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Advances in Understanding the Pathogenesis: Bacterial Surface Glycans as The Virulence Factor and Role in Pathogen Bacteria 致病机制的研究进展:细菌表面聚糖作为毒力因子及其在致病菌中的作用
Q4 Multidisciplinary Pub Date : 2023-08-18 DOI: 10.32802/asmscj.2023.1235
C. Ghazaei, S. Amjad
Bacterial glycans are essential components of bacterial walls and crucially important cell surface antigens such as lipopolysaccharide and polysaccharide capsules, which act as virulence-causing factors. Polysaccharide capsules in bacteria are naturally composed of recurring oligosaccharides and lipopolysaccharides. LPS is composed of domains that are hydrophobic in nature known as lipid A or endotoxin, which is a nonrepeating oligosaccharide "core", and an O-antigen. Interaction of glycan-receptor is critical in pattern recognition of microorganisms and regulatory signals that perform regular immune cell activities. The true glycan structure is important for collaboration with the immune system of host, as demonstrated by microorganisms that produce polycarbohydrates that mimic host particles in order to evade the immunity of host. Certain bacteria circumvent antibody defences by molecularly mimicking the glycan structures of host and masquerade as "self" to avoid identification by immune system. Phages are the most prevalent biological entities on our planet, and they evolved glycan-binding proteins (GBPs) to bind to bacterial glycans. These proteins are in charge of the breakdown of antibodies and are impervious to harsh conditions where bacteria live for example, the gut of mammals, water, and soil. In order to increase contact with bacterial glycans, the phages manufacture multimeric proteins.
细菌聚糖是细菌壁的重要成分,也是至关重要的细胞表面抗原,如脂多糖和多糖胶囊,它们是致病因子。细菌中的多糖胶囊是由反复出现的低聚糖和脂多糖组成的。LPS由本质上被称为脂质A或内毒素的疏水结构域和O-抗原组成,脂质A或脂多糖是一种不重复的寡糖“核心”。聚糖受体的相互作用在微生物的模式识别和执行常规免疫细胞活动的调节信号中至关重要。真正的聚糖结构对于与宿主免疫系统的合作很重要,正如微生物所证明的那样,这些微生物产生模仿宿主颗粒的聚碳水化合物,以逃避宿主的免疫。某些细菌通过分子模拟宿主的聚糖结构来规避抗体防御,并伪装成“自我”以避免被免疫系统识别。噬菌体是我们星球上最普遍的生物实体,它们进化出聚糖结合蛋白(GBP)与细菌聚糖结合。这些蛋白质负责抗体的分解,不受细菌生活的恶劣条件的影响,例如哺乳动物的肠道、水和土壤。为了增加与细菌聚糖的接触,噬菌体制造多聚体蛋白质。
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引用次数: 0
Modelling Backscattered Light in Fibre Optic Based on Light Propagation 基于光传播的光纤中背散射光建模
Q4 Multidisciplinary Pub Date : 2023-08-17 DOI: 10.32802/asmscj.2023.1353
W. Z. Wan Ismail
Nowadays, fibre optic sensors have been widely used to detect temperature, pressure, vibration and other physical properties. To design high performance fibre optics sensors, sensing elements such as light propagation need to be investigated for parameters such as absorbance, transmittance and scattering. In this study, the backscattered light is studied and modelled using MATLAB based on Brillouin and Rayleigh scattering. The scattering effects on the output power and Stokes power are clearly seen where both powers reduce with the increase of fibre length. The Rayleigh power in terms of Coherent Rayleigh Noise is studied where broader source linewidth, higher spatial resolution and lower group velocity produce less noise. The less fluctuation due to noise can be attributed to the less temporal and spatial fluctuations of the optical pump power or pump current. The output from this research can benefit experimental studies to design fibre optic sensors in future.
目前,光纤传感器已广泛应用于温度、压力、振动等物理性能的检测。为了设计高性能光纤传感器,需要研究光传播等传感元件的吸光度、透射率和散射等参数。本文利用MATLAB对基于布里渊散射和瑞利散射的后向散射光进行了研究和建模。当输出功率和斯托克斯功率随光纤长度的增加而减小时,散射效应明显。从相干瑞利噪声的角度研究了较宽的源线宽、较高的空间分辨率和较低的群速对相干瑞利噪声的影响。由噪声引起的波动较小可归因于光泵浦功率或泵浦电流的时间和空间波动较小。本研究成果可为未来光纤传感器的设计提供实验依据。
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引用次数: 0
A Review of Educators’ Experiences in Conducting Emergency Remote Teaching During Pandemic COVID-19 新冠肺炎疫情期间教育工作者开展应急远程教学的经验回顾
Q4 Multidisciplinary Pub Date : 2023-08-16 DOI: 10.32802/asmscj.2023.1275
Norasykin Mohd Zaid
The pandemic of COVID-19 has dreadfully forced a global shut down of educational activities and caused a crisis-response where schools and institutions prompted online learning to serve as the alternative educational platform. The migration from face-to-face teaching into an online system had led to a tremendous problem since most educators had little or no training at all. Thus, this study aimed to produce a review of educators’ experiences in conducting emergency remote teaching during pandemic. This paper presented a review of empirical studies published between January to December 2020. A systematic search was conducted in Scopus. The inclusion criteria were primary studies of teachers’ experiences in conducting emergency remote teaching (ERT). The included studies were reviewed based on their methodology, setting and population, scopes of study and thematic coding of teachers’ experiences. The findings of this study indicated that there was room for improvement in teachers’ profession and online instructional models to prepare for the unknown incoming crisis.
新冠肺炎的大流行可怕地迫使全球关闭教育活动,并引发了危机响应,学校和机构促使在线学习成为替代教育平台。从面对面教学迁移到在线系统导致了一个巨大的问题,因为大多数教育工作者几乎没有或根本没有受过培训。因此,本研究旨在回顾教育工作者在疫情期间进行紧急远程教学的经验。本文回顾了2020年1月至12月期间发表的实证研究。在Scopus进行了系统的搜索。纳入标准是对教师进行紧急远程教学(ERT)经验的初步研究。纳入的研究根据其方法、背景和人群、研究范围和教师经验的主题编码进行了审查。这项研究的结果表明,教师的职业和在线教学模式还有改进的空间,以应对未知的危机。
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引用次数: 0
Recovery of Pulp from Oil Palm Empty Fruit Bunch and Used Paper for Sustainable Paper Production 从油棕空果包和废纸中回收纸浆用于可持续造纸
Q4 Multidisciplinary Pub Date : 2023-08-16 DOI: 10.32802/asmscj.2023.1419
H. L. Tan, Y. P. Lim, Nadia Syakirah Mohd Nahar, M. S. So'aib, N. F. Abu Bakar
Agricultural waste residue such as empty fruit bunch (EFB) has great potential as an alternative feedstock in pulp and paper industry. This study provides insights into the use of EFB as an alternative non-wood fibre resource for pulping and papermaking. EFB was mixed with used paper (UP) according to the ratio of 100:0, 75:25, 50:50, 25:75 on dry basis. The hemi-cellulose and lignin contents in EFB was removed by soda-pulping using 15% w/v sodium hydroxide for 30 minutes at 100°C. Chemical analyses were performed to determine the ash content, lignin content, and the cellulose content (Rowel method). Physical and mechanical properties of the EFB/UP paper were characterised according to the TAPPI Standards including grammage, porosity, moisture content and tensile strength. The average fibre length of all EFB/UP papers showed different fibre length ranging from 1.0 to 1.2 mm. EFB25 (25% EFB) was found to have the least moisture content of 7.28% meanwhile EFB100 had the least ash content of 2.28% and the highest cellulose and lignin content of 45.22% and 26.5%, respectively. EFB25 demonstrated the highest tensile index of 2.01 Nm/g. There are no major colour changes and no trace of fungal growth on the surface of all paper samples after 4 weeks of storage without temperature and humidity control. It was found that paper with EFB25 displayed good physical appearance with grammage of 59.8 g/m2 that is almost comparable to commercialised paper of 70 g/m2 and suitable for writing.
空果穗等农业废渣作为制浆造纸工业的替代原料具有很大的潜力。这项研究为EFB作为制浆造纸的替代非木材纤维资源的使用提供了见解。将EFB与废纸(UP)按干基100:0、75:25、50:50、25:75的比例混合。EFB中的半纤维素和木质素含量通过在100°C下使用15%w/v氢氧化钠进行30分钟的苏打制浆来去除。进行化学分析以确定灰分含量、木质素含量和纤维素含量(罗威尔法)。EFB/UP纸的物理和机械性能根据TAPPI标准进行表征,包括克重、孔隙率、含水量和拉伸强度。所有EFB/UP纸的平均纤维长度显示出不同的纤维长度,从1.0到1.2mm不等。发现EFB25(25%EFB)的含水量最低,为7.28%,而EFB100的灰分最低,为2.28%,纤维素和木质素含量最高,分别为45.22%和26.5%。EFB25表现出2.01牛米/克的最高拉伸指数。在没有温度和湿度控制的情况下储存4周后,所有纸张样品的表面没有重大颜色变化,也没有真菌生长的痕迹。发现具有EFB25的纸显示出良好的物理外观,克重为59.8g/m2,其几乎可与70g/m2的商业化纸相媲美并且适合书写。
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引用次数: 0
Virtual Reality as Experiential Learning to Promote STEM-DRR in Tertiary Education 虚拟现实作为体验式学习促进STEM-DRR在高等教育中的应用
Q4 Multidisciplinary Pub Date : 2023-08-16 DOI: 10.32802/asmscj.2023.1370
M. Anggaryani, T. Prastowo, N. Suprapto, Jonatan A. Lassa, M. Madlazim, F. A. Alifteria, A. I. Agusty, Nurita Apridiana Lestari
Efforts and discourses of the need to integrate disaster education into a science curriculum for university students in Indonesia are well documented. However, lecturers need more room to introduce Disaster Risk Reduction (DRR) in a way that involves students as experiential learners. Integrating DRR into Science, Technology, Engineering, and Mathematics (STEM) subjects is a crucial point. The current study used virtual reality (VR) as a pedagogical tool for learning through experience to promote STEM-DRR at tertiary levels of education. This experimental study aimed to determine the effects of VR use on students' experiential learning based on the VR online training developed activities. The methods for data collection included observation and survey. Ten participants were in the final year of their study and were recruited based on their interest in using VR media for their thesis. The results showed that half of the participants understood the training content, and 40% were classified as knowledgeable before training. In addition, significant contributions to the learning process were observed in training, where half participants were satisfied with the new skills attained, and 80% of the participants argued that the training was beneficial to their final projects. Thus, in the present study integrating STEM-DRR into the VR training content to promote students' awareness of disaster risk is considered successful. In conclusion, the results suggest that technology-enhanced learning supports distance learning and is proven economical and efficient.
关于有必要将灾害教育纳入印度尼西亚大学生科学课程的努力和论述都有很好的记录。然而,讲师需要更多的空间来介绍减少灾害风险(DRR),让学生成为体验式学习者。将DRR融入科学、技术、工程和数学(STEM)科目是一个关键点。目前的研究使用虚拟现实(VR)作为一种通过经验学习的教学工具,在高等教育中推广STEM-DRR。本实验研究旨在基于虚拟现实在线培训开发的活动,确定虚拟现实的使用对学生体验式学习的影响。数据收集的方法包括观察和调查。十名参与者正处于研究的最后一年,他们是基于对在论文中使用VR媒体的兴趣而被招募的。结果显示,一半的参与者了解培训内容,40%的参与者在培训前被归类为知识渊博。此外,在培训中观察到对学习过程的重大贡献,一半的参与者对所获得的新技能感到满意,80%的参与者认为培训对他们的最终项目有益。因此,在本研究中,将STEM-DRR整合到VR培训内容中,以提高学生的灾害风险意识被认为是成功的。总之,研究结果表明,技术强化学习支持远程学习,并被证明是经济有效的。
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引用次数: 1
Optimisation of Fungal Laccase Production from Monodictys castaneae castaneae真菌产漆酶的优化
Q4 Multidisciplinary Pub Date : 2023-08-14 DOI: 10.32802/asmscj.2023.1369
Abdelrahman Mohamed Tammam
Laccases are copper-containing oxidase enzymes that have broad substrate specificity and applicability in industrial processes. The purpose of this work was to optimise laccase production by a certain endophytic fungus. Monodictys castaneae (Wallr.) Hughes was cultured and incubated using different nutritional and physiological factors affecting laccase production. It was found that the optimum physiological conditions for laccase production were 4 disc inoculum size, a 9-day fermentation period, pH 5, and 28°C. The enzyme was purified using acetone precipitation, gel filtration, and ion-exchange chromatography. The enzyme was identified as a monomeric protein with a molecular mass of 63 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Different concentrations of CuSO4 were used to stimulate laccase production and activity. A 0.15 g/l concentration of CuSO4 was the optimum dosage for laccase activity enhancement. For enzyme-guaiacol interaction, the optimal pH and temperature were 5.4 and 35 °C, respectively. The laccase activity was highly stimulated using 3 mM and 7 mM concentrations of chloride salts of Cd+2, Ca+2, Cr+2 and Fe+3, but the enzyme activity was slightly inhibited by 10 mM concentrations of these metal ions. According to all previous results, Monodictys castaneae showed promising potential as a producer of laccase.
漆酶是一种含铜氧化酶,在工业生产过程中具有广泛的底物特异性和适用性。本研究的目的是对某内生真菌产漆酶进行优化。牡丹属植物利用影响漆酶产生的不同营养和生理因素培养和孵育Hughes。结果表明,产漆酶的最佳生理条件为4盘接种量、发酵时间9 d、pH 5、28℃。采用丙酮沉淀法、凝胶过滤法和离子交换色谱法纯化酶。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳鉴定为分子量为63 kDa的单体蛋白。用不同浓度的CuSO4刺激漆酶的产生和活性。0.15 g/l的CuSO4浓度是提高漆酶活性的最佳剂量。酶-愈创木酚互作的最佳pH和温度分别为5.4℃和35℃。3 mM和7 mM浓度的Cd+2、Ca+2、Cr+2和Fe+3氯化物对漆酶活性有较高的刺激作用,10 mM浓度的这些金属离子对漆酶活性有轻微抑制作用。根据以往的研究结果,castaneae作为漆酶的生产者具有很大的潜力。
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引用次数: 0
Engineer 5G to Transform Healthcare Industry 设计5G,改变医疗行业
Q4 Multidisciplinary Pub Date : 2023-08-03 DOI: 10.32802/asmscj.2023.1339
Zhang Jing Bing
Abstract— 5G is the fifth-generation innovation standard for broadband cell organizations, which wireless organizations started conveying worldwide in 2019, and is the arranged replacement to the 4G networks which give network to most current cellphones. 5G networks are anticipated to have more than 1.7 billion supporters worldwide by 2025. 5G has been developing and changing the medical care industry, and a great deal of it is legitimized. 5G can convey gigabit speeds, inertness under 10 milliseconds, expanded inclusion, and an immense limit of as much as 1 million gadgets for each organization. In general, when we got sick and needed clinical consideration, we might have had the option of going to a specialist or a clinic.
摘要——5G是宽带蜂窝组织的第五代创新标准,无线组织于2019年开始在全球范围内推广,是为大多数当前手机提供网络的4G网络的安排替代品。预计到2025年,5G网络将在全球拥有超过17亿支持者。5G一直在发展和改变医疗保健行业,其中很大一部分是合法的。5G可以传输千兆比特的速度、10毫秒以下的惰性、扩大的包容性,以及每个组织多达100万个小工具的巨大限制。一般来说,当我们生病需要临床考虑时,我们可能可以选择去专科医生或诊所。
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引用次数: 1
Fear and Disgust Toward Insect Among Psychiatric Disorder Patients and Healthy People 精神障碍患者和健康人对昆虫的恐惧和厌恶
Q4 Multidisciplinary Pub Date : 2023-07-31 DOI: 10.32802/asmscj.2023.1428
S. Sharip, A. Azil, N. Hassan, Z. Sharip
The present study investigates the prevalence of fear and disgust toward insects among people with mental illness. A cross-sectional survey using a newly designed arthropods survey was distributed to targeted population using both google forms and face-to-face recruitment. Out of 175 respondents in the study, 41% are diagnosed with any psychiatric disorder while 59% are healthy people. There are significant differences between fear and disgust on insects among healthy people and people with mental illness. Wasp (87.3%), weaver ant (44.4%), and sago larvae (31.3%) were feared by psychiatric patients. Sago larvae was the most disgusting insect by people with mental illness (51.5%) while healthy people were more disgusted with caterpillars (53.4%). The fear and disgust of different insects vary with gender and employment among the two groups; female from both groups were more disgusted with head lice than male while employed healthy adults feared head lice. This study provides new knowledge on the existence of fear and disgust of arthropods among people with mental illness. Since such people have high comorbidity with other psychiatric disorders, this finding provides new insights on the prevalence of fear and disgust toward insects among the patients for early intervention by health professionals.
本研究调查了精神病患者对昆虫的恐惧和厌恶的普遍程度。采用新设计的节肢动物调查进行横断面调查,采用谷歌表格和面对面招募的方式对目标人群进行调查。在这项研究的175名受访者中,41%被诊断患有任何精神疾病,而59%是健康人。健康人群和精神疾病人群对昆虫的恐惧和厌恶存在显著差异。精神病患者对黄蜂(87.3%)、编织蚁(44.4%)和西米幼虫(31.3%)最为恐惧。精神疾病人群最厌恶西米幼虫(51.5%),健康人群最厌恶毛虫(53.4%)。两组人对不同昆虫的恐惧和厌恶程度因性别和职业而异;两组女性对头虱的厌恶程度都高于男性,而受雇的健康成年人对头虱的恐惧程度则高于男性。本研究为精神疾病患者对节肢动物的恐惧和厌恶的存在提供了新的认识。由于这些人与其他精神疾病有很高的合并症,这一发现为卫生专业人员早期干预患者对昆虫的恐惧和厌恶的普遍程度提供了新的见解。
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引用次数: 0
Analysis of ULF Emissions in Solar Winds as A Short-Term Earthquake Precursor 作为短期地震前兆的太阳风ULF发射分析
Q4 Multidisciplinary Pub Date : 2023-07-18 DOI: 10.32802/asmscj.2023.1189
Roslan Umar, S. S. Zafar, N. Sabri, M. H. Jusoh, S. Abe, A. Yoshikawa
The physical destruction and fatalities caused by earthquake events compelled scientists to develop Earthquake predictions. Due to limited seismometer sensitivity, it was impossible to detect earthquake events; therefore, non-seismological is established, which in Ultra Low Frequency (ULF). The study of electromagnetic waves (EM) in the Ultra-Low Frequency (ULF) range is a promising tool for investigating seismomagnetic effects that act as an earthquake precursor. This study analysed the ULF frequency range as a short-term earthquake precursor at a depth < 100 km and an epicentral distance < 100 km (distance from Cebu magnetometer station) measured using a ground magnetometer installed in Cebu (10.36oN, 123.91oE), in the Philippines. This study also intended to determine the emission of magnetic pulsation (Pc4 and Pc5), solar wind parameters and near equatorial geomagnetic storms (SYM/H) in low latitude regions prior to an earthquake event. Findings show that the most evident ULF that acts as a potential earthquake precursor was at a frequency range of Pc5 (1.7 - 6.7 mHz) compared to Pc4 (6.7 - 22 mHz).  It also shows that high solar wind changes and geomagnetic storms respond to the emission of ULF magnetic pulsations (Pc4 and Pc5) prior to earthquake events at low latitudes.  Thus, it can be concluded that magnetic pulsations are signatures that indicate the probability of short-term earthquakes precursor.
地震事件造成的物质破坏和人员伤亡迫使科学家们发展地震预测。由于地震仪的灵敏度有限,不可能探测到地震事件;因此,建立了在超低频率(ULF)下的非地震学。研究超低频(ULF)范围内的电磁波(EM)是研究作为地震前兆的地震磁效应的一种很有前途的工具。本研究分析了在深度< 100公里和震中距离< 100公里(距宿务磁力计站的距离)的短期地震前兆ULF频率范围,使用安装在菲律宾宿务(10.36oN, 123.91东经)的地面磁力计测量。本研究还旨在确定低纬度地区在地震发生前的磁脉动(Pc4和Pc5)发射、太阳风参数和近赤道地磁风暴(SYM/H)。研究结果表明,与Pc4 (6.7 - 22 mHz)相比,Pc5 (1.7 - 6.7 mHz)的频率范围是最明显的潜在地震前兆。在低纬度地区,高纬度太阳风变化和地磁风暴对超低频磁脉冲(Pc4和Pc5)的发射有响应。因此,可以得出结论,磁脉动是指示短期地震前兆概率的特征。
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引用次数: 0
Optimisation of Edible Coating to Improve the Postharvest Shelf-life of Guava Using Response Surface Methodology 响应面法优化番石榴食用包衣提高其采后保质期
Q4 Multidisciplinary Pub Date : 2023-07-13 DOI: 10.32802/asmscj.2023.1520
Tingxing Hua, M. Z. Abidin, M. S. Abdullah, A. Razak, Mohammad Hafsanjani Salleh, Siti Nadhira Mohamad Basri
The effects of composite edible coating consisting of gum Arabic, beeswax and coconut oil on Cambodia cultivar guava (Psidium guajava) were evaluated using response surface methodology (RSM). The optimum coating formulation was determined using the weight loss percentage and total soluble solid (TSS) content as the responses. From the central composite rotatable design in RSM, a total of 15 sets of coating compositions were designed. The optimised coating emulsion was made up of gum Arabic (6.6% w/v), beeswax (5.5% w/v) and coconut oil (3.6% w/v) with Tween 80 (3% w/v) as surfactant. Under this optimised coating emulsion, the predicted mean values of weight loss and TSS were 2.91% and 7.84oBrix respectively, whereas the actual mean values of guava samples were 3.38% and 8.00oBrix respectively, after 6 days of ambient storage. The actual mean values of both responses were within the 95% prediction interval, which was 2.35 – 3.47% for weight loss and 7.22 – 8.45oBrix for TSS. The optimised coating emulsion successfully reduced weight loss with maximised TSS content for guava.
采用响应面法(RSM)评价了阿拉伯树胶、蜂蜡和椰子油复合食用涂层对柬埔寨番石榴(Psidium guajava)的影响。使用重量损失百分比和总可溶性固体(TSS)含量作为响应来确定最佳涂料配方。从RSM的中心复合材料可旋转设计出发,共设计了15套涂料组合物。优化的涂层乳液由阿拉伯树胶(6.6%w/v)、蜂蜡(5.5%w/v)和椰子油(3.6%w/v)组成,吐温80(3%w/v)作为表面活性剂。在这种优化的涂层乳液下,环境储存6天后,重量损失和TSS的预测平均值分别为2.91%和7.84oBrix,而番石榴样品的实际平均值分别是3.38%和8.00oBrix。两种反应的实际平均值都在95%的预测区间内,体重减轻为2.35–3.47%,TSS为7.22–8.45oBrix。优化的涂层乳液成功地减少了重量损失,最大限度地提高了番石榴的TSS含量。
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引用次数: 0
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