A. Anene, Eme Okories Okorie, Malachy N. O. Ajima, Janet Onyemaonwu
Dietary supplementation with herbal extract in the diet of fish has been noted to increase aquaculture production through the improvement of immunity and other physiological dysfunctions related to fish health. A feeding trial was conducted to assess the impact of incorporating varied concentrations of turmeric powder (TP) on haematological and biochemical responses in Clarias gariepinus fingerlings. Five test diets were composed as T1 ((control) (basal diet + 0 % TP)), T2 (basal diet + 1 % TP), T3 (basal diet + 2 % TP), T4 (basal diet + 3 % TP) and T5 (basal diet + 4 % TP). Fish specimens were apportioned into five groups in triplicate. Significantly lower (P<0.05) red blood cell count was found in those fed control diet compared with the other inclusion. Highest values of white blood cell count were noticed in T5 when compared with the control. There were significant differences in haemoglobin between T1 and T5. Highest values of serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, glucose and cholesterol level were found in T2 when compared with the control. TP can be used as feed supplement in the diet of C. gariepinus without any adverse effect on physiological responses of the fish.
{"title":"Dietary Supplement of Tumeric (Curcuma longa) Powder: Impact on Haematological and Biochemical Responses in Clarias gariepinus (Burchell, 1822) Fingerlings","authors":"A. Anene, Eme Okories Okorie, Malachy N. O. Ajima, Janet Onyemaonwu","doi":"10.4194/aquast714","DOIUrl":"https://doi.org/10.4194/aquast714","url":null,"abstract":"Dietary supplementation with herbal extract in the diet of fish has been noted to increase aquaculture production through the improvement of immunity and other physiological dysfunctions related to fish health. A feeding trial was conducted to assess the impact of incorporating varied concentrations of turmeric powder (TP) on haematological and biochemical responses in Clarias gariepinus fingerlings. Five test diets were composed as T1 ((control) (basal diet + 0 % TP)), T2 (basal diet + 1 % TP), T3 (basal diet + 2 % TP), T4 (basal diet + 3 % TP) and T5 (basal diet + 4 % TP). Fish specimens were apportioned into five groups in triplicate. Significantly lower (P<0.05) red blood cell count was found in those fed control diet compared with the other inclusion. Highest values of white blood cell count were noticed in T5 when compared with the control. There were significant differences in haemoglobin between T1 and T5. Highest values of serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, glucose and cholesterol level were found in T2 when compared with the control. TP can be used as feed supplement in the diet of C. gariepinus without any adverse effect on physiological responses of the fish.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46994179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Matvienko, Alexander Koziy, A. Didenko, Issak Sherman
Optimization of the process of sterlet feeding in recirculating aquaculture systems allows improving the physiological status and pre-spawning performance of fish. The addition of the organophosphorus compound Catosal based on butaphosphamide to pre-spawning feeds leads to an increase in glycogenogenesis in hepatocytes, which manifests itself as a decrease in cell and nucleus area (by 1.20 and 1.31 times), stabilization of nucleoplasmic ratios (0.28-0.25). Butaphosphamide also causes an increase in hemoglobin and serum protein concentrations by 6.1 g/L and 3.92 g/L, respectively. An increase in the live weight of sterlet by 98.0 g and an absolute increase in weight by 1.96 g associated with an improvement in hematological parameters was observed. The excess phosphorus in the form of butaphospham, which is not involved in the process of glycogenesis, is transported to gonads and stimulates vitellogenesis. The acceleration of oocyte development is determined upon reaching the optimal polarization coefficient (0.05≤Pc≤0.10, category II).
{"title":"Assessment of the Effect of Catosal on the Stimulation of Ovogenesis in Sterlet During Pre-spawning Period","authors":"N. Matvienko, Alexander Koziy, A. Didenko, Issak Sherman","doi":"10.4194/aquast583","DOIUrl":"https://doi.org/10.4194/aquast583","url":null,"abstract":"Optimization of the process of sterlet feeding in recirculating aquaculture systems allows improving the physiological status and pre-spawning performance of fish. The addition of the organophosphorus compound Catosal based on butaphosphamide to pre-spawning feeds leads to an increase in glycogenogenesis in hepatocytes, which manifests itself as a decrease in cell and nucleus area (by 1.20 and 1.31 times), stabilization of nucleoplasmic ratios (0.28-0.25). Butaphosphamide also causes an increase in hemoglobin and serum protein concentrations by 6.1 g/L and 3.92 g/L, respectively. An increase in the live weight of sterlet by 98.0 g and an absolute increase in weight by 1.96 g associated with an improvement in hematological parameters was observed. The excess phosphorus in the form of butaphospham, which is not involved in the process of glycogenesis, is transported to gonads and stimulates vitellogenesis. The acceleration of oocyte development is determined upon reaching the optimal polarization coefficient (0.05≤Pc≤0.10, category II).","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42074924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infectious Pancreatic Necrosis Virus (IPNV) and Viral hemorrhagic septicemia virus (VHSV) cause significant losses in the aquaculture industry. There have been few reports of the use of screening rainbow trout for antibodies against IPNV and VHSV as an epidemiological tool. Several ELISAs using a whole virus or recombinant IPNV and VHSV proteins have been described. In this study, a recombinant protein-based enzyme-linked immunosorbent assay (ELISA) for the detection of IPNV and VHSV antibodies in rainbow trout (Oncorhynchus mykiss) was evaluated. To develop recombinant protein-based enzyme-linked immunosorbent assays, a fragment containing the entire length of the gG gene of VHSV and VP2 of IPNV was amplified by PCR using the viruses' genomic RNA and cloned in pET-28a(+) plasmid. Recombinant structural viral proteins (rVP2 and rgG) were expressed in the Escherichia coli BL21 (DE3). The rgG was extracted and purified. 96-well plates were coated with VP2 and gG separately. For VHSV, Assay could detect until 1/15625 dilution in VHSV positive fish serum. For IPNV, Assays could detect until 1/3125 dilution in IPNV positive fish serum. These results show rgG and rVP2, used in ELISA, are more sensitive than virus neutralization tests.
{"title":"Detections of Rainbow Trout Antibodies to Infectious Pancreatic Necrosis Virus and Viral Hemorrhagic Septicemia Virus via a Recombinant Protein-Based Enzyme-Linked\u0000Immunosorbent Assay","authors":"C. Tamer, S. Gumusova, H. Albayrak","doi":"10.4194/aquast669","DOIUrl":"https://doi.org/10.4194/aquast669","url":null,"abstract":"Infectious Pancreatic Necrosis Virus (IPNV) and Viral hemorrhagic septicemia virus (VHSV) cause significant losses in the aquaculture industry. There have been few reports of the use of screening rainbow trout for antibodies against IPNV and VHSV as an epidemiological tool. Several ELISAs using a whole virus or recombinant IPNV and VHSV proteins have been described. In this study, a recombinant protein-based enzyme-linked immunosorbent assay (ELISA) for the detection of IPNV and VHSV antibodies in rainbow trout (Oncorhynchus mykiss) was evaluated. To develop recombinant protein-based enzyme-linked immunosorbent assays, a fragment containing the entire length of the gG gene of VHSV and VP2 of IPNV was amplified by PCR using the viruses' genomic RNA and cloned in pET-28a(+) plasmid. Recombinant structural viral proteins (rVP2 and rgG) were expressed in the Escherichia coli BL21 (DE3). The rgG was extracted and purified. 96-well plates were coated with VP2 and gG separately. For VHSV, Assay could detect until 1/15625 dilution in VHSV positive fish serum. For IPNV, Assays could detect until 1/3125 dilution in IPNV positive fish serum. These results show rgG and rVP2, used in ELISA, are more sensitive than virus neutralization tests.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48860032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rita N. Nairuti, S. N. Musyoka, Mourine J. Yegon, M. Opiyo
Aquaculture plays a major role in curbing malnutrition and food insecurity. Nonetheless, aquaculture sustainability is threatened by expensive fish feeds due to the overreliance on fish meal (FM) as the main source of protein. Fish meal is not only expensive but also scarce due to declining capture fisheries and competition from other animal feed producers. This has prompted research on potential FM replacers, amongst them the black soldier fly (BSF) larvae (Hermetia illucens). The BSF larvae can effectively convert organic wastes into a potential valuable feed source, and its high nutritional content (crude protein of up to 64% dry matter) is vital for fish feed formulation. Nevertheless, there are no documented studies on the complete replacement of FM in the diets of fish using BSF larvae. Therefore, the current study reviewed 107 research publications related to BSF larvae vis-a-visfish feeds production to build capacity for existing theories, identify gaps, and suggest new and further research directions, based on the previous studies available in the area of larvae production and utilization in aquaculture nutrition. The study results are expected to help farmers make an informed decision on how to reduce the cost of fish production, increase yields, thus promoting food security, livelihoods, and ecological balance.
{"title":"Utilization of Black Soldier Fly (Hermetia illucens Linnaeus) Larvae as a Protein Source for Fish Feed: A Review","authors":"Rita N. Nairuti, S. N. Musyoka, Mourine J. Yegon, M. Opiyo","doi":"10.4194/aquast697","DOIUrl":"https://doi.org/10.4194/aquast697","url":null,"abstract":"Aquaculture plays a major role in curbing malnutrition and food insecurity. Nonetheless, aquaculture sustainability is threatened by expensive fish feeds due to the overreliance on fish meal (FM) as the main source of protein. Fish meal is not only expensive but also scarce due to declining capture fisheries and competition from other animal feed producers. This has prompted research on potential FM replacers, amongst them the black soldier fly (BSF) larvae (Hermetia illucens). The BSF larvae can effectively convert organic wastes into a potential valuable feed source, and its high nutritional content (crude protein of up to 64% dry matter) is vital for fish feed formulation. Nevertheless, there are no documented studies on the complete replacement of FM in the diets of fish using BSF larvae. Therefore, the current study reviewed 107 research publications related to BSF larvae vis-a-visfish feeds production to build capacity for existing theories, identify gaps, and suggest new and further research directions, based on the previous studies available in the area of larvae production and utilization in aquaculture nutrition. The study results are expected to help farmers make an informed decision on how to reduce the cost of fish production, increase yields, thus promoting food security, livelihoods, and ecological balance.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47278603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Iqbal, M. Afzal, A. Yaqub, K. M. Anjum, K. Tayyab
Aquaculturists have been concentrating their efforts to design aquafeeds using agro by-products to reduce feed costs. However, the presence of a wide variety of antinutritional factors (ANFs) in plant materials is one of the major obstacles. For this purpose, a 2x2 factorial experiment was established to evaluate the suitability of canola meal with citric acid (CA) and phytase (PHY) supplementation for growth performance, nutrient digestibility and body composition of Labeo rohita fingerlings. Five isocaloric and isonitrogenous diets; D1 (control; without CA and PHY), D2 (CA 15 g/kg+PHY 1000 FTU/kg), D3 (CA 30 g/kg+PHY 1000 FTU/kg), D4 (CA 15 g/kg+PHY 2000 FTU/kg) and D5 (CA 30 g/kg+PHY 2000 FTU/kg) were fed to fish (mean initial weight 7.61±0.31 g) in triplicates for 90-days. The growth performance was significantly increased while feed conversion ratio was decreased with D3 compared to control and other CA and PHY supplemented groups (P<0.05). Furthermore, higher digestibility (%) of nutrients (dry matter, crude protein and ash) and improved whole-body composition was also exhibited by fish fed with D3 (P<0.05). Hence, the above results elucidate that supplementation of 30 g/kg CA with 1000 FTU/kg PHY could be a useful approach for improved growth performance, nutrients digestibility and body composition of L. rohita.
{"title":"Combined Effects of Citric Acid and Phytase Supplementation on Growth Performance, Nutrient Digestibility and Body Composition of Labeo rohita Fingerlings","authors":"M. Iqbal, M. Afzal, A. Yaqub, K. M. Anjum, K. Tayyab","doi":"10.4194/aquast656","DOIUrl":"https://doi.org/10.4194/aquast656","url":null,"abstract":"Aquaculturists have been concentrating their efforts to design aquafeeds using agro by-products to reduce feed costs. However, the presence of a wide variety of antinutritional factors (ANFs) in plant materials is one of the major obstacles. For this purpose, a 2x2 factorial experiment was established to evaluate the suitability of canola meal with citric acid (CA) and phytase (PHY) supplementation for growth performance, nutrient digestibility and body composition of Labeo rohita fingerlings. Five isocaloric and isonitrogenous diets; D1 (control; without CA and PHY), D2 (CA 15 g/kg+PHY 1000 FTU/kg), D3 (CA 30 g/kg+PHY 1000 FTU/kg), D4 (CA 15 g/kg+PHY 2000 FTU/kg) and D5 (CA 30 g/kg+PHY 2000 FTU/kg) were fed to fish (mean initial weight 7.61±0.31 g) in triplicates for 90-days. The growth performance was significantly increased while feed conversion ratio was decreased with D3 compared to control and other CA and PHY supplemented groups (P<0.05). Furthermore, higher digestibility (%) of nutrients (dry matter, crude protein and ash) and improved whole-body composition was also exhibited by fish fed with D3 (P<0.05). Hence, the above results elucidate that supplementation of 30 g/kg CA with 1000 FTU/kg PHY could be a useful approach for improved growth performance, nutrients digestibility and body composition of L. rohita.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41633267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
After 50 years of rapid development, today's aquaculture industry has become one of the driving forces of economic growth in many countries thanks to the increasing aquaculture production. However, less attention has been given to its growing global market demand and its contribution to relevant countries’ trade potential. Turkey has achieved significant success in aquaculture production dominated by Trout (Oncorhynchus mykiss), Sea Bass (Dicentrarchus labrax), and Sea Bream (Dicentrarchus labrax)species thanks to its geographical and biodiversity advantages. Thereby, Turkey has an exporting advantage in the face of increasing global seafood consumption demand. In this paper, we focus on the target market selection of these priority products to support the exporting potential of Turkey. Therefore, this is a multicriterion problem, and this paper aims to provide forecasting about target markets based on qualitative and quantitative criteria by combining fuzzy analytic hierarchy process (FAHP) and the technique for order preference by similarity to ideal solution (TOPSIS) methods. Seven criteria as trade balance, consumption, distance, average tariff, ease of doing business, non-tariff requirement and logistics performance index were chosen for evaluating the target markets. According to FAHP results, the trade balance criterion has the most significant effect while the distance criterion has least effect on the decision problem for ranking the target countries. According to these seven criteria, Japan is the best target market for Trout and Sea Bass while Russian Federation is the best for Sea Bream.
{"title":"Target Market Selection for the Major Aquaculture Products of Turkey - An Evaluation on Export Markets by Hybrid Multi-criteria Decision-making Approach","authors":"Pelin Çelik, Bilgen Akmermer","doi":"10.4194/aquast691","DOIUrl":"https://doi.org/10.4194/aquast691","url":null,"abstract":"After 50 years of rapid development, today's aquaculture industry has become one of the driving forces of economic growth in many countries thanks to the increasing aquaculture production. However, less attention has been given to its growing global market demand and its contribution to relevant countries’ trade potential. Turkey has achieved significant success in aquaculture production dominated by Trout (Oncorhynchus mykiss), Sea Bass (Dicentrarchus labrax), and Sea Bream (Dicentrarchus labrax)species thanks to its geographical and biodiversity advantages. Thereby, Turkey has an exporting advantage in the face of increasing global seafood consumption demand. In this paper, we focus on the target market selection of these priority products to support the exporting potential of Turkey. Therefore, this is a multicriterion problem, and this paper aims to provide forecasting about target markets based on qualitative and quantitative criteria by combining fuzzy analytic hierarchy process (FAHP) and the technique for order preference by similarity to ideal solution (TOPSIS) methods. Seven criteria as trade balance, consumption, distance, average tariff, ease of doing business, non-tariff requirement and logistics performance index were chosen for evaluating the target markets. According to FAHP results, the trade balance criterion has the most significant effect while the distance criterion has least effect on the decision problem for ranking the target countries. According to these seven criteria, Japan is the best target market for Trout and Sea Bass while Russian Federation is the best for Sea Bream.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44212616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-08DOI: 10.4194/2618-6381-v21_4_04
Dulmini Mahanama, K. Radampola, Erangi Heenkenda
A 42-day experiment was performed to evaluate the growth performance and feed utilization of Nile tilapia (Oreochromis niloticus) fingerlings fed iso-lipidic diets (5% lipid) contain 17% or 25% of cassava starch extract from fresh root (R starch) or from dry chips (D-starch) at 37% and 30% protein levels. The diets were ie: 37% protein -FM25R17 & FM25D17, 30% Protein- FM15R25 & FM15D25. 144 fish (1.11+0.02g) were distributed in 12 tanks (54 L). Protein level or starch source had no impact on final weight, length, average daily gain, specific growth rate, food conversion ratio, hepato somatic index, viscero somatic index, and Whole-body composition of fish. Protein Efficiency Ratio was not affected by starch source; however, it was significantly higher in diets with low protein content. Apparent Digestibility Coefficient of dry matter was not affected by starch source but was significantly high in low (30%) protein diet. Inclusion of R starch substantially reduced the cost of feed and increased economic profitability. Tilapia fed FM15R25 diet showed similar growth and nutrient utilization to fish fed 37% protein diets and cost of FM15R25 diet was lower than other diets. Therefore, FM15R25 is the best practical diet among the tested diets for Nile tilapia fingerlings.
{"title":"Effect of Cassava Starch Sources on Growth and Feed Utilization of Nile Tilapia Fingerlings (Oreochromis niloticus) Reared Under Two Dietary Protein Levels","authors":"Dulmini Mahanama, K. Radampola, Erangi Heenkenda","doi":"10.4194/2618-6381-v21_4_04","DOIUrl":"https://doi.org/10.4194/2618-6381-v21_4_04","url":null,"abstract":"A 42-day experiment was performed to evaluate the growth performance and feed utilization of Nile tilapia (Oreochromis niloticus) fingerlings fed iso-lipidic diets (5% lipid) contain 17% or 25% of cassava starch extract from fresh root (R starch) or from dry chips (D-starch) at 37% and 30% protein levels. The diets were ie: 37% protein -FM25R17 & FM25D17, 30% Protein- FM15R25 & FM15D25. 144 fish (1.11+0.02g) were distributed in 12 tanks (54 L). Protein level or starch source had no impact on final weight, length, average daily gain, specific growth rate, food conversion ratio, hepato somatic index, viscero somatic index, and Whole-body composition of fish. Protein Efficiency Ratio was not affected by starch source; however, it was significantly higher in diets with low protein content. Apparent Digestibility Coefficient of dry matter was not affected by starch source but was significantly high in low (30%) protein diet. Inclusion of R starch substantially reduced the cost of feed and increased economic profitability. Tilapia fed FM15R25 diet showed similar growth and nutrient utilization to fish fed 37% protein diets and cost of FM15R25 diet was lower than other diets. Therefore, FM15R25 is the best practical diet among the tested diets for Nile tilapia fingerlings.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41895610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Molecular biology developments have led to fast growth in new methods for fish disease diagnosis. Molecular diagnostic methods are rapid and more specific, more sensitive than the culture of pathogens, serology, histology, and biochemical methods which are traditionally utilized to identify causative agent fish disease. Molecular diagnostic methods are valuable for detecting specific pathogens that are difficult to culture in vitro or require a long cultivation period and it significantly more rapid in providing results compared to culture. It enables earlier informed decision-making and rapid diagnosis of bacteremia, particularly for low levels of bacteria in specimens. Molecular techniques which have the major significance are mainly PCR-based molecular diagnostic methods including Polymerase Chain Reaction (PCR), Real-Time Polymerase Chain Reaction (RT-PCR), Multiplex Polymerase Chain Reaction (multiplexPCR), and Random Amplified Polymorphic DNA (RAPD). These have been increasingly utilized to diagnose fish disease for the last recent years. Molecular diagnostic methods can detect pathogens from asymptomatic fish, so disease outbreaks could be prevented. As a consequence, antibiotic treatment can be reduced and the development of antibiotic-resistant bacteria can be eliminated. In this review paper, we attempt to summarize the potentiality of PCR-based molecular diagnostic methods and their application in fish pathogen identification.
{"title":"Potential Application of PCR Based Molecular Methods in Fish Pathogen Identification: A Review","authors":"Md. Ali Arman Ador, Md. Shameul Haque, Sulav Indra Paul, Jui Chakma, Rakib Ehsan, Ashikur Rahman","doi":"10.4194/2618-6381/aquast621","DOIUrl":"https://doi.org/10.4194/2618-6381/aquast621","url":null,"abstract":"Molecular biology developments have led to fast growth in new methods for fish disease diagnosis. Molecular diagnostic methods are rapid and more specific, more sensitive than the culture of pathogens, serology, histology, and biochemical methods which are traditionally utilized to identify causative agent fish disease. Molecular diagnostic methods are valuable for detecting specific pathogens that are difficult to culture in vitro or require a long cultivation period and it significantly more rapid in providing results compared to culture. It enables earlier informed decision-making and rapid diagnosis of bacteremia, particularly for low levels of bacteria in specimens. Molecular techniques which have the major significance are mainly PCR-based molecular diagnostic methods including Polymerase Chain Reaction (PCR), Real-Time Polymerase Chain Reaction (RT-PCR), Multiplex Polymerase Chain Reaction (multiplexPCR), and Random Amplified Polymorphic DNA (RAPD). These have been increasingly utilized to diagnose fish disease for the last recent years. Molecular diagnostic methods can detect pathogens from asymptomatic fish, so disease outbreaks could be prevented. As a consequence, antibiotic treatment can be reduced and the development of antibiotic-resistant bacteria can be eliminated. In this review paper, we attempt to summarize the potentiality of PCR-based molecular diagnostic methods and their application in fish pathogen identification.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44201100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-30DOI: 10.4194/2618-6381-v21_4_05
F. Massa, I. Aydin, Davide Fezzardi, B. Akbulut, A. Atanasoff, A. T. Beken, V. Bekh, Yuliia Buhlak, I. Burlachenko, E. Can, S. Carboni, F. Caruso, M. Dağtekin, Kostiantyn Demianenko, H. Déniz, Dilek Fidan, Linda Fourdain, M. Frederiksen, A. Guchmanidze, Housam Hamza, J. Harvey, M. Nenciu, G. Nikolov, V. Niță, Muhammed Doğan Özdemir, E. Petrova-Pavlova, G. Popescu, F. Rad, Ş. S. Can, J. Theodorou, Behnan Thomas, Nicolò Tonachella, E. Tribilustova, I. Yakhontova, A. F. Yeşilsu, Güzel Yücel-Gier
Responsible aquaculture, the farming of aquatic organisms, is a sustainable strategic sector for land and coastal communities. It significantly contributes to food security and enhancement of economic development; it provides employment opportunities and often contributes to the ecological services provided by the environment. According to the Food and Agriculture Organization of the United Nations (FAO), the contribution of aquaculture to the global food security is widely demonstrated by an astounding industry growth of 7.5% per year since 1970. In 2018, aquaculture reached the all-time highest production of 114.5 million tonnes in live weight with a total farm gate sale value of USD 263.6 billion. This makes aquaculture a key player within the Blue Growth concept and a strong contributor to some of its key Sustainable Development Goals (SDG). This is particularly true in geographical areas where dependence of local economies on fishery products is high, and yet access to sustainable landings is hampered by ecological barriers. One such area is represented by the Black Sea basin. Whilst the Black Sea annual capture fishery production has varied considerably since 1990 and its current landings are significant, growing attention is currently given to boost aquaculture development along the Black Sea bordering countries, with marine aquaculture being considered as an important contributor to the total fisheries production. Nonetheless, aquaculture development in this region is not homogenous and its development has, so far, been limited by environmental, economic, social, and more generally governance issues. This paper, for the first time, attempts to provide a comprehensive fresh outlook of the aquaculture sector in the Black Sea, stressing the importance of regional cooperation as an essential pillar to support the sustainable development of the industry. The paper addresses aquaculture in the Black Sea from different perspectives: it outlines the key characteristics of the Black Sea environment; it discusses the most common farmed aquatic species and the potential for new ones; it frames the national approaches to aquaculture development, sharing information about success stories, while shedding light on the main challenges and priorities ahead. This collective endeavour will represent a helpful contribution to Black Sea riparian countries to answer the many questions they have, and expectations they hold from the aquaculture sector.
{"title":"Black Sea Aquaculture: Legacy, Challenges & Future Opportunities","authors":"F. Massa, I. Aydin, Davide Fezzardi, B. Akbulut, A. Atanasoff, A. T. Beken, V. Bekh, Yuliia Buhlak, I. Burlachenko, E. Can, S. Carboni, F. Caruso, M. Dağtekin, Kostiantyn Demianenko, H. Déniz, Dilek Fidan, Linda Fourdain, M. Frederiksen, A. Guchmanidze, Housam Hamza, J. Harvey, M. Nenciu, G. Nikolov, V. Niță, Muhammed Doğan Özdemir, E. Petrova-Pavlova, G. Popescu, F. Rad, Ş. S. Can, J. Theodorou, Behnan Thomas, Nicolò Tonachella, E. Tribilustova, I. Yakhontova, A. F. Yeşilsu, Güzel Yücel-Gier","doi":"10.4194/2618-6381-v21_4_05","DOIUrl":"https://doi.org/10.4194/2618-6381-v21_4_05","url":null,"abstract":"Responsible aquaculture, the farming of aquatic organisms, is a sustainable strategic sector for land and coastal communities. It significantly contributes to food security and enhancement of economic development; it provides employment opportunities and often contributes to the ecological services provided by the environment. According to the Food and Agriculture Organization of the United Nations (FAO), the contribution of aquaculture to the global food security is widely demonstrated by an astounding industry growth of 7.5% per year since 1970. In 2018, aquaculture reached the all-time highest production of 114.5 million tonnes in live weight with a total farm gate sale value of USD 263.6 billion. This makes aquaculture a key player within the Blue Growth concept and a strong contributor to some of its key Sustainable Development Goals (SDG). This is particularly true in geographical areas where dependence of local economies on fishery products is high, and yet access to sustainable landings is hampered by ecological barriers. One such area is represented by the Black Sea basin. Whilst the Black Sea annual capture fishery production has varied considerably since 1990 and its current landings are significant, growing attention is currently given to boost aquaculture development along the Black Sea bordering countries, with marine aquaculture being considered as an important contributor to the total fisheries production. Nonetheless, aquaculture development in this region is not homogenous and its development has, so far, been limited by environmental, economic, social, and\u0000more generally governance issues. This paper, for the first time, attempts to provide a comprehensive fresh outlook of the aquaculture sector in the Black Sea, stressing the importance of regional cooperation as an essential pillar to support the sustainable development of the industry. The paper addresses aquaculture in the Black Sea from\u0000different perspectives: it outlines the key characteristics of the Black Sea environment; it discusses the most common farmed aquatic species and the potential for new ones; it frames the national approaches to aquaculture development, sharing information about success stories, while shedding light on the main challenges and priorities ahead. This collective endeavour will represent a helpful contribution to Black Sea riparian countries to answer the many questions they have, and expectations they hold from the aquaculture sector.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49567070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.4194/2618-6381-v21_4_03
A. Hakim, P. Wullandari, N. Zulfia, T. Widianto, B. B. Sedayu
The objective of this study was to investigate effects of Spirulina meal (SM) inclusion in fish feed formulations on their physical and protein content. Four formulations of fish feed were carried out i.e. a control diet with fishmeal (FM) as the single protein source and three others formulations containing Spirulina meal (SM) ingredient in different ratios of FM: SM (w/w) namely 75:25; 50:50 and 25:75 (w/w). All the formulations were processed into pellets using an extruder with a 600 rpm screw speed, 100 °C barrel temperature, and 3 mm diameter of dies. The dried pellets were then evaluated with respect to the protein content and physical properties i.e. expansion ratio, unit density, floatability and hardness. In general, the most optimum pellet formulation was found at the ratio of 25:75 (FM: SM) ingredients resulted in pellet with higher protein (38.89%) and expand ratio (1.65 fold), the lowest unit density (0.473 mg/mm3), the highest floatability (100%) and less hardness (32.53 N). Therefore, formulation of floating fish feed included with Spirulina are an option for improving its protein content and physical properties.
{"title":"Inclusion of Spirulina in Floating Fish Feed Production: Protein and Physical Quality","authors":"A. Hakim, P. Wullandari, N. Zulfia, T. Widianto, B. B. Sedayu","doi":"10.4194/2618-6381-v21_4_03","DOIUrl":"https://doi.org/10.4194/2618-6381-v21_4_03","url":null,"abstract":"The objective of this study was to investigate effects of Spirulina meal (SM) inclusion in fish feed formulations on their physical and protein content. Four formulations of fish feed were carried out i.e. a control diet with fishmeal (FM) as the single protein source and three others formulations containing Spirulina meal (SM) ingredient in different ratios of FM: SM (w/w) namely 75:25; 50:50 and 25:75 (w/w). All the formulations were processed into pellets using an extruder with a 600 rpm screw speed, 100 °C barrel temperature, and 3 mm diameter of dies. The dried pellets were then evaluated with respect to the protein content and physical properties i.e. expansion ratio, unit density, floatability and hardness. In general, the most optimum pellet formulation was found at the ratio of 25:75 (FM: SM) ingredients resulted in pellet with higher protein (38.89%) and expand ratio (1.65 fold), the lowest unit density (0.473 mg/mm3), the highest floatability (100%) and less hardness (32.53 N). Therefore, formulation of floating fish feed included with Spirulina are an option for improving its protein content and physical properties.","PeriodicalId":36343,"journal":{"name":"Aquaculture Studies","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49099899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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