Pub Date : 2022-06-14DOI: 10.31016/1998-8435-2022-16-2-243-251
O. Tochieva, M. Arisov
The purpose of the research is to study the therapeutic efcacy of drugs in the form of a solution for external use based on imidacloprid, pyriproxyfen and moxidectin against gastrointestinal nematodes and Diroflaria spp. microflaria in dogs and cats.Materials and methods. . The studies were carried out on the basis of VNIIP (fl. Federal State Budgetary Scientifc Institution FNTs VIEV RAS) on dogs and cats of different sex and age, body weight spontaneously infected with parasites. The diagnosis, as well as the effectiveness of the drugs, was confrmed in a complex manner based on the anamnestic and epizootological data, the clinical picture and laboratory research methods (detection of helminth eggs in the animal's feces using the Fülleborn method with subsequent differentiation, microscopy of animal blood smears to detect microflariae). To rule out infection of animals (dogs) with babesia, blood samples were taken and a laboratory analysis was performed to detect parasites (Babesia canis).Results and discussion. 100% effectiveness of Insacar Total C (for dogs) and Insacar Total K (for cats) has been established for nematodes of the gastrointestinal tract (toxocarosis, toxascariosis, uncinariosis, hookworm, trichuriosis) and diroflariosis (the initial stage of the disease). Side effects and complications after treatment of animals with drugs were not revealed
本研究的目的是研究以吡虫啉、吡嘧磺酰和莫西定为基础的外用溶液形式的药物对胃肠道线虫和核黄素的治疗效果。材料和方法。这些研究是在VNIIP（联邦国家预算科学机构FNTs VIEV RAS）的基础上对不同性别、年龄、体重的狗和猫自发感染寄生虫进行的。诊断以及药物的有效性是以一种复杂的方式根据记忆和流行病数据、临床图片和实验室研究方法（使用Fülleborn方法检测动物粪便中的蠕虫卵，随后进行鉴别，动物血涂片显微镜检查以检测微疟）来确定的。为了排除巴贝西亚感染动物（狗）的可能性，我们采集了血液样本，并进行了实验室分析，以检测寄生虫（犬巴贝西亚）。结果和讨论。Insacar Total C（用于狗）和Insacar Total K（用于猫）已被证明对胃肠道线虫（毒蕈病、毒虫病、钩虫病、毛毛虫病）和腹泻病（疾病的初始阶段）有100%的疗效。药物治疗动物后未发现副作用和并发症
{"title":"Therapeutic efcacy of preparations in the form of a solution for external use based on imidacloprid, pyriproxyfen and moxidectin at nematodosis of dogs and cats","authors":"O. Tochieva, M. Arisov","doi":"10.31016/1998-8435-2022-16-2-243-251","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-243-251","url":null,"abstract":"The purpose of the research is to study the therapeutic efcacy of drugs in the form of a solution for external use based on imidacloprid, pyriproxyfen and moxidectin against gastrointestinal nematodes and Diroflaria spp. microflaria in dogs and cats.Materials and methods. . The studies were carried out on the basis of VNIIP (fl. Federal State Budgetary Scientifc Institution FNTs VIEV RAS) on dogs and cats of different sex and age, body weight spontaneously infected with parasites. The diagnosis, as well as the effectiveness of the drugs, was confrmed in a complex manner based on the anamnestic and epizootological data, the clinical picture and laboratory research methods (detection of helminth eggs in the animal's feces using the Fülleborn method with subsequent differentiation, microscopy of animal blood smears to detect microflariae). To rule out infection of animals (dogs) with babesia, blood samples were taken and a laboratory analysis was performed to detect parasites (Babesia canis).Results and discussion. 100% effectiveness of Insacar Total C (for dogs) and Insacar Total K (for cats) has been established for nematodes of the gastrointestinal tract (toxocarosis, toxascariosis, uncinariosis, hookworm, trichuriosis) and diroflariosis (the initial stage of the disease). Side effects and complications after treatment of animals with drugs were not revealed","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46597777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.31016/1998-8435-2022-16-2-160-169
R. Safiullin, E. Chalysheva
The purpose of the research is to study the spread of coccidia infections in turkey poults on industrial turkey farms in the Central Region of Russia.Materials and methods. Turkey poults were studied for coccidiosis by the following life-time diagnostic methods: coproscopic examinations according to Darling, McMaster and Fülleborn. The material to evaluate protozoal diseases spreading in turkey poults was results of our own laboratory studies of the biomaterial (dung samples) and carcass dissection fndings. When studying the age dynamics of the turkey poults’ infection with Eimeria spp. on the poultry farms in the Central Region, young birds aged 7 days and older were studied until the completion of the technological cycle of production, every 14 days, by examining at least 20 fresh dung samples and 10 scrapings from the floor. To determine the seasonal dynamics of infection, the samples were taken from the poultry buildings in January in winter season, April in Spring, July in Summer, and October in Autumn.Results and discussion. The highest coccidia infection rate was detected at the age of 35–49 days in young males (Infection Prevalence, 30–45%), and 35–63 days in females (Infection Prevalence, 5–30%). The turkey poults were infected with Eimeria spp. throughout the year with minor seasonal variations. The young birds’ age had a more noticeable effect on the infection rate in the turkey poults. The external environment (floor, walls, and feeders) was contaminated with infective coccidia elements by 5–31.3%.
{"title":"Coccidiosis of turkeys on industrial farms in the Central Region of Russia","authors":"R. Safiullin, E. Chalysheva","doi":"10.31016/1998-8435-2022-16-2-160-169","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-160-169","url":null,"abstract":"The purpose of the research is to study the spread of coccidia infections in turkey poults on industrial turkey farms in the Central Region of Russia.Materials and methods. Turkey poults were studied for coccidiosis by the following life-time diagnostic methods: coproscopic examinations according to Darling, McMaster and Fülleborn. The material to evaluate protozoal diseases spreading in turkey poults was results of our own laboratory studies of the biomaterial (dung samples) and carcass dissection fndings. When studying the age dynamics of the turkey poults’ infection with Eimeria spp. on the poultry farms in the Central Region, young birds aged 7 days and older were studied until the completion of the technological cycle of production, every 14 days, by examining at least 20 fresh dung samples and 10 scrapings from the floor. To determine the seasonal dynamics of infection, the samples were taken from the poultry buildings in January in winter season, April in Spring, July in Summer, and October in Autumn.Results and discussion. The highest coccidia infection rate was detected at the age of 35–49 days in young males (Infection Prevalence, 30–45%), and 35–63 days in females (Infection Prevalence, 5–30%). The turkey poults were infected with Eimeria spp. throughout the year with minor seasonal variations. The young birds’ age had a more noticeable effect on the infection rate in the turkey poults. The external environment (floor, walls, and feeders) was contaminated with infective coccidia elements by 5–31.3%.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44403124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.31016/1998-8435-2022-16-2-147-153
O. M. Bonina, S. A. Zuykov
The purpose of the research is identifcation of epidemically and epizootically dangerous fsh species for opisthorchosis in the Novosibirsk Region.Materials and methods. Helminthological studies were carried out from 2002 to 2020. We studied a total of 2994 fsh specimens belonging to 8 species of Cyprinidae, carps and allies, namely, ide, Leuciscus idus (L.); dace, L. leuciscus (L.); bream, Abramis brama (L.); roach, Rutilus rutilus (L.); verkhovka, Leucaspius delineatus (Heckel); crucian carp, Carassius carassius (L.); gudgeon, Gobio gobio (L.); and minnow, Phoxinus phoxinus (L.). The fsh were identifed to species according to the fsh guide. To identify Opisthorchidae metacercariae in fsh muscles, the compressor method generally accepted in helminthology was used. The Opisthorchidae metacercariae were identifed using the freshwater fsh parasite guide.Results and discussion. Of 8 studied fsh species of the family Cyprinidae in the Novosibirsk region, the Opisthorchidae metacercariae infection was noted in 7 species (ide, dace, roach, bream, verkhovka, gudgeon, and crucian carp). The following Opisthorchidae species were recorded in the studied fsh: O. felineus, M. bilis, M. xanthosomus, and Metorchis spp. The O. felineus metacercariae were most often found in the supplementary host, in 12.9 % of cases, and M. bilis, M. xanthosomus and Metorchis spp. larvae were found much less often. In terms of epidemy, the ide and bream having large commercial sizes are the most dangerous: the ide as the most heavily infected species, and the bream as the most often caught and eaten by humans. Small non-commercial fsh (the dace, roach, verkhovka, as well as the small ide and bream) are of great epizootological importance, since they are used for food for domestic and wild animals most often. Infected fsh were only found at unregulated marketplaces; the infection prevalence in the roach was 35.5%, of which 22.6% were affected by O. felineus metacercariae, and 12.9% by M. xanthosomus metacercariae.
{"title":"Epidemically and epizootically dangerous fsh species for opisthorchosis in the Novosibirsk Region","authors":"O. M. Bonina, S. A. Zuykov","doi":"10.31016/1998-8435-2022-16-2-147-153","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-147-153","url":null,"abstract":"The purpose of the research is identifcation of epidemically and epizootically dangerous fsh species for opisthorchosis in the Novosibirsk Region.Materials and methods. Helminthological studies were carried out from 2002 to 2020. We studied a total of 2994 fsh specimens belonging to 8 species of Cyprinidae, carps and allies, namely, ide, Leuciscus idus (L.); dace, L. leuciscus (L.); bream, Abramis brama (L.); roach, Rutilus rutilus (L.); verkhovka, Leucaspius delineatus (Heckel); crucian carp, Carassius carassius (L.); gudgeon, Gobio gobio (L.); and minnow, Phoxinus phoxinus (L.). The fsh were identifed to species according to the fsh guide. To identify Opisthorchidae metacercariae in fsh muscles, the compressor method generally accepted in helminthology was used. The Opisthorchidae metacercariae were identifed using the freshwater fsh parasite guide.Results and discussion. Of 8 studied fsh species of the family Cyprinidae in the Novosibirsk region, the Opisthorchidae metacercariae infection was noted in 7 species (ide, dace, roach, bream, verkhovka, gudgeon, and crucian carp). The following Opisthorchidae species were recorded in the studied fsh: O. felineus, M. bilis, M. xanthosomus, and Metorchis spp. The O. felineus metacercariae were most often found in the supplementary host, in 12.9 % of cases, and M. bilis, M. xanthosomus and Metorchis spp. larvae were found much less often. In terms of epidemy, the ide and bream having large commercial sizes are the most dangerous: the ide as the most heavily infected species, and the bream as the most often caught and eaten by humans. Small non-commercial fsh (the dace, roach, verkhovka, as well as the small ide and bream) are of great epizootological importance, since they are used for food for domestic and wild animals most often. Infected fsh were only found at unregulated marketplaces; the infection prevalence in the roach was 35.5%, of which 22.6% were affected by O. felineus metacercariae, and 12.9% by M. xanthosomus metacercariae.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41344746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.31016/1998-8435-2022-16-2-170-176
D. S. Suslo
The purpose of the research is to study of larval biotopes of the family Culicidae Meigen, 1818 on the territory of the Berezinsky Biosphere Reserve.Materials and methods. Mosquito larvae collected on the Berezinsky Biosphere Reserve in 2016–2020. A total of 7772 mosquito larvae were collected in the course of 1467 counts. Mosquito larvae were collected in four types of natural reservoirs: permanent open (I); permanent shaded (II); temporary open (III) and temporary shaded (IV). The larvae were collected by dip net. The specimens were identifed using standard keys; in certain cases, the taxonomic collection of the Zoological Institute of the Russian Academy of Sciences (St. Petersburg) was used.Results and discussion.We found larvae of 22 mosquito species on the Berezinsky Biosphere Reserve. The genus Aedes Meigen, 1818 is the most abundant – 16 species (72,7%). The genus Anopheles Meigen, 1818 is represented by 2 species (9,2%), the genus Culiseta Felt, 1904 – 3 species (13,6%) and the genus Culex Linnaeus, 1758 – 1 species (4,5%). The largest number of species (18) is recorded in permanent and temporary open-type reservoirs, 17 – in temporary shaded reservoirs and the smallest number – 6 in permanent shaded reservoirs. Three species, Aedes vexans (Meigen, 1830), Ae. cantans (Meigen, 1818), and Ae. sticticus (Meigen, 1838) were found at all types of water bodies. The main larval breeding habitats are reservoirs of temporary origin with varying degrees of shading (88,0%). There are the highest abundance and mean average density of larvae. Thus, in temporary open water bodies, the abundance is 52,4% (with an average density of 197,0±45,7 sp./m2), and in temporary shaded water bodies, the abundance is 35,5% (with an average density of 287,2±162,1 sp./m2). For permanent reservoirs with varying degrees of shading, a low abundance and an average density of larvae are noted. Thus, in permanent open water bodies, the abundance is 9,6% (with an average density of 27,0±7,7 sp./m2), and in permanent shaded water bodies, the abundance is 2,5% (with an average density of 19,4±6,0 sp./m2). In permanent open water bodies, the greatest species diversity is noted, which is confrmed by the values of the indices (Нʹ = 3,06; D Mg = 2,57 and DSm = 0,16).
{"title":"Larval biotopes of mosquitoes (Diptera: Culicidae) on the Berezinsky Biosphere Reserve (Belarus)","authors":"D. S. Suslo","doi":"10.31016/1998-8435-2022-16-2-170-176","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-170-176","url":null,"abstract":"The purpose of the research is to study of larval biotopes of the family Culicidae Meigen, 1818 on the territory of the Berezinsky Biosphere Reserve.Materials and methods. Mosquito larvae collected on the Berezinsky Biosphere Reserve in 2016–2020. A total of 7772 mosquito larvae were collected in the course of 1467 counts. Mosquito larvae were collected in four types of natural reservoirs: permanent open (I); permanent shaded (II); temporary open (III) and temporary shaded (IV). The larvae were collected by dip net. The specimens were identifed using standard keys; in certain cases, the taxonomic collection of the Zoological Institute of the Russian Academy of Sciences (St. Petersburg) was used.Results and discussion.We found larvae of 22 mosquito species on the Berezinsky Biosphere Reserve. The genus Aedes Meigen, 1818 is the most abundant – 16 species (72,7%). The genus Anopheles Meigen, 1818 is represented by 2 species (9,2%), the genus Culiseta Felt, 1904 – 3 species (13,6%) and the genus Culex Linnaeus, 1758 – 1 species (4,5%). The largest number of species (18) is recorded in permanent and temporary open-type reservoirs, 17 – in temporary shaded reservoirs and the smallest number – 6 in permanent shaded reservoirs. Three species, Aedes vexans (Meigen, 1830), Ae. cantans (Meigen, 1818), and Ae. sticticus (Meigen, 1838) were found at all types of water bodies. The main larval breeding habitats are reservoirs of temporary origin with varying degrees of shading (88,0%). There are the highest abundance and mean average density of larvae. Thus, in temporary open water bodies, the abundance is 52,4% (with an average density of 197,0±45,7 sp./m2), and in temporary shaded water bodies, the abundance is 35,5% (with an average density of 287,2±162,1 sp./m2). For permanent reservoirs with varying degrees of shading, a low abundance and an average density of larvae are noted. Thus, in permanent open water bodies, the abundance is 9,6% (with an average density of 27,0±7,7 sp./m2), and in permanent shaded water bodies, the abundance is 2,5% (with an average density of 19,4±6,0 sp./m2). In permanent open water bodies, the greatest species diversity is noted, which is confrmed by the values of the indices (Нʹ = 3,06; D Mg = 2,57 and DSm = 0,16).","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43745999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.31016/1998-8435-2022-16-2-154-159
E. A. Detkova, S. Lutsuk
The purpose of the research is to determine factors affecting the spread rate of thelaziosis in beef breeds grown in the environment of the Labinsk district, the Krasnodar Territory, at the premises of Agrofrma Progress, LLC.Materials and methods. To determine factors that affect the spread of thelaziosis, a clinical examination of the Aberdeen Angus cattle of different sex and age groups was performed in Agrofrma Progress LLC. Swabs were taken from the conjunctiva of animals with clinical signs of thelaziosis for further microscopic examination. In 2020, 236 cattle were examined including 42 adult animals older than two years, 77 animals at the age of one year and 117 calves from 0 to 6 months. The species of the pathogens was identifed in the study of the conjunctival swabs according to the adult Thelazia morphology (Gorodovich N. M., 1967). To control ectoparasites and prevent thelaziosis, an insectoacaricide from the synthetic pyrethroid group was used. The study was performed on two livestock farms of Agrofrma Progress, LLC located on different terrains: in a temperate steppe zone with a dry climate and artifcial pastures and in a mountainous zone with a humid climate and natural pastures.Results and discussion. For 2019–2020, thelaziosis was recorded in 236 Aberdeen Angus cattle in the Progress agricultural frm of the Labinsk District, the Krasnodar Territory. A pronounced seasonal dynamic was determined; the spread of infection was recorded from the end of May to October; and the infection peak was in August. We observed a clear correlation of the infection with the activity of zoophilic flies, carriers of thelaziosis. The most widespread thelaziosis was observed in young cattle aged up to 6 months, that were kept on livestock sites on flat terrain in a drier climate.
该研究的目的是确定影响克拉斯诺达尔地区拉宾斯克地区环境中生长的牛肉品种中血吸虫病传播率的因素。为了确定影响滴落病传播的因素，在Agrofrma Progress LLC对不同性别和年龄组的阿伯丁安格斯牛进行了临床检查。从有滴落病临床症状的动物的结膜上取拭子进行进一步的显微镜检查。2020年，对236头牛进行了检查，其中包括42头2岁以上的成年牛，77头1岁的牛和117头0至6个月的小牛。病原体的种类是在结膜拭子研究中根据成虫形态确定的(Gorodovich N. M.， 1967)。采用合成拟除虫菊酯类杀螨剂防治体外寄生虫，预防虫病的发生。该研究是在Agrofrma Progress, LLC位于不同地形的两个牲畜农场进行的:在气候干燥的温带草原区和人工牧场，在气候潮湿的山区和自然牧场。结果和讨论。在2019-2020年期间，克拉斯诺达尔地区拉宾斯克地区Progress农业区的236头阿伯丁安格斯牛中记录了该病。确定了明显的季节动态;从5月底到10月记录了感染的传播;感染高峰出现在8月。我们观察到感染与血吸虫病携带者亲动物蝇的活动有明显的相关性。最广泛的血吸虫病是在6个月大的小牛身上观察到的，这些小牛被饲养在气候干燥的平坦地形的牲畜场地上。
{"title":"Factors influencing the intensity of Thelazia sp. infection in cattle in the Labinsk district of the Krasnodar territory","authors":"E. A. Detkova, S. Lutsuk","doi":"10.31016/1998-8435-2022-16-2-154-159","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-154-159","url":null,"abstract":"The purpose of the research is to determine factors affecting the spread rate of thelaziosis in beef breeds grown in the environment of the Labinsk district, the Krasnodar Territory, at the premises of Agrofrma Progress, LLC.Materials and methods. To determine factors that affect the spread of thelaziosis, a clinical examination of the Aberdeen Angus cattle of different sex and age groups was performed in Agrofrma Progress LLC. Swabs were taken from the conjunctiva of animals with clinical signs of thelaziosis for further microscopic examination. In 2020, 236 cattle were examined including 42 adult animals older than two years, 77 animals at the age of one year and 117 calves from 0 to 6 months. The species of the pathogens was identifed in the study of the conjunctival swabs according to the adult Thelazia morphology (Gorodovich N. M., 1967). To control ectoparasites and prevent thelaziosis, an insectoacaricide from the synthetic pyrethroid group was used. The study was performed on two livestock farms of Agrofrma Progress, LLC located on different terrains: in a temperate steppe zone with a dry climate and artifcial pastures and in a mountainous zone with a humid climate and natural pastures.Results and discussion. For 2019–2020, thelaziosis was recorded in 236 Aberdeen Angus cattle in the Progress agricultural frm of the Labinsk District, the Krasnodar Territory. A pronounced seasonal dynamic was determined; the spread of infection was recorded from the end of May to October; and the infection peak was in August. We observed a clear correlation of the infection with the activity of zoophilic flies, carriers of thelaziosis. The most widespread thelaziosis was observed in young cattle aged up to 6 months, that were kept on livestock sites on flat terrain in a drier climate.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44150644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.31016/1998-8435-2022-16-2-137-146
Yu. A. Prisniy, M. I. Kononova
The purpose of the research is to identify the species composition of helminths in lake frogs (Pelophylax ridibundus (Pallas, 1771)) caught in rivers of the Belgorod Region.Materials and methods. We studied frogs collected using a hydrobiological net in the rivers in fve Belgorod Region districts during the spring-summer 2016–2019. Species identifcation was performed according to morphological characteristics. The sex of the studied frogs was determined by gonads, and the age was determined based on size data. To assess infection of P. ridibundus with individual helminth species and their distribution, we used standard parameters for parasitological survey, namely, the prevalence, the amplitude of infection intensity, and the abundance index.Results and discussion. As a result of helminthological studies of 122 lake frogs, 17 helminth species were identifed which were widely specifc among representatives of the Ranidae family. Presumably, the identifed species form the basis of the species composition of frog helminths in rivers of the Region. Most of these types of helminths occur throughout the springsummer season without pronounced seasonal dynamics. It was noted that the number of helminth species simultaneously parasitizing in one frog increased with age. Opisthioglyphe ranae is the most often numerically dominant species in the examined areas of the Belgorod Region; and Diplodiscus subclavatus, Pleurogenes claviger and Codonocephalus urnigerus mtc have high abundance at some stations.
{"title":"Species composition of helminths in lake frogs Pelophylax ridibundus (Pallas, 1771) in rivers of the Belgorod Region","authors":"Yu. A. Prisniy, M. I. Kononova","doi":"10.31016/1998-8435-2022-16-2-137-146","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-137-146","url":null,"abstract":"The purpose of the research is to identify the species composition of helminths in lake frogs (Pelophylax ridibundus (Pallas, 1771)) caught in rivers of the Belgorod Region.Materials and methods. We studied frogs collected using a hydrobiological net in the rivers in fve Belgorod Region districts during the spring-summer 2016–2019. Species identifcation was performed according to morphological characteristics. The sex of the studied frogs was determined by gonads, and the age was determined based on size data. To assess infection of P. ridibundus with individual helminth species and their distribution, we used standard parameters for parasitological survey, namely, the prevalence, the amplitude of infection intensity, and the abundance index.Results and discussion. As a result of helminthological studies of 122 lake frogs, 17 helminth species were identifed which were widely specifc among representatives of the Ranidae family. Presumably, the identifed species form the basis of the species composition of frog helminths in rivers of the Region. Most of these types of helminths occur throughout the springsummer season without pronounced seasonal dynamics. It was noted that the number of helminth species simultaneously parasitizing in one frog increased with age. Opisthioglyphe ranae is the most often numerically dominant species in the examined areas of the Belgorod Region; and Diplodiscus subclavatus, Pleurogenes claviger and Codonocephalus urnigerus mtc have high abundance at some stations.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47860151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.31016/1998-8435-2022-16-2-177-184
T. E. Pomytkina, O. Bibik, O. I. Pivovar
The purpose of the research is identifying and determining clinical and laboratory features of coinfection caused by Opisthorchis felineus and Lamblia intestinalis.Materials and methods. We described a clinical picture and laboratory abnormalities in 50 patients with O. felineus infection. Of these cases, 25 people were diagnosed with lambliosis. The therapy included three consecutive stages: preparatory stage, antiprotozoal and anthelmintic treatments, and rehabilitation stage. The preparatory stage was aimed to improve the biliary tract function, to reduce intoxication symptoms, and to stop acute allergic reactions. The patients received choleretics, antispasmodics, antibacterial drugs, antihistamines, enzymes, and adsorbents for 2–4 weeks. At the end of the preparatory stage, the patients from the second group were prescribed albendazole of 800 mg per day in two doses of 400 mg per os during meals for 5 days. In the second treatment stage, the patients with opisthorchosis received praziquantel at a dose of 75 mg/kg of body weight per os for one day in three doses. The rehabilitation phase lasted for four months. The next step was to assess lamblia eradication results by the control coproprotozooscopy and/or biliprotozooscopy for cysts and/or vegetative forms of L. intestinalis at 7 days, 1 month and 6 months after treatment with albendazole. The control coproovoscopy for O. felineus eggs was performed at 6 months after praziquantel treatment.Results and discussion. We studied the influence of L. intestinalis on the nature and severity of clinical and laboratory symptoms of opisthorchosis. It was revealed that clinical symptoms (abdominal pain, nausea, low-grade fever or dermatosis) and laboratory syndromes (cytolysis and cholestasis) were more pronounced in case of concomitant helminth and protozoan infections. Lambliosis is more difcult to treat when combined with opisthorchosis.
{"title":"Features of the combined course of opisthorchosis and lambliosis","authors":"T. E. Pomytkina, O. Bibik, O. I. Pivovar","doi":"10.31016/1998-8435-2022-16-2-177-184","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-177-184","url":null,"abstract":"The purpose of the research is identifying and determining clinical and laboratory features of coinfection caused by Opisthorchis felineus and Lamblia intestinalis.Materials and methods. We described a clinical picture and laboratory abnormalities in 50 patients with O. felineus infection. Of these cases, 25 people were diagnosed with lambliosis. The therapy included three consecutive stages: preparatory stage, antiprotozoal and anthelmintic treatments, and rehabilitation stage. The preparatory stage was aimed to improve the biliary tract function, to reduce intoxication symptoms, and to stop acute allergic reactions. The patients received choleretics, antispasmodics, antibacterial drugs, antihistamines, enzymes, and adsorbents for 2–4 weeks. At the end of the preparatory stage, the patients from the second group were prescribed albendazole of 800 mg per day in two doses of 400 mg per os during meals for 5 days. In the second treatment stage, the patients with opisthorchosis received praziquantel at a dose of 75 mg/kg of body weight per os for one day in three doses. The rehabilitation phase lasted for four months. The next step was to assess lamblia eradication results by the control coproprotozooscopy and/or biliprotozooscopy for cysts and/or vegetative forms of L. intestinalis at 7 days, 1 month and 6 months after treatment with albendazole. The control coproovoscopy for O. felineus eggs was performed at 6 months after praziquantel treatment.Results and discussion. We studied the influence of L. intestinalis on the nature and severity of clinical and laboratory symptoms of opisthorchosis. It was revealed that clinical symptoms (abdominal pain, nausea, low-grade fever or dermatosis) and laboratory syndromes (cytolysis and cholestasis) were more pronounced in case of concomitant helminth and protozoan infections. Lambliosis is more difcult to treat when combined with opisthorchosis.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43564083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-16DOI: 10.31016/1998-8435-2022-16-1-112-118
A. I. Varlamova, N. B. Emelyanova, I. Arkhipov, T. S. Novik, K. Kurochkina, V. Abramov
The purpose of the research is to study the embryotropic effect of supramolecular complex of fenbendazole (SMСF).Materials and methods. The experiment to assess the embryotropic properties of SMCF was carried out on 40 white female and 20 male rats in accordance with the Guidelines for the experimental (preclinical) study of new pharmacological substances. Pregnant female rats were divided into 3 experimental and one control groups. SMCF was administered intragastrically on the 1–6 days of embryogenesis (group 1); on the 7–14 days (group 2) and on the 15–19 days (group 3) in three times therapeutic dose – 6,0 mg/kg of active substance. The animals of the control group received saline from the first to the 19th days of pregnancy. Rats were euthanized on the 20th day of pregnancy. The uterus with fetuses was removed after laparotomy, the number of corpora lutea, implantation sites, the number of living, dead and resorbed fetuses were recorded, the weight and diameter of the placenta were determined. The embryos were examined, weighed, the craniocaudal sizes were determined, the levels of total embryonic, preimplantation and postimplantation embryo death were calculated. The fetuses were examined for abnormalities of internal organs and changes in the skeletal system according to the methods of J. G. Wilson (1965) and A. B. Dawson (1926), modified in the department of embryology of the IEM of the USSR Academy of Medical Sciences.Results and discussion. As a result, SMCF does not induce toxic effects on the fetus: mortality rates, size and weight of embryos were at the level of the control group in a threefold therapeutic dose 6,0 mg/kg at intragastric administration on the 1–6; 7–14 and 15–19 days of pregnancy. SMCF did not cause external and internal malformations.
{"title":"Evaluation of supramolecular complex of fenbendazole effect on embryonic development","authors":"A. I. Varlamova, N. B. Emelyanova, I. Arkhipov, T. S. Novik, K. Kurochkina, V. Abramov","doi":"10.31016/1998-8435-2022-16-1-112-118","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-1-112-118","url":null,"abstract":"The purpose of the research is to study the embryotropic effect of supramolecular complex of fenbendazole (SMСF).Materials and methods. The experiment to assess the embryotropic properties of SMCF was carried out on 40 white female and 20 male rats in accordance with the Guidelines for the experimental (preclinical) study of new pharmacological substances. Pregnant female rats were divided into 3 experimental and one control groups. SMCF was administered intragastrically on the 1–6 days of embryogenesis (group 1); on the 7–14 days (group 2) and on the 15–19 days (group 3) in three times therapeutic dose – 6,0 mg/kg of active substance. The animals of the control group received saline from the first to the 19th days of pregnancy. Rats were euthanized on the 20th day of pregnancy. The uterus with fetuses was removed after laparotomy, the number of corpora lutea, implantation sites, the number of living, dead and resorbed fetuses were recorded, the weight and diameter of the placenta were determined. The embryos were examined, weighed, the craniocaudal sizes were determined, the levels of total embryonic, preimplantation and postimplantation embryo death were calculated. The fetuses were examined for abnormalities of internal organs and changes in the skeletal system according to the methods of J. G. Wilson (1965) and A. B. Dawson (1926), modified in the department of embryology of the IEM of the USSR Academy of Medical Sciences.Results and discussion. As a result, SMCF does not induce toxic effects on the fetus: mortality rates, size and weight of embryos were at the level of the control group in a threefold therapeutic dose 6,0 mg/kg at intragastric administration on the 1–6; 7–14 and 15–19 days of pregnancy. SMCF did not cause external and internal malformations.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43083506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-16DOI: 10.31016/1998-8435-2022-16-1-93-100
M. Zubairova, A. Atayev, N. T. Karsakov, M. A. Akhmedov
The purpose of the research is to study mixed infections of gastrointestinal strongylates and Anoplocephala sp. in sheep in the context of the vertical zonality of Dagestan.Materials and methods. In 2000-2020, 360 collections of the digestive tract of sheep and 600 fecal samples were studied in the plain, foothill, and mountain belts of Dagestan. The material was analyzed in young animals up to 1 year old, animals from 1 year to 2 years old, and animals over three years old. The study was performed in all four seasons. The work used methods of full helminthological dissection according to K. I. Skrjabin, sequential washing of feces with a saturated ammonium nitrate solution according to G. A. Kotelnikov, V. M. Khrenov and Berman-Orlov, as well as larvae cultivation in a thermostat.Results and discussion. Mixed helminth infections were recorded in sheep in all natural and climatic zones of Dagestan. The greatest species diversity, high rates of infection prevalence (IP 15.0–43.3%) and infection intensity (II 16–3860 sp.) were observed at gastrointestinal Strongylata and Anoplocephalata infections in the plain belt. Of the pathogens in the plain and foothill belts, mixed infections were dominated by Chabertia, Bunostoma, Trichostrongylidae, Haemonchus, and Nematodirus; and from Anoplocephala – moniezia. The IP and II rates were significantly lower, 25–8.3% and 43–32 sp., respectively, in the mountain belt at an altitude of over 2500 m above the sea level. Lambs in the first year of age were infected with Chabertia, Bunostoma (B. trigonocephalum), Trichostrongylidae (T. axei and T. vitrinus), Haemonchus and Nematodirus (N. spathiger) in the plain and foothill belts. In the second year of age, sheep older than three years were infected with T. axei, T. capricola, T. vitrinus, T. colubriformis, O. ostertagi, O. circumcincta, O. occidentalis, O. antipini, O. leptospicularis, C. punctata, C. oncophora, N. filicollis and N. abnormalis with IP of 2.5–8.3% and II of 1–11 sp., in addition to the dominant species. We recorded M. expansa, M. benedeni, T. giardia and A. centripunctata (IP 2.5–30.0%, and II 1–48 sp.). In the mountain belt over 2500 m above the sea level, lambs were infected with Ch. ovina, B. trigonocephalum, T. axei, H. contortus, N. spathiger (IP 8.3–11.6%, II 3–32 sp.) and M. expansa (IP 2.5–5.0%, II 1–29 sp.). Young animals in the second year of age and sheep over three years old had mixed infections with the species as indicated in lambs (IP 3.5–8.3%, II 3–29 sp.).
本研究的目的是在达吉斯坦垂直地带性的背景下，研究绵羊胃肠道strongylates和Anoplocephala sp.的混合感染。材料和方法。2000-2020年，在达吉斯坦平原、山麓和山区采集了360只羊的消化道标本和600只羊的粪便标本。这些物质在1岁以下的幼龄动物、1岁至2岁的动物和3岁以上的动物中进行了分析。这项研究是在四个季节进行的。根据K. I. Skrjabin的说法，这项工作使用了全面的蠕虫解剖方法，根据G. a . Kotelnikov, V. M. Khrenov和Berman-Orlov的说法，用饱和硝酸铵溶液连续洗涤粪便，以及在恒温器中培养幼虫。结果和讨论。在达吉斯坦所有自然和气候带的绵羊中记录了混合寄生虫感染。平原区胃肠道类圆孔虫和天头虫感染种类多样性最高，感染率最高(15.0 ~ 43.3%)，感染强度最高(II 16 ~ 3860 sp.)。平原和山麓带病原菌以沙伯氏菌、布氏菌、毛圆虫科、血螨和线虫属为主;和从无头蝇- moniezia。在海拔2500 m以上的山地带，IP和II的速率分别为25-8.3%和43 - 32sp .，显著降低。在平原和山麓地带，1岁羔羊感染的主要是Chabertia、Bunostoma (B. trigonocephalum)、trichohostrongylidae (T. axei和T. vitrinus)、Haemonchus和nematovirus (N. spathiger)。2岁时，除优势种外，3岁以上的绵羊还感染了轴突绦虫、山羊绦虫、玻璃状绦虫、彩色绦虫、ostertagi、割皮绦虫、occidentalis、antipini、细钩绦虫、点状绦虫、癌棘绦虫、丝状绦虫和异常绦虫，感染率为2.5 ~ 8.3%，感染率为1 ~ 11 sp.。录得扩张田鼠、贝氏田鼠、贾第弓形虫和向心刺田鼠(ip2.5 ~ 30.0%， ip1 ~ 48sp .)。在海拔2500 m以上的山地地带，羔羊感染的主要是羊蠓、三角头小蠊、轴突小蠊、弓形小蠊、吸血小蠊(ip8.3 ~ 11.6%， i3 ~ 32sp .)和扩张小蠊(ip2.5 ~ 5.0%， i1 ~ 29sp .)。2岁以下的幼畜和3岁以上的绵羊与该物种混合感染，如羔羊(IP为3.5-8.3%，II为3-29 sp.)。
{"title":"Mixed gastrointestinal strongylatosis and anoplocephalatosis of sheep in the context of the vertical zonality of Dagestan","authors":"M. Zubairova, A. Atayev, N. T. Karsakov, M. A. Akhmedov","doi":"10.31016/1998-8435-2022-16-1-93-100","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-1-93-100","url":null,"abstract":"The purpose of the research is to study mixed infections of gastrointestinal strongylates and Anoplocephala sp. in sheep in the context of the vertical zonality of Dagestan.Materials and methods. In 2000-2020, 360 collections of the digestive tract of sheep and 600 fecal samples were studied in the plain, foothill, and mountain belts of Dagestan. The material was analyzed in young animals up to 1 year old, animals from 1 year to 2 years old, and animals over three years old. The study was performed in all four seasons. The work used methods of full helminthological dissection according to K. I. Skrjabin, sequential washing of feces with a saturated ammonium nitrate solution according to G. A. Kotelnikov, V. M. Khrenov and Berman-Orlov, as well as larvae cultivation in a thermostat.Results and discussion. Mixed helminth infections were recorded in sheep in all natural and climatic zones of Dagestan. The greatest species diversity, high rates of infection prevalence (IP 15.0–43.3%) and infection intensity (II 16–3860 sp.) were observed at gastrointestinal Strongylata and Anoplocephalata infections in the plain belt. Of the pathogens in the plain and foothill belts, mixed infections were dominated by Chabertia, Bunostoma, Trichostrongylidae, Haemonchus, and Nematodirus; and from Anoplocephala – moniezia. The IP and II rates were significantly lower, 25–8.3% and 43–32 sp., respectively, in the mountain belt at an altitude of over 2500 m above the sea level. Lambs in the first year of age were infected with Chabertia, Bunostoma (B. trigonocephalum), Trichostrongylidae (T. axei and T. vitrinus), Haemonchus and Nematodirus (N. spathiger) in the plain and foothill belts. In the second year of age, sheep older than three years were infected with T. axei, T. capricola, T. vitrinus, T. colubriformis, O. ostertagi, O. circumcincta, O. occidentalis, O. antipini, O. leptospicularis, C. punctata, C. oncophora, N. filicollis and N. abnormalis with IP of 2.5–8.3% and II of 1–11 sp., in addition to the dominant species. We recorded M. expansa, M. benedeni, T. giardia and A. centripunctata (IP 2.5–30.0%, and II 1–48 sp.). In the mountain belt over 2500 m above the sea level, lambs were infected with Ch. ovina, B. trigonocephalum, T. axei, H. contortus, N. spathiger (IP 8.3–11.6%, II 3–32 sp.) and M. expansa (IP 2.5–5.0%, II 1–29 sp.). Young animals in the second year of age and sheep over three years old had mixed infections with the species as indicated in lambs (IP 3.5–8.3%, II 3–29 sp.).","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46356482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-16DOI: 10.31016/1998-8435-2022-16-1-119-124
S. Egorov, E. Kryuchkova, B. G. Abalikhin, E. A. Sokolov
The purpose of the research is to study the fauna of zoophilous flies on the territory of the farms of the Palekh district of the Ivanovo region, to determine the comparative effectiveness of insecticidal preparations and the duration of their action; establish the role of zoophilous flies in the spread of helminth eggs and protozoan oocysts.Materials and methods. The faunistic collection of zoophilous flies was carried out in calf dispensaries of livestock farms in the farms of the Palekh district of the Ivanovo region. The species of zoophilic flies was determined at the Department of Infectious and Parasitic Diseases of the Ivanovo State Agricultural Academy. The comparative effectiveness of insecticidal preparations was carried out.Results and discussion. The fauna of zoophilic flies on cattle farms in the farms of the Ivanovo region of the Palekh district is represented by three families: Muscidae (92.78%), Calliphoridae (1.16%), Scatophagidae (5.69%). They are equally contaminated with eggs of pathogens of helminthoses and oocysts of pathogens of protozooses, and their role in the spread of helminthosis and protozoosis is determined by the index of dominance in the fauna of livestock buildings. The fastest effect of acute insecticidal action was observed in the drug with AD imidacloprid – 4 min. after contact of insects with treated surfaces of objects. In preparations with AD, thiamethoxam and cypermethrin showed insecticidal properties after 8 min. The longest insecticidal effect was observed in the drug with AD imidacloprid; it was 13 days, while for drugs with DV thiamethoxam and cypermethrin - a little more than half of this period.
{"title":"The role of zoophilous flies in the spread of zoonoses in cattle breeding farms of the Ivanovo region and measures to combat them","authors":"S. Egorov, E. Kryuchkova, B. G. Abalikhin, E. A. Sokolov","doi":"10.31016/1998-8435-2022-16-1-119-124","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-1-119-124","url":null,"abstract":"The purpose of the research is to study the fauna of zoophilous flies on the territory of the farms of the Palekh district of the Ivanovo region, to determine the comparative effectiveness of insecticidal preparations and the duration of their action; establish the role of zoophilous flies in the spread of helminth eggs and protozoan oocysts.Materials and methods. The faunistic collection of zoophilous flies was carried out in calf dispensaries of livestock farms in the farms of the Palekh district of the Ivanovo region. The species of zoophilic flies was determined at the Department of Infectious and Parasitic Diseases of the Ivanovo State Agricultural Academy. The comparative effectiveness of insecticidal preparations was carried out.Results and discussion. The fauna of zoophilic flies on cattle farms in the farms of the Ivanovo region of the Palekh district is represented by three families: Muscidae (92.78%), Calliphoridae (1.16%), Scatophagidae (5.69%). They are equally contaminated with eggs of pathogens of helminthoses and oocysts of pathogens of protozooses, and their role in the spread of helminthosis and protozoosis is determined by the index of dominance in the fauna of livestock buildings. The fastest effect of acute insecticidal action was observed in the drug with AD imidacloprid – 4 min. after contact of insects with treated surfaces of objects. In preparations with AD, thiamethoxam and cypermethrin showed insecticidal properties after 8 min. The longest insecticidal effect was observed in the drug with AD imidacloprid; it was 13 days, while for drugs with DV thiamethoxam and cypermethrin - a little more than half of this period.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45496952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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