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Effect of VP, MAP and combined packaging systems on the physicochemical properties and microbiological status of veal from unweaned calves VP、MAP 和组合包装系统对未断奶小牛小牛肉理化特性和微生物状态的影响。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-07-03 DOI: 10.1016/j.meatsci.2024.109590
Marek Kowalczyk , Piotr Domaradzki , Monika Ziomek , Piotr Skałecki , Agnieszka Kaliniak-Dziura , Paweł Żółkiewski , Anna Chmielowiec-Korzeniowska , Monika Kędzierska-Matysek , Aleksandra Ukalska-Jaruga , Tomasz Grenda , Roberta Nuvoloni , Mariusz Florek

The packaging system is one of the factors influencing the preservation of the nutritional value, microbiological safety, and sensory attributes of meat. The study investigated changes in physicochemical and microbiological properties taking place during 15-day refrigerated storage of two calf muscles, the longissimus lumborum (LL) and semitendinosus (ST), packaged in three systems, respectively, vacuum packing (VP), modified atmosphere packaging (MAP, 80% O2 + 20% CO2), and a combined system (VP + MAP, 8 d in VP followed by 7 d in MAP). LL and ST stored in VP had significantly lower levels of lipid oxidation, higher α-tocopherol content, and higher instrumentally measured tenderness in comparison with the samples stored in MAP. On the other hand, the MAP samples had lower purge loss at 5 and 15 days, a higher proportion of oxymyoglobin up to 10 days of storage, and a better microbiological status. Calf muscle samples stored in the VP + MAP system had intermediate values for TBARS and α-tocopherol content and at the same time were the most tender and had the lowest counts of Pseudomonas and Enterobacteriaceae bacteria at 15 days. All packaging systems ensured relatively good quality of veal characteristics up to the last day of storage. However, for MAP at 15 days of storage, unfavourable changes in colour (a high level of metmyoglobin and a decrease in oxymyoglobin, redness and R630/580 ratio) and in the lipid fraction (a high TBARS value and a significant decrease in α-tocopherol content) were observed.

包装系统是影响肉类营养价值、微生物安全和感官特性保存的因素之一。该研究调查了两种小牛肌肉(腰长肌(LL)和半腱肌(ST))在 15 天冷藏贮存期间理化和微生物特性的变化,这两种肌肉分别在真空包装(VP)、改良气氛包装(MAP,80% O2 + 20% CO2)和组合包装(VP + MAP,在 VP 中贮存 8 天,然后在 MAP 中贮存 7 天)这三种系统中进行包装。与在 MAP 中储存的样品相比,在 VP 中储存的 LL 和 ST 的脂质氧化水平明显较低,α-生育酚含量较高,仪器测量的嫩度也较高。另一方面,MAP 样品在 5 天和 15 天时的净化损失较低,储存 10 天时氧肌红蛋白的比例较高,微生物状况较好。在 VP + MAP 系统中储存的小牛肌肉样品的 TBARS 和 α-生育酚含量处于中间值,同时在 15 天时肉质最嫩,假单胞菌和肠杆菌科细菌的数量最少。所有的包装系统都能确保小牛肉在最后一天的贮藏过程中保持相对较好的品质。不过,对于贮藏 15 天的 MAP 来说,色泽(高水平的偏肌红蛋白和氧肌红蛋白、红度和 R630/580 比率下降)和脂质部分(高 TBARS 值和α-生育酚含量显著下降)都出现了不利的变化。
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引用次数: 0
Marine macroalgae in rabbit feed – Effects on meat quality 兔饲料中的海洋大型藻类 - 对肉质的影响
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-07-03 DOI: 10.1016/j.meatsci.2024.109584
Sabela Al-Soufi , Javier García , Nuria Nicodemus , Jose M. Lorenzo , Eugenio Cegarra , Antonio Muíños , Ana Paula Losada , Marta Miranda , Marta López-Alonso

The addition of macroalgae to livestock diets has demonstrated to enhance the quality of meat by improving the muscle stability, antioxidant capacity and fatty acid profile. However, information regarding rabbit meat is scarce. This study evaluated the effect of adding 1.025% of different macroalgae, dehydrated and as extracts (Saccharina latissima, Himanthalia elongata and Ulva spp.) to the diet of growing rabbits. Dietary supplementation with the Ulva spp. extract increased the fat content (0.96% vs 0.33% in control group) and the proportion of monounsaturated fatty acids (by 22%; P ≤ 0.022), but did not affect the moisture, protein or ash contents or the physicochemical properties of the rabbit longissiumus lumborum muscle. The antioxidant status of the meat was adequate and was not affected by the dietary supplements. The sensorial properties of the meat were also not affected, and dietary supplementation with both S. latissima and H. elongata actually enhanced the flavour and juiciness of the meat (P ≤ 0.01). Altogether, the study findings indicate that the addition of these sustainable ingredients to rabbit feed did not negatively affect meat quality, and some of them may potentially improve specific characteristics, which could make this meat more attractive to consumers.

事实证明,在牲畜日粮中添加大型藻类可提高肌肉稳定性、抗氧化能力和脂肪酸含量,从而提高肉质。然而,有关兔肉的信息却很少。本研究评估了在生长期兔子的日粮中添加 1.025%的不同大型藻类(Saccharina latissima、Himanthalia elongata 和 Ulva spp.)脱水提取物的效果。膳食中补充莼菜提取物可增加脂肪含量(0.96% 对对照组的 0.33%)和单不饱和脂肪酸比例(22%;P ≤ 0.022),但不会影响水分、蛋白质、灰分含量或兔腰长肌的理化性质。肉的抗氧化状态良好,不受膳食补充剂的影响。肉的感官特性也没有受到影响,而且膳食补充剂 S. latissima 和 H. elongata 实际上增强了肉的风味和多汁性(P ≤ 0.01)。总之,研究结果表明,在兔饲料中添加这些可持续成分不会对肉的质量产生负面影响,其中一些成分还可能改善肉的特定特征,从而使这种肉对消费者更具吸引力。
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引用次数: 0
Replacing the animal fat in Bologna sausages using high internal phase emulsion stabilized with lentil protein isolate (Lens culinaris) 用小扁豆分离蛋白(Lens culinaris)稳定的高内相乳液替代博洛尼亚香肠中的动物脂肪
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-07-02 DOI: 10.1016/j.meatsci.2024.109589
Andrêssa Maria Medeiros Theóphilo Galvão, Gilmar Freire da Costa, Mirian dos Santos, Marise Aparecida Rodrigues Pollonio, Míriam Dupas Hubinger

High internal phase emulsions (HIPEs) are promising techniques that can replace saturated fat in food without reducing the product's texture, sensory attributes, water-holding capacity, and cooking loss. In the current investigation, 100% pork back fat was replaced by HIPEs formed with lentil protein isolate (LPI) in Bologna sausages. HIPEs were prepared by 25% LPI dispersion (2, 4, 6, and 8%, w/w) and 75% (w/w) soybean oil. HIPEs with higher LPI concentration (4, 6, and 8%, w/w) showed lower droplet size, firmer appearance, and better rheology behavior than 2% LPI. The concentrations LPI (2%, 4%, 6%, and 8%, w/w) led to increased moisture in sausages (FH2, FH4, FH6, and FH8, respectively) compared to the FC. These LPI levels resulted in sausage values for pressed juice similar to the FC and lower energy values than sausages with soybean oil (FO) and pork back fat (FC). Besides, these LPI concentrations (4%, 6%, and 8%, w/w) resulted in a lower oil oxidation level in sausages with HIPEs (FH4, FH6, and FH8, respectively) compared to the control sausage formulation with pork back fat (FC). Bologna sausages elaborated with HIPEs showed emulsion stability values higher than 97%, without significance difference between them. The texture and sensory properties of sausages made with HIPEs were comparable to those made with pork back fat. HIPEs may improve the oxidation stability of the Bologna sausages. These results highlight the effectiveness of HIPEs structured with lentil protein in successfully substituting pork back fat in Bologna sausages with a better nutritional appeal.

高内相乳液(HIPE)是一种很有前景的技术,它可以替代食品中的饱和脂肪,同时不会降低产品的质地、感官特性、持水能力和烹饪损耗。在目前的研究中,博洛尼亚香肠中的 100%猪背部脂肪被小扁豆蛋白分离物(LPI)形成的 HIPEs 所替代。HIPE 由 25% 的 LPI 分散液(2%、4%、6% 和 8% w/w)和 75% (w/w)的大豆油制备而成。与 2% 的 LPI 相比,LPI 浓度较高(4%、6% 和 8% w/w)的 HIPE 显示出较小的液滴尺寸、更坚实的外观和更好的流变性能。与 FC 相比,LPI 浓度(2%、4%、6% 和 8% w/w)会导致香肠(分别为 FH2、FH4、FH6 和 FH8)的水分增加。与添加大豆油(FO)和猪背脂肪(FC)的香肠相比,这些 LPI 水平导致压榨果汁的香肠值与 FC 相似,而能量值较低。此外,与使用猪背脂肪(FC)的对照香肠配方相比,这些 LPI 浓度(4%、6% 和 8% w/w)导致使用 HIPE(分别为 FH4、FH6 和 FH8)的香肠中油的氧化水平较低。使用 HIPE 制作的博洛尼亚香肠的乳化稳定性高于 97%,但两者之间没有显著差异。用 HIPE 制作的香肠的质地和感官特性与用猪背脂肪制作的香肠相当。HIPEs 可以提高博洛尼亚香肠的氧化稳定性。这些结果凸显了以扁豆蛋白为结构的 HIPE 在成功替代猪背脂肪制作博洛尼亚香肠方面的有效性,并具有更好的营养吸引力。
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引用次数: 0
Understanding Consumer Preferences for Attributes of Yak Meat: Implications for Economic Growth and Resource Efficiency in Pastoral Areas 了解消费者对牦牛肉属性的偏好:对牧区经济增长和资源效率的影响。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-07-02 DOI: 10.1016/j.meatsci.2024.109586
Yongshun Ai , Rao Yuan , Shaosheng Jin , Wen Lin , Yan Zhang

Realizing the market value of grassland resources is of great significance to finding a balance between ecological protection and economic development. As a unique livestock animal that is native to the Qinghai-Xizang Plateau, the yak plays an important role in maintaining ecosystem stability, ensuring the livelihoods of herdsmen, promoting socio-economic development, and preserving ethnic cultural traditions. However, given its small market share, little is known about the factors that drive Chinese consumer preferences for yak meat. This study aimed to investigate consumer preferences for yak meat by means of an online survey involving a sample of 2999 respondents from five cities in China. The best-worst scaling method was employed to measure the relative importance of different attributes of yak meat by using a purchasing scenario. The results indicated that quality-safety certification, freshness, and production sustainability were regarded as the most preferred attributes, whereas animal welfare, brand, and the purchasing channel were found to be the least preferred. In addition, significant heterogeneity was detected in consumer preferences. Consumers were divided into three classes, namely ‘Price Sensitive Consumers,’ ‘Environmentally Friendly Consumers,’ and ‘Yak Meat Inclined Consumers,’ respectively. Our findings might be helpful for policymakers and yak meat producers to develop targeted strategies to facilitate the sale of yak meat and then restore degraded grasslands.

实现草原资源的市场价值,对于寻求生态保护与经济发展之间的平衡具有重要意义。牦牛作为一种原产于青藏高原的独特牲畜,在维护生态系统稳定、保障牧民生计、促进社会经济发展、传承民族文化传统等方面发挥着重要作用。然而,由于牦牛肉的市场份额较小,中国消费者对牦牛肉偏好的驱动因素知之甚少。本研究旨在通过在线调查的方式,调查中国五个城市 2999 名受访者对牦牛肉的消费偏好。研究采用了最佳-最差比例法,通过购买情景来衡量牦牛肉不同属性的相对重要性。结果表明,质量安全认证、新鲜度和生产可持续性被认为是最受欢迎的属性,而动物福利、品牌和购买渠道则是最不受欢迎的属性。此外,在消费者偏好中还发现了明显的异质性。消费者被分为三类,分别是 "价格敏感型消费者"、"环境友好型消费者 "和 "牦牛肉偏好型消费者"。我们的研究结果可能有助于政策制定者和牦牛肉生产者制定有针对性的策略,促进牦牛肉的销售,进而恢复退化的草原。
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引用次数: 0
Evaluation of nutritional, technological, oxidative, and sensory properties of low-sodium and phosphate-free mortadellas produced with bamboo fiber, pea protein, and mushroom powder 评估用竹纤维、豌豆蛋白和蘑菇粉生产的低钠无磷酸盐砂糖的营养、技术、氧化和感官特性。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.1016/j.meatsci.2024.109588
Mariana Basso Pinton , José Manuel Lorenzo , Bibiana Alves dos Santos , Leticia Pereira Correa , Milena Padilha , Pamela Cristiele Oliveira Trindade , Alexandre José Cichoski , Roberto Bermúdez , Laura Purriños , Paulo Cezar Bastianello Campagnol

This study examined the effects of replacing alkaline phosphate (AP) with bamboo fiber (BF), isolated pea protein (PP), and mushroom powder (MP) on the nutritional, technological, oxidative, and sensory characteristics of low-sodium mortadellas. Results indicated that this reformulation maintained the nutritional quality of the products. Natural substitutes were more effective than AP in reducing water and fat exudation. This led to decreased texture profile analysis (TPA) values such as hardness, cohesiveness, gumminess, and chewiness. The reformulation reduced the L* values and increased the b* values, leading to color modifications rated from noticeable to appreciable according to the National Bureau of Standards (NBS) index. Despite minor changes in oxidative stability indicated by increased values in TBARS (from 0.19 to 0.33 mg MDA/kg), carbonyls (from 2.1 to 4.4 nmol carbonyl/mg protein), and the volatile compound profile, the sensory profile revealed a beneficial increase in salty taste, especially due to the inclusion of MP, which was enhanced by the synergy with BF and PP. In summary, the results confirmed the potential of natural alternatives to replace chemical additives in meat products. Incorporating natural antioxidants into future formulations could address the minor oxidation issues observed and enhance the applicability of this reformulation strategy.

本研究考察了用竹纤维(BF)、分离豌豆蛋白(PP)和蘑菇粉(MP)替代碱性磷酸(AP)对低钠钵仔糕的营养、工艺、氧化和感官特性的影响。结果表明,这种改良配方保持了产品的营养质量。在减少水分和脂肪渗出方面,天然替代品比 AP 更有效。这导致质构分析(TPA)值下降,如硬度、粘稠度、胶质感和咀嚼感。重新配方降低了 L* 值,提高了 b* 值,根据美国国家标准局(NBS)的指数,颜色变化从明显到显著不等。尽管 TBARS(从 0.19 到 0.33 毫克 MDA/公斤)、羰基(从 2.1 到 4.4 毫摩尔羰基/毫克蛋白质)和挥发性化合物含量的增加表明氧化稳定性发生了轻微变化,但感官特征显示咸味有了有益的增加,特别是由于添加了 MP,而 MP 与 BF 和 PP 的协同作用又增强了咸味。总之,研究结果证实了天然替代品取代肉制品中化学添加剂的潜力。在未来的配方中加入天然抗氧化剂可以解决观察到的轻微氧化问题,并提高这种改良配方策略的适用性。
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引用次数: 0
As a reviewer, how do I decide between recommending rejection of a paper rather than a major revision? 作为审稿人,我如何决定建议拒绝论文而不是进行重大修改?
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-29 DOI: 10.1016/j.meatsci.2024.109587
Peter P. Purslow

An associate editor of the journal has experienced an increase in the number of received reviews where the comments to either the authors or to the editor do not align with the reviewer's recommendation to accept, revise or reject. In particular, some recommendations for outright rejection of a submission have been accompanied by criticisms that clearly could have been solved by revision of the manuscript. The purpose of this letter is to provide some guidance to reviewers on the specific issue of deciding between a recommendation to revise versus reject.

该期刊的一位副主编发现,收到的审稿意见中,给作者或编辑的意见与审稿人提出的接受、修改或拒稿建议不一致的情况越来越多。特别是,有些建议直接拒绝投稿的同时,还提出了一些显然可以通过修改稿件来解决的批评意见。本信的目的是为审稿人提供一些指导,帮助他们在建议修改还是退稿的具体问题上做出决定。
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引用次数: 0
Improving the storage quality of Harbin red sausages by quaternized chitosan/sodium alginate coating curcumin nano-emulsion 季铵化壳聚糖/海藻酸钠包覆姜黄素纳米乳液改善哈尔滨红肠的贮藏品质
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-28 DOI: 10.1016/j.meatsci.2024.109585
Ying-ao Zhu, Fei Li, Meihui Wang, Yuhang Cao, Baohua Kong, Qian Liu, Hui Wang, Qian Chen

In this study, the effect of sodium alginate and quaternized chitosan bis-polysaccharide-based shell transport curcumin nano-emulsions (Cur@QCS/SA) on the microbiological, physicochemical properties, quality characteristics of Harbin red sausage during storage is investigated. According to the microbiological results, the shelf life of Harbin red sausage is extended from 3 d to 6 d by adding 0.15% Cur@QCS/SA, and Bacillus is the most predominant bacterial before 6 d. Additionally, the physicochemical properties change significantly, the pH, weight loss (WL), water holding capacity (WHC), water activity (aw), L*, and a* of red sausage decrease gradually with the extension of storage time, as well as b*, lipid oxidation, proteolysis increase significantly (P < 0.05). Secondly, it is found that 0.15% treatment group can better maintain the quality characteristics of Harbin red sausage according to texture profile analysis (TPA), electronic nose (E-nose), and electronic tongue (E-tongue) (P < 0.05). This study provides a new way for nano-emulsions in food applications and a new option for the preservation of Harbin red sausage as well as other low-temperature meat products.

本研究探讨了海藻酸钠和季铵化壳聚糖双多糖壳输送姜黄素纳米乳液(Cur@QCS/SA)对哈尔滨红肠贮藏过程中微生物、理化性质和品质特征的影响。微生物学结果表明,添加 0.15% Cur@QCS/SA 后,哈尔滨红肠的保质期由 3 d 延长至 6 d,6 d 前最主要的细菌是芽孢杆菌;理化性质发生了显著变化,红肠的 pH 值、失重(WL)、持水量(WHC)、水分活度(aw)、L*、a*随贮藏时间的延长而逐渐降低,b*、脂质氧化、蛋白水解显著增加(P<0.05)。
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引用次数: 0
Comparative transcriptome analysis of slow-twitch and fast-twitch muscles in Kazakh horses 哈萨克马慢肌和快肌转录组比较分析。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-26 DOI: 10.1016/j.meatsci.2024.109582
Jianwen Wang , Wanlu Ren , Zhiwen Sun , Zixiang Han , Yaqi Zeng , Jun Meng , Xinkui Yao

This study conducted a thorough analysis of the myofiber type composition in the extensor digitorum longus muscle (EDL) and soleus muscle (SOL) of Kazakh horses, across different genders (male and female). The results showed significant differences in myofiber type composition between EDL and SOL, with a higher proportion of Type I fibers in SOL muscles and a greater prevalence of Type II fibers in EDL muscles. Additionally, the myofiber diameter in Kazakh horses was relatively small, potentially related to the tenderness and edible quality of their muscles. Using high-throughput sequencing technology, we constructed 32 cDNA sequencing libraries and obtained high-quality read data. Gene expression analysis revealed 278 and 372 differentially expressed genes (DEGs) in EDL and SOL muscles, respectively, including genes related to muscle contraction, metabolism, and development. Intersection analysis of DEGs between genders showed that 60 DEGs were significantly different in both male and female horses. GO annotation and KEGG analysis further elucidated the roles of these DEGs in muscle structure, function, and cellular signaling. Protein-protein interaction (PPI) network analysis and identification of hub genes provided new insights into the molecular mechanisms underlying muscle growth and development. Finally, the reliability of the DEGs data was validated through quantitative real-time PCR (qRT-PCR). This study not only enhances our understanding of the biological characteristics of horse muscles but also provides potential molecular targets for improving horse muscle performance and health.

本研究对哈萨克马不同性别(雄性和雌性)的伸肌(EDL)和比目鱼肌(SOL)的肌纤维类型组成进行了深入分析。结果显示,EDL和SOL肌肉的肌纤维类型组成存在明显差异,SOL肌肉中I型纤维的比例更高,而EDL肌肉中II型纤维的比例更高。此外,哈萨克马的肌纤维直径相对较小,这可能与其肌肉的柔软度和食用品质有关。我们利用高通量测序技术构建了 32 个 cDNA 测序文库,并获得了高质量的读数数据。基因表达分析显示,EDL和SOL肌肉中分别有278和372个差异表达基因(DEGs),包括与肌肉收缩、新陈代谢和发育相关的基因。性别间 DEGs 的交叉分析表明,60 个 DEGs 在雄马和雌马中存在显著差异。GO注释和KEGG分析进一步阐明了这些DEGs在肌肉结构、功能和细胞信号传导中的作用。蛋白质-蛋白质相互作用(PPI)网络分析和枢纽基因的鉴定为了解肌肉生长和发育的分子机制提供了新的视角。最后,通过实时定量 PCR(qRT-PCR)验证了 DEGs 数据的可靠性。这项研究不仅加深了我们对马肌肉生物学特性的了解,还为改善马肌肉性能和健康提供了潜在的分子靶标。
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引用次数: 0
Transcriptome analysis reveals the effects of Schizochytrium sp. on the meat quality attributes of Tan lambs 转录组分析揭示了裂头蚴对谭氏羔羊肉质属性的影响。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-26 DOI: 10.1016/j.meatsci.2024.109583
Changpeng Gao, Hongrui Wen, Dongwen Dai, Qingmin Li, Yuxiang Zhou

Schizochytrium sp., a feed additive, positively affects the quality of animal meat. In this study, the molecular mechanisms through which dietary Schizochytrium sp. affects the meat quality characteristics of Tan lambs were investigated using transcriptomic techniques. The findings demonstrate that the lambs supplemented with Schizochytrium sp. had a larger loin eye area and a higher average daily gain and intramuscular fat content (P < 0.05). They also had lower drip loss (at 24 and 48 h) and shear force (P < 0.05). Further, 745 genes were differentially expressed between lambs supplemented with Schizochytrium and the control group. Moreover, KEGG pathway analysis showed that the ECM-receptor interaction pathway, which is related to muscle generation and intramuscular fat deposition, was significantly enriched in the lambs administered a diet containing Schizochytrium sp. Herein, we identified some pivotal genes linked to muscular system development and lipid metabolism. Thus, using Schizochytrium sp. may boost the meat quality of Tan lambs by modifying the expression of genes related to hub pathways. The results supply a new basis to determine the molecular mechanisms through which Schizochytrium sp. supplementation regulates the meat quality characteristics of sheep.

Schizochytrium sp.是一种饲料添加剂,对动物肉质有积极影响。本研究利用转录组学技术研究了日粮中的Schizochytrium sp.影响谭氏羔羊肉质特征的分子机制。研究结果表明,添加五裂叶黑藻的羔羊腰眼面积更大,平均日增重和肌肉脂肪含量更高(P
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引用次数: 0
Effects of dietary arginine supplementation on muscle structure, meat characteristics and lipid oxidation products in lambs and its potential mechanisms of action 日粮中补充精氨酸对羔羊肌肉结构、肉质特征和脂质氧化产物的影响及其潜在作用机制
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-26 DOI: 10.1016/j.meatsci.2024.109581
Lu Dou , Chang Liu , Rina Su , Mirco Corazzin , Zhimin Jin , Zhihao Yang , Guanhua Hu , Min Zhang , Lina Sun , Lihua Zhao , Ye Jin , Lin Su

This study aimed to assess the effect of dietary arginine supplementation on muscle structure and meat characteristics of lambs also considering lipid oxidation products and to contribute to reveal its mechanisms of action using tandem mass tagging (TMT) proteomics. Eighteen lambs were allocated to two dietary treatment groups: control diet or control diet with the addition of 1% L-arginine. The results revealed that dietary arginine supplementation increased muscle fibre diameter and cross-sectional area (P < 0.05), which was attributable to protein deposition, as evidenced by increased RNA content, RNA/DNA ratio, inhibition of apoptotic enzyme activity, and alterations in the IGF-1/Akt signaling pathway (P < 0.05). In addition, dietary arginine elevated pH24h, a* values, and IMF content, decreased shear force value and backfat thickness (P < 0.05), as well as decreased the formation of lipid oxidation products involved in meat flavor including hexanal, heptanal, octanal, nonanal and 1-octen-3-ol by increasing the antioxidant capacity of the muscle (P < 0.05). The proteomics results suggested that seven enrichment pathways may be potential mechanisms by which arginine affected the muscle structure and meat characteristics of lambs. In summary, arginine supplementation in lamb diets provides a safe and effective way to improve meat quality, and antioxidant capacity of muscle of lamb.

本研究旨在评估日粮中补充精氨酸对羔羊肌肉结构和肉质特征的影响,同时考虑脂质氧化产物,并利用串联质量标记(TMT)蛋白质组学揭示其作用机制。18 只羔羊被分配到两个日粮处理组:对照日粮或添加 1%L-精氨酸的对照日粮。结果显示,日粮中补充精氨酸可增加肌肉纤维直径和横截面积(P < 0.05),这可归因于蛋白质沉积,RNA 含量、RNA/DNA 比值的增加、凋亡酶活性的抑制以及 IGF-1/Akt 信号通路的改变都证明了这一点(P < 0.05)。此外,日粮精氨酸可提高 pH24h 值、a* 值和 IMF 含量,降低剪切力值和背膘厚度(P <;0.05),并通过提高肌肉的抗氧化能力来减少肉味中脂质氧化产物的形成,包括己醛、庚醛、辛醛、壬醛和 1-辛烯-3-醇(P <;0.05)。蛋白质组学研究结果表明,精氨酸影响羔羊肌肉结构和肉质特征的潜在机制可能是七种富集途径。总之,在羔羊日粮中添加精氨酸可安全有效地改善羔羊肌肉的肉质和抗氧化能力。
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