Pub Date : 2023-08-07DOI: 10.56557/pcbmb/2023/v24i3-48336
S. Shahin, R. Said, Abla H. Dergham
Under screen house at the nursery of Hort. Res. Inst., A.R.C., Giza Egypt, a pot experiment was conducted during 2021 seasons to study the response of Taiwan sugar palm (Arenga engleri Bcc.) seedlings to planting in the different growing media (sand, clay and sand + clay at 1: 1, v/v), different irrigation treatments (100, 75 and 50 % of F.C. of each medium) and their interactions in a factorial experiment arranged in completely randomized design with three replications. Results of the experiment have shown that planting in sand + clay mixture (1:1, v/v) gave the highest mean values of different growth parameters, with few exceptions as compared to planting either in sand alone or clay standalone in the two seasons. In addition, the gradual decrement in water supply was accompanied by a gradual decrease in values of all vegetative and root growth traits to reach minimum by 50 % of F.C. water treatment in both seasons. However, the reduction percentage caused by 75 % of F.C. treatment ranged between 1.1 to 17.6 % for different growth characters, while that induced by 50 % of F.C. one ranged between 23.8 to 40.4 %. The growth performance of plants watered with 75 % F.C. was greatly resemble to that of plants watered with 100 % F.C., whereas that of plants watered with 50 % F.C. was not, as it gave the smallest plants, which can be used for decorating the limited area places. The treatments showed a marked influence on various growth traits, but the dominance was for the combining between planting in sand + clay mixture and irrigating with 100 % F.C. water treatment, as such combination recorded the utmost high means in different growth traits over the other combinations, with few exceptions in both seasons. A similar response occurred, concentrations of chlorophyll a, b, carotenoids, total carbohydrates, N, P and K in the leaves. Accordingly, it can be recommended to planting arenga sugar palm seedlings in sand + clay mixture at equal volumetric parts and irrigating them with 100 %, 75 % or 50 % of F.C. water treatments to produce different sizes from such ornamental palm as pot plants suitable for landscaping the limited places of different areas.
{"title":"Effect of Irrigation Water Treatments on Growth and Quality of Taiwan Sugar Palm Seedlings Grown in Some Traditional Growing Media","authors":"S. Shahin, R. Said, Abla H. Dergham","doi":"10.56557/pcbmb/2023/v24i3-48336","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48336","url":null,"abstract":"Under screen house at the nursery of Hort. Res. Inst., A.R.C., Giza Egypt, a pot experiment was conducted during 2021 seasons to study the response of Taiwan sugar palm (Arenga engleri Bcc.) seedlings to planting in the different growing media (sand, clay and sand + clay at 1: 1, v/v), different irrigation treatments (100, 75 and 50 % of F.C. of each medium) and their interactions in a factorial experiment arranged in completely randomized design with three replications. \u0000Results of the experiment have shown that planting in sand + clay mixture (1:1, v/v) gave the highest mean values of different growth parameters, with few exceptions as compared to planting either in sand alone or clay standalone in the two seasons. In addition, the gradual decrement in water supply was accompanied by a gradual decrease in values of all vegetative and root growth traits to reach minimum by 50 % of F.C. water treatment in both seasons. However, the reduction percentage caused by 75 % of F.C. treatment ranged between 1.1 to 17.6 % for different growth characters, while that induced by 50 % of F.C. one ranged between 23.8 to 40.4 %. The growth performance of plants watered with 75 % F.C. was greatly resemble to that of plants watered with 100 % F.C., whereas that of plants watered with 50 % F.C. was not, as it gave the smallest plants, which can be used for decorating the limited area places. The treatments showed a marked influence on various growth traits, but the dominance was for the combining between planting in sand + clay mixture and irrigating with 100 % F.C. water treatment, as such combination recorded the utmost high means in different growth traits over the other combinations, with few exceptions in both seasons. A similar response occurred, concentrations of chlorophyll a, b, carotenoids, total carbohydrates, N, P and K in the leaves. \u0000Accordingly, it can be recommended to planting arenga sugar palm seedlings in sand + clay mixture at equal volumetric parts and irrigating them with 100 %, 75 % or 50 % of F.C. water treatments to produce different sizes from such ornamental palm as pot plants suitable for landscaping the limited places of different areas.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42043307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-03DOI: 10.56557/pcbmb/2023/v24i3-48330
F. Chamandoosti
The study was carried out in soil born diseases laboratory, Iranian Research Institute of Plant Protection during Mar 2022 to Sep 2022. The Single node explants (prepared from young certificated scions from Fadak garden in Qom province) of Koroneiki olive scions cultured on DKW medium. Culture media were free from PGRs in establishment stage. After the mentioned stage, explant cultured on DKW media fortified with PGRs, in order to presentation a simple and repeatable method for shoot multiplication which is one of the important method for In Vitro olive propagation by examining three item included, the mean shoot length, the mean shoot number and the mean leaf number per explants. PGRs included 1 – 4 mg/l ZEA, KIN, 2ip, TDZ and BA. ZEA and KIN to be superior to other cytokinin in terms of mean shoot length and mean shoot numbers, respectively. All of investigated item especially the mean shoot number and the mean shoot length increased when the concentrations of PGRs increased. The influence of other cytokines (2ip, BA and TDZ) on three above investigated items was different. Supplemented DKW media with 4 mg/l 2ip resulted the maximum number leaf per explants. Callusing and low growth of micro shoots on explants were the dominant responses on DKW media plus 3 mg/l BA. In addition, few weak micro shoots regenerated on DKW media with 4 mg/l TDZ.
{"title":"Effect of Cytokinins on the Vegetative Propagation of Olea europaea L. (cv. Koroneiki)","authors":"F. Chamandoosti","doi":"10.56557/pcbmb/2023/v24i3-48330","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48330","url":null,"abstract":"The study was carried out in soil born diseases laboratory, Iranian Research Institute of Plant Protection during Mar 2022 to Sep 2022. The Single node explants (prepared from young certificated scions from Fadak garden in Qom province) of Koroneiki olive scions cultured on DKW medium. Culture media were free from PGRs in establishment stage. After the mentioned stage, explant cultured on DKW media fortified with PGRs, in order to presentation a simple and repeatable method for shoot multiplication which is one of the important method for In Vitro olive propagation by examining three item included, the mean shoot length, the mean shoot number and the mean leaf number per explants. PGRs included 1 – 4 mg/l ZEA, KIN, 2ip, TDZ and BA. ZEA and KIN to be superior to other cytokinin in terms of mean shoot length and mean shoot numbers, respectively. All of investigated item especially the mean shoot number and the mean shoot length increased when the concentrations of PGRs increased. The influence of other cytokines (2ip, BA and TDZ) on three above investigated items was different. Supplemented DKW media with 4 mg/l 2ip resulted the maximum number leaf per explants. Callusing and low growth of micro shoots on explants were the dominant responses on DKW media plus 3 mg/l BA. In addition, few weak micro shoots regenerated on DKW media with 4 mg/l TDZ.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44747601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-12DOI: 10.56557/pcbmb/2023/v24i3-48311
D. Rasmia, Mohamed, A. M., M. Mervat, Al-Aramany, W. El Deen, H. Rabab
Barhee trees were facing the major problem as poor fruit set and low fruits quality (Shees fruits) and the low yield due to inadequate pollination, The date palm female trees under experimental study were uniformed under farm management as irrigation, pollination, pruning and soil fertilization with both mineral (N, P, K and S) and organic fertilizers (animal manure) to investigate the influence of boron and zinc to increasing fruit set and yield, zinc 1,2 and 3 g/l and Boron at 1,2 and 3 ml/l as foliar before pollinated inflorescences and soil application at 10,20 and 30 g/tree two times /month. The obtained results revealed that treated trees with Borax spraying at 2 ml/l + Zn 2 g/l as pre pollination with soil application at 20 ml Borax + 20 g/l Zn induced heavy fruit, flesh and seeds with longest fruits that contributed to the biggest bunch weight and total yield/ tree in addition to higher rates of fruit retention and Flesh fruit weight %, fruit acidity and phenols were reduced. So based on the previous results, spraying inflorescences and soil treatments with Borax and Zn were applied for increasing and enhancing fruit quality and maximum yield of date palm cv. Barhee.
{"title":"Zinc and Boron Application Affected a-Fruit Set and Fruit Quality of Date Palm cv. Barhee","authors":"D. Rasmia, Mohamed, A. M., M. Mervat, Al-Aramany, W. El Deen, H. Rabab","doi":"10.56557/pcbmb/2023/v24i3-48311","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48311","url":null,"abstract":"Barhee trees were facing the major problem as poor fruit set and low fruits quality (Shees fruits) and the low yield due to inadequate pollination, The date palm female trees under experimental study were uniformed under farm management as irrigation, pollination, pruning and soil fertilization with both mineral (N, P, K and S) and organic fertilizers (animal manure) to investigate the influence of boron and zinc to increasing fruit set and yield, zinc 1,2 and 3 g/l and Boron at 1,2 and 3 ml/l as foliar before pollinated inflorescences and soil application at 10,20 and 30 g/tree two times /month. The obtained results revealed that treated trees with Borax spraying at 2 ml/l + Zn 2 g/l as pre pollination with soil application at 20 ml Borax + 20 g/l Zn induced heavy fruit, flesh and seeds with longest fruits that contributed to the biggest bunch weight and total yield/ tree in addition to higher rates of fruit retention and Flesh fruit weight %, fruit acidity and phenols were reduced. So based on the previous results, spraying inflorescences and soil treatments with Borax and Zn were applied for increasing and enhancing fruit quality and maximum yield of date palm cv. Barhee.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42942659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.56557/pcbmb/2023/v24i3-48292
M. Rehman, M. Chaudhary, Sandeep Kumar
The African Baobab, also known as Kalpvriksha (Adansonia digitata L.), is a significant multi-purpose tree that has gained popularity among people, primarily in African countries, for its use as food, fodder, clothing, and ropes. There is one of the obstacle arises in growing this plant by farmers in India is the lack of planting materials. The young plants of A. digitata L. have to be imported form selected nurseries. Therefore, micropropagation can be used as a substitute method to create offspring that are genetically similar to the parent plant. In order to micropropagate Kalpvriksh (A. digitata L.), this experiment is being conducted utilizing shoot tip explants. For shoot proliferation, these explants are grown in MS medium containing various concentrations of BAP (6- Benzylamino purine): 0.5, 1.0, 1.5, 2.5, 5.0 mg/L. Shoots are rooted in a medium containing at the same concentration of NAA (α-Napthalene Acetic Acid) 0.5 mg/L alone or with combination. Complete Randomized Design (CRD) is used to conduct each experiment. ANOVA and the Duncan New Multiple Range test are used to examine the data. According to the technique for micropropagation of A. digitata L. The concentration of BAP and NAA was found to be 1.5 mg/L and 0.5 mg/L respectively which showed better enhancement for shoot regeneration and rooting for micropropagation of Kalpvriksha (Adansonia digitata L.) tree.
非洲猴面包树，也称为Kalpvriksha（Adansonia digitata L.），是一种重要的多用途树木，因其用作食物、饲料、衣服和绳索而在人们中广受欢迎，主要在非洲国家。印度农民种植这种植物的障碍之一是缺乏种植材料。洋地黄的幼苗必须从选定的苗圃进口。因此，微繁殖可以作为一种替代方法来创造与亲本植物基因相似的后代。本试验是利用茎尖外植体对洋地黄进行微繁殖。对于芽增殖，这些外植体在含有不同浓度BAP（6-苄基氨基嘌呤）的MS培养基中生长：0.5、1.0、1.5、2.5、5.0mg/L。芽在含有相同浓度的NAA（α-萘乙酸）0.5 mg/L单独或组合的培养基中生根。每个实验采用完全随机设计（CRD）。ANOVA和Duncan New Multiple Range检验用于检验数据。根据洋地黄的微繁殖技术，发现BAP和NAA的浓度分别为1.5mg/L和0.5mg/L，对洋地黄的芽再生和微繁殖生根有较好的促进作用。
{"title":"Effect of BAP (6-Benzylaminopurine) and NAA (α-Napthalene Acetic Acid) treatment on Micropropagation of Adansonia digitata L.","authors":"M. Rehman, M. Chaudhary, Sandeep Kumar","doi":"10.56557/pcbmb/2023/v24i3-48292","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48292","url":null,"abstract":"The African Baobab, also known as Kalpvriksha (Adansonia digitata L.), is a significant multi-purpose tree that has gained popularity among people, primarily in African countries, for its use as food, fodder, clothing, and ropes. There is one of the obstacle arises in growing this plant by farmers in India is the lack of planting materials. The young plants of A. digitata L. have to be imported form selected nurseries. Therefore, micropropagation can be used as a substitute method to create offspring that are genetically similar to the parent plant. In order to micropropagate Kalpvriksh (A. digitata L.), this experiment is being conducted utilizing shoot tip explants. For shoot proliferation, these explants are grown in MS medium containing various concentrations of BAP (6- Benzylamino purine): 0.5, 1.0, 1.5, 2.5, 5.0 mg/L. Shoots are rooted in a medium containing at the same concentration of NAA (α-Napthalene Acetic Acid) 0.5 mg/L alone or with combination. Complete Randomized Design (CRD) is used to conduct each experiment. ANOVA and the Duncan New Multiple Range test are used to examine the data. According to the technique for micropropagation of A. digitata L. The concentration of BAP and NAA was found to be 1.5 mg/L and 0.5 mg/L respectively which showed better enhancement for shoot regeneration and rooting for micropropagation of Kalpvriksha (Adansonia digitata L.) tree. ","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42412687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-29DOI: 10.56557/pcbmb/2023/v24i3-48290
S. Selvakumar, E. Abirami, K. K. Prapanjan
Nanoparticles have played a very important role in modern research. This technology involves the synthesis of nanoparticles with controllable size, shape, and material dispersion at nanometer-scale lengths. In the current work, copper nanoparticles have been synthesized by simple green technology using Persea americana leaf extract. The formation of Cu NPs was monitored by recording UV-Vis absorption spectra showing surface plasmon resonance at 320 nm. The green synthetic copper nanoparticles will be further characterized by FT-IR, FESEM, EDS and XRD. FT-IR identified the presence of active groups and phenolic groups. The crystal morphology and size of the nanoparticles will be determined by FESEM and X-ray diffraction studies. It was found that the average particle size of the copper nanoparticles was in the range of 71 nm. These biosynthetic copper nanoparticles were tested for the photocatalytic dye degradation of commercially important textile dyes such as methylene blue The antioxidant activity of green synthesized nanoparticles from the fruit extract was analysed by DPPH-free radical scavenging assay method. The results show that green synthetic nanoparticles have strong dye degradation potential.
{"title":"Green Synthesis, Characterization, Antioxidant and Photocatalytic Dye Degradation Effects of Copper (Cu) Nanoparticles from the Aqueous Extract of Persea americana","authors":"S. Selvakumar, E. Abirami, K. K. Prapanjan","doi":"10.56557/pcbmb/2023/v24i3-48290","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48290","url":null,"abstract":"Nanoparticles have played a very important role in modern research. This technology involves the synthesis of nanoparticles with controllable size, shape, and material dispersion at nanometer-scale lengths. In the current work, copper nanoparticles have been synthesized by simple green technology using Persea americana leaf extract. The formation of Cu NPs was monitored by recording UV-Vis absorption spectra showing surface plasmon resonance at 320 nm. The green synthetic copper nanoparticles will be further characterized by FT-IR, FESEM, EDS and XRD. FT-IR identified the presence of active groups and phenolic groups. The crystal morphology and size of the nanoparticles will be determined by FESEM and X-ray diffraction studies. It was found that the average particle size of the copper nanoparticles was in the range of 71 nm. These biosynthetic copper nanoparticles were tested for the photocatalytic dye degradation of commercially important textile dyes such as methylene blue The antioxidant activity of green synthesized nanoparticles from the fruit extract was analysed by DPPH-free radical scavenging assay method. The results show that green synthetic nanoparticles have strong dye degradation potential.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42561538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-29DOI: 10.56557/pcbmb/2023/v24i3-48289
S. Selvakumar, D. Sajusha, P. Divyadharshini, A. Joe
Introduction: Cancer is a major problem around the world. Now a day’s cervical cancer becomes the most common cause of cancer in women. The early identification and affordable drugs will help to eliminate the seriousness of cancer. Methods: The in vitro cytotoxicity analysis of chloroform extract of Ctenolepsis cerasiformis was undertaken by the method of MTT assay and the apoptotic activity of the chloroform extract of Ctenolepsis cerasiformis was performed by the flow cytometry analysis. Results: The chloroform extract of Ctenolepsis cerasiformis shown important antiproliferative action on human cervical cancer cells as compared to the normal Vero cell lines. Discussion: There is a need to find out the less toxic or no toxic novel anti-proliferative agent from the medicinal plant sources is paramount importance. Conclusion: Hence, the in vitro cytotoxicity and the apoptotic effects of the chloroform extract of Ctenolepsis cerasiformis on human cervical cancer cell lines such as HeLa cell lines were undertaken.
{"title":"Cytotoxicity and Apoptotic Inducing Efficacy of Ctenolepsis cerasiformis Extract on Hela Cervical Cancer Cells","authors":"S. Selvakumar, D. Sajusha, P. Divyadharshini, A. Joe","doi":"10.56557/pcbmb/2023/v24i3-48289","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48289","url":null,"abstract":"Introduction: Cancer is a major problem around the world. Now a day’s cervical cancer becomes the most common cause of cancer in women. The early identification and affordable drugs will help to eliminate the seriousness of cancer. \u0000Methods: The in vitro cytotoxicity analysis of chloroform extract of Ctenolepsis cerasiformis was undertaken by the method of MTT assay and the apoptotic activity of the chloroform extract of Ctenolepsis cerasiformis was performed by the flow cytometry analysis. \u0000Results: The chloroform extract of Ctenolepsis cerasiformis shown important antiproliferative action on human cervical cancer cells as compared to the normal Vero cell lines. \u0000Discussion: There is a need to find out the less toxic or no toxic novel anti-proliferative agent from the medicinal plant sources is paramount importance. \u0000Conclusion: Hence, the in vitro cytotoxicity and the apoptotic effects of the chloroform extract of Ctenolepsis cerasiformis on human cervical cancer cell lines such as HeLa cell lines were undertaken.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47130874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.56557/pcbmb/2023/v24i3-48245
Aditi Shrivastav, M. Tripathi, S. Tiwari, Niraj Tripathi, P. Tiwari, S. Bimal, Poonam Rajpoot, S. Chauhan
Indian mustard (Brassica juncea var. rugosa) constitutes an authoritative group of mustard crops in India. Evaluation of genetic diversity is a vivacious component of mustard breeding programmes for efficient utilization of plant genetic resources. In present study, 77 microsatellite markers were employed to assess the genetic diversity of 75 Indian mustard genotypes. Results revealed positive amplification for all SSRs, with 21 SSRs exhibiting polymorphic amplicons. A total of 99 alleles, ranging from 3 to 5 with an average of 4.71 alleles per SSR marker were obtained. The major allele frequency varied between 0.26 (cnu/m616) and 0.56 (ENA28F) with an average value of 0.36. The average polymorphic information content (PIC) value was 0.67 and ranged between 0.43 (ENA28F) and 0.76 (gi258660710gbGT071338.1). Mean value of 0.72 was detected for each pair of SSR primers, with the gene diversity per locus ranging between 0.53 (ENA28F) and 0.79 (gi258660710gbGT071338.1). The dendrogram grouped the 75 genotypes into three main clusters or subpopulations based on Unweighted Neighbour-Joining technique. The study revealed better understanding of the genetic diversity among different Indian mustard genotypes using genomic-SSR markers that could be exploited for the genetic improvement of the crop.
{"title":"Evaluation of Genetic Diversity in Indian Mustard (Brassica juncea var. rugosa) Employing SSR Molecular Markers","authors":"Aditi Shrivastav, M. Tripathi, S. Tiwari, Niraj Tripathi, P. Tiwari, S. Bimal, Poonam Rajpoot, S. Chauhan","doi":"10.56557/pcbmb/2023/v24i3-48245","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48245","url":null,"abstract":"Indian mustard (Brassica juncea var. rugosa) constitutes an authoritative group of mustard crops in India. Evaluation of genetic diversity is a vivacious component of mustard breeding programmes for efficient utilization of plant genetic resources. In present study, 77 microsatellite markers were employed to assess the genetic diversity of 75 Indian mustard genotypes. Results revealed positive amplification for all SSRs, with 21 SSRs exhibiting polymorphic amplicons. A total of 99 alleles, ranging from 3 to 5 with an average of 4.71 alleles per SSR marker were obtained. The major allele frequency varied between 0.26 (cnu/m616) and 0.56 (ENA28F) with an average value of 0.36. The average polymorphic information content (PIC) value was 0.67 and ranged between 0.43 (ENA28F) and 0.76 (gi258660710gbGT071338.1). Mean value of 0.72 was detected for each pair of SSR primers, with the gene diversity per locus ranging between 0.53 (ENA28F) and 0.79 (gi258660710gbGT071338.1). The dendrogram grouped the 75 genotypes into three main clusters or subpopulations based on Unweighted Neighbour-Joining technique. The study revealed better understanding of the genetic diversity among different Indian mustard genotypes using genomic-SSR markers that could be exploited for the genetic improvement of the crop.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44335682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-11DOI: 10.56557/pcbmb/2023/v24i3-48239
Shri R. P. Rajput, Sudhanshu Jain, S. Tiwari, Akash Barela, S. Chauhan, P. Tiwari, N. Gupta, R. S. Sikarwar, Niraj Tripathi, M. Tripathi
Chickpea (Cicer arietinum L.) is one of the most important legumes in the developed as well as developing countries of the world due to its amazing source of dietary proteins. The present experiment was conducted at Research Farm, RVSKVV, College of Agriculture; Gwalior (M.P.). Forty chickpea genotypes were evaluated during Rabi 2020-21 in Randomized block design with two replications. The samples were analyzed for the analysis of peroxidase, Superoxide Dismutase and some of the key biomolecules like total sugars and proline content. The Total sugar ranged from 16.8 mgg-1 (RVSVT PS-2019- 201) to 24.9 mgg-1 (RVSVT PS-2019- 214), proline varied from 1.49 μmolg-1 (RVSVTK-2019- 104) to 2.91 μmolg-1 (RVSVT PS-2019- 213), superoxide dismutase ranged from 8.2 nmolg-1 (RVSVT PS-2019- 211) to 20.1nmolg-1 (RVSVT PS-2019- 205) and lipid peroxide varied from 1.04 nmolg-1 (RVSVTK-2019-101) to 1.92 nmolg-1 (RVSVTD-2019- 4, RVSVTD-2019- 10). Findings of the present investigation may help to choose better genotypes with useful biochemical contents for future crop improvement.
{"title":"Biochemical Characterization of Chickpea (Cicer arietinum L.) Genotypes","authors":"Shri R. P. Rajput, Sudhanshu Jain, S. Tiwari, Akash Barela, S. Chauhan, P. Tiwari, N. Gupta, R. S. Sikarwar, Niraj Tripathi, M. Tripathi","doi":"10.56557/pcbmb/2023/v24i3-48239","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48239","url":null,"abstract":"Chickpea (Cicer arietinum L.) is one of the most important legumes in the developed as well as developing countries of the world due to its amazing source of dietary proteins. The present experiment was conducted at Research Farm, RVSKVV, College of Agriculture; Gwalior (M.P.). Forty chickpea genotypes were evaluated during Rabi 2020-21 in Randomized block design with two replications. The samples were analyzed for the analysis of peroxidase, Superoxide Dismutase and some of the key biomolecules like total sugars and proline content. The Total sugar ranged from 16.8 mgg-1 (RVSVT PS-2019- 201) to 24.9 mgg-1 (RVSVT PS-2019- 214), proline varied from 1.49 μmolg-1 (RVSVTK-2019- 104) to 2.91 μmolg-1 (RVSVT PS-2019- 213), superoxide dismutase ranged from 8.2 nmolg-1 (RVSVT PS-2019- 211) to 20.1nmolg-1 (RVSVT PS-2019- 205) and lipid peroxide varied from 1.04 nmolg-1 (RVSVTK-2019-101) to 1.92 nmolg-1 (RVSVTD-2019- 4, RVSVTD-2019- 10). Findings of the present investigation may help to choose better genotypes with useful biochemical contents for future crop improvement.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42405032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-24DOI: 10.56557/pcbmb/2023/v24i1-28217
C. Goel, R. Dutta
The species of Adansonia Digitata L. (Baobab) is an important multipurpose tree found to possess an enormous range of medicinal properties and can be used for the treatment of various diseases. Powdered leaves and stem of Adansonia digitata were extracted with 100% ethanol and hydro ethanol using the Soxhlet method of extraction. This research was conducted to determine the antimicrobial efficacy of Adansonia digitata stem and leaf extracts. The antimicrobial activity of ethanolic and hydroethanolic leaf, stem extracts of Adansonia digitata has been analyzed. The activity of the extracts of different plant parts against both bacteria and fungi strains were screened through disk diffusion assay along with the crude extract was carried out as control experiments. The antimicrobial activity of the leaf and stem extract of the plants showed that the plant extract used was effective against the different isolates tested. According to our observation, ethanolic extract of leave and stems showed higher antimicrobial activity.
{"title":"Antimicrobial Activity of Leaf and Stem Extracts of Adansonia digitata","authors":"C. Goel, R. Dutta","doi":"10.56557/pcbmb/2023/v24i1-28217","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28217","url":null,"abstract":"The species of Adansonia Digitata L. (Baobab) is an important multipurpose tree found to possess an enormous range of medicinal properties and can be used for the treatment of various diseases. Powdered leaves and stem of Adansonia digitata were extracted with 100% ethanol and hydro ethanol using the Soxhlet method of extraction. This research was conducted to determine the antimicrobial efficacy of Adansonia digitata stem and leaf extracts. The antimicrobial activity of ethanolic and hydroethanolic leaf, stem extracts of Adansonia digitata has been analyzed. The activity of the extracts of different plant parts against both bacteria and fungi strains were screened through disk diffusion assay along with the crude extract was carried out as control experiments. The antimicrobial activity of the leaf and stem extract of the plants showed that the plant extract used was effective against the different isolates tested. According to our observation, ethanolic extract of leave and stems showed higher antimicrobial activity.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42525064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-20DOI: 10.56557/pcbmb/2023/v24i1-28214
Shivangi Tare, S. Tiwari, M. Rathore, M. Yasin, Niraj Tripathi, P. Tiwari, M. Tripathi
The primary goal of every plant breeder is to identify gene alleles and then use them in crop improvement programmes. If there is no population variation, there cannot be a breeding programme. Breeders need a lot of labour and money to screen germplasm for a desired gene. Additionally, these screenings are compliant with environmental effects. The method of "allele mining" is employed to identify suitable alleles of a candidate gene affecting important agronomic properties or naturally occurring allelic variations. TILLING and Eco-TILLING are the ideal solutions to this issue for allele mining. A technique known as "tilling" uses mutagens to introduce new diversity in a specific allele. Then, different sequencers are used to screen the diversity in a gene to identify different mutations. The best mutant among them can then be directly used in breeding programmes. In a modified version, alleles of a gene that are present in the population are identified by screening natural populations. Eco-TILLING is the name of this fresh iteration of the technology. The generation of novel haplotypes, the use of molecular markers to characterize genetic diversity and the syntenic links across crop genotypes, as well as marker-assisted selection are just a few of the many applications of allele mining in agriculture and crop improvement. Many genes may be found and used in the breeding of many crop species using these reverse genetics techniques.Allele mining
{"title":"An Overview of Allele Mining for Crop Improvement: TILLING and Eco-TILLING","authors":"Shivangi Tare, S. Tiwari, M. Rathore, M. Yasin, Niraj Tripathi, P. Tiwari, M. Tripathi","doi":"10.56557/pcbmb/2023/v24i1-28214","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28214","url":null,"abstract":"The primary goal of every plant breeder is to identify gene alleles and then use them in crop improvement programmes. If there is no population variation, there cannot be a breeding programme. Breeders need a lot of labour and money to screen germplasm for a desired gene. Additionally, these screenings are compliant with environmental effects. The method of \"allele mining\" is employed to identify suitable alleles of a candidate gene affecting important agronomic properties or naturally occurring allelic variations. TILLING and Eco-TILLING are the ideal solutions to this issue for allele mining. A technique known as \"tilling\" uses mutagens to introduce new diversity in a specific allele. Then, different sequencers are used to screen the diversity in a gene to identify different mutations. The best mutant among them can then be directly used in breeding programmes. In a modified version, alleles of a gene that are present in the population are identified by screening natural populations. Eco-TILLING is the name of this fresh iteration of the technology. The generation of novel haplotypes, the use of molecular markers to characterize genetic diversity and the syntenic links across crop genotypes, as well as marker-assisted selection are just a few of the many applications of allele mining in agriculture and crop improvement. Many genes may be found and used in the breeding of many crop species using these reverse genetics techniques.Allele mining","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45392553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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