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Multiscale modeling of molecule transport through skin’s deeper layers 分子通过皮肤深层传输的多尺度模型
Pub Date : 2023-05-01 DOI: 10.1016/j.comtox.2023.100267
Nitu Verma , Kishore Gajula , Rakesh Gupta , Beena Rai

Accurate in-silico models of human skin are required to obtain the uptake/release of molecules across the skin layers to supplement the in-vivo/in-vitro experiments for faster development/testing of cosmetics and drugs. We aim to develop an in-silico skin permeation model by extending the multiscale modeling framework developed earlier for skin’s top layer to deeper layer and compared the outcomes with in-vitro experimental permeation data of 43 cosmetic-relevant molecules across human skin.

In this study, we have extended a multiscale modeling framework, with realistic heterogeneous stratum corneum (SC) comprising of network of permeable lipids and corneocytes, followed by homogeneous viable epidermis and dermis. The diffusion coefficients of molecules in lipid layer were determined using molecular dynamics simulations, whereas the diffusion coefficients in other layers and all the partition coefficients were calculated from correlations reported in literature. These parameters were then used in the macroscopic models to predict the release profiles of drugs through the deeper skin layers. The obtained release profiles were in good agreement with available experimental data for most of the molecules. The reported model could provide insight into cosmetics/drugs skin permeation and act as a time-saving and efficient guiding tool for performing targeted experiments.

需要精确的人体皮肤硅模型来获得分子在皮肤层上的摄取/释放,以补充体内/体外实验,从而更快地开发/测试化妆品和药物。我们的目标是通过将先前开发的皮肤表层多尺度建模框架扩展到更深的皮肤层,建立一个硅皮肤渗透模型,并将结果与43种化妆品相关分子在人体皮肤中的体外实验渗透数据进行比较。在这项研究中,我们扩展了一个多尺度的建模框架,用真实的非均质角质层(SC),包括可渗透的脂质和角质层网络,其次是均匀的活表皮和真皮层。脂质层分子的扩散系数采用分子动力学模拟方法确定,其他层的扩散系数和所有分配系数采用文献报道的相关性计算。然后将这些参数用于宏观模型,以预测药物通过更深的皮肤层的释放曲线。所获得的释放曲线与大多数分子的现有实验数据一致。该模型可以深入了解化妆品/药物的皮肤渗透,并作为进行针对性实验的省时高效的指导工具。
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引用次数: 1
Macitentan: An overview of its degradation products, process-related impurities, and in silico toxicity. Macitentan:概述其降解产物,工艺相关杂质和硅毒性
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2022.100255
Eduardo Costa Pinto, Luana Gonçalves de Souza, Carolina Trajano Velozo, Gil Mendes Viana, Lucio Mendes Cabral, Valeria Pereira de Sousa

Macitentan is a dual endothelin receptor antagonist indicated for the treatment of pulmonary arterial hypertension, a chronic and complex disease. Under different stress conditions, such as changes in pH and temperature, the drug can generate a large number of degradation products, while many process-related impurities can occur during the four main synthetic routes. The assessment of the potential toxicity of these impurities is an essential regulatory requirement for the quality and safety of drugs. The goal of this study was to identify all metabolites and potential impurities for macitentan and evaluate their in silico toxicity. Thirty-five compounds related to macitentan were found reported in the literature, two of which were described simultaneously as metabolites, degradation products, and process-related impurities. In the present study, the main degradation products and the conditions under which they could be formed, and the major impurities according to the synthetic route, are discussed. The types and amounts of process-related impurities were dependent on the synthesis route and process controls, while macitentan was found to be more susceptible to degradation in acidic media resulting in the most different types of degradation products. The structure of each compound was generated and the potential risk for mutagenicity and carcinogenicity were determined using three different in silico platforms, in addition the metabolic substrate/inhibition profile for each compound was assessed. Overall, five compounds were considered critical as they had a possible toxicity risk in terms of mutagenicity, tumorigenicity, irritation, and reproductive effects. These data support the current legislation for raw materials and pharmaceutical products containing macitentan as to prevent any adverse effects from this drug.

马西坦是一种双重内皮素受体拮抗剂,用于治疗肺动脉高压这一慢性复杂疾病。在不同的应激条件下,如pH和温度的变化,药物可以产生大量的降解产物,而在四种主要合成路线中会产生许多与工艺相关的杂质。对这些杂质的潜在毒性进行评估是药品质量和安全的基本监管要求。本研究的目的是鉴定马西坦的所有代谢物和潜在杂质,并评估其硅毒性。文献中发现了35种与macitentan相关的化合物,其中两种同时被描述为代谢物、降解产物和工艺相关杂质。在本研究中,讨论了主要的降解产物及其形成的条件,以及合成路线中主要的杂质。与工艺相关的杂质的类型和数量取决于合成路线和工艺控制,而macitentan被发现更容易在酸性介质中降解,从而产生最不同类型的降解产物。生成了每种化合物的结构,并使用三种不同的硅平台确定了潜在的致突变性和致癌性风险,此外还评估了每种化合物的代谢底物/抑制谱。总的来说,五种化合物被认为是关键的,因为它们在致突变性、致瘤性、刺激性和生殖效应方面可能具有毒性风险。这些数据支持目前关于含有马西坦的原料和医药产品的立法,以防止这种药物的任何不良影响。
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引用次数: 2
Molecular recognition of some novel mTOR kinase inhibitors to develop anticancer leads by drug-likeness, molecular docking and molecular dynamics based virtual screening strategy 基于药物相似性、分子对接和分子动力学的虚拟筛选策略对一些新型mTOR激酶抑制剂的分子识别以开发抗癌线索
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2022.100257
Arka Das , Gurubasavaraja Swamy Purawarga Matada , Prasad Sanjay Dhiwar , Nulgumnalli Manjunathaiah Raghavendra , Nahid Abbas , Ekta Singh , Abhishek Ghara , Ganesh Prasad Shenoy

Cancer is the second leading cause of death worldwide. Among various anticancer drug targets, mTOR is noteworthy. Numerous first-generation mTOR inhibitors are already approved and few second-generation mTOR inhibitors targeting the kinase domain are in the clinical trials, but yet to reach the market, and many lead to serious toxicities. Here we are focused to discover some novel kinase inhibitors from the ZINC database which may effectively inhibit mTOR kinase. For this, computational chemistry and pharmacophore-based ZINC database search has been adopted. Series of virtual screening analysis lead to the discovery of 5 active hits. Among these 5, compound 4 (ZINC79476038) having binding energy of −8.9 Kcal/mol shows maximum interactions within the binding pocket. Study proved that all these compounds can potentially inhibit mTOR kinase and can be successfully developed as anticancer agents. We further proved that these compounds are not only active for general cancers like lung, breast, colon, and other peripheral cancers but also equally active in CNS, targeting numerous brain cancers.

癌症是全球第二大死因。在众多的抗癌药物靶点中,mTOR是值得关注的。许多第一代mTOR抑制剂已经被批准,针对激酶结构域的第二代mTOR抑制剂很少进入临床试验,但尚未进入市场,并且许多会导致严重的毒性。在这里,我们将重点从锌数据库中发现一些可能有效抑制mTOR激酶的新型激酶抑制剂。为此,采用了基于计算化学和药效团的ZINC数据库检索方法。通过一系列的虚拟筛选分析,发现了5个活跃点。其中,结合能为−8.9 Kcal/mol的化合物4 (ZINC79476038)在结合袋内的相互作用最大。研究证明,这些化合物都有潜在的抑制mTOR激酶的作用,可以成功地开发为抗癌药物。我们进一步证明,这些化合物不仅对肺癌、乳腺癌、结肠癌和其他外周癌症等普通癌症有效,而且对中枢神经系统也同样有效,针对多种脑癌。
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引用次数: 2
Development of a CSRML version of the Analog identification Methodology (AIM) fragments and their evaluation within the Generalised Read-Across (GenRA) approach 模拟识别方法(AIM)片段的CSRML版本的开发及其在泛化跨读(GenRA)方法中的评估
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2022.100256
Matthew Adams , Hannah Hidle , Daniel Chang , Ann M. Richard , Antony J. Williams , Imran Shah , Grace Patlewicz

The Analog Identification Methodology (AIM) was developed over 20 years ago to identify analogues to support read-across at the US Environmental Protection Agency. However, the current public version of the standalone tool, released in 2012, is no longer usable on Windows operating systems supported by Microsoft. Additionally, the structural logic for analogue selection is based on older, customised Simplified molecular-input-line-entry system (SMILES)-type features that are incompatible with modern cheminformatics tools. Given these limitations, a case study was undertaken to explore a more transparent, extensible method of implementing the AIM fragments using Chemical Subgraphs and Reactions Mark-up Language (CSRML). A CSRML file was developed to codify the original AIM fragments, and the extent to which AIM fragments were faithfully replicated was assessed using the AIM Database. The overall mean performance of the CSRML-AIM across all fragments in terms of sensitivity, specificity, and Jaccard similarity was 89.5%, 99.9%, and 82.2%, respectively. Comparing the AIM fragments with public ToxPrints using a large set of ∼25,000 substances of regulatory interest to EPA found them to be dissimilar, with an average maximum Jaccard score of 0.24 for AIM and 0.29 for ToxPrint fingerprints. Both fragment sets were then used as inputs in the automated read-across approach, Generalised Read-Across (GenRA), to evaluate the quality of fit in predicting rat acute oral toxicity LD50 values with the coefficient of determination (R2) and root mean squared error (RMSE). The performance of AIM fragments was R2=0.434 and RMSE=0.663 whereas that of ToxPrints was R2=0.477 and RMSE=0.638. A bootstrap resampling using 100 iterations found the mean and the 95th confidence interval of R2 to be 0.349 [0.319, 0.379] for AIM fragments and 0.377 [0.338, 0.412] for ToxPrints. Although AIM and ToxPrints performed similarly in predicting LD50, they differed in their performance at a local level, revealing that their features can offer complementary insights.

模拟物识别方法(AIM)是在20多年前开发的,用于识别类似物,以支持美国环境保护署的读取。但是,该独立工具的当前公开版本(2012年发布)已无法在微软支持的Windows操作系统上使用。此外,模拟物选择的结构逻辑是基于旧的,定制的简化分子输入行输入系统(SMILES)类型的特征,与现代化学信息学工具不兼容。考虑到这些限制,我们进行了一个案例研究,探索一种使用化学子图和反应标记语言(CSRML)实现AIM片段的更透明、可扩展的方法。开发了一个CSRML文件来对原始AIM片段进行编码,并使用AIM数据库评估AIM片段被忠实复制的程度。CSRML-AIM在所有片段的敏感性、特异性和Jaccard相似性方面的总体平均表现分别为89.5%、99.9%和82.2%。将AIM片段与公共ToxPrints进行比较,使用大量的约25,000种对EPA具有监管意义的物质,发现它们是不同的,AIM和ToxPrint指纹的平均最大Jaccard分数分别为0.24和0.29。然后将这两个片段集用作自动读取方法的输入,即广义读取(GenRA),以确定系数(R2)和均方根误差(RMSE)评估预测大鼠急性口服毒性LD50值的拟合质量。AIM片段的检测效能R2=0.434, RMSE=0.663, ToxPrints的检测效能R2=0.477, RMSE=0.638。使用100次迭代的bootstrap重采样发现,AIM片段的R2均值和第95可信区间为0.349 [0.319,0.379],ToxPrints的R2均值和可信区间为0.377[0.338,0.412]。尽管AIM和ToxPrints在预测LD50方面表现相似,但它们在局部水平上的表现不同,这表明它们的特征可以提供互补的见解。
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引用次数: 1
A review of quantitative structure-activity relationship modelling approaches to predict the toxicity of mixtures 预测混合物毒性的定量构效关系建模方法综述
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2022.100251
Samuel J. Belfield , James W. Firman , Steven J. Enoch , Judith C. Madden , Knut Erik Tollefsen , Mark T.D. Cronin

Exposure to chemicals generally occurs in the form of mixtures. However, the great majority of the toxicity data, upon which chemical safety decisions are based, relate only to single compounds. It is currently unfeasible to test a fully representative proportion of mixtures for potential harmful effects and, as such, in silico modelling provides a practical solution to inform safety assessment. Traditional methodologies for deriving estimations of mixture effects, exemplified by principles such as concentration addition (CA) and independent action (IA), are limited as regards the scope of chemical combinations to which they can reliably be applied. Development of appropriate quantitative structure-activity relationships (QSARs) has been put forward as a solution to the shortcomings present within these techniques – allowing for the potential formulation of versatile predictive tools capable of capturing the activities of a full contingent of possible mixtures. This review addresses the current state-of-the-art as regards application of QSAR towards mixture toxicity, discussing the challenges inherent in the task, whilst considering the strengths and limitations of existing approaches. Forty studies are examined within – through reference to several characteristic elements including the nature of the chemicals and endpoints modelled, the form of descriptors adopted, and the principles behind the statistical techniques employed. Recommendations are in turn provided for practices which may assist in further advancing the field, most notably with regards to ensuring confidence in the acquired predictions.

接触化学物质通常以混合物的形式发生。然而,化学安全决策所依据的绝大多数毒性数据仅与单一化合物有关。目前还不可能对完全具有代表性的混合物比例进行潜在有害影响的测试,因此,计算机模拟为安全评估提供了一种实用的解决方案。以浓度加法(CA)和独立作用(IA)等原理为例,用于估计混合效应的传统方法在它们可以可靠地应用于化学组合的范围方面是有限的。提出了适当的定量结构-活性关系(qsar)的发展,作为这些技术中存在的缺点的解决方案-允许潜在的多功能预测工具的制定,能够捕获所有可能混合物的活性。本文综述了QSAR在混合毒性方面的应用,讨论了任务中固有的挑战,同时考虑了现有方法的优势和局限性。通过参考几个特征元素,包括化学物质的性质和建模的端点,所采用的描述符的形式以及所采用的统计技术背后的原则,对40项研究进行了检查。然后又为可能有助于进一步推进这一领域的做法提出建议,特别是在确保对所获得的预测的信心方面。
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引用次数: 4
Exploring genetic influences on adverse outcome pathways using heuristic simulation and graph data science 使用启发式模拟和图形数据科学探索遗传对不良结果途径的影响。
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2023.100261
Joseph D. Romano , Liang Mei , Jonathan Senn , Jason H. Moore , Holly M. Mortensen

Adverse outcome pathways provide a powerful tool for understanding the biological signaling cascades that lead to disease outcomes following toxicity. The framework outlines downstream responses known as key events, culminating in a clinically significant adverse outcome as a final result of the toxic exposure. Here we use the AOP framework combined with artificial intelligence methods to gain novel insights into genetic mechanisms that underlie toxicity-mediated adverse health outcomes. Specifically, we focus on liver cancer as a case study with diverse underlying mechanisms that are clinically significant. Our approach uses two complementary AI techniques: Generative modeling via automated machine learning and genetic algorithms, and graph machine learning. We used data from the US Environmental Protection Agency’s Adverse Outcome Pathway Database (AOP-DB; aopdb.epa.gov) and the UK Biobank’s genetic data repository. We use the AOP-DB to extract disease-specific AOPs and build graph neural networks used in our final analyses. We use the UK Biobank to retrieve real-world genotype and phenotype data, where genotypes are based on single nucleotide polymorphism data extracted from the AOP-DB, and phenotypes are case/control cohorts for the disease of interest (liver cancer) corresponding to those adverse outcome pathways. We also use propensity score matching to appropriately sample based on important covariates (demographics, comorbidities, and social deprivation indices) and to balance the case and control populations in our machine language training/testing datasets. Finally, we describe a novel putative risk factor for LC that depends on genetic variation in both the aryl-hydrocarbon receptor (AHR) and ATP binding cassette subfamily B member 11 (ABCB11) genes.

不良结果通路为理解毒性后导致疾病结果的生物信号级联提供了一个强大的工具。该框架概述了被称为关键事件的下游反应,最终导致毒性暴露的临床显著不良结果。在这里,我们将AOP框架与人工智能方法相结合,以获得对毒性介导的不良健康结果背后的遗传机制的新见解。具体而言,我们将重点放在癌症作为一个具有不同潜在机制的案例研究,这些机制具有临床意义。我们的方法使用了两种互补的人工智能技术:通过自动机器学习和遗传算法的生成建模,以及图形机器学习。我们使用了来自美国环境保护局不良反应途径数据库(AOP-DB;aopdb.epa.gov)和英国生物银行基因数据库的数据。我们使用AOP-DB来提取疾病特异性AOP,并构建用于最终分析的图神经网络。我们使用英国生物库检索真实世界的基因型和表型数据,其中基因型基于从AOP-DB中提取的单核苷酸多态性数据,表型是与这些不良结果途径相对应的感兴趣疾病(癌症)的病例/对照组。我们还使用倾向得分匹配来根据重要的协变量(人口统计、合并症和社会剥夺指数)进行适当的抽样,并在我们的机器语言训练/测试数据集中平衡病例和对照人群。最后,我们描述了一种新的LC推定风险因子,该因子依赖于芳香烃受体(AHR)和ATP结合盒亚家族B成员11(ABCB11)基因的遗传变异。
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引用次数: 1
ToxicR: A computational platform in R for computational toxicology and dose–response analyses 毒物R:一个用于计算毒理学和剂量反应分析的R语言计算平台
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2022.100259
Matthew W. Wheeler , Sooyeong Lim , John S. House , Keith R. Shockley , A. John Bailer , Jennifer Fostel , Longlong Yang , Dawan Talley , Ashwin Raghuraman , Jeffery S. Gift , J. Allen Davis , Scott S. Auerbach , Alison A. Motsinger-Reif

The need to analyze the complex relationships observed in high-throughput toxicogenomic and other omic platforms has resulted in an explosion of methodological advances in computational toxicology. However, advancements in the literature often outpace the development of software researchers can implement in their pipelines, and existing software is frequently based on pre-specified workflows built from well-vetted assumptions that may not be optimal for novel research questions. Accordingly, there is a need for a stable platform and open-source codebase attached to a programming language that allows users to program new algorithms. To fill this gap, the Biostatistics and Computational Biology Branch of the National Institute of Environmental Health Sciences, in cooperation with the National Toxicology Program (NTP) and US Environmental Protection Agency (EPA), developed ToxicR, an open-source R programming package. The ToxicR platform implements many of the standard analyses used by the NTP and EPA, including dose–response analyses for continuous and dichotomous data that employ Bayesian, maximum likelihood, and model averaging methods, as well as many standard tests the NTP uses in rodent toxicology and carcinogenicity studies, such as the poly-K and Jonckheere trend tests. ToxicR is built on the same codebase as current versions of the EPA’s Benchmark Dose software and NTP’s BMDExpress software but has increased flexibility because it directly accesses this software. To demonstrate ToxicR, we developed a custom workflow to illustrate its capabilities for analyzing toxicogenomic data. The unique features of ToxicR will allow researchers in other fields to add modules, increasing its functionality in the future.

分析在高通量毒代基因组和其他组学平台中观察到的复杂关系的需要导致了计算毒理学方法学的爆炸式发展。然而,文献中的进步往往超过了研究人员可以在其管道中实现的软件的开发,现有软件通常基于预先指定的工作流程,这些工作流程是根据经过充分审查的假设构建的,可能不适合新的研究问题。因此,需要一个稳定的平台和连接到编程语言的开源代码库,允许用户对新算法进行编程。为了填补这一空白,美国国家环境健康科学研究所生物统计学和计算生物学分会与国家毒理学计划(NTP)和美国环境保护局(EPA)合作,开发了ToxicR,一个开源的R编程包。ToxicR平台实现了NTP和EPA使用的许多标准分析,包括使用贝叶斯、最大似然和模型平均方法对连续和二分数据进行的剂量-反应分析,以及NTP在啮齿动物毒理学和致癌性研究中使用的许多标准测试,如poly-K和Jonckheere趋势测试。ToxicR与EPA的Benchmark Dose软件和NTP的BMDExpress软件的当前版本建立在相同的代码库上,但由于它直接访问该软件,因此增加了灵活性。为了演示ToxicR,我们开发了一个自定义工作流程来说明其分析毒代基因组数据的能力。ToxicR的独特功能将允许其他领域的研究人员添加模块,从而在未来增加其功能。
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引用次数: 1
Benzoxazole based thiazole hybrid analogs: Synthesis, in vitro cholinesterase inhibition, and molecular docking studies 苯并恶唑基噻唑杂化类似物:合成、体外胆碱酯酶抑制及分子对接研究
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2022.100253
Rafaqat Hussain , Fazal Rahim , Wajid Rehman , Syed Adnan Ali Shah , Shoaib Khan , Imran Khan , Liaqat Rasheed , Syahrul Imran , Abdul Wadood , Magda H. Abdellatif

Acetylcholinesterase and butyrylcholinesterase enzymes are therapeutic target for Alzheimer disease and their inhibitors play a vital role for the treatment of this disease. A new series of benzoxazole based 1,3-thiazole hybrid scaffolds (120) were synthesized and assessed for acetylcholinesterase and butyrylcholinesterase inhibition profile and then characterized by using different spectroscopic tools such as 1H NMR, 13C NMR and HREI-MS spectroscopy. Four scaffolds such as 1, 4, 12 and 19 showed AChE potency almost comparable to standard drug having IC50 values 0.692 ± 0.087, 0.947 ± 0.089, 0.38 ± 0.016 and 0.742 ± 0.042 µM, while nine scaffolds such as 1, 4, 6, 8, 9, 12, 13, 14 and 19 showed superior BuChE potency than standard drug having IC50 values 2.54 ± 0.10, 1.79 ± 0.20, 3.25 ± 0.18, 2.48 ± 0.05, 1.33 ± 0.05, 2.19 ± 0.08, 2.81 ± 0.20, 2.23 ± 0.10 and 2.10 ± 0.05 µM respectively. Nonetheless, remaining analogs were found to have moderate activity. Among the synthesized series, analogs 12 (IC50 = 0.38 ± 0.016 µM) and 9 (IC50 = 1.33 ± 0.05 µM) were identified as the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase enzymes. In addition, the molecular docking studies were carried out to find out the possible binding mode of interactions of most active analogs with enzymes active site and results supported the experimental data.

乙酰胆碱酯酶和丁基胆碱酯酶是阿尔茨海默病的治疗靶点,其抑制剂在阿尔茨海默病的治疗中起着至关重要的作用。合成了一系列新的以苯并恶唑为基础的1,3-噻唑杂化支架(1 - 20),对其乙酰胆碱酯酶和丁基胆碱酯酶的抑制性能进行了评价,并利用1H NMR、13C NMR和HREI-MS等不同的波谱工具对其进行了表征。四个支架,如1、4、12和19显示疼痛效果几乎与标准药物IC50值0.692±0.087,0.947±0.089,0.38±0.016,0.742±0.042µM,而九个支架,如1,4,6,8,9日,12日,13日,14日和19显示优越的大餐比标准药物效力IC50值2.54±0.10,1.79±0.20,3.25±0.18,2.48±0.05,1.33±0.05,2.19±0.08,2.81±0.20,2.23±0.10,2.10±0.05µM分别。尽管如此,剩下的类似物被发现有适度的活性。在所合成的系列化合物中,类似物12 (IC50 = 0.38±0.016µM)和9 (IC50 = 1.33±0.05µM)是乙酰胆碱酯酶和丁基胆碱酯酶最有效的抑制剂。此外,我们还进行了分子对接研究,找出了大多数活性类似物与酶活性位点的相互作用可能的结合方式,结果支持了实验数据。
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引用次数: 4
Safety assessment of the use of recycled high-density polyethylene in cosmetics packaging based on in silico modeling migration of representative chemical contaminants for dermal sensitization and systemic endpoints 化妆品包装中使用再生高密度聚乙烯的安全性评估基于典型化学污染物的皮肤致敏和系统终点的硅模拟迁移
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2023.100260
Ramez Labib , Ripal Amin , Christal Lewis , Valer Toşa , Peter Mercea

A safety assessment of recycled high-density polyethylene (rHDPE) in cosmetic packaging was performed based on guidelines published by the European Food Safety Authority (EFSA) on the use of recycled plastics for food packaging. EFSA guidelines require demonstration that the concentration of selected representative chemical contaminants in recycled plastic resulting from exposure from food is lower than the threshold of toxicological concern (TTC) for genotoxic substances of 0.0025 µg/kg bw/day. To investigate the highest concentration (Cmod) of representative chemical contaminants, that would not exceed the genotoxic TTC, when migrating from rHDPE packaging to foodstuffs, used as cosmetic formulation surrogates, we used mathematical modeling software (MIGRATEST®EXP). The Cmod values of representative chemical contaminants were then compared with the EFSA-reported residual concentration (Cres) of each contaminant in the rHDPE. For each of the cosmetic product/packaging combinations evaluated, we found that the modeled values were clearly lower for Cmod than Cres, i.e., the recycling process could effectively reduce potential contaminants of rHDPE to levels that would not result in daily consumer exposure from cosmetic use exceeding the genotoxic TTC. For skin sensitization, we modeled a worst-case scenario and assumed 100 % of each representative chemical contaminant migrates into the cosmetic formulation from rHDPE. We then calculated the consumer exposure level for each contaminant based on the dose per unit area and compared it with the dermal sensitization threshold (DST) for reactive materials, which is 64 µg/cm2. In each case, we demonstrated that the migration of each representative chemical contaminant from rHDPE into each cosmetic formulation was far below the DST, confirming that there is no appreciable risk of sensitization for protein-reactive chemicals. In conclusion, these data support the safe use of rHDPE in the packaging of cosmetic products for leave-on and rinse-off applications.

根据欧洲食品安全局(EFSA)发布的关于在食品包装中使用再生塑料的指导方针,对化妆品包装中的再生高密度聚乙烯(rHDPE)进行了安全评估。欧洲食品安全局的指导方针要求证明,因接触食品而产生的再生塑料中选定的代表性化学污染物的浓度低于基因毒性物质的毒理学关注阈值(TTC) 0.0025微克/千克体重/天。为了研究作为化妆品配方替代品从rHDPE包装迁移到食品时不超过遗传毒性TTC的代表性化学污染物的最高浓度(Cmod),我们使用了数学建模软件(MIGRATEST®EXP)。然后将代表性化学污染物的Cmod值与欧洲食品安全局报告的rHDPE中每种污染物的残留浓度(Cres)进行比较。对于评估的每种化妆品/包装组合,我们发现Cmod的模型值明显低于Cres,即回收过程可以有效地将rHDPE的潜在污染物降低到不会导致消费者每日接触化妆品超过遗传毒性TTC的水平。对于皮肤致敏,我们模拟了最坏的情况,并假设每种代表性化学污染物100%从rHDPE迁移到化妆品配方中。然后,我们根据每单位面积的剂量计算了每种污染物的消费者暴露水平,并将其与活性物质的皮肤致敏阈值(DST)(64µg/cm2)进行了比较。在每种情况下,我们都证明了从rHDPE到每种化妆品配方的每种代表性化学污染物的迁移量远低于DST,证实了蛋白质反应性化学物质没有明显的致敏风险。总之,这些数据支持在化妆品包装中安全使用rHDPE,用于免洗和冲洗应用。
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引用次数: 0
In silico prediction of persistent, mobile, and toxic pharmaceuticals (PMT): A case study in São Paulo Metropolitan Region, Brazil 持久性、流动性和毒性药物(PMT)的计算机预测:巴西圣保罗大都会区的案例研究
Pub Date : 2023-02-01 DOI: 10.1016/j.comtox.2022.100254
Vinicius Roveri , Luciana Lopes Guimarães

Computational modelling (in silico) methods based on quantitative structure-activity relationship ((Q)SAR) models, are powerful tools for the assessment of the potential “persistency, mobility, and toxicity” (PMT) of pharmaceuticals compounds. Moreover, the use of (Q)SAR models, is recommended by European Union’s REACH Regulation. In this context, the aims of this research were estimating, for the first time and based by REACH guidelines, the PMT potentials of 115 most sold pharmaceuticals in São Paulo Metropolitan Region (a megacity with 21 million of Brazilian), through five (Q)SAR updated models, namely: the OPERA QSAR; the VEGA QSAR (Version 1.1.5); the EPI Suite (Version 4.11); the ECOSAR (Version, 2.0); and the QSAR Toolbox (Version 4.5). This study prioritized the in-silico predictions from the OPERA and the VEGA, because both QSARs can generate reliable predictions, i.e., they have detailed information about the applicability domains. In silico predictions were performed considering ten endpoints: (i) Molecular weight (MW); (ii) “STP total removal”: Sewage Treatment Plant; (iii) Octanol-water partition coefficient (KOW); (iv) Predicted ready biodegradability; (v) Soil organic adsorption coefficient (KOC); (vi) “Short-term and long-term ecological assessments”; (vii) “Carcinogenicity”; (viii) “Mutagenicity”; (ix) “Estrogen receptor binding”; (x) “Cramer decision tree”. The main results showed that: (a) These 115 pharmaceuticals cover a wide range of so-called small molecules (range from 100 to 600 MW); (b) In STP, a predicted removal lower than 10 % was found for 76 pharmaceuticals; (c) Additionally, 101 chemicals has low (Log KOW <2.5), or medium sorption potential (2.5< log KOW <4.0); (d) Ultimately, 36 PPCPs were considered “persistent” after a weight-of-evidence assessment. In addiction, 17 among these 36 persistent chemicals, were classified as “very mobile” in water (log KOC <3). Finally, only three among 17 PPCPs, namely ciprofibrate, fluconazole and metoclopramide, exhibited one or more toxic characteristics (described in items vi – x). These results it will be possible to alert about the potential risks arising from the indiscriminate disposal of these PPCPs along the water sources of this Brazilian mega metropolis.

基于定量构效关系((Q)SAR)模型的计算建模(计算机)方法是评估药物化合物潜在的“持久性、移动性和毒性”(PMT)的有力工具。此外,欧盟REACH法规建议使用(Q)SAR模型。在此背景下,本研究的目的是首次根据REACH指南,通过五个(Q)SAR更新模型,估计圣保罗大都市区(一个拥有2100万巴西人的大城市)115种最畅销药物的PMT潜力,即:OPERA QSAR;VEGA QSAR (Version 1.1.5);EPI套件(4.11版);西非经委会(2.0版);和QSAR工具箱(版本4.5)。本研究优先考虑来自OPERA和VEGA的计算机预测,因为这两个qsar都可以生成可靠的预测,即它们具有有关适用领域的详细信息。计算机预测考虑了十个终点:(i)分子量(MW);(ii)“全面拆除STP”:污水处理厂;(iii)辛醇-水分配系数(KOW);预测的现成生物降解性;(v)土壤有机吸附系数;“短期和长期生态评价”;(七)“致癌性”;(八)“诱变”;(ix)“雌激素受体结合”;(x)“克莱默决策树”。主要结果表明:(a)这115种药物涵盖了广泛的所谓小分子(范围从100到600 MW);(b)在STP中,76种药物的预测去除率低于10%;(c)此外,101种化学物质具有低(Log KOW <2.5)或中等吸附电位(2.5<log kw <4.0);(d)最终,36个ppcp在证据权重评估后被认为是“持久的”。在成瘾性方面,这36种持久性化学物质中有17种在水中被归类为“非常流动”(log KOC <3)。最后,在17种ppcp中,只有三种,即环丙贝特、氟康唑和甲氧氯普胺,表现出一种或多种毒性特征(见第vi - x项)。这些结果将有可能提醒人们,在这个巴西大都市的水源附近随意处置这些ppcp会产生潜在风险。
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引用次数: 4
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Computational Toxicology
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