Seeds are usually infected by microorganisms and pests during storage, causing deterioration and reduction in the nutritive and market value of these seeds. In this study, the proximate composition of Vigna unguiculata seeds inoculated with different fungal organisms was determined to ascertain the level of deterioration caused by fungi on the seeds. The fungi used in the study were Botryodiplodia theobromae, Fusarium oxysporum, Rhizopus stolonifer and Aspergillus niger. There was a significant increase (p<0.05, 0.008) in the protein content of seeds inoculated with fungi. Fusarium oxysporum (29.45%) caused the highest increase in protein followed by Aspergillus niger (28.14%), Botryodiplodia theobromae (27.85%) and Rhizopus stolonifer (27.50%). The increase could be attributed to the proteineous content of the fungal mycelia. There was a significant increase (p<0.05, 0.005/0.014) in moisture and ash content of inoculated seeds respectively. Fusarium oxysporum caused the highest increase in ash (7.93) while Rhizopus stolonifer (5.4) caused the lowest increase. The increase in ash content is due to the presence of minerals like potassium and phosphorus in the mycelia of the fungi. There was a significant decrease (p<0.05, 0.019) in the carbohydrate, lipid, fibre and dry matter content of fungi-inoculated seeds when compared with the control. Fusarium oxysporum (36.6) caused the highest decrease while Rhizopus stolonifer (43.2) caused the lowest decrease in dry matter of inoculated seeds. Decrease in dry matter may be as a result of production of enzymes by these fungi.
{"title":"Nutritional changes induced by fungi on cowpea (Vigna unguiculata L. Walp) seeds","authors":"N. G. Iyanyi, A. Ataga","doi":"10.4314/br.v19i1.10","DOIUrl":"https://doi.org/10.4314/br.v19i1.10","url":null,"abstract":"Seeds are usually infected by microorganisms and pests during storage, causing deterioration and reduction in the nutritive and market value of these seeds. In this study, the proximate composition of Vigna unguiculata seeds inoculated with different fungal organisms was determined to ascertain the level of deterioration caused by fungi on the seeds. The fungi used in the study were Botryodiplodia theobromae, Fusarium oxysporum, Rhizopus stolonifer and Aspergillus niger. There was a significant increase (p<0.05, 0.008) in the protein content of seeds inoculated with fungi. Fusarium oxysporum (29.45%) caused the highest increase in protein followed by Aspergillus niger (28.14%), Botryodiplodia theobromae (27.85%) and Rhizopus stolonifer (27.50%). The increase could be attributed to the proteineous content of the fungal mycelia. There was a significant increase (p<0.05, 0.005/0.014) in moisture and ash content of inoculated seeds respectively. Fusarium oxysporum caused the highest increase in ash (7.93) while Rhizopus stolonifer (5.4) caused the lowest increase. The increase in ash content is due to the presence of minerals like potassium and phosphorus in the mycelia of the fungi. There was a significant decrease (p<0.05, 0.019) in the carbohydrate, lipid, fibre and dry matter content of fungi-inoculated seeds when compared with the control. Fusarium oxysporum (36.6) caused the highest decrease while Rhizopus stolonifer (43.2) caused the lowest decrease in dry matter of inoculated seeds. Decrease in dry matter may be as a result of production of enzymes by these fungi.","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90485689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To analyse the extent of disturbances and determine the state of health of aquatic systems, the integration of several biological effects at different levels of biological organization has been used. One of the most valuable methods of biological effect measurements is the use of ecological surveys at community and population levels. In this study, the distributional evaluation of benthic biocoenosis was undertaken to determine site-specific differences in community assemblage in relation to environmental status of the study sites. Three study locations (Okobaba, Iddo and Tin Can Island) in the western side of the Lagos Lagoon with highest concentrations of human activities were selected. These sites represent areas directly affected by major anthropogenic discharge into the Lagos Lagoon. Samples were collected for six consecutive months (March and August 2014). There was great variation in parameters investigated among the study sites. The concentrations of dissolved oxygen in surface water varied from 3.0- 4.5 mg/L. Chlorophyll a in surface water fluctuated from 0.12- 2.01 mg/L whereas, in sediment values varied from 0.14-1.32 mg/g. Biomass of microphytobenthos (MPB) varied from 1.4-13.2 g. Of the total 841 MPB cells collected the highest population was recorded in Okobaba. Twelve MPB taxa were recorded in the study area. The most important species in terms of numerical abundance was Oscillatoria sp. This organism occurred in all the study stations and recorded a total of 113 cells in the study stretch. A major feature of the population distribution of the MPB taxa in this study is the occurrence of higher number of cells at Okobaba. The number of individuals and the distribution of BMF taxa varied greatly from one study location to another. Of the total 801 individuals collected, 398 were recorded at Okobaba, 316 occurred at Tin Can Island and 18 at Iddo. Unlike the case of MPB organisms, a fewer number of BMF taxa were recorded in the study area. The most important MBF taxon in terms of numerical abundance was Pachymelania aurita. This organism occurred in all the study sites and recorded a total of 162 individuals thereby constituting 20% of the total MBF population collected. The benthic community observed in this study was characterized by low number of individuals and the species of MPB and MBF recorded are known opportunistic species common in stressed environments.
{"title":"Stochastic evaluation of benthic biocoenosis as pollution assessment tool in a perturbed aquatic ecosystem","authors":"Roland Efe Uwadie, A. Ajose","doi":"10.4314/br.v19i1.8","DOIUrl":"https://doi.org/10.4314/br.v19i1.8","url":null,"abstract":"To analyse the extent of disturbances and determine the state of health of aquatic systems, the integration of several biological effects at different levels of biological organization has been used. One of the most valuable methods of biological effect measurements is the use of ecological surveys at community and population levels. In this study, the distributional evaluation of benthic biocoenosis was undertaken to determine site-specific differences in community assemblage in relation to environmental status of the study sites. Three study locations (Okobaba, Iddo and Tin Can Island) in the western side of the Lagos Lagoon with highest concentrations of human activities were selected. These sites represent areas directly affected by major anthropogenic discharge into the Lagos Lagoon. Samples were collected for six consecutive months (March and August 2014). There was great variation in parameters investigated among the study sites. The concentrations of dissolved oxygen in surface water varied from 3.0- 4.5 mg/L. Chlorophyll a in surface water fluctuated from 0.12- 2.01 mg/L whereas, in sediment values varied from 0.14-1.32 mg/g. Biomass of microphytobenthos (MPB) varied from 1.4-13.2 g. Of the total 841 MPB cells collected the highest population was recorded in Okobaba. Twelve MPB taxa were recorded in the study area. The most important species in terms of numerical abundance was Oscillatoria sp. This organism occurred in all the study stations and recorded a total of 113 cells in the study stretch. A major feature of the population distribution of the MPB taxa in this study is the occurrence of higher number of cells at Okobaba. The number of individuals and the distribution of BMF taxa varied greatly from one study location to another. Of the total 801 individuals collected, 398 were recorded at Okobaba, 316 occurred at Tin Can Island and 18 at Iddo. Unlike the case of MPB organisms, a fewer number of BMF taxa were recorded in the study area. The most important MBF taxon in terms of numerical abundance was Pachymelania aurita. This organism occurred in all the study sites and recorded a total of 162 individuals thereby constituting 20% of the total MBF population collected. The benthic community observed in this study was characterized by low number of individuals and the species of MPB and MBF recorded are known opportunistic species common in stressed environments.","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84054485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bolajoko Bolarinwa Adewoye, J. Anikwe, W. Makanjuola
This study was conducted to investigate the incidence of rice stem borer infestations and the species composition present in the selected rice fields. Data were collected between April 2017 and November 2018 across three locations, which include the upland rice field in Agbajege, Ogun state, rainfed lowland in Itoikin and Mangrove swamp in Igbogun, Lagos state, respectively. The incidence of rice stem borers was indicated by dead heart and white heads symptoms, while tillers were excised for rice stem borer identification and population. Results showed a high and low incidence of rice stem borers with no significant difference(p>0.05) at p= 0.29 and 0.37 between the early and late planting seasons in the rainfed lowland and upland rice fields respectively. Planting seasons were significant in the incidence of rice stem borers in mangrove swamp rice fields at p<0.05, p=0.01). This study showed that the predominant species of rice stem borers present in the three study sites were Chilo zacconius and Sesamia calamistis. C. zacconius was the most abundant species at 89% while S. calamistis was 11%. Rice stem borer populations were significantly higher at (p<0.05, p=0.000) in flooded zones while the highest rice stem borer populations were observed at the reproductive phase of the rice plants. Thisstudy showed that the planting seasons coincided with the peak period of the development of rice stem borers. Hence, conservation of natural enemies, monitoring and surveillance should be adopted in insect pest management in rice agroecosystems
{"title":"Incidence and relative abundance of rice stem borers in three selected rice fields in Lagos and Ogun States, Nigeria","authors":"Bolajoko Bolarinwa Adewoye, J. Anikwe, W. Makanjuola","doi":"10.4314/br.v19i1.9","DOIUrl":"https://doi.org/10.4314/br.v19i1.9","url":null,"abstract":"This study was conducted to investigate the incidence of rice stem borer infestations and the species composition present in the selected rice fields. Data were collected between April 2017 and November 2018 across three locations, which include the upland rice field in Agbajege, Ogun state, rainfed lowland in Itoikin and Mangrove swamp in Igbogun, Lagos state, respectively. The incidence of rice stem borers was indicated by dead heart and white heads symptoms, while tillers were excised for rice stem borer identification and population. Results showed a high and low incidence of rice stem borers with no significant difference(p>0.05) at p= 0.29 and 0.37 between the early and late planting seasons in the rainfed lowland and upland rice fields respectively. Planting seasons were significant in the incidence of rice stem borers in mangrove swamp rice fields at p<0.05, p=0.01). This study showed that the predominant species of rice stem borers present in the three study sites were Chilo zacconius and Sesamia calamistis. C. zacconius was the most abundant species at 89% while S. calamistis was 11%. Rice stem borer populations were significantly higher at (p<0.05, p=0.000) in flooded zones while the highest rice stem borer populations were observed at the reproductive phase of the rice plants. Thisstudy showed that the planting seasons coincided with the peak period of the development of rice stem borers. Hence, conservation of natural enemies, monitoring and surveillance should be adopted in insect pest management in rice agroecosystems","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88664872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ikenna Bruno Aguh, Zurmi Rabiu Sani, Lynda Chinanu Ohaleme, Andover Alfred Agba
Body mass index (BMI) has traditionally been used as an indicator of body size measure and composition. Although, other measures of adiposity of the abdomen such as waist circumference (WC), waist-hip ratio (WHR), neck circumference (NC) have been suggested as being superior to BMI in predicting disease outcome. This study was designed to compare different anthropometric variables in term of their ability to predict type 2 diabetes mellitus (T2DM). This was a case-control study in 240 participants involving 120 verified T2DM cases and 120 non-diabetics as control. Age, gender and anthropometric data were collected from each participant. Logistic regression models were used with areas under the receiver operating characteristic (AROC) curve to compare the variables predictive statistics. The AROC of WHR to identify T2DM patients was 0.678 (P<0.05), with sensitivity 62.5% of and specificity of 60.8%. The AROC for average arm circumference (AAC) model is 0.649 with sensitivity of 55.8% followed by BMI model (AROC 0.635) and WC model (AROC 0.600) (P<0.05). Hip circumference (HC) (AROC 0.508) and NC (AROC 0.492) models were not significant predictors of T2DM. Subjects of ≥60 years, AAC value ≥32.6 cm, BMI value ≥ 30 kg/m2, and WHR value ≥ 0.93 were at significantly (P<0.05) higher odds of developing T2DM than lower subjects with lower values. There were no significant differences (P>0.05) in the mean HC and NC values between the diabetic and non-diabetic subjects. The non-diabetic subjects have significantly (P>0.05) higher mean height value than the diabetic subjects. Measures of generalized and central obesity were significantly associated with increased risk of developing T2DM. This study revealed that WHR can predict type 2 diabetes mellitus risk more accurately than other anthropometric measures and can thus be helpful in predicting patients with future occurrence of diabetes and providing necessary interventions
{"title":"Anthropometric indices as novel markers of risk in type 2 diabetes mellitus (T2DM) among Nigerian adults in Zamfara State","authors":"Ikenna Bruno Aguh, Zurmi Rabiu Sani, Lynda Chinanu Ohaleme, Andover Alfred Agba","doi":"10.4314/br.v19i1.7","DOIUrl":"https://doi.org/10.4314/br.v19i1.7","url":null,"abstract":"Body mass index (BMI) has traditionally been used as an indicator of body size measure and composition. Although, other measures of adiposity of the abdomen such as waist circumference (WC), waist-hip ratio (WHR), neck circumference (NC) have been suggested as being superior to BMI in predicting disease outcome. This study was designed to compare different anthropometric variables in term of their ability to predict type 2 diabetes mellitus (T2DM). This was a case-control study in 240 participants involving 120 verified T2DM cases and 120 non-diabetics as control. Age, gender and anthropometric data were collected from each participant. Logistic regression models were used with areas under the receiver operating characteristic (AROC) curve to compare the variables predictive statistics. The AROC of WHR to identify T2DM patients was 0.678 (P<0.05), with sensitivity 62.5% of and specificity of 60.8%. The AROC for average arm circumference (AAC) model is 0.649 with sensitivity of 55.8% followed by BMI model (AROC 0.635) and WC model (AROC 0.600) (P<0.05). Hip circumference (HC) (AROC 0.508) and NC (AROC 0.492) models were not significant predictors of T2DM. Subjects of ≥60 years, AAC value ≥32.6 cm, BMI value ≥ 30 kg/m2, and WHR value ≥ 0.93 were at significantly (P<0.05) higher odds of developing T2DM than lower subjects with lower values. There were no significant differences (P>0.05) in the mean HC and NC values between the diabetic and non-diabetic subjects. The non-diabetic subjects have significantly (P>0.05) higher mean height value than the diabetic subjects. Measures of generalized and central obesity were significantly associated with increased risk of developing T2DM. This study revealed that WHR can predict type 2 diabetes mellitus risk more accurately than other anthropometric measures and can thus be helpful in predicting patients with future occurrence of diabetes and providing necessary interventions","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90845986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lawal, B. Olayinka, L. Abdulra’uf, E. O. Etejere
Weed competition has been a major challenge limiting crop yield especially in intercropping systems. Information on the use of chemicals for weed control in intercropping systems appears scanty. The aim of this study was to investigate the efficacy of pendimethalin (P) at 1 kg active ingredient/ha, and hand weeding (HW) on mineral contents of the harvested grains and effects of various weed control treatments in maize, cowpea, and their intercrop. Field experiments were carried out during the 2017 and 2018 rainy seasons. The field layout followed complete randomized block design with three replicates. There were eight treatments: Sole Maize/Cowpea + P, Sole Maize/Cowpea + P + 1HW at 3 weeks after sowing (WAS), Sole Maize/Cowpea + P+ 2HW at 3 and 6 weeks after sowing (WAS), Sole Maize/Cowpea weedy check, Intercrop + P, Intercrop + P + 1HW at 3WAS, Intercrop + P + 2HW at 3 and 6 WAS and Intercrop weedy check. It was observed that, P+1HW and P+2HW in both cropping systems have significant effects (p<0.05) and effectively control weed and increase the mineral contents in maize, cowpea, and their respective intercrop. The study revealed that, the mineral composition of maize and cowpea grains were improved by using pendimethalin with supplementary hand weeding. The study recommends that farmers should adopt intercropping maize with cowpea using pendimethalin plus one supplementary hand weeding at 3 WAS.
{"title":"Influence of pendimethalin and hand weeding on mineral composition of maize, cowpea and their intercrop","authors":"A. Lawal, B. Olayinka, L. Abdulra’uf, E. O. Etejere","doi":"10.4314/BR.V18I2.6","DOIUrl":"https://doi.org/10.4314/BR.V18I2.6","url":null,"abstract":"Weed competition has been a major challenge limiting crop yield especially in intercropping systems. Information on the use of chemicals for weed control in intercropping systems appears scanty. The aim of this study was to investigate the efficacy of pendimethalin (P) at 1 kg active ingredient/ha, and hand weeding (HW) on mineral contents of the harvested grains and effects of various weed control treatments in maize, cowpea, and their intercrop. Field experiments were carried out during the 2017 and 2018 rainy seasons. The field layout followed complete randomized block design with three replicates. There were eight treatments: Sole Maize/Cowpea + P, Sole Maize/Cowpea + P + 1HW at 3 weeks after sowing (WAS), Sole Maize/Cowpea + P+ 2HW at 3 and 6 weeks after sowing (WAS), Sole Maize/Cowpea weedy check, Intercrop + P, Intercrop + P + 1HW at 3WAS, Intercrop + P + 2HW at 3 and 6 WAS and Intercrop weedy check. It was observed that, P+1HW and P+2HW in both cropping systems have significant effects (p<0.05) and effectively control weed and increase the mineral contents in maize, cowpea, and their respective intercrop. The study revealed that, the mineral composition of maize and cowpea grains were improved by using pendimethalin with supplementary hand weeding. The study recommends that farmers should adopt intercropping maize with cowpea using pendimethalin plus one supplementary hand weeding at 3 WAS.","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86948109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elodie Gbotche, V. Dougnon, Y. Chabi, Sosthéne Vissoh, J. Agbankpe, E. Déguénon, P. Sédah, K. Fabiyi, A. Missihoun, L. Baba-Moussa, H. Bankolé, C. Agbangla
Antimicrobial resistance is a real public health problem. All over the world, it has a considerable impact in hospitals. The present study was conducted to ascertain the bacterial ecology in two hospitals in Benin as well as the resistance genes present in the recovered isolates. A total of 146 environmental and catheter samples were collected at the University Hospital Center of Abomey-Calavi / So-Ava and at the Beninese Army Hospital of Cotonou. These samples were inoculated on Mannitol Salt and Eosin Methylene Blue agars. The colonies obtained were identified and their sensitivity to antibiotics were tested, using the Kirby Bauer technique. Four resistance genes encoding the production of extended-spectrum beta-lactamases (blaCTX-M1, blaCTX-M2, blaCTX-M9, blaCTX-M15) and the gene coding for methicillin resistance (mecA) were screened. The gene coding for methicillin resistance (mecA) was sought in staphylococci. A total of 69 (53,49%) and 60 (46,51%) strains belonging to Enterobacteriaceae family and staphylococci were identified, respectively. A predominance of Staphylococcus aureus (25.6%) followed by Enterobacter cloacae (21.0%) and coagulase negative staphylococci (21.0%) was observed. These bacterial strains showed multidrug-resistance, particularly to beta-lactams, fluoroquinolones, aminoglycosides, and macrolides. Beta-lactamases were identified in the genome of bacterial strains with a predominance of blaCTX-M15 (42.8%). The frequency of the mecA gene in staphylococci was 50%. These results show the magnitude of the antimicrobial resistance situation in the hospitals investigated. They can be used to support advocacy for urgent action at the national level, especially with regards to the management and efficient use of antimicrobials in Benin.
{"title":"Molecular characterization of Enterobacteriaceae producing β-lactamase and methicillin-resistant staphylococci isolated from the hospital environment and catheters in two public hospitals in Benin, Republic of Benin","authors":"Elodie Gbotche, V. Dougnon, Y. Chabi, Sosthéne Vissoh, J. Agbankpe, E. Déguénon, P. Sédah, K. Fabiyi, A. Missihoun, L. Baba-Moussa, H. Bankolé, C. Agbangla","doi":"10.4314/br.v18i2.5","DOIUrl":"https://doi.org/10.4314/br.v18i2.5","url":null,"abstract":"Antimicrobial resistance is a real public health problem. All over the world, it has a considerable impact in hospitals. The present study was conducted to ascertain the bacterial ecology in two hospitals in Benin as well as the resistance genes present in the recovered isolates. A total of 146 environmental and catheter samples were collected at the University Hospital Center of Abomey-Calavi / So-Ava and at the Beninese Army Hospital of Cotonou. These samples were inoculated on Mannitol Salt and Eosin Methylene Blue agars. The colonies obtained were identified and their sensitivity to antibiotics were tested, using the Kirby Bauer technique. Four resistance genes encoding the production of extended-spectrum beta-lactamases (blaCTX-M1, blaCTX-M2, blaCTX-M9, blaCTX-M15) and the gene coding for methicillin resistance (mecA) were screened. The gene coding for methicillin resistance (mecA) was sought in staphylococci. A total of 69 (53,49%) and 60 (46,51%) strains belonging to Enterobacteriaceae family and staphylococci were identified, respectively. A predominance of Staphylococcus aureus (25.6%) followed by Enterobacter cloacae (21.0%) and coagulase negative staphylococci (21.0%) was observed. These bacterial strains showed multidrug-resistance, particularly to beta-lactams, fluoroquinolones, aminoglycosides, and macrolides. Beta-lactamases were identified in the genome of bacterial strains with a predominance of blaCTX-M15 (42.8%). The frequency of the mecA gene in staphylococci was 50%. These results show the magnitude of the antimicrobial resistance situation in the hospitals investigated. They can be used to support advocacy for urgent action at the national level, especially with regards to the management and efficient use of antimicrobials in Benin.","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86679409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Parkia biglobosa (Jacq) is a wild leguminous plant found in North-Central zone of Nigeria with high calorific value, essential proteins, fatty acids, and vitamins. The study investigated the antimicrobial activity of crude extracts of fermented and unfermented P. biglobosa seeds on selected clinical microbial isolates namely, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. P. biglobosa seeds were obtained from Oja-Oba market in Ilorin, Kwara State, Nigeria. The samples were pre-treated and pulverized into powder. The extraction was achieved with acetone and water and qualitative phytochemical analysis was performed following standard procedures. The antimicrobial activity of the extracts against the isolates was determined by agar well diffusion method. Qualitative phytochemical screening of the crude extracts showed the presence of tannins, alkaloid, flavonoid, saponin and glycosides. P. aeruginosa was sensitive to the aqueous extract of fermented seeds having a zone of inhibition of 14.00±1.00mm while for unfermented seeds it was 10.00±2.00 mm at 100 mg/ml. The acetone extracts of both fermented and unfermented seeds revealed antibacterial activity against P. aeruginosa with zone of inhibition of 17.00±3.00 mm and 18.00±0.00 mm respectively. In conclusion, the crude extracts of the fermented and unfermented P. biglobosa seeds at a concentration of 75 and 100 mg/ml respectively have antimicrobial effect on the clinical isolates.
{"title":"Antimicrobial activity of the crude extracts of Parkia biglobosa (Jacq) seeds on selected clinical isolates","authors":"A. Ajiboye, B. Hammed","doi":"10.4314/br.v18i2.2","DOIUrl":"https://doi.org/10.4314/br.v18i2.2","url":null,"abstract":"Parkia biglobosa (Jacq) is a wild leguminous plant found in North-Central zone of Nigeria with high calorific value, essential proteins, fatty acids, and vitamins. The study investigated the antimicrobial activity of crude extracts of fermented and unfermented P. biglobosa seeds on selected clinical microbial isolates namely, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. P. biglobosa seeds were obtained from Oja-Oba market in Ilorin, Kwara State, Nigeria. The samples were pre-treated and pulverized into powder. The extraction was achieved with acetone and water and qualitative phytochemical analysis was performed following standard procedures. The antimicrobial activity of the extracts against the isolates was determined by agar well diffusion method. Qualitative phytochemical screening of the crude extracts showed the presence of tannins, alkaloid, flavonoid, saponin and glycosides. P. aeruginosa was sensitive to the aqueous extract of fermented seeds having a zone of inhibition of 14.00±1.00mm while for unfermented seeds it was 10.00±2.00 mm at 100 mg/ml. The acetone extracts of both fermented and unfermented seeds revealed antibacterial activity against P. aeruginosa with zone of inhibition of 17.00±3.00 mm and 18.00±0.00 mm respectively. In conclusion, the crude extracts of the fermented and unfermented P. biglobosa seeds at a concentration of 75 and 100 mg/ml respectively have antimicrobial effect on the clinical isolates. \u0000 ","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85164346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
UF Babaiwa, S. Eraga, EO Ojugo, O. Erharuyi, J. Akerele
The study investigated the antimicrobial properties and the chemical composition of ethyl acetate extract of Dennettia tripetala (pepper fruit) seeds. Crude extract obtained by maceration of pulverized seeds in ethyl acetate was evaluated for antimicrobial activity against Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger using standard agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extract was oily, yellowish-brown with a yield of 1.66 % and had activity against most of the test microorganisms, with inhibition zone diameters ranging between 10 to 25 mm. About 41 chemical constituents were present in the extract with formic acid methyl esters and fatty acids accounting for 57.23 and 18.49 % respectively. Ethyl acetate extract of Dennettia tripetala seeds possessed antimicrobial activity against bacteria but not fungi. The observed activity may be due to the presence of formic and fatty acid esters in the seed. The study further established a scientific proof for the traditional use of Dennettia tripetala seed extracts in treating microbial infections.
{"title":"A preliminary study on the antimicrobial activities and gas chromatography-mass spectrometry (GC-MS) analysis of the ethyl acetate extract of Dennettia tripetala G. Baker seeds","authors":"UF Babaiwa, S. Eraga, EO Ojugo, O. Erharuyi, J. Akerele","doi":"10.4314/br.v18i1.4","DOIUrl":"https://doi.org/10.4314/br.v18i1.4","url":null,"abstract":"The study investigated the antimicrobial properties and the chemical composition of ethyl acetate extract of Dennettia tripetala (pepper fruit) seeds. Crude extract obtained by maceration of pulverized seeds in ethyl acetate was evaluated for antimicrobial activity against Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger using standard agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extract was oily, yellowish-brown with a yield of 1.66 % and had activity against most of the test microorganisms, with inhibition zone diameters ranging between 10 to 25 mm. About 41 chemical constituents were present in the extract with formic acid methyl esters and fatty acids accounting for 57.23 and 18.49 % respectively. Ethyl acetate extract of Dennettia tripetala seeds possessed antimicrobial activity against bacteria but not fungi. The observed activity may be due to the presence of formic and fatty acid esters in the seed. The study further established a scientific proof for the traditional use of Dennettia tripetala seed extracts in treating microbial infections. \u0000 ","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88463622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Candida is currently the most implicated pathogenic fungal species recognized as the major cause of a variety of human infections all over the world. This study investigated species distribution, enzymatic activities, and antifungal resistance profiles of human and animal Candida species. Clinical Candida species (n=220) were isolated from urine, high vaginal swab (HVS) and blood while Candida species (n=128) were isolated from rectal swab, ear swab, blood, feces, and milk in animals: goat, sheep, cattle, pig and chicken. The identification of the species was performed using standard methods. Enzymatic activity was screened using plate methods. Susceptibility testing was carried out using disk diffusion and broth microdilution methods. A statistically significant difference (P=0.031) was observed in the distribution of Candida spp. recovered from humans and animals. The Pz values of human Candida species for proteinase, hemolysin, lipase and phospholipase were 0.65±0.97, 0.61±0.81, 0.59±0.47 and 0.76±0.74 respectively while that of Candida species recovered from animal were 0.67±0.13, 0.61±0.95, 0.62±0.67 and 0.69±0.70 respectively. No statistically significant difference (P>0.05) in the in vitro enzymatic activity was observed between the two groups. High azole-resistance rate was observed. Resistance was higher among human Candida isolates compared to animal isolates although the difference was not considered statistically significant (p = 0.519). Our findings suggest that the enzymatic activity (virulence potential) and resistance patterns are similar in the two groups investigated. This study underscores the importance of animals especially pets and their products as potential sources/reservoirs of pathogenic and multi-azole resistant Candida species in Nigeria.
{"title":"Antifungal resistance profile and enzymatic activity of Candida species recovered from human and animal samples","authors":"IE Mba, E. Nweze","doi":"10.4314/br.v17i1.4","DOIUrl":"https://doi.org/10.4314/br.v17i1.4","url":null,"abstract":"Candida is currently the most implicated pathogenic fungal species recognized as the major cause of a variety of human infections all over the world. This study investigated species distribution, enzymatic activities, and antifungal resistance profiles of human and animal Candida species. Clinical Candida species (n=220) were isolated from urine, high vaginal swab (HVS) and blood while Candida species (n=128) were isolated from rectal swab, ear swab, blood, feces, and milk in animals: goat, sheep, cattle, pig and chicken. The identification of the species was performed using standard methods. Enzymatic activity was screened using plate methods. Susceptibility testing was carried out using disk diffusion and broth microdilution methods. A statistically significant difference (P=0.031) was observed in the distribution of Candida spp. recovered from humans and animals. The Pz values of human Candida species for proteinase, hemolysin, lipase and phospholipase were 0.65±0.97, 0.61±0.81, 0.59±0.47 and 0.76±0.74 respectively while that of Candida species recovered from animal were 0.67±0.13, 0.61±0.95, 0.62±0.67 and 0.69±0.70 respectively. No statistically significant difference (P>0.05) in the in vitro enzymatic activity was observed between the two groups. High azole-resistance rate was observed. Resistance was higher among human Candida isolates compared to animal isolates although the difference was not considered statistically significant (p = 0.519). Our findings suggest that the enzymatic activity (virulence potential) and resistance patterns are similar in the two groups investigated. This study underscores the importance of animals especially pets and their products as potential sources/reservoirs of pathogenic and multi-azole resistant Candida species in Nigeria.","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88582889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. C. Ikegbunam, E. Osayi, R. Njokuocha, O. J. Walter
The diversity and abundance of airborne palynomorphs and other bio particles in the atmosphere of Obukpa was studied for a period of three months, from June through August 2010. The palynomorphs were trapped with Tauber sampler mounted at three locations during the period of study. A total of three thousand, eight hundred and ninety-nine (3,899) palynomorphs consisting of 3365 (86.3%) pollen grains, 496 (12.72%) fungal spores and 38 (0.98%) of other bio particles (insect parts and trichomes) was identified from the study. The pollen types which dominated the atmosphere of the sampled area included members of Combretaceae/Melastomataceae, Poaceae, Asteraceae, Amarathaceae, Lannea sp., Pentaclethra macrophylla and Alchornea cordifolia . The identified pollen grains were representatives of some trees, shrubs, herbs and grasses. The major contributors to the fugal aerospora were Glomerularia sp., Nigrospora sp., Cercospora sp., Asperosporium sp., Fusoma , Dreschlera , Helminthosporium and so on. Comparatively, there were variations in the quantity of the trapped palynomorphs during the three months study period which could have resulted from differences in the flowering period of the plants as well as influence of some meteorological factors such as rainfall, temperature, wind speed, relative humidity and wind direction. Keywords: Palynomorphs, Pollen types, bio particles, fungal spores, atmosphere
{"title":"Aeropalynological study of Obukpa town, Enugu State, Nigeria","authors":"N. C. Ikegbunam, E. Osayi, R. Njokuocha, O. J. Walter","doi":"10.4314/br.v17i1.1","DOIUrl":"https://doi.org/10.4314/br.v17i1.1","url":null,"abstract":"The diversity and abundance of airborne palynomorphs and other bio particles in the atmosphere of Obukpa was studied for a period of three months, from June through August 2010. The palynomorphs were trapped with Tauber sampler mounted at three locations during the period of study. A total of three thousand, eight hundred and ninety-nine (3,899) palynomorphs consisting of 3365 (86.3%) pollen grains, 496 (12.72%) fungal spores and 38 (0.98%) of other bio particles (insect parts and trichomes) was identified from the study. The pollen types which dominated the atmosphere of the sampled area included members of Combretaceae/Melastomataceae, Poaceae, Asteraceae, Amarathaceae, Lannea sp., Pentaclethra macrophylla and Alchornea cordifolia . The identified pollen grains were representatives of some trees, shrubs, herbs and grasses. The major contributors to the fugal aerospora were Glomerularia sp., Nigrospora sp., Cercospora sp., Asperosporium sp., Fusoma , Dreschlera , Helminthosporium and so on. Comparatively, there were variations in the quantity of the trapped palynomorphs during the three months study period which could have resulted from differences in the flowering period of the plants as well as influence of some meteorological factors such as rainfall, temperature, wind speed, relative humidity and wind direction. Keywords: Palynomorphs, Pollen types, bio particles, fungal spores, atmosphere","PeriodicalId":39601,"journal":{"name":"Annals of Agri Bio Research","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87038561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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