Pub Date : 2021-12-19DOI: 10.31016/1998-8435-2021-15-4-76-81
E. Klimova, M. Mkrtchyan, I. Ivanov, T. G. Krylovay
The purpose of the research is studying the spread of cryptosporidiosis in calves and assessment of the extenseeffectiveness of antiprotozoal drugs.Materials and methods. The spread, seasonal and age-specific trends of cryptosporidiosis was studied on 395 spontaneously infected animals of five age groups. The first group was formed of calves up to 10 days of age, the second group of calves aged from 11 days to 2 months, the third group of calves from 2 to 4 months, the fourth group of calves from 4 to 6 months of age, and the fifth group of young animals from 6 months up to 1 year. To assess the efficacy of various drugs against cryptosporidiosis in calves, 5 groups of infected animals (4 experimental and 1 control), 10 animals each, were formed according to the principle of analogous pairs. The infection rate in cattle was determined by generally accepted coprological flotation methods, as well as by native smears with further staining according to Ziehl-Nielsen. We also considered clinical signs of cryptosporidiosis (diarrhea, dehydration).Results and discussion. We established the widespread occurrence of cryptosporidiosis in cattle on the farms of the Igrinsky, Uvinsky, Zavyalovsky Districts of the Udmurt Republic. The infection rate ranged from 20.25 to 80.0%. The largest percentage of infected calves was observed in the winter-spring period which was associated with the synchronization of the reproductive cycles of cows and a decrease in the resistance of the animal organism. Toltarox 5% and Galokur drugs in work environments showed 100% extense-effectiveness. It is not recommended to apply Amprolium 25% on these farms due to the low efficacy and the development of parasite resistance to this drug.
{"title":"Assessment of the therapeutic efficacy of various drugs against cryptosporidiosis in calves","authors":"E. Klimova, M. Mkrtchyan, I. Ivanov, T. G. Krylovay","doi":"10.31016/1998-8435-2021-15-4-76-81","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-76-81","url":null,"abstract":"The purpose of the research is studying the spread of cryptosporidiosis in calves and assessment of the extenseeffectiveness of antiprotozoal drugs.Materials and methods. The spread, seasonal and age-specific trends of cryptosporidiosis was studied on 395 spontaneously infected animals of five age groups. The first group was formed of calves up to 10 days of age, the second group of calves aged from 11 days to 2 months, the third group of calves from 2 to 4 months, the fourth group of calves from 4 to 6 months of age, and the fifth group of young animals from 6 months up to 1 year. To assess the efficacy of various drugs against cryptosporidiosis in calves, 5 groups of infected animals (4 experimental and 1 control), 10 animals each, were formed according to the principle of analogous pairs. The infection rate in cattle was determined by generally accepted coprological flotation methods, as well as by native smears with further staining according to Ziehl-Nielsen. We also considered clinical signs of cryptosporidiosis (diarrhea, dehydration).Results and discussion. We established the widespread occurrence of cryptosporidiosis in cattle on the farms of the Igrinsky, Uvinsky, Zavyalovsky Districts of the Udmurt Republic. The infection rate ranged from 20.25 to 80.0%. The largest percentage of infected calves was observed in the winter-spring period which was associated with the synchronization of the reproductive cycles of cows and a decrease in the resistance of the animal organism. Toltarox 5% and Galokur drugs in work environments showed 100% extense-effectiveness. It is not recommended to apply Amprolium 25% on these farms due to the low efficacy and the development of parasite resistance to this drug.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45764197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-19DOI: 10.31016/1998-8435-2021-15-4-100-105
B. M. Makhieva, J. M. Ozdemirova, A. B. Dagayeva, R. M. Bakrieva
The purpose of the research is efficacy evaluation of the anticoccidial drug Solycox combined with the feed additive Chiktonik against avian eimeriosis.Materials and methods. The experiments were carried out on the Makhachkalinskaya poultry farm, which was contaminated with eimeriosis, on healthy 1 day old Ross-308 broilers. Two groups were formed from the selected chickens, test and control, 50 birds each. From the first day of life, broiler chickens from the test group were administered Solycox at a dose of 2 ml per 1 liter of drinking water combined with the feed additive Chiktonik at the rate of 1 ml per 1 liter of drinking water. The control chickens were not prescribed any anticoccidial drug or feed additive.Results and discussion. We established high efficacy of Solycox combined with Chiktonik against eimeriosis of broiler chickens (96%). Solycox combined with Chiktonik contributes to the poultry viability and resistance to the disease, and has a positive effect on productivity. The results of the studies allow us to recommend Solycox and Chiktonik to prevent and treat eimeriosis of broiler chickens.
{"title":"The efficacy of the drug Solycox combined with Chiktonik against eimeriosis of broiler chickens","authors":"B. M. Makhieva, J. M. Ozdemirova, A. B. Dagayeva, R. M. Bakrieva","doi":"10.31016/1998-8435-2021-15-4-100-105","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-100-105","url":null,"abstract":"The purpose of the research is efficacy evaluation of the anticoccidial drug Solycox combined with the feed additive Chiktonik against avian eimeriosis.Materials and methods. The experiments were carried out on the Makhachkalinskaya poultry farm, which was contaminated with eimeriosis, on healthy 1 day old Ross-308 broilers. Two groups were formed from the selected chickens, test and control, 50 birds each. From the first day of life, broiler chickens from the test group were administered Solycox at a dose of 2 ml per 1 liter of drinking water combined with the feed additive Chiktonik at the rate of 1 ml per 1 liter of drinking water. The control chickens were not prescribed any anticoccidial drug or feed additive.Results and discussion. We established high efficacy of Solycox combined with Chiktonik against eimeriosis of broiler chickens (96%). Solycox combined with Chiktonik contributes to the poultry viability and resistance to the disease, and has a positive effect on productivity. The results of the studies allow us to recommend Solycox and Chiktonik to prevent and treat eimeriosis of broiler chickens.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47583645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-19DOI: 10.31016/1998-8435-2021-15-4-36-42
M. Zubairova, A. M. Atayev , N. T. Karsakov, Z. Dzhambulatov, S. Atayeva
The purpose of the research is studying the oribatid mite biodiversity on the Dagestan pastures in terms of altitudinal zonation and their infection with Moniezia sp. procercoids.Materials and methods. Oribatid mites were collected in different seasons of 1990–2020 on different types of pastures of the plain, foothill, and mountain belts of Dagestan. A total of 16,000 specimens of oribatid mites were collected. 120 sets of lamb intestines were dissected. Oribatid mites were collected using the Tulgren funnel. The method of complete helminthological dissection according to K. I. Skrjabin was used in the work.Results and discussion. On low-lying wet pastures of the flat belt, up to 5800 oribatid specimens were recorded per 1 m² with 38.0% prevalence of infection (PI) with moniezia cysticercoids; 675 specimens were collected on the steppe lands with the PI of 12.5%, up to 140 specimens were collected on salt marshes with the PI 0.9%, and 52 specimens were collected in the semi-deserts with the PI 0.4%. In the foothill steppes, 1,300 oribatids specimens were found per 1 m² with their procercoid infeсtion up to 18.0%, 2100 specimens with 16.0% were collected along river valleys, and 120 specimens with the PI of 0.5% on mountain plateaus. Lambs on low-lying wetlands of the lowland belt were infected with Moniezia sp. by 72.0% with the infection intensity (II) of 8-116 specimens, on steppe pastures by 67.5% at the II of 5–36 specimens, on salt marshes by 18.0% with the II of 2–8 specimens, and in semi-deserts by 12.0% with the II of 2–5 specimens. In the foothill steppes, lambs were infected with Moniezia sp. by 68.0% with the II of 9–64 specimens, along river valleys by 69.0% with the II of 11–62 specimens, and on mountain plateaus by 12.0% with the II of 2–4 specimens.
{"title":"Biodiversity of oribatid mites in the ecosystems of Dagestan and their infection with Moniezia sp. procercoids","authors":"M. Zubairova, A. M. Atayev , N. T. Karsakov, Z. Dzhambulatov, S. Atayeva","doi":"10.31016/1998-8435-2021-15-4-36-42","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-36-42","url":null,"abstract":"The purpose of the research is studying the oribatid mite biodiversity on the Dagestan pastures in terms of altitudinal zonation and their infection with Moniezia sp. procercoids.Materials and methods. Oribatid mites were collected in different seasons of 1990–2020 on different types of pastures of the plain, foothill, and mountain belts of Dagestan. A total of 16,000 specimens of oribatid mites were collected. 120 sets of lamb intestines were dissected. Oribatid mites were collected using the Tulgren funnel. The method of complete helminthological dissection according to K. I. Skrjabin was used in the work.Results and discussion. On low-lying wet pastures of the flat belt, up to 5800 oribatid specimens were recorded per 1 m² with 38.0% prevalence of infection (PI) with moniezia cysticercoids; 675 specimens were collected on the steppe lands with the PI of 12.5%, up to 140 specimens were collected on salt marshes with the PI 0.9%, and 52 specimens were collected in the semi-deserts with the PI 0.4%. In the foothill steppes, 1,300 oribatids specimens were found per 1 m² with their procercoid infeсtion up to 18.0%, 2100 specimens with 16.0% were collected along river valleys, and 120 specimens with the PI of 0.5% on mountain plateaus. Lambs on low-lying wetlands of the lowland belt were infected with Moniezia sp. by 72.0% with the infection intensity (II) of 8-116 specimens, on steppe pastures by 67.5% at the II of 5–36 specimens, on salt marshes by 18.0% with the II of 2–8 specimens, and in semi-deserts by 12.0% with the II of 2–5 specimens. In the foothill steppes, lambs were infected with Moniezia sp. by 68.0% with the II of 9–64 specimens, along river valleys by 69.0% with the II of 11–62 specimens, and on mountain plateaus by 12.0% with the II of 2–4 specimens.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48042527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-19DOI: 10.31016/1998-8435-2021-15-4-82-90
A. Konovalov, I. I. Tsepilova, F. Vasilevich
The purpose of the research is evaluating the efficacy of complex therapy using dironet, lactobifadol and keratin food supplement (DLK) against toxascariosis of the arctic fox.Materials and methods. To determine the helminth fauna in the conditions of Vyatka Fur Breeding Farm, 61 females silver fox and 55 females voilevoy arctic fox were used as study objects. To conduct complex therapy against toxascariosis, the study object was 24 females culled from the breeding herd, spontaneously infected with toxascariosis and being clinically healthy. The morphological and biochemical blood parameters in female foxes were determined before and after dehelminthization, and control weighing of animals from experimental groups was carried out before and after the experiment.Results and discussion. Of the studied 116 fur-bearing animals, 26 (22.4%) were infected with Toxascaris leonina. Infection rate of Toxascaris sp. in silver fox females aged 5–7 years was 42.6% when 7-9 eggs were found in one microscope field (magnification 7 × 9). Infection with Toxascaris sp. affects on the body weight of infected animals. The females silver fox from the second experimental group infected with Toxascaris sp., which were not treated, had an average live weight of 1.3 kg less (16.6%) as compared to the control. The complex therapy with the DLK treatment-and-prophylactic complex contributed to the improved metabolic processes and digestion. The applicability of the DLK treatment-and-prophylactic complex for therapy against toxascariosis was proved.
{"title":"Complex therapy against toxascariosis of caged silver foxes","authors":"A. Konovalov, I. I. Tsepilova, F. Vasilevich","doi":"10.31016/1998-8435-2021-15-4-82-90","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-82-90","url":null,"abstract":"The purpose of the research is evaluating the efficacy of complex therapy using dironet, lactobifadol and keratin food supplement (DLK) against toxascariosis of the arctic fox.Materials and methods. To determine the helminth fauna in the conditions of Vyatka Fur Breeding Farm, 61 females silver fox and 55 females voilevoy arctic fox were used as study objects. To conduct complex therapy against toxascariosis, the study object was 24 females culled from the breeding herd, spontaneously infected with toxascariosis and being clinically healthy. The morphological and biochemical blood parameters in female foxes were determined before and after dehelminthization, and control weighing of animals from experimental groups was carried out before and after the experiment.Results and discussion. Of the studied 116 fur-bearing animals, 26 (22.4%) were infected with Toxascaris leonina. Infection rate of Toxascaris sp. in silver fox females aged 5–7 years was 42.6% when 7-9 eggs were found in one microscope field (magnification 7 × 9). Infection with Toxascaris sp. affects on the body weight of infected animals. The females silver fox from the second experimental group infected with Toxascaris sp., which were not treated, had an average live weight of 1.3 kg less (16.6%) as compared to the control. The complex therapy with the DLK treatment-and-prophylactic complex contributed to the improved metabolic processes and digestion. The applicability of the DLK treatment-and-prophylactic complex for therapy against toxascariosis was proved.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42674028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-19DOI: 10.31016/1998-8435-2021-15-4-51-56
R. M. Bakrieva
The purpose of the research is studying therapeutic properties of Butakur against spontaneous bovine theileriosis.Materials and methods. Butakur was used on the Yalgin Farm and at the Umarov Agricultural Enterprise in the Karabudakhkentskiy District to treat 30 animals in the early stage of the disease at a body temperature of 40.4–41.7 0C and a parasitic reaction of 30.2%. Piroplasmids were differentiated by microscopy of smears, imprints of lymph nodes and peripheral blood, and by detected purple bodies and parasites in them, excluding mixed infection. Two groups of animals were formed on the principle of analogs. In the test group (n = 15), Butakur was used as a specific therapy at a dose of 2.5 mg/kg for the active substance at a rate of 1 ml/20 kg of body weight, and the antimalarial drug Delagil (chloroquine) was used in the control group at a dose of 15 mg/kg within 4–5 days. A different combination of chemotherapy drugs with symptom-directed and pathogenetic drugs was used simultaneously.Results and discussion. Butakur was the most effective when used in the early period of the disease development from the moment the body temperature rose and until a slight parasitemia in erythrocytes was observed with the simultaneous use of symptom-directed and pathogenetic drugs. Out of 15 animals that received Butakur at a dose of 1 ml/20 kg (2.5 mg/kg for the active substance), 13 animals recovered, and the efficacy was 86.6%.
{"title":"Therapeutic efficacy of Butakur against bovine theileriosis in the Caspian Sea Region of Russia","authors":"R. M. Bakrieva","doi":"10.31016/1998-8435-2021-15-4-51-56","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-51-56","url":null,"abstract":"The purpose of the research is studying therapeutic properties of Butakur against spontaneous bovine theileriosis.Materials and methods. Butakur was used on the Yalgin Farm and at the Umarov Agricultural Enterprise in the Karabudakhkentskiy District to treat 30 animals in the early stage of the disease at a body temperature of 40.4–41.7 0C and a parasitic reaction of 30.2%. Piroplasmids were differentiated by microscopy of smears, imprints of lymph nodes and peripheral blood, and by detected purple bodies and parasites in them, excluding mixed infection. Two groups of animals were formed on the principle of analogs. In the test group (n = 15), Butakur was used as a specific therapy at a dose of 2.5 mg/kg for the active substance at a rate of 1 ml/20 kg of body weight, and the antimalarial drug Delagil (chloroquine) was used in the control group at a dose of 15 mg/kg within 4–5 days. A different combination of chemotherapy drugs with symptom-directed and pathogenetic drugs was used simultaneously.Results and discussion. Butakur was the most effective when used in the early period of the disease development from the moment the body temperature rose and until a slight parasitemia in erythrocytes was observed with the simultaneous use of symptom-directed and pathogenetic drugs. Out of 15 animals that received Butakur at a dose of 1 ml/20 kg (2.5 mg/kg for the active substance), 13 animals recovered, and the efficacy was 86.6%.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48804443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-19DOI: 10.31016/1998-8435-2021-15-4-43-50
A. I. Varlamova, V. Abramov, I. Arkhipov
The purpose of the research is to develop a methodology for the quantitative determination of fenbendazole (FBZ) and its metabolites in organs and tissues of sheep by high performance liquid chromatography tandem mass-spectrometry (HPLC-MS/MS).Materials and methods. The method for determining of FBZ and its metabolites after the administration of the supramolecular complex of fenbendazole was validated in accordance with international guidelines for the following indicators: linearity, recovery, specificity, precision, accuracy, limits of quantitative and qualitative determination.Results and discussion. The method of quantitative analysis of FBZ and its metabolites sulfone and sulfoxide in the body of sheep after the administration of the supramolecular complex has been adapted. The proposed method has a linear relationship (R > 0.99) in the range of 5–1000 ng / g and has shown a good reproducibility and accuracy.
{"title":"Methodology for the quantitative determination of fenbendazole and its metabolites in organs and tissues of animals by high performance liquid chromatography with tandem mass spectrometry detection","authors":"A. I. Varlamova, V. Abramov, I. Arkhipov","doi":"10.31016/1998-8435-2021-15-4-43-50","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-43-50","url":null,"abstract":"The purpose of the research is to develop a methodology for the quantitative determination of fenbendazole (FBZ) and its metabolites in organs and tissues of sheep by high performance liquid chromatography tandem mass-spectrometry (HPLC-MS/MS).Materials and methods. The method for determining of FBZ and its metabolites after the administration of the supramolecular complex of fenbendazole was validated in accordance with international guidelines for the following indicators: linearity, recovery, specificity, precision, accuracy, limits of quantitative and qualitative determination.Results and discussion. The method of quantitative analysis of FBZ and its metabolites sulfone and sulfoxide in the body of sheep after the administration of the supramolecular complex has been adapted. The proposed method has a linear relationship (R > 0.99) in the range of 5–1000 ng / g and has shown a good reproducibility and accuracy.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46955732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-19DOI: 10.31016/1998-8435-2021-15-4-61-75
николаевна Индюхова, Михаил Владимирович Арисов, Владимир Ильич Максимов, Т. О. Азарнова
The purpose of the research is to study the efficacy of the insectoacaricide "5% D-cyphenotrine emulsion" against infestation of birds with Dermanyssus gallinae (dermanyssosis) after the deacarization of the poultry building and to characterize physiological and biochemical mechanisms of the recovery of Hy-Line hens after removed parasites.Materials and methods. A parasitological examination of the poultry farm found D. gallinae in one of the poultry buildings. Given positive results of the drug efficacy against the poultry red mite, we analyzed the morpho-physiological and physiological and biochemical parameters of the hen’s blood before and 10 and 20 days after the treatments. Changes in the ethological status of birds were recorded. We used 0.005% aqueous emulsion of "5% D-cyphenothrin emulsion" for treatment. To obtain it, the drug was diluted with water at a ratio of 1:1000 immediately before use.Results and discussion. After double treatment with "5% D-cyphenotrine emulsion" of the shop with hens from the test group, no live poultry red mite was found. Within 20 days after the disease reduction, representatives of this group showed high concentrations of cortisol which were 2.3–2.4 times (p < 0.001) higher than those of healthy hens. This indicates that the stress state of hens still remains after the parasitizing of D. gallinae. In addition, the recovery process takes a long time and with great stress for all systems of their body. This is also confirmed by a high level of lipid peroxidation. At the same time, high intensity of gluconeogenesis and, as a result, deterioration of lipid and protein metabolism were recorded in the hens in question. The positive results indicate an increasing need for additional energy expenditures which are paramount for the effective and well-timed implementation of adaptation mechanisms. It should be noted here that the values of all the above-mentioned indicators tend to normalize by the end of the study period. The changes noted in the blood of birds indicate the restoration of a number of body systems. The poultry red mite as an emergency factor affects the morphophysiology of the blood, in particular, the level of white blood cells, which is restored by the 10th day after deacarization, which triggers regenerative and reparative processes. There were also changes in the number of cells of the immune system, an increase in hemoglobin concentration to control values and optimization of behavior in representatives of the test group which are largely related to the normalized physiological activity of the thyroid gland after the poultry building deacarization. We found a partial recovery of the hen’s body systems after the poultry building deacarization with the drug "5% D-cyphenotrine emulsion", which is due to the long-term parasitizing of D. gallinae, a stressor of extreme strength.
本研究旨在研究杀虫剂“5% d -环苯三胺乳剂”对家禽舍除虫后鸡皮病的防治效果，并探讨除虫后海兰鸡恢复的生理生化机制。材料和方法。对该家禽饲养场进行的寄生虫学检查在其中一幢禽舍内发现鸡痢杆菌。鉴于该药对禽红螨的药效呈阳性，我们对用药前、用药后10、20 d的母鸡血液形态生理和生理生化指标进行了分析。记录了鸟类行为学状况的变化。采用0.005%的“5% d -氯氰菊酯乳液”水溶液进行处理。为了获得它，药物在使用前立即以1:1000的比例用水稀释。结果和讨论。试验组母鸡所在店经“5% d -环苯三胺乳剂”双重处理后，未发现活禽红螨。在疾病减少后的20天内，该组代表鸡的皮质醇浓度较高，比健康母鸡高2.3-2.4倍(p < 0.001)。这说明母鸡在被鸡寄生后仍处于应激状态。此外，恢复过程需要很长时间，对他们身体的所有系统都有很大的压力。脂质过氧化水平高也证实了这一点。同时，所研究的母鸡出现了高强度的糖异生，并因此导致脂质和蛋白质代谢的恶化。积极的结果表明，越来越需要额外的能源支出，这对于有效和及时实施适应机制至关重要。在此需要指出的是，上述所有指标的数值在研究期结束时趋于正常化。在鸟类血液中发现的变化表明一些身体系统得到了恢复。家禽红螨作为紧急因子影响血液形态生理，特别是白细胞水平，在去角质后第10天恢复，从而触发再生和修复过程。免疫系统细胞数量的变化、血红蛋白浓度控制值的增加以及试验组代表的行为优化，在很大程度上与家禽舍体去角质后甲状腺生理活动的正常化有关。我们发现，用“5% d -环苯三胺乳剂”药物对家禽进行建筑去角质后，母鸡的身体系统部分恢复，这是由于长期寄生在鸡单胞菌(D. gallinae)中，这是一种强度极高的应激源。
{"title":"Physiological and biochemical mechanisms of the hen’s body recovery from dermanyssosis associated with deacarization","authors":"николаевна Индюхова, Михаил Владимирович Арисов, Владимир Ильич Максимов, Т. О. Азарнова","doi":"10.31016/1998-8435-2021-15-4-61-75","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-61-75","url":null,"abstract":"The purpose of the research is to study the efficacy of the insectoacaricide \"5% D-cyphenotrine emulsion\" against infestation of birds with Dermanyssus gallinae (dermanyssosis) after the deacarization of the poultry building and to characterize physiological and biochemical mechanisms of the recovery of Hy-Line hens after removed parasites.Materials and methods. A parasitological examination of the poultry farm found D. gallinae in one of the poultry buildings. Given positive results of the drug efficacy against the poultry red mite, we analyzed the morpho-physiological and physiological and biochemical parameters of the hen’s blood before and 10 and 20 days after the treatments. Changes in the ethological status of birds were recorded. We used 0.005% aqueous emulsion of \"5% D-cyphenothrin emulsion\" for treatment. To obtain it, the drug was diluted with water at a ratio of 1:1000 immediately before use.Results and discussion. After double treatment with \"5% D-cyphenotrine emulsion\" of the shop with hens from the test group, no live poultry red mite was found. Within 20 days after the disease reduction, representatives of this group showed high concentrations of cortisol which were 2.3–2.4 times (p < 0.001) higher than those of healthy hens. This indicates that the stress state of hens still remains after the parasitizing of D. gallinae. In addition, the recovery process takes a long time and with great stress for all systems of their body. This is also confirmed by a high level of lipid peroxidation. At the same time, high intensity of gluconeogenesis and, as a result, deterioration of lipid and protein metabolism were recorded in the hens in question. The positive results indicate an increasing need for additional energy expenditures which are paramount for the effective and well-timed implementation of adaptation mechanisms. It should be noted here that the values of all the above-mentioned indicators tend to normalize by the end of the study period. The changes noted in the blood of birds indicate the restoration of a number of body systems. The poultry red mite as an emergency factor affects the morphophysiology of the blood, in particular, the level of white blood cells, which is restored by the 10th day after deacarization, which triggers regenerative and reparative processes. There were also changes in the number of cells of the immune system, an increase in hemoglobin concentration to control values and optimization of behavior in representatives of the test group which are largely related to the normalized physiological activity of the thyroid gland after the poultry building deacarization. We found a partial recovery of the hen’s body systems after the poultry building deacarization with the drug \"5% D-cyphenotrine emulsion\", which is due to the long-term parasitizing of D. gallinae, a stressor of extreme strength.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43982240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-18DOI: 10.31016/1998-8435-2021-15-4-22-28
A. Atayev, M. Zubairova, N. T. Karsakov
The purpose of the research is study of some reproductive characteristics of the biology of ticks of the family Ixodidae which are widespread in the southeast of the North Caucasus.Materials and methods. In 2000–2010, 9 series of experiments were carried out in the conditions of the plain belt in Dagestan. The first, second, and third series of experiments were intended to find out the duration of the tick searching for a place to fix on the animal, the duration of the skin incised and the proboscis inserted into the wound, and the engorgement duration; and the fourth series was intended to clarify the tick wintering on the animal. Such experiments were performed on two-year-old calf bulls on the Educational and Experimental Farm of the Dagestan State Agrarian University with Boophilus annulatus, Rhipicephalus bursa, Hyalomma detritum, H. scupense, and H. anatolicum in 2000. Five hungry adult female ticks were placed onto all 5 calf bulls in the middle third of the neck. We conducted experiments on searching for a place for egg-laying, egg-laying duration in the environment, number of eggs in a clutch, duration of the larva formed and the hatching rate, distance of the larvae from the hatching place, and larvae death rate in five specially equipped sites of pastures of 1 m2 . Five adult female ticks of B. annulatus, R. bursa, H. detritum, H. scupense, and H. anatolicum were placed after engorgement in each biological site.Results and discussion. Ixodid ticks perform their natural physiological functions associated with feeding and reproduction with significant time fluctuations. The search for a place to fix on the animal took 4–8 hours; the skin incised and proboscis inserted in the wound took 2–5 hours; the engorgement took 4–6 hours; the search for an egg-laying place took 10– 22 hours; the egg-laying duration in the environment was 18–23 hours; the number of eggs in a clutch was 4–13K; the duration of the larva formed and the hatching rate was 10–15 days and 58.0–75% respectively; the distance of the larva from the hatching place was 0.5–1.5 m; death rate of the larva in captivity was 100%; and the tick wintering on the animal was near the neck, ears, in the dewlap, the flank and the udder. These features are typical for the analyzed tick species in the southeast of the North Caucasus and, probably, for other taxa of these genera.
{"title":"Reproductive features of the biology of some ticks of the family Ixodidae Murray, 1877 widespread in the southeast of the North Caucasus","authors":"A. Atayev, M. Zubairova, N. T. Karsakov","doi":"10.31016/1998-8435-2021-15-4-22-28","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-22-28","url":null,"abstract":"The purpose of the research is study of some reproductive characteristics of the biology of ticks of the family Ixodidae which are widespread in the southeast of the North Caucasus.Materials and methods. In 2000–2010, 9 series of experiments were carried out in the conditions of the plain belt in Dagestan. The first, second, and third series of experiments were intended to find out the duration of the tick searching for a place to fix on the animal, the duration of the skin incised and the proboscis inserted into the wound, and the engorgement duration; and the fourth series was intended to clarify the tick wintering on the animal. Such experiments were performed on two-year-old calf bulls on the Educational and Experimental Farm of the Dagestan State Agrarian University with Boophilus annulatus, Rhipicephalus bursa, Hyalomma detritum, H. scupense, and H. anatolicum in 2000. Five hungry adult female ticks were placed onto all 5 calf bulls in the middle third of the neck. We conducted experiments on searching for a place for egg-laying, egg-laying duration in the environment, number of eggs in a clutch, duration of the larva formed and the hatching rate, distance of the larvae from the hatching place, and larvae death rate in five specially equipped sites of pastures of 1 m2 . Five adult female ticks of B. annulatus, R. bursa, H. detritum, H. scupense, and H. anatolicum were placed after engorgement in each biological site.Results and discussion. Ixodid ticks perform their natural physiological functions associated with feeding and reproduction with significant time fluctuations. The search for a place to fix on the animal took 4–8 hours; the skin incised and proboscis inserted in the wound took 2–5 hours; the engorgement took 4–6 hours; the search for an egg-laying place took 10– 22 hours; the egg-laying duration in the environment was 18–23 hours; the number of eggs in a clutch was 4–13K; the duration of the larva formed and the hatching rate was 10–15 days and 58.0–75% respectively; the distance of the larva from the hatching place was 0.5–1.5 m; death rate of the larva in captivity was 100%; and the tick wintering on the animal was near the neck, ears, in the dewlap, the flank and the udder. These features are typical for the analyzed tick species in the southeast of the North Caucasus and, probably, for other taxa of these genera.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45789343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-18DOI: 10.31016/1998-8435-2021-15-4-11-21
O. Davydova, N. Esaulova, Natalia Kryukova
The purpose of the research is study of pinniped helminth fauna in Chukotka, and the analysis of the fish of the main commercial families infected with pathogens of helminthozoonoses based on modern literature.Materials and methods. The helminths were collected in autumn of 2019 from pinnipeds caught in the Mechigmenskaya Guba of the Bering Sea in the Chukotka Autonomous Okrug by the method of partial helminthological dissection per Skryabin (gastrointestinal tract). Samples were examined from 6 walruses and 26 seals (13 spotted seals and 13 ringed seals). The helminths found were fixed in 70% alcohol. The helminth species were identified at the Department of Parasitology and Veterinary and Sanitary Examination of the MVA named after K. I. Skryabin using reference literature.Results and discussion. All pinnipeds were infected with nematodes of the family Anisakidae. Mature Pseudoterranova desipiens were found in the walrus (Infection Prevalence = 16.7% with Infection Intensity = 3 specimens/animal), mature Ps. desipiens, as well as Contracoecum osculatum and Anisakis simplex larvae (IP = 30.8% with II from 5 to 57 specimens) were found in the spotted seal, and Ps. desipiens larvae and mature Ps. desipiens were found in the ringed seal (IP = 15.4% with II from 1 to 4 specimens). Thus, only Ps. desipiens were represented by mature stages (females and males), and two other species of anisakids, C. osculatum and A. simplex, were found in the seals in the larval stage.
本研究的目的是研究楚科奇地区的鳍足蠕虫区系，并在现代文献的基础上分析主要商业家庭的鱼类感染蠕虫人畜共患病病原体。材料和方法。这些蠕虫是于2019年秋季从楚科奇自治区白令海的Mechigmenskaya Guba捕获的鳍足类动物中采集的，方法是对Skryabin(胃肠道)进行部分蠕虫解剖。对6只海象和26只海豹(13只斑海豹和13只环斑海豹)进行了样本检测。发现的蠕虫被固定在70%的酒精中。寄生虫种类是在以K. I. Skryabin命名的MVA寄生虫学和兽医卫生检验科根据参考文献鉴定的。结果和讨论。所有鳍足动物均感染了八角虫科线虫。海象中检出成熟的拟陆地绦虫(感染率为16.7%，感染强度为3只/只)，斑海豹中检出成熟的拟陆地绦虫，并检出鳞状收缩绦虫和单纯异尖线虫幼虫(感染率为30.8%，感染ⅱ型)，斑海豹中检出成熟的拟陆地绦虫幼虫和成熟的拟陆地绦虫(感染率为15.4%，感染ⅱ型)。因此，在海豹的成熟期(雌性和雄性)中，只发现了长纹姬纹姬蚊，而在幼龄阶段，在海豹体内发现了2种不同种类的异色姬蚊和单形姬蚊。
{"title":"Loimologically significant pinniped helminths in Chukotka","authors":"O. Davydova, N. Esaulova, Natalia Kryukova","doi":"10.31016/1998-8435-2021-15-4-11-21","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-4-11-21","url":null,"abstract":"The purpose of the research is study of pinniped helminth fauna in Chukotka, and the analysis of the fish of the main commercial families infected with pathogens of helminthozoonoses based on modern literature.Materials and methods. The helminths were collected in autumn of 2019 from pinnipeds caught in the Mechigmenskaya Guba of the Bering Sea in the Chukotka Autonomous Okrug by the method of partial helminthological dissection per Skryabin (gastrointestinal tract). Samples were examined from 6 walruses and 26 seals (13 spotted seals and 13 ringed seals). The helminths found were fixed in 70% alcohol. The helminth species were identified at the Department of Parasitology and Veterinary and Sanitary Examination of the MVA named after K. I. Skryabin using reference literature.Results and discussion. All pinnipeds were infected with nematodes of the family Anisakidae. Mature Pseudoterranova desipiens were found in the walrus (Infection Prevalence = 16.7% with Infection Intensity = 3 specimens/animal), mature Ps. desipiens, as well as Contracoecum osculatum and Anisakis simplex larvae (IP = 30.8% with II from 5 to 57 specimens) were found in the spotted seal, and Ps. desipiens larvae and mature Ps. desipiens were found in the ringed seal (IP = 15.4% with II from 1 to 4 specimens). Thus, only Ps. desipiens were represented by mature stages (females and males), and two other species of anisakids, C. osculatum and A. simplex, were found in the seals in the larval stage.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45590078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-15DOI: 10.31016/1998-8435-2021-15-3-32-47
N. Romanova, N. Golovina, A. Vishtorskaya, P. Golovin
The purpose of the research is determination of the species composition of parasites of cyprinid and percoid fish in the reservoirs of the Moscow Canal.Materials and methods. In 2019–2020, parasitological material was collected in the spring, summer and autumn from percoid (the pike perch and European perch) and cyprinid fish (the bream, silver bream, roach, rudd and sabrefish) aged 2 to 7 years from the reservoirs of the Moscow Canal (Ikshinsky, Pestovsky, Pyalovsky and Klyazminsky Reservoirs) by the methods generally accepted in ichthyo-parasitology. To quantify the fish infection rate, we used the incidence or prevalence of infection, the intensity of infection, and the mean amplitude of the intensity of infection. We used Kabiosh index (K) to assess the species diversity of parasites. Results and discussion. The parasitological analysis results of percoids and cyprinids from the reservoirs of the Moscow Canal are presented. 34 parasite species were found in fish, which belong to nine taxonomic groups: Microsporidia, Myxosporea, Monogenea, Cestoda, Trematoda, Nematoda, Acanthocephala, Hirudinea, Crustacea. The largest number of species belongs to trematodes, the rest, taxa (mixo- and microsporidia, cestodes, nematodes, acanthocephalans, leeches and parasitic crustaceans), are represented singly. We calculated the species diversity ratio (R) of fish parasites in the reservoirs, which ranged from 0.335 to 0.575, which indicates the fauna similarity from 50 to 75%. The greatest similarity in the parasite fauna was found in the roach (R = 0.168–0.447), the least in the European perch (R = 0.447–1.0). We have identified the dominant species that are found in more than 20% of the studied fish and determine the “core” of the parasitic fauna, which included 4 parasite species of epizootic and 1 parasite species of epidemiological significance.
{"title":"Fauna of parasite of cyprinids and percoids in the reservoirs of the Moscow Canal","authors":"N. Romanova, N. Golovina, A. Vishtorskaya, P. Golovin","doi":"10.31016/1998-8435-2021-15-3-32-47","DOIUrl":"https://doi.org/10.31016/1998-8435-2021-15-3-32-47","url":null,"abstract":"The purpose of the research is determination of the species composition of parasites of cyprinid and percoid fish in the reservoirs of the Moscow Canal.Materials and methods. In 2019–2020, parasitological material was collected in the spring, summer and autumn from percoid (the pike perch and European perch) and cyprinid fish (the bream, silver bream, roach, rudd and sabrefish) aged 2 to 7 years from the reservoirs of the Moscow Canal (Ikshinsky, Pestovsky, Pyalovsky and Klyazminsky Reservoirs) by the methods generally accepted in ichthyo-parasitology. To quantify the fish infection rate, we used the incidence or prevalence of infection, the intensity of infection, and the mean amplitude of the intensity of infection. We used Kabiosh index (K) to assess the species diversity of parasites. Results and discussion. The parasitological analysis results of percoids and cyprinids from the reservoirs of the Moscow Canal are presented. 34 parasite species were found in fish, which belong to nine taxonomic groups: Microsporidia, Myxosporea, Monogenea, Cestoda, Trematoda, Nematoda, Acanthocephala, Hirudinea, Crustacea. The largest number of species belongs to trematodes, the rest, taxa (mixo- and microsporidia, cestodes, nematodes, acanthocephalans, leeches and parasitic crustaceans), are represented singly. We calculated the species diversity ratio (R) of fish parasites in the reservoirs, which ranged from 0.335 to 0.575, which indicates the fauna similarity from 50 to 75%. The greatest similarity in the parasite fauna was found in the roach (R = 0.168–0.447), the least in the European perch (R = 0.447–1.0). We have identified the dominant species that are found in more than 20% of the studied fish and determine the “core” of the parasitic fauna, which included 4 parasite species of epizootic and 1 parasite species of epidemiological significance.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42746075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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