G. Bó, E. Huguenine, A. Vera, J. de la Matta, R. D. de Carneiro, A. Menchaca
For years, researchers have been seeking to manipulate sex before conception. Although the use of sexed semen has been of great interest in the past 20 years to inseminate dairy heifers, the massive use of sexed semen was somewhat limited because fertility was compromised and could not be solved by increasing the number of sperm per inseminating dose. However, new simplified and less traumatic procedures for sperm have recently been developed that have significantly improved pregnancy rates obtained with sexed semen. However, for the massive use of this technology it is necessary to develop fixed-time Artificial Insemination (FTAI) programs for sexed semen. Therefore, a series of experiments were carried out on heifers in cows with calf on foot. In all cases the animals were synchronized with the help of paint at the base of the tail to detect the presentation of jealousy and optimize the IATF schedules. Although pregnancy rates were generally higher for conventional semen than for sexed semen, pregnancy rates with sexed semen were 45 to 50%. Therefore, the results of the experiments carried out show that IATF protocols can be adapted for the use of sexed semen and thus enable the massive use of this technology.
{"title":"Programas de fixed-time artificial insemination de alta fertilidad con semen\u0000 sexado","authors":"G. Bó, E. Huguenine, A. Vera, J. de la Matta, R. D. de Carneiro, A. Menchaca","doi":"10.18548/aspe/0010.11","DOIUrl":"https://doi.org/10.18548/aspe/0010.11","url":null,"abstract":"For years, researchers have been seeking to manipulate sex before conception.\u0000 Although the use of sexed semen has been of great interest in the past 20 years to\u0000 inseminate dairy heifers, the massive use of sexed semen was somewhat limited because\u0000 fertility was compromised and could not be solved by increasing the number of sperm per\u0000 inseminating dose. However, new simplified and less traumatic procedures for sperm have\u0000 recently been developed that have significantly improved pregnancy rates obtained with\u0000 sexed semen. However, for the massive use of this technology it is necessary to develop\u0000 fixed-time Artificial Insemination (FTAI) programs for sexed semen. Therefore, a series\u0000 of experiments were carried out on heifers in cows with calf on foot. In all cases the\u0000 animals were synchronized with the help of paint at the base of the tail to detect the\u0000 presentation of jealousy and optimize the IATF schedules. Although pregnancy rates were\u0000 generally higher for conventional semen than for sexed semen, pregnancy rates with sexed\u0000 semen were 45 to 50%. Therefore, the results of the experiments carried out show that\u0000 IATF protocols can be adapted for the use of sexed semen and thus enable the massive use\u0000 of this technology.","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44253333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Equine chorionic gonatrophin (eCG) is a high molecular weight glycoprotein produced by endometrial cups in the mare. ECG has primarily LH activity in the mare, but has FSH or LH activity in the cow, depending on the recipient populations in the ovary. Although eCG has been used to induce superovulation in a variety of species, several studies have demonstrated the beneficial effects of eCG on fixed-time AI (IATF) programs in beef and dairy cattle, and embryo recipients. Under these circumstances, eCG has stimulated the development of the ovulatory dominant follicle and produced ovulation of a larger follicle, resulting in a larger and more functional LC and longer embryo survival. In suckling beef cows, 400 IU of eCG at the time of removal of a progesterone device resulted in increased conception rates after IATF,and in dairy cattle managed in pastoral systems, the eCG administration resulted in a significant increase in pregnancy rates, especially in cows in anestrus. In embryo recipients, treatment with eCG has resulted in increased pregnancy rates after embryo transfer. This presentation will review some of this data and explore strategies to use the potential benefits of eCG to improve bovine reproduction.
{"title":"Physiology related to the use of eCG in beef and dairy cattle","authors":"R. Mapletoft, P. Baruselli, G. Bó","doi":"10.18548/aspe/0010.13","DOIUrl":"https://doi.org/10.18548/aspe/0010.13","url":null,"abstract":"Equine chorionic gonatrophin (eCG) is a high molecular weight glycoprotein\u0000 produced by endometrial cups in the mare. ECG has primarily LH activity in the mare, but\u0000 has FSH or LH activity in the cow, depending on the recipient populations in the ovary.\u0000 Although eCG has been used to induce superovulation in a variety of species, several\u0000 studies have demonstrated the beneficial effects of eCG on fixed-time AI (IATF) programs\u0000 in beef and dairy cattle, and embryo recipients. Under these circumstances, eCG has\u0000 stimulated the development of the ovulatory dominant follicle and produced ovulation of\u0000 a larger follicle, resulting in a larger and more functional LC and longer embryo\u0000 survival. In suckling beef cows, 400 IU of eCG at the time of removal of a progesterone\u0000 device resulted in increased conception rates after IATF,and in dairy cattle managed in\u0000 pastoral systems, the eCG administration resulted in a significant increase in pregnancy\u0000 rates, especially in cows in anestrus. In embryo recipients, treatment with eCG has\u0000 resulted in increased pregnancy rates after embryo transfer. This presentation will\u0000 review some of this data and explore strategies to use the potential benefits of eCG to\u0000 improve bovine reproduction.","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44548013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Clara Canto Souza, Gabrieli Dutra Gonçalves, Andrey Osvaldo Souza Ferro, Deborah Nakayama Yokomizo, Denis Vinicius Bonato, M. Marcondes Seneda
The application of Fixed Time Artificial Insemination (FTAI) protocols has strongly boosted artificial insemination in Brazilian bovine herds. The control of the estrous cycle with protocols based on progesterone and estrogen involves synchronizing the emergence of a follicular wave, controlling the progesterone phase, and inducing ovulation in a synchronized manner. The increase in pregnancy rates starts with the preparation of the breeding season and the choice of protocols and females. Thereafter, based on the characteristics of the property and the lots of animals, small adjustments can be made to the hormonal protocols as needed. Finally, resynchronization can increase usability by allowing multiple inseminations within short periods. The search for new strategies is of paramount importance for the development of the FTAI protocol and the increase in final pregnancy rates. Thus, this review sought to highlight the main critical points and adjustments of the FTAI protocols and their applicability in the reproduction of beef cattle.
{"title":"STRATEGIES TO INCREASE FERTILITY DURING FIXED-TIME ARTIFICIAL INSEMINATION IN BEEF CATTLE","authors":"Ana Clara Canto Souza, Gabrieli Dutra Gonçalves, Andrey Osvaldo Souza Ferro, Deborah Nakayama Yokomizo, Denis Vinicius Bonato, M. Marcondes Seneda","doi":"10.18548/aspe/0009.11","DOIUrl":"https://doi.org/10.18548/aspe/0009.11","url":null,"abstract":"The application of Fixed Time Artificial Insemination (FTAI) protocols has strongly boosted artificial insemination in Brazilian bovine herds. The control of the estrous cycle with protocols based on progesterone and estrogen involves synchronizing the emergence of a follicular wave, controlling the progesterone phase, and inducing ovulation in a synchronized manner. The increase in pregnancy rates starts with the preparation of the breeding season and the choice of protocols and females. Thereafter, based on the characteristics of the property and the lots of animals, small adjustments can be made to the hormonal protocols as needed. Finally, resynchronization can increase usability by allowing multiple inseminations within short periods. The search for new strategies is of paramount importance for the development of the FTAI protocol and the increase in final pregnancy rates. Thus, this review sought to highlight the main critical points and adjustments of the FTAI protocols and their applicability in the reproduction of beef cattle.","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44766887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Akshay Sharma, Madhumeet Singh, P. Kumar, Surender Kumar, R. Thakur
Forty-one dairy cows (n=41) were enrolled to envisage the changes in uterine haemodynamics during sub-clinical endometritis (SCE) and its treatment with modified estrus synchronization protocols at 8 weeks post-partum. Trans-rectal Doppler sonography of both the middle uterine arteries (MUAs) was carried out for assessment of uterine perfusion whereas serum inflammatory markers i.e. IL-6 and C-RP were measured at 8 weeks post-partum and estrus (induced or spontaneous). Modified estrus synchronization protocols (MG6G and MG6GP) were used to adjudge their efficacy in post-partum dairy cows diagnosed with SCE and reproductive parameters were recorded. As a part of result, Doppler indices of both the MUAs at estrus i.e. TAMEAN, TAMAX, Blood flow volume-TAMEAN and TAMAX and diameter of MUA, were significantly lower (P<0.05) after application of MG6G and MG6GP protocols in SCEP as compared to SCEP control cows. Similarly, the IL-6, C-RP concentrations and PMNCs proportion (%) were significantly higher (P<0.05) in SCEP control as compared to cows treated with MG6G and MG6GP protocols. Moreover, on the day of estrus, the uterine haemodynamics, concentration of serum inflammatory markers and PMNCs proportion (%) in treated cows was at par with SCE negative control (SCEN) cows. In terms of reproductive performance, days open were recorded to be significantly lower (P<0.01) in treated and SCEN group as compared to SCEP control cows. In conclusion, sub-clinical endometritis led higher uterine perfusion, release of proinflammatory cytokines and PMNCs proportion which happened to plummet the post-partum reproductive performance was successfully managed with modified estrus synchronization protocols.
{"title":"POST-PARTUM SUB-CLINICAL ENDOMETRITIS- ALTERATION IN UTERINE HAEMODYNAMICS, INFLAMMATORY MARKERS ON TREATMENT WITH MODIFIED ESTRUS SYNCHRONIZATION PROTOCOLS IN DAIRY COWS","authors":"Akshay Sharma, Madhumeet Singh, P. Kumar, Surender Kumar, R. Thakur","doi":"10.18548/aspe/0009.17","DOIUrl":"https://doi.org/10.18548/aspe/0009.17","url":null,"abstract":"Forty-one dairy cows (n=41) were enrolled to envisage the changes in uterine haemodynamics during sub-clinical endometritis (SCE) and its treatment with modified estrus synchronization protocols at 8 weeks post-partum. Trans-rectal Doppler sonography of both the middle uterine arteries (MUAs) was carried out for assessment of uterine perfusion whereas serum inflammatory markers i.e. IL-6 and C-RP were measured at 8 weeks post-partum and estrus (induced or spontaneous). Modified estrus synchronization protocols (MG6G and MG6GP) were used to adjudge their efficacy in post-partum dairy cows diagnosed with SCE and reproductive parameters were recorded. As a part of result, Doppler indices of both the MUAs at estrus i.e. TAMEAN, TAMAX, Blood flow volume-TAMEAN and TAMAX and diameter of MUA, were significantly lower (P<0.05) after application of MG6G and MG6GP protocols in SCEP as compared to SCEP control cows. Similarly, the IL-6, C-RP concentrations and PMNCs proportion (%) were significantly higher (P<0.05) in SCEP control as compared to cows treated with MG6G and MG6GP protocols. Moreover, on the day of estrus, the uterine haemodynamics, concentration of serum inflammatory markers and PMNCs proportion (%) in treated cows was at par with SCE negative control (SCEN) cows. In terms of reproductive performance, days open were recorded to be significantly lower (P<0.01) in treated and SCEN group as compared to SCEP control cows. In conclusion, sub-clinical endometritis led higher uterine perfusion, release of proinflammatory cytokines and PMNCs proportion which happened to plummet the post-partum reproductive performance was successfully managed with modified estrus synchronization protocols.","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44198020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study aimed to compare results for hatchability and growing performance of chicks from 2 biotypes, dual purpose (DP) and brawl (BR), in hipobaric conditions (2718 m above sea level). In experiment 1, a total of 352 hatching eggs were randomly arranged, 176 egg of DP y 176 BR. In experiment 2, a total of 114 one-day-old unsexed chickens were reared for 28 d. The experimental design for hatchability and chick performance variables was completely randomized with 2 treatments (DP and BR). There were no differences between the studied treatments for hatchability results (p>0.05). Hens’ biotype affected chicken performance in the rearing phase (p<0.01), where the DP chickens had better body weight and feed conversion than BR, but BR chickens had lower mortality than DP chickens.
本研究旨在比较两种生物型，即双用途（DP）和肌肉型（BR）在海平面2718m的条件下的孵化率和生长性能。在实验1中，共随机安排了352个孵化蛋，其中176个蛋为DP y 176 BR。在实验2中，共饲养了114只一日龄未交配的鸡28天。孵化率和鸡性能变量的实验设计完全随机分为两个处理（DP和BR）。所研究的处理之间的孵化率结果没有差异（p>0.05）。母鸡的生物类型在饲养阶段影响鸡的性能（p<0.01），其中DP鸡比BR鸡有更好的体重和饲料转化率，但BR鸡比DP鸡有更低的死亡率。
{"title":"Hatching, embryo death and chicks growth in dual purpose and brawl hens under natural hypobaric conditions","authors":"M. Paredes, Talía Quispe","doi":"10.18548/aspe/0009.19","DOIUrl":"https://doi.org/10.18548/aspe/0009.19","url":null,"abstract":"The present study aimed to compare results for hatchability and growing performance of chicks from 2 biotypes, dual purpose (DP) and brawl (BR), in hipobaric conditions (2718 m above sea level). In experiment 1, a total of 352 hatching eggs were randomly arranged, 176 egg of DP y 176 BR. In experiment 2, a total of 114 one-day-old unsexed chickens were reared for 28 d. The experimental design for hatchability and chick performance variables was completely randomized with 2 treatments (DP and BR). There were no differences between the studied treatments for hatchability results (p>0.05). Hens’ biotype affected chicken performance in the rearing phase (p<0.01), where the DP chickens had better body weight and feed conversion than BR, but BR chickens had lower mortality than DP chickens.","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45412489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Pierr Manrique Quispe, Carlos Washington Bustamante Quispe, Francisco Rodríguez Huanca, Domingo Ruelas Calloapaza, U. P. Pérez Guerra, Marcelino Araníbar Araníbar, M. P. Pérez Durand
Trout production in Peru has export potential. However, there are no fry available to maintain production throughout the year and there is also a lack of egg production at certain times of the year. Therefore, the alternative is to cryopreserve semen for the non-reproductive season. The objective of the study was to determine the influence of freezing temperature on the quality of rainbow trout semen. The semen of 12 reproductive males was collected in the facilities of the Chucuito Research and Production Center of the National University of the Altiplano of Puno, which underwent a macroscopic, microscopic pre and post thaw seminal evaluation and the fertility rate was measured. Due to the effect of three freezing temperatures (-80 °C, -100 °C, -120 °C), cryopreservation had a decline curve of -20 °C / min. The fresh seminal parameters were similar to those reported by other researchers. While freezing had unfavorable effects on semen quality, the best results for activation time (51.33 sec) and vitality (35.33%) were obtained with -100 °C, but the higher motility was obtained with -120 °C (36.33%). Regarding fertility, the higher rate was obtained with -100 °C (70.97%), followed by -80 °C and -120 °C in which 68.86% and 64.34% were obtained, respectively. In conclusion, the results suggest that the tolerable freezing temperature of rainbow trout semen is around -100 °C, which is shown as a favorable alternative for the reproductive management of rainbow trout under natural hypobaric conditions of the Peruvian highlands
{"title":"Influence of freezing temperature on semen quality of rainbow trout (Oncorhynchus mykiss)","authors":"Yan Pierr Manrique Quispe, Carlos Washington Bustamante Quispe, Francisco Rodríguez Huanca, Domingo Ruelas Calloapaza, U. P. Pérez Guerra, Marcelino Araníbar Araníbar, M. P. Pérez Durand","doi":"10.18548/aspe/0009.21","DOIUrl":"https://doi.org/10.18548/aspe/0009.21","url":null,"abstract":"Trout production in Peru has export potential. However, there are no fry available to maintain production throughout the year and there is also a lack of egg production at certain times of the year. Therefore, the alternative is to cryopreserve semen for the non-reproductive season. The objective of the study was to determine the influence of freezing temperature on the quality of rainbow trout semen. The semen of 12 reproductive males was collected in the facilities of the Chucuito Research and Production Center of the National University of the Altiplano of Puno, which underwent a macroscopic, microscopic pre and post thaw seminal evaluation and the fertility rate was measured. Due to the effect of three freezing temperatures (-80 °C, -100 °C, -120 °C), cryopreservation had a decline curve of -20 °C / min. The fresh seminal parameters were similar to those reported by other researchers. While freezing had unfavorable effects on semen quality, the best results for activation time (51.33 sec) and vitality (35.33%) were obtained with -100 °C, but the higher motility was obtained with -120 °C (36.33%). Regarding fertility, the higher rate was obtained with -100 °C (70.97%), followed by -80 °C and -120 °C in which 68.86% and 64.34% were obtained, respectively. In conclusion, the results suggest that the tolerable freezing temperature of rainbow trout semen is around -100 °C, which is shown as a favorable alternative for the reproductive management of rainbow trout under natural hypobaric conditions of the Peruvian highlands","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47258551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ide Unchupaico Payano, Edith Ancco Gomez, Eduardo Fernández Curi, Williams Olivera Acuña, Carlos Quispe Eulogio
This study aimed to determine protein and energy metabolites in postpartum Brown Swiss cows and their effect on the presence of subclinical ketosis and reproductive parameters. One hundred cows from four farms in the Mantaro Valley (Junín, Perú) were grouped according to parity (1, 2, 3 and 4). The variables of the metabolic profile (total proteins, blood urea nitrogen [BUN], glucose, βhydroxybutyrate [B-HB]), milk production and body condition were estimated between 7 to 60 days postpartum in 7-day intervals. Likewise, the calving-first heat interval and the pregnancy rate at first service were recorded. The determination of subclinical ketosis was based on a semi-quantitative analysis by levels of B-HB in milk (µmol/l) using a commercial kit. BUN levels ranged between 11.74 and 15.92 mg/dl, being higher in fourth parity cows (p<0.05). The averages of total protein (6.54-7.90 g/dl) were homogeneous between calvings. The glucose values presented an inverse response, being lower in cows of third and fourth parity compared to cows of first and second parity (p<0.05). Similarly, the highest levels of milk production were observed in third parity cows (14.41 ± 5.42 l/d) and fourth parity (15.43 ± 4.36 l/d) with respect to first and second calvers (p<0.05). Body condition was lower in cows with subclinical ketosis. The calving - first heat interval and the pregnancy rate at first service were lower in cows with subclinical ketosis (p<0.05).
{"title":"Effects of energy and protein status on reproductive parameters and presence of subclinical ketosis in Brown Swiss cows postpartum","authors":"Ide Unchupaico Payano, Edith Ancco Gomez, Eduardo Fernández Curi, Williams Olivera Acuña, Carlos Quispe Eulogio","doi":"10.18548/aspe/0009.18","DOIUrl":"https://doi.org/10.18548/aspe/0009.18","url":null,"abstract":"This study aimed to determine protein and energy metabolites in postpartum Brown Swiss cows and their effect on the presence of subclinical ketosis and reproductive parameters. One hundred cows from four farms in the Mantaro Valley (Junín, Perú) were grouped according to parity (1, 2, 3 and 4). The variables of the metabolic profile (total proteins, blood urea nitrogen [BUN], glucose, βhydroxybutyrate [B-HB]), milk production and body condition were estimated between 7 to 60 days postpartum in 7-day intervals. Likewise, the calving-first heat interval and the pregnancy rate at first service were recorded. The determination of subclinical ketosis was based on a semi-quantitative analysis by levels of B-HB in milk (µmol/l) using a commercial kit. BUN levels ranged between 11.74 and 15.92 mg/dl, being higher in fourth parity cows (p<0.05). The averages of total protein (6.54-7.90 g/dl) were homogeneous between calvings. The glucose values presented an inverse response, being lower in cows of third and fourth parity compared to cows of first and second parity (p<0.05). Similarly, the highest levels of milk production were observed in third parity cows (14.41 ± 5.42 l/d) and fourth parity (15.43 ± 4.36 l/d) with respect to first and second calvers (p<0.05). Body condition was lower in cows with subclinical ketosis. The calving - first heat interval and the pregnancy rate at first service were lower in cows with subclinical ketosis (p<0.05).","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44873963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hasanain, K. Mahmoud, Y. Ahmed, A. Sakr, A. EL-Menoufy, S. Ismail
This work aimed to investigate the genetic polymorphism of Aquaporin 7 (AQP7) gene and its association with the in-vivo fertility of Egyptian buffalo bulls. A total of 188 fresh semen ejaculates were collected from 47 buffalo bulls and subjected to semen evaluation. The bulls were grouped according to in-vivo fertility relying on sire conception rate (SCR) into high (SCR>50%, n=41) and low (SCR<50%, n=6) fertile bulls. DNA was extracted from semen and a 200 bp fragment of the AQP7 gene was amplified by PCR. The genetic polymorphism of AQP7 gene was detected by restriction fragment length polymorphism, the single strand conformation polymorphism and the nucleotide sequencing. The results revealed that SCR was significantly (p<0.001) increased in high (71.4±1.3) than low (44.7±2.8) fertile bulls while the ejaculate volume, individual motility%, lives sperm%, sperm concentration and sperm abnormalities showed a non-significant difference in both groups. The AQP7 gene showed no genetic variation in all bulls. The alignment of the resultant sequence with buffalo AQP7 gene sequence (ID: XM_006066699.2) showed 100% identity (122/122), however, deletion of A49 was discovered when the sequence was aligned with ID: XM_006066700.2 of buffalo in GenBank. In conclusion, the sire conception rate is not associated with any genetic variation in Aquaporin 7 gene. The Aquaporin 7 gene could be a highly conserved sequence in Egyptian buffalo bulls. Future research on large herd populations in different regions of AQP7 gene is required for evaluating the AQP7 gene polymorphism and its association with the fertility of Egyptian buffalo bulls.
{"title":"IDENTIFICATION OF AQUAPORIN 7 GENE POLYMORPHISM AND IN-VIVO FERTILITY OF EGYPTIAN BUFFALO BULLS","authors":"M. Hasanain, K. Mahmoud, Y. Ahmed, A. Sakr, A. EL-Menoufy, S. Ismail","doi":"10.18548/aspe/0009.14","DOIUrl":"https://doi.org/10.18548/aspe/0009.14","url":null,"abstract":"This work aimed to investigate the genetic polymorphism of Aquaporin 7 (AQP7) gene and its association with the in-vivo fertility of Egyptian buffalo bulls. A total of 188 fresh semen ejaculates were collected from 47 buffalo bulls and subjected to semen evaluation. The bulls were grouped according to in-vivo fertility relying on sire conception rate (SCR) into high (SCR>50%, n=41) and low (SCR<50%, n=6) fertile bulls. DNA was extracted from semen and a 200 bp fragment of the AQP7 gene was amplified by PCR. The genetic polymorphism of AQP7 gene was detected by restriction fragment length polymorphism, the single strand conformation polymorphism and the nucleotide sequencing. The results revealed that SCR was significantly (p<0.001) increased in high (71.4±1.3) than low (44.7±2.8) fertile bulls while the ejaculate volume, individual motility%, lives sperm%, sperm concentration and sperm abnormalities showed a non-significant difference in both groups. The AQP7 gene showed no genetic variation in all bulls. The alignment of the resultant sequence with buffalo AQP7 gene sequence (ID: XM_006066699.2) showed 100% identity (122/122), however, deletion of A49 was discovered when the sequence was aligned with ID: XM_006066700.2 of buffalo in GenBank. In conclusion, the sire conception rate is not associated with any genetic variation in Aquaporin 7 gene. The Aquaporin 7 gene could be a highly conserved sequence in Egyptian buffalo bulls. Future research on large herd populations in different regions of AQP7 gene is required for evaluating the AQP7 gene polymorphism and its association with the fertility of Egyptian buffalo bulls.","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44783787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was developed at the Pampa del Arco Experimental Center of the National University of San Cristóbal de Huamanga, the objective was to evaluate doses of 800 IU (T1) and doses of 1000 IU (T2) of eCG to obtain embryos. Eight adult female llamas were used, distributed four in each treatment (T1 and T2), with a body condition of 2.5, these were evaluated by ultrasound with a 7.5 MHz rectal linear transducer. Hormonal treatments began when the animals had a follicle> 7 mm. The application of eCG was 72 hours after the induction of ovulation of the dominant follicle with GnRH, on day 7 PGF2α was applied, natural mounting was performed plus GnRH (1ml), on day 15 the collection and evaluation of embryos was performed. The results obtained were the pre-ovarian stimulation follicular diameter of 8.3 mm and 9.1 mm for T1 and T2 respectively, the number of pre-ovulatory follicles was 6.33 and 5.50 for T1 and T2 respectively, the diameter of follicles pre-ovulatory was 11.2mm and 10.6 mm for T1 and T2 respectively, average of embryos recovered per donor was 4.66 ± 0.81 and 3.50 ± 0.54 embryos for T1 and T2 respectively, with a significant difference (p <0.05) and the quality of embryos had 35.7% and 38.1% of embryos of excellent quality, 28.6% and 33.3% of good quality, 10.7% and 9 , 5% of regular quality, 10.7% and 0% of poor quality, for T1 and T2 respectively and 14.3% and 19.0% of non-transferable embryos for T1 and T2 respectively, with no statistical difference (p≥0, 05). It is concluded that the number of embryos recovered with a dose of 800 IU of eCG is higher compared to the dose of 1000 IU of eCG (p <0.05) and that there is no association between the applied dose of eCG and the quality of the llama embryos (p≥0.05)
这项研究是在San国立大学Cristóbal de Huamanga Pampa del Arco实验中心开展的，目的是评估800 IU (T1)剂量和1000 IU (T2)剂量的eCG获得胚胎的效果。选用成年雌性大羊驼8只，体况2.5,T1和T2各4只，采用7.5 MHz直肠线性换向器进行超声检测。激素治疗开始时，动物的卵泡有一个70毫米。在GnRH诱导优势卵泡排卵后72h应用eCG，第7天应用PGF2α，加GnRH (1ml)自然贴装，第15天收集和评估胚胎。结果:T1和T2促卵巢前卵泡直径分别为8.3 mm和9.1 mm, T1和T2促排卵前卵泡数分别为6.33和5.50个，T1和T2促排卵前卵泡直径分别为11.2mm和10.6 mm, T1和T2平均每个供体回收的胚胎分别为4.66±0.81和3.50±0.54个。，差异有统计学意义(p <0.05)， T1和T2的优良率分别为35.7%和38.1%，优良率分别为28.6%和33.3%，正常率分别为10.7%和9.5%，差质率分别为10.7%和0%，T1和T2的不可移植率分别为14.3%和19.0%，差异无统计学意义(p≥0.05)。综上所述，800 IU剂量下恢复的胚胎数量高于1000 IU剂量(p <0.05)， eCG剂量与羊驼胚胎质量无相关性(p≥0.05)。
{"title":"Comparison of two doses of eCG for obtaining embryos in llama","authors":"Cesar Olaguivel, J. Ruíz, Pedro U. Coila","doi":"10.18548/aspe/0009.23","DOIUrl":"https://doi.org/10.18548/aspe/0009.23","url":null,"abstract":"The study was developed at the Pampa del Arco Experimental Center of the National University of San Cristóbal de Huamanga, the objective was to evaluate doses of 800 IU (T1) and doses of 1000 IU (T2) of eCG to obtain embryos. Eight adult female llamas were used, distributed four in each treatment (T1 and T2), with a body condition of 2.5, these were evaluated by ultrasound with a 7.5 MHz rectal linear transducer. Hormonal treatments began when the animals had a follicle> 7 mm. The application of eCG was 72 hours after the induction of ovulation of the dominant follicle with GnRH, on day 7 PGF2α was applied, natural mounting was performed plus GnRH (1ml), on day 15 the collection and evaluation of embryos was performed. The results obtained were the pre-ovarian stimulation follicular diameter of 8.3 mm and 9.1 mm for T1 and T2 respectively, the number of pre-ovulatory follicles was 6.33 and 5.50 for T1 and T2 respectively, the diameter of follicles pre-ovulatory was 11.2mm and 10.6 mm for T1 and T2 respectively, average of embryos recovered per donor was 4.66 ± 0.81 and 3.50 ± 0.54 embryos for T1 and T2 respectively, with a significant difference (p <0.05) and the quality of embryos had 35.7% and 38.1% of embryos of excellent quality, 28.6% and 33.3% of good quality, 10.7% and 9 , 5% of regular quality, 10.7% and 0% of poor quality, for T1 and T2 respectively and 14.3% and 19.0% of non-transferable embryos for T1 and T2 respectively, with no statistical difference (p≥0, 05). It is concluded that the number of embryos recovered with a dose of 800 IU of eCG is higher compared to the dose of 1000 IU of eCG (p <0.05) and that there is no association between the applied dose of eCG and the quality of the llama embryos (p≥0.05)","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43339714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Post-partum uterine infections such as metritis, clinical endometritis and sub-clinical endometritis, are the most common cause for decreased productivity and fertility in dairy cows. Being the least severe form of endometritis, sub-clinical endometritis (SCE) is defined as the superficial inflammation of endometrium with no signs of systemic illness and characterized by an increase in number of polymorphonuclear cells (PMNCs) inside the uterine lumen. The impact of sub-clinical endometritis on fertility of dairy cows is well known probably due to absence of any clinical signs and thus, difficulty in diagnosis and treatment. Different techniques such as endometrial cytology, uterine biopsy, biochemical analysis of uterine fluid, and measurement of acute phase proteins and inflammatory markers have been employed for the diagnosis of SCE. Doppler and B-mode ultrasonography of middle uterine arteries and uterus have also been used to diagnose the inflammation via assessment of uterine perfusion, respectively. Among all methods, endometrial cytology via cytotape is one of the most advanced and frequently employed methods for diagnosis of cytological endometritis based on the fact that proportion of PMNCs increase during uterine inflammation. The review focuses mainly on current status of incidence and diagnosis of post-partum sub-clinical endometritis in dairy cows.
{"title":"POST-PARTUM SUB-CLINICAL ENDOMETRITIS IN DAIRY COWS: INCIDENCE AND DIAGNOSIS","authors":"Akshay Sharma, Madhumeet Singh","doi":"10.18548/aspe/0009.01","DOIUrl":"https://doi.org/10.18548/aspe/0009.01","url":null,"abstract":"Post-partum uterine infections such as metritis, clinical endometritis and sub-clinical endometritis, are the most common cause for decreased productivity and fertility in dairy cows. Being the least severe form of endometritis, sub-clinical endometritis (SCE) is defined as the superficial inflammation of endometrium with no signs of systemic illness and characterized by an increase in number of polymorphonuclear cells (PMNCs) inside the uterine lumen. The impact of sub-clinical endometritis on fertility of dairy cows is well known probably due to absence of any clinical signs and thus, difficulty in diagnosis and treatment. Different techniques such as endometrial cytology, uterine biopsy, biochemical analysis of uterine fluid, and measurement of acute phase proteins and inflammatory markers have been employed for the diagnosis of SCE. Doppler and B-mode ultrasonography of middle uterine arteries and uterus have also been used to diagnose the inflammation via assessment of uterine perfusion, respectively. Among all methods, endometrial cytology via cytotape is one of the most advanced and frequently employed methods for diagnosis of cytological endometritis based on the fact that proportion of PMNCs increase during uterine inflammation. The review focuses mainly on current status of incidence and diagnosis of post-partum sub-clinical endometritis in dairy cows.","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48592996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：