Journal of Clinical Tuberculosis and Other Mycobacterial Diseases最新文献

{"title":"Mycobacterium tuberculosis infection status and associated factors among household close contacts of rifampicin-resistant pulmonary tuberculosis patients: A single-center cross-sectional study","authors":"Zhengyu Shi , Juan Peng , Xiu Li , Xiaoyan Fu , Liping Zou , Qin Chen , Tao Huang , Yu Zhou , Hongmei Zhu , Yi Wang , Shenjie Tang , Guihui Wu","doi":"10.1016/j.jctube.2025.100561","DOIUrl":"10.1016/j.jctube.2025.100561","url":null,"abstract":"<div><h3>Background</h3><div>Rifampicin-resistant tuberculosis (RR-TB) is a major global public health challenge. Household close contacts (HHCs) of RR-TB patients face a high risk of <em>Mycobacterium tuberculosis</em> infection. Southwestern China carries a heavy RR-TB burden, yet research data on the infection status of this population remain scarce. This study aimed to evaluate the incidence of latent tuberculosis infection (LTBI) and active tuberculosis disease (TBD) and their associated factors among HHCs of RR-TB patients in this region through active screening, providing data support for optimizing regional RR-TB prevention and control strategies.</div></div><div><h3>Methods</h3><div>Using a cross-sectional design, HHCs of RR-TB patients diagnosed at Chengdu Public Health Clinical Medical Center from October 1, 2023, to March 30, 2025, were enrolled. Clinical data were collected via a self-designed questionnaire including gender, age, body mass index (BMI), relationship to index case, living environment, chronic comorbidities, medication history, and TBD-suspicious symptom screening. TBD screening used chest digital radiography (DR) or computed tomography (CT). Clinical data of index cases were extracted from the hospital information system (HIS), covering sputum acid-fast bacillus smear, sputum mycobacterial culture, sputum molecular testing for M. tuberculosis, fluoroquinolone resistance, extent of pulmonary lesions, and cavitation status. TB infection screening employed tuberculin skin test (TST), ESAT6-CFP10 fusion protein skin test (EC), or interferon-γ release assays (IGRA). Infection status was categorized as uninfected, LTBI, or TBD based on screening results, clinical symptoms, and imaging findings. Incidence rates of LTBI and TBD were calculated. Chi-square tests compared clinical characteristics across infection states. Multivariable logistic regression analyzed factors associated with LTBI and TBD (versus uninfected).</div></div><div><h3>Results</h3><div>264 HHCs from 197 RR-TB index cases were included: 113 males (42.8 %), 151 females (57.2 %), aged 3–78 years (mean 42.4 ± 15.1). Among 209 participants tested with TST: 117 (44.3 %) had induration diameter [0, 5) mm, 17 (6.4 %) [5, 10) mm, 30 (11.4 %) [10, 15) mm, 45 (17.1 %) ≥ 15 mm. Among 46 EC-tested: 29 (11.0 %) had [0, 5) mm, 17 (6.4 %) ≥ 5 mm. Among 9 IGRA-tested: 1 (0.4 %) negative, 8 (3.0 %) positive. After cluster-effect adjustment, LTBI incidence was 31.2 % (95 % confidence interval [CI]: 25.8–38.3), TBD incidence 9.9 % (95 % CI: 6.4–13.6). The proportion of spousal relationships to index cases was higher in LTBI/TBD groups than uninfected (P < 0.05). BMI < 18.5 kg/m<sup>2</sup> and positive TBD symptom screening were more frequent in TBD than uninfected/LTBI groups (P < 0.05). Pulmonary cavitation in index cases was more common in TBD contacts (P < 0.05). Multivariable analysis showed spousal relationship was an independent associated factor for LTBI (ad","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"41 ","pages":"Article 100561"},"PeriodicalIF":2.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145104935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting the therapeutic threshold in tuberculosis Care: Lessons from Southern Africa’s dual-method approach","authors":"Chadaporn N. Gordon , Nav La , Schawanya K. Rattanapitoon , Nathkapach K. Rattanapitoon","doi":"10.1016/j.jctube.2025.100560","DOIUrl":"10.1016/j.jctube.2025.100560","url":null,"abstract":"","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"41 ","pages":"Article 100560"},"PeriodicalIF":2.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of nontuberculous Mycobacteria among presumptive drug resistance tuberculosis patients from a ministry of health drug resistance surveillance program, in western Kenya","authors":"Okumu Albert ,&nbsp;Odeny Lazarus ,&nbsp;Ochieng John Benjamin ,&nbsp;Odhiambo Ben ,&nbsp;Sitati Ruth ,&nbsp;Tonui Joan ,&nbsp;Ogoro Jeremiah ,&nbsp;Wandiga Steve ,&nbsp;Collins Ouma","doi":"10.1016/j.jctube.2025.100559","DOIUrl":"10.1016/j.jctube.2025.100559","url":null,"abstract":"<div><h3>Introduction</h3><div>Nontuberculous Mycobacteria (NTM) species are emerging pathogens causing Pulmonary diseases with no definitive treatment. Molecular techniques enable characterization and drug resistance profiling, this study sought to determine NTM prevalence, circulating species, and distribution factors among presumptive multidrug-resistant tuberculosis (MDR-TB) patients in western Kenya.</div></div><div><h3>Method</h3><div>Sputum samples were collected between March through October 2022, and transported for testing at Kenya Medical Research Institute (KEMRI) TB laboratory, in Kisumu. The standard NALC-NaOH MGIT culture technique, smear, HAIN AS/CM and NTM drug resistance were carried out.</div></div><div><h3>Results</h3><div>Of the 155 specimens analyzed, 106 (68.4 %) were males, 41 (26.5 %) HIV positive, and participants of ages 36–45 years, the majority. An overall NTM prevalence of 99 (63.9 %), of whom 63 (63 %) among males reported. In addition, 11 NTM species identified, with <em>M. intracellulare</em> (44, 44 %).</div></div><div><h3>Conclusions</h3><div>High prevalence of NTM species was observed among middle-aged males and HIV negative participants, Kisumu led in distribution (29 %) and among HIV positive. The NTM prevalence among smear negative vs smear positive, was significant a p &lt; 0.001, hence adequate TB/HIV integration and management, use of molecular techniques, and accurate identification is critical.</div></div>","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"41 ","pages":"Article 100559"},"PeriodicalIF":2.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term occupational risk of latent tuberculosis infection in Hamburg, Germany: Findings from a 13-year prospective observational study","authors":"Roland Diel ,&nbsp;Matthias Gröschel ,&nbsp;Albert Nienhaus","doi":"10.1016/j.jctube.2025.100558","DOIUrl":"10.1016/j.jctube.2025.100558","url":null,"abstract":"<div><h3>Background</h3><div>Only limited population-based data are available on the risk of latent tuberculosis infection (LTBI) in health care workers (HCWs).</div></div><div><h3>Objective</h3><div>To assess the long-term effects of protective measures of HCWs on LTBI risk in Hamburg, Germany.</div></div><div><h3>Methods</h3><div>Close contacts of smear-positive and smear-negative, but culture-confirmed, pulmonary TB index cases were prospectively enrolled from June 2005 to December 2017 and tested with the QuantiFERON TB (QFT) test approximately eight weeks after last exposure<strong>.</strong> Sociodemographic and clinical data were collected by trained healthcare personnel using a standardized questionnaire.</div><div>Contacts with known previous positive TST or IGRA results were excluded.</div></div><div><h3>Results</h3><div>After exclusion of prevalent TB cases and contact persons who had been tested positive in other settings, valid results were available for 937 index cases and 6980 close contacts (average per case 7.45; standard deviation (SD) ± 9.99; range 1–83). Of the contacts, 3459 (49.6 %) were males and 3520 (50.4 %) females. 771 contacts (11.05 %) belonged to 11 HCW subgroups, most of them (475, or 62.8 %) hospital or geriatric nurses. Foreign-born HCW did not differ significantly from non-HCW regarding origin from high-incidence countries.</div><div>By adjusting for confounders, logistic regression analysis confirmed household contact as strongest predictor for acquiring LTBI (OR 3.8, p &lt; 0.001), followed by foreign-born status (OR 2.2, p &lt; 0.001) and male gender (OR 1.28, p &lt; 0.001). Contact with a smear-positive index case only slightly increased the risk of IGRA positivity, by 16 % (OR 1.16, p = 0.024). For each additional year of age, higher odds were found at 1.86 % (OR 1.019, p &lt; 0.001] and for each additional hour of contact at approximately 0.11 % (OR 1.011, p &lt; 0.001). BCG vaccination had no significant effect on IGRA test results (OR 0.95, p = 0.41).</div><div>Employment in healthcare overall was associated with a 26 % lower risk of IGRA positivity compared to non-HCWs (OR 0.74, p = 0.013); however, in a second adjusted model focusing on specific HCW subgroups, this risk reduction was statistically significant only for hospital and geriatric nurses, with no significant difference observed in other HCW subgroups.</div></div><div><h3>Conclusion</h3><div>Working in a health-care facility overall was associated with a lower LTBI risk compared to other risk factors<del>.</del> These findings suggest that protective measures might be particularly effective in hospital and geriatric nursing, while no risk reduction was evident for other HCW subgroups. Continued targeted protective measures remain important in high-risk care environments and support the relevance of recommendations issued (and last updated 2023) by the German Central Committee against Tuberculosis (DZK).</div></div>","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"41 ","pages":"Article 100558"},"PeriodicalIF":2.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symptomatic (STB) and Asymptomatic (ATB) tuberculosis in Italy: Results from a multicenter retrospective study","authors":"Sergio Cotugno ,&nbsp;Giacomo Guido ,&nbsp;Francesco Di Gennaro ,&nbsp;Francesco Cavallin ,&nbsp;Mariantonietta Pisaturo ,&nbsp;Lorenzo Onorato ,&nbsp;Federica Zimmerhofer ,&nbsp;Luca Pipitò ,&nbsp;Giuseppina De Iaco ,&nbsp;Giuseppe Bruno ,&nbsp;Massimo Fasano ,&nbsp;Agostina Pontarelli ,&nbsp;Annarita Botta ,&nbsp;Tiziana Iacovazzi ,&nbsp;Rossana Lattanzio ,&nbsp;Roberta Papagni ,&nbsp;Elda De Vita ,&nbsp;Alberto Zolezzi ,&nbsp;Gianfranco Panico ,&nbsp;Raffaella Libertone ,&nbsp;Annalisa Saracino","doi":"10.1016/j.jctube.2025.100556","DOIUrl":"10.1016/j.jctube.2025.100556","url":null,"abstract":"<div><h3>Objective</h3><div>Asymptomatic tuberculosis (ATB) represents a significant proportion of tuberculosis (TB) cases. This study aimed to compare ATB and symptomatic TB (STB) cases in terms of demographic and clinical characteristics, adverse events, hospital length of stay, and treatment outcomes.</div></div><div><h3>Methods</h3><div>This multicenter retrospective study included 510 microbiologically confirmed pulmonary TB patients across ten Italian hospitals between 2018 and 2023. STB cases presented with at least one symptom such as cough, fever, chest pain, hemoptysis, dyspnea, night sweats or weight loss. ATB cases presented with no symptoms. The endpoints included adverse events, length of hospital stay, and incompleteness of the treatment.</div></div><div><h3>Results</h3><div>ATB accounted for 36.4 % of cases (184/510). STB was significantly associated with diabetes (p = 0.03), hepatitis B/C infections (p &lt; 0.0001), and history of TB (p = 0.01). Adjusting for clinically relevant confounders, STB was associated with higher occurrence of adverse events (odds ratio 2.04, 95 % confidence interval 1.31 to 3.23; p = 0.002), more severe adverse events (odds ratio 8.07, 95 % confidence interval 2.58 to 33.34; p = 0.001) and a 24 % increase in length of hospital stay (95 % confidence interval 7 % to 47 %; p = 0.005), but was not associated with incomplete treatment (odds ratio 0.79, 95 % confidence interval 0.47 to 1.32; p = 0.37).</div></div><div><h3>Conclusions</h3><div>STB is associated with a higher burden of adverse events. ATB poses challenges for TB elimination due to its asymptomatic nature.</div></div>","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"41 ","pages":"Article 100556"},"PeriodicalIF":2.0,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving tuberculosis infection treatment completion among pregnant and postpartum women","authors":"Kristen A. Wendorf ,&nbsp;Lisa Armitige","doi":"10.1016/j.jctube.2025.100543","DOIUrl":"10.1016/j.jctube.2025.100543","url":null,"abstract":"","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"40 ","pages":"Article 100543"},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuberculosis infection control in MDR-TB designated hospitals in Jiangsu Province, China","authors":"Honghuan Song ,&nbsp;Guoli Li ,&nbsp;Zhuping Xu ,&nbsp;Feixian Wang ,&nbsp;Xiaoping Wang ,&nbsp;Bing Dai ,&nbsp;Xing Zhang ,&nbsp;Jincheng Li ,&nbsp;Yan Li ,&nbsp;Limei Zhu","doi":"10.1016/j.jctube.2025.100555","DOIUrl":"10.1016/j.jctube.2025.100555","url":null,"abstract":"<div><h3>Background</h3><div>Hospital-acquired Tuberculosis (TB) infections among healthcare workers (HCWs) and patients present a significant challenge due to the increased risk of TB infection within healthcare settings.</div></div><div><h3>Methods</h3><div>A standardized assessment tool was applied for the evaluation, which involved direct observation, document review, and interviews with facility heads. A baseline evaluation of TB infection control (TBIC) measures in TB outpatient and inpatient departments, as well as laboratories, was completed by January 2019. Based on the results, a comprehensive intervention package was implemented, incorporating a three-tiered hierarchy of controls: administrative control (AC), environmental control (EC), and respiratory protection (RP). Subsequent monitoring was conducted quarterly, with corrective actions accordingly. More than two years of follow-up data were collected, with the collaboration of local hospitals, the municipality Centers for Disease Control and Prevention (CDC), and the Jiangsu Provincial CDC, concluding on August 31, 2021.</div></div><div><h3>Results</h3><div>At baseline, the average implementation rates of AC, EC and RP were 57.3 %, 59.2 %, and 66.6 %, respectively. After the intervention, significant improvements were observed in key infection control measures. A triage process for cough patients was established, mechanical ventilation systems were installed, and the use of masks was improved. In addition, ultraviolet (UV) and upper-room ultraviolet germicidal irradiation (UVGI) systems were installed where required. As a result, the average implementation rates of AC, EC and RP significantly increased to 86.3 %, 87.4 %, and 98.4 % (P &lt; 0.05), respectively. However, at the study’s conclusion, Suzhou Fifth People’s Hospital reported a lower AC implementation rate of 70.7 %, while Changzhou Third People’s Hospital had an EC implementation rate of 68.1 %. These discrepancies were primarily attributed to suboptimal architectural designs that hindered proper ventilation in the wards.</div></div><div><h3>Conclusions</h3><div>This study demonstrates that designated hospitals still face persistent gaps in tuberculosis infection control (TBIC). However, over the course of one and a half years of targeted and standardized interventions, substantial improvements in TBIC practices were achieved across most participating institutions. Despite the suboptimal availability of dedicated TB wards, strengthening TBIC measures remains crucial to reducing TB transmission among healthcare workers and non-TB patients. This approach is both practical and scalable, particularly in high-burden TB settings. Nevertheless, the long-term efficacy and sustainability of these TBIC practices warrant ongoing evaluation.</div></div>","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"41 ","pages":"Article 100555"},"PeriodicalIF":2.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of nontuberculous mycobacterial infections in Saudi children from a tertiary care hospital perspective","authors":"Norah Abukhalid ,&nbsp;Nabeel Alzahrani ,&nbsp;Khaled Alsager ,&nbsp;Banan Alsowailmi ,&nbsp;Amjad Albawardi","doi":"10.1016/j.jctube.2025.100554","DOIUrl":"10.1016/j.jctube.2025.100554","url":null,"abstract":"<div><div>We present a case series of six children who were infected with different species of<!--> <!-->Nontuberculous mycobacteria (NTM) and <em>Mycobacterium riyadhense</em> was the most prevalent isolate representing 50% of the total pathogens. Four of the reported cases were immunocompromised with disseminated NTM diseases and two were infected with <em>M. avium</em>, <em>M. abscessus</em>, and the other two infected with <em>M. riyadhense</em>. Most patients responded to medical therapy, except for the <em>M. avium</em> case, which was fatal despite combination therapy. Due to the presence of mycolic acid in the cell wall of NTM isolates, prolonged combination therapy is required for treatment, and in some cases, natural resistance may also emerge<del>.</del> Most of the patients reported in our study were immunocompetent. This suggests that NTM can infect children at various body sites regardless of immune status. We highlighted our experience in diagnosing and treating these patients, with special attention to <em>M. riyadhense</em>.</div></div>","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"41 ","pages":"Article 100554"},"PeriodicalIF":2.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144826945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective analysis of systemic lupus erythematosus patients with latent tuberculosis infection: A 5-year follow-up study","authors":"Yiling Zhang ,&nbsp;Gengmin Zhou ,&nbsp;Hongli Wang ,&nbsp;Qingwen Wang ,&nbsp;Guofang Deng","doi":"10.1016/j.jctube.2025.100553","DOIUrl":"10.1016/j.jctube.2025.100553","url":null,"abstract":"<div><div>Patients with systemic lupus erythematosus (SLE) have a higher incidence of tuberculosis (TB)infection compared to the general healthy population. The use of glucocorticoids and immunosuppressive agents for SLE management further elevates TB risk. This study aimed to evaluate the prevalence of latent tuberculosis infection (LTBI) in SLE patients and explore risk factors for progression to active TB (ATB) in those with concurrent SLE and LTBI. We conducted a retrospective analysis of SLE patients treated at the Department of Rheumatology and Immunology, Peking University Shenzhen Hospital, between 2014 and 2023. During a five − year follow − up period, LTBI was detected in 122 patients (24.11%). Of these, 11 individuals (all from the subgroup of 108 patients who did not receive tuberculosis preventive treatment [TPT]) progressed to ATB. A comparative analysis between the 11 ATB cases and 111 non-progressing LTBI patients revealed significant differences: ATB cases showed higher cyclophosphamide (CTX) usage, elevated high-sensitivity C-reactive protein (hs-CRP) levels, and less frequent hydroxychloroquine (HCQ)administration. These findings underscore the need for regular monitoring during prolonged CTX therapy, especially in moderate-to-high TB burden regions, and highlight the potential protective role of HCQ.</div></div>","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"40 ","pages":"Article 100553"},"PeriodicalIF":1.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience","authors":"Senjuti Kabir ,&nbsp;Sabrina Choudhury ,&nbsp;Mohammad Khaja Mafij Uddin ,&nbsp;M.D. Fahim Ather ,&nbsp;Syed Mohammad Mazidur Rahman ,&nbsp;Pronab Kumar Modak ,&nbsp;Mohammad Samsuddoha Sarker Shanchay ,&nbsp;Tanjina Rahman ,&nbsp;Shahriar Ahmed ,&nbsp;Sayera Banu","doi":"10.1016/j.jctube.2025.100552","DOIUrl":"10.1016/j.jctube.2025.100552","url":null,"abstract":"<div><h3>Background</h3><div>Performance of OMNIgene.SPUTUM (OM-S) for transporting sputum was evaluated.</div></div><div><h3>Methods</h3><div>This exploratory study was conducted during January-December 2019 at four near and one distant healthcare-facilities of Dhaka. Smear-positive pulmonary TB patients’ sputa were collected, divided into ‘OM-S untreated’ and ‘OM-S treated’ portions, and transported to testing laboratory, Dhaka, on same-day from near-sites, and through courier from distant-site for smear-microscopy, culture, and Xpert MTB/RIF (Xpert) testing. Subset of ‘OM-S treated’ sample was tested with Xpert without centrifugation. Test results of all portions were compared in between.</div></div><div><h3>Results</h3><div>Total 444 participants were enrolled (near-sites:198, distant-site: 246). All test results were comparable in both portions for near-sites. For distant-site, smear-microscopy’s positivity was reduced by 4.1 % in ‘OM-S treated’, Xpert showed 100 % concordance in both portions, and culture was higher in ‘OM-S treated’ than ‘OM-S untreated’ (92.3 % vs 89.4 %; p = 0.288). Primary contamination rate in ‘OM-S treated’ was lower than ‘OM-S untreated’ (2.0 % vs 9.8 %; p &lt; 0.05). For all sites, median (IQR) time-to-culture positivity was 35 (28, 42) days in both portions. Xpert positivity was 99 % concordant in ‘OM-S treated’ regardless of centrifugation.</div></div><div><h3>Conclusions</h3><div>OM-S is safe for sputum transportation. OM-S mixed sputum can be tested with Xpert and culture. Further studies can validate findings and assess cost-effectiveness.</div></div>","PeriodicalId":37942,"journal":{"name":"Journal of Clinical Tuberculosis and Other Mycobacterial Diseases","volume":"40 ","pages":"Article 100552"},"PeriodicalIF":1.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}