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Egyptian Academic Journal of Biological Sciences B Zoology最新文献

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‌Prioritizing and Evaluating Conservation Areas: A case for the Threatened Ecosystem Engineer the Egyptian Dabb lizard Uromastyx aegyptia in the Eastern Desert of Egypt. 优先考虑和评估保护区:一个受威胁的生态系统工程师的案例埃及东部沙漠中的埃及大蜥蜴。
Pub Date : 2022-06-29 DOI: 10.21608/eajbsz.2022.246343
Abdullah Nagy, Eman M. E. Mohallal, S. El-Kafrawy, S. Saber
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引用次数: 0
Role of Stem Cells and Erythropoietin on Experimental Acute Hepatic Injury in Rats 干细胞和促红细胞生成素在大鼠实验性急性肝损伤中的作用
Pub Date : 2022-05-20 DOI: 10.21608/eajbsz.2022.238371
Samir A. M. Zaahkouk, M. A, Ibrahim Hassan, S. El-Shamy
Erythropoietin (EPO) exhibits nonhematopoietic functions. Besides its hematopoietic functions, EPO has anti-apoptotic, anti-oxidant and anti-inflammatory activities. Tissue-protective EPO effect was confirmed in brain, myocardium, liver, and kidney injury. Mesenchymal stem cells (MSCs) are nonhematopoietic cells. It may be extracted from the bone marrow, dental pulp, adipose tissue, placenta, umbilical cord, or amniotic membrane. Pre-clinical and clinical evidence shows that MSCs derived can heal injured liver tissues, enhance liver functions, and reduce liver fibrosis. Material and Methods : Hundred male albino rats were classified into five groups equally. Some biochemical and Immunohistochemical studies of COX-2 were assessed in the liver tissue. Results: MSCs and EPO treatment ameliorated the toxic effect of GalN with a significant decrease in the mean level of ALAT, ASAT, and ammonia. The combination between MSCs and EPO showed more effective protection against GalN toxicity.
促红细胞生成素(EPO)具有非造血功能。EPO除具有造血功能外,还具有抗凋亡、抗氧化和抗炎活性。在脑、心肌、肝、肾损伤中证实了EPO的组织保护作用。间充质干细胞(MSCs)是非造血细胞。它可以从骨髓、牙髓、脂肪组织、胎盘、脐带或羊膜中提取。临床前和临床证据表明,MSCs可以愈合损伤的肝组织,增强肝功能,减少肝纤维化。材料与方法:雄性白化大鼠100只,随机分为5组。在肝组织中进行COX-2的生化和免疫组织化学研究。结果:骨髓间充质干细胞和EPO治疗可改善GalN的毒性作用,显著降低ALAT、ASAT和氨的平均水平。骨髓间充质干细胞与EPO联合对GalN毒性的保护作用更强。
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引用次数: 0
Biodiversity and Distribution of Lizards in Jazan area, Kingdom of Saudi Arabia 沙特阿拉伯王国吉赞地区蜥蜴的生物多样性和分布
Pub Date : 2022-05-18 DOI: 10.21608/eajbsz.2022.237396
Yatimi, Khayrah, Fathy, M.
Background: Jazan region is one of the most important areas of the Kingdom that is located in the southwest of the country. It is considered one of the richest areas in biodiversity and it encompasses a large group of lizards belonging to different families. The aim of the current research is to survey different types of lizards in Jazan. Materials and Methods: Lizards were collected from Al-Aradha, AL-tuwal and Damad by hand capturing between dawn and midmorning, or shortly before sunset. Results: In this work, eight species belonging to four families (Camaeleonidae, Scincidae, Gekkonidae, and Agamidae) were collected and identified. Most of the gathered species belong to the family Gekkonidae. Conclusions: The widespread of species belonging to the family Gekkonidae may be attributed to the ability of these species to withstand various climatic conditions and to live in different habitats using the available facilities to survive and broadly distribute.
背景:吉赞地区是王国最重要的地区之一,位于该国西南部。它被认为是生物多样性最丰富的地区之一,它包含了属于不同科的一大群蜥蜴。目前的研究目的是调查吉赞不同类型的蜥蜴。材料和方法:在黎明至中午或日落前不久,通过手工捕获在Al-Aradha, AL-tuwal和Damad采集蜥蜴。结果:共采集鉴定出4科8种,分别为山虻科、山虻科、山虻科、山虻科。大多数收集到的物种属于壁虎科。结论:该科物种分布广泛,可能是由于它们能够适应各种气候条件,利用现有设施在不同的生境生存和广泛分布。
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引用次数: 0
The Protective Effect of Moringa Oleifera Seeds Extract on Liver Damage in Mice 辣木籽提取物对小鼠肝损伤的保护作用
Pub Date : 2022-04-20 DOI: 10.21608/eajbsz.2022.233847
Athraa H. Ali
The current study to assess the antioxidant and hepatoprotective properties of Moringa oleifera seed extract versus carbon tetrachloride (CCl4)-stimulated liver damage. Materials &Methods: Male albino mice were separated into four groups for the investigation The control group (A) was given distilled water (DW), whereas the other three groups (B, C, and D) were administered CCl4 intraperitoneally (IP) (0.5 ml/kg) mixed with corn oil (vol/vol), for 8 weeks, twice a week. Group (C) was given Moringa seed extract (1g/kg) orally every day for 8 weeks, while group (D) was given corn oil orally, and the groups (C) and (D) were compared to group (B) which was given CCl4 IP. The researchers measured liver functions, blood-reduced glutathione (GSH), total antioxidant capacity (TAC), and malondialdehyde (MDA) in erythrocytes. Moreover, catalase enzymes in liver tissue homogenate were also assayed and superoxide dismutase (SOD), in addition to liver histopathology. Results : Following a CCl4 injection, liver enzymes and MDA levels were elevated. Conversely, TAC, serum albumin, total protein, catalase, tissue SOD, and blood glutathione levels were all decreased. Administration to Moringa oleifera seeds extract reduces MDA levels and enzyme activity in the liver while increasing antioxidant activity. Simultaneously, after treatments with this extract, the histological damage effects of CCl4 intoxication were also improved. Conclusion: Finally, Moringa oleifera seeds extract increased innate antioxidant activity and reduced CCl4-induced liver damage, suggesting that it might be employed as a hepatoprotective medication in the future.
本研究旨在评估辣木籽提取物对四氯化碳(CCl4)刺激的肝损伤的抗氧化和肝保护作用。材料与方法:将雄性白化小鼠分为4组进行研究,对照组(A组)给予蒸馏水(DW),其余3组(B、C、D组)给予CCl4 (0.5 ml/kg)与玉米油(vol/vol)混合腹腔注射,连续8周,每周2次。(C)组每天口服辣木籽提取物(1g/kg),连续8周,(D)组口服玉米油,(C)组和(D)组与(B)组比较,(B)组给予CCl4 IP。研究人员测量了红细胞中的肝功能、血还原型谷胱甘肽(GSH)、总抗氧化能力(TAC)和丙二醛(MDA)。此外,检测肝组织匀浆过氧化氢酶和超氧化物歧化酶(SOD),以及肝脏组织病理学。结果:注射CCl4后,肝酶和MDA水平升高。相反,TAC、血清白蛋白、总蛋白、过氧化氢酶、组织SOD和血谷胱甘肽水平均下降。给药辣木籽提取物降低丙二醛水平和肝脏酶活性,同时增加抗氧化活性。同时,经该提取物处理后,CCl4中毒的组织学损伤作用也得到改善。结论:辣木籽提取物具有增强天然抗氧化活性和减轻ccl4诱导的肝损伤的作用,提示辣木籽提取物可能在未来作为肝保护药物使用。
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引用次数: 0
Breast Cancer: Hormonal Changes, Symptoms, Treatment and Factors Acting During Intrauterine Life. 乳腺癌:激素变化、症状、治疗和宫内生活中的因素。
Pub Date : 2022-04-18 DOI: 10.21608/eajbsz.2022.233716
S. Ibraheem, Z. Hassan
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引用次数: 0
Thyroid Hormone Concentration and Receptor 甲状腺激素浓度和受体
Pub Date : 2022-04-17 DOI: 10.21608/eajbsz.2022.233650
N. Ali, Amir A. Majeed
Hormones are hormones secreted by the thyroid gland and their primary function is to regulate the speed of metabolism in many tissues. The thyroid gland releases its hormones into the bloodstream in response to a regulating hormone released by the pituitary gland called thyroid-stimulating hormone. It is the only organ in the body that has the ability to synthesize iodine in large quantities for use in the synthesis of thyroid hormones. It consists of two lobes, joined together by the isthmus, located above the windpipe, just below the cartilage, which is the marker for its location. The weight of the thyroid glands in humans ranges from 15-to 20 grams and the blood supply changes depending on the functioning of the gland. There are lymphatic vessels, but their function in relation to endocrine function is uncertain.
激素是由甲状腺分泌的激素,其主要功能是调节许多组织的代谢速度。甲状腺释放激素到血液中,以响应脑垂体释放的一种调节激素,即促甲状腺激素。它是人体中唯一能够大量合成碘以用于甲状腺激素合成的器官。它由两个肺叶组成,由峡部连接在一起,位于气管上方,就在软骨下方,这是它的位置标记。人体甲状腺的重量在15- 20克之间,血液供应随甲状腺功能的变化而变化。有淋巴管,但其功能与内分泌功能的关系尚不清楚。
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引用次数: 1
Influence of Scorpion Venom on Decomposition and Arthropod Succession . 蝎子毒液对分解和节肢动物演替的影响。
Pub Date : 2022-04-16 DOI: 10.21608/eajbsz.2022.231536
F. A. Abd El-Aziz, S. Eldeeb, Nora Z. Abdellah, Eman S. Shaltout, N. Ebrahem
Background: The contributions of forensic entomology and arthropod in legal investigations have been known for centuries. There are many ways that insects can be used to help in solving a crime, but the primary purpose of forensic entomology is the determination of postmortem interval. The aim of this work is to evaluate the effect of scorpion venom on the decomposition of rabbits’ carcasses and on arthropods’ colonization of rabbits’ carcasses in indoor and outdoor environments. Methods: Twenty male rabbits were included in the study, divided into four groups, with 5 rabbits in each group. two groups were killed by spinal cord separation; group (1) kept in an indoor environment and group (3) in an outdoor environment. The other two groups were killed by scorpion stings (groups 2 and 4), kept indoors, and outdoor respectively. The carcasses were allowed to decompose, and arthropods were collected daily and examined morphologically. Results: The decomposition findings were less prominent in groups 2 and 4 with less maggot mass compared to the control groups. Furthermore, arthropod species were different and showed morphological changes in the form of dryness with the formation of ulcers in the larvae, appearance of irregular groves and tunnels in insects and dryness of the outer layer with damage to the ends of beetles. Conclusion: Scorpion venom affected the decomposition rate of rabbits’ carcasses and arthropods’ colonization with the difference in morphological appearance of arthropods between the control groups and scorpion stinging groups, which may be of forensic importance.
背景:几个世纪以来,法医昆虫学和节肢动物在法律调查中的贡献一直为人所知。有很多方法可以利用昆虫来帮助破案,但法医昆虫学的主要目的是确定死后时间。本研究的目的是评价蝎子毒液对家兔尸体在室内和室外环境中的分解和节肢动物在家兔尸体上定植的影响。方法:选取雄性家兔20只,随机分为4组,每组5只。脊髓分离处死两组;组(1)为室内环境,组(3)为室外环境。另外2组(2组和4组)用蝎子螫杀,分别饲养在室内和室外。让尸体腐烂,每天收集节肢动物并进行形态学检查。结果:与对照组相比,2组和4组的分解结果不明显,蛆质量较小。此外,节肢动物种类不同,表现出干燥的形态变化,幼虫形成溃疡,昆虫出现不规则的小林和隧道,甲虫外层干燥,末端受损。结论:蝎子毒液影响了家兔尸体的分解率和节肢动物的定植,且节肢动物的形态外观在对照组和蝎子蜇伤组之间存在差异,这可能具有重要的法医学意义。
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引用次数: 0
Biochemical, Molecular and Histopathological Studies on Malathion Toxicity on Some Vital Organs of Male Rats 马拉硫磷对雄性大鼠重要器官毒性的生化、分子和组织病理学研究
Pub Date : 2022-04-16 DOI: 10.21608/eajbsz.2022.231218
Nourhan Morsi, Mohamed Wassem, M. Badr, A. K. A. Abdel Latif
pesticide widely used throughout agriculture and veterinary practices. The present study aimed to evaluate the toxic effects of Malathion inhalation through simulation of three models of environmental toxicity in different exposure periods of acute, chronic, and subchronic doses. The histological, immunohistochemical examinations of the morphological integrity of the liver and brain, together with some biochemical (TAC, CAT, SOD, GPx and Se) and molecular (Real-time PCR for p53 and P21 mRNA) studies will be assessed to highlight the effects of the studied sub-lethal doses of Malathion. Four groups of male Wister rats were used in the experiment one for the control group and the others for subchronic, chronic and acute doses. Many toxic symptoms, a significant decrease in CAT, CAT, SOD, GPx and Se values. Malathion cause also significant disturbance in different comet assay parameters in both blood and liver tissue cells. Also, Real-time PCR for mRNA of P53 and P21 in the liver showed a significant increase in mRNA expression of P53 and P21 and a significant decrease in mRNA expression of P53 and P21 in brain tissue. P53 immunohistochemistry in liver tissue shows an increase in its activity and a decrease in hippocampus tissue cells. Many histopathological lesions such as inflammation, vacuolation, apoptosis, necrosis, and fibrosis of the hepatic tissue cells, were recorded in all treated groups.
农药广泛应用于农业和兽医实践。本研究旨在通过模拟急性、慢性和亚慢性剂量马拉硫磷不同暴露期的三种环境毒性模型来评估吸入马拉硫磷的毒性作用。将通过组织病理学、免疫组织化学检查肝脏和大脑的形态学完整性,以及一些生化(TAC、CAT、SOD、GPx和Se)和分子(实时荧光定量PCR检测p53和P21 mRNA)研究来评估马拉硫磷亚致死剂量的影响。实验采用雄性Wister大鼠四组,分别为对照组、亚慢性、慢性和急性剂量组。许多中毒症状,CAT、CAT、SOD、GPx和Se值显著降低。马拉硫磷对血液和肝组织细胞的不同彗星测定参数也有显著的干扰。肝脏中P53和P21 mRNA的Real-time PCR显示,P53和P21 mRNA表达量显著升高,脑组织中P53和P21 mRNA表达量显著降低。肝组织中P53免疫组化显示其活性增加,海马组织细胞中P53活性降低。所有治疗组肝脏组织细胞均出现炎症、空泡、细胞凋亡、坏死、纤维化等病理变化。
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引用次数: 0
Suppress Root-Knot Nematode Infested Vegetable Fields Via Enhancing Rhizobacteria Inoculated in Composted Chicken Manure 鸡粪堆肥中接种根瘤菌抑制菜田根结线虫
Pub Date : 2022-04-06 DOI: 10.21608/eajbsz.2022.229451
E. Mostafa, R. El-Ashry, Abdelhadi A I Ali
organic manures us to focus on their exploration in agriculture. Laboratory effectiveness of Serratia spp. and Pseudomonas spp. isolates against egg hatching and infective juvenile mortality of the root-knot nematode, Meloidogyne incognita was determined. As well as performance evaluation of composted chicken manure (CM) inoculated with bacteria under greenhouse and field conditions on vegetable plants, cucumber, pepper, potato and tomato besides In vitro results after the 5 th day of inoculation, S. marcescens (A10) and Pseudomonas fluorescens PF131 isolates gained the highest inhibition of egg hatching (85.18 and 75.36%). While juvenile mortality percentages were 43.30 and 35.10% with S. marcescens (A10) and P. putida (PP29), respectively. The nematicidal effect of the tested rhizobacteria on M. incognita inhibited egg hatching and juvenile mortality was directly proportional to isolates and exposure time. Under greenhouse conditions, the interaction between rhizobacteria and CM increased the fresh potato plant's shoot weight (31.78%) and root weight (28.94 %). Furthermore, the decrease in the number of galls, egg masses, IJs/100 g soil, and RF population significantly to 69.57%, 63.37%, 12.40, and 0.147, respectively. Under field conditions, the combination of chicken manure seems to be responsible for the sustainability of rhizobacteria in situ and extends their potency for a long period. After 20 days of the field application (1.8×10 8 cfu/ml; 15 L /Fed), the reduction percentages in IJs in blocks treated with the mixture of rhizobacteria and were 88.94, 87.92, 91.27, and 9276 % in tomato, cucumber, pepper, and banana plants, respectively. Our results indicate that the chicken manure inoculated with rhizobacteria is a promising biocontrol agent M. incognita
有机肥料是我们在农业上的重点探索。研究了分离的沙雷氏菌和假单胞菌对根结线虫虫卵孵化和幼虫感染性死亡的室内效果。对鸡粪堆肥(CM)在温室和田间条件下对蔬菜、黄瓜、辣椒、马铃薯和番茄的孵化性能进行了评价。体外试验结果表明,接种第5 d后,粘质葡萄球菌(S. marcescens, A10)和荧光假单胞菌(Pseudomonas fluorescens, PF131)的抑卵率最高,分别为85.18%和75.36%。粘质S. marcescens (A10)和恶臭P. putida (PP29)的幼虫死亡率分别为43.30%和35.10%。所试根杆菌的杀线虫作用与菌株和暴露时间成正比。在温室条件下,根瘤菌与CM的交互作用使鲜马铃薯植株的茎重和根重分别增加了31.78%和28.94%。虫瘿数、卵块数、ij /100 g土壤和RF种群数量显著减少,分别为69.57%、63.37%、12.40和0.147。在田间条件下,鸡粪的组合似乎对根杆菌在原位的可持续性负责,并延长其效力很长一段时间。现场施用20天后(1.8×10 8 cfu/ml;15 L /Fed)处理后,番茄、黄瓜、辣椒和香蕉的IJs降低率分别为88.94%、87.92%、91.27%和9276%。结果表明,鸡粪中接种根瘤菌是一种很有前途的生物防治剂
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引用次数: 1
Stress Responses of Red Tilapia (Oreochromis Spp.) Exposed to Blue and Red-Light Emitting Diode (Led) 红罗非鱼(Oreochromis Spp.)对蓝光和红光发光二极管(Led)的应激反应
Pub Date : 2022-04-01 DOI: 10.21608/eajbsz.2022.228679
Raffy Apino, Rea Mae emplonuevo, E. V. Vera Cruz
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引用次数: 1
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Egyptian Academic Journal of Biological Sciences B Zoology
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