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Alternative procedures for parent choice in a breeding program for the common bean (Phaseolus vulgaris L.) 菜豆(Phaseolus vulgaris L.)育种中亲本选择的备选程序
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400014
L. B. Oliveira, M. Ramalho, A. F. B. Abreu, D. F. Ferreira
Six common bean cultivars were crossed in diallel and the segregant populations were assessed in the F2 and F3 generations to compare methodologies for parental selection in a breeding program based on hybridization. The cultivars involved in the diallel were A 114, A 77, ESAL 686, Milionario, Carioca, and Flor de Mayo. The segregant F2 and F3 generations were assessed on the experimental campus of the Universidade Federal de Larvas, in July 1994. It was found that the cultivars differed in their general combining ability (GCA). Flor de Mayo, which belongs to the Durango race, had the largest positive GCA estimate for grain field, and the cultivars from the Mesoamerican race, Milionario and A 114, the smallest GCA estimates. For flowering, the cultivar that most contributed to reduced plant cycle was ESAL 686. There was agreement among the results obtained from the diallel and the estimates of the parameter m + a of the populations. However, it was evident that the estimate of genetic variance of the populations should be considered as a condition to identify the hybrid population that will produce a line with high performance.
采用双列杂交方法对6个普通豆品种进行了F2和F3代分离群体的鉴定,比较了杂交育种中亲本选择的方法。参与双列杂交的品种有a114、a77、ESAL 686、Milionario、Carioca和Flor de Mayo。1994年7月在Larvas联邦大学的实验校园对F2和F3代种族进行了评估。结果表明,不同品种的一般配合力(GCA)存在差异。杜兰戈(Durango)品种的Flor de Mayo的谷地GCA值最大,中美洲品种Milionario和a114的GCA值最小。在开花方面,对缩短植株周期贡献最大的品种是ESAL 686。双列杂交所得结果与种群参数m + a的估计值一致。然而,很明显,群体遗传方差的估计应该作为鉴定杂种群体产生高性能品系的条件。
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引用次数: 13
Transferrin polymorphism in amazon turtle (podocnemis expansa) stocks 亚马逊龟种群转铁蛋白多态性研究
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400004
A. S. Teixeira, A. Jamieson, J. Raposo, Alvaro Alves Vieira
The transferrin gene locus (Tf) was investigated in five populations of the Amazon turtle (Podocnemis expansa) sampled from five geographical areas in the Amazon region. This locus was polymorphic, showing three genotypes (Tfa Tfa, Tfa Tfb and Tfb Tfb), presumably encoded by two co-dominant alleles, Tfa and Tfb. All populations showed good genetic balance according to Hardy-Weinberg expectations, and may sustain the hypothesis of a single stock in the area investigated. The data are consistent with free flow of genes among the population samples examined.
对亚马逊地区5个地理区域的亚马逊龟(Podocnemis expansa) 5个种群的转铁蛋白基因座(Tf)进行了研究。该位点具有多态性,表现出三种基因型(Tfa Tfa、Tfa Tfb和Tfb Tfb),可能由Tfa和Tfb两个共显性等位基因编码。根据Hardy-Weinberg的预期,所有种群都表现出良好的遗传平衡,并可能支持调查地区单一种群的假设。这些数据与基因在被检查的种群样本中自由流动的情况一致。
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引用次数: 5
Deletion/inversion in the X-chromosome and increased telomeric associations in a female with primary amenorrhea 原发性闭经女性患者x染色体缺失/反转和端粒关联增加
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551997000300025
A. Multani, V. C. Shah, Divya Singh, Nivedita Chakravarty, N. J. Chinoy, S. Pathak
We describe a new case of a partial interstitial deletion and inversion of the long arm of the X-chromosome associated with a high incidence of telomeric associations in an 18-year old female who showed underdeveloped secondary sex characteristics, including small breasts and primary amenorrhea. Her karyotype was considered to be 46,X,del(Xq13 ® q22)inv(X)(q23-q27). The buccal mucosal cells showed absence of a typical Barr body, and the 5’-bromo-2-deoxyuridine incorporation studies revealed that neither the normal X-nor the abnormal X-chromosome was late replicating. The case is being presented for its extreme rarity
我们描述了一个新的病例,部分间质缺失和倒位的x染色体长臂相关的端粒关联高发的18岁女性谁表现出不发达的第二性特征,包括小乳房和原发性闭经。推测其核型为46,X,del(Xq13®q22)inv(X)(q23-q27)。颊粘膜细胞缺乏典型的Barr小体,5′-溴-2-脱氧尿苷结合研究显示正常x染色体和异常x染色体均未发生晚期复制。这一案件因其极其罕见而被提起
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引用次数: 0
A simple model for the estimation of congenital malformation frequency in racially mixed populations 一个估计种族混合人群先天性畸形发生率的简单模型
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400024
J. López-Camelo, P. Cabello, M. G. Dutra
A simple model is proposed, using the method of maximum likelihood to estimate malformation frequencies in racial groups based on data obtained from hospital services. This model uses the proportions of racial admixture, and the observed malformation frequency. It was applied to two defects: postaxial polydactyly and cleft lip, the frequencies of which are recognizedly heterogeneous among racial groups. The frequencies estimated in each racial group were those expected for these malformations, which proves the applicability of the method.
提出了一个简单的模型,使用最大似然法估计畸形频率的种族群体基于从医院服务获得的数据。该模型采用种族混合比例和观察到的畸形频率。它被应用于两种缺陷:轴后多指畸形和唇裂,其频率在种族群体中是公认的异质。在每个种族组中估计的频率与这些畸形的预期频率一致,证明了该方法的适用性。
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引用次数: 28
Holstein white coat color and performance: phenotypic, genetic and environmental correlations 荷斯坦白被毛颜色与性能:表型、遗传和环境相关性
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400009
C. M. Becerril, C. J. Wilcox, V. Guerrero
Correlations of measures of percentages of white coat color, five measures of production and two measures of reproduction were obtained from 4293 first lactation Holsteins from eight Florida dairy farms. Percentages of white coat color were analyzed as recorded and transformed by an extension of Box-Cox procedures. Statistical analyses were by derivative-free restricted maximum likelihood (DFREML) with an animal model. Phenotypic and genetic correlations of white percentage (not transformed) were with milk yield, 0.047 and 0.097; fat yield, 0.002 and 0.004; fat percentage, -0.047 and -0.090; protein yield, 0.024 and 0.048; protein percentage, -0.070 and -0.116; days open, -0.012 and -0.065; and calving interval, -0.007 and -0.029. Changes in magnitude of correlations were very small for all variables except days open. Genetic and phenotypic correlations of transformed values with days open were -0.027 and -0.140. Modest positive correlated responses would be expected for white coat color percentage following direct selection for milk, fat, and protein yields, but selection for fat and protein percentages, days open, or calving interval would lead to small decreases.
从佛罗里达州8个奶牛场的4293头哺乳期荷斯坦奶牛中获得了白色毛色百分比、5个产量指标和2个繁殖指标的相关性。白色被毛的百分比被记录下来,并通过Box-Cox程序的扩展进行转换。统计分析采用无导数限制最大似然(DFREML)与动物模型。白百分率(未转化)与产奶量的表型和遗传相关分别为0.047和0.097;脂肪产量分别为0.002和0.004;脂肪率,-0.047和-0.090;蛋白产量分别为0.024和0.048;蛋白质百分比,-0.070和-0.116;开盘日,-0.012和-0.065;产犊间隔为-0.007和-0.029。除开放日外,所有变量的相关性变化幅度都很小。转化值与开放天数的遗传相关和表型相关分别为-0.027和-0.140。在直接选择产奶量、脂肪量和蛋白质产量后,预期白毛色百分比会有适度的正相关反应,但选择脂肪和蛋白质百分比、开日数或产犊间隔会导致小幅下降。
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引用次数: 5
Karyotype of a termitophilic species of Diplopoda (Polydesmida, Chelodesmidae) 双足动物一种嗜白蚁物种的核型研究(多翅目,切翅目)
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400010
C. S. Fontanetti
This report describes the karyotype of a Brazilian Diplopod species (Arthropoda), Sandalodesmus gasparae (Schubart, Acta Zool. Lilloana Inst. "Miguel Lillo" 2: 321-440,1944), collected from a termite mound Cornitermes bequaerti. This species has 2n = 12. The sex pair could not be distinguished.
本文报道了巴西二足动物(节肢动物)Sandalodesmus gasparae (Schubart, Acta Zool)的核型。Lilloana本月。《米格尔·利洛》2:321-440,1944),采集自白蚁丘Cornitermes bequaerti。这个物种有2n = 12。性别对无法区分。
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引用次数: 8
Cytogenetics of six Brazilian species of Psocoptera 巴西六种棘翅目昆虫的细胞遗传学
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400011
E. Zefa, C. S. Fontanetti, A. Mesa
The karyotypes of the following six species of Brazilian Psocoptera are reported: Caecillius sp. (Caecillidae), Triplocania ? caudata New (Ptiloneuridae), Brachinodiscus cf. lepidus (Banks) (Psocidae), Psococerastis interrupta New (Psocidae), Ptycta nr reticulata New (Psocidae) and Trichadenotecnum sinuatum New (Psocidae). All of them had males with 2n = 17 and an XO sex determining mechanism.
报告了以下 6 种巴西鞘翅目昆虫的核型:Caecillius sp. (Caecillidae), Triplocania ? caudata New (Ptiloneuridae), Brachinodiscus cf. lepidus (Banks) (Psocidae), Psocerastis interrupta New (Psocidae), Ptycta nr reticulata New (Psocidae) and Trichadenotecnum sinuatum New (Psocidae).它们的雄性个体均为 2n = 17,性别决定机制为 XO。
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引用次数: 4
Copper accumulation in Xanthomonas campestris pv. vesicatoria 油菜黄单胞菌中铜的积累。实验
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400002
G. Ramos, Y. B. Rosato
A strain of Xanthomonas campestris pv. vesicatoria showing resistance to 1.2 mM cupric sulfate was analyzed by atomic absorption spectroscopy and ESI (electron spectrophotometry imaging). Accumulation of copper was detected in the periphery of the cell membrane region, suggesting that the mechanism of copper resistance is similar to that previously described for Pseudomonas species. The ESI technique was used to detect copper in the membrane region. Copper-resistance in X. campestris pv. vesicatoria 484 is inducible and occurs by accumulation of the metal and not by efflux mechanism as has been suggested. The growth curve also showed that this system is inducible.
一株油菜黄单胞菌。采用原子吸收光谱和ESI(电子分光光度法)分析了对1.2 mM硫酸铜的抗性。在细胞膜外围区域检测到铜的积累,这表明铜抗性的机制与之前描述的假单胞菌种类相似。采用ESI技术对膜区铜进行了检测。田鼠的耐铜性。蒸发量484是可诱导的,并通过金属的积累而不是通过外排机制发生。生长曲线也表明该体系是可诱导的。
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引用次数: 2
The polytene chromosomes of the mosquito Anopheles bellator compared with those of Anopheles cruzii 贝拉按蚊与克氏按蚊多染色体的比较
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400003
C. Ramirez, E. Dessen
A photographic map was made of polytene chromosomes of ovarian nurse cells of Anopheles bellator females. The chromosomes of this species have complete or partial homology with those of A. cruzii, mainly in the telomeric and centromeric regions. Variability at the single band level was observed as asymmetric bands at seven different positions. One inversion (3Ra) was detected in the 3R arm.
对雌性贝拉按蚊卵巢护理细胞的多烯染色体进行了照相图谱。本种的染色体主要在端粒区和着丝粒区具有完全或部分同源性。在单波段水平上,在7个不同位置观察到不对称波段。在3R臂检测到1个反转(3Ra)。
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引用次数: 8
Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells AluI和BamHI诱导中国仓鼠卵巢细胞染色单体间隙和断裂的显微扫描
Pub Date : 1900-01-01 DOI: 10.1590/S0100-84551996000400007
W. Martínez-López, R. Bonomi, G. Folle, M. Drets
Chromatid gaps and breaks induced by the restriction endonucleases AluI and BamHI in the long arm of chromosome 1 of Chinese hamster ovary cells were microphotometrically scanned and mapped to a quantitative G-band map. More than 50% of chromatid breaks appeared as chromatin losses of greater than 5% of the total arm length. The majority of chromatid gaps and breaks as well as chromatin losses induced by both restriction endonucleases were non-randomly located in a region from 0.35 to 0.65 relative length units of the long arm of chromosome 1. We suggest that the access of these endonucleases to chromosomal DNA depends on the local organization of the chromatin.
对中国仓鼠卵巢细胞1号染色体长臂上限制性内切酶AluI和BamHI诱导的染色单体间隙和断裂进行了显微扫描,并绘制了定量的g波段图。超过50%的染色单体断裂表现为染色质损失大于总臂长的5%。由这两种限制性内切酶引起的染色单体间隙和断裂以及染色质损失的大部分非随机分布在1号染色体长臂0.35 ~ 0.65相对长度单位的区域。我们认为这些内切酶进入染色体DNA取决于染色质的局部组织。
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引用次数: 4
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Brazilian Journal of Genetics
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