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Main forestry and management indices of pine (Pinus sylvestris L.) stands involving beech (Fagus sylvatica L.) in composition of Ukrainian Roztochchia 乌克兰roztochia组成中涉及山毛榉(Fagus sylvatica L.)的松树林分的主要林业和管理指标
Q3 Agricultural and Biological Sciences Pub Date : 2021-06-01 DOI: 10.2478/ffp-2021-0009
Myklush Yuriy, Myklush Stepan, Havryliuk Serhii, Savchyn Volodymyr
Abstract As of 2019, the total area of forest stands with the varying proportion of Scots pine (Pinus sylvestris L.) and beech (Fagus sylvatica L.) in composition on the Ukrainian Roztochchia was 11,251.3 ha and the area of pine stands involving European beech in composition was 6879.5 ha. Pine stands involving beech in composition are formed on the fresh, moist and boggy relatively pure and relatively rich soil condition. Pine forest stands (average share of pine exceeds 56%), which include beech in composition occupy more than 80% of the total area of interest. Mature and overmature forest stands dominate most of the landscape. More than 85% of stands are characterized by an average relative completeness. Undergrowth of deciduous tree species, which is mainly formed by European beech or hornbeam (Carpinus betulus L.) and rarely by common oak (Quercus robur L.) or sycamore maple (Acer pseudoplatanus), is available under a canopy of middle-aged, ripening and mature stands. The study points to the need to rethink forest management approaches and eventually to use such methods of forest cuttings that ensure natural regeneration not only of a European beech and common oak, but primarily of a pine. The practice of creating pine plantations in such conditions favourable for its growth and development should be used only in those cases when it is impossible to achieve natural regeneration of pine.
截至2019年,乌克兰roztochia上不同比例组成的苏格兰松(Pinus sylvestris L.)和山毛榉(Fagus sylvatica L.)林分总面积为11,251.3 ha,组成欧洲山毛榉的松林面积为6879.5 ha。以山毛榉为主要成分的松林是在相对纯净、肥沃的新鲜湿润沼泽土壤条件下形成的。包括山毛榉在内的松林林分(松树平均占比超过56%)占研究区总面积的80%以上。成熟和过成熟的林分占据了大部分景观。超过85%的林分以平均相对完整度为特征。在中年、成熟和成熟林分的树冠下,有落叶树种,主要由欧洲山毛榉或角木(Carpinus betulus L.)组成,很少有普通橡树(Quercus robur L.)或梧桐槭(Acer pseudoplatanus)组成。该研究指出,需要重新考虑森林管理方法,并最终采用这种森林采伐方法,不仅确保欧洲山毛榉和普通橡树的自然再生,而且主要是松树的自然再生。在这种有利于其生长和发展的条件下建立松树种植园的做法,只应在不可能实现松树自然再生的情况下使用。
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引用次数: 2
Trends in forest fuel accumulation in pine forests of Kyiv Polissya in Ukraine 乌克兰基辅波利西亚松林的森林燃料积累趋势
Q3 Agricultural and Biological Sciences Pub Date : 2021-06-01 DOI: 10.2478/ffp-2021-0013
R. Hurzhii, P. Yavorovskyi, S. Sydorenko, V. Levchenko, Olexandr M. Tyshchenko, A. P. Tertyshnyi, B. Yakubenko
Abstract At present, forest fire research is becoming especially relevant in Ukraine. This study examines patterns of forest fuel accumulation in pine (Pinus sylvestris L.) stands that grow in different soil conditions with different pine stand structure. To estimate the load of forest fuel of different fractions, a combined methodology was used: the weighing method and the FIREMON (fuel load estimation) method. It was found that increase in surface forest fuel loads is not directly proportional to forest stands’ age. Fractional size distribution, capacity and loads of forest fuel depend on several factors, among which the greatest role is played by forestry characteristics of the pine stand. It was determined that in the forest site conditions of type C (fairly rich soils) in Kyiv Polissya, the share of forest litter compared to pine stands that grow in poor soil conditions (A) is smaller, ranging from 41% to 76% of the total forest fuel load. The mass proportion of the duff layer varies from 15% in young forest stands to 43% in mature stands. It was established that changes in forest fuel fractions for 1, 10, 100 and 1000 hours varied insignificantly with age rate. The share of substratum woody debris of 10 and 100 hours was insignificant and depended more on the forestry treatment regime on these sites. The mass proportion of coarse woody debris (1000 hours) was also insignificant, varying from 0% to 5.9% of the total load of surface fuel.
目前,乌克兰的森林火灾研究正变得尤为重要。本文研究了生长在不同土壤条件下、不同林分结构下的松林燃料积累模式。采用称重法和燃料负荷估算法(firremon)相结合的方法对不同馏分的森林燃料负荷进行估算。研究发现,地表林分燃料负荷的增加与林分年龄不成正比。森林燃料的粒度分布、容量和负荷取决于几个因素,其中松林的林业特性起着最大的作用。经确定,在Kyiv Polissya C型(相当肥沃的土壤)的森林立地条件下,与生长在贫瘠土壤条件(A)的松林相比,森林凋落物的份额较小,占森林总燃料负荷的41%至76%。枯草层的质量比例从幼林的15%到成熟林的43%不等。结果表明,1、10、100和1000 h的森林燃料组分随龄期变化不显著。10小时和100小时的基材木屑所占的比例不显著,主要取决于这些立地的林业处理制度。粗木屑的质量比例(1000小时)也不显著,占地面燃料总负荷的0% ~ 5.9%。
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引用次数: 3
Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza 有夏松露外生菌根与无夏松露外生菌根树木根系细菌群落比较
Q3 Agricultural and Biological Sciences Pub Date : 2021-06-01 DOI: 10.2478/ffp-2021-0011
Marta Siebyła, I. Szyp-Borowska
Abstract In this study, we examined the effect of the presence of mycorrhiza and ascomata of summer truffle (Tuber aestivum) on the bacterial composition of roots from small trees growing in selected sites of the Nida Basin. Qualitative DNA sequencing methods such as Sanger and next-generation sequencing (NGS) were used. The Sanger method revealed different bacterial species compositions between the samples where summer truffle ascomata was recorded and control samples. Five genera of bacteria could be distinguished: Bacillus, Erwinia, Pseudomonas, Rahnella and Serratia, among which the most numerous were Pseudomonas (Gammmaproteobacteria class) at 32.9%. The results obtained by the NGS method also showed differences in species composition of the bacteria depending on the study sample. Seven genera of bacteria were distinguished: Rhizorhabdus, Methylotenera, Sphingomonas, Nitrosospira, Streptomyces, Methyloceanibacter and Niastella, which dominated in roots from the truffle sites. Telmatobacter, Roseiarcus, Granulicella, Paludibaculum, Acidipila, Acidisphaera and Aliidongia dominated in roots from the control sites. With the NGS method, it is possible to identify the microbiome of a whole root, while only a root fragment can be analysed by the Sanger method. These results extend the scope of knowledge on the preferences of certain groups of bacteria associated with truffles and their influence on the formation of ascomata in summer truffles. Our results may also be useful in selecting and monitoring sites that promote ascomata of Tuber aestivum.
摘要在本研究中,我们研究了夏季松露(Tuber aestivum)菌根和ascomata的存在对生长在Nida盆地的小树根系细菌组成的影响。采用Sanger和下一代测序(NGS)等定性DNA测序方法。桑格法显示,夏季松露ascomata记录样品与对照样品的细菌种类组成不同。鉴定出芽孢杆菌属、欧文菌属、假单胞菌属、拉赫菌属和沙雷菌属5属,其中以假单胞菌属(γ -变形菌纲)最多,占32.9%。通过NGS方法获得的结果还显示,细菌的种类组成因研究样本而异。鉴定出7属细菌:Rhizorhabdus、Methylotenera、Sphingomonas、Nitrosospira、Streptomyces、methyloeanbacter和Niastella,在松露部位的根中占主导地位。在对照区,末端杆菌、Roseiarcus、Granulicella、Paludibaculum、Acidipila、Acidisphaera和Aliidongia在根中占主导地位。使用NGS方法,可以识别整个根的微生物组,而Sanger方法只能分析根的片段。这些结果扩大了对与松露有关的某些细菌群的偏好及其对夏季松露中ascomata形成的影响的认识范围。我们的结果也可能有助于选择和监测促进块茎鳞片形成的位点。
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引用次数: 1
The attempt to assess the fire risk of non-forest terrestrial ecosystems of Biebrza National Park – A case study Biebrza国家公园非森林陆地生态系统火灾风险评估的尝试-一个案例研究
Q3 Agricultural and Biological Sciences Pub Date : 2021-06-01 DOI: 10.2478/ffp-2021-0017
R. Szczygieł, M. Kwiatkowski, Bartłomiej Kołakowski
Abstract On 19th April, the largest wildfire of the entire history of Biebrza National Park broke out and consumed 5526 ha, mostly overdried grassland with sedges and reeds of Biebrza marshes. The very rapid spread of the fire in the open space, with the blowing wind changing directions and the inaccessibility of the area for fire-fighting vehicles were the main reasons of this third largest wildfire in Poland, after the tragic forest fires in Kuźnia Raciborska (9060 ha burnt) and Potrzebowice (5600 ha burnt) in the memorable year of 1992. After this event, activities were undertaken to develop a fire protection plan for the Biebrza National Park. It took into account an innovative approach during the analysis of the existing fire risk, primarily regarding the non-forest terrestrial ecosystems composed of herbaceous vegetation, which constitutes as much as 61.2% of the park’s area, and to propose protection methods adequate to the threat. The work was completed in the framework of the project entitled ‘Development of the method for assessing the fire risk of non-forest ecosystems and the principles of fire protection for the Biebrza National Park – stage I,’ commissioned for the Forest Research Institute by the Biebrza National Park, financed by the State Forests from the forest fund, in accordance with the contract EZ.0290.1.24.2020. The article presents a preliminary method of classifying the fire risk of non-forest ecosystems, considering the occurrence of the fires in the Biebrza National Park in the years 2007–2020 and the type of vegetation burnt. This method, after supplementary field tests planned in 2021, will enable evaluation of the fire risk, which shall be a premise for the development of a fire protection plan.
4月19日,Biebrza国家公园发生了历史上最大的野火,烧毁了5526公顷,主要是Biebrza沼泽的芦苇和莎草过度干燥的草地。大火在空地上迅速蔓延,风向改变,消防车无法进入该地区,这是波兰第三大野火的主要原因,仅次于1992年在Kuźnia Raciborska(烧毁9060公顷)和Potrzebowice(烧毁5600公顷)发生的悲惨森林火灾。在这次事件之后,开展了为Biebrza国家公园制定消防计划的活动。在分析现有火灾风险时,采用了一种创新的方法,主要针对占公园面积61.2%的草本植被组成的非森林陆地生态系统,并提出了适当的保护措施。这项工作是在“开发非森林生态系统火灾风险评估方法和别布尔扎国家公园防火原则-第一阶段”项目框架内完成的,该项目由别布尔扎国家公园委托森林研究所完成,由国家森林基金资助,根据EZ.0290.1.24.2020合同。本文结合2007-2020年Biebrza国家公园的火灾发生情况和被烧毁的植被类型,提出了一种初步的非森林生态系统火灾风险分类方法。在计划于2021年进行补充现场试验之后,该方法将能够评估火灾风险,这将是制定消防计划的前提。
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引用次数: 2
Zakour Forest fire risk map assessment in the commune of Mamounia (Mascara, Algeria) 阿尔及利亚马穆尼亚(Mascara)社区Zakour森林火灾风险地图评估
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/ffp-2021-0003
D. Anteur, A. Benaradj, Youcef Fekir, Djillali Baghdadi
Abstract The great forest of Zakour is located north of the commune of Mamounia (department of Mascara). It is considered the lung of the city of Mascara, covers an area of 126.8 ha. It is a forest that is subject to several natural and human constraints. Among them, the fires are a major danger because of their impacts on forest ecosystems. The purpose of this work is to develop a fire risk map of the Zakour Forest through the contribution of geomatics according to natural and anthropogenic conditions (human activities, agglomeration, agricultural land) while integrating information from ground on the physiognomy of the vegetation. For this, the creation of a clearer fire risk map to delimit the zones potentially sensitive to forest fires in the forest area of Zakour. This then allows good implementation of detection management plans, for better prevention and decision-making assistance in protecting and fighting forest fires.
扎库尔大森林位于马蒙尼亚(睫毛膏省)公社的北部。它被认为是睫毛膏之城的肺,占地126.8公顷。这是一片受到自然和人为限制的森林。其中,火灾是一个主要的危险,因为它们对森林生态系统的影响。这项工作的目的是根据自然和人为条件(人类活动、聚集、农业用地),通过地理信息学的贡献,开发Zakour森林的火灾风险地图,同时整合来自地面的植被地貌信息。为此,创建一个更清晰的火灾风险地图,以划定Zakour森林地区可能对森林火灾敏感的区域。这样就可以很好地实施探测管理计划,以便在保护和扑灭森林火灾方面更好地预防和决策协助。
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引用次数: 0
First report of the white rotting fungus Sarcodontia crocea in Armenia 亚美尼亚首次发现白色腐烂真菌crocea
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/ffp-2021-0006
A. Gasparyan, A. Szczepkowski
Abstract The Sarcodontia crocea (Schwein.) Kotl. is recorded for the first time from Armenia. The specimen has been collected from the old fallen branch of apple tree (Malus sp.) and known from the single locality, the private orchard at the Vahagni village, Lori province. Herein, the collected specimen, habitat and threats are briefly described. Further inclusion to the Red Data Book of Armenia is recommended.
[摘要]crocea (Schwein.)Kotl。第一次记录在亚美尼亚。该标本采集自一棵老苹果树(Malus sp.)的倒下树枝,并在洛里省Vahagni村的一个私人果园中被发现。本文简要介绍了所收集的标本、栖息地和威胁。建议将其进一步列入亚美尼亚红色数据手册。
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引用次数: 0
The influence of forest types on manganese content in soils 不同森林类型对土壤锰含量的影响
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/ffp-2021-0001
P. Michopoulos, Marios Kostakis, N. Thomaidis, I. Pasias
Abstract The concentrations of available and total Mn were determined in the soils of three different forest ecosystems, i.e. a maquis forest, a beech forest and fir one. The concentrations of total Mn in the deeper mineral horizons reflected the type of patent material, but in the surface layers, the more acidic soil (in the beech forest) had the higher concentrations. This was due to the high concentrations of Mn in the standing leaves and litterfall of beech trees, which brought about high litterfall fluxes of Mn in that forest. However, the concentrations of (DTPA) available Mn was significantly higher in the soil under beech only in the 0–10 cm layer, whereas the fast decomposition of organic matter in the Mediterranean zone resulted in higher concentrations of available Mn in the Ofh soil horizon of the maquis plot. The available Mn did not correlate with soil pH. These findings mean that high concentrations of available Mn do not always entail higher uptake. The soil pH played a predominant role for the high concentrations in the vegetation of the beech forest.
摘要测定了3种不同森林生态系统(即猕猴林、山毛榉林和冷杉林)土壤中有效态和全态Mn的浓度。在较深的矿物层中,总Mn的浓度反映了专利物质的类型,但在表层,酸性越强的土壤(山毛榉林)中总Mn的浓度越高。这是由于山毛榉立叶和凋落物中Mn浓度高,导致该森林凋落物Mn通量大。然而,山毛榉下土壤(DTPA)有效锰浓度仅在0-10 cm层显著较高,而地中海地区有机质的快速分解导致maquis地块Ofh土壤层有效锰浓度较高。有效锰与土壤ph值没有相关性。这些发现意味着高浓度的有效锰并不一定意味着较高的吸收量。土壤pH对山毛榉林植被的高浓度起主导作用。
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引用次数: 1
Forest shelter belts in organic agricultural landscape: structure of biodiversity and their ecological role 有机农业景观中的森林防护林带:生物多样性结构及其生态作用
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/ffp-2021-0005
V. Lavrov, Nataliіa Miroshnyk, Tatіana Grabovska, T. Shupova
Abstract The aim of this article is to assess the structure of biodiversity of field protective forest shelter belts to determine the directions of increasing their reclamation and conservation potential in the organic agricultural landscape. Methods of comparative ecology (synecological approach, assessment of α-diversity), forestry, geobotany, ornithology and statistical analysis are used. Changes in species diversity of plants and birds in forest shelter belts around organic fields are characterized. It is established that plantations with developed undergrowth and under-storey are transformed due to the loss of up to 43.5% of trees, liquefaction of the edificatory tier. Transformer species with a wide phytocenotic range predominate in the tree stands. The grass tier is dominated by ruderants (36.5%) and adventive species (24.5%). The share (60%) of species with a mixed life strategy indicates changes in moisture conditions and soil trophism. A 2.4% of shade-loving plants and the presence of 14.7% nitrophils indicate an imbalance in the structure of forest shelter belts. Depletion of species composition, ecological and trophic structure of bird groups testify to the decrease in the capacity of forest shelter belts and their statio diversity, reduction of protective, environment-creating functions and other ecosystem services. In the three-tiered dense forest shelter belts with developed undergrowth and understorey, 10–27 species of birds nest. In general, avifauna is represented by 2 ecological groups (93.1% dendrophiles, 6.9% sclerophiles), divided into 5 types of nesting strategy (with a predominance of hollow-nests 37.0–53.3%), and 5 types of feeding (with a predominance of entomophagous 70.4–90.0%). Twenty-nine species have been identified on nesting, 27 of which are subject to protection at the level of international agreements. There are no adventive species, the index of synanthropization of avifauna is high – 0.85–1.0. For organic technologies to increase the potential of biological protection of fields from entomological pests by attracting insectivorous birds is relevant.
摘要本文旨在评价大田防护林防护林带的生物多样性结构,以确定提高其在有机农业景观中的复垦和保护潜力的方向。采用比较生态学(协同学、α-多样性评价)、林学、地植物学、鸟类学、统计分析等方法。研究了有机农田周围森林防护林带植物和鸟类物种多样性的变化特征。已经确定,由于高达43.5%的树木的损失,熏蒸层的液化,具有发达的林下和下层的人工林发生了转变。具有广泛植物生长范围的变形种在林分中占主导地位。禾本科层以生虫(36.5%)和外来物种(24.5%)为主。具有混合生活策略的物种的份额(60%)表明水分条件和土壤营养性的变化。2.4%的喜阴植物和14.7%的硝化剂表明森林防护林带结构不平衡。鸟类种群的物种组成、生态和营养结构的耗竭证明了森林防护林带及其站点多样性的能力下降,保护、创造环境的功能和其他生态系统服务功能的减少。在林下和林下发育的三层密林防护林带中,有10 ~ 27种鸟类筑巢。总体上,鸟类有2个生态类群(93.1%为嗜树动物,6.9%为嗜硬动物),分为5种筑巢策略(以空心巢为主37.0 ~ 53.3%)和5种摄食策略(以食虫为主70.4 ~ 90.0%)。目前已发现有29种鸟类在筑巢,其中27种受到国际协议级别的保护。无外来种,鸟类共化指数高,为0.85 ~ 1.0。对于有机技术来说,通过吸引食虫鸟类来增加对昆虫害虫的生物保护潜力是相关的。
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引用次数: 3
Do pheromone trapping always reflect Ips typographus (L.) population level? A study from the Tatra National Park in Poland 信息素捕获是否总能反映印型Ips (L.)种群水平?一项来自波兰塔特拉国家公园的研究
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/ffp-2021-0004
W. Grodzki
Abstract Pheromone traps are used for monitoring I. typographus populations in Norway spruce stands of the Tatra National Park (TPN) in Poland. The presented study is based on the set of pheromone traps of precisely known location (23) located in the whole area of the TPN and operated continuously in 2010–2019. The data on the captures of beetles were compared with two kinds of data concerning the mortality: the area covered by standing dead trees (airborne photographs) in the no-intervention zone, and the volume of trees infested by bark beetles processed in the active protection zone. No relationship was found between the mean numbers of beetles captured yearly in all pheromone traps in the whole TPN area and the volume of infested trees removed from the stands in the active protection zone. The captures in the two selected study areas were correlated with the area of spots with dead trees in the 500 m circle around the traps, however, this correlation is not statistically significant. There is no relation of captures to the volume of processed infested trees. The captures decreased in the growing seasons after the wind damage, and increased markedly after the drought started in 2015. The results of pheromone trapping are affected by several factors, as wind damage and defence potential of trees resulting from their physiological status. Pheromone traps represent valuable source of information about the bark beetle I. typographus population dynamics, although the collected data do not enable direct definition of its population level, especially in the protected areas with different and unstable (changed in 2017) approach to the protection of stands. As most of the information on beetles is captured in the first half of the growing season, the data collected till the end of July are sufficient for monitoring purposes; thus, the trapping should be reduced to the period May–July.
在波兰塔特拉国家公园(TPN)的挪威云杉林内,采用信息素诱捕器监测印字蝽种群。本研究基于一组精确已知位置的信息素陷阱(23个),这些陷阱位于TPN的整个区域,并在2010-2019年连续运行。将甲虫捕获数据与两种有关死亡率的数据进行了比较:无干预区立死树覆盖面积(航空照片)和主动保护区处理的树皮甲虫侵染树木数量。全保护区所有信息素诱捕器的年平均捕获甲虫数与主动保护区林分的被侵染树木移走量无明显关系。所选两个研究区域的捕获量与捕集器周围500 m范围内死树点的面积相关,但这种相关性不具有统计学意义。捕获量与处理过的受感染树木的数量没有关系。在风灾后的生长季节,捕获量下降,在2015年干旱开始后显著增加。信息素诱捕的效果受多种因素的影响,如树木的风害和树木生理状态造成的防御能力。信息素陷阱是树皮甲虫种群动态的宝贵信息来源,尽管收集到的数据不能直接定义其种群水平,特别是在不同和不稳定(2017年发生变化)的林分保护方法的保护区。由于甲虫的大部分资料是在生长季节的前半段收集的,因此收集到7月底的数据足以用于监测目的;因此,捕集应减少到5 - 7月。
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引用次数: 2
In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis 圣诞仙人掌(Schlumbergera truncata, Haw.)离体植株再生研究间接形态发生
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/ffp-2021-0007
O. Chornobrov, S. Bilous
Abstract Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl2 for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).
摘要Schlumbergera truncata (Haw.)属植物在离体条件下,通过间接形态发生的方法,从茎段中获得Moran,并进行繁殖和生根。还测定了光照条件、营养培养基组成对愈伤组织形成的一致性和频率的影响。这些研究是在2016-2018年进行的。建立了0.1% HgCl2 7 ~ 8 min有效灭菌(90%以上)的模式。在MS (Murashige and Skoog 1962)培养基中添加1.0 mg/l BAP(6-苄氨基嘌呤)和0.3 mg/l NAA(1-萘基乙酸),并对外植体进行大量切割,在此条件下,获得了茎节段诱导愈伤组织形成的最佳条件(诱导率90%以上,生长显著)。光照强度为2.0-3.0 klx时,愈伤组织呈浓稠的墨绿色色素沉着,在恒温无光照条件下,愈伤组织呈浓稠疏松的暗黄色色素沉着。光照条件和营养培养基组成对愈伤组织形成频率的影响具有统计学意义。在添加2.0 mg/l BA的MS培养基上芽数最多。同时,在添加0.5 mg/l BA和0.5 mg/l激动素(60 d培养周期增殖因子- 8.8±0.6)的MS培养基上,观察到这些数量的芽增殖和根诱导。
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引用次数: 6
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Folia Forestalia Polonica, Series A
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