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Diversity of agaricoid mushrooms in the Afromontane forests of Kedjom-Keku, North West Region, Cameroon 喀麦隆西北地区Kedjom-Keku非洲山地森林中类木脂蘑菇的多样性
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/15
FS Fungwa
A study of the diversity of gill mushroom was carried out in the primary and secondary forests of Kedjom-keku montane forest, North West region, Cameroon. The purpose of this study was to conduct a comparative study of the specific diversity of fungi collected in these two forest types. Out of the two hundred and fifteen samples collected, there were strictly twenty-five species in the primary forests, twenty-one species in the secondary forests and fourteen species were common to both forests. In the primary forest, the most represented genera were Mycena, Crepidotus, Pluteus, Crinipellis and Agaricus, and the most abundant families were Mycenaceae, Agaricaceae, Marasmiaceae, Inocybaceae, Psathyrellaceae, while, the most represented genera in the secondary forest were Mycena, Gymnopus, Agaricus, Pluteus, Crepidotus and the most abundant families were Mycenaceae, Agaricaceae, Marasmiaceae, Psathyrelaceae and Omphalotaceae. In terms of ecology, 64% of the species was collected on wood, 40% from soil, 8% on wood and soil, 1% on litter and wood. All species collected were saprotrophs. The trend of this initial study showed that the primary forest had a higher biodiversity of agaric fungi though not significantly different from that of the secondary forest. This makes this forest ideal for the conservation of macrofungi.
对喀麦隆西北地区Kedjom-keku山地森林的原生林和次生林中刺菇的多样性进行了研究。本研究的目的是对这两种森林类型中采集的真菌的具体多样性进行比较研究。在采集到的215份样本中,原生林中有25种,次生林中有21种,两林共有14种。在原生林中,最具代表性的属为Mycena、Crepidotus、Pluteus、Crinipellis和Agaricus,最丰富的科为Mycenaceae、Agaricaceae、Marasmiaceae、Inocybaceae、Psathyrelaceae,次生林中最具代表性的属为Mycena、Gymnopus、Agaricus、Pluteus、Crepidotus,最丰富的科为Mycenaceae、Agaricaceae、Marasmiaceae、Psathyrelaceae和Omphalotaceae。在生态学方面,64%的树种采集于木材,40%采集于土壤,8%采集于木材和土壤,1%采集于凋落物和木材。采集到的种类均为腐生菌。初步研究结果表明,原生林的木耳真菌多样性高于次生林,但与次生林的差异不显著。这使得这片森林成为保存大型真菌的理想场所。
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引用次数: 0
– Obtaining new hybrid strains of Agaricus bisporus by crossing homokaryons from wild Mexican and commercial strains -利用墨西哥野生菌株和商业菌株的同核体杂交获得双孢蘑菇新菌株
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/36
J. Chen
Breeding A. bisporus is complicated due to its two pseudohomothallic and heterothallic life cycles resulting from the production of both heterokaryotic and homokaryotic basidiospores, respectively. This makes it difficult to retrieve homokaryotic offspring required for outcrossing. Mexico has a long history of using wild edible mushrooms, and mushroom cultivation, especially A. bisporus, and these practices have steadily increased in recent decades. However, the production of A. bisporus still depends on foreign cultivars. Wild collections of A. bisporus were recently reported from Mexico. This study aimed to obtain new hybrid strains of A. bisporus by crossing homokaryons from these wild strains and the homokaryon U1-7 from the commercial strain U1. Single spore isolates (SSIs) were obtained from spore prints directly from cultivated sporocarps. Putative homokaryotic SSIs were first selected based on culture morphology and confirmed using a cleaved amplified polymorphic sequence (CAPS) marker in the gene mip. Selected homokaryotic SSIs and the homokaryotic strain U1-7 were crossed. Six putative hybrid strains were obtained and tested for fruiting. Their primordia appeared after 40 to 55 days. The variability was recorded for unit weight (5.3 to 44.6 g), pileus diameter (3.3 to 8.5 cm) and pileus firmness (5.01 to 8.77 N). These are the first hybrid strains of A. bisporus obtained by crossing wild strains from Mexico.
双孢酵母的繁殖是复杂的,因为它有两个假同壳和异壳的生命周期,分别由异核和同核的担子孢子产生。这使得获得异交所需的同核后代变得困难。墨西哥使用野生食用蘑菇和种植蘑菇,特别是双孢蘑菇的历史悠久,近几十年来这些做法稳步增加。然而,双孢菇的生产仍然依赖于国外品种。最近在墨西哥报道了双孢螨的野生采集。本研究旨在将这些野生菌株的同核体与商业菌株U1的同核体U1-7杂交,获得新的双孢霉杂交菌株。单孢子分离物(ssi)是直接从培养的孢子囊中获得的。首先根据培养形态选择推测的同核细胞ssi,并使用mip基因的cleaved amplified polymorphic sequence (CAPS)标记进行确认。将选择的同核ssi与同核菌株U1-7杂交。获得了6个推测杂交品系,并进行了结实试验。它们的原基在40至55天后出现。单株重量(5.3 ~ 44.6 g)、菌毛直径(3.3 ~ 8.5 cm)和菌毛硬度(5.01 ~ 8.77 N)均有变异。这是首次通过与墨西哥野生菌株杂交获得的双孢单胞菌杂交菌株。
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引用次数: 0
Conlarium indicum: A novel fungus from Western Ghats of India 印度西高止山脉的一种新型真菌
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/9
R. Dubey, S. Manikpuri
The present paper describes and illustrates Conlarium indicum, a new species in the family Conlariaceae (Ascomycota). This taxon was isolated as an epiphyte from decaying Bamboo collected from Western Ghats of India. The isolate was identified based on asexual-morphs, cultural characteristics and phylogenetic analyses of partial nuclear ribosomal 28S large subunit (LSU) and complete internal transcribed spacer (ITS) rDNA. Phylogenetic reconstructions based on ITS+LSU sequences show that the new species form independent monophyletic groups and are well separated from previously known seven species of Conlarium viz. C. dupliciascosporum (as duplumascospora), C. aquaticum, C. thailandense, C. sacchari, C. baiseense, C. nanningense and C. subglobosum. This supports the erection of C. indicum as a new species. A brief description of the morphology of C. indicum with morphological and molecular data is provided. To our understanding, the present taxon has turned out to be hitherto unreported.
本文报道了子囊菌科(Conlariaceae)的一新种——棘球蚴(Conlarium indicum)。本分类群是从印度西高止山脉的腐竹中分离出来的附生植物。根据部分核糖体28S大亚基(LSU)和完整的内部转录间隔段(ITS) rDNA的无性形态、培养特征和系统发育分析鉴定了该分离物。基于ITS+LSU序列的系统发育重建表明,新种形成了独立的单系群,与已知的7种冠状虫(c.a dupliciascosporum, as duplumascospora)、c.a aquaticum、c.t aiandense、c.c sacchari、c.b aisense、c.c nanningense和c.a subglobosum)有很好的分离。这支持了籼稻作为新种的建立。简要介绍了籼稻的形态和分子数据。据我们所知,目前的分类群是迄今未被报道过的。
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引用次数: 2
Aspergilli screened for antagonistic effect on fungal plant pathogens and for endoglucanase and xylanase activities 筛选曲霉对植物真菌病原菌的拮抗作用以及内切葡聚糖酶和木聚糖酶的活性
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/20
K. Tang
Aspergilli screened for antagonistic effect on fungal plant pathogens and for endoglucanase and xylanase activities. Abstract Twenty-six selected Aspergilli isolates were quantitatively pre-screened for endoglucanase and xylanase activities and for antagonistic effect on growth of plant pathogens using a dual plug inoculation method on agar plates. The Aspergilli were identified based on β-tubulin and calmodulin gene sequencing data using GenBank-based BLASTn analysis (NCBI). Antagonistic activity of the Aspergilli against fungal plant pathogens (i.e., Curvularia eragrostidis, Colletotrichum gloeosporioides , Neopestalotiopsis clavispora , Fusarium incanatum and Sclerotium rolfsii ) was measured in vitro after seven days of incubation at room temperature. The highest antagonistic Aspergilli against Curvularia eragrostidis , Colletotrichum gloeosporioides , Neopestalotiopsis clavispora , Fusarium incanatum and Sclerotium rolfsii were identified as Aspergillus carbonarius WP 2, A. parasiticus JNF 222, A. carbonarius WP 2, A. carbonarius TBRC 402 and A. giganteus TBRC 400, respectively. The highest enzymatic index (EI) of endoglucanase and xylanase activity found in Aspergillus puniceus TBRC 2711 and A. candidus TBRC 2770 were 2.92 and 2.88, respectively. In this study, the preliminary screening reveals the potential of Aspergilli isolates as sources of endoglucanase and xylanase activity and as effective biocontrol agents against plant pathogenic fungi.
筛选曲霉对植物真菌病原菌的拮抗作用以及内切葡聚糖酶和木聚糖酶的活性。摘要采用琼脂平板双插接种法,对26株曲霉进行了内切葡聚糖酶和木聚糖酶活性的定量预筛选,并对植物病原菌的生长进行了拮抗。利用基于genbank的BLASTn分析(NCBI)对β-微管蛋白和钙调蛋白基因测序数据进行鉴定。在室温条件下培养7天后,测定了曲霉对植物真菌病原菌(即曲霉、炭疽菌、新青芽孢菌、白镰刀菌和罗氏菌核菌)的拮抗活性。结果表明,对黑藤曲霉、炭疽炭疽菌、枯枝新青霉、白镰刀菌和罗氏菌核菌拮抗效果最好的曲霉分别为炭疽曲霉WP 2、寄生曲霉JNF 222、炭疽曲霉WP 2、炭疽曲霉tbrc402和巨曲曲霉tbrc400。梨曲霉TBRC 2711和假丝曲霉TBRC 2770的内切葡聚糖酶和木聚糖酶活性最高,分别为2.92和2.88。在本研究中,初步筛选揭示了曲霉分离物作为内切葡聚糖酶和木聚糖酶活性的来源和作为有效的植物病原真菌生物防治剂的潜力。
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引用次数: 0
Plant protection properties of the Plant GrowthPromoting Fungi (PGPF): Mechanisms and potentiality 植物生长促进真菌(PGPF)的植物保护特性:机制和潜力
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/29
SS El-Maraghy, AT Tohamy, K. Hussein
For the past few decades, arbuscular mycorrhizal fungi have been applied as effective inoculants to enhance plant growth and productivity. The rhizosphere-inhabiting microorganisms which result in improvement of growth and protection of plant can be collectively defined as plant growth promoting microorganisms (PGPM). Plant growth-promoting rhizobacteria (PGPR) improve plant growth and supports the plant to endure abiotic and biotic stresses. Plant growthpromoting fungi (PGPF) are known to colonize the region of the root of plants, and they enhance the plant nutrient uptake. The utilization of proper preparations of beneficial microorganisms is an inevitable demand to diminish the inputs of inorganic fertilizers, herbicides, and pesticides; moreover, it’s one of the most promising trends for sustainable agriculture and maintaining agricultural productivity. The production of strong inhibitory compounds by PGPF affects many plant pathogens. Fungi are omnipresent on organic compounds. The majority is saprobes and decomposes dead organic matter. Many excellent reviews about endophytic fungi in woody plants have already been published. However, only limited studies focused on the rhizosphere free-living fungi efficacy for plant growth promotion. This review will consider the mode of actions of these fungi to better understand the promising contributions of PGPF in enhancing plant growth and protection.
在过去的几十年里,丛枝菌根真菌作为一种有效的接种剂被应用于植物的生长和生产力。对植物生长起促进和保护作用的根际微生物统称为植物生长促进微生物(plant growth promoting microbial, PGPM)。促进植物生长的根瘤菌(PGPR)促进植物生长,支持植物承受非生物和生物胁迫。植物生长促进真菌(Plant growthpromoting fungi, PGPF)可以在植物根部定植,增强植物对养分的吸收。利用有益微生物的适当制剂是减少无机肥料、除草剂和杀虫剂投入的必然要求;此外,这是可持续农业和保持农业生产力最有希望的趋势之一。PGPF产生的强抑制化合物影响许多植物病原体。真菌在有机化合物中无所不在。大多数是腐殖菌和分解死亡的有机物。关于木本植物内生真菌的研究已发表了许多优秀的综述。然而,关于根际游离真菌促进植物生长的研究很少。本文将对这些真菌的作用模式进行综述,以更好地了解PGPF在促进植物生长和保护方面的潜在贡献。
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引用次数: 9
Evaluation of the activity of crude extracts from semi-arid soil fungi against clinical yeasts and molds 半干旱土壤真菌粗提物对临床酵母菌和霉菌的活性评价
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/7
Sofia Quintero Romero, R. Comerio, A. Romero, R. Vitale
In recent years, the incidence and severity of fungal diseases has increased, particularly in populations with a broad list of immunocompromised conditions. Moreover, the emergence of azole resistance is arising. For these reasons and the small number of available antifungal agents, searching for new and effective compounds with antifungal activity is mandatory. The aim of this study was to evaluate the antifungal activity of crude extracts from semi-arid soil fungi against strains of clinical molds and yeasts. For screening purposes crude extracts of fifteen isolates, corresponding to ten fungal species, were preliminarily explored against Candida albicans ATCC6414 by diffusion methodology. Special focus was placed on Aspergillus tatenoi, Leiothecium ellipsoideum, Subplenodomus violicola and Trichoderma saturnisporum extracts because of their preceding antifungal performance. Crude extracts of these species were once more examined and assayed against 54 yeast and 24 molds including the genera Aspergillus, Candida and Cryptococcus. Antifungal susceptibility testing by microdilution methodology was performed. Activity, as the percentage of clinical strains inhibited by different extract concentrations, ranged from 60.5% to 100% for A. tatenoi, L. ellipsoideum and T. saturnisporum extracts. On the other hand, S. violicola extract was active against all the strains tested with MIC values ≤ 0.25 μg/mL. This study dealt with active crude extracts; particularly, the extract from S. violicola has shown a potent and promising antifungal activity. The composition of the active fractions and the mechanisms of action involved remain to be studied and warrant further investigations.
近年来,真菌疾病的发病率和严重程度有所增加,特别是在免疫功能低下的人群中。此外,还出现了对唑的耐药性。由于这些原因和少量可用的抗真菌药物,寻找新的和有效的化合物具有抗真菌活性是必须的。本研究的目的是评价半干旱土壤真菌粗提物对临床霉菌和酵母菌的抑菌活性。采用扩散法对10个真菌种类的15株白色念珠菌ATCC6414粗提物进行初步筛选。由于之前的抗真菌性能,我们特别关注了黑曲霉、椭圆状Leiothecium ellipsoideum、violendous violicola和saturnisporum木霉的提取物。这些物种的粗提取物再次被检测并测定了54种酵母和24种霉菌,包括曲霉属、念珠菌属和隐球菌属。采用微量稀释法进行抗真菌药敏试验。结果表明,不同浓度提取物对三种病原菌的抑制率在60.5% ~ 100%之间。另一方面,堇菜提取物对MIC值≤0.25 μg/mL的所有菌株均有活性。本研究涉及活性粗提物;其中,堇菜提取物具有较强的抗真菌活性。活性组分的组成和所涉及的作用机制仍有待研究,值得进一步研究。
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引用次数: 0
Morphological and growth characteristics of Clathrus ruber P.Micheli ex Pers. vegetative mycelium in vitro condition 橡胶树的形态和生长特征。体外条件下的营养菌丝体
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/25
M. Sukhomlyn, N. Tsvyd, I. Poplevicheva, M. Pasailiuk
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引用次数: 0
Review of the basic cultivation conditions influence on the growth of basidiomycetes 基本培养条件对担子菌生长的影响
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/34
T. Krupodorova, VYu Barshteyn, AS Sekan
Recently, the significant economic and therapeutic potential of different fungi causes the intense cultivation of the prospective species. This review presents the analysis of the basic physicochemical conditions for fungi cultivation that have an influence on basidiomycetes’ mycelia or biomass growth options (maximal mycelial dry weight, maximal mycelial growth, maximal mycelial growth rate, mycelial density, colony diameter, etc.). Not only different species, but different strains of the same fungus require different cultivation conditions, the variability of nutrition media, temperature, pH, carbon and nitrogen sources. Several parameters optimal cultivation can be explained by the geographical and climatic factors. Analysis of literature data and the resulting conclusions will help a deeper understanding of the biological characteristics of fungi, their nutritional needs, optimal temperature and pH, and optimization of the production of fungal biomass. Comparison of the collected information on various fungal strains growth aimed to facilitate the further development and optimization in existing cultivation protocols. According to this, the maximum growth for biotechnological and industrial application is expected to be obtained. Also, the prolongation in safekeeping of viable fungal forms and the preservation of the fungi biodiversity for their re-introduction in nature is expected as one of the benefits after protocol optimization.
近年来,不同真菌的显著经济和治疗潜力引起了对潜在物种的强烈培养。本文综述了影响担子菌菌丝或生物量生长选择(最大菌丝干重、最大菌丝生长、最大菌丝生长速率、菌丝密度、菌落直径等)的真菌培养基本理化条件。同一菌种的不同菌种需要不同的培养条件、不同的营养介质、温度、pH、碳氮源。地理和气候因素可以解释最佳栽培的几个参数。对文献数据的分析和得出的结论将有助于更深入地了解真菌的生物学特性、营养需求、最佳温度和pH,以及真菌生物量的优化生产。对收集到的各种真菌菌株生长信息进行比较,旨在促进现有培养方案的进一步发展和优化。据此,预计生物技术和工业应用将获得最大的增长。此外,方案优化后的好处之一是延长了真菌存活形式的保存时间,并保存了真菌的生物多样性,使其能够重新引入自然界。
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引用次数: 3
New records of Amanita citrinoannulata and A. pakistanica (Amanitaceae) from India 标题印度黄毒伞属和巴基斯坦毒伞属新记录
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/23
T. Mehmood
Amanita citrinoannulata A. (Amanitaceae) Abstract Amanita citrinoannulata and A. pakistanica are reported here as new records for India. Detailed morphological descriptions supported by line drawings, color photo plates and a phylogram based on nrLSU are provided for these two species.
摘要本文报道了在印度新记录的黄毒伞(Amanita citrinoannulata)和巴基斯坦毒伞(A. pakistan)。通过线条图、彩色照片板和基于nrLSU的系统分布图对这两个物种进行了详细的形态描述。
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引用次数: 0
Biodegradation of plastics waste using fungi: A review 利用真菌降解塑料废弃物的研究进展
Q3 Environmental Science Pub Date : 2021-01-01 DOI: 10.5943/cream/11/1/1
AP Asiandu, A. Wahyudi, S. Sari
Plastics are synthetic polymers that are widely used in every field of life every day. Along with the increasing use of plastic, the amount of plastic waste produced and accumulated to environment will also increase. If the plastic waste is not handled properly, it will pollute the environment and threaten many living things including humans. Biodegradation is a promising method for dealing with plastic waste. This method includes many microbes including fungi such as Aspergillus spp. and Penicillium spp. as its biodegradation agents. Some plastics degrading fungi produce many specific enzymes that catalyze the degradation of plastic polymer into simpler and smaller fragments including oligomer, dimer, and monomer through several steps including biodeterioration, depolymerization, assimilation, and mineralization. The fragmented plastic particles are absorbed and used by plastic degrading fungi as their energy and carbon sources. Biodegradation is the appropriate method to overcome the plastics pollution because this method has no side effects as the conventional methods.
塑料是一种合成聚合物,广泛应用于日常生活的各个领域。随着塑料使用量的增加,产生和积累到环境中的塑料废物的数量也将增加。如果塑料垃圾处理不当,它会污染环境,威胁到包括人类在内的许多生物。生物降解是一种很有前途的处理塑料垃圾的方法。该方法包括许多微生物,包括真菌,如曲霉和青霉菌作为其生物降解剂。一些塑料降解真菌产生许多特定的酶,通过生物降解、解聚、同化和矿化等几个步骤,催化塑料聚合物降解成更简单和更小的片段,包括低聚物、二聚体和单体。破碎的塑料颗粒被塑料降解真菌吸收和利用,作为它们的能量和碳源。生物降解是克服塑料污染的合适方法,因为它与传统方法相比没有副作用。
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引用次数: 2
期刊
Current Research in Environmental and Applied Mycology
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