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Preservation of goat semen at 5°C with emphasis on its freezability and the impact of melatonin 在5°C下保存山羊精液,重点研究其冷冻性和褪黑素的影响
Pub Date : 2019-07-10 DOI: 10.17352/IJVSR.000039
Ellakov Ka
Evaluation of goat semen, extended in Cornell University 16 extender, was used to study the effects of melatonin of various concentrations on sperm motility (SM) %, alive sperm (AS) % and sperm abnormalities (SA) % of extended goat semen stored at 5°C for seven days were studied. Melatonin at the doses of 0.0, 10.0, 15.0 and 20.0 μg/ 100 x 106 sperm was added to the extended semen and the samples were examined daily for the previous parameters. Additionally, the infl uence of melatonin on post-thawing motility was assessed.
采用康奈尔大学16号延长器延长山羊精液,研究了不同浓度褪黑素对延长5℃保存7 d的山羊精液的精子活力(SM) %、活精子(AS) %和精子异常(SA) %的影响。延长后的精液中分别添加剂量为0.0、10.0、15.0和20.0 μg/ 100 × 106个精子的褪黑激素,每天检测样品的上述参数。此外,褪黑素对解冻后运动的影响进行了评估。
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引用次数: 8
Antibacterial activity of honey and Nigella sativa L. seed extracts against animal wound bacteria 蜂蜜和黑草种子提取物对动物伤口细菌的抑菌活性
Pub Date : 2019-05-25 DOI: 10.17352/IJVSR.000038
Saïd Derbal, A. Niar
This study was designed to evaluate the antibacterial activity of Algerian honey and some extracts of Nigella sativa L. seeds against animal wound bacteria. To do this, the preparation of Nigella sativa L. seed extracts was carried out by macerating the seed powder in increasingly polar solvents (ethyl acetate, ethanol and methanol). The antibacterial activity of honey and Nigella sativa L. seed extracts against the bacterial isolates was studied by Agar Well Diffusion Assay. The results showed that the different honey concentrations (20%, 50%, 70% and 100% v/v) were found to be active against all the bacterial isolates. The different Nigella sativa L. seed extracts showed a variable activity at the concentration 300 mg/mL against the bacterial isolates. Ethanolic extract of Nigella sativa L. seeds was found to be active against all the bacterial isolates. Methanolic extract of Nigella sativa L. seeds was found to be active against all the bacterial isolates except Escherichia coli. Also, ethyl acetate extract of Nigella sativa L. seeds was found to be active against all the bacterial isolates except Enterococcus faecalis. In conclusion; Algerian honey (of the region of El-Eulma) and ethanolic extract of Nigella sativa L. seeds can be used as an alternative remedy for the treatment of different animal wounds.
本实验旨在研究阿尔及利亚蜂蜜和黑芝麻种子部分提取物对动物伤口细菌的抑菌作用。为此,将黑草种子粉末浸泡在极性越来越强的溶剂(乙酸乙酯、乙醇和甲醇)中,制备黑草种子提取物。采用琼脂孔扩散法研究了蜂蜜和黑草种子提取物对细菌分离株的抑菌活性。结果表明,不同蜂蜜浓度(20%、50%、70%和100% v/v)对所有菌株均有抑制作用。不同黑草种子提取物在300 mg/mL浓度下对细菌分离物的抑菌活性不同。结果表明,黑草种子乙醇提取物对所有菌株均有抑制作用。除大肠埃希氏菌外,黑草种子甲醇提取物对所有菌株均有抑制作用。此外,黑草种子乙酸乙酯提取物对除粪肠球菌外的所有细菌均有抑制作用。结论;阿尔及利亚蜂蜜(El-Eulma地区)和黑芝麻种子的乙醇提取物可作为治疗不同动物伤口的替代药物。
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引用次数: 4
In-vitro susceptibility of wound bacteria of domestic carnivores to different mixtures of honey and Nigella sativa L. seed extracts 家养食肉动物伤口细菌对不同蜂蜜和黑草种子提取物混合物的体外敏感性研究
Pub Date : 2019-05-14 DOI: 10.17352/IJVSR.000037
Derbal Saïd, Niar Abdellatif, Hanfer Mourad
The aim of this study is to evaluate the bactericidal activity of honey alone and honey mixed with different Nigella sativa L. seed extracts against bacterial isolates from chronic wounds of domestic carnivores. The preparation of Nigella sativa L. seed extracts was carried out by macerating the seed powder in increasingly polar solvents (ethyl acetate, ethanol and methanol). The study of the minimum bactericidal concentration of honey alone and honey mixed with different Nigella sativa L. seed extracts was carried out by Broth Dilution Method. The results showed that the addition of different Nigella sativa L. seed extracts decreased the bactericidal activity of honey against Escherichia coli and potentiated this bactericidal activity against Staphylococcus intermedius.
本研究旨在评价蜂蜜单独和蜂蜜与不同黑草种子提取物混合对家养食肉动物慢性伤口分离菌的杀菌活性。将黑草种子粉末浸泡在极性越来越强的溶剂(乙酸乙酯、乙醇和甲醇)中,制备黑草种子提取物。采用肉汤稀释法研究了蜂蜜单独和蜂蜜与不同黑草种子提取物混合的最低杀菌浓度。结果表明,添加不同黑草籽提取物可降低蜂蜜对大肠杆菌的杀菌活性,增强蜂蜜对中间葡萄球菌的杀菌活性。
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引用次数: 0
Demonstration and performance evaluation of dual purpose chicken “Potchefstroom Koekoek” under agro pastoral management condition at Asayta districts of Afar regional state in Ethiopia 埃塞俄比亚阿法尔州Asayta地区农牧管理条件下两用鸡“Potchefstroom Koekoek”的示范和性能评价
Pub Date : 2019-05-08 DOI: 10.17352/IJVSR.000036
A. Hassen
Dual purpose poultry package demonstration was undertaken at Awsi resu zone, Asayta district, Korodora kebele agro pastoral association with the objectives of demonstrating and evaluating the performance of chicken under agro pastoral management condition. Agro pastoral association and participant were selected purposively from the district, on the basis of experience and willingness to construct poultry house and cover all the associated package costs. A total of ten households were selected based on the package requirements.
在Korodora kebele农牧协会Asayta区Awsi resu区进行了双用途家禽包装示范,目的是示范和评价农牧管理条件下鸡的生产性能。根据经验和意愿,有目的地从地区中选择农牧协会和参与者来建造禽舍并承担所有相关的包装成本。根据包装要求,共选择了10个家庭。
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引用次数: 0
Bovine Hydatid Cyst: Prevalence, Characterization, Public Health and Economic Importance at adama Abattoir, Central Ethiopia 牛包虫囊肿:在埃塞俄比亚中部阿达玛屠宰场的流行、特征、公共卫生和经济重要性
Pub Date : 2019-03-14 DOI: 10.17352/IJVSR.000035
T. Belachew, Mekdes Abay, Teshome Gunse
A purposive study was conducted from November 2011 to April 2012 with the objective of determining the characteristics of hydatid cysts and to assess the current status of economic losses of hydatidosis in cattle slaughtered at Adama municipality abattoir. Hydatid cysts were characterized on the basis of their size, fertility and viability. Routine meat inspection procedure was conducted on organs namely lung, liver, kidney, heart and spleen involving visualization and palpation to detect the presence of hydatid cysts. Out of the total of 2276 hydatid cysts counted, 1126(49.5%), 913(40.1%) and 237(10.4%) were found to be small, medium and large size respectively. Out of the total of 300 cysts examined for the fertility test, 29(9.6%), 188(62.6%) and 83(27.6%) were found to be fertile, sterile and calcified respectively. The rate of cyst calcification was higher in the liver than in the lung while fertility rate was higher among the cysts of the lung. The direct financial loss during the study period was 8,749 ETB. This result was assessed from the mean retail market price of each organs multiplied by the total number of organs condemned at Adama abattoir during the study period.
2011年11月至2012年4月进行了一项目的研究,目的是确定阿达马市屠宰场屠宰牛的包虫病特征,并评估目前因包虫病造成的经济损失状况。包虫病的特征是基于它们的大小、生育能力和生存能力。对肺、肝、肾、心、脾等脏器进行常规肉品检查,包括目视和触诊检查包虫病的存在。在统计的2276个包囊中,小、中、大包囊分别为1126个(49.5%)、913个(40.1%)和237个(10.4%)。在接受生育测试的300个囊肿中,分别有29个(9.6%)、188个(62.6%)和83个(27.6%)被发现是可生育的、不育的和钙化的。肝囊肿钙化率高于肺囊肿,肺囊肿的生育率高于肺囊肿。研究期间的直接经济损失为8749 ETB。这一结果是用每个器官的平均零售市场价格乘以研究期间Adama屠宰场被判死刑的器官总数来评估的。
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引用次数: 1
Comparison of total Intravenous Ketamine and Propofol Anaesthesia in Acepromazine-Dexmedetomidine sedated cats 乙酰丙嗪-右美托咪定镇静猫全静脉氯胺酮与异丙酚麻醉的比较
Pub Date : 2019-01-17 DOI: 10.17352/IJVSR.000034
Oladapo Afolabi, Omowunmi C Oguntoye, O. Eyarefe, A. Adetunji
Total intravenous anaesthesia (TIVA) refers to the induction and maintenance of general anaesthesia with drugs administered solely by the intravenous (IV) route. Presently, ketamine and propofol are popular and in use for TIVA in small animals. This study compared ketamine and propofol anaesthesia in cats premedicated with acepromazine-dexmedetomidine combination. Six indigenous Nigerian local cats were premedicated with intramuscular injection of acepromazine (0.2mg/kg) and dexmedetomidine (40µg/kg) mixture, followed by induction of anesthesia with either bolus propofol (1mg/kg) or ketamine (0.5mg/kg) IV 20 min later. Maintenance of anesthesia for 2h was achieved with continous fluid administration using dose of 0.5ml/8ml and 0.05ml/8ml in lactated Ringers solution for propofol and ketamine respectively. One week space was allowed between protocols in the crossover experiment.
全静脉麻醉(TIVA)是指仅通过静脉(IV)途径给药的药物诱导和维持全身麻醉。目前,氯胺酮和异丙酚被广泛用于小动物的TIVA。本研究比较了氯胺酮和异丙酚在预先给药乙酰丙嗪-右美托咪定组合的猫的麻醉效果。对6只尼日利亚本地猫肌肉注射乙酰丙嗪(0.2mg/kg)和右美托咪定(40µg/kg)混合物,20 min后用异丙酚(1mg/kg)或氯胺酮(0.5mg/kg)静脉诱导麻醉。异丙酚和氯胺酮在乳酸林格液中分别以0.5ml/8ml和0.05ml/8ml的剂量持续给药,维持麻醉2h。在交叉实验中,两个方案之间留出一周的间隔。
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引用次数: 1
Does Dexmedetomidine Protect the Visceral Organs against the Asphxia 右美托咪定能保护内脏器官免受窒息吗
Pub Date : 2019-01-06 DOI: 10.17352/ijvsr.000033
A. Mızrak
Background: This study was conducted to determine whether dexmedetomidine exhibits a protective effect against sudden and short term hypoxia on brain, heart, lung, liver, and kidney in rat model which sudden hyperglycemia and hypertension developed.
背景:本研究旨在探讨右美托咪定对突发性高血糖和高血压大鼠模型的脑、心、肺、肝、肾是否具有抗突发性和短期缺氧的保护作用。
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引用次数: 0
Mesenchymal stem cells from the domestic ungulates: trends and outliers 来自家养有蹄类动物的间充质干细胞:趋势和异常值
Pub Date : 2018-12-21 DOI: 10.17352/IJVSR.000032
Anna A. Tvorogova, A. Kovaleva, A. Saidova
Mesenchymal stem cells (MSCs) are a valuable source for regenerative therapy and tissue engineering. MSCs are multipotent adherent stem cells that can be isolated from different adult and fetal tissues. In contrast to human MSCs, MSCs from large animal models have not yet been described by the uniform criteria, which include the characteristic phenotype of surface molecules, expression of stemness markers and differentiation potencies. The current review describes state of the art for characterization for MSCs from three species of domestic ungulates, including cattle (Bos taurus), swine (Sus scrofa) and sheep (Ovis aries). The comparative analysis of surface phenotype, gene expression and differentiation capacities of MSCs from different origins allows defining the consensus phenotype of bovine, ovine and porcine MSCs. We also discuss the major data discrepancies and pitfalls that are complicating the successful research of MScs from domestic livestock. This review emphasizes the pressing need for the unification of mesenchymal stem cell criteria in the veterinary field.
间充质干细胞(MSCs)是再生治疗和组织工程的重要来源。MSCs是一种多能贴壁干细胞,可以从不同的成人和胎儿组织中分离出来。与人类间充质干细胞相比,来自大型动物模型的间充质干细胞尚未被统一的标准描述,包括表面分子的特征表型、干性标记物的表达和分化能力。本综述描述了三种家养有蹄类动物(牛、猪和羊)间质干细胞表征的最新进展。通过对不同来源间充质干细胞表面表型、基因表达和分化能力的比较分析,可以确定牛、羊和猪间充质干细胞的一致表型。我们还讨论了使家畜间充质干细胞成功研究复杂化的主要数据差异和陷阱。本文强调了兽医间充质干细胞标准统一的迫切需要。
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引用次数: 2
Preparation and administration of medicaments body weight of animals and drug dosage 药物的制备和给药动物的体重和药物剂量
Pub Date : 2018-08-14 DOI: 10.17352/ijvsr.s1.104
T. Abdisa
The body condition of a livestock is generally assessed by visual observation. The references to an animal being too thin, in good fl esh, or obese all relate to the animal’s weight. Therefore, the weight of an animal can be used as a measurement tool to determine its well-being, or the presence of problems which may threaten the health of the horse. The weight of small animalsand poultry may be carried out by balance by automatic balance machine several methods can be used to determine the body weight of livestock in an approximate scale.
家畜的身体状况一般是通过目测来评估的。提到动物太瘦、精力充沛或肥胖,都与动物的体重有关。因此,动物的体重可以作为一种测量工具来确定它的健康状况,或者是否存在可能威胁马健康的问题。小动物和家禽的体重可以通过自动平衡机进行平衡,有几种方法可以在一个近似的尺度上确定牲畜的体重。
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引用次数: 0
Physical Examination 体格检查
Pub Date : 2018-08-14 DOI: 10.1201/b13528-7
Physical examination is the methods of examination by means of applying general inspection, palpation, percussion and auscultation of animals to detect clinical signs of patient animals. General inspection is done some distance away from the animal; sometimes go round the animal or herd/fl ock, in order to get the general impression about the case1
体格检查是通过对动物进行全身检查、触诊、叩诊和听诊,发现病人动物的临床体征的检查方法。一般检查是在离动物一定距离的地方进行的;有时要绕着动物或畜群/羊群走一圈,以获得对病例的总体印象
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引用次数: 0
期刊
International Journal of Veterinary Science and Research
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