Pub Date : 2021-04-01DOI: 10.21608/EJOH.2020.53855.1157
A. Ezzat, H. Ahmed, S. Osman, S. H. G. El-Hak
Yardlong bean (Vigna unguiculata (L.) Walp. sub. sp. sesquipedalis) is a climber annual Vigna crop of Leguminosae family with chromosome number 2n = 2x = 22. It’s known as long-podded cowpea, asparagus bean, pea bean, snake bean, garter bean and Chinese long bean. It has ability to fix atmospheric nitrogen with its symbiotic bacteria (Rhizobia) and increases productivity of the soil (Pandey et al., 1989). It is much more a climbing and trailing plant than the cowpea, often reaching 9-12 feet in height and it is characterized by its very long pods 30-90 cm in length with seeds usually 8–12 mm long. It is commonly cultivated T study was carried out to identify the genetic variations in yardlong bean plants irradiated by various doses (75, 150, 300, 450 and 600Gy) of gamma rays using RAPD and ISSR markers techniques as well as, their effect on some vegetative traits were studied. ten RAPD and 10 ISSR markers were used in this investigation. The obtained results showed that the ten RAPD primers produced 117 bands only 94 of them were polymorphic while 23 bands were monomorphic. The percentages of polymorphism among primers ranged from 0.0% to 100%. All of the RAPD primers produced unique bands except OPK-06 primer which did not produced any unique bands. Meanwhile, 62 amplified bands including 26 polymorphic and 36 monomorphic bands were generated by ten ISSR primers. All of the ISSR primers did not produce any unique bands except ISSR4 primer which produced only one unique band, these results demonstrated that this unique sequence could be used as a molecular marker associated with gamma irradiation. The field results showed that the emergence percentage decreased with increasing doses up to 600Gy treatment which gave the lowest emergence percentage. Inconstant values of vegetative characteristics were obtained after each treatment with gamma ray doses; 300Gy treatment gave the highest value of plant height, number of branches, pod length and number of seeds per pod compared to control and other treatments. The values of coefficients of phenotypic and genotypic variation, heritability and expect genetic advance did not follow regular increase or decrease with the radiation doses in both seasons. The present data demonstrate that using gamma rays to induce DNA polymorphism in this plant genome which might be lead to appearance of a desired phenotype and genetic characteristics which could be used in yardlong bean improvement programs.
{"title":"Molecular and Morphological Variations Induced by Gamma Rays in Yardlong Bean","authors":"A. Ezzat, H. Ahmed, S. Osman, S. H. G. El-Hak","doi":"10.21608/EJOH.2020.53855.1157","DOIUrl":"https://doi.org/10.21608/EJOH.2020.53855.1157","url":null,"abstract":"Yardlong bean (Vigna unguiculata (L.) Walp. sub. sp. sesquipedalis) is a climber annual Vigna crop of Leguminosae family with chromosome number 2n = 2x = 22. It’s known as long-podded cowpea, asparagus bean, pea bean, snake bean, garter bean and Chinese long bean. It has ability to fix atmospheric nitrogen with its symbiotic bacteria (Rhizobia) and increases productivity of the soil (Pandey et al., 1989). It is much more a climbing and trailing plant than the cowpea, often reaching 9-12 feet in height and it is characterized by its very long pods 30-90 cm in length with seeds usually 8–12 mm long. It is commonly cultivated T study was carried out to identify the genetic variations in yardlong bean plants irradiated by various doses (75, 150, 300, 450 and 600Gy) of gamma rays using RAPD and ISSR markers techniques as well as, their effect on some vegetative traits were studied. ten RAPD and 10 ISSR markers were used in this investigation. The obtained results showed that the ten RAPD primers produced 117 bands only 94 of them were polymorphic while 23 bands were monomorphic. The percentages of polymorphism among primers ranged from 0.0% to 100%. All of the RAPD primers produced unique bands except OPK-06 primer which did not produced any unique bands. Meanwhile, 62 amplified bands including 26 polymorphic and 36 monomorphic bands were generated by ten ISSR primers. All of the ISSR primers did not produce any unique bands except ISSR4 primer which produced only one unique band, these results demonstrated that this unique sequence could be used as a molecular marker associated with gamma irradiation. The field results showed that the emergence percentage decreased with increasing doses up to 600Gy treatment which gave the lowest emergence percentage. Inconstant values of vegetative characteristics were obtained after each treatment with gamma ray doses; 300Gy treatment gave the highest value of plant height, number of branches, pod length and number of seeds per pod compared to control and other treatments. The values of coefficients of phenotypic and genotypic variation, heritability and expect genetic advance did not follow regular increase or decrease with the radiation doses in both seasons. The present data demonstrate that using gamma rays to induce DNA polymorphism in this plant genome which might be lead to appearance of a desired phenotype and genetic characteristics which could be used in yardlong bean improvement programs.","PeriodicalId":40934,"journal":{"name":"EGYPTIAN JOURNAL OF VETERINARY SCIENCE","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49136532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-01DOI: 10.21608/EJOH.2021.57711.1163
Rasha I. Anwar, N. Abdel-Hamid, S. Nasr, A. R. Rahman
Citrus is one of the most important fruit crops grown under tropical and subtropical conditions in many countries. At this moment, there is about 3.6 million fed. of citrus spp. cultivated at commercial scale in the world yielding nearly 40 million metric tons of oranges, lemons, limes,... etc. In Egypt, citrus has great attention due to its importance for local consumption and consider a popular fruit for it’s low acidity, juicy pulp and good flavor. All over, citrus fruits have an important role to prevent human cardiovascular diseases, and source for foreign currencies by exportation to the world markets. Recently, citrus cultivated area has increased rapidly with the reclamation of new desert lands reaching about486650 fed. And Valencia orange occupied 139851 fed. (28.74 % of total area) (Ministry of Agriculture and Land Reclamation, 2018). Fruit harvested through March to June period. In Egypt many Citriculture regions suffer from a low organic matter and high alkaline with limited phosphorus availability which causes, many problems are encountered that threatening some citrus growers and been among the difficulties hindering more extension. Generally, alkaline soil is a serious problem especially in arid or semi-arid regions. Irrigated water pH value is an important feature of water. Predecessors have reported the effects of different pH treatments on plant growth in Metroxylon sagu (Anugoolprasert et al., 2012), Camellia sinensis (Ruan et al., 2007), Chlamydomonas acidophila (Gerloff-Elias et al., 2005), Anabaenopsis elenkini (Santos et al., 2011) VALENCIA orange (Citrus sinensis) seedlings on Sour orange (Citrus aurantum ) “ SO” or Volkamer lemon (Citrus Volkamariana) “ VL” fertilized with three nutrient doses 50 ; 75 and 100 percentage of nursery recommendation and irrigated with water at four pH levels : the control ; pH5 ; pH7 and pH9. .Results showed that; Valencia orange seedlings on both SO or VL stocks gave the best stem: height and diameter; leaf area; root length and width; under 50% or 75 % nutrient doses of nursery recommendation when irrigated with water at pH7. SO stock fertilized with 50% nutrient dose and irrigated with water at pH7 gave the highest leaf Chl. a content , also, VL stock under 50% or 75% nutrient dose and irrigated with water at pH7 gave the same trend. Whereas, both stocks under 50% or 75% dose and irrigated with water at pH7 gave the highest leaf Chl. b content. SO or VL stocks fertilized with 50% or 75% of the control and irrigated with water pH7 significantly gave the highest leaf carbohydrates contents, both citrus stocks fertilized with 100% the control and irrigated with water pH9 was the lowest. SO or VL stocks fertilized with50% or 75% doses and irrigated with water pH7 significantly increased N , P & K percentage, but which fertilized with 100% dose and irrigated with water pH7 or pH9 was the lowest.
柑橘是许多国家在热带和亚热带条件下种植的最重要的水果作物之一。目前，世界上大约有360万种柑橘类作物以商业规模种植，产量接近4000万吨橙子、柠檬、酸橙等。等。在埃及，柑橘因其对当地消费的重要性而受到极大关注，并因其低酸度、多汁的果肉和美味而被认为是一种受欢迎的水果。在世界各地，柑橘类水果在预防人类心血管疾病方面具有重要作用，并通过出口到世界市场获得外汇。近年来，柑橘种植面积迅速增加，新开垦荒漠土地约486650公顷，巴伦西亚橙占地139851公顷(占总面积的28.74%)(农垦部，2018)。在三月到六月期间收获的水果。在埃及，许多柑橘种植区遭受低有机质和高碱性的困扰，磷利用率有限，这导致遇到许多问题，威胁到一些柑橘种植者，并成为阻碍进一步推广的困难之一。一般来说，碱性土壤是一个严重的问题，特别是在干旱或半干旱地区。灌溉水的pH值是水的一个重要特征。前人已经报道了不同pH处理对沙棉(Anugoolprasert et al.， 2012)、山茶(Ruan et al.， 2007)、嗜酸衣藻(Gerloff-Elias et al.， 2005)、elenkini Anabaenopsis elenkini (Santos et al.， 2011)瓦伦夏橙(Citrus sinensis)幼苗在三种营养剂量50的酸橙(Citrus aurantum)“SO”或Volkamer柠檬(Citrus Volkamariana)“VL”上的生长的影响;75%和100%的苗圃推荐，用四种pH值的水灌溉:对照;pH5;pH7和pH9.结果表明;瓦伦西亚橙幼苗在SO和VL砧木上的茎高和茎粗最好;叶面积;根长、根宽;当用pH7的水灌溉时，苗圃推荐营养剂量低于50%或75%。施50%养分量和pH7水灌溉的苏木叶片Chl最高。50%或75%营养剂量下的VL砧木和pH7水灌溉的VL砧木也有相同的变化趋势。而50%和75%剂量和pH7水灌溉的砧木叶片Chl最高。b的内容。施用50%或75%对照并以pH7灌溉的SO和VL砧木叶片碳水化合物含量最高，施用100%对照和pH9灌溉的柑橘砧木叶片碳水化合物含量最低。50%和75%施肥和pH7水灌溉显著提高了土壤N、P、K百分比，而100%施肥和pH7、pH9水灌溉的土壤N、P、K百分比最低。
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Pub Date : 2021-04-01DOI: 10.21608/EJOH.2021.55847.1161
Eltohamy A. A. Yousef, I. Nasef
Garlic (Allium sativum L.) is one of the oldest and most economic vegetables. It has been demonstrated that garlic was grown and consumed by ancient Egyptian, about 2780 2100 B.C., (Yamaguchi, 1983). Egypt is one the world’s top garlic producing countries, it is ranked as the fourth country among garlic producers, with an annual production of 286,213 tones produced from 127,82 ha (FAO, 2018). In Egypt, garlic is grown mainly for local consumption, however Egypt is one of the top ten garlic exporter countries with 0.9% of total worldwide garlic exportations with a net return of 27 million dollars. Garlic cloves have several bioactive compounds, such as allicin, alliin, diallyl sulfide, diallyl disulfide, diallyl trisulfide, ajoene, and S-allyl-cysteine, which contribute to the garlic’s taste (Kilgori et al., 2007, Shang et al., 2019). Owing to its diverse and valuable compounds, several earlier studies have been reported that garlic and its bioactive ingredients can positively contribute to human health as anti-oxidant, anti-inflammatory, anti-microbial, anti-cancer, anti-diabetic and anti-obesity (Shang et al., 2019). In addition, garlic’s oil has several agriculture actions, such as herbicide, acaricide and insecticide (Abouziena et al., 2009, Ismail et al., 2011, Sharaby et al., 2012). Furthermore, it has a stimulatory effect on onion plants grown under sandy soil conditions T successive field trials were performed at the Agricultural Research Farm of the Faculty of Agriculture, Suez Canal University during the two successive of 2018/2019 and 2019/2020. to investigate the influence of 0, 0.5 and 1 mM of indole 3-butyric acid (IBA) and 0, 1, 1.5 and 2 mM myo-inositol (MI) and their interactive as folair application on vegetative growth, bulb yield, biochemical constituents and mineral contents of garlic plants cv. Seds 40. The treatments were laid out in a split plot order with three replicates. The obtained results reported that garlic plants positively responded to the exogenous treatments of IBA and MI individually or interactively. It showed generally that application of IBA at 0.5 mM recorded the highest values of vegetative growth, yiled, quality parmemters as well as the mineral content (N, P and K). In the same regard, application of MI at 1.5 and 2 mM significantly promoted moast of studied traits compared to control and the rest of MI concentration, in both growing seasons. Furthermore, the two-factor interaction was found to be significant for all measured traits in both seasons, except for K content. Whereas, exogenous treatment of IBA at 0.5 mM along with exogenous treatment of MI at 1.5 and/or 2 mM resulted in the highest means of most of the above mentioned traits. Thus, it could be suggested that applying a combination of IBA and MI was found to be effective for enhancing vegetative growth, bulb yield and quality as well as macro-elements content.
大蒜（Allium sativum L.）是最古老、最经济的蔬菜之一。据证明，大蒜是古埃及人种植和食用的，大约在公元前2780年至2100年（Yamaguchi，1983）。埃及是世界上最大的大蒜生产国之一，在大蒜生产国中排名第四，年产量为286213吨，产量为127.82公顷（粮农组织，2018）。在埃及，大蒜主要用于当地消费，但埃及是十大大蒜出口国之一，占全球大蒜出口总额的0.9%，净回报为2700万美元。蒜瓣含有几种生物活性化合物，如大蒜素、大蒜素，二烯丙基硫化物、二烯丙基二硫化物、二烯基三硫化物、阿焦烯和S-烯丙基半胱氨酸，它们有助于大蒜的味道（Kilgori等人，2007，Shang等人，2019）。由于其多样且有价值的化合物，几项早期研究报告称，大蒜及其生物活性成分可以作为抗氧化、抗炎、抗菌、抗癌、抗糖尿病和抗肥胖等对人类健康做出积极贡献（Shang et al.，2019）。此外，大蒜油具有多种农业作用，如除草剂、杀螨剂和杀虫剂（Abouziena等人，2009年，Ismail等人，2011年，Sharaby等人，2012年）。此外，它对在沙质土壤条件下生长的洋葱植物有刺激作用。2018/2019年和2019/2020年连续两次在苏伊士运河大学农业学院农业研究农场进行了连续的田间试验。研究0、0.5和1mM吲哚-3-丁酸（IBA）和0、1、1.5和2mM肌醇（MI）及其相互作用对Seds 40大蒜植株营养生长、鳞茎产量、生化成分和矿物质含量的影响。处理按三个重复的分割图顺序排列。结果表明，大蒜植株对IBA和MI的外源处理分别或相互作用都有积极的反应。结果表明，施用0.5mM IBA的营养生长、产量、质量指标以及矿物质含量（N、P和K）最高。同样，在两个生长季节，与对照和MI浓度的其余部分相比，施用1.5和2mM的MI显著促进了所研究性状的生长。此外，除了K含量外，两个因素的相互作用在两个季节的所有测量性状中都是显著的。然而，0.5mM的IBA的外源处理以及1.5和/或2mM的MI的外源处理导致了上述大多数性状的最高平均值。因此，IBA和MI联合应用对提高营养生长、鳞茎产量和品质以及常量元素含量是有效的。
{"title":"Exogenous Application Effect of Indole 3-Butyric Acid and Myo-inositol on Improving Growth, Productivity and Bulb Quality of Garlic","authors":"Eltohamy A. A. Yousef, I. Nasef","doi":"10.21608/EJOH.2021.55847.1161","DOIUrl":"https://doi.org/10.21608/EJOH.2021.55847.1161","url":null,"abstract":"Garlic (Allium sativum L.) is one of the oldest and most economic vegetables. It has been demonstrated that garlic was grown and consumed by ancient Egyptian, about 2780 2100 B.C., (Yamaguchi, 1983). Egypt is one the world’s top garlic producing countries, it is ranked as the fourth country among garlic producers, with an annual production of 286,213 tones produced from 127,82 ha (FAO, 2018). In Egypt, garlic is grown mainly for local consumption, however Egypt is one of the top ten garlic exporter countries with 0.9% of total worldwide garlic exportations with a net return of 27 million dollars. Garlic cloves have several bioactive compounds, such as allicin, alliin, diallyl sulfide, diallyl disulfide, diallyl trisulfide, ajoene, and S-allyl-cysteine, which contribute to the garlic’s taste (Kilgori et al., 2007, Shang et al., 2019). Owing to its diverse and valuable compounds, several earlier studies have been reported that garlic and its bioactive ingredients can positively contribute to human health as anti-oxidant, anti-inflammatory, anti-microbial, anti-cancer, anti-diabetic and anti-obesity (Shang et al., 2019). In addition, garlic’s oil has several agriculture actions, such as herbicide, acaricide and insecticide (Abouziena et al., 2009, Ismail et al., 2011, Sharaby et al., 2012). Furthermore, it has a stimulatory effect on onion plants grown under sandy soil conditions T successive field trials were performed at the Agricultural Research Farm of the Faculty of Agriculture, Suez Canal University during the two successive of 2018/2019 and 2019/2020. to investigate the influence of 0, 0.5 and 1 mM of indole 3-butyric acid (IBA) and 0, 1, 1.5 and 2 mM myo-inositol (MI) and their interactive as folair application on vegetative growth, bulb yield, biochemical constituents and mineral contents of garlic plants cv. Seds 40. The treatments were laid out in a split plot order with three replicates. The obtained results reported that garlic plants positively responded to the exogenous treatments of IBA and MI individually or interactively. It showed generally that application of IBA at 0.5 mM recorded the highest values of vegetative growth, yiled, quality parmemters as well as the mineral content (N, P and K). In the same regard, application of MI at 1.5 and 2 mM significantly promoted moast of studied traits compared to control and the rest of MI concentration, in both growing seasons. Furthermore, the two-factor interaction was found to be significant for all measured traits in both seasons, except for K content. Whereas, exogenous treatment of IBA at 0.5 mM along with exogenous treatment of MI at 1.5 and/or 2 mM resulted in the highest means of most of the above mentioned traits. Thus, it could be suggested that applying a combination of IBA and MI was found to be effective for enhancing vegetative growth, bulb yield and quality as well as macro-elements content.","PeriodicalId":40934,"journal":{"name":"EGYPTIAN JOURNAL OF VETERINARY SCIENCE","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47086849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.21608/ejoh.2020.41337.1142
G. Yücel, K. Erken, Y. Doğan
Synthetic plant growth regulators have widely been used for many years. However, restrictions are imposed in traditional agriculture today due to the harms of certain agricultural practices to natural resources and the environment (Bhargavi et al., 2018). The new policies and restrictions of the European Union on environmental protection and pesticides urge member countries to limit, change and remove active ingredients of synthetic growth preparations (Khan et al., 2009, Pacholczak et al., 2013 and Pacholczak et al., 2017). Substances that are effective in plant development are also supposed to be not harmful to the environment according to the limitations (Pacholczak et al., 2012a).
合成植物生长调节剂已被广泛应用多年。然而，由于某些农业做法对自然资源和环境的危害，今天对传统农业施加了限制(Bhargavi等人，2018)。欧盟在环保和农药方面的新政策和限制促使成员国限制、改变和去除合成生长制剂的有效成分(Khan et al.， 2009; Pacholczak et al.， 2013; Pacholczak et al.， 2017)。根据限制，对植物发育有效的物质也应该对环境无害(Pacholczak et al.， 2012a)。
{"title":"Organic Stimulant Uses In Natural Plant Production","authors":"G. Yücel, K. Erken, Y. Doğan","doi":"10.21608/ejoh.2020.41337.1142","DOIUrl":"https://doi.org/10.21608/ejoh.2020.41337.1142","url":null,"abstract":"Synthetic plant growth regulators have widely been used for many years. However, restrictions are imposed in traditional agriculture today due to the harms of certain agricultural practices to natural resources and the environment (Bhargavi et al., 2018). The new policies and restrictions of the European Union on environmental protection and pesticides urge member countries to limit, change and remove active ingredients of synthetic growth preparations (Khan et al., 2009, Pacholczak et al., 2013 and Pacholczak et al., 2017). Substances that are effective in plant development are also supposed to be not harmful to the environment according to the limitations (Pacholczak et al., 2012a).","PeriodicalId":40934,"journal":{"name":"EGYPTIAN JOURNAL OF VETERINARY SCIENCE","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43897105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.21608/ejoh.2020.47863.1151
Safaa A. Mohamed, Hatem Ramadan, Mostafa Elabd
{"title":"Improve Fruit Quality and Yield of Plum cv. “African Rose” By Different Thinning Treatments","authors":"Safaa A. Mohamed, Hatem Ramadan, Mostafa Elabd","doi":"10.21608/ejoh.2020.47863.1151","DOIUrl":"https://doi.org/10.21608/ejoh.2020.47863.1151","url":null,"abstract":"","PeriodicalId":40934,"journal":{"name":"EGYPTIAN JOURNAL OF VETERINARY SCIENCE","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49132199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-01DOI: 10.21608/EJOH.2020.46354.1150
M. F. Abdalla, M. Soliman,, Ayman Mrtwally, Mohamed Dokishy
A FIELD experiment was conducted to study the response of vegetative growth and fruit yield of eggplant (Solanum melongena L.) cv Classic Roomy and chili pepper (Capsicum annuum L.) cv Omega F1 hybrid transplants produced using different growing media. The growing media were the spent mushroom compost and peat moss, in addition to their 1,1 (v/v mixture. The study was conducted in the Vegetable Research Station, Faculty of Agriculture, Assiut University, Assiut during 2016 and 2017. The 40-day-old eggplant and chili pepper transplants were transferred to the open field and arranged as randomized complete-block design. Data recorded on some main growth and yield traits showed no influence of growing media on field productivity of pepper plants. Likewise was the eggplant, except a marginal total yield difference that was in favor of transplants grown in the spent mushroom compost growing media. The increment in fruit weight for eggplant was noticed in one year only for those plants derived from transplants grown in the spent mushroom compost growing medium. These results suggests the feasibility of spent mushroom compost substitution to imported peat moss as growing media for production of eggplant and pepper seedlings and may also be used for other horticultural species.
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