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Enhancing 3D printing performance of O/W emulsions with asparagus fibre 用芦笋纤维提高水包油型乳液的 3D 打印性能
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-12 DOI: 10.1016/j.fufo.2024.100472
Yifeng Lu, Maarten A.I. Schutyser, Lu Zhang
Asparagus fibre recovered from agricultural waste stream can be valorised as a natural ingredient for fibre enrichment in food products. This study investigated the effects of asparagus fibre on the 3D printing performance of oil-in-water (O/W) emulsions as edible inks. Emulsion gels were prepared by either varying the fibre concentration in the aqueous phase or varying the volume fraction of the oil phase. Printability, rheological properties, and microstructure of the samples were systematically evaluated. Our results show increasing fibre concentration (up to 9.9 %w/w) significantly improved 3D printing performance of the inks, which was attributed to their enhanced rheological properties. Interestingly, reducing the oil volume fraction from 72 to 66 %v/v (i.e., fibre concentration was kept at 9.9 %w/w) did not detrimentally influence the 3D printing quality. Microstructural analysis of the emulsions revealed that increasing fibre concentration led to smaller size and different surface morphology of the oil droplets. Fibre particles in the aqueous phase may hinder oil droplet movement, stabilizing emulsions during 3D printing. Our findings give new insights into the development of edible 3D-printed food products via fibre enrichment and oil reduction.
从农业废料流中回收的芦笋纤维可作为一种天然成分用于丰富食品中的纤维。本研究调查了芦笋纤维对作为食用油墨的水包油(O/W)乳液的三维打印性能的影响。通过改变水相中的纤维浓度或油相的体积分数来制备乳液凝胶。对样品的可印刷性、流变特性和微观结构进行了系统评估。我们的结果表明,增加纤维浓度(高达 9.9 %w/w)可显著提高油墨的三维打印性能,这归功于其流变特性的增强。有趣的是,将油的体积分数从 72%v/v 降低到 66%v/v(即纤维浓度保持在 9.9%w/w)并没有对 3D 打印质量产生不利影响。乳液的微观结构分析表明,纤维浓度的增加会导致油滴的尺寸变小,表面形态发生变化。水相中的纤维颗粒可能会阻碍油滴的移动,从而在 3D 打印过程中稳定乳剂。我们的研究结果为通过富集纤维和减少油分来开发可食用的 3D 打印食品提供了新的思路。
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引用次数: 0
Optimization of the decanter process in terms of the protein yield for the manufacture of an oat-based milk substitute 从生产燕麦代乳品的蛋白质产量角度优化滗水器工艺
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-10 DOI: 10.1016/j.fufo.2024.100470
Jonas Körber, Adrian Körzendörfer
Even though oat-based milk substitutes offer numerous nutritional benefits, one of their major disadvantages is the low protein content. To overcome this issue, the impact of the decanter process on the protein yield was investigated. A design of experiments with 30 experimental runs was performed to determine the influence of acceleration, feed, feed temperature, inner weir diameter, and differential speed on the separation process. The results were used to calculate the ideal parameters. Accordingly, an acceleration of 4,000 g, a feed of 20 kg/h, a temperature of 20 °C, an inner weir diameter of 68 mm, and a differential speed of 180 min−1 resulted in the highest theoretical protein in dry matter content (14.22 % w/w) corresponding to a protein yield of 98.2 %. The validation experiments with the calculated parameters led to a centrate with a protein in dry matter content of 11.63 ± 0.14 % (w/w), an absolute protein content of 1.00 ± 0.01 % (w/w), and a protein yield of 81.3 ± 1.70 %. The centrate was free of dark particles confirming an adequate separation of undesired solids. SDS-PAGE further proved that no protein fraction was completely separated. In conclusion, the parameters of the decanter process should be set carefully in order to achieve a high protein yield.
尽管燕麦代乳品具有多种营养益处,但其主要缺点之一是蛋白质含量较低。为了解决这个问题,我们研究了滗水器工艺对蛋白质产量的影响。通过 30 次实验设计,确定了加速度、进料、进料温度、内堰直径和差速对分离过程的影响。实验结果用于计算理想参数。因此,4,000 克的加速度、20 千克/小时的进料量、20 °C的温度、68 毫米的内堰直径和 180 分钟/1 的差速可产生最高的干物质理论蛋白质含量(14.22 % w/w),相当于 98.2 % 的蛋白质产量。根据计算参数进行的验证实验得出,干物质蛋白质含量为 11.63 ± 0.14 %(重量比),蛋白质绝对含量为 1.00 ± 0.01 %(重量比),蛋白质产量为 81.3 ± 1.70 %。中心液中没有深色颗粒,证明已充分分离了不需要的固体。SDS-PAGE 进一步证明,没有一个蛋白质部分被完全分离。总之,为了获得较高的蛋白质产量,滗水器工艺的参数应仔细设定。
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引用次数: 0
Partially defatted chia flour as a novel ingredient for gluten-free breadmaking: Improving sustainability, nutrition and consumer liking 部分脱脂奇异果粉作为无麸质面包的新型配料:改善可持续性、营养和消费者喜好
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-10 DOI: 10.1016/j.fufo.2024.100475
Fernanda Garcia dos Santos , Luciana Sandri , Urszula Krupa-Kozak , Vanessa Dias Capriles
Partially defatted chia flour (DCF) is a valuable food ingredient in the context of a circular economy, with the potential to improve the nutrient and bioactive contents of gluten-free foodstuff. Thus, this study assessed the effects of interactions between DCF and water content (W) on the physical and sensory properties of gluten-free bread (GFB) and defined, using the response surface method, the composition of formulations that enable quality optimization. A rotatable central composite design was used to assess the main and interaction effects of DCF (ranging from 0 to 28% on a flour basis (fb) and W (ranging from 100 to 200% fb) on the physical properties and consumer linking of rice flour based GFB. The resulting models indicated that higher DCF or W levels diminished the bread quality. However, different W and up to 17.2% DCF can be used to prepare bread with high consumer liking (scores ≥7 for all assessed characteristics, on a scale of 10). The formulation prepared with 0% DCF and 135.5% W (fb) had the highest degree of liking (varying from 8.3 to 9.0). However, when incorporating up to 9.8% DCF and 135.5% W, a high liking can be maintained (scores 8.0 to 8.5 for all characteristics). Acceptable GFB with up to 17.2% DCF in the formulation can be obtained if the W content is adjusted (135 to 139.7% fb). Incorporating 17.2% DCF doubles the fiber content of the GFB. Therefore, DCF is an alternative ingredient to obtain nutritionally improved and acceptable GFB.
部分脱脂奇异果粉(DCF)是循环经济背景下一种有价值的食品配料,具有提高无麸质食品的营养和生物活性含量的潜力。因此,本研究评估了 DCF 和水含量(W)之间的相互作用对无麸质面包(GFB)物理和感官特性的影响,并采用响应面法确定了可实现质量优化的配方组成。采用可旋转的中心复合设计来评估 DCF(以面粉为基准,从 0% 到 28% 不等)和 W(以面粉为基准,从 100% 到 200% 不等)对以米粉为基础的无麸质面包的物理特性和消费者联系的主要效应和交互效应。结果表明,较高的 DCF 或 W 水平会降低面包质量。然而,使用不同的 W 和高达 17.2% 的 DCF 可以制备出消费者非常喜欢的面包(在 10 分制中,所有评估特征的得分均≥7 分)。用 0% DCF 和 135.5% W 配制的配方(fb)消费者的喜爱程度最高(从 8.3 到 9.0 不等)。然而,当添加高达 9.8% 的 DCF 和 135.5% W 时,仍能保持较高的喜好度(所有特征的评分都在 8.0 到 8.5 之间)。如果调整 W 含量(135% 至 139.7%fb),配方中 DCF 含量高达 17.2%时,可获得可接受的 GFB。加入 17.2% 的 DCF 可使 GFB 的纤维含量增加一倍。因此,DCF 是一种替代配料,可用于获得营养上得到改善且可接受的 GFB。
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引用次数: 0
Designing sustainable antioxidant and anti-inflammatory ingredients from seaweeds for functional gummies 为功能性软糖设计可持续的海藻抗氧化和消炎成分
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-09 DOI: 10.1016/j.fufo.2024.100474
Bruno Toribio de Lima Xavier , Marcelo Franchin , Nima Mohammadi , Carolina Girotto Pressete , Lusânia Maria Greggi Antunes , Yandong Xu , Kai Wang , Jason Bennett , Daniel Granato
Atlantic wakame (AW) and sea lettuce (SL) are edible seaweeds commonly produced in Asia and Europe, typically consumed as fresh or dried biomass. Because of the short shelf life, developing new post-harvest treatments, such as the extraction of bioactive compounds, is essential for establishing reliable macroalgae supply chains for food applications. This study aims to develop a sustainable and functional seaweed-based food ingredient rich in bioactive compounds for functional food applications. The results indicated that ultrasound-assisted extraction using 70% ethanol for 10 min was optimal for both SL (410±23 mg of gallic acid equivalent [GAE] per kg) and AW (666±63 mg GAE/kg). Both extracts exhibited chemical antioxidant activity and inhibited the protection of human plasma against chemically induced oxidation. SL extract did not exhibit free radical scavenging activity (DPPH). Importantly, no cytotoxicity (IC50>250 µg/mL) was observed in human-derived THP-1, HUVEC, HT-29, and HepG2 cell lines, ensuring the relative safety of these extracts for potential food technology applications. AW and SL extracts (100 µg/mL) reduced intracellular reactive oxygen species generation in lipopolysaccharide (LPS)-challenged THP-1 cells by 66% and 30%, respectively, highlighting the higher antioxidant activity of AW extract. AW and SL extracts did not modulate TNF-α and IL-6 secretion to show anti-inflammatory activity. Gummies infused with AW and SL extracts had a higher total phenolic content than commercial products, but their antioxidant activity was lower. Sensory evaluation revealed that gummies containing seaweed extracts received lower scores for colour, taste, overall impression, and purchase intention than those without extracts.
大西洋裙带菜(AW)和海莴苣(SL)是亚洲和欧洲盛产的食用海藻,通常作为新鲜或干燥的生物质食用。由于保质期较短,开发新的收获后处理方法(如提取生物活性化合物)对于建立可靠的大型海藻食品应用供应链至关重要。本研究旨在为功能性食品应用开发一种富含生物活性化合物的可持续功能性海藻食品配料。结果表明,使用 70% 乙醇超声辅助萃取 10 分钟对 SL(410±23 毫克没食子酸当量 [GAE]/千克)和 AW(666±63 毫克 GAE/千克)都是最佳的。两种提取物都具有化学抗氧化活性,并能抑制人体血浆的化学氧化。SL 提取物没有自由基清除活性(DPPH)。重要的是,在源自人类的 THP-1、HUVEC、HT-29 和 HepG2 细胞系中未观察到细胞毒性(IC50>250 µg/mL),确保了这些提取物在潜在食品技术应用中的相对安全性。AW和SL提取物(100微克/毫升)可使受到脂多糖(LPS)挑战的THP-1细胞的细胞内活性氧生成量分别减少66%和30%,这表明AW提取物具有更高的抗氧化活性。AW 和 SL 提取物没有调节 TNF-α 和 IL-6 的分泌,因此不具有抗炎活性。与商业产品相比,含 AW 和 SL 提取物的软糖的总酚类含量更高,但其抗氧化活性较低。感官评估显示,含有海藻提取物的软糖在颜色、味道、总体印象和购买意向方面的得分均低于不含提取物的软糖。
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引用次数: 0
Chemical, rheological and sensory characteristics of wheat bread enriched with chia (Salvia hispanica L.) seed gum 富含奇异籽胶(Salvia hispanica L.)的小麦面包的化学、流变和感官特性
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-09 DOI: 10.1016/j.fufo.2024.100471
Rawdah M. Al-Ali , Orass T. Al-Ibresam , Sawsan A. Al-Hilifi , Anka Trajkovska Petkoska , Sameh A. Korma
Bread is a staple food in many countries worldwide. The aim of this study is the presentation of a novel type of bread prepared of wheat flour and extract of chia seed gum (CSG). The CSG was included in the wheat flour mixture in a concentration from 2 to 10%. The flour mixture was characterized by its rheological properties through amylography, farinography, and extensography tests. The bread was also subjected to texture, color, and tactile analysis. The highest grades of the bread in terms of appearance, flavor, aroma, texture, and color were shown for flour mixture that contained 10% of CSG (p < 0.05); namely, this wheat mixture showed an improved value of volume, specific size, external and internal characteristics of bread.
面包是世界上许多国家的主食。本研究的目的是介绍一种由小麦粉和奇异籽胶(CSG)提取物制成的新型面包。在小麦粉混合物中加入的奇异籽胶浓度为 2% 至 10%。面粉混合物的流变特性通过淀粉图谱、淀粉图谱和延展图谱测试进行表征。此外,还对面包的质地、颜色和触感进行了分析。在外观、风味、香气、质地和颜色方面,含 10%南玻集团的面粉混合物的面包等级最高(p <0.05);也就是说,这种小麦混合物在面包的体积、比重大、外部和内部特性方面都有提高。
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引用次数: 0
Improving iron content in sustainable mycoprotein production through seawater fermentation 通过海水发酵提高可持续菌体蛋白生产中的铁含量
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-09 DOI: 10.1016/j.fufo.2024.100473
Bi-Hua Yang , Tzu-Chun Lin , Ting-Wei Lin , Shan Lin , I-Chen Li , Yen-Lien Chen , Tsung-Ju Li , Chin-Chu Chen
The growing global population and rising protein demand are straining freshwater resources. Fusarium venenatum (Fv) mycoprotein offers a sustainable protein alternative, with environmental efficiency and potential health benefits. However, its low iron content remains a concern, especially for vegetarians and vegans. This study introduces a sustainable approach, employing seawater as a fermentation medium for Fv production. Our analysis reveals that mycoprotein derived from SEA Fv exhibits elevated levels of sodium and calcium, with a notably high iron content (2.2 mg/100 g wet weight). The sodium content, while 3.31 times higher than in non-seawater fermentation, remains within recommended daily intake parameters. No plasticizers or heavy metals were detected in the SEA Fv cell body, minimizing long-term toxicity risks from seawater use. A unique metabolite, dihydroorotic acid, was identified from an in-house library of 774 metabolites, serving as an internal biomarker for seawater-based production methods. An acute safety study condensing 600 g of SEA Fv to simulate high mycoprotein digestion showed no effects on key physical behaviors or major organs, including the heart and lungs. This positions the product as a viable protein alternative with enhanced iron content, highlighting seawater-based fermentation as a sustainable method for future food production and industry progress.
全球人口的不断增长和对蛋白质需求的不断增加使淡水资源日趋紧张。Fusarium venenatum(Fv)菌体蛋白提供了一种可持续的蛋白质替代品,具有环境效益和潜在的健康益处。然而,它的低铁含量仍然是一个令人担忧的问题,尤其是对素食主义者来说。本研究介绍了一种可持续的方法,即利用海水作为发酵介质来生产 Fv。我们的分析表明,从 SEA Fv 提取的霉菌蛋白钠和钙含量较高,铁含量也很高(2.2 毫克/100 克湿重)。钠含量是非海水发酵的 3.31 倍,但仍在建议的每日摄入量范围内。SEA Fv 细胞体中未检测到增塑剂或重金属,最大程度地降低了使用海水的长期毒性风险。从内部的 774 种代谢物库中发现了一种独特的代谢物--二氢乳清酸,可作为基于海水的生产方法的内部生物标志物。一项急性安全研究将 600 克 SEA Fv 浓缩以模拟高霉菌蛋白消化,结果表明对关键的身体行为或主要器官(包括心脏和肺)没有影响。这将该产品定位为一种铁含量更高的可行蛋白质替代品,突出表明海水发酵是未来食品生产和工业进步的一种可持续方法。
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引用次数: 0
Fast and simultaneous prediction of inner quality parameters on intact mangos by near infrared spectroscopy: Impact of spectra pre-processing on prediction accuracy 利用近红外光谱快速、同步地预测完整芒果的内部质量参数:光谱预处理对预测准确性的影响
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-02 DOI: 10.1016/j.fufo.2024.100463
Agus Arip Munawar , Hizir , Cut Erika , Elke Pawelzik
Near infrared spectroscopy or known as NIRS has been widely employed in many fields including agriculture, especially for sorting and grading of agricultural products. Spectra pre-processing is one of the main factors affecting model accuracy and prediction capabilities of NIRS. The objective of the present study was to study the impact of different spectra corrections namely mean centering (MC), mean normalization (MN), de-trending (DT), multiplicative scatter correction (MSC), standard normal variate (SNV) and orthogonal signal correction (OSC), to the prediction accuracy of quality parameters: titratable acidity (TA) and soluble solids content (SSC) in intact mango. A total of 91 mango samples (cv. Kent) were used as dataset for calibration and external prediction which was separated by means of systematic sampling based on a property (SSBP) approach. Diffuse reflectance spectra (log1/R) were acquired and recorded in wavelength range of 1000 – 2500 nm by Antaris Fourier transform NIR instrument. Judging from calibration and prediction performance, MSC found to be the best spectra pre-processing method prior to prediction model development with R2 prediction are 0.72 for TA and 0.76 for SSC. Although MSC increase the prediction performances based on R2, RMSE, RPD and RER metrics compared to the baseline, the achieved RPD, 1.9 for TA and 1.8 for SSC of this findings are still poor and need improvements to achieve even higher levels of accuracy and reliability necessitates for real-time applications.
近红外光谱(NIRS)已被广泛应用于包括农业在内的许多领域,特别是农产品的分类和分级。光谱预处理是影响近红外光谱仪模型准确性和预测能力的主要因素之一。本研究的目的是研究不同光谱校正(即平均居中(MC)、平均归一化(MN)、去趋势(DT)、乘法散度校正(MSC)、标准正态变异(SNV)和正交信号校正(OSC))对完整芒果中可滴定酸度(TA)和可溶性固形物含量(SSC)等质量参数预测准确性的影响。共使用了 91 个芒果样品(品种:肯特)作为校准和外部预测的数据集,这些数据集是通过基于属性的系统取样(SSBP)方法分离出来的。利用 Antaris 傅立叶变换近红外仪器采集并记录了波长范围为 1000 - 2500 nm 的漫反射光谱(log1/R)。从校准和预测性能来看,MSC 是建立预测模型前的最佳光谱预处理方法,对 TA 的 R2 预测值为 0.72,对 SSC 的 R2 预测值为 0.76。虽然与基线相比,基于 R2、RMSE、RPD 和 RER 指标,MSC 提高了预测性能,但这一研究结果的 RPD(TA 为 1.9,SSC 为 1.8)仍然较差,需要改进才能达到实时应用所需的更高准确度和可靠性。
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引用次数: 0
Towards the expansion of the functional dairy market: Determining donkey milk value propositions and identifying possible consumers 拓展功能性乳品市场:确定驴奶的价值主张并确定可能的消费者
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-01 DOI: 10.1016/j.fufo.2024.100467
B. Keitshweditse , C.Z. Tsvakirai , M.L. Mabuza , M. Tshehla
The demand for donkey milk has increased due to consumers’ growing preference for functional dairy beverages. As the commercial value of donkey milk and its marketable qualities still need to be discovered by many consumers, information on how to market this functional dairy product is needed. Hence, this study aimed to determine marketable value propositions, competitive pricing levels for donkey milk, and possible early adopters. Using data collected in the Mahikeng Local Municipality in South Africa, consumers’ willingness to pay for donkey milk was determined, and a double-bound probit model was utilized to determine socio-economic characteristics that could serve as a target market for donkey milk. The study found that consumers were willing to pay a 10 % premium for donkey milk, a lower percentage than premiums reported in other parts of the world where donkey milk has been commercialized. The study's findings revealed that the marketing of donkey milk should target the wealthy and young people in South Africa. However, the industry's growth shall likely be stalled by the current heritage value proposition it is known for, which attracts a relatively small premium. This study recommends that consumers be furnished with scientific evidence that substantiates the nutraceutical properties of donkey milk so that this becomes an equally known value proposition that results in a higher premium, as reported in other developed markets.
由于消费者越来越青睐功能性乳饮料,对驴奶的需求也随之增加。由于驴奶的商业价值及其适销性仍有待许多消费者发掘,因此需要有关如何营销这种功能性乳制品的信息。因此,本研究旨在确定驴奶的市场价值主张、有竞争力的定价水平以及可能的早期采用者。利用在南非马希肯地方市收集的数据,确定了消费者对驴奶的支付意愿,并利用双约束 probit 模型确定了可作为驴奶目标市场的社会经济特征。研究发现,消费者愿意为驴奶支付 10% 的溢价,这一比例低于世界其他驴奶商业化地区所报告的溢价比例。研究结果表明,驴奶的营销应以南非的富人和年轻人为目标。然而,驴奶产业的发展很可能会因为其目前的传统价值主张而停滞不前,因为这种价值主张所吸引的溢价相对较小。本研究建议向消费者提供科学证据,证实驴奶的营养保健特性,使其成为一个同样广为人知的价值主张,从而获得更高的溢价,正如其他发达市场所报告的那样。
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引用次数: 0
Sustainable materials for novel food packaging based on alginate, methylcellulose and glycerol films functionalized with gallic acid 基于没食子酸功能化海藻酸盐、甲基纤维素和甘油薄膜的新型食品包装可持续材料
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-30 DOI: 10.1016/j.fufo.2024.100464
Francisco Javier Leyva-Jiménez , Carlos Abellán-Dieguez , Rodrigo Oliver-Simancas , Antonio M. Rodríguez-García , M. Elena Alañón
In the last years, new sustainable materials have been explored as plastic replacement in food industry. With the objective of developing sustainable active materials for food packaging, polysaccharide-based films: alginate, methylcellulose and glycerol-based films (ALG-MC-GLY (65:30:5)) were formulated and functionalized with different concentration of gallic acid (0.1, 0.5 and 0.8 % w/v). The mechanical, physicochemical, optical and functional properties of the films were evaluated. The incorporation of gallic acid resulted in films less resistant to tensile stress and less elastic, exhibiting reduced water vapor permeability. The physico-mechanical characterization was corroborated by FTIR spectra, HRSEM imaging and BET analyses, which demonstrated the interactions between the polymeric chains and gallic acid through localized hydrogen bonding, leading to denser structures with increased brittleness at higher concentrations of gallic acid. Moreover, the films functionalized with gallic acid displayed enhanced antioxidant properties in a concentration-dependent manner. These findings suggest that the ALG-MC-GLY based films loaded with gallic acid hold significant promise as alternative active food packaging materials.
近年来,人们一直在探索新的可持续材料,以替代食品工业中的塑料。为了开发用于食品包装的可持续活性材料,我们配制了多糖基薄膜:海藻酸盐、甲基纤维素和甘油基薄膜(ALG-MC-GLY (65:30:5)),并用不同浓度的没食子酸(0.1%、0.5% 和 0.8% w/v)进行功能化。对薄膜的机械、物理化学、光学和功能特性进行了评估。加入没食子酸后,薄膜的抗拉伸应力和弹性降低,水蒸气渗透性降低。傅立叶变换红外光谱、HRSEM 成像和 BET 分析证实了物理机械特性,证明了聚合物链与没食子酸之间通过局部氢键相互作用,导致结构更致密,没食子酸浓度越高,脆性越大。此外,用没食子酸功能化的薄膜显示出更强的抗氧化性,其程度与浓度有关。这些研究结果表明,基于 ALG-MC-GLY 的含没食子酸薄膜有望成为替代性活性食品包装材料。
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引用次数: 0
Plant-based biomaterials as scaffolds for cellular agriculture 作为细胞农业支架的植物基生物材料
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-30 DOI: 10.1016/j.fufo.2024.100468
Woo-Ju Kim , Yoonbin Kim , Reza Ovissipour , Nitin Nitin
Edible scaffolds are the essential components for cultivated meat. This research aimed to evaluate the performance of food-grade polysaccharides (pectin and alginate) and proteins (soy protein isolate (SPI) and pea protein isolate (PPI)) as scaffolds for cultivated meat production. A myoblast model cell line (C2C12) and an embryonic-derived fish cell line (ZEM2S) were selected as model cell lines. Rheological analysis revealed that the selected gels were viscoelastic solids with shear thinning behavior. The storage modulus (G') and loss modulus (G'') of pectin and PPI were greater than 1000 Pa and 100 Pa. The pectin gels exhibit better structure recovery compared to other biopolymer gels. The texture properties were similar to those of commercial meat analogues. Furthermore, the pectin gels were stable in water. In general, the cytocompatibility of the biomaterial gels was similar for the tested cell lines, except for ZEM2S when exposed to protein gels. Based on the results of mechanical properties and cytocompatibilities of gels, a 3D printed structure with pectin gel was generated. 3D-printed scaffolds promoted the proliferation of C2C12 cells during 5 days of incubation. These findings highlight the potential of plant biomaterials and 3D printing to develop scaffolds for the production of cultivated meat.
可食用支架是培育肉类的重要成分。本研究旨在评估食品级多糖(果胶和海藻酸)和蛋白质(大豆分离蛋白(SPI)和豌豆分离蛋白(PPI))作为培养肉生产支架的性能。选择了一种肌母细胞模型细胞系(C2C12)和一种胚胎衍生鱼细胞系(ZEM2S)作为模型细胞系。流变学分析表明,所选凝胶是具有剪切稀化行为的粘弹性固体。果胶和 PPI 的储存模量(G')和损耗模量(G'')分别大于 1000 Pa 和 100 Pa。与其他生物聚合物凝胶相比,果胶凝胶具有更好的结构恢复性。其质地特性与商用肉类类似物相似。此外,果胶凝胶在水中也很稳定。总的来说,生物材料凝胶的细胞相容性与测试的细胞系相似,但 ZEM2S 在接触蛋白质凝胶时除外。根据凝胶的机械性能和细胞相容性结果,生成了含有果胶凝胶的三维打印结构。在 5 天的培养过程中,三维打印支架促进了 C2C12 细胞的增殖。这些发现凸显了植物生物材料和三维打印技术在开发培养肉生产支架方面的潜力。
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引用次数: 0
期刊
Future Foods
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