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Multispectral autoflourescence detection of skin neoplasia using steady-state techniques 使用稳态技术的多光谱自体荧光检测皮肤肿瘤
E. Borisova, A. Gisbrecht, Tsanislava Genova-Hristova, P. Troyanova, E. Pavlova, N. Penkov, I. Bratchenko, V. Zakharov, Ilze Lihachova, I. Kuzmina, J. Spigulis
In the current study were used excitation-emission matrices (EEMs) and synchronous fluorescence spectroscopy (SFS) steady-state techniques in a broad spectral regions (excitation at 220-500 nm and emission at 280-850 nm) to achieve the whole set of endogenous fluorophores, existed in normal and neoplastic cutaneous tissues. Several types of benign, dysplastic and malignant types of skin lesions were investigated ex vivo using both EEM and SFS modalities, namely the basal cell papilloma and carcinoma, pigmented nevi, dysplastic nevi, squamous cell carcinoma and malignant melanoma. Histological analysis was used as a “gold standard” for evaluation of clinical diagnosis of the lesions investigated. Comparison with the normal skin tissue spectral data was made, based on the signals detected from the safety margins areas of the surgically excised tumours. EEM and SFS data reveal statistically significant differences between variety of benign, dysplastic and malignant lesions, which could be used as fingerprints, applicable for differentiation algorithms. In a few of malignancies endogenous porphyrins signals were even observed, but in general the fluorescence signals were addressed to the coenzymes, such as NADH, flavins; structural proteins, such as collagen, elastin and their cross-links, as well as keratin in the case of basal cell lesions. Pigments, such as hemoglobin and melanin distorted the signal due to intrinsic fluorophores signal reabsorption, what has to be taken into account when the algorithms for discrimination of the pathology types are developed.
本研究利用广谱区(220-500 nm激发,280-850 nm发射)的激发-发射矩阵(EEMs)和同步荧光光谱(SFS)稳态技术,获得了存在于正常和肿瘤皮肤组织中的全套内源性荧光团。在体外使用EEM和SFS方法研究了几种类型的良性、发育不良和恶性皮肤病变,即基底细胞乳头状瘤和癌、色素痣、发育不良痣、鳞状细胞癌和恶性黑色素瘤。组织学分析被用作评估所调查病变临床诊断的“金标准”。根据从手术切除肿瘤的安全边缘区域检测到的信号,与正常皮肤组织光谱数据进行比较。EEM和SFS数据显示各种良性、发育不良和恶性病变之间存在统计学差异,可以作为指纹,适用于鉴别算法。在一些恶性肿瘤中甚至观察到内源性卟啉信号,但一般荧光信号是针对辅酶,如NADH,黄素;结构蛋白,如胶原蛋白,弹性蛋白及其交联,以及角蛋白在基底细胞病变的情况下。色素,如血红蛋白和黑色素,由于固有的荧光团信号重吸收而扭曲了信号,这是在开发病理类型区分算法时必须考虑的问题。
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引用次数: 6
Light irradiation effect on the gas sensing properties of the ZnO nanostructures 光辐照对ZnO纳米结构气敏性能的影响
G. Atanasova, T. Dilova, A. Dikovska, G. Avdeev, P. Stefanov, N. Nedyalkov
In this work, we present fabrication of ZnO nanostructures by pulsed laser deposition in air at atmospheric pressure. The use of this technology leads to formation of nanostructures composed by nanoparticles and nanoaggregates. These nanostructures possess a large surface-to-volume ratio, which makes them suitable for gas-sensor application. The samples were exposed to NH3 and the effect was investigated of light irradiation on the gas response and recovery time of the sensor element. It was found that the response of the sensor element increases even by irradiation by sunlight. The gas sensing properties of the ZnO nanostructures were compared when irradiated by light of different wavelengths.
在这项工作中,我们提出了用脉冲激光沉积法制备ZnO纳米结构的方法。这种技术的使用导致形成由纳米颗粒和纳米聚集体组成的纳米结构。这些纳米结构具有较大的表面体积比,这使得它们适合于气体传感器的应用。将样品暴露于NH3中,研究光照对传感器元件气体响应和恢复时间的影响。结果表明,即使在阳光照射下,传感器元件的响应也会增加。比较了ZnO纳米结构在不同波长光照射下的气敏性能。
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引用次数: 0
Luminescence of iridium complexes upon short laser pulses 短激光脉冲下铱配合物的发光
N. Dimitrov, G. Hadjichristov, I. Stefanov
The photo-excited luminescence response of solutions (in organic solvent) of eight selected organometallic coordination complexes of Iridium Ir(III) ion is studied upon irradiation with high-intensity short-pulse (nanosecond) ultraviolet laser irradiation, as well as by ultra-high-intensity ultrashort (femtosecond) laser pulses in the visible (violet). The Ir-complexes are cyclometalated and contain bis-phenylbenzothiazole as a main ligands and auxiliary β-diketone ligands, and they are intended to be used as luminescent materials and sensitive nanoprobes, e.g., in organic optoelectronics, sensorics, mechatronics and laser fluorescent microscopy. The results obtained by laser spectroscopy of dilute (10–5 M) solutions of the examined Ir-complexes show that they are efficient triplet emitters whose photo-excited emission is proper for fluorometric, optoelectronic and photonic applications.
采用高强度短脉冲(纳秒)紫外激光和超高强度超短(飞秒)激光在可见光(紫外光)照射下,研究了选定的8种铱Ir(III)离子有机金属配合物溶液(在有机溶剂中)的光激发发光响应。ir -配合物是环金属化的,含有双苯基苯并噻唑作为主配体和辅助配体β-二酮,可作为发光材料和敏感的纳米探针,如有机光电子学、传感学、机电一体化和激光荧光显微镜。对所研究的ir配合物的稀释(10-5 M)溶液的激光光谱结果表明,它们是有效的三重态发射体,其光激发发射适合于荧光学、光电学和光子学应用。
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引用次数: 0
Six months follow-up with pulse oximetry and electric pulp test of teeth with trauma 随访6个月,对外伤牙进行脉搏血氧测定和电髓试验
D. Kosturkov, T. Uzunov
Early diagnosis of pulp condition in cases of teeth with trauma is very important not only to preserve the vitality of the pulp but also to prevent both early and late traumatic complications. We investigated 120 frontal teeth with traumas. The teeth were tested on 1-st day, 15-th day, 30-th day, 3-rd month, 6-th month by pulse oximetry (PO) and electric pulp test (EPT). The results we received from pulse oximetry show that the saturation levels immediately after the trauma are very high, reaching 90%. Over the time, there has been a tendency for reduction of saturation, reaching a level of 82% by the 6th month, which is within the limit of the norm. The differences between the obtained saturation levels are statistically significant up to the 30th day of the trauma (p <0.05). The EPT results showed that the highest values were immediately after the trauma - 53 μA. The average values obtained for all periods up to and including 3-rd month are higher than the established norms. They differ statistically between each other and from the values, measured at 6-th month, which are average 8 μA. Comparing the circulatory response and the innervation of the traumatized teeth, we could conclude that the blood circulation recovers more quickly within one month, whereas the nerve conduction is restored until the 6-th month. Thus, the implementation of pulse oximetry in dentistry is of great benefit for the total pulp diagnosis – not only of the nerve, but also of the blood circulation.
牙髓损伤的早期诊断不仅对保持牙髓的活力,而且对预防早期和晚期的创伤并发症具有重要意义。我们调查了120颗有外伤的门牙。分别于第1天、第15天、第30天、第3个月、第6个月进行脉搏血氧仪(PO)和电牙髓试验(EPT)。我们从脉搏血氧计中得到的结果显示,创伤后的饱和度非常高,达到90%。随着时间的推移,饱和度有降低的趋势,到第6个月达到82%的水平,这在正常范围内。在创伤后30天,两组间的饱和度差异有统计学意义(p <0.05)。EPT结果显示,创伤后立即达到最高值- 53 μA。截至并包括第3个月的所有期间的平均值高于既定的标准。与第6个月测得的平均值(8 μA)有统计学差异。对比损伤牙的循环反应和神经支配,我们可以得出结论:损伤牙的血液循环在1个月内恢复得更快,而神经传导则在6个月后恢复。因此,在牙科中实施脉搏血氧仪对全髓诊断有很大的好处-不仅是神经,而且是血液循环。
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引用次数: 0
Lidar and contact investigations of aerosol characteristics near high traffic urban sites 高交通流量城市站点附近气溶胶特征的激光雷达和接触调查
R. Angelova, L. Slavov, G. Kolarov, I. Grigorov, C. Ghelev, L. Gurdev, I. Nedkov, D. Stoyanov
In this work some features are revealed, simultaneously by LIDAR and contact facilities, of the aerosol pollutions near high-traffic sites in Sofia City. Such features of interest are the extinction coefficient of the aerosol ensembles and their mass concentration, as well as the shapes and sizes and the structure of the aerosol particles and their chemical composition and biological content. They outline possible negative effects of the aerosol pollutants on the human health. The experiments are conducted in the spring and summer, in different sites of interest in Sofia City. The results about the aerosol mass concentration-to-extinction calibration of the LIDAR constant, obtained in the spring and in the late spring and the summer, are consistent with each other and with the combined spring-and-summer result. Using electron microscopy, various aerosol samples are visualized resembling mineral dust particles, pollen, soot, and even microorganisms. The results from the analysis of the images obtained would serve as guidelines of further chemical and microbiological studies.
在这项工作中,通过激光雷达和接触设备同时揭示了索非亚市高流量站点附近气溶胶污染的一些特征。这些令人感兴趣的特征是气溶胶群的消光系数及其质量浓度,以及气溶胶颗粒的形状、大小和结构及其化学成分和生物含量。他们概述了气溶胶污染物对人类健康可能产生的负面影响。实验于春季和夏季在索非亚市不同的景点进行。春季和春末夏季的气溶胶质量浓度-消光校正结果与春季和夏季联合校正结果基本一致。使用电子显微镜,各种气溶胶样品被可视化,类似矿物粉尘颗粒,花粉,煤烟,甚至微生物。所获得的图像分析结果将作为进一步化学和微生物研究的指导方针。
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引用次数: 0
Vortex solutions of vector nonlinear amplitude equations in optics 光学中矢量非线性振幅方程的涡旋解
I. Bozhikoliev, K. Kovachev, A. Dakova, V. Slavchev, D. Dakova, L. Kovachev
Different kind of vortex structures of laser beam can be created by optical holograms and different optical masks. In the theory these vortices are solutions of the 2D scalar Leontovich equations. These solutions admit amplitude and phase singularities. The main tack of this work is to investigate the possibility of formation of vortex structures for narrow-band optical pulses, propagating in Kerr-type media. The evolution of such type of laser pulses is governed by nonlinear vector system of amplitude equations in second approximation of the linear dispersion. We found new class of analytical solutions with vortex structures. The nonlinear dispersion relations obtained by these vortex solutions show that their stability is due not only to balance between diffraction and nonlinearity, but also to a balance between non-linearity and angular distribution.
利用光学全息图和不同的光学掩模可以产生不同类型的激光束漩涡结构。在理论上,这些涡旋是二维标量列昂托维奇方程的解。这些解承认振幅和相位的奇异性。这项工作的主要方向是研究在克尔型介质中传播的窄带光脉冲形成涡旋结构的可能性。这种类型的激光脉冲的演化是由线性色散二阶近似的非线性振幅方程矢量系统控制的。我们发现了一类新的涡结构解析解。这些涡旋解的非线性色散关系表明,它们的稳定性不仅是由于衍射和非线性之间的平衡,而且是由于非线性和角分布之间的平衡。
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引用次数: 1
Interference wedged structures as light beam splitting elements 作为分束元件的干涉楔形结构
M. Deneva, M. Nenchev, E. Stoykova
Based on our experience in the field of the interferential wedge, we present a new competitive application of such optical elements and structures built from them with theoretical description and experimental verification. We have demonstrated that a conventional interferential wedge or a structure of two wedges in compact layered implementation can be used as an attractive simple light power splitting element for a spatially and spectrally narrow light beam (basically a laser beam). The element can provide: 1) precisely and variably controlled ratio of the reflected and transmitted power by simple sliding in its plane of the list-like wedged structure with an apex angle of ~10-5 rad; 2) division practically without energy losses; 3) power ratio control without causing change of propagation direction of the reflected and the transmitted beams that is of essential interest for applications in optical schemes with complex geometry of the beams propagation; no optical properties variation of the reflective layers (mirrors) during the sliding; 4) working at beam power densities of MW/cm2 - GW /cm2 when the optical element is built from optical materials (layers) with high light damage resistivity.
根据我们在干涉楔领域的经验,我们提出了这种光学元件和由它们构建的结构的新的竞争性应用,并进行了理论描述和实验验证。我们已经证明了传统的干涉楔或紧凑分层实现的两个楔结构可以用作空间和光谱狭窄光束(基本上是激光束)的有吸引力的简单光功率分裂元件。该元件可以提供:1)通过在其平面上以~10-5 rad的顶点角进行单楔状结构的简单滑动,精确可变地控制反射功率和透射功率的比值;2)分区几乎无能量损失;3)在不改变反射和发射光束传播方向的情况下控制功率比,这对于光束传播几何形状复杂的光学方案的应用至关重要;滑动过程中反射层(反射镜)光学特性无变化;4)当光学元件由具有高光损伤电阻率的光学材料(层)制成时,工作在光束功率密度为MW/cm2 - GW /cm2的情况下。
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引用次数: 2
Vortex interactions revisited: Formation of stable elementary cells for creation of rigid vortex lattices 涡旋相互作用重访:形成刚性涡旋晶格的稳定基本单元
L. Stoyanov, N. Gorunski, M. Zhekova, I. Stefanov, A. Dreischuh
Optical vortices (OVs) are the only known truly two-dimensional phase dislocations. Because of their spiral phase fronts, the OV interaction results, in the simplest case (when two OVs are presented), in vortex mutual attraction/repulsion or in OV pair rotation. In this work we provide experimental evidences that a stable elementary cell forming the base for a large optical vortex lattice can be created by situating equally and singly charged OVs in the apices of a triangle and square and by nesting an additional control OV with an opposite unit charge in the center of the structure. Experimental data for the rotation of these triangular and quadratic elementary cells vs. OV-to-OV separation as well as the rotation of the same structures vs. propagation distance are presented. Generation and stable propagation of large rigid square-shaped and hexagonal OV lattices is demonstrated.
光涡旋(OVs)是唯一已知的真正的二维相位错。由于它们的螺旋相位前沿,在最简单的情况下(当存在两个OV时),OV相互作用导致涡旋相互吸引/排斥或OV对旋转。在这项工作中,我们提供了实验证据,证明通过在三角形和正方形的顶端放置等电荷和单电荷的OV,并在结构的中心嵌套一个具有相反单位电荷的额外控制OV,可以创建一个稳定的基本单元,形成大型光学涡旋晶格的基础。给出了这些三角形和二次基元的旋转相对于OV-to-OV分离的实验数据,以及相同结构的旋转相对于传播距离的实验数据。证明了大型刚性方形和六边形OV晶格的生成和稳定传播。
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引用次数: 3
Comparative study of the characteristics of red Bulgarian and French wines using applied photonics methods 应用光子学方法对保加利亚红酒和法国红酒特性的比较研究
Nadia Hristova Aqakumova, V. Hadjimitova, K. Nikolova, S. Minkova, T. Evtimov, R. Gabrova, V. Vladev
The fluorescence spectra of Bulgarian and French wines of the same price class were obtained at excitation wavelengths of 265 nm, 275 nm and 295 nm. The excitation-emission matrices are obtained and compared. A correlation between the emission maxima and the anthocyanins or the antioxidant activity expressed in Trollox units is obtained.
在265 nm、275 nm和295 nm激发波长下,获得了保加利亚和法国同价位葡萄酒的荧光光谱。得到了激发-发射矩阵,并进行了比较。得到了辐射最大值与花青素或特罗洛克斯单位表达的抗氧化活性之间的相关性。
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引用次数: 0
Saharan dust mixed with marine aerosols: lidar measurements and characterization 撒哈拉沙尘与海洋气溶胶混合:激光雷达测量和表征
Z. Peshev, A. Deleva, T. Dreischuh
Mineral aerosols from Sahara desert are frequently transported to Europe by regional or trans-continental air circulations. During such a long-range transport, Saharan dust is naturally mixed with other aerosols. The pathway of desert aerosols frequently passes over the Mediterranean Sea or the Atlantic Ocean. Under certain conditions, dust particles can blend with marine aerosols to form a mixture with specific volume/mass concentration and particle size distribution. These parameters are determinative for the climatologic, ecologic and medical impacts of the aerosols. Therefore, studies of desert-marine aerosols’ interacting and mixing are of considerable scientific and practical importance. In this work, we report results of lidar measurements and analysis of Saharan dust layers observed over Sofia, Bulgaria, possibly containing considerable amounts of marine aerosols captured and mixed with the dust particulate matter during the passage of air masses close to the surface of the Mediterranean Sea and/or Atlantic Ocean. The aerosol layer’s origin is proven by using air mass transport modeling and forecasting data. Measurements are carried out at two wavelengths (1064 nm and 532 nm) by means of the two aerosol channels of a lidar based on a frequency-doubled Nd:YAG laser. Topological, dynamical, optical, and microphysical properties of the dust/aerosol layers are studied and analyzed. Timeaveraged vertical profiles of the atmospheric backscattering coefficient at 1064 nm and 532 nm are presented. By using backscatter-related Ångström exponents, microphysical properties of aerosol particles are characterized qualitatively. Color map diagrams illustrate the temporal evolution of the aerosol density height distribution. Conclusions concerning the effects of dust mixing with marine aerosols are drawn.
来自撒哈拉沙漠的矿物气溶胶经常通过区域或跨大陆的空气环流输送到欧洲。在这样的长途运输中,撒哈拉沙尘自然地与其他气溶胶混合在一起。沙漠气溶胶的路径经常经过地中海或大西洋。在一定条件下,粉尘颗粒可与海洋气溶胶混合,形成具有比容质量浓度和粒径分布的混合物。这些参数对气溶胶的气候、生态和医学影响具有决定性作用。因此,研究沙漠-海洋气溶胶的相互作用和混合具有重要的科学意义和实际意义。在这项工作中,我们报告了在保加利亚索非亚观测到的撒哈拉沙尘层的激光雷达测量和分析结果,可能含有大量的海洋气溶胶,并在靠近地中海和/或大西洋表面的气团通过时与沙尘颗粒物质混合。利用气团输运模式和预报资料证实了气雾层的起源。利用双频Nd:YAG激光雷达的两个气溶胶通道,在两个波长(1064 nm和532 nm)下进行了测量。研究和分析了尘埃/气溶胶层的拓扑、动力学、光学和微物理特性。给出了大气后向散射系数在1064 nm和532 nm处的时间平均垂直剖面。利用与后向散射相关的Ångström指数,定性地表征了气溶胶粒子的微物理性质。彩色地图说明了气溶胶密度高度分布的时间演变。得出了粉尘与海洋气溶胶混合影响的结论。
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引用次数: 1
期刊
International School on Quantum Electronics: Laser Physics and Applications
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