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Point-of-care potentials of lateral flow-based field screening for MYCOPLASMA BOVIS infections: a literature review 基于侧向流的MYCOPLASMA BOVIS感染现场筛查的护理点潜力:文献综述
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-22 DOI: 10.1093/biomethods/bpae034
I. Fasogbon, Erick Nyakundi Ondari, Tusubira Deusdedit, Loganathan Rangasamy, Sasirekha Krishnan, P. M. Aja
Point-of-care (POC) field screening for tools for Mycoplasma bovis (M. bovis) is still lacking due to the requirement for a simple, robust field-applicable test that does not entail specialized laboratory equipment. In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines, this review identifies the methodologies that were retrieved based on our search strategy, that have been reported for the diagnosis of m. bovis infection between 2014 and diagnostics. A search criteria was generated to curate one hundred and three articles, which were reduced in number (to 46), following the screening guidelines of PRISMA. The 43 articles included in the study present twenty-five different assay methods. The assay methods were grouped as microbiological culture, serological assay, PCR-based assay, LAMP-based assay, NGS-based assay, or Lateral flow assay. We, however, focus our discussion on the three lateral flow-based assays relative to others, highlighting the advantages they present above the other techniques and their potential applicability as a POC diagnostic test for M. bovis infections. We therefore call for further research on developing a lateral flow-based screening tool that could revolutionize the diagnosis of M. bovis infection.
由于需要一种不需要专门实验室设备的简单、可靠的现场适用检验方法,目前仍缺乏对牛支原体(M. bovis)工具的护理点(POC)现场筛查。根据系统综述和荟萃分析首选报告项目(PRISMA)指南,本综述确定了根据我们的搜索策略检索到的方法,这些方法在 2014 年至诊断期间已被报道用于诊断牛支原体感染。根据 PRISMA 的筛选指南,我们制定了检索标准,并对 103 篇文章进行了筛选,最终将文章数量减少到 46 篇。本研究收录的 43 篇文章介绍了 25 种不同的检测方法。检测方法分为微生物培养法、血清学检测法、基于 PCR 的检测法、基于 LAMP 的检测法、基于 NGS 的检测法或侧流检测法。不过,相对于其他方法,我们重点讨论了三种基于侧向流的检测方法,强调了它们优于其他技术的优势及其作为牛海绵状芽孢杆菌感染 POC 诊断测试的潜在适用性。因此,我们呼吁进一步研究开发基于侧向流的筛查工具,以彻底改变牛海绵状芽孢杆菌感染的诊断方法。
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引用次数: 0
Two methods of isolation of rat aortic smooth muscle cells with high yield 两种高产率分离大鼠主动脉平滑肌细胞的方法
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-22 DOI: 10.1093/biomethods/bpae038
Saran Lotfollahzadeh, Asha Jose, Esha Shafiq zarnaab, Nourhan El Sherif, Michael Smith, Jingyan Han, Francesca Seta, V. Chitalia
Vascular smooth muscle cells (VSMCs) are an integral part of blood vessels and are the focus of intensive research in vascular biology, translational research, and cardiovascular diseases. Though immortalized vascular smooth muscle cell lines are available, their use is limited, underscoring the need for primary VSMCs. There are several methods for isolating primary cells from mice. However, the isolation method from rat blood vessels requires optimization, given the differences in the aorta of mice and rats. Here we compare two methods for VSMCs isolation from rats: enzymatic digestion and the “block” method. We observed a significantly higher yield of VSMCs using the enzymatic digestion method. We further confirmed that VSMCs expressed well-established VSMC-specific markers (calponin) with both methods and observed the persistence of this marker up to 9 passages, suggesting a continuation of the secretory phenotype of VSMCs. Overall, this work compares two methods and demonstrates a practical and effective method for isolating VSMCs from rat aorta, providing vascular biologists with a valuable and reliable experimental tool.
血管平滑肌细胞(VSMC)是血管不可或缺的组成部分,也是血管生物学、转化研究和心血管疾病领域深入研究的重点。虽然有永生化的血管平滑肌细胞系,但其用途有限,因此需要原代血管平滑肌细胞。从小鼠体内分离原代细胞有多种方法。然而,由于小鼠和大鼠的主动脉不同,从大鼠血管中分离的方法需要优化。在此,我们比较了从大鼠体内分离 VSMC 的两种方法:酶解法和 "块状 "法。我们观察到酶解法的 VSMC 得率明显更高。我们还进一步证实,这两种方法都能使 VSMC 表达成熟的 VSMC 特异性标记(钙蛋白),并观察到这一标记可持续到 9 次传代,这表明 VSMC 的分泌表型仍在继续。总之,这项工作对两种方法进行了比较,展示了一种从大鼠主动脉分离 VSMC 的实用有效方法,为血管生物学家提供了一种宝贵可靠的实验工具。
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引用次数: 0
Isolation of short RNAs with homogenous 3’-ends using quaternary-amine anion exchange chromatography 利用季胺阴离子交换色谱法分离具有同源 3'- 末端的短 RNA
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-17 DOI: 10.1093/biomethods/bpae033
Zixian Li, Mia Bilic, Bhushan Nagar
Visualizing RNA-protein interactions through structural approaches requires the use of RNA molecules purified to homogeneity. We describe here a simple and effective method, free of acrylamide contamination and without using UV radiation, to separate in vitro synthesized, heterogeneous RNA transcripts (up to ∼15 nucleotides) at single nucleotide resolution by quaternary-amine anion exchange chromatography. The quality of short RNAs isolated through this method is validated by gel electrophoresis, mass spectrometry, and crystallization with a protein binding partner.
通过结构方法观察 RNA 蛋白相互作用需要使用纯化到均一的 RNA 分子。我们在此介绍一种简单有效的方法,该方法无丙烯酰胺污染,无需紫外线辐射,可通过季胺阴离子交换色谱法以单核苷酸分辨率分离体外合成的异质 RNA 转录本(最多 15 个核苷酸)。通过凝胶电泳、质谱分析和与蛋白质结合的结晶验证了用这种方法分离出的短 RNA 的质量。
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引用次数: 0
High throughput Surface Epitope Immunoaffinity Isolation of Extracellular Vesicles and Downstream Analysis 细胞外囊泡的高通量表面表位免疫亲和分离及下游分析
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-17 DOI: 10.1093/biomethods/bpae032
Ramin Khanabdali, Michelle Mandrekar, Rick Grygiel, Phuoc-an Vo, Carlos Palma, Sara Nikseresht, Siena Barton, Mozhgan Shojaee, Sadman Bhuiyan, Kartini Asari, Susan Belzer, Khairul Ansari, Jermaine I Coward, Lewis Perrin, John Hooper, Dominic Guanzon, Andrew Lai, Carlos Salomon, Kevin Kershner, Christine Newton, Douglas Horejsh, Gregory E. Rice
Extracellular vesicles (EVs), including exosomes, have significant potential for diagnostic and therapeutic applications. The lack of standardized methods for efficient and high throughput isolation and analysis of EVs, however, has limited their widespread use in clinical practice. Surface epitope immunoaffinity (SEI) isolation utilises affinity ligands, including antibodies, aptamers, or lectins, that target specific surface proteins present on EVs. Paramagnetic bead-SEI isolation represents a fit-for-purpose solution for the reproducible, high throughput isolation of EVs from biofluids and downstream analysis of RNA, protein and lipid biomarkers that is compatible with clinical laboratory workflows. This study evaluates a new surface epitope immunoaffinity isolation method for enriching subpopulations of EVs. EVs were isolated from human plasma using a bead-based SEI method designed for on-bead and downstream analysis of EV-associated RNA and protein biomarkers. Western blot analysis confirmed the presence of EV markers in the captured nanoparticles. Mass spectrometry analysis of the SEI lysate identified over 1500 proteins, with the top 100 including known EV-associated proteins. miRNA sequencing followed by RT-qPCR analysis identified EV-associated miRNA transcripts. Using SEI, EVs were isolated using automated high throughput particle moving instruments, demonstrating equal or higher protein and miRNA yield and recovery compared to manual processing. SEI is a rapid, efficient, and high throughput method for isolating enriched populations of EVs; effectively reducing contamination and enabling the isolation of a specific subpopulation of EVs. In this study, high throughput EV isolation and RNA extraction have been successfully implemented. This technology holds great promise for advancing the field of EV research and facilitating their application for biomarker discovery and clinical research.
包括外泌体在内的细胞外囊泡(EVs)在诊断和治疗方面具有巨大的应用潜力。然而,由于缺乏高效、高通量分离和分析 EVs 的标准化方法,限制了它们在临床实践中的广泛应用。表面表位免疫亲和(SEI)分离利用的是亲和配体,包括抗体、适配体或凝集素,它们针对的是存在于 EVs 上的特定表面蛋白。顺磁珠-SEI 分离是一种适用于从生物流体中可重复、高通量分离 EVs 并进行下游 RNA、蛋白质和脂质生物标记物分析的解决方案,与临床实验室工作流程兼容。本研究评估了一种用于富集 EVs 亚群的新型表面表位免疫亲和分离方法。使用一种基于珠子的 SEI 方法从人血浆中分离出了 EVs,该方法设计用于珠子上和下游的 EV 相关 RNA 和蛋白质生物标记物分析。Western 印迹分析证实了捕获的纳米颗粒中存在 EV 标记。SEI 裂解液的质谱分析鉴定了超过 1500 种蛋白质,其中前 100 种包括已知的 EV 相关蛋白质。利用 SEI,使用自动高通量粒子移动仪器分离了 EV,与人工处理相比,蛋白质和 miRNA 的产量和回收率相同或更高。SEI 是一种快速、高效、高通量的方法,可用于分离富集的 EVs 群体;有效减少污染,并能分离特定的 EVs 亚群。本研究成功实现了高通量 EV 分离和 RNA 提取。这项技术有望推动 EV 研究领域的发展,并促进其在生物标记物发现和临床研究中的应用。
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引用次数: 0
Meaning and prediction of "excess mortality": A comparison of Covid- and pre-Covid mortality data in 31 Eurostat countries from 1965 to 2021 超额死亡率 "的含义和预测:1965年至2021年欧盟统计局31个国家Covid死亡率数据与Covid前死亡率数据的比较
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-17 DOI: 10.1093/biomethods/bpae031
Bernhard Gill, Theresa Kehler, Michael Schneider
Determining "excess mortality" makes it possible to compare the burden of disasters between countries and over time, and thus also to evaluate the success of mitigation measures. However, the debate on Covid-19 has exposed that calculations of excess mortalities vary considerably depending on the method and its specification. Moreover, it is often unclear what exactly is meant by "excess mortality". We define excess mortality as the excess over the number of deaths that would have been expected counter-factually, ie without the catastrophic event in question. Based on this definition, we use a very parsimonious calculation method, namely the linear extrapolation of death figures from previous years to determine the excess mortality during the Covid-19 pandemic. But unlike most other literature on this topic, we first evaluated and optimised the specification of our method using a larger historical data set in order to identify and minimise estimation errors and biases. The result shows that excess mortality rates in the literature are often inflated. Moreover, they would have exhibited considerable excess mortalities in the period before Covid-19, if this value had already been of public interest at that time. Three conclusions can be drawn from this study and its findings: 1) All calculation methods for current figures should first be evaluated against past figures. 2) To avoid alarm fatigue, for mass media and policy communication thresholds should be introduced which would differentiate between "usual fluctuations" and "remarkable excess". 3) Statistical offices could provide more realistic estimates.
确定 "超额死亡率 "可以比较不同国家和不同时期的灾害负担,从而评估减灾措施是否成功。然而,关于 Covid-19 的讨论表明,超额死亡率的计算方法和具体说明大相径庭。此外,"超额死亡率 "的确切含义通常也不明确。我们将超额死亡率定义为超出反事实预期死亡人数的部分,即没有发生相关灾难事件时的预期死亡人数。根据这一定义,我们采用了一种非常简便的计算方法,即通过对前几年的死亡数字进行线性外推来确定 Covid-19 大流行期间的超额死亡率。但与其他大多数相关文献不同的是,我们首先利用一个更大的历史数据集对我们的方法进行了评估和优化,以确定并尽量减少估计误差和偏差。结果表明,文献中的超额死亡率往往被夸大了。此外,如果这一数值当时已经引起公众的关注,那么在科维德-19 之前的时期,它们就会表现出相当高的超额死亡率。从这项研究及其结果中可以得出三个结论:1) 当前数据的所有计算方法都应首先对照过去的数据进行评估。2) 为避免 "警报疲劳",在大众传媒和政策沟通中应引入阈值,以区分 "通常的波动" 和 "显著的过度"。3) 统计部门可以提供更切合实际的估计数字。
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引用次数: 0
Comparing skin swabs, buccal swabs, and toe clips for amphibian genetic sampling, a case study with a small anuran (acris blanchardi) 两栖动物基因采样中皮肤拭子、口腔拭子和脚趾夹的比较,以小型无尾目动物(acris blanchardi)为例进行研究
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-16 DOI: 10.1093/biomethods/bpae030
Travis A Rainey, Emily E Tryc, Kirsten E Nicholson
Multiple methods for collecting genetic samples from amphibians exist, each with their own implications for study design, animal welfare, and costs. Toe clipping is one common method, but there is ongoing debate regarding its potential detriment. Less invasive methods should be implemented, if efficacious, as amphibians are a particularly vulnerable vertebrate group. Skin and buccal swabbing are less invasive methods for genetic sampling, but the potential for contamination and a lower yield of DNA may exist. To compare these methods, we gathered skin swabs, buccal swabs, and toe clips from the same individuals of a relatively small anuran species, Blanchard’s Cricket Frog (Acris blanchardi). We then compared DNA yield, DNA purity, amplification success rate, and genotypic data quality among sample types. We found toe clips and buccal swabs generated similar DNA yield and purity, with skin swabs yielding significantly less DNA of significantly lower purity than the other sample types. Amplification success rate was significantly higher using toe clips compared to the other sample types, though buccal swab samples amplified more readily than skin swabs. Genotypic data from toe clips and buccal swabs did not differ significantly in quality, but skin swab data quality was significantly lowest among sample types. Thus, skin swabbing could produce erroneous data in some situations, but buccal swabbing is likely an effective substitute to toe clipping, even for small species. Our results can help future researchers select which genetic sampling method might best suit their research needs.
从两栖动物身上采集基因样本有多种方法,每种方法对研究设计、动物福利和成本都有各自的影响。剪趾是一种常见的方法,但关于这种方法的潜在危害一直存在争议。由于两栖动物是特别脆弱的脊椎动物群体,因此如果有效,应采用侵入性较小的方法。皮肤和颊拭子是侵入性较小的基因采样方法,但可能存在污染和 DNA 产量较低的可能性。为了比较这些方法,我们收集了皮肤拭子、口腔拭子和脚趾夹,这些拭子取自相对较小的无脊椎动物物种--布兰查德蟋蟀蛙(Acris blanchardi)的相同个体。然后,我们比较了不同样本类型的 DNA 产量、DNA 纯度、扩增成功率和基因型数据质量。我们发现脚趾夹和颊拭子产生的DNA产量和纯度相似,而皮肤拭子产生的DNA产量和纯度明显低于其他类型的样本。与其他样本类型相比,脚趾夹的扩增成功率明显更高,但颊拭子样本比皮肤拭子更容易扩增。趾夹和口腔拭子的基因型数据在质量上没有明显差异,但皮肤拭子的数据质量在所有样本类型中明显最低。因此,在某些情况下,皮肤拭子可能会产生错误的数据,但口腔拭子可能是剪趾的有效替代品,即使对于小型物种也是如此。我们的研究结果可以帮助未来的研究人员选择最适合其研究需要的基因采样方法。
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引用次数: 0
A highly sensitive stem-loop RT-qPCR method to study siRNA intracellular pharmacokinetics and pharmacodynamics 研究 siRNA 细胞内药代动力学和药效学的高灵敏度茎环 RT-qPCR 方法
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-06 DOI: 10.1093/biomethods/bpae029
Lin Chen, Caroline Bosmajian, Sukyung Woo
Small interfering RNA (siRNA) is a powerful tool for sequence-specific silencing of disease-related genes. In this study, we established and validated a stem-loop reverse transcription real-time polymerase chain reaction (RT-qPCR) method applicable for both chemically unmodified and modified siRNA, aiming to elucidate mechanistic intracellular pharmacokinetic and pharmacodynamic (PK/PD) properties of siRNA. We conducted a comprehensive evaluation of factors affecting intracellular siRNA quantification. Our study revealed that immobilization-based siRNA extraction introduced high variation, making it unsuitable for absolute quantification. Conversely, direct cell lysis followed by stem-loop RT-qPCR demonstrated excellent reproducibility, with a quantification range from 0.0002 to 20 femtomole (fmole) for unmodified siRNA and 0.02 to 20 fmole for modified siRNA. The design of a 6-basepair overlapping RT primer facilitated the distinction of full-length antisense from its 3’ metabolites, and pre-annealing of antisense to RT primer enhanced sensitivity and reproducibility. Differences in siRNA loss during storage and sample processing were noted among microcentrifuge tubes from various manufacturers. Endogenous miR-16 served as a reference for normalizing cytoplasmic siRNA, while protein concentration post-immunoprecipitation lysis was used to normalize RISC-loaded siRNA levels. This method successfully enabled a detailed characterization of the time profiles of cytoplasmic and RISC-loaded siRNA, advancing of the in vitro-in vivo translation of siRNA therapeutics.
小干扰 RNA(siRNA)是序列特异性沉默疾病相关基因的有力工具。在这项研究中,我们建立并验证了一种适用于化学未修饰和修饰 siRNA 的干环反向转录实时聚合酶链反应(RT-qPCR)方法,旨在阐明 siRNA 的细胞内药代动力学和药效学(PK/PD)机理特性。我们对影响细胞内 siRNA 定量的因素进行了全面评估。我们的研究发现,基于固定化技术的 siRNA 提取会带来很大的变化,因此不适合绝对定量。相反,直接裂解细胞后进行干环 RT-qPCR 则表现出极佳的重现性,未修饰 siRNA 的定量范围为 0.0002 至 20 飞摩尔(fmole),修饰 siRNA 的定量范围为 0.02 至 20 飞摩尔。6 碱基对重叠 RT 引物的设计有助于区分全长反义和其 3' 代谢产物,反义与 RT 引物的预退火提高了灵敏度和重现性。不同厂家生产的微离心管在储存和样品处理过程中 siRNA 的损失存在差异。内源性 miR-16 可作为细胞质 siRNA 正常化的参考,而免疫沉淀裂解后的蛋白质浓度可用于 RISC 加载的 siRNA 水平的正常化。这种方法成功地详细描述了细胞质和 RISC 负载 siRNA 的时间曲线,推进了 siRNA 疗法的体外-体内转化。
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引用次数: 0
Multiparameter flow cytometric detection and analysis of rare cells in in vivo models of cancer metastasis 多参数流式细胞仪检测和分析体内癌症转移模型中的稀有细胞
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-27 DOI: 10.1093/biomethods/bpae026
Mikaela M. Mallin, Louis T A Rolle, Kenneth J Pienta, S. Amend
Rapid and reliable circulating tumor cell (CTC) and disseminated tumor cell (DTC) detection is critical for rigorous evaluation of in vivo metastasis models. Clinical data shows that each step of the metastatic cascade presents increasing barriers to success, limiting the number of successful metastatic cells to fewer than 1 in 1,500,000,000. As such, it is critical for scientists to employ approaches that allow for evaluation of metastatic competency at each step of the cascade. Here, we present a flow cytometry-based method that enables swift and simultaneous comparison of both CTCs and DTCs from single animals, enabling evaluation of multiple metastatic steps within a single model system. We present the necessary gating strategy and optimized sample preparation conditions necessary to capture CTCs and DTCs using this approach. We also provide proof-of-concept experiments emphasizing the appropriate limits of detection of these conditions. Most importantly, we successfully recover CTCs and DTCs from murine blood and bone marrow. In supplemental materials, we expand the applicability of our method to lung tissue and exemplify a potential multi-plexing strategy to further characterize recovered CTCs and DTCs. This approach to multiparameter flow cytometric detection and analysis of rare cells in in vivo models of metastasis is reproducible, high-throughput, broadly applicable and highly adaptable to a wide range of scientific inquiries. Most notably, it simplifies the recovery and analysis of CTCs and DTCs from the same animal, allowing for a rapid first look at the comparative metastatic competency of various model systems throughout multiple steps of the metastatic cascade.
快速可靠的循环肿瘤细胞(CTC)和播散肿瘤细胞(DTC)检测对于严格评估体内转移模型至关重要。临床数据显示,转移级联的每一步都会对成功造成越来越大的障碍,成功转移的细胞数量限制在 1,500,000,000 中的不到 1 个。因此,对于科学家来说,采用能够评估级联过程中每一步转移能力的方法至关重要。在这里,我们介绍了一种基于流式细胞术的方法,该方法能同时快速比较单只动物的 CTC 和 DTC,从而在单一模型系统中评估多个转移步骤。我们介绍了使用这种方法捕获 CTC 和 DTC 所需的选通策略和优化的样本制备条件。我们还提供了概念验证实验,强调了这些条件的适当检测限。最重要的是,我们成功地从小鼠血液和骨髓中回收了 CTC 和 DTC。在补充材料中,我们将方法的适用范围扩大到了肺组织,并举例说明了一种潜在的多重流式细胞分析策略,以进一步确定回收的 CTC 和 DTC 的特征。这种在体内转移模型中对稀有细胞进行多参数流式细胞仪检测和分析的方法具有可重复性、高通量性、广泛适用性和高度适应性,可用于各种科学研究。最值得注意的是,它简化了来自同一动物的 CTCs 和 DTCs 的回收和分析,可快速初步了解各种模型系统在转移级联的多个步骤中的比较转移能力。
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引用次数: 0
Macro-based collagen quantification and segmentation in picrosirius red-stained heart sections using light microscopy. 使用光学显微镜对皮色红染色的心脏切片进行基于宏观的胶原蛋白定量和分割。
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-27 eCollection Date: 2024-01-01 DOI: 10.1093/biomethods/bpae027
Julian Kammerer, Alexandra Cirnu, Tatjana Williams, Melanie Hasselmeier, Mike Nörpel, Ruping Chen, Brenda Gerull

Picrosirius red staining constitutes an important and broadly used tool to visualize collagen and fibrosis in various tissues. Although multiple qualitative and quantitative analysis methods to evaluate fibrosis are available, many require specialized devices and software or lack objectivity and scalability. Here, we aimed to develop a versatile and powerful "QuantSeg" macro in the FIJI image processing software capable of automated, robust, and quick collagen quantification in cardiac tissue from light micrographs. To examine different patterns of fibrosis, an optional segmentation algorithm was implemented. To ensure the method's validity, we quantified the collagen content in a set of wild-type versus plakoglobin-knockout murine hearts exhibiting extensive fibrosis using both the macro and an established, fluorescence microscopy-based method, and compared results. To demonstrate the capabilities of the segmentation feature, rat hearts were examined post-myocardial infarction. We found the QuantSeg macro to robustly detect the differences in fibrosis between knockout and control hearts. In sections with low collagen content, the macro yielded more consistent results than using the fluorescence microscopy-based technique. With its wide range of output parameters, ease of use, cost effectiveness, and objectivity, the QuantSeg macro has the potential to become an established method for analysis of PSR-stained tissue. The novel segmentation feature allows for automated evaluation of different patterns of cardiac fibrosis for the first time.

毕赤染色是观察各种组织中胶原蛋白和纤维化的一种重要且广泛使用的工具。虽然有多种定性和定量分析方法可用于评估纤维化,但许多方法都需要专门的设备和软件,或者缺乏客观性和可扩展性。在这里,我们的目标是在 FIJI 图像处理软件中开发一个多功能且功能强大的 "QuantSeg "宏,该宏能够自动、稳健、快速地从光显微照片中量化心脏组织中的胶原蛋白。为了检查不同的纤维化模式,我们采用了一种可选的分割算法。为确保该方法的有效性,我们使用宏法和一种成熟的基于荧光显微镜的方法量化了一组表现出广泛纤维化的野生型和plakoglobin-knockout小鼠心脏中的胶原蛋白含量,并对结果进行了比较。为了证明分割功能的能力,我们对心肌梗塞后的大鼠心脏进行了检查。我们发现,QuantSeg 宏能稳健地检测出基因敲除心脏和对照心脏纤维化的差异。在胶原蛋白含量较低的切片中,宏得到的结果比使用基于荧光显微镜的技术更一致。QuantSeg 宏有广泛的输出参数、易用性、成本效益和客观性,有望成为分析 PSR 染色组织的成熟方法。新颖的分割功能首次实现了对心脏纤维化不同模式的自动评估。
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引用次数: 0
Correction to: An improved method for measuring catalase activity in biological samples. 更正:测量生物样本中过氧化氢酶活性的改进方法。
IF 3.6 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-26 eCollection Date: 2024-01-01 DOI: 10.1093/biomethods/bpae025

[This corrects the article DOI: 10.1093/biomethods/bpae015.].

[此处更正了文章 DOI:10.1093/biomethods/bpae015]。
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引用次数: 0
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