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Exploring beliefs, intentions, and expectations towards cured meat alternatives: A qualitative analysis based on the theory of planned behaviour and product tasting 探索对腌肉替代品的信念、意图和期望:基于计划行为和产品品尝理论的定性分析
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.fufo.2026.100912
Stergios Melios , Niamh Harbourne , Declan Bolton , Emily Crofton
Cured meats consumption presents a dual challenge due to the presence of carcinogens formed from nitrites and the broader need to reduce overall meat intake. This study aimed to better understand individuals beliefs, intentions, and expectations of cured meat alternatives. This was done avoiding treating meat reduction as a general issue isolated from meat-based products, as is often the case, and instead focusing on a specific product category and cultural context, considering both plant-based and healthier, nitrite-free, meat-based alternatives as they appear in real-world decision-making contexts. To do so, in-depth, semi-structured interviews were conducted based on a combination of the Theory of Planned Behaviour with an experiential tasting component. Reflexive Thematic Analysis was then employed to enable a deeper exploration of psychological constructs alongside real-life responses. Results showed that while participants recognised environmental, health, and ethical benefits in plant-based cured meat alternatives, they also expressed concerns, particularly around social judgment, convenience, and perceived ambiguity about the products. Nitrite-free options were generally viewed more favourably, with clearer health benefits and fewer disadvantages. Cost emerged as a common barrier for both categories. Sensory expectations, perceptions of naturalness, and social influences shaped participants’ intentions. Interestingly, purchase intent increased after tasting for both categories. Regarding plant-based alternatives, ambiguity emerged as a key theme. Participants struggled to understand their diverse range and categorise them within familiar food groups, which reduced their perceived control over the products. These outcomes aim to inform future research employing quantitative analysis and to support interventions in the field of protein transition.
腌肉的消费面临着双重挑战,因为存在由亚硝酸盐形成的致癌物,而且更广泛地需要减少总体肉类摄入量。这项研究旨在更好地了解个人对腌肉替代品的信念、意图和期望。这样做是为了避免将减少肉类作为一个与肉类产品分离的一般问题,而不是像通常情况那样,将重点放在特定的产品类别和文化背景上,考虑到植物性和更健康的,无亚硝酸盐的,基于肉类的替代品,因为它们出现在现实世界的决策环境中。为了做到这一点,深入的、半结构化的访谈是基于计划行为理论和体验品尝成分的结合进行的。然后使用反身性主题分析来深入探索心理结构和现实生活反应。结果显示,虽然参与者认识到植物性腌肉替代品在环境、健康和道德方面的好处,但他们也表达了担忧,特别是在社会判断、便利性和对产品的模糊性方面。不含亚硝酸盐的选择通常更受欢迎,对健康的好处更明显,缺点更少。成本成为这两个类别的共同障碍。感官期望、自然感知和社会影响塑造了参与者的意图。有趣的是,在品尝了这两类产品后,购买意愿都有所增加。关于植物替代品,模糊性成为一个关键主题。参与者很难理解他们的多样化范围,并将他们归类为熟悉的食物组,这降低了他们对产品的控制。这些结果旨在为采用定量分析的未来研究提供信息,并支持蛋白质转化领域的干预措施。
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引用次数: 0
Tailoring bioactive properties of cricket powder through optimized hydrolysis using pineapple rhizome-derived bromelain 利用菠萝根茎衍生的菠萝蛋白酶优化水解蟋蟀粉的生物活性
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.fufo.2026.100914
Pilanee Vaithanomsat , Phornphimon Janchai , Nutthamon Boonlum , Chanaporn Trakunjae , Waraporn Apiwatanapiwat , Antika Boondaeng , Hataitip Nimitkeatkai , Amnat Jarerat
Crickets are rich in nutrients, but their direct consumption remains limited; thus, transforming them into hydrolyzed products may enhance their applicability. This study aimed to optimize the enzymatic hydrolysis of cricket powder using bromelain derived from pineapple rhizomes to obtain bioactive hydrolysates. Response surface methodology was applied to evaluate the effects of protein concentration, enzyme concentration, and hydrolysis period on the degree of hydrolysis (DH). The optimal conditions (6.51% protein, 1.5% enzyme and 155 min) produced a DH of 80.20%. The resulting hydrolysate contained 76.96% protein and exhibited peptide sizes ranging from 3.5 to 38 kDa. ATR-FTIR confirmed characteristic amide bands, indicating structural modifications following hydrolysis. The hydrolysate demonstrated enhanced antioxidant activities, including DPPH, superoxide, and nitric oxide radical scavenging, as well as higher ferric-reducing power compared with untreated cricket powder. It also showed strong ACE inhibitory activity, suggesting potential antihypertensive effects. Cytotoxicity assessment using L929 and Vero cell lines revealed no significant toxicity at concentrations up to 1,000 µg/mL. Overall, bromelain-mediated hydrolysis effectively improved the functional and bioactive properties of cricket powder, producing a safe peptide-rich ingredient with antioxidant and ACE inhibitory potential. These findings highlight the promise of cricket-based hydrolysates as sustainable and value-added components for future food applications.
蟋蟀含有丰富的营养,但它们的直接消耗仍然有限;因此,将其转化为水解产物可以提高其适用性。本研究旨在利用菠萝根茎中提取的菠萝蛋白酶对蟋蟀粉进行酶解,以获得具有生物活性的水解产物。采用响应面法评价蛋白浓度、酶浓度和水解时间对水解度(DH)的影响。最佳条件(6.51%蛋白质,1.5%酶,155 min) DH为80.20%。得到的水解产物蛋白含量为76.96%,肽大小为3.5 ~ 38 kDa。ATR-FTIR证实了特征酰胺带,表明水解后结构发生了变化。与未经处理的蟋蟀粉相比,水解产物显示出增强的抗氧化活性,包括清除DPPH、超氧化物和一氧化氮自由基,以及更高的铁还原能力。它还显示出较强的ACE抑制活性,提示潜在的抗高血压作用。使用L929和Vero细胞系进行的细胞毒性评估显示,浓度高达1,000 μ g/mL时无明显毒性。总的来说,菠萝蛋白酶介导的水解有效地提高了蟋蟀粉的功能和生物活性,产生了一种安全的富含肽的成分,具有抗氧化和抑制ACE的潜力。这些发现突出了蟋蟀水解物作为未来食品应用的可持续和增值成分的前景。
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引用次数: 0
Italian consumers perspectives on insect-based feeds for ruminants and derived foods products 意大利消费者对反刍动物昆虫饲料及其衍生食品的看法
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.fufo.2026.100915
Manuela Renna , Lara Rastello , Laura Gasco , Lisa Livorsi , Pablo G. Toral , Rachele De Cianni , Valentina Maria Merlino
A survey of 1040 Italian consumers was conducted to investigate their knowledge, acceptance, and willingness to consume food products obtained from ruminants fed diets containing insect-derived products. The questionnaire covered the following sections: 1) consumption of ruminant-derived foods; 2–3) opinion on using insect-based feed for farm animals in general and specifically for ruminants; 4) the sustainability of insect-based feed for ruminants; 5) related benefits and risks; 6) perceived positive externalities from insect meals and oils for ruminant feeding; 7) knowledge of legislation on the use of insects as feed; and 8) concerns about insect-based feed for ruminants. Socio-demographic (SD) data was also collected. Sections 2, 3 and 4 were used in a Principal Component Analysis (PCA) to obtain perception and attitudinal patterns towards insect-based feed use in farm animal nutrition. The PCA defined three main components which were subsequently used as input for a Cluster Analysis, which categorized consumers into four groups. "Moderate rejectors" viewed insects as animal feed with some reservations. "Sustainability-driven" consumers supported insect-based feed for farm animals. "Insect-based feed supporters" were in favour but raised sustainability concerns. "Purpose-driven" consumers accepted insect-based feed, but not as a long-term solution. Clusters were characterised in terms of SD characteristics, knowledge of legislation, benefits, risks, externalities, and concerns related to insect-based feed for ruminants, also comparing different ruminant-derived food products. This study identifies specific consumer groups and provides insights into communication, policy and decision-making strategies for a sustainable livestock sector.
对1040名意大利消费者进行了一项调查,以了解他们对以含有昆虫衍生产品的反刍动物为食的食品的了解、接受程度和消费意愿。问卷内容包括以下几个部分:1)反刍动物源性食品的消费情况;2-3)对一般农场动物,特别是反刍动物使用昆虫饲料的意见;4)反刍动物昆虫饲料的可持续性;5)相关利益和风险;6)反刍动物食用昆虫饲料和昆虫油的正外部性;7)了解以昆虫为饲料的法例;8)对反刍动物的昆虫饲料的担忧。还收集了社会人口统计学(SD)数据。第2、3和4节用于主成分分析(PCA),以获得对农场动物营养中昆虫饲料使用的感知和态度模式。PCA定义了三个主要成分,这些成分随后被用作聚类分析的输入,聚类分析将消费者分为四组。“适度拒绝者”有保留地将昆虫视为动物饲料。“可持续发展”的消费者支持以昆虫为原料的饲料。“昆虫饲料支持者”对此表示支持,但提出了可持续性问题。“目的导向”的消费者接受了昆虫饲料,但不是长期解决方案。根据SD特征、立法知识、利益、风险、外部性和与反刍动物昆虫饲料相关的问题对集群进行了表征,并比较了不同的反刍动物衍生食品。这项研究确定了特定的消费群体,并为可持续畜牧业的沟通、政策和决策战略提供了见解。
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引用次数: 0
Impact of pickling and brining on the perceptual and nutritional characteristics of sea lettuce (Ulva fenestrata) 酸洗和盐水处理对海莴苣感官和营养特性的影响
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.fufo.2026.100913
Sara Björkman , Jessica Jenemyr , Karin Wendin , Madeleine Jönsson
Seaweed is a promising candidate as a healthy, tasty, and sustainable future food in Western cultures, but currently lacks gastronomic applications in these settings. Therefore, this study aimed to refine the green seaweed Ulva fenestrata into sustainable, nutritious, and appealing food products and evaluate its quality and consumer acceptance. Five products – rehydrated Ulva (control), brined (6% NaCl), pickled in acetic acid, and pickled in cold and warm apple cider vinegar – were developed and assessed for nutritional, safety, and sensory characteristics. The raw material contained all essential amino acids at levels qualifying it as a complete protein and was rich in essential minerals such as calcium, potassium, magnesium, sodium, and sulfur. Acid treatments using acetic acid and apple cider vinegar effectively reduced undesirable elements, including arsenic, cadmium, and iodine. While pickling decreased hardness and greenness, brining preserved these physical attributes. Aroma profiles determined by SPME-GC–MS (solid-phase microextraction followed by gas chromatography with mass spectrometry) varied significantly across treatments: control and brined samples were dominated by dimethyl sulfide and 2-aziridinylethyl-amine, whereas pickled samples exhibited complex profiles characterized by aldehydes and acetic acid, imparting fermented, pungent, and fruity notes. Sensory evaluation by quantitative descriptive analysis (QDA) with trained experts revealed that brining enhanced seaweed odors, umami, and saltiness, while vinegar pickling introduced sweetness and distinctive vinegar-related flavors. Most importantly, the intense bitterness and fishy flavors from dimethyl sulfide were reduced in all treatments, especially in pickled products. Consumer acceptance was high for pickled products, indicating strong potential for culinary applications.
在西方文化中,海藻是一种健康、美味和可持续的未来食物,但目前在这些环境中缺乏烹饪应用。因此,本研究旨在将绿海藻精制成可持续的、营养丰富的、有吸引力的食品,并评估其质量和消费者接受度。开发了五种产品——复水合Ulva(对照)、盐水(6% NaCl)、醋酸腌制、冷苹果醋和温苹果醋腌制——并对其营养、安全性和感官特性进行了评估。这种原料含有所有必需的氨基酸,其含量使其成为一种完整的蛋白质,并富含必需的矿物质,如钙、钾、镁、钠和硫。酸处理使用醋酸和苹果醋有效地减少不需要的元素,包括砷,镉和碘。酸洗降低了硬度和绿度,而盐水保留了这些物理属性。SPME-GC-MS(固相微萃取-气相色谱-质谱)测定的香气谱在不同处理下差异显著:对照和盐水样品主要由二甲硫醚和2-亚氮基乙胺组成,而腌制样品则以醛和乙酸为特征,呈现出发酵、辛辣和水果味。由训练有素的专家通过定量描述性分析(QDA)进行的感官评估显示,盐水增强了海藻的气味、鲜味和咸味,而醋酸洗则引入了甜味和独特的醋相关风味。最重要的是,二甲基硫化物的强烈苦味和鱼腥味在所有处理中都有所减少,特别是在腌制产品中。消费者对腌制产品的接受度很高,表明烹饪应用的潜力很大。
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引用次数: 0
“It takes the identity out of the food”: Soldiers’ perceptions of 3D-printed food “它让食物失去了身份”:士兵对3d打印食物的看法
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-13 DOI: 10.1016/j.fufo.2026.100906
Jonathan Blutinger, Alan Wright, Michael Okamoto
Ensuring acceptance of novel food technologies is nearly as vital as advancing the technology itself. Three-dimensional (3D) printing is an example of advanced manufacturing being applied towards tailored food production. 3D food printing (3DFP) provides benefits of personalized nutrition, deployable food production, customized aesthetic expression, and optimized performance. These attributes may not be readily apparent—especially those positioned to use 3DFP in the future. Military applications and government-funded efforts currently supporting this technology, make it important to understand perceptions of large potential user populations like U.S. Army Soldiers. At the DEVCOM Soldier Center, we conducted a set of focus groups and sensory panels with 17 voluntary, military respondents. Two group panels were conducted to better understand preconceived notions and attitudes of Soldiers about 3DFP technology, 3DFP food attributes, 3DFP food labels and naming conventions, and 3DFP products they would want to see developed. Initially, Soldiers showed skepticism and reluctance towards use of the technology. However, after 3DFP technology was explained and 3D-printed prototypes were provided, Soldiers’ acceptance increased considerably. Novel 3DFP prototypes with some visual familiarity tended to score higher than prototypes that were unfamiliar. Individual differences of affect between panelists were most apparent for flavor and texture attributes. Keywords related to appearance and texture were discussed most with regards to 3DFP.
确保人们接受新的食品技术几乎与推进技术本身同样重要。三维(3D)打印是先进制造应用于定制食品生产的一个例子。3D食品打印(3DFP)提供了个性化营养、可部署食品生产、定制美学表达和优化性能的好处。这些属性可能不太明显——尤其是那些在未来使用3DFP的人。军事应用和政府资助的目前支持这项技术的努力,使得了解像美国陆军士兵这样的大量潜在用户群体的看法变得重要。在DEVCOM士兵中心,我们对17名自愿的军人进行了一系列焦点小组和感官面板调查。为了更好地了解士兵对3DFP技术、3DFP食品属性、3DFP食品标签和命名惯例以及他们希望开发的3DFP产品的先入为主的观念和态度,我们进行了两次小组讨论。最初,士兵们对使用这项技术表示怀疑和不情愿。然而,在解释了3DFP技术并提供了3d打印原型后,士兵的接受度大大提高。具有一定视觉熟悉度的新颖3DFP原型往往比不熟悉的原型得分更高。小组成员之间的个体差异对风味和质地属性的影响最为明显。3DFP中讨论最多的关键词是外观和纹理。
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引用次数: 0
Denaturation dynamics of soy protein isolate for electrospinning: A comparative study of thermal, cold plasma, and high-pressure treatments 静电纺丝用大豆分离蛋白的变性动力学:热、冷等离子体和高压处理的比较研究
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-13 DOI: 10.1016/j.fufo.2026.100911
Danial Dehnad , Behrouz Ghorani , Bahareh Emadzadeh , Seid Mahdi Jafari
To date, no comparative analysis has been conducted regarding the effectiveness of cold plasma (CP) techniques—comprising both vacuum CP (VCP) and atmospheric CP (ACP)—high hydrostatic pressure (HHP), and thermal treatment (ThT) on protein denaturation prior to the electrospinning (ESP). This comparative evaluation is essential, as the current solution (ThT) to improving protein quality before ESP results in significant organoleptic consequences (e.g., color degradation and off-flavor) and nutrient loss. The expected impact of the study is to advance current knowledge of the denaturation dynamics of soy protein isolate (SPI) using green technologies, including VCP, ACP, and HHP. The main aim of the study was to investigate the suitability of green non-thermal technologies to replace inefficient conventional ThT. Thus, different properties of treated SPI samples, including solubility, surface hydrophobicity, sulfhydryl content, turbidity, secondary structure, viscosity and electrophoretic pattern were studied. While physicochemical properties, including solubility, secondary structure, and viscosity of SPI, were improved by CP techniques and HHP, ThT had an edge over non-ThTs in terms of denaturation before ESP. For example, ThT resulted in the highest SPI solubility (39%), sulfhydryl content (19%), turbidity value (5.19), and disordered structure (62.2%). Among non-ThTs, VCP dominated ACP and HHP in different characteristics, e.g., sulfhydryl content, viscosity, disordered structure, random coil content, and α-helix to β-sheet ratio. VCP preserved α-helix better due to oxidation-induced stability and reduced aggregation, while HHP increased β-sheet formation due to pressure-induced unfolding and aggregation. VCP generates reactive oxygen and nitrogen species, inducing mild amino acid oxidation that stabilizes α-helix via intra-molecular hydrogen bonding while preventing extensive unfolding.
迄今为止,还没有对冷等离子体(CP)技术(包括真空CP (VCP)和常压CP (ACP))、高静水压力(HHP)和热处理(ThT)在静电纺丝(ESP)之前对蛋白质变性的有效性进行比较分析。这种比较评估是必不可少的,因为目前在ESP之前提高蛋白质质量的解决方案(ThT)会导致显著的感官后果(例如,颜色退化和异味)和营养损失。该研究的预期影响是利用绿色技术(包括VCP、ACP和HHP)推进大豆分离蛋白(SPI)变性动力学的现有知识。该研究的主要目的是调查绿色非热技术取代低效传统高温的适用性。因此,研究了处理过的SPI样品的不同性质,包括溶解度、表面疏水性、巯基含量、浊度、二级结构、粘度和电泳模式。虽然CP技术和HHP改善了SPI的物理化学性质,包括溶解度、二级结构和粘度,但在ESP前变性方面,ThT比非ThT有优势。例如,ThT导致SPI溶解度最高(39%),巯基含量(19%),浊度值(5.19)和无序结构(62.2%)。在非thts中,VCP在巯基含量、粘度、无序结构、随机线圈含量、α-螺旋与β-片比等不同特征上均优于ACP和HHP。VCP由于氧化诱导的稳定性和聚集减少而更好地保存了α-螺旋,而HHP由于压力诱导的展开和聚集而增加了β-薄片的形成。VCP产生活性氧和活性氮,诱导轻微的氨基酸氧化,通过分子内氢键稳定α-螺旋,同时防止广泛展开。
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引用次数: 0
Protein informatics: Development and validation of a universal NIR spectroscopy-based deep learning and chemometric models for protein quantification in legume crops—A high-throughput approach for large germplasm screening 蛋白质信息学:豆科作物中基于通用近红外光谱的深度学习和化学计量模型的开发和验证-一种用于大型种质筛选的高通量方法
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-12 DOI: 10.1016/j.fufo.2026.100909
Simardeep Kaur , Siddhant Ranjan Padhi , Mithra T․ , Naseeb Singh , Maharishi Tomar , Racheal John , Amit Kumar , Veerendra Kumar Verma , Mohar Singh , Kuldeep Tripathy , Gayacharan , Vinod Kumar , Rajwant K. Kalia , Amit Kumar Singh , Dhammaprakash Pandhri Wankhede , Jai Chand Rana , Rakesh Bhardwaj , Amritbir Riar
Rapid, eco-friendly, and non-destructive estimation of protein content is crucial for efficient nutritional phenotyping and large-scale germplasm screening in legumes. Traditional biochemical methods are time-consuming, costly, and labor-intensive, posing challenges to breeders and the food industry. This study aimed to develop and validate universal near-infrared spectroscopy (NIRS)-based predictive models for protein quantification across multiple legume species. A genetically diverse dataset comprising 1,169 grain samples from cowpea, mung bean, horse gram, pea, lentil, faba bean, winged bean, adzuki bean, rice bean, lablab bean, and chickpea was utilized. Spectral data (1100–2498 nm) were preprocessed using Standard Normal Variate, detrending, derivatives, and smoothing techniques. Two models; Modified Partial Least Squares (MPLS) and one-dimensional Convolutional Neural Network (1D CNN) were developed and validated on an independent set of 351 samples. The 1D CNN model outperformed MPLS, achieving R² = 0.883 and RPD = 2.932, compared to MPLS (R² = 0.814; RPD = 2.320), demonstrating greater accuracy and robustness. This is the first report of a universal NIRS-based deep learning model for protein prediction across diverse legumes. Its integration into portable NIR sensors can accelerate field-based protein screening, enhancing breeding efficiency, gene bank evaluations, food quality control, and the development of functional foods.
快速、环保、无损的蛋白质含量估算对豆类植物营养表型分析和大规模种质筛选至关重要。传统的生化方法耗时长、成本高、劳动密集,给育种者和食品工业带来了挑战。本研究旨在建立并验证基于通用近红外光谱(NIRS)的多品种豆科植物蛋白质定量预测模型。利用了一个遗传多样性数据集,包括来自豇豆、绿豆、马革、豌豆、扁豆、蚕豆、羽绒豆、小豆、米豆、lablab bean和鹰嘴豆的1169个谷物样本。光谱数据(1100-2498 nm)使用标准正态变量、去趋势、导数和平滑技术进行预处理。两个模型;改进的偏最小二乘法(MPLS)和一维卷积神经网络(1D CNN)在351个独立样本上进行了开发和验证。与MPLS (R²= 0.814,RPD = 2.320)相比,1D CNN模型优于MPLS, R²= 0.883,RPD = 2.932,显示出更高的准确性和鲁棒性。这是第一份基于nir的通用深度学习模型的报告,用于预测不同豆类的蛋白质。将其集成到便携式近红外传感器中,可以加速现场蛋白质筛选,提高育种效率,基因库评估,食品质量控制和功能食品的开发。
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引用次数: 0
Effect of pistachio hull extract incorporation on the physical, mechanical, thermal, antifungal, and antioxidant properties of low-density polyethylene film for active packaging 掺入开心果壳提取物对活性包装用低密度聚乙烯薄膜物理、机械、热、抗真菌和抗氧化性能的影响
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-12 DOI: 10.1016/j.fufo.2026.100910
Mozhdeh Golestani , Abolfazl Pahlevanlo , Mostafa Shahidi Noghabi , Mahboobe Sarabi-Jamab , Hamid Salehi-Mobarakeh
Utilizing agro-industrial waste in active packaging can simultaneously address environmental concerns and promote public health by delivering bioactive compounds. In this study, low-density polyethylene (LDPE) films incorporating pistachio (Pistacia vera L.) hull extract (PHE) were fabricated via extrusion at concentrations of 0.5, 1, 2, 4, and 8 wt.%. Among the formulations, the film containing 2 wt.% PHE demonstrated a sustained release of active compounds, resulting in significant antifungal and antioxidant activities. Therefore, detailed structural, thermal, and mechanical characterizations focused on this formulation. SEM revealed a heterogeneous distribution of PHE aggregates within the film. FTIR and XRD analyses confirmed the presence of PHE and an increase in crystallinity. DSC and TGA demonstrated improved thermal stability. However, incorporating PHE significantly reduced tensile strength and elongation at break (P < 0.05), while increasing water vapor transmission rate and oxygen permeability. These results suggest that PHE can function as an effective natural additive to develop bioactive LDPE-based packaging materials.
在活性包装中利用农业工业废物可以同时解决环境问题,并通过提供生物活性化合物促进公众健康。在这项研究中,通过挤压制备了含有开心果(Pistacia vera L.)外壳提取物(PHE)的低密度聚乙烯(LDPE)薄膜,其浓度分别为0.5、1、2、4和8wt .%。在配方中,含有2 wt.% PHE的薄膜显示出活性化合物的持续释放,产生显着的抗真菌和抗氧化活性。因此,详细的结构,热学和力学特性集中在这个配方。扫描电镜显示膜内PHE聚集体分布不均。FTIR和XRD分析证实了PHE的存在和结晶度的增加。DSC和TGA显示了更好的热稳定性。然而,添加PHE显著降低了抗拉强度和断裂伸长率(P < 0.05),同时增加了水蒸气透过率和氧气渗透率。这些结果表明,PHE可以作为一种有效的天然添加剂来开发具有生物活性的ldpe基包装材料。
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引用次数: 0
Cell culture media for cultivated meat: Review and perspectives on first principles design to drive cost-effective scale-up 用于培养肉类的细胞培养基:对第一原则设计的回顾和观点,以推动成本效益的扩大
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-12 DOI: 10.1016/j.fufo.2026.100908
Corbin M. Goodwin , Mahe Jabeen , Balaji M. Rao , Rohan A. Shirwaiker

Background

Cultivated meat (CM) has the potential to complement conventional meat while reducing the environmental footprint of food production to meet the growing global protein demand. Cell culture medium is not only a critical production input but also a major cost-driver, estimated to contribute to 31–99 % of CM production costs at scale. Typical culture media optimized for biomedical applications are unsuitable for CM due to their high costs and their reliance on animal-derived components such as serum, which is poorly chemically-defined.

Scope and approach

Here, we review recent advancements in culture media development for bovine, chicken, and porcine CM applications and propose a first principles approach for cost-effective CM media development.

Key findings and conclusions

The majority of efforts to reduce media costs by eliminating serum, optimizing growth factors, and replacing animal-derived components with alternatives including from recombinant sources have fallen short of achieving stable long term cultures with optimal cell proliferation and differentiation rates to achieve high quality CM biomass. Our current understanding of the roles of key media components such as glucose, lipids, growth factors, and hormones is limited, which is prolonging much needed cost-effective innovations in CM media. We propose investigating and prioritizing essential components for CM-specific cell physiology and metabolism while eliminating unnecessary additives, then employing technoeconomic analysis for further optimization towards scale-up production. This targeted approach can help create cost-effective serum- or animal-free culture medium solutions to enable scalable CM production and help accelerate adoption of CM as a sustainable protein source.
栽培肉(CM)具有补充传统肉类的潜力,同时减少粮食生产的环境足迹,以满足日益增长的全球蛋白质需求。细胞培养基不仅是关键的生产投入,也是主要的成本驱动因素,估计占大规模CM生产成本的31 - 99%。为生物医学应用而优化的典型培养基不适合CM,因为它们的成本高,而且依赖于动物来源的成分,如血清,而血清的化学定义很差。在这里,我们回顾了牛、鸡和猪CM应用的培养基开发的最新进展,并提出了具有成本效益的CM培养基开发的第一原则方法。通过去除血清、优化生长因子和用重组来源的替代品替代动物来源的成分来降低培养基成本的大多数努力都未能实现稳定的长期培养和最佳的细胞增殖和分化率,以获得高质量的CM生物量。我们目前对关键培养基成分(如葡萄糖、脂质、生长因子和激素)的作用的理解是有限的,这延长了CM培养基中急需的成本效益创新。我们建议研究和优先考虑cm特异性细胞生理和代谢的基本成分,同时消除不必要的添加剂,然后采用技术经济分析进一步优化,以实现规模化生产。这种有针对性的方法可以帮助创建具有成本效益的无血清或无动物培养基解决方案,以实现可扩展的CM生产,并有助于加速CM作为可持续蛋白质来源的采用。
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引用次数: 0
Harnessing cold plasma for sustainable extraction of bioactive compounds: a critical review of mechanisms and applications 利用冷等离子体可持续提取生物活性化合物:机制和应用综述
IF 8.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-10 DOI: 10.1016/j.fufo.2026.100907
Seyed Alireza Banihashemi , Saber Amiri , Elham Assadpour , Mohammad Yousefi , Seid Mahdi Jafari
Cold plasma (CP) represents a transformative, non-thermal approach for enhancing the extraction efficiency of bioactive compounds from diverse plant matrices. This review provides a critical synthesis of recent advances in CP-assisted extraction, emphasizing its mechanisms, optimization parameters, and comparative effectiveness relative to conventional and emerging extraction methods. CP operates through the synergistic action of reactive oxygen and nitrogen species, inducing cell wall disruption, surface activation, and in some cases, induced metabolic responses within plant tissues. Comprehensive analysis reveals that treatment parameters, including voltage, gas composition, and exposure time, have significant, compound-specific impacts on extraction yield and bioactive stability. Notably, CP enhances the recovery of polyphenols, flavonoids, and essential oils from multiple plant types, often outperforming conventional solvent-based techniques while aligning with green chemistry principles. However, improper parameter optimization can lead to oxidative degradation, particularly in thermolabile compounds like carotenoids and volatile oils. The review identifies key research gaps in the standardization of CP protocols, matrix-specific optimization, and industrial scalability. Future research should focus on mechanistic elucidation, post-extraction stability, and integration with complementary technologies to fully harness the potential of CP in sustainable food and pharmaceutical applications.
冷等离子体(CP)代表了一种变革性的、非热的方法,用于提高从各种植物基质中提取生物活性化合物的效率。本文综述了cp辅助提取的最新进展,强调了其机制、优化参数以及相对于传统和新兴提取方法的比较效果。CP通过活性氧和活性氮的协同作用起作用,诱导细胞壁破坏、表面活化,并在某些情况下诱导植物组织内的代谢反应。综合分析表明,处理参数,包括电压、气体组成和暴露时间,对提取率和生物活性稳定性有显著的、特定的影响。值得注意的是,CP提高了多酚、类黄酮和多种植物精油的回收率,通常优于传统的溶剂基技术,同时符合绿色化学原则。然而,不适当的参数优化可能导致氧化降解,特别是在类胡萝卜素和挥发油等耐热性化合物中。该综述指出了CP协议标准化、矩阵特定优化和工业可扩展性方面的关键研究差距。未来的研究应集中在机理阐明、提取后稳定性以及与互补技术的整合上,以充分利用CP在可持续食品和制药应用中的潜力。
{"title":"Harnessing cold plasma for sustainable extraction of bioactive compounds: a critical review of mechanisms and applications","authors":"Seyed Alireza Banihashemi ,&nbsp;Saber Amiri ,&nbsp;Elham Assadpour ,&nbsp;Mohammad Yousefi ,&nbsp;Seid Mahdi Jafari","doi":"10.1016/j.fufo.2026.100907","DOIUrl":"10.1016/j.fufo.2026.100907","url":null,"abstract":"<div><div>Cold plasma (CP) represents a transformative, non-thermal approach for enhancing the extraction efficiency of bioactive compounds from diverse plant matrices. This review provides a critical synthesis of recent advances in CP-assisted extraction, emphasizing its mechanisms, optimization parameters, and comparative effectiveness relative to conventional and emerging extraction methods. CP operates through the synergistic action of reactive oxygen and nitrogen species, inducing cell wall disruption, surface activation, and in some cases, induced metabolic responses within plant tissues. Comprehensive analysis reveals that treatment parameters, including voltage, gas composition, and exposure time, have significant, compound-specific impacts on extraction yield and bioactive stability. Notably, CP enhances the recovery of polyphenols, flavonoids, and essential oils from multiple plant types, often outperforming conventional solvent-based techniques while aligning with green chemistry principles. However, improper parameter optimization can lead to oxidative degradation, particularly in thermolabile compounds like carotenoids and volatile oils. The review identifies key research gaps in the standardization of CP protocols, matrix-specific optimization, and industrial scalability. Future research should focus on mechanistic elucidation, post-extraction stability, and integration with complementary technologies to fully harness the potential of CP in sustainable food and pharmaceutical applications.</div></div>","PeriodicalId":34474,"journal":{"name":"Future Foods","volume":"13 ","pages":"Article 100907"},"PeriodicalIF":8.2,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Future Foods
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