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EFEKTIVITAS PENYUNTIKAN FSH SECARA SUBKUTAN DAN INTRAMUSKULAR TERHADAP RESPON SUPEROVULASI SAPI SIMENTAL FSH皮下注射和肌肉注射对奶牛排卵反应的影响
Pub Date : 2019-01-09 DOI: 10.30997/JPNU.V4I1.1512
M. Djunaedi, R. Handarini, Deasy Zamanti
Superovulation is a necessary technique to produce large number of embryos for embryo` transfer. Hormonal treatment is superovulation methode can be done by implant CIDR and injection of Follicle Stimulating Hormone (FSH). Experiment were carried out to observe the effectiveness of subcutaneous and intramuscular FSH injections on superovulation response in simmental cattle. All animal (n=10) were treated with intravaginal CIDR implant before FSH injection. Studies were devided into two experiment ie: P1 (400 mg FSH diluted in 4 ml sterile diluent) injected in five simmental cattle by single subcutan injection and P2 (400 mg FSH diluted in 20 ml sterile diluent) injected in five simmental cattle by twice daily intramuscular injection over 4 day in decreasing doses. The number of corposa luteal (CL), total embryos collected, and total transferable embryos were observed in this experiment. Data were analyzed by T-test method. The result showed that effectiveness of single subcutan FSH injection were significanthy different (P < 0,05) than intramuscular FSH injection superovulation with single subcutan FSH injetcion is easier than twice daily intramuscular injection in decreasy dose. In conclusion the average of CL (21,4 ± 3,6) and number of tranferable embryo (71,96 %) of the single subcutan FSH injection tended to be better than intramuscular FSH injection. Single subcutan FSH injection more efficient than intramuscular FSH injection. Single subcutan injection can decreasing sterss level in the cattle and be easier in handling the cattle during the experiment.Keywords: Simmental cattle, FSH, superovulation, single subcutan injection, intramuscular injection.
超排卵是产生大量胚胎进行胚胎移植的必要技术。激素治疗为超排卵法,可通过植入CIDR和注射促卵泡激素(FSH)进行治疗。本试验旨在观察皮下注射和肌肉注射促卵泡刺激素对西门特牛超排卵反应的影响。所有动物(n=10)在注射FSH前经阴道内CIDR植入治疗。研究分为两个实验,即P1 (400 mg FSH,用4 ml无菌稀释液稀释)单次皮下注射5头西门门牛,P2 (400 mg FSH,用20 ml无菌稀释液稀释)每天2次肌肉注射,连续4天,剂量递减。观察黄体体(CL)数量、收集胚胎总数和可移植胚胎总数。数据采用t检验方法进行分析。结果表明,单次皮下注射促卵泡刺激素与肌肉注射促卵泡刺激素的效果差异有统计学意义(P < 0.05),单次皮下注射促卵泡刺激素比肌肉注射促卵泡刺激素在减少剂量时更容易产生超排卵。结果表明,单次皮下注射促卵泡刺激素的平均卵泡细胞数(21,4±3,6)和可移植胚胎数(71,96%)均优于肌肉注射促卵泡刺激素。单次皮下注射比肌内注射更有效。单次皮下注射可以降低牛的应激水平,并且在试验过程中更容易处理牛。关键词:西门特牛,卵泡刺激素,超排卵,单次皮下注射,肌肉注射
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引用次数: 0
EFFECTS OF SUBSTITUTION OF BASAL FEED WITH FERMENTED NON CONVENTIONAL FEED IN RATION ON PHYSICAL QUALITY OF BROILER MEAT 发酵非常规饲料替代日粮基础饲料对肉鸡肉品质的影响
Pub Date : 2018-10-30 DOI: 10.30997/JPNU.V4I2.1541
Sandra Dwi Pangestika, Elis Dihansih, A. Anggraeni
Demand for broiler meat for fulfilling people’s need for animal protein is increasing as it is nutritious and cheap in price. This study was aimed at assessing the effects of substitution of basal feed with fermented non conventional feed in ration on physical quality of broiler meat. The study was conducted from May to June 2017 at the Animal Laboratory of Djuanda University, Bogor. Proximate analysis on palm kernel cake, coconut cake, tofu waste product, and dried cassava was conducted at the laboratory of the Research Center of Biological Resources and Biotechnology, Bogor Agricultural University. Twenty-four of broiler chickens were used. A completely randomized design with 6 treatments and 4 replicates was used. Treatments consisted of 100% basal ration (R0, control), 90% basal ration + 10% non conventional feed (R1), 75% basal ration + 25% non conventional feed (R2), 60% basal ration + 40% non conventional feed (R3), 45% basal ration + 55% non conventional feed (R4), and 30% basal ration + 70% non conventional feed (R5). Results showed that water holding capacity, water content, and tenderness of meat were significantly different (P<0.05) but pH and cooking loss were not (P>0.05). Key words: broiler chicken, non conventional feed, meat physical quality
由于肉鸡营养丰富,价格便宜,满足人们对动物蛋白的需求,肉鸡的需求正在增加。本试验旨在评价在日粮中以发酵非常规饲料替代基础饲料对肉鸡肉品质的影响。该研究于2017年5月至6月在茂物大安达大学动物实验室进行。在茂物农业大学生物资源与生物技术研究中心实验室对棕榈仁饼、椰子饼、豆腐废料和干木薯进行了近似分析。选用24只肉鸡。试验采用完全随机设计,6个处理,4个重复。对照组为100%基础日粮(R0)、90%基础日粮+ 10%非常规饲料(R1)、75%基础日粮+ 25%非常规饲料(R2)、60%基础日粮+ 40%非常规饲料(R3)、45%基础日粮+ 55%非常规饲料(R4)和30%基础日粮+ 70%非常规饲料(R5)。结果表明,不同处理的肉的保水能力、含水量和嫩度均有显著差异(P0.05)。关键词:肉鸡,非常规饲料,肉质物理品质
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Jurnal Peternakan Nusantara
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