A rapid assessment of spider diversity in Kabigan Falls, Pagudpud, Ilocos Norte. Spiders are the most permeating predators in both agricultural and natural ecosystems. In ecological studies, they serve as indicators of environmental quality and as biological control agents in agricultural ecosystems. A total of 38 individuals collected from Kabigan Falls were comprised of 13 species. These species are from the family Araneidae, Sparassidae, Pholcidae, Salticidae, and Tetragnathidae. Most of the specimens collected from the site were under families Tetragnathidae and Araneidae. The most species-rich family is Araneidae (5). These include the following: Araneus inustus, Cyclosa insulana, Cyclosa mulmeinensis, Cyclosa spirifera and Larinia sp. Newly-recorded spiders include are Tetragnatha plena Chamberlin, 1924, a new Philippine record; and new locality distributed spiders are Chalcotropis luceroi Barrion & Litsinger, 1995 and Pholcus arayat Huber, 2011. KEYWORDS: spiders; Kabigan Falls; spider diversity; taxonomy; Araneae; Tetragnathidae
{"title":"A RAPID ASSESSMENT OF SPIDER DIVERSITY IN KABIGAN FALLS, PAGUDPUD, ILOCOS NORTE, PHILIPPINES","authors":"J. C. Chua, M. Uba, Thaddeus M. Carvajal","doi":"10.3860/PJSB.V8I0.3327","DOIUrl":"https://doi.org/10.3860/PJSB.V8I0.3327","url":null,"abstract":"A rapid assessment of spider diversity in Kabigan Falls, Pagudpud, Ilocos Norte. Spiders are the most permeating predators in both agricultural and natural ecosystems. In ecological studies, they serve as indicators of environmental quality and as biological control agents in agricultural ecosystems. A total of 38 individuals collected from Kabigan Falls were comprised of 13 species. These species are from the family Araneidae, Sparassidae, Pholcidae, Salticidae, and Tetragnathidae. Most of the specimens collected from the site were under families Tetragnathidae and Araneidae. The most species-rich family is Araneidae (5). These include the following: Araneus inustus, Cyclosa insulana, Cyclosa mulmeinensis, Cyclosa spirifera and Larinia sp. Newly-recorded spiders include are Tetragnatha plena Chamberlin, 1924, a new Philippine record; and new locality distributed spiders are Chalcotropis luceroi Barrion & Litsinger, 1995 and Pholcus arayat Huber, 2011. KEYWORDS: spiders; Kabigan Falls; spider diversity; taxonomy; Araneae; Tetragnathidae","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"8 1","pages":"16-26"},"PeriodicalIF":0.0,"publicationDate":"2015-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recent studies on Canthium revealed that the genus is polyphyletic. Subsequently, Canthium sensu stricto ( s.s. ) was re-delimited to species having paired supra-axillary spines, which led to the reinstatement of various genera in the Vanguerieae such as Keetia, Psydrax and Pyrostria . This raised questions on the generic affiliations of Philippine Canthium species as most of its members lacked spines. In an effort to revise the Philippine Canthium , the endemic spineless Canthium subsessilifolium was collected to ascertain its generic affiliation within the tribe using plastid ( trnL-F region) and nuclear (ITS region) markers. The majority-rule consensus tree of combined data sets showed a wellsupported clade of the whole Vanguerieae (PP=1.00). Interestingly, C. subsessilifolium was nested within the robustly supported Pyrostria clade (PP=1.00). Morphological similarities of C. subsessilifolium with Pyrostria further supported our molecular results. Therefore, a new combination is here provided: Pyrostria subsessilifolia (Merr.) Arriola & Alejandro. This study establishes for the first time the existence of Pyrostria in the Philippines. Keywords : Canthium; ITS; Pyrostria; Rubiaceae; trnL-F; Vanguerieae
最近的研究表明，该属是多系的。随后，Canthium sensu stricto (s.s。)被重新划分为具有配对腋窝上棘的种，这导致了先锋科中各种属的恢复，如Keetia, Psydrax和Pyrostria。这引起了对菲律宾Canthium物种的归属问题，因为它的大多数成员缺乏刺。为了修订菲律宾Canthium，我们收集了菲律宾特有的无棘Canthium subsessilifolium，利用叶绿体(trnL-F区)和核(its区)标记确定其在部落中的属属关系。组合数据集的多数规则共识树显示整个前卫的分支得到了很好的支持(PP=1.00)。有趣的是，C. subsessilifolium被嵌套在坚固支撑的火虫枝中(PP=1.00)。在形态学上与火孢菌的相似性进一步支持了我们的分子结果。因此，这里提供了一个新的组合:亚sessilifolia (Merr。Arriola & Alejandro。本研究首次证实菲律宾有火虫。关键词:蛇皮;它的;Pyrostria;茜草科;trnL-F;Vanguerieae
{"title":"A New Record Of Pyrostria (Vanguerieae-Rubiaceae) From The Philippines Inferred from Molecular and Morphological Data","authors":"G. Alejandro, E. Arenas, M. C. Cremen, A. Arriola","doi":"10.3860/PJSB.V7I0.3230","DOIUrl":"https://doi.org/10.3860/PJSB.V7I0.3230","url":null,"abstract":"Recent studies on Canthium revealed that the genus is polyphyletic. Subsequently, Canthium sensu stricto ( s.s. ) was re-delimited to species having paired supra-axillary spines, which led to the reinstatement of various genera in the Vanguerieae such as Keetia, Psydrax and Pyrostria . This raised questions on the generic affiliations of Philippine Canthium species as most of its members lacked spines. In an effort to revise the Philippine Canthium , the endemic spineless Canthium subsessilifolium was collected to ascertain its generic affiliation within the tribe using plastid ( trnL-F region) and nuclear (ITS region) markers. The majority-rule consensus tree of combined data sets showed a wellsupported clade of the whole Vanguerieae (PP=1.00). Interestingly, C. subsessilifolium was nested within the robustly supported Pyrostria clade (PP=1.00). Morphological similarities of C. subsessilifolium with Pyrostria further supported our molecular results. Therefore, a new combination is here provided: Pyrostria subsessilifolia (Merr.) Arriola & Alejandro. This study establishes for the first time the existence of Pyrostria in the Philippines. Keywords : Canthium; ITS; Pyrostria; Rubiaceae; trnL-F; Vanguerieae","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"7 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2014-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two-hundred and twenty-five mangrove bacterial strains previously isolated from several mangrove areas in the Philippines were qualitatively screened for cellulase production and 154 were found to be capable of cellulase production. Among them, ten strains showed very strong positive cellulase activities, which were then quantified. Five with the statistically highest activities, specifically, BBCS-11, BBCS-14, BBoB2L2-2, BOrMGS-2, and BOrMGS-3, including Cellulomonas sp. BIOTECH 1240 (control), were further subjected to partial optimization studies. The effects of substrate, pH, incubation temperature and incubation time on cellulase productoin were determined. The cellulase activities (U mL -1 ) of the strains under optimized conditions were: 54.80 (Control); 56.60 (BBCS-11); 66.50 (BBCS-14); 50.33 (BBoB2L2-2); 51.04 (BOrMGS-2); and 48.70 (BOrMGS-3). The promising cellulase producers were previously identified: BBCS-11 as Bacillus cereus; BBCS-14 as Bacillus licheniformis; BOrMGS-2 and BOrMGS-3 as Bacillus pumilus; and BBoB2L2-2 as Bacillus sp.
{"title":"SCREENING AND OPTIMIZATION OF CELLULASE PRODUCTION OF BACILLUS STRAINS ISOLATED FROM PHILIPPINE MANGROVES","authors":"Nik Shawn C. Tabao, R. Monsalud","doi":"10.3860/PJSB.V4I0.1566","DOIUrl":"https://doi.org/10.3860/PJSB.V4I0.1566","url":null,"abstract":"Two-hundred and twenty-five mangrove bacterial strains previously isolated from several mangrove areas in the Philippines were qualitatively screened for cellulase production and 154 were found to be capable of cellulase production. Among them, ten strains showed very strong positive cellulase activities, which were then quantified. Five with the statistically highest activities, specifically, BBCS-11, BBCS-14, BBoB2L2-2, BOrMGS-2, and BOrMGS-3, including Cellulomonas sp. BIOTECH 1240 (control), were further subjected to partial optimization studies. The effects of substrate, pH, incubation temperature and incubation time on cellulase productoin were determined. The cellulase activities (U mL -1 ) of the strains under optimized conditions were: 54.80 (Control); 56.60 (BBCS-11); 66.50 (BBCS-14); 50.33 (BBoB2L2-2); 51.04 (BOrMGS-2); and 48.70 (BOrMGS-3). The promising cellulase producers were previously identified: BBCS-11 as Bacillus cereus; BBCS-14 as Bacillus licheniformis; BOrMGS-2 and BOrMGS-3 as Bacillus pumilus; and BBoB2L2-2 as Bacillus sp.","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study of Philippine freshwater algae has primarily focused on microscopic and planktonic forms such as those of Velasquez (1962), Pantastico (1977), Tamayo-Zafaralla (1998) among others, with little information known about macroscopic forms. Among the larger, benthic forms inhabiting freshwater habitats, seven species in five genera of red algae (Rhodophyta) have so far been documented from the Philippines. Two of these species belong to the Batrachospermaceae as currently circumscribed by Entwisle et al. (2009), with one species Batrachospermum nonocense Kumano et Liao originally described from the Philippines, with its type locality in Nonoc Island, Surigao del Norte province. Another freshwater red alga, Nemalionopsis shawii Skuja, also has a Philippine type locality (Lamao Reserve, Bataan province) and is the generitype species of Nemalionopsis Skuja currently placed within the Thoreaceae, which was recently accommodated into its new segregate order, the Thoreales by Müller et al. (2002).
对菲律宾淡水藻类的研究主要集中在微观和浮游形式，如Velasquez (1962)， Pantastico (1977)， Tamayo-Zafaralla(1998)等，对宏观形式知之甚少。在生活在淡水栖息地的大型底栖生物中，迄今为止在菲律宾已记录到红藻(红藻门)5属7种。其中2种属于Entwisle et al.(2009)目前划定的batrachospermacae科，其中1种Batrachospermum nonocense Kumano et Liao最初描述于菲律宾，其类型地点位于北苏里高省Nonoc岛。另一种淡水红藻Nemalionopsis shawii Skuja也有一个菲律宾类型的地方(巴丹省Lamao保护区)，是Nemalionopsis Skuja的属型物种，目前被置于Thoreaceae中，最近被m ller等人(2002年)纳入其新的分离目Thoreales。
{"title":"Nomenclatural Notes on some Philippine Species of Freshwater Red Algae (Rhodophyta)","authors":"L. Liao","doi":"10.3860/PJSB.V4I0.1567","DOIUrl":"https://doi.org/10.3860/PJSB.V4I0.1567","url":null,"abstract":"The study of Philippine freshwater algae has primarily focused on microscopic and planktonic forms such as those of Velasquez (1962), Pantastico (1977), Tamayo-Zafaralla (1998) among others, with little information known about macroscopic forms. Among the larger, benthic forms inhabiting freshwater habitats, seven species in five genera of red algae (Rhodophyta) have so far been documented from the Philippines. Two of these species belong to the Batrachospermaceae as currently circumscribed by Entwisle et al. (2009), with one species Batrachospermum nonocense Kumano et Liao originally described from the Philippines, with its type locality in Nonoc Island, Surigao del Norte province. Another freshwater red alga, Nemalionopsis shawii Skuja, also has a Philippine type locality (Lamao Reserve, Bataan province) and is the generitype species of Nemalionopsis Skuja currently placed within the Thoreaceae, which was recently accommodated into its new segregate order, the Thoreales by Müller et al. (2002).","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The leaf architecture of Malvaceae sensu APG was examined and characterized to determine if it can be used in classification of the family and the identification of its species. Forty species were observed, measured and described. A dichotomous key was constructed based solely on leaf architecture characters. The dichotomous key indicated that leaf architecture characters can be used in distinguishing some species of Malvaceae sensu APG. Some basic leaf architectural characters can also be used in describing certain clades within the family. It is recommended that specimens are collected personally instead on relying on available specimens in the herbarium. Preparation of leaf skeletons through clearing method can also be done in future studies. Increase of sample size is also recommended.
本文对Malvaceae sensu APG的叶构型进行了研究和表征，以确定其是否可用于该科的分类和物种的鉴定。对40个物种进行了观察、测量和描述。基于叶结构特征构造了一个二分类键。二分类键表明，叶片结构特征可用于某些种类锦葵的分类。一些基本的叶结构特征也可以用来描述科内的某些分支。建议亲自收集标本，而不是依靠植物标本室的现有标本。在未来的研究中，还可以通过清除法制备叶骨架。还建议增加样本量。
{"title":"Leaf Architecture of Selected Species of Malvaceae sensu APF and its Taxonomic Significance","authors":"Allen Anthony P Laraño, I. Buot","doi":"10.3860/PJSB.V4I0.1563","DOIUrl":"https://doi.org/10.3860/PJSB.V4I0.1563","url":null,"abstract":"The leaf architecture of Malvaceae sensu APG was examined and characterized to determine if it can be used in classification of the family and the identification of its species. Forty species were observed, measured and described. A dichotomous key was constructed based solely on leaf architecture characters. The dichotomous key indicated that leaf architecture characters can be used in distinguishing some species of Malvaceae sensu APG. Some basic leaf architectural characters can also be used in describing certain clades within the family. It is recommended that specimens are collected personally instead on relying on available specimens in the herbarium. Preparation of leaf skeletons through clearing method can also be done in future studies. Increase of sample size is also recommended.","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Four new species of plants under the family Dipterocarpaceae from the Philippines are here described: Hopea reynosoi Gut., Rojo & Madulid; Hopea santosiana Gut., Rojo & Madulid, Shorea arsorianoi Gut., Rojo & Madulid and Vatica mendozae Gut., Rojo & Madulid
{"title":"New Dipterocarp Species from the Philippines","authors":"H. G. Gutierrez, J. P. Rojo, D. Madulid","doi":"10.3860/pjsb.v4i0.1565","DOIUrl":"https://doi.org/10.3860/pjsb.v4i0.1565","url":null,"abstract":"Four new species of plants under the family Dipterocarpaceae from the Philippines are here described: Hopea reynosoi Gut., Rojo & Madulid; Hopea santosiana Gut., Rojo & Madulid, Shorea arsorianoi Gut., Rojo & Madulid and Vatica mendozae Gut., Rojo & Madulid","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization and Identification of High Cellulase-Producing Bacterial Strains from Philippine Mangroves","authors":"Nik Shawn C. Tabao, R. Monsalud","doi":"10.3860/PJSB.V4I0.1562","DOIUrl":"https://doi.org/10.3860/PJSB.V4I0.1562","url":null,"abstract":"","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arleen L Buera, O. Laurente, Bismark Oliver C Lemana, G. D. Alejandro
The Philippine Marsdenieae (Apocynaceae-Asclepiadoideae) comprises a total of seven genera including the imperfectly known Clemensiella Schltr. Due to its unique morphology, the position of the small genus in the tribe Marsdenieae of Asclepiadoideae was previously in question. In this first molecular study of Clemensiella , the internal transcribed spacer (ITS, nrDNA) region of three isolates were newly generated and analyzed together with previous published sequences. The present aims to assess the phylogenetic positon, monophyly and closely-related genera of Clememsiella . The three Clemensiella isolates yielded a total of 630 to 642 base pairs (bp) and the average GC content ranged from 63.21% to 63.86%. Based on the strict consensus tree, the monophyly of Clemensiella is highly supported with BS = 100% and nested within the tribe Marsdenieae with BS = 61%. This study confirms the phylogenetic position of Clemensiella in Marsdenieae and its close relation to Dischidia and Hoya . Although the three Marsdenieae are distinct in their seed structures, they share common features in habit, inflorescence and corolla aestivation. KEYWORDS: Clemensiella ; ITS, Marsdenieae; Apocynaceae; Asclepiadoideae DOI: 10.3860/pjsb.v4i0.1564 P hilippine Journal of Systematic Biology Vol. IV (June 2010) 55-66
{"title":"Molecular Confirmation on the Phylogenetic Position of the Genus Clemensiella Schltr. in Marsdenieae (Apocynaceae - Asclepiadoideae)","authors":"Arleen L Buera, O. Laurente, Bismark Oliver C Lemana, G. D. Alejandro","doi":"10.3860/pjsb.v4i0.1564","DOIUrl":"https://doi.org/10.3860/pjsb.v4i0.1564","url":null,"abstract":"The Philippine Marsdenieae (Apocynaceae-Asclepiadoideae) comprises a total of seven genera including the imperfectly known Clemensiella Schltr. Due to its unique morphology, the position of the small genus in the tribe Marsdenieae of Asclepiadoideae was previously in question. In this first molecular study of Clemensiella , the internal transcribed spacer (ITS, nrDNA) region of three isolates were newly generated and analyzed together with previous published sequences. The present aims to assess the phylogenetic positon, monophyly and closely-related genera of Clememsiella . The three Clemensiella isolates yielded a total of 630 to 642 base pairs (bp) and the average GC content ranged from 63.21% to 63.86%. Based on the strict consensus tree, the monophyly of Clemensiella is highly supported with BS = 100% and nested within the tribe Marsdenieae with BS = 61%. This study confirms the phylogenetic position of Clemensiella in Marsdenieae and its close relation to Dischidia and Hoya . Although the three Marsdenieae are distinct in their seed structures, they share common features in habit, inflorescence and corolla aestivation. KEYWORDS: Clemensiella ; ITS, Marsdenieae; Apocynaceae; Asclepiadoideae DOI: 10.3860/pjsb.v4i0.1564 P hilippine Journal of Systematic Biology Vol. IV (June 2010) 55-66","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"4 1","pages":"55-66"},"PeriodicalIF":0.0,"publicationDate":"2010-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70068017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Ramirez, Connel O Go, Sophia Anglica S Hernandez, Hanah I Ruiz, M. Sabit, T. D. Cruz
Marine yeasts can be isolated from various substrata. Their potential industrial application merits a thorough study of these microorganisms. Thus, our research study aimed to isolate and characterize marine yeasts from several substrata collected from Calatagan, Batangas. Marine yeasts were isolated using enrichment culture of seawater, marine sediments, and living and decaying seagrasses and seaweeds on Glucose-Yeast Extract-Peptone broth (GYPS) supplemented with 33 g/L marine salts. Following incubation at room temperature, a total of 13 yeast strains were isolated. Most of the marine yeasts were obtained from either living or decaying seagrasses. Growth on GYP Broth with and without salt showed that six out of the 13 isolates were able to grow in the presence or absence of marine salts. Characterization of the isolated marine yeasts was done using the conventional morphological and biochemical methods and modern molecular techniques. All marine yeasts have cream-colored, circular and convex colonies with entire margin. The cells were spherical with a size range of 2.5 – 12.5 μm. They utilized galactose, maltose, sucrose, trehalose, xylose, sorbitol, N-acetyl-glucosamine and 2-keto-gluconate. Only one isolate (PCL08-LG04) utilized glycerol, arabinose, cellobiose, and raffinose. Comparison of the API 20C Aux profiles with the API database indicated that the isolated marine yeasts have affinity to the genus Candida. Gene sequence analysis of their ITS 1 and 2 regions of nuclear rDNA identified the isolates as Candida tropicalis and Pichia carribica.
海洋酵母可以从各种基质中分离出来。它们潜在的工业应用值得对这些微生物进行深入的研究。因此，我们的研究旨在从八打雁岛卡拉达干收集的几种基质中分离和表征海洋酵母。在添加了33 g/L海盐的葡萄糖-酵母提取物-蛋白胨肉汤(GYPS)上，对海水、海洋沉积物、活海草和腐烂海草和海藻进行富集培养，分离出海洋酵母。室温培养后，共分离出13株酵母菌株。大多数海洋酵母是从活的或腐烂的海草中获得的。在加盐和不加盐的GYP肉汤上的生长表明，13个分离株中有6个能够在存在或不存在海盐的情况下生长。采用常规形态学、生化方法和现代分子技术对分离的海洋酵母进行了鉴定。所有的海洋酵母都有奶油色，圆形和凸的菌落，整个边缘。细胞呈球形，尺寸范围为2.5 ~ 12.5 μm。他们使用了半乳糖、麦芽糖、蔗糖、海藻糖、木糖、山梨醇、n -乙酰氨基葡萄糖和2-酮葡萄糖酸盐。只有一个分离株(PCL08-LG04)利用了甘油、阿拉伯糖、纤维素糖和棉子糖。与API数据库的比较表明，分离得到的海洋酵母与念珠菌有亲缘关系。核rDNA ITS 1和ITS 2区基因序列分析鉴定为热带假丝酵母和加勒比毕赤酵母。
{"title":"Characterization of Marine Yeasts isolated from different Substrates Collected in Calatagan, Batangas","authors":"C. Ramirez, Connel O Go, Sophia Anglica S Hernandez, Hanah I Ruiz, M. Sabit, T. D. Cruz","doi":"10.3860/PJSB.V4I0.1561","DOIUrl":"https://doi.org/10.3860/PJSB.V4I0.1561","url":null,"abstract":"Marine yeasts can be isolated from various substrata. Their potential industrial application merits a thorough study of these microorganisms. Thus, our research study aimed to isolate and characterize marine yeasts from several substrata collected from Calatagan, Batangas. Marine yeasts were isolated using enrichment culture of seawater, marine sediments, and living and decaying seagrasses and seaweeds on Glucose-Yeast Extract-Peptone broth (GYPS) supplemented with 33 g/L marine salts. Following incubation at room temperature, a total of 13 yeast strains were isolated. Most of the marine yeasts were obtained from either living or decaying seagrasses. Growth on GYP Broth with and without salt showed that six out of the 13 isolates were able to grow in the presence or absence of marine salts. Characterization of the isolated marine yeasts was done using the conventional morphological and biochemical methods and modern molecular techniques. All marine yeasts have cream-colored, circular and convex colonies with entire margin. The cells were spherical with a size range of 2.5 – 12.5 μm. They utilized galactose, maltose, sucrose, trehalose, xylose, sorbitol, N-acetyl-glucosamine and 2-keto-gluconate. Only one isolate (PCL08-LG04) utilized glycerol, arabinose, cellobiose, and raffinose. Comparison of the API 20C Aux profiles with the API database indicated that the isolated marine yeasts have affinity to the genus Candida. Gene sequence analysis of their ITS 1 and 2 regions of nuclear rDNA identified the isolates as Candida tropicalis and Pichia carribica.","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. B. M. Buiser, Janel G Baliling, Robelle R Esguerra, Adrian G Galut, Sabrina D Oliveros, D. M. D. CRUZ-PAPA
{"title":"Two New Tailed Pseudomonad Bacteriophages from The Philippines","authors":"P. B. M. Buiser, Janel G Baliling, Robelle R Esguerra, Adrian G Galut, Sabrina D Oliveros, D. M. D. CRUZ-PAPA","doi":"10.3860/PJSB.V3I1.1011","DOIUrl":"https://doi.org/10.3860/PJSB.V3I1.1011","url":null,"abstract":"","PeriodicalId":37378,"journal":{"name":"Philippine Journal of Systematic Biology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70067642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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