The ability to respond to changing consumer and industrial wastes is critical for water treatment. Surfactants are of importance because of their increasing prevalence in home and industrial settings, as well as the challenges they provide to standard treatment. Surface active agents (SAAs) are chemical compounds that are discharged into the environment. They may have a deleterious impact on ecosystem biotic components. They also produce an increase in the aqueous phase solubility of organic contaminants. The destiny of SAAs in the environment is currently uncertain. Recognizing this issue will help to safeguard live species and maintain the quality and balance of a diverse ecosystem.This critical evaluation considers studies from several technical disciplines to offer an up-to-date summary of the environmental effects of surfactants. A variety of technologies are discussed, as well as their drawbacks and potential solutions.
{"title":"A Review on the Degradation of Ionic and Non Ionic Surfactants in Water","authors":"Dhiraj Dutta, Nisha Gaur, Prabir Barman, Debottam Ghosh, Rama Dubey, S. Dwivedi","doi":"10.14429/dlsj.7.17309","DOIUrl":"https://doi.org/10.14429/dlsj.7.17309","url":null,"abstract":"The ability to respond to changing consumer and industrial wastes is critical for water treatment. Surfactants are of importance because of their increasing prevalence in home and industrial settings, as well as the challenges they provide to standard treatment. Surface active agents (SAAs) are chemical compounds that are discharged into the environment. They may have a deleterious impact on ecosystem biotic components. They also produce an increase in the aqueous phase solubility of organic contaminants. The destiny of SAAs in the environment is currently uncertain. Recognizing this issue will help to safeguard live species and maintain the quality and balance of a diverse ecosystem.This critical evaluation considers studies from several technical disciplines to offer an up-to-date summary of the environmental effects of surfactants. A variety of technologies are discussed, as well as their drawbacks and potential solutions.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46196484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Podophyllum species, the source of important secondary metabolite, podophyllotoxin, is over-exploited for production of anti-cancer drugs endangering this genus globally. Lack of complete knowledge on podophyllotoxin biosynthesis is a major drawback in its cultivation and identification for alternative plants. The current study on secoisolariciresinol dehydrogenase, dirigent protein oxidase and pluviatolide synthase identifies their role in regulating podophyllotoxin biosynthesis. The present computational analysis of podophyllotoxin proposes a correlating interconnected network of pathways for podophyllotoxin biosynthesis besides identifying potential substitute species for the biosynthesis of podophyllotoxin and accounting for possible reason for variation in podophyllotoxin yield from different species of this genus
{"title":"Computational Analysis for Regulation of Podophyllotoxin Biosynthesis Pathway in Podophyllum with Potential Substitute Species","authors":"U. Srivastava, H. Sood","doi":"10.14429/dlsj.7.17445","DOIUrl":"https://doi.org/10.14429/dlsj.7.17445","url":null,"abstract":" Podophyllum species, the source of important secondary metabolite, podophyllotoxin, is over-exploited for production of anti-cancer drugs endangering this genus globally. Lack of complete knowledge on podophyllotoxin biosynthesis is a major drawback in its cultivation and identification for alternative plants. The current study on secoisolariciresinol dehydrogenase, dirigent protein oxidase and pluviatolide synthase identifies their role in regulating podophyllotoxin biosynthesis. The present computational analysis of podophyllotoxin proposes a correlating interconnected network of pathways for podophyllotoxin biosynthesis besides identifying potential substitute species for the biosynthesis of podophyllotoxin and accounting for possible reason for variation in podophyllotoxin yield from different species of this genus","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41722110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashish Bali, P. Pal, Aditya Bali, Safna Ameen, Rimsha Ahmed, M. Khan
The COVID-19 outbreak has caused an impervious financial and psychological burden. Health care professionals, including oral health care workers, have been risking fighting the pandemic. The chief objective of the current study was to estimate the rates of prevalence of depression, stress, and anxiety among the oral health care professionals in Jammu and Udaipur city. The study was delineated as an online cross-sectional questionnaire-based research. It was mailed to different practitioners between May and July 2020, particularly those offered their services in COVID centers. The participants were to fill the self report questionnaires. Then, the parameters were measured using depression, anxiety, and stress scale 21(DASS 21) and Hamilton anxiety rating scale (HARS) to measure the degrees of depression, stress, and fear among the volunteers. The target population was divided into age groups between 23 to 28 years and over 28 years. Four hundred ninety responses were received and were considered for the study. The acquired data were analysed using IBM SPSSsoftware (windows version 23). The mean and standard deviations were calculated for stress, anxiety, depression using mentioned scale. The results were compared based on gender and age group. A statistically significant variance in stress level was found between male and female groups (p=0.002) and for the two age groups (p=0.001). Using the Hamilton anxiety rating scale, no statistically significant divergence could be seen among male and female participants. The current study showed stress, anxiety, and depressions were prevalent among health care workers working in COVID pandemic situations. Therefore, mental health status must be addressed, and issues must be resolved.
{"title":"Stress Anxiety and Depression Levels Associated with COVID 19 among the Oral Health Care Workers in North India A Cross Sectional Studyare worker in North India A cross sectional study","authors":"Ashish Bali, P. Pal, Aditya Bali, Safna Ameen, Rimsha Ahmed, M. Khan","doi":"10.14429/dlsj.7.16850","DOIUrl":"https://doi.org/10.14429/dlsj.7.16850","url":null,"abstract":"The COVID-19 outbreak has caused an impervious financial and psychological burden. Health care professionals, including oral health care workers, have been risking fighting the pandemic. The chief objective of the current study was to estimate the rates of prevalence of depression, stress, and anxiety among the oral health care professionals in Jammu and Udaipur city. The study was delineated as an online cross-sectional questionnaire-based research. It was mailed to different practitioners between May and July 2020, particularly those offered their services in COVID centers. The participants were to fill the self report questionnaires. Then, the parameters were measured using depression, anxiety, and stress scale 21(DASS 21) and Hamilton anxiety rating scale (HARS) to measure the degrees of depression, stress, and fear among the volunteers. The target population was divided into age groups between 23 to 28 years and over 28 years. Four hundred ninety responses were received and were considered for the study. The acquired data were analysed using IBM SPSSsoftware (windows version 23). The mean and standard deviations were calculated for stress, anxiety, depression using mentioned scale. The results were compared based on gender and age group. A statistically significant variance in stress level was found between male and female groups (p=0.002) and for the two age groups (p=0.001). Using the Hamilton anxiety rating scale, no statistically significant divergence could be seen among male and female participants. The current study showed stress, anxiety, and depressions were prevalent among health care workers working in COVID pandemic situations. Therefore, mental health status must be addressed, and issues must be resolved.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45925064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. B. Khambholja, Dharmishtha Gamit, H. Patel, Prashant Kumar
The current study was designed to isolate and characterize urease-producing bacteria and to assess their ability to precipitate calcium carbonate. Total eight bacteria were isolated from dung-rich soil samples collected from Dakor, Gujarat. Out of these, two bacterial strains designated as DGDK-3 and DGDK-4 were found to produce a considerable level of urease in the initial screening on the urea agar medium. Based on morphological and physiological tests and more specifically by 16S rRNA gene sequencing analysis, these bacteria were identified as Quasibacillus sp. Strain DGDK-3 and Bacillus sp. Strain DGDK-4. The strains DGDK-3 and DGDK-4 showed 25 IU/ml and 89 IU/ml urease activity, respectively. Also, the efficacy of both strains was tested for calcium carbonate precipitation. Results showed that both the isolates were competent to precipitate a significant level of calcium carbonate. The current work demonstrated that urease-producing bacteria can be utilised in bio-cementationas a crack sealing agent and as a natural stabilizing agent.
{"title":"Isolation and Characterisation of Urease Producing Bacteria and their Capacity to Precipitate Calcium Carbonate","authors":"D. B. Khambholja, Dharmishtha Gamit, H. Patel, Prashant Kumar","doi":"10.14429/dlsj.7.17279","DOIUrl":"https://doi.org/10.14429/dlsj.7.17279","url":null,"abstract":"The current study was designed to isolate and characterize urease-producing bacteria and to assess their ability to precipitate calcium carbonate. Total eight bacteria were isolated from dung-rich soil samples collected from Dakor, Gujarat. Out of these, two bacterial strains designated as DGDK-3 and DGDK-4 were found to produce a considerable level of urease in the initial screening on the urea agar medium. Based on morphological and physiological tests and more specifically by 16S rRNA gene sequencing analysis, these bacteria were identified as Quasibacillus sp. Strain DGDK-3 and Bacillus sp. Strain DGDK-4. The strains DGDK-3 and DGDK-4 showed 25 IU/ml and 89 IU/ml urease activity, respectively. Also, the efficacy of both strains was tested for calcium carbonate precipitation. Results showed that both the isolates were competent to precipitate a significant level of calcium carbonate. The current work demonstrated that urease-producing bacteria can be utilised in bio-cementationas a crack sealing agent and as a natural stabilizing agent.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41461662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suraj Bharati, Himanshi Dhyani, V. Thakare, Virendra Vikram Singh, Damyanti Meher, M. Boopathi
This article reviews a detailed and timely evolution of Nuclear Biological Chemical (NBC) protective clothing. The increased threat of NBC weapons underlines the need for NBC protective clothing for first responders, service personnel as well as civilians. The material selection criteria for protective clothing have been changed considerably with the development of advance technical fibers to sustain the threat of CWA and fulfill precisely need for the NBC suits. Because of advancement, in the use and delivery of Chemical Warfare Agent (CWA), the evolution of the NBC suit is presented. The chemical protection of NBC protective clothing needs to be precisely evaluated and thereby related standard test methods are also duly accommodated. This review documented the journey of the NBC suit right from charcoal based to activated carbon spheres (ACS), and recent developments on the materials aspects of various protective clothing.
{"title":"Technology Trends and Future Opportunities in Development of NBC Protective Clothing","authors":"Suraj Bharati, Himanshi Dhyani, V. Thakare, Virendra Vikram Singh, Damyanti Meher, M. Boopathi","doi":"10.14429/dlsj.7.17199","DOIUrl":"https://doi.org/10.14429/dlsj.7.17199","url":null,"abstract":"This article reviews a detailed and timely evolution of Nuclear Biological Chemical (NBC) protective clothing. The increased threat of NBC weapons underlines the need for NBC protective clothing for first responders, service personnel as well as civilians. The material selection criteria for protective clothing have been changed considerably with the development of advance technical fibers to sustain the threat of CWA and fulfill precisely need for the NBC suits. Because of advancement, in the use and delivery of Chemical Warfare Agent (CWA), the evolution of the NBC suit is presented. The chemical protection of NBC protective clothing needs to be precisely evaluated and thereby related standard test methods are also duly accommodated. This review documented the journey of the NBC suit right from charcoal based to activated carbon spheres (ACS), and recent developments on the materials aspects of various protective clothing.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47518957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Jha, Dr. Sonia Johri, S. Shrivastava, Poonam Gupta, Kamini Yadav
Watermelon (Citrullus lanatus) is a cheap and easily available fruit in the local markets of India. The rind, which is the outer layer of watermelon, is completely edible. It is the only fruit with 90 per cent of water, and is fully edible including its rind and seeds as they contains different types of nutrients which are needed by our body in day to day life. The benefits in our body, includes reduced blood pressure, presence of different types of vitamins, such as vitamin A, B & C, as well as different types of minerals required by our body. The present study aims in evaluating the presence of different secondary metabolites in the watermelon rind. The therapeutic efficacy of watermelon rind against acrylamide toxicity in the lymphocyte cell line is studied. As selenium is an important micronutrient, an attempt has been made to prepare the selenium nanoparticles followed by its characterisation.
{"title":"Therapeutic Approach and Characterisation of Watermelon (Citrullus lanatus) Rind against Acrylamide Toxicity","authors":"N. Jha, Dr. Sonia Johri, S. Shrivastava, Poonam Gupta, Kamini Yadav","doi":"10.14429/dlsj.7.17413","DOIUrl":"https://doi.org/10.14429/dlsj.7.17413","url":null,"abstract":"Watermelon (Citrullus lanatus) is a cheap and easily available fruit in the local markets of India. The rind, which is the outer layer of watermelon, is completely edible. It is the only fruit with 90 per cent of water, and is fully edible including its rind and seeds as they contains different types of nutrients which are needed by our body in day to day life. The benefits in our body, includes reduced blood pressure, presence of different types of vitamins, such as vitamin A, B & C, as well as different types of minerals required by our body. The present study aims in evaluating the presence of different secondary metabolites in the watermelon rind. The therapeutic efficacy of watermelon rind against acrylamide toxicity in the lymphocyte cell line is studied. As selenium is an important micronutrient, an attempt has been made to prepare the selenium nanoparticles followed by its characterisation.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45089349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shalini Tailor, K. Jain, A. Marwal, M. Meena, K. Anbarasu, R. Gaur
Technological advances are getting monitored with time, and science suggests nanotechnology as the emerging future. This even holds correct with human food consumption for health benefits, where organic farming is a better solution for the rising population and is even supported by major countries instead of using chemical fertilisers and pesticides. Nanotechnology provides a platform where nanoparticles help in better management for organic farming by using it as nano fertilisers, nanocides, nano biosensors, nano growth promoters, etc. These nanomaterials can be synthesised by three different mechanisms namely; chemical, physical, and biological methods. Since the chemical and physical mode of synthesis does not follow the criteria of organic farming and have their drawbacks. Hence, the biological method, also known as the green synthesis of nanomaterials fulfills the requirement of organic farming and has achieved the attention of researchers. Extracts of plant parts (stems, roots, leaves, flowers and, fruits) and different microbes, including bacteria, fungus, and mycorrhiza can be used as a base material for the synthesis of nanoparticles under green synthesis mode. The vision behind the green synthesis of nanoparticles was to curb the hazardous effects of chemically synthesised nanoparticles. In the present review, green synthesis of major elements of organic farming namely; nano fertilisers, nano-pesticides, and nano growth promoters, their modes of transportation, their advantages, and disadvantages in organic farming are discussed.
{"title":"Outlooks of Nanotechnology in Organic Farming Management","authors":"Shalini Tailor, K. Jain, A. Marwal, M. Meena, K. Anbarasu, R. Gaur","doi":"10.14429/dlsj.7.16763","DOIUrl":"https://doi.org/10.14429/dlsj.7.16763","url":null,"abstract":"Technological advances are getting monitored with time, and science suggests nanotechnology as the emerging future. This even holds correct with human food consumption for health benefits, where organic farming is a better solution for the rising population and is even supported by major countries instead of using chemical fertilisers and pesticides. Nanotechnology provides a platform where nanoparticles help in better management for organic farming by using it as nano fertilisers, nanocides, nano biosensors, nano growth promoters, etc. These nanomaterials can be synthesised by three different mechanisms namely; chemical, physical, and biological methods. Since the chemical and physical mode of synthesis does not follow the criteria of organic farming and have their drawbacks. Hence, the biological method, also known as the green synthesis of nanomaterials fulfills the requirement of organic farming and has achieved the attention of researchers. Extracts of plant parts (stems, roots, leaves, flowers and, fruits) and different microbes, including bacteria, fungus, and mycorrhiza can be used as a base material for the synthesis of nanoparticles under green synthesis mode. The vision behind the green synthesis of nanoparticles was to curb the hazardous effects of chemically synthesised nanoparticles. In the present review, green synthesis of major elements of organic farming namely; nano fertilisers, nano-pesticides, and nano growth promoters, their modes of transportation, their advantages, and disadvantages in organic farming are discussed.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47742473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. M. Umlong, Dhiraj Dutta, Rama Dubey, Sanjai K. Dwivedi
A non-electrical filter is designed to cater the need of providing iron free water in forward and rural areas. The features of the unit are equipped with the aeration system and activated sand chemically coated by iron oxide as catalytic and filtering media which can bring down iron concentration from 12 mg/L to desirable limit. It increases the pH of water from acidic to pH above 7. This is in contrast to some indigenous water filter which existed in the North Eastern India that tend to remove iron below 4 mg/L. The modified filter is effective in reducing excess total dissolved solids (TDS) from drinking water. All other physical parameters found to be within the prescribed limit. It can give iron free water with output capacity of 25L/hr. The added advantage of the unit is the provision for back washing of the filter media and collection point of the precipitated iron at the lower bottom of the tank for safe discharge.
{"title":"A Modified Non Electrical Filter for Decontamination of Iron Rich Water for Rural Application","authors":"I. M. Umlong, Dhiraj Dutta, Rama Dubey, Sanjai K. Dwivedi","doi":"10.14429/dlsj.7.17342","DOIUrl":"https://doi.org/10.14429/dlsj.7.17342","url":null,"abstract":"A non-electrical filter is designed to cater the need of providing iron free water in forward and rural areas. The features of the unit are equipped with the aeration system and activated sand chemically coated by iron oxide as catalytic and filtering media which can bring down iron concentration from 12 mg/L to desirable limit. It increases the pH of water from acidic to pH above 7. This is in contrast to some indigenous water filter which existed in the North Eastern India that tend to remove iron below 4 mg/L. The modified filter is effective in reducing excess total dissolved solids (TDS) from drinking water. All other physical parameters found to be within the prescribed limit. It can give iron free water with output capacity of 25L/hr. The added advantage of the unit is the provision for back washing of the filter media and collection point of the precipitated iron at the lower bottom of the tank for safe discharge.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47833904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Allium sativum (garlic) is used as food additives and medicines. Its health benefits are well known, which are mainly contributed by the active organosulfur compounds present in it. Though garlic widely used, but limited is known about preclinical acute toxicity of its organosulfur compounds in mice. The present study aimed at toxicity evaluation of diallyl sulfide (DAS) in C57BL/6 mice following oral administration at range of concentrations (40 mg/kg, 400 mg/kg, 800 mg/kg and 1600 mg/kg). Survival, hematological, organ coefficients, and histopathology studies were performed to establish the DAS toxicity in mice. Stability studies performed in vitro by HPLC showed rapid and time dependent changes in DAS area. A single oral dose upto 1600 mg/kg was well tolerated in mice without any significant changes in standard toxicological parameters. No death was recorded at the tested concentrations. Also no significant changes in the organ coefficient were observed when compared to vehicle treated and sham control. Mild alterations in liver pathology and hematological changes were observed post 1600 mg/kg DAS administration orally. This study demonstrates that single oral administration (upto 1600 mg/kg) of DAS is within the safe limits with no observable adverse effects in mice. Based on the safety profile of DAS, we conclude that DAS can be further explored for use in humans as a potential radiomitigator.
{"title":"Investigation on Oral Toxicity of Diallyl Sulfide A Principle Organosulfur Compound Derived from Allium Sativum Garlic in Mice","authors":"A. Dahiya, A. Prakash, P. Agrawala, A. Dutta","doi":"10.14429/dlsj.7.16972","DOIUrl":"https://doi.org/10.14429/dlsj.7.16972","url":null,"abstract":"Allium sativum (garlic) is used as food additives and medicines. Its health benefits are well known, which are mainly contributed by the active organosulfur compounds present in it. Though garlic widely used, but limited is known about preclinical acute toxicity of its organosulfur compounds in mice. The present study aimed at toxicity evaluation of diallyl sulfide (DAS) in C57BL/6 mice following oral administration at range of concentrations (40 mg/kg, 400 mg/kg, 800 mg/kg and 1600 mg/kg). Survival, hematological, organ coefficients, and histopathology studies were performed to establish the DAS toxicity in mice. Stability studies performed in vitro by HPLC showed rapid and time dependent changes in DAS area. A single oral dose upto 1600 mg/kg was well tolerated in mice without any significant changes in standard toxicological parameters. No death was recorded at the tested concentrations. Also no significant changes in the organ coefficient were observed when compared to vehicle treated and sham control. Mild alterations in liver pathology and hematological changes were observed post 1600 mg/kg DAS administration orally. This study demonstrates that single oral administration (upto 1600 mg/kg) of DAS is within the safe limits with no observable adverse effects in mice. Based on the safety profile of DAS, we conclude that DAS can be further explored for use in humans as a potential radiomitigator.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41608088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Pandey, Anchala Guglani, G. Balakrishna, Vinod Kumar
The variation in antioxidant activity and antioxidant constituents of different plant growth stages i.e. in every 15 day’s interval till maturity of plant of Ocimum basilicum Linn. grown in an open field and inside polyhouse conditions was studied. The correlation between antioxidant constituents and antioxidant activities was also studied. The result revealed that the 90 days old plant grown in an open field condition exhibited maximum antioxidant activity with the lowest IC50/EC50 value by ABTS (0.064 mg/ml), DPPH (0.090 mg/ml), and FRAP (0.099 mg/ml) followed by 75 days and 60 days old plants, similar pattern was also observed in their antioxidant constituents. Similarly, the 90 days old plant grown inside polyhouse condition showed maximum antioxidant activity with antioxidant constituents followed by other plant growth stages in descending order. The chlorophyll content was found maximum in 15 days old plant(0.926 mg/100g) grown in an open field condition, whereas the maximum chlorophyll content (1.470 mg/100g) exhibited by 90 days old plant grown inside polyhouse condition. The correlation analysis revealed that the antioxidant constituents exhibited a significant negative correlation with the IC50/EC50 value and the IC50 value of ABTS assay had a significantly positive correlation with the IC50 and EC50 value of DPPH and FRAP assay, respectively. Hence, the study revealed that the leaves extract of plant grown in open field conditions possesses a significantly higher antioxidant activity and antioxidant constituents than the plant grown inside polyhouse condition.
{"title":"Variation in Antioxidant Activity and Antioxidant Constituents of Ocimum basilicum Linn with the Maturity of Plant Grown in Open Field and Inside Polyhouse Conditions","authors":"H. Pandey, Anchala Guglani, G. Balakrishna, Vinod Kumar","doi":"10.14429/dlsj.7.17247","DOIUrl":"https://doi.org/10.14429/dlsj.7.17247","url":null,"abstract":"The variation in antioxidant activity and antioxidant constituents of different plant growth stages i.e. in every 15 day’s interval till maturity of plant of Ocimum basilicum Linn. grown in an open field and inside polyhouse conditions was studied. The correlation between antioxidant constituents and antioxidant activities was also studied. The result revealed that the 90 days old plant grown in an open field condition exhibited maximum antioxidant activity with the lowest IC50/EC50 value by ABTS (0.064 mg/ml), DPPH (0.090 mg/ml), and FRAP (0.099 mg/ml) followed by 75 days and 60 days old plants, similar pattern was also observed in their antioxidant constituents. Similarly, the 90 days old plant grown inside polyhouse condition showed maximum antioxidant activity with antioxidant constituents followed by other plant growth stages in descending order. The chlorophyll content was found maximum in 15 days old plant(0.926 mg/100g) grown in an open field condition, whereas the maximum chlorophyll content (1.470 mg/100g) exhibited by 90 days old plant grown inside polyhouse condition. The correlation analysis revealed that the antioxidant constituents exhibited a significant negative correlation with the IC50/EC50 value and the IC50 value of ABTS assay had a significantly positive correlation with the IC50 and EC50 value of DPPH and FRAP assay, respectively. Hence, the study revealed that the leaves extract of plant grown in open field conditions possesses a significantly higher antioxidant activity and antioxidant constituents than the plant grown inside polyhouse condition.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48672265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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