucose levels between the control group and the intervention group were analyzed using independent t-test. Results: The average blood glucose level in the intervention group was 199.88 mg/dl, while the control group was 326.25 mg/dl. The test results of the mean blood glucose levels between the control group and the intervention group had a significant difference with a value of p = 0.003 (p ≤ 0.05). This indicates a significant effect between blood glucose levels in the control group and the intervention group. Conclusion: Noni can reduce blood glucose levels because it contains flavonoid. It has a hypoglycemic effect. Health workers can suggest consuming noni juice to decrease blood glucose in diabetic patients. \u0000 \u0000 ","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42542780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Hypertension is a disease with increasing characteristics of blood pressure. The ACE gene has a role in the conversion of ATI to ATII in hypertensive conditions. Healing is done by using the 8-gingerol content contained in ginger. The purpose of this study is to analyze the molecular interaction that occurs between 8-gingerol and ACE. Method: ACE model proteins (ID: 3bkk) were obtained from the Bank Data Protein database (PDB) through 8-gingerol ligands (CID: 168114) obtained from the PubChem database. ACE and 8-gingerol were docked by Discovery Study Client 4.1 software. Analysis of amino acid residues, binding energy, Van der Waals forces, and hydrogen bonds formed using Discovery Studio Client 4.1. Results: The interaction between 8-gingerol and ACE showed that there were seven amino acid residues that interacted with 8-gingerol, also found hydrogen bonds, hydrophobic and Van der Waals forces that strengthen and stabilize these bonds. Conclusion: the interaction of 8-ginger with the active side of ACE is determined as an ACE inhibitor, the inhibition is a significant effect on the obstruction of ACE conversion.
背景:高血压是一种以血压升高为特征的疾病。ACE基因在高血压患者ATI向ATII的转化中起作用。治疗是通过使用生姜中含有的8-姜辣素来完成的。本研究的目的是分析8-姜辣素与ACE之间的分子相互作用。方法:通过从PubChem数据库中获取8-姜辣素配体(CID: 168114)，从Bank Data Protein database (PDB)中获得ACE模型蛋白(ID: 3bkk)。ACE和8-姜辣素通过Discovery Study Client 4.1软件对接。使用Discovery Studio Client 4.1分析氨基酸残基、结合能、范德华力和氢键。结果:8-姜辣素与ACE的相互作用表明，有7个氨基酸残基与8-姜辣素相互作用，并发现氢键、疏水性和范德华力加强和稳定了这些键。结论:8-姜与ACE活性侧相互作用确定为ACE抑制剂，对ACE转化受阻有明显抑制作用。
{"title":"Analysis of molecular interactions of 8-gingerol compounds in Ginger (Zingiber officinale) as ACE Inhibitor","authors":"Y. Bare, M. S, Aprianus Pani Pili, Maria Helvina","doi":"10.22236/j.bes/424944","DOIUrl":"https://doi.org/10.22236/j.bes/424944","url":null,"abstract":"Background: Hypertension is a disease with increasing characteristics of blood pressure. The ACE gene has a role in the conversion of ATI to ATII in hypertensive conditions. Healing is done by using the 8-gingerol content contained in ginger. The purpose of this study is to analyze the molecular interaction that occurs between 8-gingerol and ACE. Method: ACE model proteins (ID: 3bkk) were obtained from the Bank Data Protein database (PDB) through 8-gingerol ligands (CID: 168114) obtained from the PubChem database. ACE and 8-gingerol were docked by Discovery Study Client 4.1 software. Analysis of amino acid residues, binding energy, Van der Waals forces, and hydrogen bonds formed using Discovery Studio Client 4.1. Results: The interaction between 8-gingerol and ACE showed that there were seven amino acid residues that interacted with 8-gingerol, also found hydrogen bonds, hydrophobic and Van der Waals forces that strengthen and stabilize these bonds. Conclusion: the interaction of 8-ginger with the active side of ACE is determined as an ACE inhibitor, the inhibition is a significant effect on the obstruction of ACE conversion.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48750092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.
{"title":"Antimicrobial Activity and Identification of Gene Encoding Enterocin Enterococcus faecalis K2B1 Isolated from Toraja's Belang Buffalo Milk","authors":"H. Alang, J. Kusnadi, T. Ardyati, Suharjono","doi":"10.22236/j.bes/425193","DOIUrl":"https://doi.org/10.22236/j.bes/425193","url":null,"abstract":"Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative. \u0000 \u0000 ","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44263100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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