Pub Date : 2022-12-30DOI: 10.59708/ajlhts.v1i4.2222
J. Etura, Kenneth.Igbor Ebi, U. Akpan, E. Asemota, A. O. Emeribe
Introduction: The welder’s flame contains toxic and mutagenic compounds that pose a potential health hazard associated with the occupation. This study assessed some haematological parameters in a population of welders in Calabar, Southern Nigeria. Methods: This was a cross-sectional study involving 50 welders exposed to oxyacetylene as test subjects and 50 non-welders as controls. The haematological parameters were analyzed using the Sysmex XE- 2100 haematology autoanalyser. Data was analyzed using SPSS version 21.0 with Student’s t-test and Pearson’s correlation. P <0.05 was considered statistically significant. Results: The platelets count was significantly lower (p=0.041) in the welders compared to the controls while neutrophils count was significantly higher (p=0.0001) in the welders than in the controls. The neutrophils count was significantly lower (p=0.0001) in welders exposed for 1-5 years compared to those exposed for over 5 years. Red blood cell count correlated negatively with platelets count (r= -0.415, p =0.003) while MCHC and MCV correlated negatively (r= -0.428, p =0.002) Conclusions: The study revealed a significant reduction and increase in platelets and neutrophils counts respectively in the welders exposed to oxyacetylene gas
导言:焊工的火焰含有有毒和致突变化合物，对职业健康有潜在危害。本研究评估了尼日利亚南部卡拉巴尔焊工人群的一些血液学参数。方法:这是一项横断面研究，涉及50名接触氧乙炔的焊工作为测试对象，50名非焊工作为对照。血液学参数分析采用Sysmex XE- 2100血液学自动分析仪。数据分析采用SPSS 21.0版，采用Student 's t检验和Pearson相关检验。P <0.05为差异有统计学意义。结果:焊工血小板计数显著低于对照组(p=0.041)，中性粒细胞计数显著高于对照组(p=0.0001)。与接触超过5年的焊工相比，接触1-5年的焊工中性粒细胞计数显著降低(p=0.0001)。红细胞计数与血小板计数呈负相关(r= -0.415, p =0.003)， MCHC和MCV呈负相关(r= -0.428, p =0.002)结论:暴露于氧乙炔气体的焊工血小板和中性粒细胞计数分别显著减少和增加
{"title":"Some haematological parameters of welders exposed to oxyacetylene in Calabar, Nigeria.","authors":"J. Etura, Kenneth.Igbor Ebi, U. Akpan, E. Asemota, A. O. Emeribe","doi":"10.59708/ajlhts.v1i4.2222","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i4.2222","url":null,"abstract":"Introduction: The welder’s flame contains toxic and mutagenic compounds that pose a potential health hazard associated with the occupation. This study assessed some haematological parameters in a population of welders in Calabar, Southern Nigeria. Methods: This was a cross-sectional study involving 50 welders exposed to oxyacetylene as test subjects and 50 non-welders as controls. The haematological parameters were analyzed using the Sysmex XE- 2100 haematology autoanalyser. Data was analyzed using SPSS version 21.0 with Student’s t-test and Pearson’s correlation. P <0.05 was considered statistically significant. Results: The platelets count was significantly lower (p=0.041) in the welders compared to the controls while neutrophils count was significantly higher (p=0.0001) in the welders than in the controls. The neutrophils count was significantly lower (p=0.0001) in welders exposed for 1-5 years compared to those exposed for over 5 years. Red blood cell count correlated negatively with platelets count (r= -0.415, p =0.003) while MCHC and MCV correlated negatively (r= -0.428, p =0.002) Conclusions: The study revealed a significant reduction and increase in platelets and neutrophils counts respectively in the welders exposed to oxyacetylene gas","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"229 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133988773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.59708/ajlhts.v1i4.2220
Yemisi Ogundare, M. A. Okungbowa, G. Ogundare, A. Esan, Fasakin Kolawole
Sickle cell anaemia is one of the life threatening health challenges bedevilling many developing countries like Nigeria and the management requires regular monitoring of the patients. The aim of this study was to determine haematological parameters and inflammatory markers among paediatrics with sickle cell anaemia. A total of 90 subjects were recruited for this study comprising of 45 confirmed sickle cell anaemia patients in steady state, 32 males and 13 females and 45 apparently healthy subjects with haemoglobin genotypes AA were used as the controls. Automated blood analyzer was used to analyze haematological parameters, erythrocyte sedimentation rate was estimated by Westergreen method and C-reactive protein was estimated using Enzyme Linked Immuno-Sorbent method. Total white blood cell count, white cell differential count, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, red cell distribution width, platelet count, platelet distribution width, erythrocyte sedimentation rate, C-reactive protein was higher in case compared to control. However, red blood cell count, haemoglobin, haematocrit, plateletcrit and mean platelet volume were lower in the case compared to the control subjects. There is no significant (p>0.05) difference in haematological parameters, erythrocyte sedimentation rate and C-reactive protein among the gender in both the case and the control except plateletcrit in the case and eosinophil and red cell distribution width in the control. There is similarity in haematocrit in both sex among the case. It is our anticipation that these findings will greatly assist physicians in the better management of Sickle cell patients in our locality.
{"title":"Haematological And Inflammatory Markers Among Paediatrics With Sickle Cell Anaemia Attending Tertiary Health Institutions in Ekiti State, Nigeria.","authors":"Yemisi Ogundare, M. A. Okungbowa, G. Ogundare, A. Esan, Fasakin Kolawole","doi":"10.59708/ajlhts.v1i4.2220","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i4.2220","url":null,"abstract":"Sickle cell anaemia is one of the life threatening health challenges bedevilling many developing countries like Nigeria and the management requires regular monitoring of the patients. The aim of this study was to determine haematological parameters and inflammatory markers among paediatrics with sickle cell anaemia. A total of 90 subjects were recruited for this study comprising of 45 confirmed sickle cell anaemia patients in steady state, 32 males and 13 females and 45 apparently healthy subjects with haemoglobin genotypes AA were used as the controls. Automated blood analyzer was used to analyze haematological parameters, erythrocyte sedimentation rate was estimated by Westergreen method and C-reactive protein was estimated using Enzyme Linked Immuno-Sorbent method. Total white blood cell count, white cell differential count, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, red cell distribution width, platelet count, platelet distribution width, erythrocyte sedimentation rate, C-reactive protein was higher in case compared to control. However, red blood cell count, haemoglobin, haematocrit, plateletcrit and mean platelet volume were lower in the case compared to the control subjects. There is no significant (p>0.05) difference in haematological parameters, erythrocyte sedimentation rate and C-reactive protein among the gender in both the case and the control except plateletcrit in the case and eosinophil and red cell distribution width in the control. There is similarity in haematocrit in both sex among the case. It is our anticipation that these findings will greatly assist physicians in the better management of Sickle cell patients in our locality.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125923199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.59708/ajlhts.v1i2.2219
I. Okafor, G. Anagha, Uchech Henshaw Okoroiwu, J. Etura
Staining of blood films remains one of the most essential part of haematology. The different blood cells have different intracellular structure that take up stains according to chemical nature. Leishman stain is one of the Romanowsky stains used in the haematology laboratory for the staining of blood films. It contains methylene blue and eosin dye prepared in an alcohol medium and is diluted with a buffer. Phosphate buffer is used during Leishman stain as it acts as a mordant enhancing staining reactions and it is the recommended and best buffer for staining. Aim This comparative study was aimed at determining if different water samples used as buffer show significant effects on the morphology of blood cells during Leishman staining. Methods pH analyses of different water sources such as water from borehole, waterboard, rainwater, fridge water, distilled water and air conditioned water were carried out and used as buffers to carry out Leishmann staining on blood films. The stained slides were examined macroscopically and microscopically using 100x objective. Results The study has shown that deionized and tap water used as buffer could produce moderately good film with well stained red cells, white cells and platelets. Rainwater, distilled, fridge and air conditioned water showed a significant change in the morphology of blood cells both in an apparently healthy film and a diseased film Conclusion This study demonstrates that deionized water and tap water devoid of chlorine can be used as substitutes in the right pH of 6.8 in a situation where phosphate buffer is not available. It is therefore recommended that quality control should be carried out to check the quality of stains and buffer used for staining films in order to produce quality results.
{"title":"Comparative analysis of different water samples as Buffer in Leishman stain.","authors":"I. Okafor, G. Anagha, Uchech Henshaw Okoroiwu, J. Etura","doi":"10.59708/ajlhts.v1i2.2219","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i2.2219","url":null,"abstract":"Staining of blood films remains one of the most essential part of haematology. The different blood cells have different intracellular structure that take up stains according to chemical nature. Leishman stain is one of the Romanowsky stains used in the haematology laboratory for the staining of blood films. It contains methylene blue and eosin dye prepared in an alcohol medium and is diluted with a buffer. Phosphate buffer is used during Leishman stain as it acts as a mordant enhancing staining reactions and it is the recommended and best buffer for staining. Aim This comparative study was aimed at determining if different water samples used as buffer show significant effects on the morphology of blood cells during Leishman staining. Methods pH analyses of different water sources such as water from borehole, waterboard, rainwater, fridge water, distilled water and air conditioned water were carried out and used as buffers to carry out Leishmann staining on blood films. The stained slides were examined macroscopically and microscopically using 100x objective. Results The study has shown that deionized and tap water used as buffer could produce moderately good film with well stained red cells, white cells and platelets. Rainwater, distilled, fridge and air conditioned water showed a significant change in the morphology of blood cells both in an apparently healthy film and a diseased film Conclusion This study demonstrates that deionized water and tap water devoid of chlorine can be used as substitutes in the right pH of 6.8 in a situation where phosphate buffer is not available. It is therefore recommended that quality control should be carried out to check the quality of stains and buffer used for staining films in order to produce quality results.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126425919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.59708/ajlhts.v1i2.2216
Tawakalitu O Zakariyahu, I. Ajayi
Haematological features and clinical severity of Sickle Cell Anaemia (SCA) are influenced by age, gender, genetic and community factors. BCL11A is cytogenetically located on short arm of chromosome 2 at position 16.1 while it is located at base pair 60,451,167 to 60,553,498 on chromosome 2 at the molecular level. Polymorphisms in the HBS1L-MYB Intergenic region were associated with F-cell levels and accounted for 19.4% of the F-cell variance in normal Europeans. This study highlights possible effects of age in Haemoglobin F Induction through possible association of BCL11A and HBS1L-MYB genes polymorphism in SCA Subjects population in Oshogbo metropolis. Thirty – Six SCA subjects were recruited with ages ranging between 1 and 40years divided into five groups of eight subjects each except age ranges 21 to 25 and 25 to 40 years which were six subjects each, while ten haemoglobin A subjects served as control for all age ranges. Red Cell indices and gene analysis such as HCT, RBC Count, Haemoglobin concentration, Hb F level, BCL11A and HBS1L-MYB genes were studied using standard methods. Results shows no statistically significant difference in fetal haemoglobin between control and subjects (P>0.05). There was a statistical decrease in haemoglobin level in the test subjects compared to controls. Also, the HBS1L-MYB protein expression was not significantly different in Haemoglobin A subjects and the SCA subjects, but there was a statistically significant decrease in BCL11A gene expression in SCA Subjects compared to Haemoglobin A Subjects (P<0.05). A statistically significant decrease in BCL11A gene expression was obtained in ages 16 to 20 and 21 to 40years age groups (p<0.05). There was a positive correlation between Fetal Haemoglobin and haematocrit and haemoglobin levels (r=0.04341, p=0.2227); (r=0.01705, p=0.4479), respectively. The correlation between Fetal haemoglobin and BCL11A and HBS1L-MYB showed statistically significant negative correlations (r= - 0.1220, p=0.0368), r= - 0.1260, p=0.0336 respectively). Conclusively, BCL11A and HBS1L-MYB genes promote induction of fetal haemoglobin in SCA subjects compared with controls recruited for this study.
{"title":"Association of BCL11A and HBS1L-MYB Polymorphisms in Sickle Cell Anaemia Subjects of Different Age Groups in Nigeria","authors":"Tawakalitu O Zakariyahu, I. Ajayi","doi":"10.59708/ajlhts.v1i2.2216","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i2.2216","url":null,"abstract":"Haematological features and clinical severity of Sickle Cell Anaemia (SCA) are influenced by age, gender, genetic and community factors. BCL11A is cytogenetically located on short arm of chromosome 2 at position 16.1 while it is located at base pair 60,451,167 to 60,553,498 on chromosome 2 at the molecular level. Polymorphisms in the HBS1L-MYB Intergenic region were associated with F-cell levels and accounted for 19.4% of the F-cell variance in normal Europeans. This study highlights possible effects of age in Haemoglobin F Induction through possible association of BCL11A and HBS1L-MYB genes polymorphism in SCA Subjects population in Oshogbo metropolis. Thirty – Six SCA subjects were recruited with ages ranging between 1 and 40years divided into five groups of eight subjects each except age ranges 21 to 25 and 25 to 40 years which were six subjects each, while ten haemoglobin A subjects served as control for all age ranges. Red Cell indices and gene analysis such as HCT, RBC Count, Haemoglobin concentration, Hb F level, BCL11A and HBS1L-MYB genes were studied using standard methods. Results shows no statistically significant difference in fetal haemoglobin between control and subjects (P>0.05). There was a statistical decrease in haemoglobin level in the test subjects compared to controls. Also, the HBS1L-MYB protein expression was not significantly different in Haemoglobin A subjects and the SCA subjects, but there was a statistically significant decrease in BCL11A gene expression in SCA Subjects compared to Haemoglobin A Subjects (P<0.05). A statistically significant decrease in BCL11A gene expression was obtained in ages 16 to 20 and 21 to 40years age groups (p<0.05). There was a positive correlation between Fetal Haemoglobin and haematocrit and haemoglobin levels (r=0.04341, p=0.2227); (r=0.01705, p=0.4479), respectively. The correlation between Fetal haemoglobin and BCL11A and HBS1L-MYB showed statistically significant negative correlations (r= - 0.1220, p=0.0368), r= - 0.1260, p=0.0336 respectively). Conclusively, BCL11A and HBS1L-MYB genes promote induction of fetal haemoglobin in SCA subjects compared with controls recruited for this study.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134071629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.59708/ajlhts.v1i2.2218
U. Obeta, M. Muhibi
Blood banking services has existed in Nigeria for ages for the purposes of transfusion services. Though, there are successes achieved with the blood transfusion services, one of the major challenges is that the whole blood or blood components used cannot serve as sources of stem cells, whereas cord blood can. The unavailability of cord blood banks to provide such services creates a huge gap in the medical services thereby creating opportunities for medical tourism and capital flight.
{"title":"Cord Blood Banking Services in Nigeria; the Earlier the Better","authors":"U. Obeta, M. Muhibi","doi":"10.59708/ajlhts.v1i2.2218","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i2.2218","url":null,"abstract":"Blood banking services has existed in Nigeria for ages for the purposes of transfusion services. Though, there are successes achieved with the blood transfusion services, one of the major challenges is that the whole blood or blood components used cannot serve as sources of stem cells, whereas cord blood can. The unavailability of cord blood banks to provide such services creates a huge gap in the medical services thereby creating opportunities for medical tourism and capital flight.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"246 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124917264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.59708/ajlhts.v1i2.2207
A.I Aruomaren ., jJ.O Osakue, D.O Ikponmuem, O. G. Igharo
Introduction: The toxic effect of Datura metel have been well documented in humans however litter is known on its effect on iron profile status. The objective of this study was to evaluate the effect Datura metel on some iron profile parameters using Drosophila melanogaster model. Materials and Methods: Drosophila melanogaster flies were allocated into four classes (ethanolic leaf extract, ethanolic seed extract, aqueous seed extract and aqueous leaf extract) with six (6) different concentrations. Each concentration were performed in triplicates with twenty-five (25) flies each. Aqueous and ethanolic extract of Datura metel leaf and seed were extracted and different concentrations was administered to Drosophila melanogaster. A survival assay was done for 14 days while Serum iron and Serum ferritin assay was done after 7 days on the various treatment group. Iron and ferritin were estimated using enzyme linked immunosorbent assay (ELISA), ANOVA was used to compare between the different concentrations of the extract and the control group and result reported in mean±SEM. Results: The results obtained showed a significant decrease (p<0.001) in iron concentration in the experimental group treated with different concentrations of aqueous leaf extract of Datura metel when compared with the control. Similarly, there was a significant decrease in iron (P = 0.001) and ferritin (P < 0.001) in the experimental group treated with different concentration of ethanolic extract of Datura metel seed when compared to the controls. The median survival rate shows that after 14 exposure to different concentrations of datura metel, aqueous and ethanolic leaves extracts had a median survival rate of 8 flies while aqueous and ethanolic seed extract had a median survival rate of 11 and 12 flies respectively. Conclusion: The results suggest that ethanolic and aqueous extract of Datura metel seed and leaf decreases iron and ferritin levels in Drosophila melanogaster. And the leaves of Datura metel maybe more toxic than the seed of the plant.
{"title":"Evaluation of Iron, Ferritin levels and the Toxic effect of Datura metel in Drosophila melanogaster model.","authors":"A.I Aruomaren ., jJ.O Osakue, D.O Ikponmuem, O. G. Igharo","doi":"10.59708/ajlhts.v1i2.2207","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i2.2207","url":null,"abstract":"Introduction: The toxic effect of Datura metel have been well documented in humans however litter is known on its effect on iron profile status. The objective of this study was to evaluate the effect Datura metel on some iron profile parameters using Drosophila melanogaster model. Materials and Methods: Drosophila melanogaster flies were allocated into four classes (ethanolic leaf extract, ethanolic seed extract, aqueous seed extract and aqueous leaf extract) with six (6) different concentrations. Each concentration were performed in triplicates with twenty-five (25) flies each. Aqueous and ethanolic extract of Datura metel leaf and seed were extracted and different concentrations was administered to Drosophila melanogaster. A survival assay was done for 14 days while Serum iron and Serum ferritin assay was done after 7 days on the various treatment group. Iron and ferritin were estimated using enzyme linked immunosorbent assay (ELISA), ANOVA was used to compare between the different concentrations of the extract and the control group and result reported in mean±SEM. Results: The results obtained showed a significant decrease (p<0.001) in iron concentration in the experimental group treated with different concentrations of aqueous leaf extract of Datura metel when compared with the control. Similarly, there was a significant decrease in iron (P = 0.001) and ferritin (P < 0.001) in the experimental group treated with different concentration of ethanolic extract of Datura metel seed when compared to the controls. The median survival rate shows that after 14 exposure to different concentrations of datura metel, aqueous and ethanolic leaves extracts had a median survival rate of 8 flies while aqueous and ethanolic seed extract had a median survival rate of 11 and 12 flies respectively. Conclusion: The results suggest that ethanolic and aqueous extract of Datura metel seed and leaf decreases iron and ferritin levels in Drosophila melanogaster. And the leaves of Datura metel maybe more toxic than the seed of the plant.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130568120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.59708/ajlhts.v1i2.2217
D. Abunimye, E. Akwiwu, S. Anyanwu, Eme. E Onukak, J. Akpotuzor
Introduction: Although viremia has been greatly addressed in the management of human immunodeficiency virus (HIV) infection by the advancement in antiretroviral therapy, not all HIV-associated morbidities have been resolved. Observations of increased cardiovascular risk in relation to antiretroviral therapy have been reported. Full blood count continues to be useful in disease management, and efforts are directed towards optimising its utility in medical practice. Derivatives of blood cell counts have in recent times proved to be informative with regards to the inflammatory-thrombotic cycle. The utility of these derived parameters in HIV within the study locality is worth exploring. Methods: This single-site study was carried out at University of Calabar Teaching Hospital in Calabar, Cross River State of Nigeria. White blood cell and platelet counts were carried out by automation, while blood cell ratios were calculated. Statistical analysis of data was done using SPSS 22.0. A p-value of ≤ 0.05 was considered to infer a statistically significant difference. Results: Significant reductions of white blood cell parameters were recorded in HIV, particularly among infected persons on antiretroviral therapy. Platelet count and plateletcrit were significantly lower, while mean platelet volume and platelet distribution width were higher in newly diagnosed persons compared to HIV-infected subjects on therapy and control subjects. Platelet-to-lymphocyte ratio was significantly higher among subjects on therapy compared to the rest of the groups. Conclusion: Increase in platelet count following antiretroviral therapy could be posing a risk of platelet-driven morbidities as typified in the observed elevated thrombotic marker.
{"title":"SELECTED INFLAMMATORY AND THROMBOTIC INDICATORS AMONG PERSONS LIVING WITH HIV INFECTION IN SOUTHERN NIGERIA","authors":"D. Abunimye, E. Akwiwu, S. Anyanwu, Eme. E Onukak, J. Akpotuzor","doi":"10.59708/ajlhts.v1i2.2217","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i2.2217","url":null,"abstract":"Introduction: Although viremia has been greatly addressed in the management of human immunodeficiency virus (HIV) infection by the advancement in antiretroviral therapy, not all HIV-associated morbidities have been resolved. Observations of increased cardiovascular risk in relation to antiretroviral therapy have been reported. Full blood count continues to be useful in disease management, and efforts are directed towards optimising its utility in medical practice. Derivatives of blood cell counts have in recent times proved to be informative with regards to the inflammatory-thrombotic cycle. The utility of these derived parameters in HIV within the study locality is worth exploring. Methods: This single-site study was carried out at University of Calabar Teaching Hospital in Calabar, Cross River State of Nigeria. White blood cell and platelet counts were carried out by automation, while blood cell ratios were calculated. Statistical analysis of data was done using SPSS 22.0. A p-value of ≤ 0.05 was considered to infer a statistically significant difference. Results: Significant reductions of white blood cell parameters were recorded in HIV, particularly among infected persons on antiretroviral therapy. Platelet count and plateletcrit were significantly lower, while mean platelet volume and platelet distribution width were higher in newly diagnosed persons compared to HIV-infected subjects on therapy and control subjects. Platelet-to-lymphocyte ratio was significantly higher among subjects on therapy compared to the rest of the groups. Conclusion: Increase in platelet count following antiretroviral therapy could be posing a risk of platelet-driven morbidities as typified in the observed elevated thrombotic marker.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127361037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.59708/ajlhts.v1i2.2214
N. Garba, S. Ahmad, U.A Makuku, I. Ajayi
Background: Acute myeloid leukaemia (AML) is a heterogeneous clonal disorder characterized by abnormal proliferation of immature and non-functional cells known as blasts and subsequently results in bone marrow failure and organs infiltrations. Blood group antigens have been linked with the aetiology and pathophysiology of various communicable and non-communicable diseases. Objective: This study therefore investigated the association of some blood group phenotypes with AML among subjects in Kano, Nigeria. Materials and Methods: Acute myeloid leukaemia was diagnosed using the clinical and cytological criteria in Aminu Kano Teaching Hospital (AKTH). The blood groups were determined using tube method for ABO, Rh-D, Lewis, Duffy and MNS while Rh-C-c-E-e and Kell by Gel method. The Antisera and Gel cards were obtained from Lorne Laboratories in the United Kingdom. All the techniques were according to the manufacturer’s instructions. Data was presented as tables and analysed using SPSS (version 25.0). Results: A total of 25 AML subjects (age: 18.9±13.9 years, male/female ratio: 1.3:1) were studied with 25 healthy blood donors (controls) with mean ages of 29.2±5.3 years and male/female ratio: 11.5:1. Association of some blood groups and AML was determined using Chi-square test while Odds Ratio (OR) and Relative Risk (RR) were used to ascertain their risk values. The results showed that Lea, Leb, K and M antigens were significantly associated with AML (OR: 4.75, 7.11, 11.29 and 7.67 respectively). Conclusion: We conclude that individuals with Lewis antigens (Lea and Leb), Kell antigen (K) and M antigen may have increased risk of developing acute myeloid leukaemia.
{"title":"ASSOCIATION OF SOME BLOOD GROUP PHENOTYPES AND RISK OF ACUTE MYELOID LEUKAEMIA IN KANO, NIGERIA","authors":"N. Garba, S. Ahmad, U.A Makuku, I. Ajayi","doi":"10.59708/ajlhts.v1i2.2214","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i2.2214","url":null,"abstract":"Background: Acute myeloid leukaemia (AML) is a heterogeneous clonal disorder characterized by abnormal proliferation of immature and non-functional cells known as blasts and subsequently results in bone marrow failure and organs infiltrations. Blood group antigens have been linked with the aetiology and pathophysiology of various communicable and non-communicable diseases. Objective: This study therefore investigated the association of some blood group phenotypes with AML among subjects in Kano, Nigeria. Materials and Methods: Acute myeloid leukaemia was diagnosed using the clinical and cytological criteria in Aminu Kano Teaching Hospital (AKTH). The blood groups were determined using tube method for ABO, Rh-D, Lewis, Duffy and MNS while Rh-C-c-E-e and Kell by Gel method. The Antisera and Gel cards were obtained from Lorne Laboratories in the United Kingdom. All the techniques were according to the manufacturer’s instructions. Data was presented as tables and analysed using SPSS (version 25.0). Results: A total of 25 AML subjects (age: 18.9±13.9 years, male/female ratio: 1.3:1) were studied with 25 healthy blood donors (controls) with mean ages of 29.2±5.3 years and male/female ratio: 11.5:1. Association of some blood groups and AML was determined using Chi-square test while Odds Ratio (OR) and Relative Risk (RR) were used to ascertain their risk values. The results showed that Lea, Leb, K and M antigens were significantly associated with AML (OR: 4.75, 7.11, 11.29 and 7.67 respectively). Conclusion: We conclude that individuals with Lewis antigens (Lea and Leb), Kell antigen (K) and M antigen may have increased risk of developing acute myeloid leukaemia.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122249924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-30DOI: 10.59708/ajlhts.v1i1.2209
Ransom Baribefii Jacob, T. Warmate, B. Moore-Igwe, Zaccheaus Awortu. Jeremiah
Introduction: The presence of rare blood group antibodies in the blood of donors/recipients stimulate red cell alloimmunization and constitute a major cause of transfusion related reaction after a compatible ABO cross match, yet remain one of the less routinely assessed prior to blood donation and transfusion in Nigeria. This cross-sectional study was carried out among indigenes of Bonny kingdom in Rivers State Nigeria to access the percentage expression of Lewis B, Rh-e, N and ABO antigens in descent of Bonny Kingdom Rivers State Nigeria. Materials and Methods:- One hundred and twenty (120) apparently healthy subjects consisting of sixty (60) females and sixty (60) males aged between 18-50 years all indigenes of Bonny Kingdom were recruited by structured questionnaire for the study. 4mls of venipuncture blood was aseptically collected from each participant into vacutainer bottle containing 0.5 mL of 1.2 mg/mL dipottasium ethylenediaminetetraacetic acid anticoagulant and a 5% cell suspension was prepared and used to determine the various blood groups antigens using the standard tube Agglutination technique. Results: Results obtained shows a total of 94.1% (113) of the participants expressed Rh-e antigen, 45.8% (55) male and 48.3% (58) females. 7.5% (9) of the participants expressed the N antigen, 3.3% (4) male and 4.2% (5) females. 4.2% (5) of the study population expressed Lewis B antigen, 2.5% (3) males and 1.7% (2) female. The ABO system showed 68.5% (79) O blood group, 29.1% (35) male and 36.7% (44) female. 22(18.3%) typed for A blood group, 11.6% (14) male and 6.7% (8) female. For B blood group 14.2% (17) persons were typed, 7.5% (9) male and 6.7% (8) female while 1.6% (2) persons were typed as AB and they are all females. Conclusion: Rh-e phenotype occurred highest among the study participants. The proportion of the population without the expression of these antigens of the blood group tested has the potential to be alloimmunized during blood transfusion and develop antibodies, some of which can be responsible for transfusion reactions and haemolytic disease of the new born. This study has provided baseline data on the distribution of some blood group antigens in the Bonny Kingdom of Rivers State in Nigeria. Based on the finding in this study, it is recommended that Rh-e grouping be carried out on pregnant mothers, blood donors and recipients before transfusion; while Lewis B and N blood groups may be subjected to expansive population testing.
在献血者/受者的血液中存在罕见血型抗体会刺激红细胞异体免疫，并构成相容ABO交叉配型后输血相关反应的主要原因，但在尼日利亚，这仍然是献血和输血前较少常规评估的一项。本横断面研究在尼日利亚河流州邦尼王国的土著人中进行，以获得刘易斯B, Rh-e, N和ABO抗原在尼日利亚河流州邦尼王国后裔中的表达百分比。材料与方法:采用结构化问卷调查的方法，招募表面健康的120名受试者，其中女性60名，男性60名，年龄在18-50岁之间，均为邦尼王国原住民。每位受试者无菌采集静脉穿刺血4ml，装进含有0.5 mL 1.2 mg/mL二钾乙二胺四乙酸抗凝剂的真空瓶中，制备5%细胞悬液，采用标准试管凝集法测定各种血型抗原。结果:共有94.1%(113例)的参与者表达Rh-e抗原，其中男性55例(45.8%)，女性58例(48.3%)。7.5%(9人)的参与者表达N抗原，其中男性3.3%(4人)，女性4.2%(5人)。4.2%(5)的研究人群表达Lewis B抗原，其中男性2.5%(3)，女性1.7%(2)。O型血占68.5%(79)，男性占29.1%(35)，女性占36.7%(44)。A型22例(18.3%)，男性14例(11.6%)，女性8例(6.7%)。B型14.2%(17人)，男性7.5%(9人)，女性6.7%(8人)，AB型1.6%(2人)，均为女性。结论:Rh-e表型在研究参与者中发生率最高。在被检测的血型中，没有这些抗原表达的人群的比例有可能在输血过程中产生同种免疫并产生抗体，其中一些抗体可能导致输血反应和新生儿的溶血性疾病。这项研究提供了尼日利亚河州邦尼王国某些血型抗原分布的基线数据。根据本研究结果，建议在输血前对孕妇、献血者和受血者进行Rh-e分组;而刘易斯B和N血型的人可能需要进行广泛的人群测试。
{"title":"Percentage Expression of Lewis B, Rh-e, N and ABO Antigens in descents of Bonny Kingdom, River, State, Nigeria.","authors":"Ransom Baribefii Jacob, T. Warmate, B. Moore-Igwe, Zaccheaus Awortu. Jeremiah","doi":"10.59708/ajlhts.v1i1.2209","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i1.2209","url":null,"abstract":"Introduction: The presence of rare blood group antibodies in the blood of donors/recipients stimulate red cell alloimmunization and constitute a major cause of transfusion related reaction after a compatible ABO cross match, yet remain one of the less routinely assessed prior to blood donation and transfusion in Nigeria. This cross-sectional study was carried out among indigenes of Bonny kingdom in Rivers State Nigeria to access the percentage expression of Lewis B, Rh-e, N and ABO antigens in descent of Bonny Kingdom Rivers State Nigeria. Materials and Methods:- One hundred and twenty (120) apparently healthy subjects consisting of sixty (60) females and sixty (60) males aged between 18-50 years all indigenes of Bonny Kingdom were recruited by structured questionnaire for the study. 4mls of venipuncture blood was aseptically collected from each participant into vacutainer bottle containing 0.5 mL of 1.2 mg/mL dipottasium ethylenediaminetetraacetic acid anticoagulant and a 5% cell suspension was prepared and used to determine the various blood groups antigens using the standard tube Agglutination technique. Results: Results obtained shows a total of 94.1% (113) of the participants expressed Rh-e antigen, 45.8% (55) male and 48.3% (58) females. 7.5% (9) of the participants expressed the N antigen, 3.3% (4) male and 4.2% (5) females. 4.2% (5) of the study population expressed Lewis B antigen, 2.5% (3) males and 1.7% (2) female. The ABO system showed 68.5% (79) O blood group, 29.1% (35) male and 36.7% (44) female. 22(18.3%) typed for A blood group, 11.6% (14) male and 6.7% (8) female. For B blood group 14.2% (17) persons were typed, 7.5% (9) male and 6.7% (8) female while 1.6% (2) persons were typed as AB and they are all females. Conclusion: Rh-e phenotype occurred highest among the study participants. The proportion of the population without the expression of these antigens of the blood group tested has the potential to be alloimmunized during blood transfusion and develop antibodies, some of which can be responsible for transfusion reactions and haemolytic disease of the new born. This study has provided baseline data on the distribution of some blood group antigens in the Bonny Kingdom of Rivers State in Nigeria. Based on the finding in this study, it is recommended that Rh-e grouping be carried out on pregnant mothers, blood donors and recipients before transfusion; while Lewis B and N blood groups may be subjected to expansive population testing.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123683793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-30DOI: 10.59708/ajlhts.v1i1.2206
S. Christian, I. Elekima, B. Moore-Igwe, Iloloba Mac-Aworu, Bernice. C Ariolu
Introduction: There is a disturbing trend with intake of un-prescribed doses of multivitamin syrup as a recovering medication after illness since it is believed to boost blood blood cell production. This study aims to assess the effect of De-Deon haemoglobin syrup on Vitamin B12, Folate Levels and some Haematological Parameters among Females in Port Harcourt, Nigeria. Methods: Ten (10) apparently healthy female subjects between the ages of 20 to 30 years were recruited for the study; blood samples were collected via standard vein-puncture technique before administration of the drug; on the fourth (4th) day, after day 3 of drug administration; and after day 6 of drug administration. Vitamin B12, and folate were analyzed using ELISA method; while haematological parameters were determined using Sysmex autoanalyzer. Data were analyzed using Statistical Package for Social Sciences computer database (Version 10.0; SPSS Inc., Chicago, IL, USA) for mean, standard deviation, analysis of variance and correlation, p-value of <0.05 was considered statistically significant. Results: showed no significant change (p>0.05) in vitamin B12, folate, red blood cells (RBC), white blood cells (WBC), haemoglobin (Hb), haematocrit (HCT) and platelet count before treatment (Day 1), during treatment (day 3) and after treatment (day 6) of De-Deon syrup administration. Correlation analysis of Vitamin B12 against haematological parameters for day, 1, 3, and after day 6 showed significant negative correlation against haemoglobin level (r = 0.6731; p = 0.0373) and statistically significant positive correlation against platelets (r = 0.6731; p = 0.0165) after day 1. Statistically significant positive correlation of vitamin B12 against platelets (r = 0.6795; p = 0.0153) was observed after day 3; no significant statistically significant correlation in levels of vitamin B12 against other haematological parameters were observed. No significant correlation was also observed for folate and haematological parameters after day 1, 3, and 6. Conclusion: The study has therefore revealed that De-Deon syrup of haemoglobin when taken within a short period of time (acute administration), showed no statistically significant effect on vitamin B12, folate and haematological parameters. However, vitamin B12 was increased albeit without any significance. Although no significance was observed in the study, the significant positive correlation between vitamin B12 and platelet count points out the relevance of vitamin B12 supplementation as it is required in the metabolism of every cell of the human body and also a cofactor in DNA synthesis in both fatty acid and amino acid metabolism.
简介:有一种令人不安的趋势，即服用非处方剂量的复合维生素糖浆作为疾病后的恢复药物，因为它被认为可以促进血液细胞的产生。本研究旨在评估De-Deon血红蛋白糖浆对尼日利亚哈科特港女性维生素B12、叶酸水平和一些血液学参数的影响。方法:招募年龄在20 ~ 30岁、身体明显健康的女性受试者10名;给药前采用标准静脉穿刺法采血;给药第3天后第4天;在给药的第六天之后。ELISA法分析维生素B12、叶酸;采用Sysmex自动分析仪测定血液学参数。数据分析使用Statistical Package for Social Sciences计算机数据库(Version 10.0;SPSS Inc.， Chicago, IL, USA)对De-Deon糖浆治疗前(第1天)、治疗期间(第3天)和治疗后(第6天)维生素B12、叶酸、红细胞(RBC)、白细胞(WBC)、血红蛋白(Hb)、红细胞压积(HCT)和血小板计数的平均值、标准差、方差和相关性分析，p值为0.05。维生素B12与第一天、第1天、第3天和第6天后的血液学参数的相关分析显示，维生素B12与血红蛋白水平呈显著负相关(r = 0.6731;P = 0.0373)，与血小板呈显著正相关(r = 0.6731;P = 0.0165)。维生素B12与血小板呈显著正相关(r = 0.6795;P = 0.0153);维生素B12水平与其他血液学参数之间没有统计学上的显著相关性。在第1、3和6天，叶酸和血液学参数也没有明显的相关性。结论:本研究表明，在短时间内(急性给药)服用De-Deon血红蛋白糖浆，对维生素B12、叶酸和血液学参数没有统计学意义的影响。然而，维生素B12增加了，尽管没有任何意义。虽然本研究未发现显著性，但维生素B12与血小板计数之间的显著正相关表明补充维生素B12的相关性，因为它是人体每个细胞代谢所必需的，也是脂肪酸和氨基酸代谢中DNA合成的辅助因子。
{"title":"Assessment of Vitamin B12, Folate Levels and some Haematological Parameters among Females on De-Deon syrup in Port Harcourt, Nigeria","authors":"S. Christian, I. Elekima, B. Moore-Igwe, Iloloba Mac-Aworu, Bernice. C Ariolu","doi":"10.59708/ajlhts.v1i1.2206","DOIUrl":"https://doi.org/10.59708/ajlhts.v1i1.2206","url":null,"abstract":"Introduction: There is a disturbing trend with intake of un-prescribed doses of multivitamin syrup as a recovering medication after illness since it is believed to boost blood blood cell production. This study aims to assess the effect of De-Deon haemoglobin syrup on Vitamin B12, Folate Levels and some Haematological Parameters among Females in Port Harcourt, Nigeria. Methods: Ten (10) apparently healthy female subjects between the ages of 20 to 30 years were recruited for the study; blood samples were collected via standard vein-puncture technique before administration of the drug; on the fourth (4th) day, after day 3 of drug administration; and after day 6 of drug administration. Vitamin B12, and folate were analyzed using ELISA method; while haematological parameters were determined using Sysmex autoanalyzer. Data were analyzed using Statistical Package for Social Sciences computer database (Version 10.0; SPSS Inc., Chicago, IL, USA) for mean, standard deviation, analysis of variance and correlation, p-value of <0.05 was considered statistically significant. Results: showed no significant change (p>0.05) in vitamin B12, folate, red blood cells (RBC), white blood cells (WBC), haemoglobin (Hb), haematocrit (HCT) and platelet count before treatment (Day 1), during treatment (day 3) and after treatment (day 6) of De-Deon syrup administration. Correlation analysis of Vitamin B12 against haematological parameters for day, 1, 3, and after day 6 showed significant negative correlation against haemoglobin level (r = 0.6731; p = 0.0373) and statistically significant positive correlation against platelets (r = 0.6731; p = 0.0165) after day 1. Statistically significant positive correlation of vitamin B12 against platelets (r = 0.6795; p = 0.0153) was observed after day 3; no significant statistically significant correlation in levels of vitamin B12 against other haematological parameters were observed. No significant correlation was also observed for folate and haematological parameters after day 1, 3, and 6. Conclusion: The study has therefore revealed that De-Deon syrup of haemoglobin when taken within a short period of time (acute administration), showed no statistically significant effect on vitamin B12, folate and haematological parameters. However, vitamin B12 was increased albeit without any significance. Although no significance was observed in the study, the significant positive correlation between vitamin B12 and platelet count points out the relevance of vitamin B12 supplementation as it is required in the metabolism of every cell of the human body and also a cofactor in DNA synthesis in both fatty acid and amino acid metabolism.","PeriodicalId":380612,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116894760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：