Background: Marine biodiversity in the last few decades has been explored and utilized as marine natural products. The secondary metabolites produced by marine organisms are utilized by humans in various aspects of life. One of the main source of secondary metabolites is marine invertebrates, such as tunicate. Several previous studies have shown that many metabolites have been identified in the last 40 years.Objective: This study aimed to identify the metabolite compounds produced by tunicate-associated bacteria.Methods: The two isolates of bacteria associated with tunicate Polycarpa aurata, Bacillus wiedmannii and Virgibacillus salarius, obtained from the culture collection of Tropical Marine Biotechnology laboratory, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang were used as material in this study. This study was carried out in Integrated Laboratory Diponegoro University from July to September 2020. Laboratory experiment was conducted by culturing these bacteria in Zobell Marine Broth 2216 at room temperature. The incubated culture was then added with ethyl acetate in a ratio of 1: 2 and then the supernatant was separated and evaporated. Analysis of the tunicate-associated bacteria methanol extract was carried out using Gas Chromatography-Mass Spectrophotometry (GC-MS).Results: 3-N-Hexyl-Delta-9-Tetrahydrocannabinol compound (100%) was discovered by GC-MS analysis from the tunicate-associated bacteria B. wiedmannii and V. salarius extract.Conclusion: 3-N-Hexyl-Delta-9-Tetrahydrocannabinol compound is an isomer compound of Delta-9-Tetrahydrocannabinol (Δ9-THC) which needs further research on its application in medical and non-medical aspects.
背景:近几十年来，海洋生物多样性作为海洋天然产物被开发利用。海洋生物产生的次生代谢物被人类用于生活的各个方面。次生代谢物的主要来源之一是海洋无脊椎动物，如被囊动物。之前的几项研究表明，在过去的40年里，已经发现了许多代谢物。目的:鉴定被膜相关菌产生的代谢产物。方法:以三宝朗Diponegoro大学渔业与海洋科学学院热带海洋生物技术实验室培养集中分离的2株与黄颡鱼(Polycarpa aurata)有关的细菌wiedmannii芽孢杆菌和salarius芽孢杆菌为材料进行研究。本研究于2020年7月至9月在Diponegoro大学综合实验室进行。将这些细菌培养在佐贝尔海洋肉汤2216中，在室温下进行室内实验。然后将培养物按1:2的比例加入乙酸乙酯，然后分离上清并蒸发。采用气相色谱-质谱联用法(GC-MS)对被毛菌甲醇提取物进行分析。结果:通过气相色谱-质谱分析，从被毛相关菌B. wiedmannii和V. salarius提取物中发现3- n -己基- δ -9-四氢大麻酚化合物(100%)。结论:3- n-己基-德尔塔-9-四氢大麻酚化合物是德尔塔-9-四氢大麻酚(Δ9-THC)的异构体化合物，其在医疗和非医疗方面的应用有待进一步研究。
{"title":"IDENTIFICATION OF SECONDARY METABOLITE COMPOUNDS IN TUNICATE (Polycarpa aurata) ASSOCIATED BACTERIA","authors":"Sheila Raisa, Astika Widy Utomo, Rebriarina Hapsari, Endang Mahati","doi":"10.14710/dmj.v10i6.30103","DOIUrl":"https://doi.org/10.14710/dmj.v10i6.30103","url":null,"abstract":"Background: Marine biodiversity in the last few decades has been explored and utilized as marine natural products. The secondary metabolites produced by marine organisms are utilized by humans in various aspects of life. One of the main source of secondary metabolites is marine invertebrates, such as tunicate. Several previous studies have shown that many metabolites have been identified in the last 40 years.Objective: This study aimed to identify the metabolite compounds produced by tunicate-associated bacteria.Methods: The two isolates of bacteria associated with tunicate Polycarpa aurata, Bacillus wiedmannii and Virgibacillus salarius, obtained from the culture collection of Tropical Marine Biotechnology laboratory, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang were used as material in this study. This study was carried out in Integrated Laboratory Diponegoro University from July to September 2020. Laboratory experiment was conducted by culturing these bacteria in Zobell Marine Broth 2216 at room temperature. The incubated culture was then added with ethyl acetate in a ratio of 1: 2 and then the supernatant was separated and evaporated. Analysis of the tunicate-associated bacteria methanol extract was carried out using Gas Chromatography-Mass Spectrophotometry (GC-MS).Results: 3-N-Hexyl-Delta-9-Tetrahydrocannabinol compound (100%) was discovered by GC-MS analysis from the tunicate-associated bacteria B. wiedmannii and V. salarius extract.Conclusion: 3-N-Hexyl-Delta-9-Tetrahydrocannabinol compound is an isomer compound of Delta-9-Tetrahydrocannabinol (Δ9-THC) which needs further research on its application in medical and non-medical aspects.","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123525037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-30DOI: 10.14710/dmj.v10i6.31010
E. Nugraheni, D. Ramadhani, M. Sariyanti, Ety Febrianti
Background. Dengue Hemorrhagic Fever (DHF) is infection caused by Dengue Virus. Failure of vascularization is a main symptom of Dengue Hemorrhagic Fever inducing mediator secretion by an immune cell. FcgRIIA and CCL2 have a significant role in dengue pathogenesis and possibility in having a chance to cause dengue with a worse manifestation. Objective. Analysis of bio-informatic structure, function and expression of FcgRIIA gene. Methods. Insilico analysis used NCBI database to find position and sequences. Analysis mutant use SNO and OMIM. Protein prediction withUniprot. Result. FcgRIIA human with access number of NM_001136219 by a length of 2429 bp has its full name as Fc Fragment of IgG receptor IIa, located in 1q23.3 chromosom. analyzed mutation was rs1801274 with type of missense protein residue function experiencing a change from Histidin (H) turning into Arginin (R) with allele of wild-type A and becoming G amino acid position of 166. there was structural difference of FcgRIIA gene in wild type and mutant. Conclusion. Gene FcγRIIA is a play a role of pathogenesis of dengue infection. Mutation in FcγRIIA have polymorfisme at Dengue Hemorrage Fever
{"title":"Insilico Analysis of Codon 131 Polymorphism in FcγRIIA Gene and it is Association with Clinical Symptoms Persistence of Dengue Patients","authors":"E. Nugraheni, D. Ramadhani, M. Sariyanti, Ety Febrianti","doi":"10.14710/dmj.v10i6.31010","DOIUrl":"https://doi.org/10.14710/dmj.v10i6.31010","url":null,"abstract":"Background. Dengue Hemorrhagic Fever (DHF) is infection caused by Dengue Virus. Failure of vascularization is a main symptom of Dengue Hemorrhagic Fever inducing mediator secretion by an immune cell. FcgRIIA and CCL2 have a significant role in dengue pathogenesis and possibility in having a chance to cause dengue with a worse manifestation. Objective. Analysis of bio-informatic structure, function and expression of FcgRIIA gene. Methods. Insilico analysis used NCBI database to find position and sequences. Analysis mutant use SNO and OMIM. Protein prediction withUniprot. Result. FcgRIIA human with access number of NM_001136219 by a length of 2429 bp has its full name as Fc Fragment of IgG receptor IIa, located in 1q23.3 chromosom. analyzed mutation was rs1801274 with type of missense protein residue function experiencing a change from Histidin (H) turning into Arginin (R) with allele of wild-type A and becoming G amino acid position of 166. there was structural difference of FcgRIIA gene in wild type and mutant. Conclusion. Gene FcγRIIA is a play a role of pathogenesis of dengue infection. Mutation in FcγRIIA have polymorfisme at Dengue Hemorrage Fever","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127202646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-30DOI: 10.14710/dmj.v10i6.30190
Rahma Khusnul Khotimah, B. Pramono, Suharto Suharto, Yuli Trisetiyono
Background: Infertility is an inability of a spouse to get pregnant within twelve months seeking pregnancy without using any contraception. The duration of infertility affects the chance to get pregnant.Objective: To identify the factors causing infertility patient came late to Polyclinic Merpati RSUP Dr. KariadiMethods: This study was an observational descriptive with a cross-sectional design. The data were obtained from medical records and questionnaires. Twenty-six respondents were infertility patients who met the inclusion and exclusion criteria and came to Polyclinic Merpati RSUP Dr. Kariadi in September 2020.Results: The number of infertility patients in September 2020 was 76 patients, 72,4% of them came to Polyclinic Merpati late. Most people came after being referred from the previous doctors. Three factors causing infertility patients came late were economic factors, a social factor, and another difficulty.Conclusion: Factors causing the late coming of infertility patients were economic factors, a social factor, and another difficulty
背景:不孕是指配偶在未使用任何避孕措施的情况下在12个月内无法怀孕。不孕的持续时间会影响怀孕的机会。目的:探讨在Merpati RSUP综合诊所就诊的晚期不孕症患者的病因。方法:本研究采用横断面设计的观察性描述性研究。数据来自医疗记录和问卷调查。26名受访者是符合纳入和排除标准的不孕症患者，并于2020年9月来到Merpati RSUP Dr. Kariadi。结果:2020年9月不孕不育患者76例，其中晚来Merpati分院的占71.4%。大多数人都是从以前的医生那里转过来的。导致不孕患者晚来的三个因素分别是经济因素、社会因素和另一个困难因素。结论:造成不孕患者晚育的因素有经济因素、社会因素和困难因素
{"title":"Why Infertility Patients Came Late to Infertility Clinics (Study at Kariadi General Hospital)","authors":"Rahma Khusnul Khotimah, B. Pramono, Suharto Suharto, Yuli Trisetiyono","doi":"10.14710/dmj.v10i6.30190","DOIUrl":"https://doi.org/10.14710/dmj.v10i6.30190","url":null,"abstract":"Background: Infertility is an inability of a spouse to get pregnant within twelve months seeking pregnancy without using any contraception. The duration of infertility affects the chance to get pregnant.Objective: To identify the factors causing infertility patient came late to Polyclinic Merpati RSUP Dr. KariadiMethods: This study was an observational descriptive with a cross-sectional design. The data were obtained from medical records and questionnaires. Twenty-six respondents were infertility patients who met the inclusion and exclusion criteria and came to Polyclinic Merpati RSUP Dr. Kariadi in September 2020.Results: The number of infertility patients in September 2020 was 76 patients, 72,4% of them came to Polyclinic Merpati late. Most people came after being referred from the previous doctors. Three factors causing infertility patients came late were economic factors, a social factor, and another difficulty.Conclusion: Factors causing the late coming of infertility patients were economic factors, a social factor, and another difficulty","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121391389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-30DOI: 10.14710/dmj.v10i6.31222
Dean Pandu Eko Sapto, D. Hermawati, Rr Mahayu Dewi Ariani, Farmaditya Eka Putra
Background: Mosquito coil smoke contains Allethrin that can increase the free radical effect. This process may result in the damage of the cell membranes that triggered by oxidative stress. Oxidative stress can affect spermatogenesis and decreasing the quality of spermatozoa, including the spermatozoa concentration. Beetroot contain flavonoid as an antioxidant which can counteract the free radicals in the body.Purpose: To identify the effect of beetroot juice in the spermatozoa concentration of Wistar rats that exposed by mosquito coil smoke.Method: This study used Post Test Only control group design. The samples were 28 male Wistar rats that divided into 4 groups. The Control Group(-) was a group without treatment. The Control Group(+) was only exposed by mosquito coil smoke. Group P1 was exposed by mosquito coil smoke and given 8 ml of beetroot juice. P2 group was exposed by mosquito coil smoke and given 16 ml of beetroot juice. For 56 days, on the 57th day all rats were terminated and spermatozoa concentrations were examined.Results: The average concentration of spermatozoa was: Group K(-)=20,00; Group K(+)=8,00; P1 group=12,40; P2 group=17,20. One Way ANOVA test found there were significant differences among the five treatment groups. Post-Hoc test found there were significant differences among K(-) with K(+), K(-) with P1, K(-) with P2, K(+) with P1, K(+) with P2 and P1 with P2.Conclusion: Beetroot juice administration increasing the spermatozoa concentration of male wistar rats that exposed by cigarette smoke.Keywords: Mosquito coil smoke, beetroot juice, spermatozoa concentration
{"title":"THE EFFECT OF BEETROOT JUICE (Beta vulgaris L.) ON THE CONCENTRATION OF SPERMATOZOA IN WISTAR RATS EXPOSED TO MOSQUITO COIL SMOKE","authors":"Dean Pandu Eko Sapto, D. Hermawati, Rr Mahayu Dewi Ariani, Farmaditya Eka Putra","doi":"10.14710/dmj.v10i6.31222","DOIUrl":"https://doi.org/10.14710/dmj.v10i6.31222","url":null,"abstract":"Background: Mosquito coil smoke contains Allethrin that can increase the free radical effect. This process may result in the damage of the cell membranes that triggered by oxidative stress. Oxidative stress can affect spermatogenesis and decreasing the quality of spermatozoa, including the spermatozoa concentration. Beetroot contain flavonoid as an antioxidant which can counteract the free radicals in the body.Purpose: To identify the effect of beetroot juice in the spermatozoa concentration of Wistar rats that exposed by mosquito coil smoke.Method: This study used Post Test Only control group design. The samples were 28 male Wistar rats that divided into 4 groups. The Control Group(-) was a group without treatment. The Control Group(+) was only exposed by mosquito coil smoke. Group P1 was exposed by mosquito coil smoke and given 8 ml of beetroot juice. P2 group was exposed by mosquito coil smoke and given 16 ml of beetroot juice. For 56 days, on the 57th day all rats were terminated and spermatozoa concentrations were examined.Results: The average concentration of spermatozoa was: Group K(-)=20,00; Group K(+)=8,00; P1 group=12,40; P2 group=17,20. One Way ANOVA test found there were significant differences among the five treatment groups. Post-Hoc test found there were significant differences among K(-) with K(+), K(-) with P1, K(-) with P2, K(+) with P1, K(+) with P2 and P1 with P2.Conclusion: Beetroot juice administration increasing the spermatozoa concentration of male wistar rats that exposed by cigarette smoke.Keywords: Mosquito coil smoke, beetroot juice, spermatozoa concentration","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132146276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14710/DMJ.V10I5.30184
Lidwina Vania Shaynaputri, Anindita Soetadji, D. Adespin, Stefani Candra Firmanti
Background: TB screening in children having close contact with smear-positive TB patients is urgent to conduct to improve the case detection rate and to achieve the “End TB” target. In fact, the implementation of screening in Indonesia is still inadequate.Objective: To analyze the factors associated with the non-adherence of TB screening in children aged <15 years with smear-positive TB patients’ close contact.Methods: A cross-sectional study was conducted in Semarang between February and August 2020. Subjects were children <15 years old with smear-positive TB patient's close contact. Data were collected from interviewed parents or guardians of the child using a structured questionnaire. Chi-square or Fisher's exact test analysis was used to identify the factor associated with non-adherence to TB screening in children.Results: A total of 84 children were recruited into this study. The proportion of children who did not undergo the screening is still high (86,9%), and there was a significant association with children's age (OR 13,556, 95% CI=3.135-58,613), parents' age (OR 41,143, 95% CI=4,025-420,604), parents’ education level (OR 0,114, 95%CI=0.027–0.477), distance to health facilities, knowledge level, perceived barriers, and cues to action. There was no significant association between children’s gender, economic level, perceived threats with non-adherence of screening.Conclusion: Children and parents’ age, education level, distance to health facilities, knowledge level, perception of barriers, and cues to action associated with the non-adherence of TB screening in children aged <15 years with smear-positive TB patients’ close contact.
{"title":"Factors Associated with the Non-Adherence of TB Screening in Children Aged <15 Years with Smear-Positive TB Patients’ Close Contact","authors":"Lidwina Vania Shaynaputri, Anindita Soetadji, D. Adespin, Stefani Candra Firmanti","doi":"10.14710/DMJ.V10I5.30184","DOIUrl":"https://doi.org/10.14710/DMJ.V10I5.30184","url":null,"abstract":"Background: TB screening in children having close contact with smear-positive TB patients is urgent to conduct to improve the case detection rate and to achieve the “End TB” target. In fact, the implementation of screening in Indonesia is still inadequate.Objective: To analyze the factors associated with the non-adherence of TB screening in children aged <15 years with smear-positive TB patients’ close contact.Methods: A cross-sectional study was conducted in Semarang between February and August 2020. Subjects were children <15 years old with smear-positive TB patient's close contact. Data were collected from interviewed parents or guardians of the child using a structured questionnaire. Chi-square or Fisher's exact test analysis was used to identify the factor associated with non-adherence to TB screening in children.Results: A total of 84 children were recruited into this study. The proportion of children who did not undergo the screening is still high (86,9%), and there was a significant association with children's age (OR 13,556, 95% CI=3.135-58,613), parents' age (OR 41,143, 95% CI=4,025-420,604), parents’ education level (OR 0,114, 95%CI=0.027–0.477), distance to health facilities, knowledge level, perceived barriers, and cues to action. There was no significant association between children’s gender, economic level, perceived threats with non-adherence of screening.Conclusion: Children and parents’ age, education level, distance to health facilities, knowledge level, perception of barriers, and cues to action associated with the non-adherence of TB screening in children aged <15 years with smear-positive TB patients’ close contact.","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125807801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14710/DMJ.V10I5.30115
Mochamad Haldi Tri Hutama, Windriyatna Windriyatna, D. Ngestiningsih, Rr Mahayu Dewi Ariani, Tyas Prihatiningsih
Background: Acrylic denture was an alternative to replace a lost tooth, and surrounding tissues have the part of the acrylic plate, which was frequently a place of Candida albicans proliferation. The liquid smoke has a chemical compound of phenol which produced antimicrobial and could hamper or destroy Candida albicans.Objectives: To identify the acrylic plate's effectiveness, soaking into liquid smoke to the growth of Candida albicans.Methods: This research was categorized into experimental research which exerted post-test control group research design. The total sample in this research was 25 samples, four treatment groups, and one control group. This treatment covered acrylic plate soaking into liquid smoke in specific concentration level 1%, 2%, 4%, and 6% to obstruct the growth of Candida albicans. Moreover, the statistic test was conducted through Kruskal-Wallis and continued to Post-Hoc test through Mann-Whitney theory.Findings: This research showed a significant difference between the amount of Candida albicans on the control group and liquid smoke group in concentrations 1%, 2%, 4%, and p-valuable = 0,005). Further, based on the Post-Hoc test result of Mann-Whitney, it referred significant difference between concentrations 1%, 2%, 4%, and 6% within sterile aquades (p-value = 0,008).Conclusions: The liquid smoke shared effective concentration which functioned to hamper Candida albicans on the acrylic plate. The concentration level of 6% was the most effective concentration to interfere Candida albicans on the acrylic plate. Furthermore, it was not found any significant difference between resistibility of liquid smoke to Candida albicans on an acrylic plate with concentration level of 1%-6% which could be the most effective way to obstruct Candida albicans on acrylic plate.Keywords : Acrylic plate, Liquid smoke, Candida albicans
{"title":"LIQUID SMOKE SUBMERSIVE EFFECTIVENESS OF ACRYLIC PLATE TO THE GROWTH OF CANDIDA ALBICANS","authors":"Mochamad Haldi Tri Hutama, Windriyatna Windriyatna, D. Ngestiningsih, Rr Mahayu Dewi Ariani, Tyas Prihatiningsih","doi":"10.14710/DMJ.V10I5.30115","DOIUrl":"https://doi.org/10.14710/DMJ.V10I5.30115","url":null,"abstract":"Background: Acrylic denture was an alternative to replace a lost tooth, and surrounding tissues have the part of the acrylic plate, which was frequently a place of Candida albicans proliferation. The liquid smoke has a chemical compound of phenol which produced antimicrobial and could hamper or destroy Candida albicans.Objectives: To identify the acrylic plate's effectiveness, soaking into liquid smoke to the growth of Candida albicans.Methods: This research was categorized into experimental research which exerted post-test control group research design. The total sample in this research was 25 samples, four treatment groups, and one control group. This treatment covered acrylic plate soaking into liquid smoke in specific concentration level 1%, 2%, 4%, and 6% to obstruct the growth of Candida albicans. Moreover, the statistic test was conducted through Kruskal-Wallis and continued to Post-Hoc test through Mann-Whitney theory.Findings: This research showed a significant difference between the amount of Candida albicans on the control group and liquid smoke group in concentrations 1%, 2%, 4%, and p-valuable = 0,005). Further, based on the Post-Hoc test result of Mann-Whitney, it referred significant difference between concentrations 1%, 2%, 4%, and 6% within sterile aquades (p-value = 0,008).Conclusions: The liquid smoke shared effective concentration which functioned to hamper Candida albicans on the acrylic plate. The concentration level of 6% was the most effective concentration to interfere Candida albicans on the acrylic plate. Furthermore, it was not found any significant difference between resistibility of liquid smoke to Candida albicans on an acrylic plate with concentration level of 1%-6% which could be the most effective way to obstruct Candida albicans on acrylic plate.Keywords : Acrylic plate, Liquid smoke, Candida albicans ","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117202531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14710/DMJ.V10I5.30046
Della Dafina Sekarsari, P. Ardhianto, Erwin Kresnoadi, M. A. Sobirin
Background: Atrial fibrillation is one of the factors forming thromboembolism. The thromboembolism can be prevented with warfarin. A side effect of warfarin is increasing the occurrence of bleeding, these therapy requires an evaluation of its usage. These evaluations can be seen in the Time in Therapeutic Range (TTR) patients. TTR is the duration in which the International Normalized Ratio (INR) patients in referral value are 2.0-3.0. TTR > 70% can prevent bleeding on FA patients with warfarin therapy.Aim: To determine TTR in patients receiving warfarin with atrial fibrillation.Methods: This study was descriptive with a cross-sectional design for six months at Dr. Kariadi Hospital Semarang. TTR was calculated using Rosendaal's Methods after collecting three INRs and patients' demographics.Results: Total subject was 111 patients with an average age of 52,63 and the TTR average is 53,75. TTR in this study was divided into two groups, that groups were poor TTR (TTR < 70%) with 82 patients (73.88%) and good TTR (≥70%) with 29 patients (26.12%).Conclusions: The average TTR in FA patients with warfarin therapy was 53,75%.
{"title":"TIME IN THERAPEUTIC RANGE (TTR) IN ATRIAL FIBRILLATION WITH WARFARIN THERAPY IN SEMARANG, INDONESIA","authors":"Della Dafina Sekarsari, P. Ardhianto, Erwin Kresnoadi, M. A. Sobirin","doi":"10.14710/DMJ.V10I5.30046","DOIUrl":"https://doi.org/10.14710/DMJ.V10I5.30046","url":null,"abstract":"Background: Atrial fibrillation is one of the factors forming thromboembolism. The thromboembolism can be prevented with warfarin. A side effect of warfarin is increasing the occurrence of bleeding, these therapy requires an evaluation of its usage. These evaluations can be seen in the Time in Therapeutic Range (TTR) patients. TTR is the duration in which the International Normalized Ratio (INR) patients in referral value are 2.0-3.0. TTR > 70% can prevent bleeding on FA patients with warfarin therapy.Aim: To determine TTR in patients receiving warfarin with atrial fibrillation.Methods: This study was descriptive with a cross-sectional design for six months at Dr. Kariadi Hospital Semarang. TTR was calculated using Rosendaal's Methods after collecting three INRs and patients' demographics.Results: Total subject was 111 patients with an average age of 52,63 and the TTR average is 53,75. TTR in this study was divided into two groups, that groups were poor TTR (TTR < 70%) with 82 patients (73.88%) and good TTR (≥70%) with 29 patients (26.12%).Conclusions: The average TTR in FA patients with warfarin therapy was 53,75%. ","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131552675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.14710/DMJ.V10I5.30270
Shobrina Insan Sakina Armunanto, D. P. Dewi, Dodik Pramono, M. Anam
Abstract Background: Asthma is a chronic respiratory disease most often found in children and has the highest prevalence in developing countries. House dust mites are the second most common trigger of asthma whose habitat is influenced by the criteria for a healthy.Objective: Understanding the correlation between sleep density and asthma status in pediatric patients and criteria for a healthy house.Methods: This study was an analytical observational method with a cross sectional study design that took place from June to October 2020. It involved 25 respondents of children with asthma patients aged 3-11 years. There is a confounding variable, namely the criterion score for a healthy house. Dust samples were collected in the bedroom of the respondent diagnosed with bronchial asthma. The identification process using the floating method was carried out at the Parasitology Laboratory, Faculty of Medicine, Diponegoro University, Semarang Indonesia. Respondent data were analyzed using statistical software programs. Non-parametric test with lambda correlation test.Results: The mean of house dust mite’s density in the respondent's house was 3.68 ± 2.32. House dust mite’s density was associated with the criteria for a healthy house (p <0.05), but house dust mite density was not related to the asthma status of pediatric patients (p> 0.05).Conclusion: There is no correlation between pediatric asthma status and house dust mite density.
{"title":"CORRELATION BETWEEN HOUSE DUST MITE DENSITY WITH HEALTHY HOUSE CRITERIA AND ASTHMA STATUS IN PEDIATRIC PATIENTS","authors":"Shobrina Insan Sakina Armunanto, D. P. Dewi, Dodik Pramono, M. Anam","doi":"10.14710/DMJ.V10I5.30270","DOIUrl":"https://doi.org/10.14710/DMJ.V10I5.30270","url":null,"abstract":"Abstract Background: Asthma is a chronic respiratory disease most often found in children and has the highest prevalence in developing countries. House dust mites are the second most common trigger of asthma whose habitat is influenced by the criteria for a healthy.Objective: Understanding the correlation between sleep density and asthma status in pediatric patients and criteria for a healthy house.Methods: This study was an analytical observational method with a cross sectional study design that took place from June to October 2020. It involved 25 respondents of children with asthma patients aged 3-11 years. There is a confounding variable, namely the criterion score for a healthy house. Dust samples were collected in the bedroom of the respondent diagnosed with bronchial asthma. The identification process using the floating method was carried out at the Parasitology Laboratory, Faculty of Medicine, Diponegoro University, Semarang Indonesia. Respondent data were analyzed using statistical software programs. Non-parametric test with lambda correlation test.Results: The mean of house dust mite’s density in the respondent's house was 3.68 ± 2.32. House dust mite’s density was associated with the criteria for a healthy house (p <0.05), but house dust mite density was not related to the asthma status of pediatric patients (p> 0.05).Conclusion: There is no correlation between pediatric asthma status and house dust mite density. ","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"870 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120878644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.14710/DMJ.V10I5.32040
Darmawati Ayu Indraswari, Hafizhil Uzhma Al Ahmadi, Devi Wahyu Arum Sari, T. Jordan, Buwono Puruhito, Edwin Basyar, Saekhol Bakri, M. Muniroh
Background: Overweight and obesity are two health conditions that contribute to the impaired quality of life. Two parameters of obesity also indicating body composition, body mass index (BMI) and waist circumference (WC), have been used as simple tools to assess abdominal visceral fats. The correlation between both measurements and visceral fats remains unclear.Objective: The study aims at demonstrating that body mass index and waist circumference may reflect visceral fats using bioelectrical impedance analysis in adolescents.Methods: First-year students of the Faculty of Medicine Diponegoro University participated in the study. This cross-sectional study measured BMI, WC, mid-arm circumference (MAC), and visceral fat (VF) of 130 participants. BMI, WC, MAC, and VF were assessed using Omron digital scale, measuring tape, and body impedance analysis, respectively. Spearman test was used for the bivariate analysis while multiple regression was employed to perform multivariate analysis. Significant results were determined if p value <0.05 for the bivariates.Results: The correlation between body mass index and visceral fats showed a strong value with r: 0.794 and p-value=<0.001. The correlation between mid-arm circumference and visceral fats showed r= 0.713 and p value=<0.001. Meanwhile the correlation between waist circumference and visceral fats showed r= 0.655 and p value=<0.001. BMI and WC showed the greatest correlation to VF. The comparison between genders resulted in men having stronger relationships between BMI and VF, and WC and VF.Conclusion: BMI and waist circumference are strongly related to visceral fats based on BIA in medical students.
背景:超重和肥胖是导致生活质量受损的两种健康状况。肥胖的两个参数也表明身体组成，身体质量指数(BMI)和腰围(WC)，已被用作评估腹部内脏脂肪的简单工具。这两项测量结果与内脏脂肪之间的相关性尚不清楚。目的:利用生物电阻抗分析方法研究青少年的体重指数和腰围可以反映内脏脂肪。方法:以迪波内戈罗大学医学院一年级学生为研究对象。这项横断面研究测量了130名参与者的BMI、WC、中臂围(MAC)和内脏脂肪(VF)。BMI、WC、MAC和VF分别采用欧姆龙数字秤、卷尺和体阻抗分析进行评估。双因素分析采用Spearman检验，多因素分析采用多元回归。如果双变量的p值<0.05，则确定显著结果。结果:体重指数与内脏脂肪有较强的相关性，r = 0.794, p =<0.001。中臂围与内脏脂肪的相关性r= 0.713, p值<0.001。腰围与内脏脂肪的相关性r= 0.655, p值<0.001。BMI和WC与VF的相关性最大。性别间的比较结果表明，男性BMI与VF、WC与VF的关系更强。结论:基于BIA的医学生BMI和腰围与内脏脂肪有密切关系。
{"title":"Body Mass Index and Waist Circumference are Associated with Visceral Fats Measured by Bioelectrical Impedance Analysis in Adolescents","authors":"Darmawati Ayu Indraswari, Hafizhil Uzhma Al Ahmadi, Devi Wahyu Arum Sari, T. Jordan, Buwono Puruhito, Edwin Basyar, Saekhol Bakri, M. Muniroh","doi":"10.14710/DMJ.V10I5.32040","DOIUrl":"https://doi.org/10.14710/DMJ.V10I5.32040","url":null,"abstract":"Background: Overweight and obesity are two health conditions that contribute to the impaired quality of life. Two parameters of obesity also indicating body composition, body mass index (BMI) and waist circumference (WC), have been used as simple tools to assess abdominal visceral fats. The correlation between both measurements and visceral fats remains unclear.Objective: The study aims at demonstrating that body mass index and waist circumference may reflect visceral fats using bioelectrical impedance analysis in adolescents.Methods: First-year students of the Faculty of Medicine Diponegoro University participated in the study. This cross-sectional study measured BMI, WC, mid-arm circumference (MAC), and visceral fat (VF) of 130 participants. BMI, WC, MAC, and VF were assessed using Omron digital scale, measuring tape, and body impedance analysis, respectively. Spearman test was used for the bivariate analysis while multiple regression was employed to perform multivariate analysis. Significant results were determined if p value <0.05 for the bivariates.Results: The correlation between body mass index and visceral fats showed a strong value with r: 0.794 and p-value=<0.001. The correlation between mid-arm circumference and visceral fats showed r= 0.713 and p value=<0.001. Meanwhile the correlation between waist circumference and visceral fats showed r= 0.655 and p value=<0.001. BMI and WC showed the greatest correlation to VF. The comparison between genders resulted in men having stronger relationships between BMI and VF, and WC and VF.Conclusion: BMI and waist circumference are strongly related to visceral fats based on BIA in medical students. ","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126467876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.14710/dmj.v10i5.30073
Eva Latifatun Ni'mah, D. T. Anantyo, Adhie Nur Radityo Suswihardhyono, Teddy Wahyu Nugroho
Background: Children with low birth weight tend to have a higher risk of experiencing abnormal growth and developmental delays. While the risk of developing obesity in the later years is higher in children with high birth weight. Physical and psychological conditions can affect the quality of life of adolescents.Objective: To analyze the association between birth weight and quality of life in early and middle adolescents.Methods: This cross-sectional study involved 120 students aged 13-16 years in Semarang City in which a consecutive sampling method was used. The study was conducted in August 2020. Research subjects need to fill up the PedsQL, PAQ, birth history, and socio-economic questionnaires. Statistical analyses used were Chi-square and logistic regression tests.Results: Birth weight was significantly associated with quality of life (p=0.002). A history of abnormal birth weight increases the risk of experiencing poor quality of life as a teenager (p=0.002; OR=3.987). Low birth weight was associated with decreased physical function (p=0.001), whereas high birth weight was associated with reduced social and school functioning (p=0.013; 0.032). Gender was significantly associated with quality of life (p=0.016). Girls tend to have a higher risk of experiencing poor quality of life (p=0.021; OR=4.021).Conclusion: Birth weight has a significant association with quality of life in early and middle adolescents. Adolescents with a history of LBW or HBW were more likely to experience decreased quality of life than adolescents with a history of NBW.Keywords: Adolescence, Birth weight, Quality of Life.
{"title":"Association Between Birth Weight and Quality of Life in Early and Middle Adolescence","authors":"Eva Latifatun Ni'mah, D. T. Anantyo, Adhie Nur Radityo Suswihardhyono, Teddy Wahyu Nugroho","doi":"10.14710/dmj.v10i5.30073","DOIUrl":"https://doi.org/10.14710/dmj.v10i5.30073","url":null,"abstract":"Background: Children with low birth weight tend to have a higher risk of experiencing abnormal growth and developmental delays. While the risk of developing obesity in the later years is higher in children with high birth weight. Physical and psychological conditions can affect the quality of life of adolescents.Objective: To analyze the association between birth weight and quality of life in early and middle adolescents.Methods: This cross-sectional study involved 120 students aged 13-16 years in Semarang City in which a consecutive sampling method was used. The study was conducted in August 2020. Research subjects need to fill up the PedsQL, PAQ, birth history, and socio-economic questionnaires. Statistical analyses used were Chi-square and logistic regression tests.Results: Birth weight was significantly associated with quality of life (p=0.002). A history of abnormal birth weight increases the risk of experiencing poor quality of life as a teenager (p=0.002; OR=3.987). Low birth weight was associated with decreased physical function (p=0.001), whereas high birth weight was associated with reduced social and school functioning (p=0.013; 0.032). Gender was significantly associated with quality of life (p=0.016). Girls tend to have a higher risk of experiencing poor quality of life (p=0.021; OR=4.021).Conclusion: Birth weight has a significant association with quality of life in early and middle adolescents. Adolescents with a history of LBW or HBW were more likely to experience decreased quality of life than adolescents with a history of NBW.Keywords: Adolescence, Birth weight, Quality of Life.","PeriodicalId":394284,"journal":{"name":"DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)","volume":"512 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130278978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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