Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.04317
Gülhan Işik, A. Tezcaner, N. Hasirci, A. Kiziltay
Objective: The aim of this study was to find the efficient medium to bind an anticancer drug, Doxorubicin (DOX), to a synthesized polymer, methoxy poly(ethylene glycol)-block-polycaprolactone (mPEG-b-PCL) via pH sensitive hydrazone bonds and to determine the amount of conjugated drug. Methods: DOX conjugation was carried out in two different media [dimethyl sulfoxide (DMSO) and methanol-trifluoroacetic acid (MeOH-TFA)]. The amount of conjugated drug was determined with two different methods. One method was applied the dissolution of the conjugate in chloroform: methanol (Ch: MeOH, 1: 1 v/v) solution without considering pH responsiveness, and the other method was after breaking pH sensitive hydrazone bonds in acidic medium [using three different media as 0.1 M hydrochloric acid (HCl), concentrated HCl (12 M HCl) and concentrated sulfuric acid (18.3 M H2SO4)]. Results: The highest conjugation efficiency was obtained when the conjugation was achieved in MeOH-TFA solution, and for the polymer-drug conjugates after the treatment with concentrated sulfuric acid. recognized by immune system rapidly (12). On the other hand, PEG is hydrophilic and can escape from the immune system. Scientists prepared or used copolymers or tri-block-polymers of PEG and PCL and prepared either nanoparticles or micelles as drug delivery vehicles and evaluated in vitro and in vivo properties (13). Gong et al studied affinity of PEG-PCL-PEG micelles (14). Hu et al prepared micelles and polymersomes from PCL-PEG-PCL polymers to be used as drug carriers (15). Cytotoxic properties of Doxorubicin loaded PEG-PCL micelles prepared with functionalized PEGs were evaluated by Chen et al. and promising results were reported (16). An accurate determination of drug conjugation is important since higher dose given to a patient may create some unwanted symptoms like cardiomyopathy. In this study, methoxy konjugasyonu Conclusion: It was concluded that, MeOH-TFA method was a good method for conjugation of DOX to M) method was better other present in literature for determination of the amount of DOX linked to the polymer via
目的:寻找抗肿瘤药物多柔比星(DOX)与甲氧基聚乙二醇嵌段聚己内酯(mPEG-b-PCL)通过pH敏感腙键结合的有效介质，并测定结合药物的量。方法:在两种不同的介质[二甲亚砜(DMSO)和甲醇-三氟乙酸(MeOH-TFA)]中进行DOX偶联。采用两种不同的方法测定结合药物的用量。一种方法是在氯仿:甲醇(Ch: MeOH, 1:1 v/v)溶液中溶解共轭物，不考虑pH响应性;另一种方法是在酸性介质中破坏pH敏感的腙键[使用0.1 M盐酸(HCl)、浓盐酸(12 M HCl)和浓硫酸(18.3 M H2SO4)三种不同的介质]。结果:在MeOH-TFA溶液中偶联和浓硫酸处理后的聚合物-药物偶联物的偶联效率最高。迅速被免疫系统识别(12)。另一方面，聚乙二醇是亲水的，可以从免疫系统中逃脱。科学家们制备或使用PEG和PCL的共聚物或三嵌段聚合物，并制备纳米颗粒或胶束作为药物传递载体，并评估其体外和体内性能(13)。Gong等研究了PEG-PCL-PEG胶束的亲和力(14)。Hu等人用PCL-PEG-PCL聚合物制备胶束和聚合体作为药物载体(15)。Chen等人对功能化聚乙二醇制备的负载多柔比星的PEG-PCL胶束的细胞毒性进行了评估，并报道了有希望的结果(16)。准确测定药物偶联是很重要的，因为给病人高剂量可能会产生一些不想要的症状，如心肌病。结论:本文认为，MeOH-TFA法是测定DOX与M - tfa偶联的较好方法，M - tfa法是测定DOX通过聚合物偶联量的较好方法
{"title":"Determination of doxorubicin amount conjugated to mPEG-b-PCL copolymer via pH sensitive hydrazone bond","authors":"Gülhan Işik, A. Tezcaner, N. Hasirci, A. Kiziltay","doi":"10.5505/turkhijyen.2022.04317","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.04317","url":null,"abstract":"Objective: The aim of this study was to find the efficient medium to bind an anticancer drug, Doxorubicin (DOX), to a synthesized polymer, methoxy poly(ethylene glycol)-block-polycaprolactone (mPEG-b-PCL) via pH sensitive hydrazone bonds and to determine the amount of conjugated drug. Methods: DOX conjugation was carried out in two different media [dimethyl sulfoxide (DMSO) and methanol-trifluoroacetic acid (MeOH-TFA)]. The amount of conjugated drug was determined with two different methods. One method was applied the dissolution of the conjugate in chloroform: methanol (Ch: MeOH, 1: 1 v/v) solution without considering pH responsiveness, and the other method was after breaking pH sensitive hydrazone bonds in acidic medium [using three different media as 0.1 M hydrochloric acid (HCl), concentrated HCl (12 M HCl) and concentrated sulfuric acid (18.3 M H2SO4)]. Results: The highest conjugation efficiency was obtained when the conjugation was achieved in MeOH-TFA solution, and for the polymer-drug conjugates after the treatment with concentrated sulfuric acid. recognized by immune system rapidly (12). On the other hand, PEG is hydrophilic and can escape from the immune system. Scientists prepared or used copolymers or tri-block-polymers of PEG and PCL and prepared either nanoparticles or micelles as drug delivery vehicles and evaluated in vitro and in vivo properties (13). Gong et al studied affinity of PEG-PCL-PEG micelles (14). Hu et al prepared micelles and polymersomes from PCL-PEG-PCL polymers to be used as drug carriers (15). Cytotoxic properties of Doxorubicin loaded PEG-PCL micelles prepared with functionalized PEGs were evaluated by Chen et al. and promising results were reported (16). An accurate determination of drug conjugation is important since higher dose given to a patient may create some unwanted symptoms like cardiomyopathy. In this study, methoxy konjugasyonu Conclusion: It was concluded that, MeOH-TFA method was a good method for conjugation of DOX to M) method was better other present in literature for determination of the amount of DOX linked to the polymer via","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.34445
Mehmet Ali Kanat, D. Altun, Kübra Kiliç, Edibe Nurzen Namlı Bozkurt, Ertuğrul Turan, G. Cengi̇z, İlhan Bozyi̇ği̇t
{"title":"Evaluation of cross-protection of Androctonus crassicauda antivenom against Leiurus abdullahbayrami venom.","authors":"Mehmet Ali Kanat, D. Altun, Kübra Kiliç, Edibe Nurzen Namlı Bozkurt, Ertuğrul Turan, G. Cengi̇z, İlhan Bozyi̇ği̇t","doi":"10.5505/turkhijyen.2022.34445","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.34445","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.34538
E. Günaydın, Özlem Kardoğan, G. Goncagül, Yavuz Çokal, Pınar Mursaloğlu Kaynar
{"title":"Salmonella Typhimurium and Salmonella Hessarek in wild birds","authors":"E. Günaydın, Özlem Kardoğan, G. Goncagül, Yavuz Çokal, Pınar Mursaloğlu Kaynar","doi":"10.5505/turkhijyen.2022.34538","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.34538","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.66742
S. Kemer, Sefa Meti̇n, Gökçe Sural, E. Demirel Yilmaz
{"title":"Autacoids in the inflammation","authors":"S. Kemer, Sefa Meti̇n, Gökçe Sural, E. Demirel Yilmaz","doi":"10.5505/turkhijyen.2022.66742","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.66742","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70676883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.60362
B. Çolak, D. Cansaran Duman, E. Yilmaz
Cancer is a disease that occurs in tissues and organs as a result of uncontrolled cell divisions. There are numerous types of cancer related with different frequencies and mortality rate. Ovarian cancer is the seventh common type of cancer worldwide and is the fifth cause of cancer-related death. Although there are standard treatment methods for cancer types, people give different responses to these treatment methods. In spite of all the treatment strategy developed, ovarian cancer still has a high mortality rate. For this reason, it is very important to better understand the drug resistance mechanisms that prevent the effecttiveness of today’s treatment methods, to develop new treatment methods and the determination of molecular characterization. In recent years, research has focused on methods containing pathway analysis within the scope of developing targeted therapy strategies for ovarian cancer. In this context, the activity status of onco-proteins in pathways such as WNT, Hedgehog and NOTCH signaling pathways were evaluated. In addition, the innovative treatment efficiency of Nanog protein and photodynamic therapy options on ovarian cancer has been detailed in recent years. It will be possible to increase the quality of the healing process of ÖZET Kanser, kontrol edilemeyen hücre bölünmeleri sonucunda doku ve organlarda meydana gelen hasarların bütünüdür. Farklı görülme sıklığı ve ölüm oranları ile ilişkili birçok kanser türü vardır. Yumurtalık kanseri dünya genelinde yaygınlık olarak yedinci sırada gelmekte olup kansere bağlı ölüm nedeni olarak beşinci sırada gelmektedir. Kanser türlerinin kendi içinde standart tedavi yöntemleri olmasına rağmen bireyler bu tedavi yöntemlerine farklı cevaplar vermektedir. Geliştirilen tüm tedavi stratejilerine rağmen yumurtalık kanseri hala yüksek ölüm oranına sahiptir. Bu sebeple günümüzdeki tedavi yöntemlerinin etkinliğini engelleyen ilaca karşı oluşan direnç mekanizmalarını kavramak, yeni tedavi yöntemleri geliştirmek ve moleküler karakterizasyonunu belirlemek oldukça önemlidir. Son yıllarda oldukça gündeme gelen yumurtalık kanserinde hedefe yönelik terapi stratejileri geliştirilmesi kapsamında yolak analizleri üzerine odaklanılmıştır. Bu kapsamda onkoproteinlerin WNT, Hedgehog ve NOTCH sinyal yolağı gibi yolaklardaki etkinlik durumları değerlendirilmiştir. Ayrıca, son yıllarda etkinliği belirlenmiş Nanog proteini ve fotodinamik tedavi seçeneklerinin yumurtalık kanseri üzerindeki yenilikçi tedavi etkinliği detaylandırılmıştır. Yumurtalık kanseri tedavisinde mevcut ve geliştirilmesi Türk Hijyen ve Deneysel Biyoloji Dergisi 1Ankara Üniversitesi Biyoteknoloji Enstitüsü, Ankara Geliş Tarihi / Received : Kabul Tarihi / Accepted : İletişim / Corresponding Author : Demet CANSARAN DUMAN Ankara Üniversitesi, Biyoteknoloji Enstitüsü Merkez Laboratuvarı, Tandoğan / Ankara Türkiye E-posta / E-mail : dcansaran@yahoo.com 24.02.2021 16.04.2021 Çolak B, Cansaran Duman D, Yılmaz E. Yumurtalık kanserinde hedefe yönelik te
癌症是一种由于细胞分裂失控而发生在组织和器官中的疾病。有许多类型的癌症与不同的频率和死亡率相关。卵巢癌是全球第七大常见癌症类型，也是癌症相关死亡的第五大原因。虽然癌症有标准的治疗方法，但人们对这些治疗方法的反应不同。尽管开发了各种治疗策略，但卵巢癌的死亡率仍然很高。因此，更好地了解阻碍当今治疗方法有效性的耐药机制，开发新的治疗方法和确定分子表征是非常重要的。近年来，在卵巢癌靶向治疗策略的制定范围内，研究的重点是包含通路分析的方法。在此背景下，我们评估了肿瘤蛋白在WNT、Hedgehog和NOTCH信号通路中的活性状态。此外，近年来Nanog蛋白和光动力治疗方案对卵巢癌的创新治疗效果也有详细的介绍。这将有可能提高愈合过程的质量ÖZET Kanser，控制edilemeyen h cre bölünmeleri sonucunda doku ve organlarda meydana gelen hasarların b tt nnd r。farklyi görülme sıklığı ve ölüm oranlaryi ilil kili birok kanser türü vardır。Yumurtalık kanseri d nya genelinde yaygınlık olarak yedinci sırada gelmekte olup kansere bağlı ölüm nedeni olarak be inci sırada gelmektedir。Kanser trellerinkendi i ' rindinde标准tedavi yöntemleri olmasına rağmen birelyler但tedavi yöntemlerine farklylcevapplar vermektedir。geli tirilen t m tedavi stratejilerine rağmen yumurtalık kanseri hala y ksek ölüm oranına shiptir。Bu sebeple g nnm zdeki tedavi yöntemlerinin etkinliğini engelleyen ilaca kar olu direnç mekanizmalarını kavramak, yeni tedavi yöntemleri geli tirmek ve molek ler karakterizasyonunu belirlemek olduka önemlidir。Son yıllarda olduk g ndeme gelen yumurtalık kanserinde hedefe yönelik terapi stratejileri geli tirilmesi kapsamında yolak analizleri zerine odaklanılmıştır。但是kapsamda onkoprotein in WNT, Hedgehog基因NOTCH信号yolağı gibi yolaklardaki etkinlik durumlari değerlendirilmiştir。Ayrıca, son yıllarda etkinliği belirlenmiki Nanog proteini ve fotodinamik tedavi seeneklerinin yumurtalık kanseri zerindeki yeniliki tedavi etkinliği detaylandırılmıştır。Yumurtalık kanseri tedavisinde mevcut ve geli tirilmesi t rk Hijyen ve Deneysel Biyoloji Dergisi 1Ankara Üniversitesi Biyoteknoloji Enstitüsü， Ankara geliu Tarihi /收件:Kabul Tarihi /收件:İletişim /通讯作者:Demet CANSARAN DUMAN Ankara Üniversitesi, Biyoteknoloji Enstitüsü Merkez laboratuvaryi, Tandoğan / Ankara trkiye E-posta / E-mail:dcansaran@yahoo.com 24.02.2021 16.04.2021 Çolak B, Cansaran Duman D, Yılmaz E. Yumurtalık kanserinde hedefe yönelik tedavi stratejilerinde molekler mekanizmaların rolü Turk Hij Den Biyol Derg, 2021;DOI: 10.5505/TurkHijyen.2022.60362 . DOI: 10.5505/TurkHijyen.2022.60362
{"title":"The Role of Molecular Mechanisms in Targeted Therapy Strategies in Ovarian Cancer","authors":"B. Çolak, D. Cansaran Duman, E. Yilmaz","doi":"10.5505/turkhijyen.2022.60362","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.60362","url":null,"abstract":"Cancer is a disease that occurs in tissues and organs as a result of uncontrolled cell divisions. There are numerous types of cancer related with different frequencies and mortality rate. Ovarian cancer is the seventh common type of cancer worldwide and is the fifth cause of cancer-related death. Although there are standard treatment methods for cancer types, people give different responses to these treatment methods. In spite of all the treatment strategy developed, ovarian cancer still has a high mortality rate. For this reason, it is very important to better understand the drug resistance mechanisms that prevent the effecttiveness of today’s treatment methods, to develop new treatment methods and the determination of molecular characterization. In recent years, research has focused on methods containing pathway analysis within the scope of developing targeted therapy strategies for ovarian cancer. In this context, the activity status of onco-proteins in pathways such as WNT, Hedgehog and NOTCH signaling pathways were evaluated. In addition, the innovative treatment efficiency of Nanog protein and photodynamic therapy options on ovarian cancer has been detailed in recent years. It will be possible to increase the quality of the healing process of ÖZET Kanser, kontrol edilemeyen hücre bölünmeleri sonucunda doku ve organlarda meydana gelen hasarların bütünüdür. Farklı görülme sıklığı ve ölüm oranları ile ilişkili birçok kanser türü vardır. Yumurtalık kanseri dünya genelinde yaygınlık olarak yedinci sırada gelmekte olup kansere bağlı ölüm nedeni olarak beşinci sırada gelmektedir. Kanser türlerinin kendi içinde standart tedavi yöntemleri olmasına rağmen bireyler bu tedavi yöntemlerine farklı cevaplar vermektedir. Geliştirilen tüm tedavi stratejilerine rağmen yumurtalık kanseri hala yüksek ölüm oranına sahiptir. Bu sebeple günümüzdeki tedavi yöntemlerinin etkinliğini engelleyen ilaca karşı oluşan direnç mekanizmalarını kavramak, yeni tedavi yöntemleri geliştirmek ve moleküler karakterizasyonunu belirlemek oldukça önemlidir. Son yıllarda oldukça gündeme gelen yumurtalık kanserinde hedefe yönelik terapi stratejileri geliştirilmesi kapsamında yolak analizleri üzerine odaklanılmıştır. Bu kapsamda onkoproteinlerin WNT, Hedgehog ve NOTCH sinyal yolağı gibi yolaklardaki etkinlik durumları değerlendirilmiştir. Ayrıca, son yıllarda etkinliği belirlenmiş Nanog proteini ve fotodinamik tedavi seçeneklerinin yumurtalık kanseri üzerindeki yenilikçi tedavi etkinliği detaylandırılmıştır. Yumurtalık kanseri tedavisinde mevcut ve geliştirilmesi Türk Hijyen ve Deneysel Biyoloji Dergisi 1Ankara Üniversitesi Biyoteknoloji Enstitüsü, Ankara Geliş Tarihi / Received : Kabul Tarihi / Accepted : İletişim / Corresponding Author : Demet CANSARAN DUMAN Ankara Üniversitesi, Biyoteknoloji Enstitüsü Merkez Laboratuvarı, Tandoğan / Ankara Türkiye E-posta / E-mail : dcansaran@yahoo.com 24.02.2021 16.04.2021 Çolak B, Cansaran Duman D, Yılmaz E. Yumurtalık kanserinde hedefe yönelik te","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70677085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.02256
Gülsen Topaktaş, Emine Çetin Aslan, Hüseyin Aslan
{"title":"Evaluation of Certified Health Education Programs Under the Coordination of The Türkiye Ministry of Health by The Participants","authors":"Gülsen Topaktaş, Emine Çetin Aslan, Hüseyin Aslan","doi":"10.5505/turkhijyen.2022.02256","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.02256","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.06641
G. Akcay, N. Derin
Objective: Stress causes neuropsychiatric diseases such as depression and anxiety in the person by affecting emotions, opinions and behaviors. In this study, the aim was to investigate the therapeutic effects of tDCS on depression and anxiety disorders that occur after sub-chronic stress. Methods: 40 male Wistar rats weighing 290-310 g were divided into four groups as control, tDCS, stress and stress+tDCS. Restraint stress was applied for 7 days (2 h/day) as a stress model. Rats in the tDCS and stress+tDCS groups were performed the stimulation of 1 mA anodal tDCS for three consecutive days from the 5th day of stress exposure. On the 7th day of the experiment, the anxiety and depression behaviors of the rats were evaluated ANKSIYETE BOZUKLUKLARI indicator of anxiety (p<0.001). In the stress + tDCS group compared to the control group, there was a significant decrease in the data indicator of anxiety following tDCS application (p <0.05), but no significant difference was found in the results of locomotor activity. Conclusion: In conclusion, our data showed that tDCS stimulation may have an effect on sub-chronic stress-induced anxiety and
{"title":"The Effects of tDCS on Depression and Anxiety Disorders Induced by Sub-Chronic Stress","authors":"G. Akcay, N. Derin","doi":"10.5505/turkhijyen.2022.06641","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.06641","url":null,"abstract":"Objective: Stress causes neuropsychiatric diseases such as depression and anxiety in the person by affecting emotions, opinions and behaviors. In this study, the aim was to investigate the therapeutic effects of tDCS on depression and anxiety disorders that occur after sub-chronic stress. Methods: 40 male Wistar rats weighing 290-310 g were divided into four groups as control, tDCS, stress and stress+tDCS. Restraint stress was applied for 7 days (2 h/day) as a stress model. Rats in the tDCS and stress+tDCS groups were performed the stimulation of 1 mA anodal tDCS for three consecutive days from the 5th day of stress exposure. On the 7th day of the experiment, the anxiety and depression behaviors of the rats were evaluated ANKSIYETE BOZUKLUKLARI indicator of anxiety (p<0.001). In the stress + tDCS group compared to the control group, there was a significant decrease in the data indicator of anxiety following tDCS application (p <0.05), but no significant difference was found in the results of locomotor activity. Conclusion: In conclusion, our data showed that tDCS stimulation may have an effect on sub-chronic stress-induced anxiety and","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.06982
Şule Aybüke Yavuz, Ü. Gül
Biotechnology is an interdisciplinary science that aims to produce products of high economic value using biological materials. Fungi are used as a source in many industrial processes such as the production of enzymes, vitamins, polysaccharides, polyhydric alcohols, pigments, lipids and glycolipids. These products are potentially valuable in terms of biotechnological studies as well as the economic value provided by their commercial production. The first biotechnological products include alcoholic beverages such as wine and beer also, basic nutrients such as bread and cheese produced by the use of yeast and fungi. Especially with the discovery of the first antibiotic, the possibilities of using fungi for the production of drugs and similar substances in health biotechnology have been started to be investigated. Fungal secondary metabolites are extremely important for our health and nutrition and also have a significant economic impact. In recent years, modified organisms have been used in biotechnological production as a result of the use of techniques developed by molecular biology and genetic studies. Today, there has been a significant increase in production and marketing activities for microbial enzymes and pharmaceutical üretimi ürünler ticari üretimleriyle mantarlar, sağlık products produced by recombinant DNA technology, including yeasts and other fungi as hosts. Today, fungal biology is an important participant in the global industry. Genomic and proteomic studies on newly discovered species are ongoing. The aim of this study is to examine the information in the literature about the use of fungi in health biotechnology. According to the results of this study, yeasts and fungi are widely used in health biotechnology, especially in the production of pharmaceutical products. Fungi and yeasts are used in the production of various pharmaceutical products such as antitumor and antimicrobial drug active substances, vaccines and hormones. In addition, it has been determined that new fungi and yeast species developed using recombinant DNA technologies can be used both more efficiently and effectively. In addition, the existence of newly isolated yeast and fungi species with many features not fully identified is promising for the production of new products in
生物技术是一门跨学科的科学，旨在利用生物材料生产高经济价值的产品。真菌在许多工业过程中被用作原料，如酶、维生素、多糖、多羟基醇、色素、脂类和糖脂类的生产。这些产品在生物技术研究方面具有潜在的价值，并因其商业化生产而具有经济价值。第一批生物技术产品包括葡萄酒和啤酒等酒精饮料，以及利用酵母和真菌生产的面包和奶酪等基本营养物质。特别是随着第一种抗生素的发现，人们开始研究在卫生生物技术中利用真菌生产药物和类似物质的可能性。真菌次生代谢物对我们的健康和营养极其重要，也具有重大的经济影响。近年来，由于使用分子生物学和遗传学研究开发的技术，转基因生物已被用于生物技术生产。今天，微生物酶和药物 retimi rnler ticari retimleriyle mantarlar, sağlık重组DNA技术生产的产品，包括酵母和其他真菌作为宿主，生产和销售活动显著增加。今天，真菌生物学是全球工业的重要参与者。对新发现物种的基因组学和蛋白质组学研究正在进行中。本研究的目的是检查有关真菌在卫生生物技术中使用的文献信息。根据本研究结果，酵母和真菌在卫生生物技术，特别是在制药产品的生产中有着广泛的应用。真菌和酵母用于生产各种医药产品，如抗肿瘤和抗菌药物活性物质、疫苗和激素。此外，已经确定使用重组DNA技术开发的新真菌和酵母物种可以更有效地利用。此外，新分离的酵母和真菌物种的存在，其许多特征尚未完全确定，为生产新产品提供了希望
{"title":"The use of fungi for the production of biotechnological products in the field of health","authors":"Şule Aybüke Yavuz, Ü. Gül","doi":"10.5505/turkhijyen.2022.06982","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.06982","url":null,"abstract":"Biotechnology is an interdisciplinary science that aims to produce products of high economic value using biological materials. Fungi are used as a source in many industrial processes such as the production of enzymes, vitamins, polysaccharides, polyhydric alcohols, pigments, lipids and glycolipids. These products are potentially valuable in terms of biotechnological studies as well as the economic value provided by their commercial production. The first biotechnological products include alcoholic beverages such as wine and beer also, basic nutrients such as bread and cheese produced by the use of yeast and fungi. Especially with the discovery of the first antibiotic, the possibilities of using fungi for the production of drugs and similar substances in health biotechnology have been started to be investigated. Fungal secondary metabolites are extremely important for our health and nutrition and also have a significant economic impact. In recent years, modified organisms have been used in biotechnological production as a result of the use of techniques developed by molecular biology and genetic studies. Today, there has been a significant increase in production and marketing activities for microbial enzymes and pharmaceutical üretimi ürünler ticari üretimleriyle mantarlar, sağlık products produced by recombinant DNA technology, including yeasts and other fungi as hosts. Today, fungal biology is an important participant in the global industry. Genomic and proteomic studies on newly discovered species are ongoing. The aim of this study is to examine the information in the literature about the use of fungi in health biotechnology. According to the results of this study, yeasts and fungi are widely used in health biotechnology, especially in the production of pharmaceutical products. Fungi and yeasts are used in the production of various pharmaceutical products such as antitumor and antimicrobial drug active substances, vaccines and hormones. In addition, it has been determined that new fungi and yeast species developed using recombinant DNA technologies can be used both more efficiently and effectively. In addition, the existence of newly isolated yeast and fungi species with many features not fully identified is promising for the production of new products in","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5505/turkhijyen.2022.57983
Nihan Çeken, Hülya Duran, Tuğba Kula Atik
Objective: Bacteremia is a serious condition that causes hospitalization and increases mortality. Gram negative bacteria are frequently isolated as causative agents. The aim of this study was to evaluate the distribution and antimicrobial resistance of the gram negative bacteria isolated from blood cultures in our hospital. Methods: Gram negative bacteria isolated from blood culture samples sent to the microbiology laboratory between 2016-2019 were retrospectively analyzed. The blood cultures were performed by BacT/ALERT 3D (bioMérieux, France) and Render-BC128 (China). Bacterial identification and antibiotic susceptibility tests were performed using conventional methods and BD Phoenix 100 (BD Phoenix System, Beckton Dickinson, ABD) automated systems. Results: A total of 10,315 blood cultures obtained from hospitalized patients between (95.4%), Pseudomonas aeruginosa to ciprofloxacin (26.1%). The most susceptible antibiotics were carbapenems and amikasin for Enterobacterales , amikasin and gentamycin for P. aeruginosa , amikasin and trimethoprim-sulfamethaxazole for A.baumannii . Conclusion: In our study, the frequency of gram-negative bacteria growth in blood culture was found to be 27.5%, similar to literature data. It was observed that the determined bacterial distribution was compatible with different studies. Blood culture is the most important test in the diagnosis of bacteremia and in directing the right treatment. The distribution of microorganisms isolated in bacteremia and their antibiotic resistance rates changes by center. For this reason, it was think that each hospital should determine its own data at regular intervals and guide antibiotic selection according to these results.
{"title":"Distribution of gram negative bacteria isolated from blood culture and antibiotic resistance rates","authors":"Nihan Çeken, Hülya Duran, Tuğba Kula Atik","doi":"10.5505/turkhijyen.2022.57983","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.57983","url":null,"abstract":"Objective: Bacteremia is a serious condition that causes hospitalization and increases mortality. Gram negative bacteria are frequently isolated as causative agents. The aim of this study was to evaluate the distribution and antimicrobial resistance of the gram negative bacteria isolated from blood cultures in our hospital. Methods: Gram negative bacteria isolated from blood culture samples sent to the microbiology laboratory between 2016-2019 were retrospectively analyzed. The blood cultures were performed by BacT/ALERT 3D (bioMérieux, France) and Render-BC128 (China). Bacterial identification and antibiotic susceptibility tests were performed using conventional methods and BD Phoenix 100 (BD Phoenix System, Beckton Dickinson, ABD) automated systems. Results: A total of 10,315 blood cultures obtained from hospitalized patients between (95.4%), Pseudomonas aeruginosa to ciprofloxacin (26.1%). The most susceptible antibiotics were carbapenems and amikasin for Enterobacterales , amikasin and gentamycin for P. aeruginosa , amikasin and trimethoprim-sulfamethaxazole for A.baumannii . Conclusion: In our study, the frequency of gram-negative bacteria growth in blood culture was found to be 27.5%, similar to literature data. It was observed that the determined bacterial distribution was compatible with different studies. Blood culture is the most important test in the diagnosis of bacteremia and in directing the right treatment. The distribution of microorganisms isolated in bacteremia and their antibiotic resistance rates changes by center. For this reason, it was think that each hospital should determine its own data at regular intervals and guide antibiotic selection according to these results.","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70676968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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