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Comparative evaluation of pericervical dentin preservation and fracture resistance of root canal-treated teeth with rotary endodontic file systems of different types of taper - An in vitro study. 不同锥度的旋转根管锉系统对根管治疗牙齿的颈周牙本质保存和抗折性的比较评价——一项体外研究。
Q2 Dentistry Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/jcd.jcd_255_23
P Shyma, Josey Mathew, Liza George, R V Vineet, Sinju Paul, Aleesha Joy

Aims: The aim of this study was to evaluate and compare the pericervical dentin preservation and fracture resistance of root canal-treated teeth with rotary endodontic file systems of different types of taper.

Subjects and methods: Thirty-two single-rooted human-extracted premolars were used. They were mounted in wax, and preoperative cone-beam computed tomography (CBCT) scans were taken with 11 × 8 Field of view (FOV). The evaluation of the pericervical dentin thickness was done at the cementoenamel junction level. After pre-CBCT, the 32 samples were divided into four groups (n = 8) - Group A: fixed tapered hand files, Group B: variable regressive tapered TruNatomy, Group C: progressive tapered ProTaper Gold, and Group D: fixed tapered HyFlex EDM. Following instrumentation, postoperative CBCT scans were taken to evaluate pericervical dentin thickness. Obturation was done and access cavity was restored with composite. Fracture strength was checked for all the samples using "universal testing machine" until fracture, and calculated in newtons (N).

Statistical analysis used: Student's t-test and ANOVA test, along with Tukey's post hoc analysis, were used for comparing mean values between the groups, and P < 0.05 was considered statistically significant.

Results: The results of this study showed that there is no statistically significant difference in preserving pericervical dentin with file system of different types of taper and fracture resistance between the groups.

Conclusions: Within the limitations of the study, it was concluded that different types of file taper systems used for root canal preparation have no significant effect on the preservation of pericervical dentin and fracture resistance of teeth.

目的:本研究的目的是评估和比较不同锥度类型的旋转根管锉系统对根管治疗牙齿的颈周牙本质保存和抗折性。受试者和方法:使用32颗单根人类拔除的前磨牙。他们被安装在蜡中,术前用11×8视场(FOV)进行锥束计算机断层扫描(CBCT)。在牙骨质-牙本质连接水平上评估颈周牙本质厚度。预CBCT后,将32个样本分为四组(n=8)——A组:固定锥形手动锉,B组:可变回归锥形TruNatomy,C组:渐进锥形ProTaper Gold,D组:固定楔形HyFlex EDM。器械植入后,进行术后CBCT扫描以评估颈周牙本质厚度。完成了闭塞,并用复合材料修复了进入腔。使用“万能试验机”检查所有样本的断裂强度,直到断裂,并以牛顿(N)为单位进行计算。使用的统计分析:使用Student t检验和ANOVA检验以及Tukey的事后分析来比较各组之间的平均值,P<0.05被认为具有统计学意义。结果:本研究结果表明,不同锥度和抗折性的锉刀系统在保存颈周牙本质方面,两组之间没有统计学上的显著差异。结论:在本研究的范围内,得出的结论是,用于根管预备的不同类型的锉削系统对保存颈周牙本质和牙齿的抗骨折性没有显著影响。
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引用次数: 0
Life span of Neoendo Flex and ProTaper Next rotary files with reciprocating motion in single-rooted teeth. Neoendo Flex和ProTaper Next旋转锉的使用寿命,可在单根牙齿中进行往复运动。
Q2 Dentistry Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/jcd.jcd_239_23
Ajay Chhabra, K P Ramya, B Saravana Prathap, Priyanka Yadav

Background: Cleaning and shaping represent a vital step in the endodontic procedure. In routine endodontic therapy, the fracture of nickel-titanium (NiTi) instruments is a procedural problem creating a major obstacle to therapy.

Aim: This study examines the life span of one Neoendo Flex and ProTaper Next (PTN) rotary file using reciprocating motion and compares the time required by both file systems for canal preparation.

Materials and methods: One hundred maxillary permanent central incisors were selected. In that, fifty teeth are utilized in a reciprocating motion (RM) with the PTN file (n = 25) and Neoendo Flex file (n = 25). To check fractured resistance and the overall root canal preparation time, fifty more teeth are employed in continuous motion (CM) with canals prepared using PTN (n = 25) and Neoendo Flex (n = 25). All canals are prepared following the sequence of respective files. Later, the fracture mechanism of the files was inspected using a scanning electron microscope.

Results: In an RM motion, both PTN and Neoendo Flex files can be used in a minimum of 25 canals in single-rooted teeth. Statistical analysis using the Mann-Whitney U-test showed no significant difference in total time taken by PTN and Neoendo Flex in both motions.

Conclusion: Within the limitation of this study, the RM was found to be better than CM with less incidence of instrument fracture.

背景:清洁和整形是根管治疗过程中至关重要的一步。在常规牙髓治疗中,镍钛(NiTi)器械的骨折是一个程序性问题,是治疗的主要障碍。目的:本研究考察了一种使用往复运动的Neoendo Flex和ProTaper Next(PTN)旋转锉的使用寿命,并比较了两种锉系统进行管预备所需的时间。材料与方法:选取100颗上颌恒中切牙。其中,在具有PTN锉刀(n=25)和Neoendo-Flex锉刀(n=25)的往复运动(RM)中使用50个齿。为了检查断裂阻力和整个根管预备时间,再使用50颗牙齿进行连续运动(CM),使用PTN(n=25)和Neoendo-Flex(n=25%)预备根管。所有水渠均按照各自文件的顺序进行准备。随后,使用扫描电子显微镜检查了锉刀的断裂机制。结果:在RM运动中,PTN和Neoendo-Flex锉都可以在单根牙齿的至少25个根管中使用。使用Mann-Whitney U型检验的统计分析显示,PTN和Neoendo-Flex在两种运动中花费的总时间没有显著差异。结论:在本研究的范围内,RM优于CM,器械骨折发生率较低。
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引用次数: 0
Comparative assessment of the outcome of complete pulpotomy using mineral trioxide aggregate and Biodentine in mature permanent mandibular molars with symptomatic irreversible pulpitis: A randomized clinical trial with 18 months of follow-up. 使用矿物三氧化二聚集体和生物牙本质对患有症状性不可逆牙髓炎的成熟下颌恒磨牙进行完全牙髓切断术结果的比较评估:一项为期18个月随访的随机临床试验。
Q2 Dentistry Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/jcd.jcd_170_23
Rakesh Singla, Vipula Laller, Gurdeep Singh Gill, Namita Jain, Tarun Kumar, Jaidev Singh Dhillon

Background: Mature permanent teeth with irreversible pulpitis have traditionally been managed with pulpectomy. With advancements in pulp biology and dental materials, many clinicians are using vital pulp therapies like pulpotomy to manage such teeth. The current study was conducted to help clinicians in making decisions about case selection and choice of material for such cases.

Aim: This randomized clinical trial evaluated the outcome of complete pulpotomy, using mineral trioxide aggregate (MTA) and Biodentine, in permanent mandibular molars with symptomatic irreversible pulpitis (SIP).

Materials and methods: Fifty patients with moderate-to-severe pain in mandibular molars with SIP were included in this prospective, parallel, single-blind clinical trial. Coronal pulp was completely removed and hemostasis was achieved with a cotton pellet moistened with 2.5% sodium hypochlorite. Subsequently, the radicular orifices were randomly covered with MTA or Biodentine. All teeth were permanently restored with composite restoration at the same appointment. Clinical evaluation was performed at 1 week, 3 months, 6 months, 12 months, and 18 months and radiographic evaluation was done after 6 months, 12 months, and 18 months. Mann-Whitney U and Chi-square tests were utilized for statistical analysis.

Results: Success rates of MTA and Biodentine pulpotomy were 63.6% and 69.6%, respectively, with no significant difference between the two groups at any follow-up period (P > 0.05).

Conclusion: There were no significant differences in complete pulpotomy success rates between MTA and Biodentine over 18 months in mandibular molars with SIP.

背景:患有不可逆牙髓炎的成熟恒牙传统上采用牙髓切除术治疗。随着牙髓生物学和牙科材料的进步,许多临床医生正在使用至关重要的牙髓疗法,如牙髓切开术来管理这些牙齿。目前的研究旨在帮助临床医生做出病例选择和此类病例材料选择的决定。目的:这项随机临床试验评估了使用矿物三氧化物聚集体(MTA)和生物牙本质对有症状的不可逆牙髓炎(SIP)的下颌恒磨牙进行完全牙髓切断术的结果。材料和方法:50名患有SIP的下颌磨牙中度至重度疼痛的患者被纳入这项前瞻性、平行、单盲临床试验。冠状髓被完全去除,并用2.5%次氯酸钠润湿的棉粒止血。随后,用MTA或生物牙本质随机覆盖根孔。所有牙齿均在同一时间进行了复合修复。在1周、3个月、6个月、12个月和18个月时进行临床评估,在6个月和12个月后进行放射学评估。Mann-Whitney U和卡方检验用于统计分析。结果:MTA和生物牙本质牙髓切断术的成功率分别为63.6%和69.6%,两组在任何随访期均无显著差异(P>0.05)。
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引用次数: 0
Effect of remineralizing agent on laser and nonlaser bleached enamel surfaces subjected to erosion: An in vitro study. 再矿化剂对激光和非激光漂白搪瓷表面侵蚀的影响:体外研究。
Q2 Dentistry Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/jcd.jcd_139_23
Swapnil Vats, Dakshita Joy Sinha, Sarita Singh, Ruchi Rathi, Swati Jha, Isha Singh

Aim: Assessment of remineralizing agent's effect on laser and nonlaser bleached enamel surfaces subjected to erosion.

Materials and methods: In this study, 80 extracted human permanent anteriors were collected and divided into 4 groups with 20 teeth per sample. Enamel specimen of 3 mm × 3 mm were made using polyvinyl chloride rings and acrylic and randomly divided into four groups. Then the specimens were subjected to initial microhardness test using Vicker's hardness tester (AVK-CO, Mitutoyo, Japan). Two indentations were placed at 100 mm from one another in the center of all the samples. Bleaching with laser and without laser using hydrogen peroxide was performed followed by remineralization in the groups (Groups 1a and 2a) and then were subjected to erosion. The final hardness was measured using the above method used for initial microhardness.

Results: Microhardness in the remineralized groups showed least variation. The group in which laser bleaching was performed along with remineralization as compared with nonlaser bleaching group with remineralization showed improvement in microhardness but the data was not statistically significant. A significant difference was noted between the laser and remineralization group when compared with the groups in which no remineralization was done.

Conclusion: After bleaching the enamel surface is more prone to erosion, so to improve the microhardness of bleached enamel a remineralizing agent should be used. A combination of diode laser bleaching and remineralizing agents leads to improved microhardness of the bleached enamel thus proving this combination to be efficacious.

目的:评估再矿化剂对激光和非激光漂白搪瓷表面侵蚀的影响。材料和方法:本研究收集了80颗提取的人类恒牙前部,并将其分为4组,每组20颗。用聚氯乙烯环和丙烯酸制成3mm×3mm的搪瓷试样,并随机分为四组。然后使用Vicker硬度计(AVK-CO,Mitutoyo,Japan)对试样进行初始显微硬度测试。在所有样品的中心放置两个彼此相距100mm的压痕。使用激光和不使用激光使用过氧化氢进行漂白,然后在组(组1a和2a)中再矿化,然后进行侵蚀。使用上述用于初始显微硬度的方法测量最终硬度。结果:再矿化组的显微硬度变化最小。与未激光漂白的再矿化组相比,激光漂白与再矿化一起进行的组显示出显微硬度的改善,但数据没有统计学意义。与未进行再矿化的组相比,激光和再矿化组之间存在显著差异。结论:漂白后的牙釉质表面更易发生侵蚀,应采用再矿化剂提高漂白后的釉质显微硬度。二极管激光漂白和再矿化剂的组合提高了漂白搪瓷的显微硬度,从而证明了这种组合是有效的。
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引用次数: 0
Effect of ethylenediaminetetraacetic acid, maleic acid, and fumaric acid on postendodontic treatment root fracture toughness - An in vitro study. 乙二胺四乙酸、马来酸和富马酸对根管治疗后根断裂韧性的影响——体外研究。
Q2 Dentistry Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/jcd.jcd_252_23
Aabha Gautam Dave, P Laxmish Mallya, N Vasudev Ballal, Ramya Shenoy

Aim: The aim of this study was to evaluate the effectiveness of ultrasonic activation of ethylenediaminetetraacetic acid (EDTA), maleic acid (MA), and fumaric acid (FA) in combination with sodium hypochlorite (NaOCl) on postendodontic treatment root fracture toughness.

Materials and methods: Forty single-rooted mandibular premolars were sectioned below the cementoenamel junction and were kept on average 14 mm long. With the exception of eight randomly selected teeth acting as a negative control group (Group I/nonprepared), the rest were prepared till F3. Thirty-two specimens were randomly placed into four groups equally and then irrigated using the final irrigation protocols. Saline and NaOCl were used to irrigate Group II, 17% EDTA and NaOCl were used to irrigate Group III, 7% MA and NaOCl were used to irrigate Group IV, and 0.7% FA and NaOCl were used to irrigate Group V. The irrigants were ultrasonically activated in every group. The root canals were obturated with gutta-percha and AH Plus sealer. The roots were embedded along the long axis in the self-curing acrylic blocks along with polyvinyl siloxane, leaving the coronal 2 mm of the root exposed. The specimens were tested using a universal testing machine.

Statistical analysis: The Post hoc Tamhane's and one-way ANOVA tests were applied to examine the data.

Results: The result of the study revealed that 0.7% FA (598.09 N) provided better root fracture resistance than EDTA (428.07 N) and MA (511.87 N).

Conclusion: The conclusion derived from the study was that 0.7% FA provided better fracture resistance of endodontically treated teeth when compared to 17% EDTA and 7% MA.

目的:本研究的目的是评估乙二胺四乙酸(EDTA)、马来酸(MA)和富马酸(FA)与次氯酸钠(NaOCl)联合超声活化对根管治疗后根断裂韧性的有效性。材料和方法:将40颗单根下颌前磨牙在牙骨质-釉层交界处下方切开,平均保持14mm长。除了随机选择的8颗牙齿作为阴性对照组(第I组/非预备组)外,其余牙齿都预备到F3。32个样本被随机分为四组,每组平均,然后使用最终的灌溉方案进行灌溉。第二组用生理盐水和NaOCl灌溉,第三组用17%EDTA和NaOCl灌溉,第四组用7%MA和NaOCI灌溉,第五组用0.7%FA和NaOC1灌溉。根管用牙胶和AH-Plus封闭剂封闭。根沿着长轴与聚乙烯基硅氧烷一起嵌入自固化丙烯酸嵌段中,使根的冠部暴露2mm。使用通用试验机对试样进行试验。统计分析:采用Post-hoc Tamhane和单因素方差分析检验数据。结果:0.7%FA(598.09 N)比EDTA(428.07 N)和MA(511.87 N)具有更好的根断裂阻力。
{"title":"Effect of ethylenediaminetetraacetic acid, maleic acid, and fumaric acid on postendodontic treatment root fracture toughness - An <i>in vitro</i> study.","authors":"Aabha Gautam Dave, P Laxmish Mallya, N Vasudev Ballal, Ramya Shenoy","doi":"10.4103/jcd.jcd_252_23","DOIUrl":"10.4103/jcd.jcd_252_23","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to evaluate the effectiveness of ultrasonic activation of ethylenediaminetetraacetic acid (EDTA), maleic acid (MA), and fumaric acid (FA) in combination with sodium hypochlorite (NaOCl) on postendodontic treatment root fracture toughness.</p><p><strong>Materials and methods: </strong>Forty single-rooted mandibular premolars were sectioned below the cementoenamel junction and were kept on average 14 mm long. With the exception of eight randomly selected teeth acting as a negative control group (Group I/nonprepared), the rest were prepared till F3. Thirty-two specimens were randomly placed into four groups equally and then irrigated using the final irrigation protocols. Saline and NaOCl were used to irrigate Group II, 17% EDTA and NaOCl were used to irrigate Group III, 7% MA and NaOCl were used to irrigate Group IV, and 0.7% FA and NaOCl were used to irrigate Group V. The irrigants were ultrasonically activated in every group. The root canals were obturated with gutta-percha and AH Plus sealer. The roots were embedded along the long axis in the self-curing acrylic blocks along with polyvinyl siloxane, leaving the coronal 2 mm of the root exposed. The specimens were tested using a universal testing machine.</p><p><strong>Statistical analysis: </strong>The <i>Post hoc</i> Tamhane's and one-way ANOVA tests were applied to examine the data.</p><p><strong>Results: </strong>The result of the study revealed that 0.7% FA (598.09 N) provided better root fracture resistance than EDTA (428.07 N) and MA (511.87 N).</p><p><strong>Conclusion: </strong>The conclusion derived from the study was that 0.7% FA provided better fracture resistance of endodontically treated teeth when compared to 17% EDTA and 7% MA.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"453-457"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10315947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Does the presence of full-coverage crown interfere with removal of obturation material: A cone-beam computed tomography image segmentation volumetric analysis. 全覆盖牙冠的存在是否会干扰堵塞材料的去除:锥形束计算机断层扫描图像分割体积分析。
Q2 Dentistry Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/jcd.jcd_300_23
Abhishek Bhargava, Vineeta Nikhil

Aim: The aim of this study was to assess and compare the influence of the presence of full-coverage crown on the obturation material removal efficiency of different retreatment file systems.

Materials and methods: Sixty extracted, human, maxillary premolars were accessed, instrumented, and obturated with gutta-percha and AH Plus sealer. The samples were scanned with cone-beam computed tomography and volume of obturating material was measured using ITK-SNAP volumetric software. Samples were divided (n = 30) into with and without all metal full-coverage crown groups. Each group was then subdivided into three subgroups (n = 10) based on retreatment file used (ProTaper URS, HyFlex Remover, and NeoEndo). The volume of remnants of obturating material was remeasured by image segmentation using ITK-SNAP volumetric software. One-way analysis of variance, followed by the Tukey's post hoc test, was used for data analysis, with a significance level of 5%.

Results: A significant difference in percentage remnant of obturating material after retreatment file was observed on overall comparison between with (29.84%) and without crown (21.79%) groups (P < 0.05). Each file system removed significantly more obturating material in without crown samples than with crown samples (P < 0.05). The percentage remnant of obturating material after retreatment file use was as follows: ProTaper URS > NeoEndo retreatment file > HyFlex Remover. All file systems showed significantly different (P < 0.05) efficiency in removal of obturating material.

Conclusions: None of the retreatment file systems were able to remove obturating material completely. The presence of full-coverage crown negatively impacted the efficiency of retreatment files for the removal of obturating material. HyFlex Remover performed better than NeoEndo which was better than ProTaper URS.

目的:本研究的目的是评估和比较全覆盖牙冠的存在对不同再处理锉刀系统的堵塞材料去除效率的影响。材料和方法:取下60颗人类上颌前磨牙,用牙胶和AH-Plus封闭剂固定。使用锥形束计算机断层扫描对样品进行扫描,并使用ITK-SNAP体积软件测量充填材料的体积。将样品(n=30)分为具有和不具有全金属全覆盖冠的组。然后,根据使用的再治疗文件(ProTaper URS、HyFlex Remover和NeoEndo),将每组分为三个亚组(n=10)。使用ITK-SNAP体积软件通过图像分割重新测量充填材料的残余体积。数据分析采用单向方差分析,然后采用Tukey的事后检验,结果:与无冠组(21.79%)和有冠组(29.84%)相比,再处理锉刀后充填材料残留率有显著性差异(P<0.05),无冠组各锉刀系统去除的充填材料明显多于有冠组的充填材料(P<0.05)再处理锉使用后的材料如下:ProTaper URS>NeoEndo再处理锉>HyFlex Remover。所有文件系统在去除堵塞材料方面表现出显著差异(P<0.05)。结论:没有一个再治疗文件系统能够完全去除堵塞材料。全覆盖牙冠的存在对去除堵塞材料的再处理锉的效率产生了负面影响。HyFlex Remover的表现优于NeoEndo,后者优于ProTaper URS。
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引用次数: 0
Comparative evaluation of the quality and homogeneity of different obturating systems using cone-beam computed tomography - An in vitro study. 使用锥形束计算机断层扫描对不同密闭系统的质量和均匀性进行比较评估——一项体外研究。
Q2 Dentistry Pub Date : 2023-07-01 Epub Date: 2023-07-28 DOI: 10.4103/jcd.jcd_229_23
Grusha Gupta, Munish Singla, Harleen Kaur, Litik Mittal, Saloni Gupta, Savrose Kaur

Aim: The aim of this study was to evaluate the homogeneity and quality of different obturating systems using cone-beam computed tomography (CBCT).

Materials and methods: One hundred and twenty extracted maxillary and mandibular molars were included in this study which was decoronated first and then dissected into palatal and distal roots. After working length determination, all the roots were biomechanically prepared with ProTaper universal system. The prepared roots were evaluated using CBCT, and volumetric analysis was done. Samples were then randomly divided into three groups corresponding to the obturation method used - cold lateral compaction technique, single-cone technique, and BeeFill 2 in 1 system. After obturation, CBCT imaging and volumetric analyses were performed again to compare and analyze the amount of volume of obturation in different obturating techniques. To standardize the volume, pre-and postobturation images were superimposed. To avoid errors, for each section, measurements were repeated three times and the means were calculated.

Statistical analysis used: The data were statistically analyzed using one-way analysis of variance, followed by post hoc test.

Results: Results showed a statistically significant difference of the single cone with lateral compaction and the BeeFill system (P < 0.001). Maximum volume of obturating material was found in BeeFill at all levels - Coronal, middle, and apical, followed by cold lateral compaction and single-cone technique (P < 0.001).

Conclusion: It could be concluded that the thermoplasticized obturating technique can be a better choice for obturation rather than the single cone and lateral compaction technique.

目的:本研究的目的是使用锥形束计算机断层扫描(CBCT)评估不同封闭系统的均匀性和质量。材料和方法:本研究包括120颗拔除的上颌和下颌磨牙,这些磨牙先去角质,然后解剖成腭根和远根。在确定工作长度后,使用ProTaper通用系统对所有根进行生物力学制备。使用CBCT对制备的根进行评估,并进行体积分析。然后将样品随机分为三组,对应于所使用的封闭方法——冷侧向压实技术、单锥技术和BeeFill二合一系统。闭孔后,再次进行CBCT成像和体积分析,以比较和分析不同闭孔技术的闭孔体积。为了使体积标准化,将术前和术后图像叠加。为了避免误差,对每个截面重复测量三次,并计算平均值。使用的统计分析:使用单向方差分析对数据进行统计分析,然后进行事后检验。结果:结果显示,横向压实的单锥和BeeFill系统在统计学上有显著差异(P<0.001)。BeeFill在所有级别(冠状、中间和顶端)都发现了最大体积的充填材料,结论:热塑性充填技术是一种比单锥侧压充填技术更好的充填方法。
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引用次数: 0
Inhibitory effect of nano Stolephorus insularis and calcium hydroxide on glucosyltransferase (GTF) activity of Lactobacillus aciophilus 纳米竹霉和氢氧化钙对嗜酸乳杆菌葡萄糖基转移酶活性的抑制作用
Q2 Dentistry Pub Date : 2023-06-30 DOI: 10.20473/cdj.v13i1.2023.29-32
Tamara Yuanita, D. E. Juniarti, Tarsardo Marbun
Background: Dental caries is the most common multifactorial infectious disease worldwide which referes to the process of destruction tooth hard tissue caused by bacterial by-products in the form of acids through carbohydrate fermentation. Lactobacillus acidophilus is one of the main cariogenic bacteria that causes caries by forming biofilms with the help ofglucosyltransferase (GTF) enzymes. Calcium hydroxide has limited antibacterial effects. Nano brown anchovy contains fluor as the active compound which has the potential as an antibacterial alternative agent. Purpose: To explain the inhibition of the combination of nano Stolephorus insularis and calcium hydroxide on the activity of the glucosyltransferase enzyme of Lactobacillus acidophilus bacteria. Methods: This study used a combination of nano Stolephorus insularis 3.125% with calcium hydroxide as a treatment and aquades as a control. The GTF enzyme was obtained from the supernatant centrifuged by Lactobacillus acidophilus in BHIB. The activity of the GTF enzyme was considered by calculating the fructose levels using High Performance Liquid Chromatography (HPLC) in a certain formula.Results: Decreased levels of fructose was obtained in the treatment group. From the results of Mann- Whitney data analysis, there were significant difference in the study groups. Conclusion: the combination of nano Stolephorus insularis with calcium hydroxide is effective to inhibit the glucosyltransferase enzyme activity of Lactobacillus acidophilus bacteria.
背景:龋齿是世界范围内最常见的多因素感染性疾病,是指细菌副产物通过碳水化合物发酵以酸的形式破坏牙齿硬组织的过程。嗜酸乳杆菌是主要的致龋细菌之一,它在葡萄糖基转移酶(GTF)酶的帮助下形成生物膜,从而导致龋齿。氢氧化钙的抗菌作用有限。纳米褐凤尾鱼含有氟作为活性化合物,有潜力成为一种抗菌替代剂。目的:探讨纳米岛栗与氢氧化钙联合使用对嗜酸乳杆菌葡萄糖基转移酶活性的抑制作用。方法:采用纳米岛栗3.125%与氢氧化钙联合处理,水合液作为对照。从嗜酸乳杆菌在BHIB中离心的上清液中获得GTF酶。采用高效液相色谱法(HPLC)计算一定公式中的果糖含量,考虑GTF酶的活性。结果:治疗组果糖含量明显降低。从Mann- Whitney资料分析结果来看,各研究组间存在显著差异。结论:纳米竹霉与氢氧化钙联合使用可有效抑制嗜酸乳杆菌葡萄糖基转移酶活性。
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引用次数: 0
The effect addition of epigallocatechin-3-gallate (EGCG) in nano hydroxyapatite on surface porosity as a candidate pulp capping materia 表没食子儿茶素-没食子酸酯(EGCG)作为纳米羟基磷灰石的复盖材料,对其表面孔隙率的影响
Q2 Dentistry Pub Date : 2023-06-30 DOI: 10.20473/cdj.v13i1.2023.24-28
Kun Ismiyatin, N. Zubaidah, Indira Moza Azzaria
Background: Deep caries, cavity preparation and use of burs or other dental instruments often result in pulpal perforation. In the case of an exposed pulp, regenerative pulp tissue treatment aims to regenerate normal tissue and maintain pulp vitality by inducing pulp regeneration using the right material, so that a good percentage and size of material porosity is needed to help cell regeneration by supporting cell proliferation and attachment, stimulating remineralization and differentiation of odontoblast-like cells. Nano-HA has the ability to produce dentine bridges continuously, has porous properties that allow cell growth, improves mechanical properties, but is not anti-inflammatory so that EGCG is added which has the advantage of being an antioxidant, anti-inflammatory which can optimize pulp tissue regeneration and also acts as an antimicrobial by reduce the growth of bacteria in the oral cavity and can trigger the proliferation and differentiation of human dental pulp cells. Purpose: This study aims to prove the difference in surface porosity of nano hydroxyapatite added with EGCG compared to nano hydroxyapatite and aquadest. Methods: This study used a laboratory experimental study with a posttest-only control group design. The research sample consisted of 32 samples which were divided into 2 groups, the treatment group (nano HA - EGCG) and the control group (nano HA - aquadest). Each research group was subjected to freeze drying and SEM tests. Results: There was a significant difference in the percentage of surface porosity between the nano hydroxyapatite added with EGCG compared to nano hydroxyapatite and aquadest.on the results of the Independent T-test (p<0.05). Conclusion: The addition of EGCG to nano hydroxyapatite has a higher porosity percentage compared to nano hydroxyapatite with aquadest.
背景:深龋、空腔准备和使用牙刺或其他牙科器械常导致牙髓穿孔。在牙髓暴露的情况下,再生牙髓组织治疗的目的是通过使用合适的材料诱导牙髓再生来再生正常组织和维持牙髓活力,因此需要一个良好的材料孔隙率和大小,通过支持细胞增殖和附着,刺激成牙髓样细胞的再矿化和分化来帮助细胞再生。纳米透明质酸具有持续产生牙本质桥的能力,具有允许细胞生长的多孔特性,改善机械性能,但不具有抗炎作用,因此添加EGCG具有抗氧化剂的优点,抗炎作用可以优化牙髓组织再生,还可以通过减少口腔内细菌的生长来起到抗菌作用,并可以触发人类牙髓细胞的增殖和分化。目的:本研究旨在证明添加EGCG的纳米羟基磷灰石表面孔隙率与纳米羟基磷灰石和水石的差异。方法:本研究采用实验室实验研究,采用纯后测对照组设计。研究样本共32份,分为2组,处理组(纳米HA - EGCG)和对照组(纳米HA - aquadest)。每个研究组进行冷冻干燥和扫描电镜测试。结果:添加EGCG的纳米羟基磷灰石的表面孔隙率与纳米羟基磷灰石和水石相比有显著差异。对独立t检验结果的影响(p<0.05)。结论:EGCG添加到纳米羟基磷灰石中比添加水基石的纳米羟基磷灰石具有更高的孔隙率。
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引用次数: 0
Toxicity test of bioceramic biphasic calcium phospate (BCP) Sr-Ag doping as bone graft in BHK-21 fibroblast cells 生物陶瓷双相磷酸钙(BCP) Sr-Ag掺杂骨移植对BHK-21成纤维细胞的毒性试验
Q2 Dentistry Pub Date : 2023-06-30 DOI: 10.20473/cdj.v13i1.2023.33-36
D. Wahjuningrum, S. Setyabudi, Ria Chusnita
Background: Bone graft is a substitute material that is used to assist reconstruction, stabilize the structure and bonds in bone, stimulate the osteogenesis process and as a healing bone defect. One type of bone graft that has good osteoinductive and bicompatibility is alloplast which is a synthetic calcium phosphate compound. The most frequently used Calcium Phosphate groups are Hydroxyapatite (HA), β-Tricalcium Phosphate (β-TCP), and Biphasic Calcium Phosphate (BCP). In this study the material used was BCP doping Sr2+ and Ag+. Strontium ions (Sr2+) can increase osteoblast activity, reduce osteoclast activity and cytokine production, improve osteointegration, and minimize fractures. Ag+ ion has the ability as an antibacterial agent. Purpose: To explain and prove the toxicity of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as bone graft on BHK-21 fibroblast cells. Methods: This type of research is a laboratory experiment with a post-test only control group design. Treatment with Biphasic Calcium Phosphate (BCP) doped Sr-Ag with concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm in BHK-21 fibroblast cell culture. Results: The percentage of fibroblast cell life at concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm, respectively, the percentage of live cells was 38% 44%, 46%, 50%, 52%, 65%, 69%, 71%, 72%, 75%, 77%, 81%, and 87%. The parameter used in this toxicity test is CD50. Conclusion: The results of the toxicity test of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as a bone graft showed a toxic and non-toxic effect on BHK-21 fibroblast cells at certain concentrations.
背景:骨移植物是一种替代材料,用于辅助重建,稳定骨结构和骨结合,刺激成骨过程和愈合骨缺损。异形体是一种合成磷酸钙化合物,是一种具有良好骨诱导和双相容性的骨移植物。最常用的磷酸钙基团是羟基磷灰石(HA)、β-磷酸三钙(β-TCP)和双相磷酸钙(BCP)。本研究使用的材料是BCP掺杂Sr2+和Ag+。锶离子(Sr2+)可以增加成骨细胞活性,降低破骨细胞活性和细胞因子的产生,改善骨整合,减少骨折。银离子具有抗菌剂的作用。目的:解释和证明生物陶瓷双相磷酸钙(BCP)掺杂Sr-Ag作为骨移植物对BHK-21成纤维细胞的毒性。方法:本研究为实验室实验,仅采用后测对照组设计。在BHK-21成纤维细胞培养中,用双相磷酸钙(BCP)掺杂浓度为200 ppm、180 ppm、160 ppm、140 ppm、120 ppm、80 ppm、40 ppm、20 ppm、10 ppm、5 ppm、2.5 ppm、1.25 ppm、0.625 ppm的Sr-Ag处理。结果:在200 ppm、180 ppm、160 ppm、140 ppm、120 ppm、80 ppm、40 ppm、20 ppm、10 ppm、5 ppm、2.5 ppm、1.25 ppm、0.625 ppm的浓度下,成纤维细胞的活细胞率分别为38%、44%、46%、50%、52%、65%、69%、71%、72%、75%、77%、81%、87%。本毒性试验的参数为CD50。结论:生物陶瓷双相磷酸钙(Biphasic Calcium Phosphate, BCP)掺杂Sr-Ag作为骨移植物,在一定浓度下对BHK-21成纤维细胞有毒性和无毒性作用。
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引用次数: 0
期刊
Journal of Conservative Dentistry
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