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Hygienic and sanitary characteristics of dry salted fish marketed in Porto Velho city, Rondônia – Brazil 在韦柳港市销售的干咸鱼的卫生和卫生特性,Rondônia -巴西
Q4 Veterinary Pub Date : 2022-08-16 DOI: 10.21708/avb.2022.16.3.10747
R. B. Pontuschka, Analice Timoteo de Araujo, Mayline Álex Contes da Silva, J. V. Dantas Filho, R. P. Marmentini, S. V. Schons, J. Cavali
Handcrafted salted fish is marketed weekly in retail markets and public fairs in the Porto Velho city, Rondônia sate, Brazil. Knowing the microbiological quality of these products is essential for public health, given that such products are not subject to any quality control. The aimed of the study was to evaluate the hygienic-sanitary characteristics and the moisture content of pirarucu and salted and dried shrimp marketed in the Porto Velho city. Twenty samples were collected, 10 pirarucu and 10 shrimp, in February and June 2019. For microbiological analysis, surface plating was performed using acidified potato agar, for molds and yeasts, and PCA agar for halophilic bacterias. Moisture was determined by gravimetry, using an oven at 105° C. Results for molds and yeasts on pirarucu ranged <10 log CFU/g (absent) to 3 log CFU/g. For shrimp, values ranged <10 log CFU/g to 3.57 log CFU/g. For halophilic bacterias, contents ranged <10 log CFU/g to 6.30 log CFU/g in pirarucu samples and <10 log CFU/g to 6.97 log CFU/g in shrimp samples. The variation in moisture content ranged 36.99 to 54.31% for pirarucu, and 40.6 to 56.82% for shrimp. The results obtained may be related to poor hygienic conditions in processing, the lack of hygiene of utensils and places for handling, or even the quality of the raw material used.
手工咸鱼每周在巴西的韦柳港市(Rondônia sate)的零售市场和公共集市上销售。鉴于这些产品不受任何质量控制,了解这些产品的微生物质量对公共卫生至关重要。本研究的目的是评价在韦柳港市销售的皮拉鲁库虾、盐虾和干虾的卫生特性和水分含量。2019年2月和6月采集了20份样本,其中10份是皮拉鲁鱼,10份是对虾。为了进行微生物学分析,使用酸化马铃薯琼脂进行表面电镀,用于霉菌和酵母,PCA琼脂用于嗜盐细菌。在105°c的烤箱中,通过重量法测定水分。结果表明,皮拉鲁库上的霉菌和酵母菌的含量范围为<10 log CFU/g(不存在)至3 log CFU/g。对虾的值范围为<10 log CFU/g ~ 3.57 log CFU/g。对于嗜盐细菌,皮罗鱼样品中含量<10 ~ 6.30 log CFU/g,对虾样品中含量<10 ~ 6.97 log CFU/g。水分含量变化幅度为36.99 ~ 54.31%,对虾40.6 ~ 56.82%。所获得的结果可能与加工过程中的卫生条件差,处理工具和场所的卫生不足,甚至与所用原料的质量有关。
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引用次数: 1
Proximal composition, fatty acid profile, omegas and lipid quality in the tambaqui (Colossoma macropomum Cuvier, 1818) (Serrasalmidae) in “flatted cut” “扁平切口”中坦巴基(Colossoma macropomum Cuvier,1818)(Serrasalmidae)的近端成分、脂肪酸图谱、omegas和脂质质量
Q4 Veterinary Pub Date : 2022-08-16 DOI: 10.21708/avb.2022.16.3.10821
J. Cavali, Reginaldo da Silva Francisco, J. V. Dantas Filho, R. B. Pontuschka, Tiago Lucena da Silva, Rodrigo Vieira Alves Amaral
The aimed of this study was to determine proximal composition, fatty acid profile, omegas and lipid quality in the tambaqui (Colossoma macropomum) in “flatted cut”, produced and commercializeded by Rondônia state, Brazil. Three pieces of flatted tambaqui were collected from 40 fish weighing 1.10 ± 0.10 kg. Proximal composition were determined by AOAC Official method 969.23 and 968.08. The fatty acids were grouped to calculate ratio ∑PUFAs/∑SFAs and proportion ∑PUFAs (n-6/n-3), atherogenicity index (AI) were calculated, thrombogenicity index (TI), and ratio between hypocholesterolemic and hypercholesterolemic fatty acids (HH). Were found 1.06 g/100g of mineral matter, 17.64 g/100g of crude protein, 5.74 g/100g of lipids, 75 .54 g/100g of moisture and 122.22 kcal/100g of caloric value. Lipid composition 39.425% of SFAs, 43.518% of MUFAs and 17.057% of PUFAs. It also presented ∑PUFAs/∑SFAs ratios of 2.31 and ∑PUFAs (n-6/n-3) of 3.78. As AI of 0.44, TI of 0.92 and HH of 2.02. Tambaqui meat of 1.10 ± 0.10 kg in flatted cut contains good proximal contents, with high protein content and excellent lipid content, being considered a low-fat meat, with low moisture content and low caloric value and rich in minerals. It can also be considered a good source of MUFAs and PUFAs, especially n-3 and n-6, with high nutritional value, in addition to essential fatty acids, ALA, AA, DHA and EPA, which were crucial to provide good levels of lipid quality.
本研究的目的是确定巴西Rondônia国家生产和商业化的“扁平切割”的tambaqui (Colossoma macropomum)的近端组成、脂肪酸特征、omega和脂质质量。从体重1.10±0.10 kg的40条鱼中采集了3片扁平的tambaqui。采用AOAC官方方法969.23和968.08测定近端成分。将脂肪酸分组计算∑PUFAs/∑SFAs比值和∑PUFAs比值(n-6/n-3),计算动脉粥样硬化指数(AI)、血栓形成指数(TI)和低胆固醇血症与高胆固醇血症比值(HH)。矿物质含量为1.06 g/100g,粗蛋白质含量为17.64 g/100g,脂质含量为5.74 g/100g,水分含量为75.54 g/100g,热值为122.22 kcal/100g。脂质组成:sfa占39.425%,mufa占43.518%,pufa占17.057%。∑PUFAs/∑SFAs比值为2.31,∑PUFAs (n-6/n-3)为3.78。AI为0.44,TI为0.92,HH为2.02。扁切后1.10±0.10 kg的坦巴基肉近端含量好,蛋白质含量高,脂肪含量好,是一种低脂肉,水分含量低,热值低,矿物质丰富。它也可以被认为是MUFAs和PUFAs的良好来源,特别是n-3和n-6,具有很高的营养价值,此外还有必需脂肪酸,ALA, AA, DHA和EPA,这些脂肪酸对提供良好的脂质质量至关重要。
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引用次数: 1
Detection of Salmonella spp. in eggs for human consumption from poultry farms in municipalities of Alagoas State, Brazil 巴西阿拉戈斯州市家禽养殖场供人类食用的鸡蛋中沙门氏菌的检测
Q4 Veterinary Pub Date : 2022-08-16 DOI: 10.21708/avb.2022.16.3.10440
Artur Bibiano de Vasconcelos, Danilo Alves de França, M. N. Ferreira, Dayane Kelly Gomes de Oliveira Araújo, Catarina Bibiano de Vasconcelos, K. Silva
Salmonellosis is a disease that worries poultry farmers and can seriously impact the food safety of the population that consumes products of animal origin. The present study sought to detect the presence of Salmonella spp. in eggs intended for consumption from family poultry farms in municipalities of Alagoas State. The study was carried out from eight farms, where the sample from each farm was obtained by making pools of shell and internal contents of six randomly selected eggs. The pools were submitted to microbiological culture and the colonies were characterized and evaluated by means of laboratory tests. To this end, 50% (4/8) of the shell samples and 75% (6/8) of the internal contents of the eggs were positive for Salmonella spp. In addition, Klebsiella pneumoniae 12.5% (1/8) and Proteus spp. 25% (2/8) were found in the shell samples, and Yersinia spp. 12.5% (1/8) in the internal contents of the eggs. Salmonella spp. and other enterobacteria were confirmed to occur in eggs intended for consumption. The way the birds were raised did not seem to have a significant influence on the results obtained, and the presence of passerines on the farms may have contributed to the existence of bacteria there. Being aware of the risk to public health that some of these bacteria can present, it is necessary to take decisions that support the small producer in search of food safety for all.
沙门氏菌病是一种令家禽养殖户担忧的疾病,会严重影响食用动物源性产品的人群的食品安全。本研究旨在检测阿拉戈斯州各城市家庭家禽养殖场食用的鸡蛋中是否存在沙门氏菌。这项研究在八个农场进行,每个农场的样本是通过制作六个随机选择的鸡蛋的外壳和内部内容物池来获得的。将池进行微生物培养,并通过实验室测试对菌落进行表征和评估。为此,50%(4/8)的外壳样品和75%(6/8)的鸡蛋内部内容物对沙门氏菌呈阳性。此外,肺炎克雷伯菌12.5%(1/8)和变形杆菌25%(2/8)在外壳样品中发现,耶尔森菌12.5%(1/8)在鸡蛋内容物中发现。沙门氏菌和其他肠道细菌被证实存在于供食用的鸡蛋中。饲养鸟类的方式似乎对获得的结果没有重大影响,农场中雀形目的存在可能是细菌存在的原因之一。意识到其中一些细菌可能对公众健康造成的风险,有必要做出支持小生产者为所有人寻求食品安全的决定。
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引用次数: 0
Cytopathology of cutaneous and subcutaneous neoplasms in feline species: a retrospective study 猫科动物皮肤和皮下肿瘤的细胞病理学:一项回顾性研究
Q4 Veterinary Pub Date : 2022-08-16 DOI: 10.21708/avb.2022.16.3.10717
K. Filgueira, M. C. Chalita, F. P. Sellera, Archivaldo Reche-Júnior
It is estimated that a quarter of all feline neoplasms affect the integumentary system. Cytological examination is of admirable importance for detection of neoplasms in companion animals, being considered a simple and non-invasive method. To date, there are limited information regarding the prevalence of feline skin cancer as well as the use of cytopathology as a diagnostic tool. In this sense, the study aimed to characterize cutaneous and subcutaneous tumors in 192 cats from the archives of a private clinic in São Paulo-SP, Brazil, specialized in the care of felines. Data regarding the animal (breed definition, sex, and age group) and tumor characteristics (biological behavior, cytomorphological category of neoplasm, and cytological suggestion for neoplasm) were obtained in a five-year period. A 95% confidence interval (CI) was estimated to verify the statistical difference between the animal and tumor characteristics. The chi-square test was carried out to verify the association between the tumor biological behavior and animal variables, as well as other tumor characteristics. Most cats were mixed breed and aged 11 to 15 years. Malignant tumors were predominant. A statistical association was observed between cytomorphological category of neoplasm and biological behavior (p<0.001). Mesenchymal (24.5%) and epithelial tumors (25%) presented the highest frequency among those benign and malignant, respectively. The cytological suggestion for neoplasm was also statistically associated with biological behavior (p<0.001). The highest percentage of occurrence of benign and malignant tumors consisted of lipomas (22.9%) and sarcomas (19.3%), respectively.
据估计,四分之一的猫科动物肿瘤会影响牙龈系统。细胞学检查对于检测伴侣动物的肿瘤具有令人钦佩的重要性,被认为是一种简单且无创的方法。到目前为止,关于猫皮肤癌症的患病率以及细胞病理学作为诊断工具的使用,信息有限。从这个意义上说,这项研究旨在描述巴西圣保罗一家专门照顾猫科动物的私人诊所档案中192只猫的皮肤和皮下肿瘤。在五年内获得了有关动物(品种定义、性别和年龄组)和肿瘤特征(生物学行为、肿瘤的细胞形态学类别和肿瘤的细胞学建议)的数据。估计95%置信区间(CI)以验证动物和肿瘤特征之间的统计差异。卡方检验用于验证肿瘤生物学行为与动物变量以及其他肿瘤特征之间的相关性。大多数猫都是混血猫,年龄在11到15岁之间。恶性肿瘤占主导地位。肿瘤的细胞形态类型与生物学行为之间存在统计学相关性(p<0.001)。间充质肿瘤(24.5%)和上皮肿瘤(25%)在良性和恶性肿瘤中的发病率最高。肿瘤的细胞学提示也与生物学行为有统计学意义(p<0.001)。良性和恶性肿瘤的发生率最高,分别为脂肪瘤(22.9%)和肉瘤(19.3%)。
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引用次数: 0
Mineral composition, omegas and lipid quality in the visceral fat residues of tambaqui (Colossoma macropomum Cuvier, 1818) 坦巴基内脏脂肪残留物中的矿物质成分、omegas和脂质质量(Colossoma macropomum Cuvier,1818)
Q4 Veterinary Pub Date : 2022-08-16 DOI: 10.21708/avb.2022.16.3.10790
J. Cavali, Reginaldo da Silva Francisco, J. V. Dantas Filho, R. B. Pontuschka, Tiago Lucena da Silva, Rodrigo Vieira Alves Amaral
This study aimed to determine the mineral composition, fatty acid profile, omegas and lipid quality indexes in the visceral fat residues of tambaqui (C. macropomum). Three pieces of visceral fat were collected from 20 fish weighing 1.10 ± 0.10 kg, which were homogenized and sent for compositional analysis. Minerals were determined by AOAC Official method 969.23 and 968.08. The fatty acids were grouped to calculate the ∑PUFAs/∑SFAs fatty acids ratio and the proportion of polyunsaturated fatty acids ∑PUFAs (n-6/n-3), atherogenicity indixes (AI) were calculated, thrombogenicity (TI), and ratio between hypocholesterolemic and hypercholesterolemic fatty acids (HH). Mineral elements were found, 0.68 ± 0.015 mg/100g of total iron, 159.16 ± 14.32 mg/100g of Na+, 63.90 ± 5.11 mg/100g of K+, 10.28 ± 0. 62 mg/100g of Ca2+and 7.31 ± 0.58 mg/100g of Mg2+. As for fatty acids, 40.10% of SFAs, 38.10% of MUFAs and 21.80% of PUFAs. The calculations indicated significant values of omegas, 3, 6, 7 and n-7. There were ∑PUFAs/∑SFAs ratios of 1.84 and ∑PUFAs(n-6/n-3) of 6.22, with an AI of 0.50 and a TI of 0.93 and HH of 2.16. The 1.0 ± 0.10 kg tambaqui visceral fat residues can be evaluated as having high nutritional value, in addition to being a viable option for oil extraction and inclusion in animal feed.
本研究旨在测定坦巴基(C.macropomum)内脏脂肪残留物中的矿物质组成、脂肪酸组成、omegas和脂质质量指标。从20条体重1.10±0.10 kg的鱼身上采集了三块内脏脂肪,将其均质化并送去进行成分分析。矿物通过AOAC官方方法969.23和968.08进行测定。将脂肪酸分组计算∑PUFAs/∑SFAs脂肪酸比例和多不饱和脂肪酸比例∑PUFAs(n-6/n-3),计算动脉粥样硬化指数(AI)、血栓形成性(TI)以及低胆固醇血症和高胆固醇血症脂肪酸之比(HH)。矿物元素含量为:全铁0.68±0.015mg/100g,Na+159.16±14.32mg/100g,K+63.90±5.11mg/100g,10.28±0。Ca2+浓度为62 mg/100g,Mg2+浓度为7.31±0.58 mg/100g。就脂肪酸而言,40.10%的SFAs、38.10%的MUFA和21.80%的PUFA。计算表明,omegas,3,6,7和n-7的数值非常显著。∑PUFAs/∑SFAs比值为1.84,∑PUFAs(n-6/n-3)为6.22,AI为0.50,TI为0.93,HH为2.16。1.0±0.10 kg的坦巴基内脏脂肪残留物可以被评估为具有高营养价值,此外,它也是提取油脂并纳入动物饲料的可行选择。
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引用次数: 1
Rabies in a previously vaccinated horse: case report 先前接种过疫苗的一匹马的狂犬病:病例报告
Q4 Veterinary Pub Date : 2022-05-20 DOI: 10.21708/avb.2022.16.2.10310
C. S. Coelho, T. D. Sodré, M. D. Freitas, L. Moroz, T. L. Nunes, T. Peixoto, P. V. Leal
Rabies is an acute, progressive, and fatal encephalomyelitis caused by a Lyssavirus. Horses affected by the disease may be a source of infection for humans. A rapid diagnosis is crucial to initiate a prompt and adequate infection control and public health measures. This manuscript reports the case of a 4-year-old gelding, 370 kg, healthy and routinely used for veterinary teaching purposes that developed rabies although vaccinated against it. Clinical signs included lameness, ataxia, muscle tremors, decubitus and pedalling, progressive paralysis, profuse salivation, teeth grinding, and whinnying. After 4 days, the animal was euthanised and definitive diagnosis was achieved through an animal inoculation test which was positive. Complementary findings included encephalomyelitis with perivascular cuffs and identification of Negri bodies in various areas of the brain.
狂犬病是由赖沙病毒引起的一种急性、进行性和致命的脑脊髓炎。受这种疾病影响的马可能是人类的感染源。快速诊断对于启动及时和充分的感染控制和公共卫生措施至关重要。这篇手稿报告了一例4岁大、体重370公斤、健康且经常用于兽医教学目的的gelding,尽管接种了狂犬病疫苗,但还是患上了狂犬病。临床症状包括跛行、共济失调、肌肉震颤、卧床不起和蹬踏、进行性麻痹、大量流涎、磨牙和呜呜声。4天后,对该动物实施安乐死,并通过阳性的动物接种试验获得最终诊断。补充研究结果包括脑脊髓炎伴血管周围套和在大脑各个区域识别内格里体。
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引用次数: 0
Main risk factors of dogs with congestive heart failure diagnosed by doppler echocardiographic parameters 多普勒超声心动图参数诊断犬充血性心力衰竭的主要危险因素
Q4 Veterinary Pub Date : 2022-05-20 DOI: 10.21708/avb.2022.16.2.10648
Bruna Pereira Nunes, Marina Gabriela Ferreira Prado, Luciene Maria Martinello Romão, F. Romão
Nowadays, congestive heart failure is one of the major complications of heart disease in small animals, which is characterized by a clinical condition in which the heart is unable to eject the blood efficiently. At long term, some compensatory mechanisms it will eventually become detrimental and, mainly, due to the congestion caused by it. In dogs, the main cause of CHF is chronic mitral valve degeneration, it is responsible for the degeneration of collagen present in the mitral valve structure that will have detrimental consequences on the entire circulatory system. The aim of the present study was to perform a retrospective study of 498 echocardiographic reports collected at a veterinary cardiology service of the Bauru region, from January 2017 to June 2019. Two groups were formed according to the Doppler echocardiographic alterations found, one that included animals with characteristics of congestive heart failure (CHF), and the other formed by non-CHF patients. Of the total number of animals evaluated, 61 were classified as affected by congestive heart failure and after classification, it was considered the main epidemiological factors (race, sex and age) of animals belonging to this group. Small breed animals were overrepresented and also elderly animals, with no apparent sexual predisposition. In addition, animals with CHF presented higher values when compared to animals without CHF of LA/Ao ratio (2.31 ± 0.30 vs. 1.46 ± 0.35), E/IRTV ratio (2.96 ± 0.39 vs. 1.16 ± 0.43) and E-wave velocity (1.45 m/s ± 0.18 vs. 0.75 m/s ± 0.22) on echocardiographic examination.
目前,充血性心力衰竭是小动物心脏病的主要并发症之一,其临床特征是心脏不能有效地排出血液。从长期来看,一些代偿机制最终会变得有害,主要是由于它造成的拥堵。在狗身上,CHF的主要原因是慢性二尖瓣变性,它是导致二尖瓣结构中胶原蛋白变性的原因,这将对整个循环系统产生有害后果。本研究的目的是对2017年1月至2019年6月在鲍鲁地区兽医心脏病学服务收集的498份超声心动图报告进行回顾性研究。根据发现的多普勒超声心动图改变组成两组,一组包括充血性心力衰竭(CHF)特征的动物,另一组由非CHF患者组成。在评估的动物总数中,有61只被分类为充血性心力衰竭,分类后认为这是属于这一组动物的主要流行病学因素(种族、性别和年龄)。小品种动物和老年动物的比例过高,没有明显的性倾向。超声心动图LA/Ao比值(2.31±0.30 vs. 1.46±0.35)、E/IRTV比值(2.96±0.39 vs. 1.16±0.43)和E波速度(1.45 m/s±0.18 vs. 0.75 m/s±0.22)均高于非CHF动物。
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引用次数: 0
Economic and production aspects of milking management in dairy farming 奶牛场挤奶管理的经济和生产方面
Q4 Veterinary Pub Date : 2022-05-20 DOI: 10.21708/avb.2022.16.2.10822
Maria Eduarda Marques Soutelino, Matheus Dias Valle, Catharine Porto Feres, Luiz Filipe Cabral de Souza Ramos, M. G. Chenard, I. M. Cunha, Marcos Antônio Aguiar Júnior, M. A. Helayel
The Brazilian dairy sector is important for the world agricultural production; however, it presents problems in production efficiency and milk quality, since the occurrence of mastitis in the herds can reach 47%. The present study analyzed data from a rural property with 100 lactating cows in a semi-intensive breeding system, which did not adopt adequate hygiene management for milking processes. Corrective measures were proposed, such as daily strip cup test, biweekly California Mastitis Test, formation of a new milking line, and daily pre dipping and post dipping and cleaning of teat cups. Milk samples from 10 animals with positive clinical mastitis, 10 animals with positive subclinical mastitis, and from the cooling tank were collected at one day before and 90 days after the implementation of the proposed measures and sent to a laboratory for analysis of somatic cell counting (SCC) and total bacteria counting (TBC). An increase of 17% in milk production and decreases of 64% and 40% in clinical and subclinical mastitis, respectively, were found after 90 days, with a consequent decrease in SCC and TBC. The milking duration after this period denoted an adaptation of the employees to the new procedures. The production financial yield was calculated based on the implementation costs of the adopted adequations and increases in milk production, representing a profitability increase of 10.4%. The implemented adequations were efficient for the control of mastitis and increase in milk yield and quality, resulting in a positive economic return.
巴西乳制品部门对世界农业生产具有重要意义;然而,它在生产效率和牛奶质量方面存在问题,因为乳腺炎在牛群中的发生率可达47%。本研究分析了一个半集约养殖系统中有100头泌乳奶牛的农村农场的数据,该系统没有对挤奶过程进行充分的卫生管理。提出了纠正措施,如每天进行条形杯测试,每两周进行一次加州乳腺炎测试,形成新的挤奶线,以及每天对乳头杯进行预浸、后浸和清洁。在拟议措施实施前一天和实施后90天,从10只临床乳腺炎阳性动物、10只亚临床乳腺病阳性动物和冷却池中采集牛奶样本,并将其送往实验室进行体细胞计数(SCC)和总细菌计数(TBC)分析。90天后,乳汁产量增加了17%,临床和亚临床乳腺炎分别减少了64%和40%,从而导致SCC和TBC减少。这段时间后的挤奶时间表明员工适应了新程序。生产财务收益是根据所采用的适当措施的实施成本和牛奶产量的增长来计算的,盈利能力增长了10.4%。所实施的适当措施有效地控制了乳腺炎,提高了牛奶产量和质量,带来了正的经济回报。
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引用次数: 0
Influence of bentonite adsorbents associated with hepatic antioxidants on the health and production of dairy cows fed diets containing naturally produced mycotoxins 与肝脏抗氧化剂结合的膨润土吸附剂对添加天然真菌毒素的奶牛健康和生产的影响
Q4 Veterinary Pub Date : 2022-05-20 DOI: 10.21708/avb.2022.16.2.10490
Dalvane Di Domenico, M. S. Oliveira, Gabriela Garbossa, G. R. Thomaz, A. C. Abreu, Carolina Rodrigues Depaoli, M. Ornaghi, O. F. Stuani, H. G. Bertagnon
As mycotoxins are consistent contaminants in the dairy cow diet, the use of adsorbents is recommended, although there are no ideal adsorbents. Although there are studies on this subject, few have focused on chronic natural intoxications. Here, we evaluated the effect of bentonite adsorbents associated with liver antioxidants on the health and milk production of dairy cows fed a diet containing naturally-produced fumonisin, zearalenone, and deoxynivalenol. Eighteen dairy cows (bodyweight 550 ±50 kg, 5 ± 2 years old, and 30 ± 1,25 kg/day milk production) in the middle of lactation were divided into groups: treatment (TG, n = 9, 22 g/day of supplement added to diet) and control (CG, n = 9, without supplement). A physical examination was performed, weekly over 56 days and blood was collected for liver and immune assessments. Milk was harvested to evaluate milk production and content (fat protein, somatic cell count, and lactose). The additive promoted beneficial effects on the liver from the 24th day due to a decrease in the enzymatic activities of gamma-glutamyltransferase and lactate dehydrogenase and increased serum protein and albumin levels. There were improvements in health, evidenced as fewer clinical manifestations of the disease, greater leukocyte oxidative metabolism capacity, and a lower neutrophil lymphocytes ratio. The treatment also promoted a 19% increase in milk volume. It was concluded that the additive promoted health benefits and milk production in dairy cows.
由于真菌毒素是奶牛饮食中的一贯污染物,尽管没有理想的吸附剂,但建议使用吸附剂。尽管有关于这一主题的研究,但很少有人关注慢性自然中毒。在这里,我们评估了与肝脏抗氧化剂相关的膨润土吸附剂对饲喂含有天然产生的伏马菌素、玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇的奶牛的健康和产奶的影响。将18头泌乳中期奶牛(体重550±50kg,5±2岁,产奶量30±1.25 kg/d)分为治疗组(TG,n=9,日粮中添加22g/d补充剂)和对照组(CG,n=9;不添加补充剂)。在56天内每周进行一次体检,并采集血液进行肝脏和免疫评估。采集牛奶以评估牛奶产量和含量(脂肪蛋白、体细胞计数和乳糖)。从第24天起,由于γ-谷氨酰转移酶和乳酸脱氢酶的酶活性降低以及血清蛋白和白蛋白水平升高,添加剂促进了对肝脏的有益作用。健康状况有所改善,表现为该疾病的临床表现更少,白细胞氧化代谢能力更强,中性粒细胞-淋巴细胞比例更低。这种治疗也促进了19%的奶量增加。结果表明,该添加剂能促进奶牛的健康和产奶。
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引用次数: 0
Vaginal cytology in mares in the different stages of the estral cycle associated with the seasons of the year 与一年中的季节有关的不同发情周期阶段的母马阴道细胞学
Q4 Veterinary Pub Date : 2022-05-20 DOI: 10.21708/avb.2022.16.2.10342
Joice Maria Bazerla Andreta, Luiz Henrique Garcia da Silva, Matheus Runyan Belotto, C. M. Mansano, H. A. Netto, Anelise Ribeiro Peres
The reproductive biotechnologies directly assist in the development of different methods of reproductive evaluation. The objective of this study was to develop a new method to determine the estrous cycle phase in equine females through vaginal cytology throughout the seasons, as well as to establish an estimate of inflammatory cells present in the vagina. Six mares were evaluated at different ages without a defined breed and reproductive activity and were subjected to ultrasound evaluation of the reproductive system and cytological analysis of the vaginal region. In the summer, there was a predominance of keratinized epithelial cells in the estrus and intermediate in the diestrus. In the autumn, there was a predominance of keratinized cells in estrus and parabasal cells in estrus and diestrus. In anestrus (winter), a greater number of parabasal cells was identified in relation to the other cell types. In estrus (spring), there was a predominance of parabasal and intermediate cells. Conversely, in diestrus, parabasals were found in greater numbers. The evaluation of inflammatory cells of the vaginal epithelium of mares showed greater activity in the summer; however, there are no reference values for healthy animals in the literature, and it is necessary to conduct studies on the subject. It is concluded that there is an influence of cyclicity on the vaginal epithelium of the equine species, varying according to the season. Additionally, vaginal cytological evaluation is an important complementary tool in the diagnosis of vaginitis in mares, requiring further research on the subject.
生殖生物技术直接有助于开发不同的生殖评估方法。本研究的目的是开发一种新的方法,通过整个季节的阴道细胞学来确定马雌性的发情周期阶段,并对阴道中存在的炎症细胞进行评估。在没有明确品种和繁殖活动的情况下,对六匹不同年龄的母马进行了评估,并对其生殖系统进行了超声波评估和阴道区域的细胞学分析。在夏季,角质化上皮细胞在发情期占主导地位,在发情期处于中间地位。秋季发情期以角质化细胞为主,发情期和发情期以基底旁细胞为主。在麻醉期(冬季),与其他细胞类型相比,发现了更多的鼻旁细胞。在发情期(春季),基底旁细胞和中间细胞占优势。相反,在间挤中,发现对羟基苯甲酸酯的数量更多。对母马阴道上皮炎性细胞的评估显示,夏季有更大的活性;然而,文献中没有对健康动物的参考价值,有必要对其进行研究。得出的结论是,周期性对马的阴道上皮有影响,因季节而异。此外,阴道细胞学评估是诊断母马阴道炎的一个重要补充工具,需要进一步研究。
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Acta Veterinaria Brasilica
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