Pub Date : 2023-04-24DOI: 10.52794/hujpharm.1192921
Nisha Shri CHENGAMA RAJU, Tee SİEW YİNG
Moringa oleifera has many therapeutic benefits one out of it is anti-cancer property. Therefore, many researchers have been screening the therapeutic potential of Moringa oleifera. The main objective of this study was to screen and explore the angiogenesis inhibition potential of Moringa oleifera pod extracts. With the aim of screening anti-angiogenic potential, extracts of Moringa oleifera pods were prepared by decoction method. The extracts were subjected to preliminary phytochemical screening to identify the nature of phytochemicals present in the pods. In-ovo chorioallantoic membrane assay was chosen to achieve the objective of the study. Water-soluble extractive value (15.00% w/w) was higher than that of alcohol-soluble extractive value (3.89% w/w), indicating that the Moringa oleifera pods have more water-soluble constituents. Qualitative phytochemical screening revealed presence of flavonoids. Angiogenesis inhibition effect was studied and compared with sunitinib. Statistical analysis revealed highest anti-angiogenesis activity in 100% methanolic extract. Least effect was observed in 50% aqueous extract. Anti-angiogenic potential of 100% methanolic extract was statistically significant when compared with other study groups. It is concluded that Moringa oleifera pods exert anti-angiogenic potential and more intensified and diversified studies are needed to enable a thorough investigation of this plant components in anti-cancer treatment.
{"title":"Anti-Angiogenesis Screening of Moringa oleifera Pod Extracts by In-Ovo Chorioallantoic Membrane (CAM) Assay","authors":"Nisha Shri CHENGAMA RAJU, Tee SİEW YİNG","doi":"10.52794/hujpharm.1192921","DOIUrl":"https://doi.org/10.52794/hujpharm.1192921","url":null,"abstract":"Moringa oleifera has many therapeutic benefits one out of it is anti-cancer property. Therefore, many researchers have been screening the therapeutic potential of Moringa oleifera. The main objective of this study was to screen and explore the angiogenesis inhibition potential of Moringa oleifera pod extracts. With the aim of screening anti-angiogenic potential, extracts of Moringa oleifera pods were prepared by decoction method. The extracts were subjected to preliminary phytochemical screening to identify the nature of phytochemicals present in the pods. In-ovo chorioallantoic membrane assay was chosen to achieve the objective of the study. Water-soluble extractive value (15.00% w/w) was higher than that of alcohol-soluble extractive value (3.89% w/w), indicating that the Moringa oleifera pods have more water-soluble constituents. Qualitative phytochemical screening revealed presence of flavonoids. Angiogenesis inhibition effect was studied and compared with sunitinib. Statistical analysis revealed highest anti-angiogenesis activity in 100% methanolic extract. Least effect was observed in 50% aqueous extract. Anti-angiogenic potential of 100% methanolic extract was statistically significant when compared with other study groups. It is concluded that Moringa oleifera pods exert anti-angiogenic potential and more intensified and diversified studies are needed to enable a thorough investigation of this plant components in anti-cancer treatment.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134955056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.52794/hujpharm.1099683
Jivika Nai̇k, B. Kuchekar, A. Kuchekar, R. Pujari
Breast cancer has remained a serious health concern globally for women despite the healthcare advances which have enabled early diagnosis and treatment. Due to its metastatic ability and development of resistance to chemotherapies, triple-negative breast cancer is an extremely challenging subtype to treat. Targeted and optimized therapy is imperative as these tumors have higher recurrence rates than other types of breast cancers. This review is focused on the novel therapeutic strategies that have been proposed for the treatment of these aggressive cancers including alternative approaches like patient selection using biomarkers, metabolic reprogramming and development of smart drug delivery systems (SDDS) using targeted nanoparticles to treat the tumors as well as ensure prevention of recurrence. All of these approaches are aimed towards removing and treating the malignancies of triple-negative breast cancer (TNBC) and optimizing the therapies according to the patient cohorts. Further research is, however, necessary for the designing of an effective therapeutic regimen for patient sub-groups suffering from TNBC.
{"title":"Novel treatment strategies for Triple-negative breast cancers: A comprehensive review","authors":"Jivika Nai̇k, B. Kuchekar, A. Kuchekar, R. Pujari","doi":"10.52794/hujpharm.1099683","DOIUrl":"https://doi.org/10.52794/hujpharm.1099683","url":null,"abstract":"Breast cancer has remained a serious health concern globally for women despite the healthcare advances which have enabled early diagnosis and treatment. Due to its metastatic ability and development of resistance to chemotherapies, triple-negative breast cancer is an extremely challenging subtype to treat. Targeted and optimized therapy is imperative as these tumors have higher recurrence rates than other types of breast cancers. This review is focused on the novel therapeutic strategies that have been proposed for the treatment of these aggressive cancers including alternative approaches like patient selection using biomarkers, metabolic reprogramming and development of smart drug delivery systems (SDDS) using targeted nanoparticles to treat the tumors as well as ensure prevention of recurrence. All of these approaches are aimed towards removing and treating the malignancies of triple-negative breast cancer (TNBC) and optimizing the therapies according to the patient cohorts. Further research is, however, necessary for the designing of an effective therapeutic regimen for patient sub-groups suffering from TNBC.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81629445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-06DOI: 10.52794/hujpharm.1090673
Fadli Husain, Ysrafil Ysrafi̇l, Rizka Pusji Astuti Daud, Andi Nur Aisyah, Alfat Fadri, Syamsu Nur
Traditional medicine "Jamu" is becoming very popular used by the community as an alternative treatment that can minimize side effects. However, it is profitable for manufacturers who are not responsible for herbal products by adding medicinal chemicals (BKO) to traditional herbal products. The samples used in this study were samples of traditional herbal medicine coded A1, A2; B1, B2; C1, C2; D1, D2; E1, E2; and F1, F2, which were analyzed qualitatively and quantitatively using thin-layer chromatography (TLC) and UV-Vis spectrophotometry. Based on the results obtained from the analysis using the TLC method, the herbal samples with code A1 A2 had a staining profile, Rf value, and chromatographic peak that were almost the same as the comparison standard for sodium metamizole with levels of 51.99% (A1) and 53.06% (B). the herbal medicine samples with codes B1, B2, and C1, C2 had stain profiles, Rf values, and spectrophotometric chromatography peaks that were almost the same as the comparison standard for paracetamol with paracetamol levels contained in the “Jamu” samples of 81.31% (B1), 81.66% (B2), 89.59% (C1) and 81.62% (C2). The results obtained from this study can be concluded that the samples of traditional herbal products with codes A1 and A2 contain sodium metamizole with levels of >50%. Samples of traditional herbal products B1, B2, C1, and C2 contain paracetamol with paracetamol levels contained > 50%, while the traditional herbal medicine samples with codes D1, D2, E1, E2, F1, and F2 have been identified as containing no medicinal chemicals in the traditional herbal medicine samples.
{"title":"UV-Vis Spektrofotometrisi ile Geleneksel Bitkisel Ürünlerde (Jamu) Tıbbi Kimyasalların Doğrulanması","authors":"Fadli Husain, Ysrafil Ysrafi̇l, Rizka Pusji Astuti Daud, Andi Nur Aisyah, Alfat Fadri, Syamsu Nur","doi":"10.52794/hujpharm.1090673","DOIUrl":"https://doi.org/10.52794/hujpharm.1090673","url":null,"abstract":"Traditional medicine \"Jamu\" is becoming very popular used by the community as an alternative treatment that can minimize side effects. However, it is profitable for manufacturers who are not responsible for herbal products by adding medicinal chemicals (BKO) to traditional herbal products. The samples used in this study were samples of traditional herbal medicine coded A1, A2; B1, B2; C1, C2; D1, D2; E1, E2; and F1, F2, which were analyzed qualitatively and quantitatively using thin-layer chromatography (TLC) and UV-Vis spectrophotometry. Based on the results obtained from the analysis using the TLC method, the herbal samples with code A1 A2 had a staining profile, Rf value, and chromatographic peak that were almost the same as the comparison standard for sodium metamizole with levels of 51.99% (A1) and 53.06% (B). the herbal medicine samples with codes B1, B2, and C1, C2 had stain profiles, Rf values, and spectrophotometric chromatography peaks that were almost the same as the comparison standard for paracetamol with paracetamol levels contained in the “Jamu” samples of 81.31% (B1), 81.66% (B2), 89.59% (C1) and 81.62% (C2). The results obtained from this study can be concluded that the samples of traditional herbal products with codes A1 and A2 contain sodium metamizole with levels of >50%. Samples of traditional herbal products B1, B2, C1, and C2 contain paracetamol with paracetamol levels contained > 50%, while the traditional herbal medicine samples with codes D1, D2, E1, E2, F1, and F2 have been identified as containing no medicinal chemicals in the traditional herbal medicine samples.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"605 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90529123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-17DOI: 10.52794/hujpharm.1140957
Cemre Aydeğer, H. Eroğlu
Introduction: Wounds commonly occur in pathological situations in the body, and healing processes result successfully under normal conditions. These processes usually consist of four main phases, as haemostasis, inflammation, proliferation, and remodelling, and are generally described as the healing processes of skin wounds in the literature. However, it is known that all tissues have a unique healing pattern and the most separated from cutaneous tissue is central nervous tissue among all tissues. The difference is attributed that the central nervous system has cell differences and molecules. Thus, examination of healing in the central nervous system is so important. In accordance with this purpose, physiopathological events become after injury of nervous system, tried to give with light of current information in this review. Conclusion: However, wound healing is a complex process, and decades of research have provided more information about central nervous system healing. When considering the affected people by the injury to their central nervous system, it is important that shining light on healing mechanisms is very important.
{"title":"Physiopathology of Wound Healing in Central Nervous System","authors":"Cemre Aydeğer, H. Eroğlu","doi":"10.52794/hujpharm.1140957","DOIUrl":"https://doi.org/10.52794/hujpharm.1140957","url":null,"abstract":"Introduction: Wounds commonly occur in pathological situations in the body, and healing processes result successfully under normal conditions. These processes usually consist of four main phases, as haemostasis, inflammation, proliferation, and remodelling, and are generally described as the healing processes of skin wounds in the literature. However, it is known that all tissues have a unique healing pattern and the most separated from cutaneous tissue is central nervous tissue among all tissues. The difference is attributed that the central nervous system has cell differences and molecules. Thus, examination of healing in the central nervous system is so important. In accordance with this purpose, physiopathological events become after injury of nervous system, tried to give with light of current information in this review. \u0000Conclusion: However, wound healing is a complex process, and decades of research have provided more information about central nervous system healing. When considering the affected people by the injury to their central nervous system, it is important that shining light on healing mechanisms is very important.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86520913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ülkemizde her yıl 1500 ile 4500 kişiye tanısı konulan ALS ilerleyici kas fonksiyon kaybı ile kendini gösteren ölümcül bir nörodejeneratif hastalık olarak bilinmektedir. Günden güne bireylerin kendi temel ihtiyaçlarını dahi tek başına karşılayamadığı bu hastalığın patogenezinde glutamat eksitotoksisitesi ve genetik faktörler en önemli etmenler olarak görülmektedir. Birçok semptomatik ve tedaviye yönelik ilaç araştırmaları devam etse de tedavi protokolünde yalnızca FDA onaylı riluzol ve edaravon yer almaktadır. Bunun yanı sıra hem hastalığın önlenmesi ve tedavi edilmesi hem de semptomların iyileştirilmesi ve hastaların yaşam kalitesinin arttırılmasında hücre bazlı tedavilerden ve fitoterapiden de yararlanılmaktadır. Bu çalışma ile ALS hastalığının patogenezine, tedavisine ve tedavisindeki gelişmelere yer verilerek ilaçların tedavi potansiyelleri incelenmiş ve yeni çalışmalara yol göstermesi amacıyla bir değerlendirme hazırlanmıştır.
{"title":"Amyotrofik Lateral Skleroz Patofizyolojisi ve Tedavi Yaklaşımları","authors":"Zeynep Yıldırım, Dicle Naz Toktaş, Öznur Demir, Zülfiye Gül, Burcu Şen Utsukarçi","doi":"10.52794/hujpharm.1064372","DOIUrl":"https://doi.org/10.52794/hujpharm.1064372","url":null,"abstract":"Ülkemizde her yıl 1500 ile 4500 kişiye tanısı konulan ALS ilerleyici kas fonksiyon kaybı ile kendini gösteren ölümcül bir nörodejeneratif hastalık olarak bilinmektedir. Günden güne bireylerin kendi temel ihtiyaçlarını dahi tek başına karşılayamadığı bu hastalığın patogenezinde glutamat eksitotoksisitesi ve genetik faktörler en önemli etmenler olarak görülmektedir. Birçok semptomatik ve tedaviye yönelik ilaç araştırmaları devam etse de tedavi protokolünde yalnızca FDA onaylı riluzol ve edaravon yer almaktadır. Bunun yanı sıra hem hastalığın önlenmesi ve tedavi edilmesi hem de semptomların iyileştirilmesi ve hastaların yaşam kalitesinin arttırılmasında hücre bazlı tedavilerden ve fitoterapiden de yararlanılmaktadır. Bu çalışma ile ALS hastalığının patogenezine, tedavisine ve tedavisindeki gelişmelere yer verilerek ilaçların tedavi potansiyelleri incelenmiş ve yeni çalışmalara yol göstermesi amacıyla bir değerlendirme hazırlanmıştır.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"47 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72485907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.52794/hujpharm.1102486
Kenan Can Tok, Gökhun Çağatay Erbi̇l, Şeyda Yayla, Merve Eylül Kiymaci, M. M. Hürkul
In this study, the antibacterial activity of methanol and acetone extracts of Chlorella sp. was examined. The chemical contents of the extracts were clarified by GC/MS analysis. Antibacterial activity of Chlorella sp. extracts was determined as a minimum inhibitory concentration by broth microdilution method against Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213. It was found that methanol and acetone extracts of Chlorella sp. showed antibacterial activity against B. subtilis ATCC 6633 (625 µg/ml and 1250 µg/ml, respectively), E. faecalis ATCC 29212 (>5000 µg/ml and 1250 µg/ml, respectively), E. coli ATCC 25922 (>5000 µg/ml), P. aeruginosa ATCC 27853 (>5000 µg/ml), S. aureus ATCC 29213 (2500 µg/ml) at the specified concentrations. In the chemical analysis of the extracts, it was determined that the fatty acids were in high amounts, 33.22% and 40.41%, respectively, in the methanol and acetone extracts. Among the alternative methods to show activity against pathogenic microorganisms, algae can be a good natural resource. This study showed that Chlorella sp. contains high fatty acids and has potential as an antibacterial agent of natural origin.
{"title":"Chlorella sp. Kültürünün Ham Ekstrelerinin Kimyasal İçeriği ve Antibakteriyel Aktivitesi","authors":"Kenan Can Tok, Gökhun Çağatay Erbi̇l, Şeyda Yayla, Merve Eylül Kiymaci, M. M. Hürkul","doi":"10.52794/hujpharm.1102486","DOIUrl":"https://doi.org/10.52794/hujpharm.1102486","url":null,"abstract":"In this study, the antibacterial activity of methanol and acetone extracts of Chlorella sp. was examined. The chemical contents of the extracts were clarified by GC/MS analysis. Antibacterial activity of Chlorella sp. extracts was determined as a minimum inhibitory concentration by broth microdilution method against Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213. It was found that methanol and acetone extracts of Chlorella sp. showed antibacterial activity against B. subtilis ATCC 6633 (625 µg/ml and 1250 µg/ml, respectively), E. faecalis ATCC 29212 (>5000 µg/ml and 1250 µg/ml, respectively), E. coli ATCC 25922 (>5000 µg/ml), P. aeruginosa ATCC 27853 (>5000 µg/ml), S. aureus ATCC 29213 (2500 µg/ml) at the specified concentrations. In the chemical analysis of the extracts, it was determined that the fatty acids were in high amounts, 33.22% and 40.41%, respectively, in the methanol and acetone extracts. Among the alternative methods to show activity against pathogenic microorganisms, algae can be a good natural resource. This study showed that Chlorella sp. contains high fatty acids and has potential as an antibacterial agent of natural origin.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89731456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-28DOI: 10.52794/hujpharm.1181365
Ece Çobanoglu, S. Şenel
Bir ilacın dozaj şeklinden salım özelliklerinin incelenmesinde kullanılan in vitro salım testi sayesinde hem ilacın in vitro yararlanımı hakkında bilgi edinilmesi hem de diğer ürünlerle eşdeğerliğinin karşılaştırması mümkün olmaktadır. Doğru ve güvenilir sonuçlar elde edilmesi için uygun salım testi yönteminin ve uygun salım koşullarının (sıcaklık, salım ortamı, pH, karıştırma /akış hızı vb.) seçilmesi esastır. Farmakopelerde birçok dozaj şekli için in vitro salım testi yöntemleri ve koşulları tanımlanmış olmasına karşın hâlihazırda nanopartiküler ilaç taşıyıcı sistemler için bir test yöntemi farmakopelerde mevcut değildir. Bu derlemede, nanopartiküler sistemlerden ilaç salımının incelenmesinde kullanılan test yöntemleri (örnek alma ve ayırma, membran difüzyon, sürekli akış vb.) ve güncel uygulamalarından bahsedilecek ve birbirlerine olan üstünlükleri ve sakıncaları tartışılacaktır.
{"title":"Nanopartiküler İlaç Taşıyıcı Sistemlerinin İncelenmesinde Kullanılan İn Vitro Salım Testi Yöntemlerine Genel Bir Bakış","authors":"Ece Çobanoglu, S. Şenel","doi":"10.52794/hujpharm.1181365","DOIUrl":"https://doi.org/10.52794/hujpharm.1181365","url":null,"abstract":"Bir ilacın dozaj şeklinden salım özelliklerinin incelenmesinde kullanılan in vitro salım testi sayesinde hem ilacın in vitro yararlanımı hakkında bilgi edinilmesi hem de diğer ürünlerle eşdeğerliğinin karşılaştırması mümkün olmaktadır. Doğru ve güvenilir sonuçlar elde edilmesi için uygun salım testi yönteminin ve uygun salım koşullarının (sıcaklık, salım ortamı, pH, karıştırma /akış hızı vb.) seçilmesi esastır. Farmakopelerde birçok dozaj şekli için in vitro salım testi yöntemleri ve koşulları tanımlanmış olmasına karşın hâlihazırda nanopartiküler ilaç taşıyıcı sistemler için bir test yöntemi farmakopelerde mevcut değildir. Bu derlemede, nanopartiküler sistemlerden ilaç salımının incelenmesinde kullanılan test yöntemleri (örnek alma ve ayırma, membran difüzyon, sürekli akış vb.) ve güncel uygulamalarından bahsedilecek ve birbirlerine olan üstünlükleri ve sakıncaları tartışılacaktır.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"63 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72423065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-25DOI: 10.52794/hujpharm.1184841
Özden Tari, Adem Akkal
Son yıllarda yeni ilaç geliştirme çalışmaları kapsamında yapılan araştırmalarda, öksüz ilaçların çok önemli bir yerinin olduğu görülmektedir. Öksüz ilaçların geliştirilmesiyle, nadir hastalıkların tedavisinde kullanılacak yararlı yeni ilaçların elde edilmesi mümkün olabileceği gibi, etkinliği kanıtlanmış bu ilaçların, henüz tedavisi mümkün olmayan pek çok hastalığın tedavisi için de öncü olması beklenmektedir. Bu çalışmada, öksüz ilaçların, mevcut durumuna ilişkin araştırma ve değerlendirme yapılması amaçlanmıştır. Çalışma kapsamında yapılan literatür taramaları sonucunda, öksüz ilaçlar hakkında yaşanan son gelişmeler ve bu ilaçlarla ilgili araştırma-geliştirme çalışmalarının artmasını sağlayan yasa ve programlar detaylı olarak sunulmuştur. Öksüz ilaçlarla ilgili sunulan sayısal veriler, bu ilaçların yeni ilaç geliştirilmesindeki yerinin önemini göstermektedir.
{"title":"Öksüz İlaçlar ve Öksüz İlaçların Yeni İlaç Geliştirilmesindeki Yeri","authors":"Özden Tari, Adem Akkal","doi":"10.52794/hujpharm.1184841","DOIUrl":"https://doi.org/10.52794/hujpharm.1184841","url":null,"abstract":"Son yıllarda yeni ilaç geliştirme çalışmaları kapsamında yapılan araştırmalarda, öksüz ilaçların çok önemli bir yerinin olduğu görülmektedir. Öksüz ilaçların geliştirilmesiyle, nadir hastalıkların tedavisinde kullanılacak yararlı yeni ilaçların elde edilmesi mümkün olabileceği gibi, etkinliği kanıtlanmış bu ilaçların, henüz tedavisi mümkün olmayan pek çok hastalığın tedavisi için de öncü olması beklenmektedir. Bu çalışmada, öksüz ilaçların, mevcut durumuna ilişkin araştırma ve değerlendirme yapılması amaçlanmıştır. Çalışma kapsamında yapılan literatür taramaları sonucunda, öksüz ilaçlar hakkında yaşanan son gelişmeler ve bu ilaçlarla ilgili araştırma-geliştirme çalışmalarının artmasını sağlayan yasa ve programlar detaylı olarak sunulmuştur. Öksüz ilaçlarla ilgili sunulan sayısal veriler, bu ilaçların yeni ilaç geliştirilmesindeki yerinin önemini göstermektedir.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82646736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.52794/hujpharm.1070497
Rasmaizatul Akma Rosdi, M. D. Sul'ain, D. Darni̇s, Wan Rosli Wan Ishak
Smilax myosotiflora is one of the well-known plants in Malaysian folk medicines for its aphrodisiac effect. The leaves and fruits were also claimed able to treat syphilis and rheumatism. This article aims to systematically provide an overview on the traditional uses, pharmacology, toxicology and chemical constituents of S. myosotiflora. The e-searching on previous literature of the plant was conducted from its inception to the end 2021 according to the study’s criteria. The development of this review was based on the Cochrane Collaboration framework and PRISMA guidelines. As the result, it was found that over half of 43 eligible articles have discussed on its aphrodisiac attribution while others reported on the synergistic, sex reversal, antioxidant, anthelmintic activities and so forth. Two stilbenoid compounds are successfully isolated from the plant; trans-resveratrol and trans-3,3’,5,5’-tetrahydroxy-4’-methoxystilbene. Other than the detection of aurones and phytosterols, S. myosotiflora was delineated to be high in alkaloids, saponins and flavonoids. This systematic review can be a platform for other researchers to explore more on S. myosotiflora as it still has more pharmacology potential to be discovered. Further studies are required prior to the development of S. myosotiflora-based drugs for medicinal usage in the future.
{"title":"Traditional uses, pharmacology, toxicology and chemical constituents of an aphrodisiac plant, Smilax myosotiflora: A systematic review","authors":"Rasmaizatul Akma Rosdi, M. D. Sul'ain, D. Darni̇s, Wan Rosli Wan Ishak","doi":"10.52794/hujpharm.1070497","DOIUrl":"https://doi.org/10.52794/hujpharm.1070497","url":null,"abstract":"Smilax myosotiflora is one of the well-known plants in Malaysian folk medicines for its aphrodisiac effect. The leaves and fruits were also claimed able to treat syphilis and rheumatism. This article aims to systematically provide an overview on the traditional uses, pharmacology, toxicology and chemical constituents of S. myosotiflora. The e-searching on previous literature of the plant was conducted from its inception to the end 2021 according to the study’s criteria. The development of this review was based on the Cochrane Collaboration framework and PRISMA guidelines. As the result, it was found that over half of 43 eligible articles have discussed on its aphrodisiac attribution while others reported on the synergistic, sex reversal, antioxidant, anthelmintic activities and so forth. Two stilbenoid compounds are successfully isolated from the plant; trans-resveratrol and trans-3,3’,5,5’-tetrahydroxy-4’-methoxystilbene. Other than the detection of aurones and phytosterols, S. myosotiflora was delineated to be high in alkaloids, saponins and flavonoids. This systematic review can be a platform for other researchers to explore more on S. myosotiflora as it still has more pharmacology potential to be discovered. Further studies are required prior to the development of S. myosotiflora-based drugs for medicinal usage in the future.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73760549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-27DOI: 10.52794/hujpharm.1098619
Derouicha Matmour
Residual solvents are organic volatile impurities that can occur in the manufacture of Active Pharmaceutical Ingredients (APIs). Residual solvents analysis in pharmaceutical products is necessary not only because of they represent a potential risk for human health due to their toxicity and their undesirables side effects, but also because they may affect the physicochemical proprieties of pharmaceutical product. The main objective of this work was to analysis 29 residual solvents-impurities by Head Space Gas Chromatography with Flame Ionization Detector (HS-GC-FID) in seven samples of Ciprofloxacin Hydrochloride API, collected from seven pharmaceutical industries installed in Algeria. The GC was equipped with a flame-ionization detector and silica column coated with 1.8 µm layer of phase G43. The carrier gas was helium with a linear velocity of 35 cm/s and a split ratio of 1:5. The column temperature was 40 °C then it rised to 240 °C. The injection temperature was 140 °C and that of detector was 250 °C. Twenty-nine organic solvents belong to classes 1 and 2 were analyzed in seven samples of Ciprofloxacin Hydrochloride API whose control is mandatory because of their carcinogenic and intrinsic toxicity. Only five solvents were identified wich are Hexane, Toluene, Acetonitrile, Methanol and Dichloromethane in the different samples. All samples collected satisfied the test of identification, so, the confirmation and the quantification procedures weren’t realized. The HS-GC-FID technique used showed that the identified solvents differ from one sample to another of the same molecule. This showed that manufacturers didn’t often use the same solvents to produce the same API, which justifies that residual organic solvent tests weren’t usually mentioned in the specific monographs.
{"title":"Analysis of Residual Solvents-Impurities by HS-GC-FID: Case of Seven Samples of Ciprofloxacin API.","authors":"Derouicha Matmour","doi":"10.52794/hujpharm.1098619","DOIUrl":"https://doi.org/10.52794/hujpharm.1098619","url":null,"abstract":"Residual solvents are organic volatile impurities that can occur in the manufacture of Active Pharmaceutical Ingredients (APIs). Residual solvents analysis in pharmaceutical products is necessary not only because of they represent a potential risk for human health due to their toxicity and their undesirables side effects, but also because they may affect the physicochemical proprieties of pharmaceutical product. The main objective of this work was to analysis 29 residual solvents-impurities by Head Space Gas Chromatography with Flame Ionization Detector (HS-GC-FID) in seven samples of Ciprofloxacin Hydrochloride API, collected from seven pharmaceutical industries installed in Algeria. The GC was equipped with a flame-ionization detector and silica column coated with 1.8 µm layer of phase G43. The carrier gas was helium with a linear velocity of 35 cm/s and a split ratio of 1:5. The column temperature was 40 °C then it rised to 240 °C. The injection temperature was 140 °C and that of detector was 250 °C. Twenty-nine organic solvents belong to classes 1 and 2 were analyzed in seven samples of Ciprofloxacin Hydrochloride API whose control is mandatory because of their carcinogenic and intrinsic toxicity. Only five solvents were identified wich are Hexane, Toluene, Acetonitrile, Methanol and Dichloromethane in the different samples. All samples collected satisfied the test of identification, so, the confirmation and the quantification procedures weren’t realized. The HS-GC-FID technique used showed that the identified solvents differ from one sample to another of the same molecule. This showed that manufacturers didn’t often use the same solvents to produce the same API, which justifies that residual organic solvent tests weren’t usually mentioned in the specific monographs.","PeriodicalId":39138,"journal":{"name":"Hacettepe University Journal of the Faculty of Pharmacy","volume":"525 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83571730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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