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Dry-ageing impacts on meat quality, oxidative stability, and release of free amino acids in striploins from dairy crossbred yearling and 2-year-old steers 干龄对一岁和两岁奶牛杂交阉牛的肉质、氧化稳定性和游离氨基酸释放的影响
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-22 DOI: 10.1016/j.meatsci.2024.109674
Renyu Zhang, Noby Jacob, Michael Agnew, Mustafa M. Farouk, Carolina E. Realini
This study determined the impacts of dry-ageing on meat quality, oxidative stability, and release of free amino acids (FAAs) in striploins from dairy-crossbred yearlings and 2-year-old steers (n = 12 each group) over 21 days of in-bag dry-ageing. Dry-ageing increased weight losses, with higher % drying rates in yearling meat during dry-ageing, likely due to the smaller loin size and lower intramuscular fat content (P < 0.05). Yearling meat showed greater (P < 0.05) decreases in moisture content, but both meats reached similar moisture levels by day 21. pH values increased with dry-ageing with variations at different ageing times. Dry-ageing reduced a*, b*, and chroma while increasing L* and hue angles on day 21 (P < 0.05), likely due to dehydration and lipid oxidation (higher TBARS, P < 0.05) after 14 days, especially in yearling meat. The decreased levels (P < 0.05) of some monounsaturated fatty acids and conjugated linoleic acid cis-9, trans-11 were likely linked with lipid oxidation. Total levels of FAAs and essential amino acids increased significantly, especially within the first 7 days, with distinct patterns between the two meats. Dry-aged yearling meat contained more FAAs associated with sweat taste (e.g., glutamine and glycine) and fewer FAAs associated with bitter taste (e.g., phenylalanine and tyrosine). Carnosine levels varied and significantly increased after 21 days. Dry-ageing demonstrated distinct effects on dehydration, lipid oxidation, and release of FAAs in meat from yearlings compared with 2-year-old steers, which can be tailored to develop high-quality beef products with unique flavours.
本研究测定了在21天的袋中干龄化过程中,干龄化对奶牛杂交一岁牛和两岁阉牛(每组12头)的肉质、氧化稳定性和游离氨基酸(FAA)释放的影响。干燥老化增加了体重损失,一岁牛在干燥老化期间的干燥率较高,这可能是由于其腰部较小和肌肉内脂肪含量较低(P <0.05)。一岁肉的水分含量下降较多(P < 0.05),但到第 21 天时两种肉的水分含量相近。干制老化在第 21 天降低了 a*、b* 和色度,同时增加了 L* 和色调角(P < 0.05),这可能是由于 14 天后脱水和脂质氧化(TBARS 增加,P < 0.05),尤其是一岁羔羊肉。一些单不饱和脂肪酸和共轭亚油酸顺式-9、反式-11 水平的降低(P <0.05)可能与脂质氧化有关。FAAs和必需氨基酸的总含量显著增加,特别是在最初的7天内,两种肉类之间有明显的差异。干制一岁羔羊肉含有更多与汗味相关的 FAAs(如谷氨酰胺和甘氨酸),而与苦味相关的 FAAs(如苯丙氨酸和酪氨酸)较少。肌肽的含量各不相同,21 天后显著增加。与 2 岁的阉牛相比,干龄化对一岁阉牛肉的脱水、脂质氧化和 FAAs 的释放有明显的影响,可用于开发具有独特风味的优质牛肉产品。
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引用次数: 0
Empowering informed choices: How computer vision can assists consumers in making decisions about meat quality 赋予知情选择权:计算机视觉如何帮助消费者做出有关肉类质量的决定。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-21 DOI: 10.1016/j.meatsci.2024.109675
Guilherme L. Menezes , Dante T. Valente Junior , Rafael E.P. Ferreira , Dario A.B. Oliveira , Julcimara A. Araujo , Marcio Duarte , Joao R.R. Dorea
Consumers often find it challenging to assess meat sensory quality, influenced by tenderness and intramuscular fat (IMF). This study aims to develop a computer vision system (CVS) using smartphone images to classify beef and pork steak tenderness (1), predicting shear force (SF) and IMF content (2), and performing a comparative evaluation between consumer assessments and the method's output (3). The dataset consisted of 924 beef and 514 pork steaks (one image per steak). We trained a deep neural network for image classification and regression. The model achieved an F1-score of 68.1 % in classifying beef as tender. After re-categorizing the dataset into ‘tender’ and ‘tough’, the F1-score for identifying tender increased to 76.6 %. For pork loin tenderness, the model achieved an F1-score of 81.4 %. This score slightly improved to 81.5 % after re-categorization into two classes. The regression models for predicting SF and IMF in beef steak achieved an R2 value of 0.64 and 0.62, respectively, with a root mean squared prediction error (RMSEP) of 16.9 N and 2.6 %. For pork loin, the neural network predicted SF with an R2 value of 0.76 and an RMSEP of 9.15 N, and IMF with an R2 value of 0.54 and an RMSEP of 1.22 %. In 1000 paired comparisons, the neural network correctly identified the more tender beef steak in 76.5 % of cases, compared to a 46.7 % accuracy rate for human assessments. These findings suggest that CVS can provide a more objective method for evaluating meat tenderness and IMF before purchase, potentially enhancing consumer satisfaction.
消费者在评估肉类感官质量时往往会发现,肉类的嫩度和肌内脂肪(IMF)会影响肉类的感官质量。本研究旨在开发一种计算机视觉系统(CVS),利用智能手机图像对牛肉和猪肉牛排的嫩度进行分类(1),预测剪切力(SF)和肌内脂肪含量(2),并对消费者的评价和该方法的输出结果进行比较评估(3)。数据集包括 924 块牛肉牛排和 514 块猪肉牛排(每块牛排一张图片)。我们训练了一个深度神经网络来进行图像分类和回归。该模型在将牛肉分类为嫩肉方面取得了 68.1 % 的 F1 分数。将数据集重新分类为 "嫩 "和 "韧 "后,识别嫩度的 F1 分数提高到 76.6%。对于猪里脊肉的嫩度,模型的 F1 分数为 81.4%。在重新分为两类后,这一分数略微提高到 81.5%。预测牛扒 SF 和 IMF 的回归模型的 R2 值分别为 0.64 和 0.62,均方根预测误差 (RMSEP) 分别为 16.9 N 和 2.6 %。对于猪里脊肉,神经网络预测 SF 的 R2 值为 0.76,RMSEP 为 9.15 N,预测 IMF 的 R2 值为 0.54,RMSEP 为 1.22 %。在 1000 次配对比较中,神经网络在 76.5% 的情况下正确识别出了更嫩的牛排,而人工评估的准确率为 46.7%。这些研究结果表明,CVS 可以为购买前评估肉质嫩度和 IMF 提供更客观的方法,从而提高消费者的满意度。
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引用次数: 0
Visible-NIR spectroscopic authentication assay for the classification of lamb meat according to pasture-finishing duration 根据牧场饲养时间对羊肉进行分类的可见-近红外光谱鉴定分析。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-17 DOI: 10.1016/j.meatsci.2024.109670
Lucille Rey-Cadilhac, Donato Andueza, Sophie Prache
Spectral analysis of meat combined with chemometric analysis has been identified as a promising tool for authenticating livestock-animal diets. The objectives of this study were (i) to determine whether the visible-NIR spectrum of perirenal adipose tissue (PAT) and caudal adipose tissue (CAT) can reliably discriminate lambs pasture-finished for different durations before slaughter, and (ii) to analyze the kinetics of appearance and stabilization of the visible-NIR spectrum-based pasture signature in PAT and CAT. Four groups of 50–55 lambs were used over three years: lambs finished on lucerne pasture for 0 (L0, concentrate-fed in stall), 21 (L21), 42 (L42) and 63 (L63) days before slaughter. Partial least squares discriminant analysis was applied on PAT or CAT visible-NIR spectra to discriminate the groups. No one adipose tissue reliably discriminated the four groups, with less than 62 % lambs correctly classified. However, visible-NIR spectroscopy was able to discriminate stall-fed (L0) from pasture-finished (L21 + L42 + L63) lambs, with an accuracy of 93.8 % and 87.5 % lambs correctly classified based on PAT and CAT spectra, respectively. The lucerne pasture fingerprint (or signature) on visible-NIR spectrum appeared between 0 and 42 days in more than 95 % of lambs. It stabilized between 42 and 63 days in CAT, but had not stabilized within the range of grazing durations pre-slaughter explored in PAT. Further research into shorter and longer pasture-finishing durations could help determine more precisely the time required for the pasture signature to appear and stabilize in animal tissues.
肉的光谱分析与化学计量分析相结合,被认为是鉴定家畜日粮的一种有前途的工具。本研究的目的是:(i) 确定肾周脂肪组织(PAT)和尾部脂肪组织(CAT)的可见-近红外光谱是否能可靠地区分屠宰前不同时间段牧草加工的羔羊;(ii) 分析 PAT 和 CAT 中基于可见-近红外光谱的牧草特征的出现和稳定动力学。在三年中使用了四组 50-55 只羔羊:屠宰前在苜蓿草地上完成 0 天(L0,圈养精饲料)、21 天(L21)、42 天(L42)和 63 天(L63)的羔羊。对 PAT 或 CAT 可见光-近红外光谱采用偏最小二乘法判别分析来区分各组。没有一种脂肪组织能可靠地区分四个组别,只有不到 62% 的羔羊能被正确分类。然而,可见-近红外光谱分析能够区分厩肥(L0)羔羊和牧草加工(L21 + L42 + L63)羔羊,根据 PAT 和 CAT 光谱正确分类的准确率分别为 93.8 % 和 87.5 %。可见光-近红外光谱上的苜蓿草指纹(或特征)在 0 至 42 天期间出现在 95% 以上的羔羊身上。在 CAT 中,苜蓿草指纹在 42 到 63 天之间趋于稳定,但在 PAT 中,苜蓿草指纹在屠宰前的放牧时间范围内并未趋于稳定。进一步研究更短和更长的牧草结束持续时间有助于更准确地确定牧草特征在动物组织中出现和稳定所需的时间。
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引用次数: 0
Tailoring meat products for the elderly: A comprehensive review 为老年人量身定制肉制品:全面回顾
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-16 DOI: 10.1016/j.meatsci.2024.109669
Mustafa M. Farouk , Renyu Zhang , David I. Jenkinson , Carolina E. Realini

The population of the elderly is projected to grow significantly in most of the developed countries in the near future. This should earn this demographic the title of ‘Consumer of the future’. Meat has high quality proteins and essential vitamins which are important for all demographics but more so for the elderly due to their susceptibility to sarcopenia. This review explored the landscape of meat research and product development that meets the distinctive requirements of the elderly, drawing from published refereed articles, industry reports, and unpublished grey literature. The review emphasised the importance of understanding the evolving dietary and sensory requirements associated with ageing and how they intersect with meat consumption. One notable aspect observed is the diversity within the elderly population, which underscores the necessity for tailored approaches in meat product development to cater to the varying preferences and nutritional needs of different individuals. This review also highlights the ongoing efforts in developing meat-based products that span a spectrum of consistencies, ranging from solid to liquid forms, to accommodate the diverse needs of elderly consumers. Despite these advancements, the review found a discrepancy between the pace of meat product development for the elderly and the rapid advancements in kitchen-level technologies and suggests that further research is needed to bridge this gap and align product innovation with emerging technological trends.

预计在不久的将来,大多数发达国家的老年人口将大幅增长。这将为这一人口群体赢得 "未来消费者 "的称号。肉类含有优质蛋白质和必需维生素,这对所有人群都很重要,但由于老年人易患肌肉疏松症,因此对他们来说更为重要。本综述从已发表的参考文章、行业报告和未发表的灰色文献中,探讨了肉类研究和产品开发的现状,以满足老年人的独特需求。综述强调了了解与老龄化相关的不断变化的饮食和感官要求以及这些要求如何与肉类消费相联系的重要性。其中一个值得注意的方面是老年人口的多样性,这突出表明在肉类产品开发中必须采用量身定制的方法,以满足不同个体的不同偏好和营养需求。本综述还强调了为满足老年消费者的不同需求,在开发从固态到液态等各种稠度的肉类产品方面所做的不懈努力。尽管取得了这些进步,但综述发现,老年人肉类产品的开发速度与厨房级技术的快速发展之间存在差距,并建议需要进一步研究以缩小这一差距,使产品创新与新兴技术趋势保持一致。
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引用次数: 0
Flavor characterization of pork cuts in Chalu black pigs using multi-omics analysis 利用多组学分析确定茶陵黑猪猪肉风味特征
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-16 DOI: 10.1016/j.meatsci.2024.109668
Yingying Zhang , Yuduan Diao , Sayed Haidar Abbas Raza , Ji Huang , Hongyang Wang , Weilong Tu , Jiajie Zhang , Jieke Zhou , Yongsong Tan
The study investigated the flavor variations in four different fresh pork cuts (longissimus thoracis, LT; trapezius muscle, TM; hamstring muscle, HM; Pork Belly, PB) from Chalu black pigs (ten castrated boars) using multi-omics techniques. The research also explored the influence of muscle fiber type on the flavor profiles of these cuts. Results from quantitative real-time PCR (qRT-PCR) indicated significant differences in muscle fiber type across the four pork cuts in various anatomical locations. Each cut exhibited distinctive volatile organic compounds (VOCs) profiles, with HM displaying a sweet and fruity green flavor, LT showcasing a fatty and nutty taste, PB presenting a fresh, citrusy, and green flavor, and TM offering a floral and bitter note. Variations in fatty acid carbon number and saturation were observed among the cuts, with HM, LT, and PB being rich in fatty acids with C16–18, C19–21, and 3 double bonds, respectively. The metabolites specific to each cut were found to play key roles in different metabolic pathways, such as protein-related pathways for HM, arginine biosynthesis for LT, lysine biosynthesis for PB, and D-arginine and D-ornithine metabolism for TM. Differentially expressed genes (DEGs) were associated with amino acid metabolism for HM, glycolysis/gluconeogenesis for LT, and cellular aromatic compound organization for PB. Notably, HM and PB displayed unique flavor characteristics, while TM exhibited relatively neutral features. The study also identified correlations among VOCs, muscle fiber type, lipids, metabolites, and gene patterns specific to each cut, highlighting the complex interplay of factors influencing pork flavor.
该研究利用多组学技术调查了四种不同新鲜猪肉(胸长肌,LT;斜方肌,TM;腿筋肌,HM;猪肚,PB)的风味变化,这些猪肉来自 Chalu 黑猪(10 头阉公猪)。研究还探讨了肌肉纤维类型对这些肉类风味的影响。定量实时 PCR (qRT-PCR) 的结果表明,四种猪肉不同解剖位置的肌肉纤维类型存在显著差异。每种猪肉都呈现出独特的挥发性有机化合物(VOCs)特征,其中 HM 表现出甜美的绿色果味,LT 表现出脂肪和坚果味,PB 表现出新鲜、柑橘和绿色风味,而 TM 则表现出花香和苦味。不同切片的脂肪酸碳数和饱和度存在差异,HM、LT 和 PB 分别富含 C16-18、C19-21 和 3 个双键的脂肪酸。研究发现,各切口特有的代谢物在不同的代谢途径中发挥着关键作用,如 HM 的蛋白质相关途径、LT 的精氨酸生物合成、PB 的赖氨酸生物合成以及 TM 的 D-精氨酸和 D-鸟氨酸代谢。差异表达基因(DEGs)与 HM 的氨基酸代谢、LT 的糖酵解/葡萄糖生成和 PB 的细胞芳香化合物组织有关。值得注意的是,HM 和 PB 表现出独特的风味特征,而 TM 则表现出相对中性的特征。该研究还确定了挥发性有机化合物、肌肉纤维类型、脂类、代谢物和基因模式之间的相关性,这些因素对猪肉风味的影响错综复杂。
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引用次数: 0
Improving quality and consumer acceptance of rabbit meat: Prospects and challenges 提高兔肉质量和消费者接受度:前景与挑战。
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-14 DOI: 10.1016/j.meatsci.2024.109660
Pavan Kumar , Neelesh Sharma , Lokesh Kumar Narnoliya , Akhilesh Kumar Verma , Pramila Umaraw , Nitin Mehta , Mohammad Rashedi Ismail-Fitry , Ubedullah Kaka , Goh Yong-Meng , Sun-Jin Lee , Awis Qurni Sazili
Rabbit meat is an excellent source of high-quality proteins, essential fatty acids, vitamins, and minerals, which can be further improved through various management, preslaughter, and post-slaughter interventions. Rabbit meat consumption is popular in certain regions of the world. The multidimensional role of rabbits as pet, pest, and laboratory animals, lack of proper knowledge among consumers towards the nutritive value of rabbit meat, animal welfare, and ethical issues, sustainable potential, undeveloped marketing, and processing chain, and price parity with available cheap meat and non-meat alternatives, are some constraints in the rabbit meat production. Increasing awareness of the nutritive value, positive health effects of rabbit meat consumption and production chain, development of processed meat products, and proper animal welfare compliance in rabbit production could improve consumer acceptance. The present manuscript reviewed various factors that affect the meat quality and consumer acceptance of rabbit meat for a more sustainable and viable source for global meat supply.
兔肉是优质蛋白质、必需脂肪酸、维生素和矿物质的极佳来源,可通过各种管理、宰前和宰后干预措施进一步提高兔肉的品质。兔肉消费在世界某些地区很受欢迎。兔子作为宠物、害虫和实验动物的多方面作用,消费者对兔肉的营养价值、动物福利和道德问题缺乏正确认识,可持续发展潜力,营销和加工链不发达,价格与现有的廉价肉类和非肉类替代品持平,这些都是兔肉生产的一些制约因素。提高人们对兔肉的营养价值、兔肉消费对健康的积极影响以及兔肉生产链的认识,开发兔肉加工产品,以及在兔肉生产中遵守适当的动物福利规定,可以提高消费者的接受程度。本手稿综述了影响兔肉质量和消费者接受度的各种因素,以期为全球肉类供应提供更可持续、更可行的来源。
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引用次数: 0
Carcass chilling method and electrical stimulation effects on meat quality and color in lamb 胴体冷却方法和电刺激对羊肉肉质和色泽的影响
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-14 DOI: 10.1016/j.meatsci.2024.109662
Maggie A. Mickelson , Robyn D. Warner , Rod J. Polkinghorne , Dennis L. Seman , Peter M. Crump , James R. Claus

Five treatments: Control (C), Pre-evisceration Electrical Stimulation (CES: 15 Hz, 700 mA, 500 μs pulse width, 45 s pulse duration), vascular Rinse & Chill® (RC), CES + RC (ESRC), and RC with ES after evisceration (RCES:15 Hz, 600 mA, 1000 μs, 45 s), were applied to 21 lambs each. After being excised from the carcass, muscles were vacuum packaged and aged (Longissimus lumborum, LL, 3 and 22 d postmortem; Semimembranosus, SM, 3 d postmortem). Temperature, pH, purge, cooking loss, color, Warner-Bratzler Shear Force (WBSF), and consumer sensory evaluations were determined. CES and ESRC resulted in the fastest drop in pH below 6 and ESRC had the lowest likelihood of cold shortening. Sensory tenderness in the ESRC LL was greater than C. No differences in WBSF were found among treatments. RC generally produced lamb with higher lightness (L*). The rapid drop in pH likely was responsible for the increased purge and cooking loss observed. Color was affected by the order of ES and RC application.

五种治疗方法:21 只羔羊分别接受了对照组(C)、开膛前电刺激(CES:15 Hz,700 mA,500 μs 脉宽,45 s 脉冲持续时间)、血管冲洗& Chill® (RC)、CES + RC (ESRC)和开膛后 RC 加 ES(RCES:15 Hz,600 mA,1000 μs,45 s)五种处理。从胴体上切除肌肉后,对肌肉进行真空包装和老化(腰长肌,LL,死后 3 天和 22 天;半膜肌,SM,死后 3 天)。对温度、pH 值、净化、蒸煮损失、颜色、华纳-布拉茨勒剪切力(WBSF)和消费者感官评价进行了测定。CES 和 ESRC 导致 pH 值最快降至 6 以下,ESRC 出现冷缩的可能性最低。ESRC LL 的感官嫩度高于 C。RC 生产的羔羊通常具有较高的光亮度(L*)。pH值的急剧下降可能是所观察到的净化和烹饪损失增加的原因。颜色受 ES 和 RC 施用顺序的影响。
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引用次数: 0
Light wavelengths that induce oxidation of oxymyoglobin in meat 诱导肉中氧肌红蛋白氧化的光波长
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-12 DOI: 10.1016/j.meatsci.2024.109664
Masahiro Waga, Koji Nakade

Light wavelengths that induce meat discoloration and the photoreceptors in the meat were studied. We investigated the effects of the light wavelength on the oxidation rate of myoglobin (Mb) by exposing Mb extracts or model solutions containing Mb to light at specific wavelengths with a bandwidth of 5 nm using a fluorescence spectrophotometer. The wavelengths examined comprised 385, 415, 445, 460, 490, 525, 555, 580, 605, 630,660, and 750 nm. In the Mb extracts, Mb oxidation was induced through exposure to the light at 445 and 580–605 nm; Mb was insensitive to light at 445 nm. Mitochondria, containing cytochrome a and cytochrome a3 with absorption peaks at 448 and 600 nm, and riboflavin with fluorescence at 450 nm were studied as 445 nm receptors. Mitochondria significantly oxidized Mb via cytochrome c oxidation through complex IV activity; however, no 445 nm-specific photo sensitivity effects were observed. In contrast, riboflavin increased the Mb oxidation rate induced via exposure to the light at 450 nm in a concentration-dependent manner (minimum concentration: 38.4 μg L−1). While native mitochondria did not show 445 nm-specific photosensitivity effects on Mb, supernatants of heated mitochondria conferred 445 nm-wavelength sensitivity to Mb. Riboflavin concentration in this supernatant was 182 ± 60 μg L−1. The Mb photosensitivity spectrum with 473 μg L−1 riboflavin had two peaks at 445 nm and 580 nm, which were similar to those of Mb extract. These results suggest that mitochondrial damage affects the meat discoloration through the release of cytochrome c and riboflavin.

我们研究了引起肉变色的光波长和肉中的光感受器。我们使用荧光分光光度计将肌红蛋白(Mb)提取物或含有肌红蛋白的模型溶液暴露在带宽为 5 纳米的特定波长的光线下,研究了光波长对肌红蛋白(Mb)氧化率的影响。检测的波长包括 385、415、445、460、490、525、555、580、605、630、660 和 750 nm。在 Mb 提取物中,445 和 580-605 纳米波长的光可诱导 Mb 氧化;Mb 对 445 纳米波长的光不敏感。线粒体含有细胞色素 a 和细胞色素 a3(吸收峰为 448 纳米和 600 纳米)以及核黄素(荧光峰为 450 纳米),作为 445 纳米受体进行研究。线粒体通过复合体 IV 的活性使细胞色素 c 氧化,从而明显氧化 Mb;但是,没有观察到 445 纳米特异性光敏感效应。相反,核黄素以浓度依赖的方式(最低浓度:38.4 μg L-1)提高了暴露在 450 纳米波长下的 Mb 氧化率。虽然原生线粒体对 Mb 不表现出 445 纳米波长的特异性光敏效应,但加热线粒体的上清液对 Mb 具有 445 纳米波长的敏感性。该上清液中核黄素的浓度为 182 ± 60 μg L-1。核黄素浓度为 473 μg L-1 的 Mb 光敏光谱在 445 nm 和 580 nm 处有两个峰值,与 Mb 提取物的峰值相似。这些结果表明,线粒体损伤通过释放细胞色素 c 和核黄素影响肉的褪色。
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引用次数: 0
Electrical stimulation to improve meat quality: Factors at interplay, underlying biochemical mechanisms and a second look into the molecular pathways using proteomics 电刺激改善肉质:相互影响的因素、潜在的生化机制以及利用蛋白质组学对分子途径的再认识
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-12 DOI: 10.1016/j.meatsci.2024.109663
Mohammed Gagaoua , Nuria Prieto , David L. Hopkins , Welder Baldassini , Yimin Zhang , Oscar López-Campos , Marzia Albenzio , Antonella della Malva

Ensuring consistent beef eating quality is paramount for meeting consumer demands and sustaining the meat industry. Electrical stimulation (ES) is a post-slaughter intervention used to accelerate post-mortem glycolysis, to avoid cold shortening, to control the tenderization rate of meat through sophisticated physical, chemical and biochemical mechanisms including proteolysis, to improve beef tenderness and to achieve normal pHu that might lead to positive impact on color. This review comprehensively examines the multifaceted effects of ES on beef quality, encompassing factors and settings influencing its efficacy and the underlying biochemical mechanisms revealed using traditional biochemistry methods. It then delves into the molecular pathways modulated by ES, as unveiled by muscle proteomics, aiming to provide a second look and an unprecedented understanding of the underlying biochemical mechanisms through an integrative proteomics analysis of low-voltage ES (LVES) proteomics studies. The proteins changing as a result of ES were gathered in a compendium of 67 proteins, from which 14 were commonly identified across studies. In-depth bioinformatics of this compendium allowed a comprehensive overview of the molecular signatures and interacting biochemical pathways behind electrically stimulated beef muscles. The proteins belong to interconnected molecular pathways including the ATP metabolic process and glycolysis, muscle structure and contraction, heat shock proteins, oxidative stress, proteolysis and apoptosis. Understanding the intricate interplay of molecular pathways behind ES could improve the efficiency of beef production, ensuring consistent meat quality and meeting consumer expectations. The integrative analysis approach performed in this study holds promise for the meat industry's sustainability and competitiveness.

要满足消费者的需求并维持肉类行业的发展,确保牛肉的食用品质始终如一至关重要。电刺激(ES)是一种屠宰后干预措施,用于加速死后糖酵解,避免冷缩,通过复杂的物理、化学和生化机制(包括蛋白质分解)控制肉质嫩化率,改善牛肉嫩度,并使 pH 值达到正常水平,从而对牛肉色泽产生积极影响。本综述全面探讨了 ES 对牛肉品质的多方面影响,包括影响其功效的因素和环境,以及使用传统生物化学方法揭示的潜在生物化学机制。然后,它深入探讨了肌肉蛋白质组学揭示的 ES 所调节的分子通路,旨在通过对低电压 ES(LVES)蛋白质组学研究的综合蛋白质组学分析,对潜在的生化机制进行第二次审视和前所未有的了解。因 ES 而发生变化的蛋白质汇集成一个包含 67 个蛋白质的简编,其中 14 个蛋白质在不同研究中被共同鉴定。通过对该简编进行深入的生物信息学研究,可以全面了解电刺激牛肉肌肉背后的分子特征和相互作用的生化途径。这些蛋白质属于相互关联的分子通路,包括 ATP 代谢过程和糖酵解、肌肉结构和收缩、热休克蛋白、氧化应激、蛋白分解和细胞凋亡。了解 ES 背后错综复杂的分子通路相互作用可提高牛肉生产效率,确保肉质稳定,满足消费者的期望。本研究采用的综合分析方法有望提高肉类行业的可持续性和竞争力。
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引用次数: 0
Influence of Capsicum chinense concentration and salt varieties on the quality attributes of Kilishi, a sundried beef jerky 辣椒浓度和盐品种对风干牛肉干 Kilishi 质量属性的影响
IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-09-11 DOI: 10.1016/j.meatsci.2024.109653
Kazeem D. Adeyemi , Rasheed O. Sulaimon , Hakeem Ishola , Rafiat M. Shittu , Feranmi J. Olaniran , Jamiu O. Jimoh , Halimat O. Akinola , Ahmed O. Rasheed , Yusuf Ibn. Yusuf , Abdulfatai Oluwasola , Bukunmi M. Olabisi

The impact of Capsicum chinense concentration and salt varieties on cholesterol oxides, physicochemical properties, microbial profiles and organoleptic attributes of Kilishi, a sundried beef jerky, was assessed. Kilishi (KL) was prepared from sundried strips of Biceps femoris and marinated with either 2 % Sodium chloride (NaCl) + 7 % fresh Capsicum chinense (CC) (KL−1), 1 % NaCl + 1 % Potassium chloride (KCl) + 7 % CC (KL−2), 1 % NaCl + 1 % Potassium citrate (C6H5K3O7) + 7 % CC (KL−3), 1 % NaCl + 14 % CC (KL-4), 1 % KCl + 14 % CC (KL-5) or 1 % C6H5K3O7 + 14 % CC (KL-6), and stored at 29 ± 1 °C for 90 d. The partial or total replacement of NaCl lowered (P < 0.05) the Na content in KL. The KL samples treated with 14 % CC had lower (P < 0.05) 25-hydroxy cholesterol, cholesta-3,5-dien-7-one, carbonyl, pH, malondialdehyde, and lightness and greater (P < 0.05) redness and Lactobacillus counts than those treated with 7 % CC. The chemical composition, sensory scores and water activity were unaffected by the additives. The taste, flavor, and overall acceptance scores of KL decreased (P < 0.05) after 30 days of storage. The substitution of KCl and C6H5K3O7 for NaCl and the increase in CC concentration from 7 to 14 % lowered the Na content and selected cholesterol oxides, respectively, without impairing the organoleptic traits of Kilishi.

本研究评估了辣椒的浓度和盐的种类对干牛肉干(Kilishi)的胆固醇氧化物、理化性质、微生物特征和感官特性的影响。干牛肉干(KL)由晒干的股二头肌条制成,用 2 % 氯化钠(NaCl)+ 7 % 新鲜辣椒(CC)(KL-1)、1 % 氯化钠+ 1 % 氯化钾(KCl)+ 7 % CC(KL-2)腌制、1 % NaCl + 1 % 柠檬酸钾 (C6H5K3O7) + 7 % CC (KL-3)、1 % NaCl + 14 % CC (KL-4)、1 % KCl + 14 % CC (KL-5) 或 1 % C6H5K3O7 + 14 % CC (KL-6),并在 29 ± 1 °C 下储存 90 d。NaCl 的部分或全部替代降低了 KL 中的 Na 含量(P < 0.05)。与使用 7% CC 处理的 KL 样品相比,使用 14% CC 处理的 KL 样品的 25-羟基胆固醇、胆甾-3,5-二烯-7-酮、羰基、pH 值、丙二醛和亮度更低(P <0.05),红度和乳酸杆菌数量更高(P <0.05)。添加剂对化学成分、感官评分和水活性没有影响。贮藏 30 天后,KL 的口感、风味和总体接受度评分下降(P < 0.05)。用氯化钾和 C6H5K3O7 替代氯化钠,以及将 CC 的浓度从 7% 提高到 14%,分别降低了钠的含量和所选胆固醇氧化物的含量,但没有影响脆皮的感官特征。
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Meat Science
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