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Establishing phosphorus critical values for tomato (Solanum lycopersicum) fertilization with phosphate fertilizers on the Sudan savanna soils using three soil phosphorus extraction methods and field experimentation in Kano State, Nigeria 利用三种土壤磷提取方法和尼日利亚卡诺州的田间试验,在苏丹稀树草原土壤上建立番茄(番茄)施用磷肥的磷临界值
Q2 Agricultural and Biological Sciences Pub Date : 2023-08-07 DOI: 10.3389/fagro.2023.1181045
Yahaya Mohammad Yusuf, D. K. Madukwe, F. Kebede
Phosphorus (P) is an essential nutrient for tomato (Solanum lycopersicum L.) production, as it stimulates root growth by improving water use efficiency and nutrient uptake. It also helps control the metabolic processes of ATP (energy), sugars, and nucleic acids. Therefore, it is imperative to effectively manage the phosphorus requirements of tomatoes to bolster their productivity. The objective of this study was to evaluate the effectiveness of three different soil P testing methods that are suitable for the soils of Kano State, Nigeria, and to develop a critical P-value for fertilizer recommendations for tomatoes. Accordingly, sixty topsoil samples were collected for soil available P testing according to the Bray II, Mehlich 3, and Olsen methods. Moreover, to establish the phosphorus critical value (Pc) for tomatoes, a field experiment was conducted with seven different rates of P using triple super phosphate fertilizer (TSP), viz., 0, 15, 30, 45, 60, 75, and 90 kg P/ha, laid out in a randomized complete block design with three replications on 15 experimental sites representing the various agroecological zones of the state. Phosphorus fertilizer was applied and incorporated into the soil 1 week before tomato transplanting. Then, soil samples from 0 to 20 cm depth were taken 3 weeks after transplanting from all the plots across the experimental sites for separate Mehlich 3, Bray II, and Olsen extractable P analysis to determine the critical soil P (Pc) test. The study revealed that available P was varied and increased in the order of Olsen (2.19 ppm)< Mehlich 3 (5.37 ppm)< Bray II (5.44 ppm), and the Mehlich 3 and Bray II extraction methods were strongly correlated, whereas Olsen underestimated the available P, showing a weak correlation with both Mehlich 3 and Bray II. Furthermore, the study showed that phosphorus critical (Pc) values were 17.00, 14.00, and 4 ppm for Mehlich 3, Bray II, and Olsen respectively. Finally, Bray II was a more reliable method for estimating the available P on moderately acid soils as it showed the highest correlation r = 0.83 with the relative yield of the tomatoes and, therefore, was the most effective method for developing the equation for the soil test-based phosphatic fertilizer recommendation for maximizing tomato production in the soils of Kano State. Hence, the equation developed for the P fertilizer recommendation (Pr) for tomatoes in Kano State was found to be “Pr (kg P2O5 ha−1) = (14.0 − Pi) × 9.02”.
磷(P)是番茄(Solanum lycopersicum L.)生产的必需营养素,因为它通过提高水分利用效率和养分吸收来刺激根系生长。它还有助于控制ATP(能量)、糖和核酸的代谢过程。因此,必须有效管理番茄的磷需求,以提高其生产力。本研究的目的是评估适用于尼日利亚卡诺州土壤的三种不同土壤磷测试方法的有效性,并制定番茄肥料推荐的临界磷值。因此,根据Bray II、Mehlich 3和Olsen方法,收集了60个表层土样本用于土壤有效磷测试。此外,为了确定番茄的磷临界值(Pc),在代表该州不同农业生态区的15个试验点上进行了三重超磷酸盐(TSP)田间试验,用7种不同的磷率,即0、15、30、45、60、75和90 kg P/ha,以随机完全区组设计进行了三次重复。在番茄移植前1周施用磷肥并将其掺入土壤中。然后,在移植后3周,从实验地点的所有地块中采集0至20cm深度的土壤样本,分别进行Mehlich 3、Bray II和Olsen可提取磷分析,以确定临界土壤磷(Pc)测试。研究表明,有效磷的变化和增加顺序为Olsen(2.19 ppm)
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引用次数: 0
Agronomic and environmental aspects of organo-mineral fertilizers produced with a by-product of the intermediate process of tanning 制革中间过程副产品生产的有机矿物肥料的农业和环境方面
Q2 Agricultural and Biological Sciences Pub Date : 2023-08-04 DOI: 10.3389/fagro.2023.1215448
F. Lima, A. O. Silva, Helen C. S. Amorim, Eduardo Sobrinho Santos Figueredo, Rafael Marta Carbone Carneiro, Madeliny Saracho Jara, M. Carneiro, L. Guilherme
The by-product of the intermediate process of tanning (BPIPT) can be used in the manufacturing of environmentally-friendly organo-mineral fertilizers (OMF). However, the presence of potentially toxic elements (e.g., chromium, Cr), can hinder the use of BPIPT in agriculture. This study aimed: i) to evaluate soil chemical and biological properties following the application of fertilizers produced with a BPIPT, in contrast to other OMF produced with traditional organic matrices; and ii) to assess the impacts of such products on wheat growth and nutrition.Samples (0-0.2 m) of two Oxisols [Red-Yellow Latosol (LVAd) and Red Latosol (LVd)] were used in this study, consisting of two experiments (with five treatments and five replicates) using different OMF, in a sequence: 1) incubation of fertilizers in soil samples for 30 days, and 2) wheat cultivation (30 days) after the fertilizer incubation. The treatments consisted of an arrangement of mineral fertilizers based on nitrogen, phosphorus, and potassium (NPK fertilizers), associated with OMF based on BPIPT (OM-IPT and OM-IPT+S) or commercial manure (OM-CM and OM-CM+S), with or without sulfur (S), and a control treatment. Elemental availability in soils and microbial attributes were determined after the incubation of the OMF in the soils. After wheat cultivation, plant biomass, nutritional composition, β-glucosidase activity, and fluorescein diacetate hydrolysis (FDA) were measured.The application of BPIPT-derived OMF in the present study increased available Cr fraction contents in both soils. However, OM-IPT caused low soil acidification, enhanced wheat growth and nutrition, and stimulated microbial activity in soils (FDA and β-glucosidase), thus evidencing the agronomic and environmental benefits of this OMF and their potential to contribute to a cleaner, more sustainable agriculture.
制革中间过程的副产品(BPIPT)可用于生产环保型有机无机肥料(OMF)。然而,潜在有毒元素(如铬)的存在会阻碍BPIPT在农业中的使用。本研究的目的是:i)评价施用BPIPT生产的肥料后土壤的化学和生物学特性,与使用传统有机基质生产的其他OMF相比;ii)评估这些产品对小麦生长和营养的影响。本研究采用两种氧化土[红-黄红土(LVAd)和红红土(LVd)]的样品(0-0.2 m),采用不同的OMF进行2个试验(5个处理,5个重复),顺序为:1)肥料在土壤样品中孵育30天,2)肥料孵育后种植小麦(30天)。这些处理包括氮磷钾(NPK)为主的矿物肥,与以BPIPT (OM-IPT和OM-IPT+S)或商业粪肥(OM-CM和OM-CM+S)为主的OMF(含硫或不含硫)和对照处理。在土壤中培养OMF后,测定了土壤中的元素有效性和微生物属性。小麦栽培后,测定植株生物量、营养成分、β-葡萄糖苷酶活性和双醋酸荧光素水解(FDA)。在本研究中,bpipt衍生的OMF的应用增加了两种土壤的有效铬含量。然而,OM-IPT降低了土壤酸化,提高了小麦的生长和营养,并刺激了土壤中的微生物活性(FDA和β-葡萄糖苷酶),从而证明了这种OMF的农学和环境效益,以及它们对更清洁、更可持续农业的贡献潜力。
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引用次数: 0
Evaluation of Philippine-sourced clay particles as coating agents of cacao pods and carrier of entomopathogen against cacao pest, Helopeltis bakeri Poppius 菲律宾来源的粘土颗粒作为可可荚包衣剂和昆虫病原体载体对抗可可害虫Helopeltis bakeri Poppius的评价
Q2 Agricultural and Biological Sciences Pub Date : 2023-08-04 DOI: 10.3389/fagro.2023.1213131
Sharin C. Albacete, D. Amalin, Thaddeus M. Carvajal, J. Wise
Evaluation of the efficacy of clay particles as a coating agent of cacao pods and carrier of entomopathogen, Metarhizium anisopliae Sorokin, was conducted for the control of cacao mirid bug (CMB), Helopeltis bakeri Poppius. Choice and no-choice tests were performed to evaluate Philippine-sourced clay particles as a coating agent of cacao pods to deter CMB feeding, in comparison with the commercially available particle film (US kaolin Surround®). To determine the most efficient local clay particles in protecting the pods from CMB feeding, six (6) treatments were evaluated namely, Philippine-sourced kaolin (PH kaolin), zeolite (PH zeolite), bentonite (PH bentonite), US kaolin, water (negative control), and a commercial synthetic insecticide thiamethoxam (Actara®) (positive control). All treatments were subjected to choice and no-choice tests. Among the Philippine clay particles tested in both tests, PH zeolite showed significant coating and deterred CMB from feeding. Since the US Kaolin and zeolite showed significant feeding deterrent effects on CMB, these treatments were tested as carriers of entomopathogenic fungi, M. anisopliae, including water (negative control) and thiamethoxam. Results showed that zeolite is a good carrier of the spores of M. anisopliae as its effects to deter CMB feeding started 24 hours after exposure. This was confirmed by positive M. anisopliae extraction from dead CMB through potato dextrose agar (PDA) plating.
研究了粘土颗粒作为可可豆荚包衣剂和绿僵菌载体对可可干虫(Helopeltis bakeri Poppius)的防治效果。通过选择和非选择试验,对菲律宾粘土颗粒作为可可豆荚包衣剂,与市售颗粒膜(美国高岭土环绕®)进行了比较,以评估其对CMB摄食的影响。为了确定保护豆荚免受CMB取食的最有效的本地粘土颗粒,对六种处理进行了评估,即菲律宾高岭土(PH高岭土)、沸石(PH沸石)、膨润土(PH膨润土)、美国高岭土、水(负对照)和商业合成杀虫剂噻虫胺(Actara®)(阳性对照)。所有的治疗都进行了选择和非选择测试。在两项试验中检测的菲律宾粘土颗粒中,PH沸石表现出明显的涂层,阻止了CMB的摄食。由于美国高岭土和沸石对CMB具有显著的摄食威慑作用,因此这些处理作为昆虫病原真菌绿僵菌的载体进行了测试,包括水(阴性对照)和噻虫嗪。结果表明,沸石是绿僵菌孢子的良好载体,其对绿僵菌取食的抑制作用在暴露24 h后开始。通过马铃薯葡萄糖琼脂(PDA)电镀从死亡的CMB中提取出阳性的金龟子分枝杆菌,证实了这一点。
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引用次数: 0
Investigating the effects of APSIM model configuration on model outputs across different environments 研究APSIM模型配置对不同环境下模型输出的影响
Q2 Agricultural and Biological Sciences Pub Date : 2023-07-31 DOI: 10.3389/fagro.2023.1213074
R. Chapagain, T. Remenyi, N. Huth, Caroline L. Mohammed, Jonathan J. Ojeda
Soil type plays a major role in nutrient dynamics and soil water which impacts crop growth and yield. The influence of soil characteristics on crop growth is usually evaluated through field experimentation (in the short term) and through crop-soil modelling (in the long-term). However, there has been limited research which has looked at the effect of model structural uncertainty of model outputs in different soil types.To analyze the impact of soil inputs on model structural uncertainty, we developed eight model structures (a combination of two crop models, two soil water models and two irrigation models) within the Agricultural Production Systems sIMulator (APSIM) across three soil types (Ferralsols, Alisols and Chernozems). By decomposing the mean proportion of variance and simulated values of the model outputs (yield, irrigation, drainage, nitrogen leaching and partial gross margin) we identified the influence of soil type on the magnitude of model structural uncertainty.For all soil types, crop model was the most significant source of structural uncertainty, contributing >60% to variability for most modelled variables, except irrigation demand which was dominated by the choice of irrigation model applied. Relative to first order interactions, there were minimal (<12%) contributions to uncertainty from the second order interactions (i.e., inter-model components). We found that a higher mean proportion of variance does not necessarily imply a high magnitude of uncertainty in actual values. Despite the significant impact of the choice of crop model on yield and PGM variance (contributing over 90%), the small standard deviations in simulated yield (ranging from 0.2 to 1 t ha-1) and PGM (ranging from 50.6 to 374.4 USD ha-1) compared to the mean values (yield: 14.6 t ha-1, PGM: 4901 USD ha-1) indicate relatively low actual uncertainty in the values. Similarly, the choice of irrigation model had a contribution of over 45% to variance, but the relatively small standard deviations ranging from 11 to 33.3 mm compared to the overall mean irrigation of 500 mm suggest low actual uncertainty in the values. In contrast, for the environmental variables- drainage and nitrogen leaching, the choice of crop model had contributions of more than 60% and 70% respectively, yet the relatively large standard deviations ranging from 7.1 to 30.6 mm and 0.6 to 7.7 kg ha-1 respectively, compared to the overall mean values of drainage (44.4 mm) and nitrogen leaching (3.2 kg ha-1), indicate significant actual uncertainty.We identified the need to include not only fractional variance of model uncertainty, but also magnitude of the contribution in measured units (e.g. t ha-1, mm, kg ha-1, USD ha-1) for crop model uncertainty assessments to provide more useful agronomic or policy decision-making information. The findings of this study highlight the sensitivity of agricultural models to the impacts of moisture availability, suggesting that it is important to give more attention
土壤类型在影响作物生长和产量的养分动态和土壤水分中起着重要作用。土壤特性对作物生长的影响通常通过田间试验(短期)和作物土壤建模(长期)进行评估。然而,研究不同土壤类型下模型输出的模型结构不确定性的影响的研究有限。为了分析土壤输入对模型结构不确定性的影响,我们在农业生产系统模拟器(APSIM)中开发了八个模型结构(两个作物模型、两个土壤水分模型和两个灌溉模型的组合),涵盖三种土壤类型(Ferralsol、Alisols和Chernozems)。通过分解模型输出(产量、灌溉、排水、氮浸出和部分毛利率)的平均方差比例和模拟值,我们确定了土壤类型对模型结构不确定性大小的影响。对于所有土壤类型,作物模型是结构不确定性的最重要来源,对大多数建模变量的变异性贡献>60%,灌溉需求除外,灌溉需求主要由所用灌溉模型的选择决定。相对于一阶相互作用,二阶相互作用(即模型间组件)对不确定性的贡献最小(<12%)。我们发现,方差的平均比例越高,并不一定意味着实际值的不确定性越大。尽管作物模型的选择对产量和PGM方差有显著影响(贡献率超过90%),但与平均值(产量:14.6吨ha-1,PGM:4901美元ha-1)相比,模拟产量(范围为0.2至1吨ha-1)和PGM(范围为50.6至374.4美元ha-1。同样,灌溉模型的选择对方差的贡献超过45%,但与500毫米的总体平均灌溉相比,11至33.3毫米的相对较小的标准偏差表明,这些值的实际不确定性较低。相反,对于环境变量——排水和氮浸出,作物模型的选择分别贡献了60%和70%以上,但与排水(44.4 mm)和氮浸出(3.2 kg ha-1)的总体平均值相比,相对较大的标准偏差分别为7.1至30.6 mm和0.6至7.7 kg ha-1,表示显著的实际不确定性。我们确定,不仅需要包括模型不确定性的分数方差,还需要包括作物模型不确定性评估的测量单位(如t ha-1,mm,kg ha-1,USD ha-1)的贡献大小,以提供更有用的农艺或政策决策信息。这项研究的结果突出了农业模型对水分可用性影响的敏感性,表明在根据分析的产量对干湿条件进行建模时,更多地关注结构不确定性很重要。
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引用次数: 0
Modeling the early phases of epidemics by Phakospora pachyrhizi in Brazilian soybean 巴西大豆中厚叶Phakospora pachyrhizi流行病早期阶段的建模
Q2 Agricultural and Biological Sciences Pub Date : 2023-07-31 DOI: 10.3389/fagro.2023.1214038
B. Kassie, D. Onstad, L. Koga, Tim Hart, R. Clark, G. W. van der Heijden
Asian soybean rust, caused by the biotrophic basidiomycete Phakospora pachyrhizi, is a foliar disease that often causes considerable damage to soybean crops. The purpose of our work was to create a mechanistic model that can reliably represent epidemics of ASR in commercial soybean fields in Brazil. The most important inputs for the model are weather data (observations and forecast) and the initial observation of disease (or uredospore arrival). Our focus is on the first two or three cycles of infection after immigration into a soybean field. The model includes state variables for latent, infectious and senesced lesions, disease severity, uredospores, and soybean leaf area. Processes modeled include maturation through the latent and infectious periods, germination, sporulation, and processes affecting uredospores in the canopy. The model results were tested against field observations from trials at four locations in Brazil for the 2019/2020 growing season. The predictions generally matched the daily dynamics of disease progress in the field trials. The predictions reproduced the observed severity well with R2 value of 0.84. This high correlation indicates that our model is accurate enough to be used as a tool to predict the dynamics of ASR epidemics during the first few cycles after uredospore invasion into a soybean field. A sensitivity analysis was performed that showed that the model is sensitive to time and duration of the initial spore arrival. This indicates that spore traps or other observations should measure not only the first day of arrival but also subsequent days.
亚洲大豆锈病是由富营养担子菌Phakospora pachyrhizi引起的一种叶面病,通常会对大豆作物造成相当大的损害。我们工作的目的是创建一个能够可靠地代表巴西商业大豆田ASR流行病的机制模型。该模型最重要的输入是天气数据(观测和预测)和疾病的初始观测(或脲孢子虫到达)。我们的重点是移民到大豆田后的前两三个感染周期。该模型包括潜伏性、感染性和衰老性病变、疾病严重程度、尿素孢子和大豆叶面积的状态变量。建模的过程包括通过潜伏期和感染期的成熟、发芽、孢子形成以及影响冠层中尿素孢子的过程。模型结果与巴西2019/2020年生长季节四个地点的试验现场观测结果进行了对比测试。这些预测通常与现场试验中疾病进展的每日动态相匹配。预测很好地再现了观察到的严重性,R2值为0.84。这种高度相关性表明,我们的模型足够准确,可以用作预测尿素孢子入侵大豆田后最初几个周期ASR流行病动态的工具。敏感性分析表明,该模型对孢子初始到达的时间和持续时间敏感。这表明孢子捕获器或其他观测不仅应测量到达的第一天,还应测量随后的几天。
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引用次数: 0
Genomic analysis of Sinorhizobium meliloti LPU63, an acid-tolerant and symbiotically efficient alfalfa-nodulating rhizobia 耐酸共生高效苜蓿结瘤根瘤菌meliloti中华根瘤菌LPU63的基因组分析
Q2 Agricultural and Biological Sciences Pub Date : 2023-07-27 DOI: 10.3389/fagro.2023.1175524
J. H. Cafiero, María Salvetti Casasco, M. Lozano, C. Vacca, Silvina Laura López García, W. Draghi, A. Lagares, M. F. Del Papa
The growth and persistence of alfalfa (Medicago sativa), a perennial legume capable of producing high yields of high-quality forage, is reduced in moderately acidic soils. The low performance of alfalfa at low pH is due to numerous factors that affect the host plant, their rhizobia, and the symbiotic interaction. Sinorhizobium meliloti LPU63 was isolated from acid topsoil (in Argentina) and showed to be a highly competitive and efficient N2-fixing rhizobium under both neutral and moderately acidic soil conditions. In this study, we obtained a draft of the LPU63 genome sequence using Illumina HiSeq4000. The whole genome phylogenetic analysis confirmed the taxonomic position of LPU63 as a S. meliloti strain and the multilocus sequence analysis confirmed that LPU63 is not related to the strains used in Argentina in bioformulations. The genomic analysis showed that beyond the canonical chromosome, pSymA, and pSymB, LPU63 strain has an accessory plasmid that codes for a repABC origin of replication and a conjugative T4SS, suggesting that this plasmid could be self-transmissible. In addition, the complete denitrification pathway (i.e., the gene clusters nap, nir, nor, and nos), including napC and nosZ, which could be used as an alternative respiration route under hypoxic conditions with moderate N2O emissions was found. Also, genes associated with plant growth-promoting activities (PGPR) and the degradation of phenylacetic acid (PAA) were identified. LPU63 is a highly melanogenic strain, a property that could enhance its survival under soil conditions, and the genome data showed a particular arrangement of the genes involved in melanin production. The information regarding LPU63 activities compatible with plant-growth promotion phenotypes, together with other characteristics mentioned here (melanin production, potential moderate N2O emissions), constitute the basis of future experiments toward the rational design of a novel bioinoculant for the environmentally sustainable production of alfalfa.
苜蓿(Medicago sativa)是一种多年生豆科植物,能够生产高质量的牧草,在中等酸性土壤中生长和持久性降低。紫花苜蓿在低pH下的低生产性能是由于多种因素影响了寄主植物、它们的根瘤菌和共生相互作用。从阿根廷酸性表土中分离到Sinorhizobium meliloti LPU63,在中性和中酸性土壤条件下均表现出高度竞争性和高效的固氮根瘤菌。在这项研究中,我们使用Illumina HiSeq4000获得了LPU63基因组序列的草图。全基因组系统发育分析证实了LPU63为S. meliloti菌株,多位点序列分析证实LPU63与阿根廷生物制剂中使用的菌株无亲缘关系。基因组分析表明,除了典型染色体pSymA和pSymB外,LPU63菌株还有一个辅助质粒,该质粒编码复制的repABC起源和共轭T4SS,表明该质粒可能具有自传性。此外,还发现了完整的反硝化途径(即nap、nir、nor和nos基因簇),包括napC和nosZ,可以作为缺氧条件下适度N2O排放的替代呼吸途径。此外,还鉴定了与植物生长促进活性(PGPR)和苯乙酸(PAA)降解相关的基因。LPU63是一种高度黑色素生成菌株,这种特性可以提高其在土壤条件下的存活率,基因组数据显示了与黑色素生成有关的基因的特殊排列。有关LPU63活性与植物生长促进表型相容的信息,以及本文提到的其他特性(黑色素产生,潜在的适度N2O排放),构成了未来合理设计新型生物接种剂用于环境可持续生产苜蓿的实验基础。
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引用次数: 0
Reinforcing the Hydrosocial Cycle to foster water governance and stakeholders’ interdependence in urban agroecosystems: a local test in Benidorm, Spain 加强水社会循环,促进城市农业生态系统中的水治理和利益相关者的相互依存:西班牙贝尼多姆的地方试验
Q2 Agricultural and Biological Sciences Pub Date : 2023-07-27 DOI: 10.3389/fagro.2023.1057211
S. Ricart, R. Villar, M. Hernández-Hernández, Antonio M. Rico-Amorós, J. Olcina-Cantos, C. Baños
The Hydrosocial Cycle (HSC) has been widely applied and discussed as a consolidated research line to rethink the contemporary challenges that condition the urban and agroecosystem nexus. However, additional research directions are still open to guide policy and decision-makers in reinforcing stakeholders’ engagement and interaction to resolve tensions between water demands. This perspective paper suggests updating the HSC approach to improve the analysis of stakeholder interaction when addressing water scarcity in waterscapes. After briefly review the most relevant contributions of the HSC approach in the last two decades, we develop a preliminary framework to reinforce stakeholders’ interdependence analysis by designing a questionnaire to synthesize five main behavioral patterns conditioning stakeholders’ interactions: relevance, representativeness, recognition, assessment, and collaboration. Then, each pattern is organized in a triple-loop approach: to be, to do, and to share to characterize the mutual (mis)understanding of the stakeholders. The results of its application to Benidorm (south of Spain), a mass-tourism destination coexisting with rural development in tension for water supply, exemplified how 1) most stakeholders consider themselves important, but some of them are unaware of the role of others, 2) all stakeholders receive a higher punctuation in terms of functions rather than actions, and 3) all stakeholders agree on the benefits of the predisposition of parties (willingness) to achieve agreements in the short or medium term. Future research should consider how to address the lack of representativeness and power imbalance together with mechanisms to reinforce longitudinal studies in which actions from stakeholders could be contrasted.
水文社会循环(HSC)作为一条综合研究路线,已被广泛应用和讨论,以重新思考制约城市和农业生态系统关系的当代挑战。然而,仍有更多的研究方向可以指导政策制定者和决策者加强利益相关者的参与和互动,以解决用水需求之间的紧张关系。这篇前瞻性论文建议更新HSC方法,以改进在解决水景缺水问题时对利益相关者互动的分析。在简要回顾了HSC方法在过去二十年中最相关的贡献后,我们制定了一个初步框架,通过设计一份问卷来综合影响利益相关者互动的五种主要行为模式:相关性、代表性、认可、评估和协作,来加强利益相关者的相互依存性分析。然后,每种模式都以三重循环的方式组织:成为、做和共享,以表征利益相关者的相互(错误)理解。贝尼多姆(西班牙南部)是一个与供水紧张的农村发展共存的大众旅游目的地,其应用结果表明:1)大多数利益相关者认为自己很重要,但其中一些人不知道其他人的作用,3)所有利益攸关方都同意各方在短期或中期内达成协议的倾向(意愿)带来的好处。未来的研究应考虑如何解决缺乏代表性和权力失衡的问题,以及加强纵向研究的机制,在纵向研究中可以对比利益相关者的行动。
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引用次数: 0
A newly isolated cotton-infecting Polerovirus with cryptic pathogenicity encodes a weak suppressor of RNA silencing 一种新分离的棉花感染病毒具有隐性致病性,编码RNA沉默的弱抑制子
Q2 Agricultural and Biological Sciences Pub Date : 2023-07-24 DOI: 10.3389/fagro.2023.1235168
M. Akinyuwa, Bailee K. Price, K. Martin, Sung-Hwan Kang
Cotton is a multipurpose crop grown globally, including the United States. Cotton leafroll dwarf virus (CLRDV), a phloem-limited virus (Solemoviridae) transmitted by aphids, causes significant economic losses to cotton cultivation. CLRDV strains (CLRDV-typical and atypical) that were previously prevalent in other countries cause severe symptoms leading to high yield loss. Recently, a new isolate of CLRDV (CLRDV-AL) has been characterized from infected cotton plants in Alabama that are often asymptomatic and difficult to detect, implying a low titer and pathogenicity within the host. Different pathogenicity among certain strains within the same species often correlates with both environmental and molecular factors. Thus, better management and control of the vector-borne disease can be achieved by elucidating host-pathogen interaction, such as host immune response and pathogen counter-response. In this study, we demonstrate the ability of CLRDV-AL to suppress a major host defense response known as RNA silencing and compare the potency of silencing suppression to other strains of the same virus. Also, we discuss the difference in pathogenicity among them by evaluating the observations based on the amino acid variation within the functional domain. Our study provides and suggests a future direction for specifying the strategy to mitigate potential cotton disease severity.
棉花是包括美国在内的全球种植的多用途作物。棉花卷叶矮病毒(CLRDV)是一种由蚜虫传播的韧皮部限制性病毒(Solemoviridae),给棉花种植造成了重大的经济损失。以前在其他国家流行的CLRDV菌株(典型和非典型CLRDV)会引起严重症状,导致高产损失。最近,一种新的CLRDV分离株(CLRDV-AL)已从阿拉巴马州受感染的棉花植株中鉴定出来,这些植株通常无症状且难以检测,这意味着其在宿主体内的滴度和致病性较低。同一物种中某些菌株的不同致病性通常与环境和分子因素有关。因此,通过阐明宿主-病原体的相互作用,如宿主免疫反应和病原体对抗反应,可以实现对媒介传播疾病的更好管理和控制。在这项研究中,我们证明了CLRDV-AL抑制被称为RNA沉默的主要宿主防御反应的能力,并将沉默抑制的效力与同一病毒的其他毒株进行了比较。此外,我们通过评估基于功能域内氨基酸变异的观察结果,讨论了它们之间的致病性差异。我们的研究为确定减轻棉花潜在疾病严重程度的策略提供了并提出了未来的方向。
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引用次数: 0
Emerging possibilities in the advancement of herbicides to combat acetyl-CoA carboxylase inhibitor resistance 除草剂对抗乙酰辅酶a羧化酶抑制剂抗性的新可能性
Q2 Agricultural and Biological Sciences Pub Date : 2023-07-20 DOI: 10.3389/fagro.2023.1218824
Bikash Kumar Rajak, Priyanka Rani, Pranabesh Mandal, R. S. Chhokar, Nitesh Singh, Durg Vijay Singh
Acetyl-CoA carboxylase (ACCase: EC 6.4.1.2) is one of the essential biotins containing enzymes required by plants for fatty acid synthesis and elongation. The unique enzyme is present in its homomeric form in all the Gramineae family, making it a suitable target for developing herbicides selectively against weeds of the Gramineae family. One such example is infestation of Phalaris minor in winter wheat crop fields, where aryloxyphenoxypropionates (FOP); cyclohexanediones (DIM) and phenyl pyrazoline (DEN) group of ACCase inhibiting herbicides are used. However, the increasing number of ACCase herbicide resistant weed populations has compelled agro-scientists to seek varied possibilities for weed control, through Integrated Weed Management (IWM) strategies. Developing new potential herbicides to regain sensitivity in weeds could be an approach to weed control. The current advancement in computational techniques could be of aid in developing new herbicide-like molecules by exploring the genomics, proteomics and structural details of catalytic sites of herbicide action in crops as well as weeds.
乙酰辅酶a羧化酶(Acetyl-CoA carboxylase, ACCase: EC 6.4.1.2)是植物合成和伸长脂肪酸所必需的含生物素酶之一。这种独特的酶在所有禾本科植物家族中都以同质形式存在,使其成为开发针对禾本科杂草的除草剂的合适靶点。一个这样的例子是在冬小麦作物田里的小Phalaris侵扰,其中芳氧苯氧丙酸酯(FOP);采用环己二酮类(DIM)和苯基吡唑啉类(DEN)抗ACCase除草剂。然而,越来越多的抗ACCase除草剂杂草种群迫使农业科学家寻求通过综合杂草管理(IWM)策略来控制杂草的各种可能性。开发新的潜在除草剂来恢复杂草的敏感性可能是控制杂草的方法。当前计算技术的进步可以通过探索作物和杂草中除草剂作用催化位点的基因组学、蛋白质组学和结构细节来帮助开发新的类除草剂分子。
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引用次数: 1
Poultry litter and cow dung biochar as P sources for cowpea cultivation in two Ghanaian soils 家禽粪便和牛粪生物炭作为两种加纳土壤中豇豆种植的磷源
Q2 Agricultural and Biological Sciences Pub Date : 2023-07-20 DOI: 10.3389/fagro.2023.1233255
E. Nartey, N. Sulemana, Awudu Razak, T. Adjadeh, A. M. Akumah, C. Amoatey, M. Abekoe
The main constraint to cowpea, Vigna unguiculata, production in West Africa is unavailability of applied phosphorus due to ligand exchange with clay minerals and precipitation reactions facilitated by low pH and low organic matter.To overcome this challenge, cow dung biochar (CB) and poultry litter biochar (PB) were applied as P sources for cowpea cultivation in Plinthustult and Kandiustalf soil in Ghana. The biochar types and triple super phosphate (TSP) were applied to meet the standard phosphorus requirement (SPR) and ½ SPR of the soils. Basal K from KCl was added to the TSP treatment. In addition to a non-amended soil, the treatments were triplicated and arranged in a completely randomized design in a screen house for an efficacy trial using cowpea as the test crop. Moisture content was maintained at 80% field capacity.Hundred cowpea seed weight was 20.3 g and 19.6 g for the TSP-amended Plinthustult at SPR and ½ SPR, respectively. This increased to 25.1 g and approximately 27 g at SPR and ½ SPR in the CB- and PB-amended Plinthustult, respectively. Hundred seed weight for TSP at both rates was similar in the two soils. The PB-amended Kandiustalf at SPR had seed with a weight of 27.02 g, 1.1 g heavier than the ½ SPR. The CB-amended Kandiustalf at both rates had 100 seeds weighing 25 g. Residual available P was 334.2 mg/kg and 213.2 mg/kg at SPR and ½ SPR, respectively, in the Plinthustult as opposed to a paltry 2.5 mg/kg at SPR in the TSP counterpart. The study recommends for the two biochar types to be applied at ½ SPR.
西非豇豆(Vigna unguiculata)生产的主要制约因素是,由于与粘土矿物的配体交换以及低pH和低有机物促进的沉淀反应,无法获得施用的磷。为了克服这一挑战,在加纳的Plinthustult和Kandiustalf土壤中,牛粪生物炭(CB)和家禽粪便生物炭(PB)被用作豇豆种植的磷源。应用生物炭类型和三重超磷酸盐(TSP)来满足土壤的标准磷需求(SPR)和½SPR。将KCl中的碱性钾添加到TSP处理中。除了未改良的土壤外,这些处理是三重的,并在筛选室中以完全随机的设计进行安排,以进行以豇豆为试验作物的药效试验。水分含量保持在80%的田间容量。在SPR和½SPR条件下,TSP修正的Plinthustult的百粒豇豆种子重量分别为20.3g和19.6g。在CB和PB修正的Plinthustult中,SPR和½SPR时,这分别增加到25.1克和约27克。在两种土壤中,TSP在两种速率下的百粒重相似。在SPR条件下,PB改良的Kandiustalf的种子重量为27.02 g,比½SPR重1.1 g。在两种速率下,CB改良的Kandiustalf都有100颗种子,重量为25 g。在SPR和½SPR条件下的Plinthustult中,残留有效磷分别为334.2 mg/kg和213.2 mg/kg,而在TSP条件下,SPR条件下残留有效磷仅为微不足道的2.5 mg/kg。该研究建议在½SPR条件下使用两种生物炭。
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引用次数: 0
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Frontiers in Agronomy
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