Pub Date : 2022-12-27DOI: 10.21608/puj.2022.174048.1193
A. Elkholy, Nagat H. Soliman, M. Elawady, R. Omar
Background: Vaccination against schistosomiasis might be an excellent approach for its control and elimination. Despite the recent advances in identification of several potential vaccine candidates, no vaccine was validated for clinical trials. Objective: This research aims to evaluate the preventive effects of soluble egg antigen (SEA) in combination with schistosomula lung antigen preparation (SLAP) on a murine model of schistosomiasis mansoni . Material and Methods: In this study, 35 Swiss albino male mice (aged 6 to 8 weeks) were divided into five groups. Each group contained seven mice, with G1 serving as the normal control, G2 the infected control, G3 the infected and immunized by SEA, G4 the infected and immunized by SLAP, and G5 the infected and immunized by combination antigens (SLAP and SEA). All mice were infected two weeks after receiving their final dose of immunization. Seven weeks following infection, mice were decapitated in order to determine the impact of the injected antigens using determination of liver enzymes, IL10, TNF-α and schistosomal
{"title":"Immunological evaluation of the prophylactic impact of soluble egg antigen (SEA) combined with schistosomula lung antigen preparation (SLAP) on a murine model of schistosomiasis mansoni","authors":"A. Elkholy, Nagat H. Soliman, M. Elawady, R. Omar","doi":"10.21608/puj.2022.174048.1193","DOIUrl":"https://doi.org/10.21608/puj.2022.174048.1193","url":null,"abstract":"Background: Vaccination against schistosomiasis might be an excellent approach for its control and elimination. Despite the recent advances in identification of several potential vaccine candidates, no vaccine was validated for clinical trials. Objective: This research aims to evaluate the preventive effects of soluble egg antigen (SEA) in combination with schistosomula lung antigen preparation (SLAP) on a murine model of schistosomiasis mansoni . Material and Methods: In this study, 35 Swiss albino male mice (aged 6 to 8 weeks) were divided into five groups. Each group contained seven mice, with G1 serving as the normal control, G2 the infected control, G3 the infected and immunized by SEA, G4 the infected and immunized by SLAP, and G5 the infected and immunized by combination antigens (SLAP and SEA). All mice were infected two weeks after receiving their final dose of immunization. Seven weeks following infection, mice were decapitated in order to determine the impact of the injected antigens using determination of liver enzymes, IL10, TNF-α and schistosomal","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49086461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-27DOI: 10.21608/puj.2022.171955.1192
S. Abaza
{"title":"Recent advances in identification of potential drug targets and development of novel drugs in protozoal diseases. Part IV. Apicomplexans and Plasmodium spp.","authors":"S. Abaza","doi":"10.21608/puj.2022.171955.1192","DOIUrl":"https://doi.org/10.21608/puj.2022.171955.1192","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48306322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-27DOI: 10.21608/puj.2022.177647.1197
Radwa G Diab, Salwa S Younis
vaccine candidates identified against schistosomiasis are protein molecules expressed on the tegument surface or secreted by schistosomula such as
针对血吸虫病鉴定的候选疫苗是在被膜表面表达或由血吸虫分泌的蛋白质分子，例如
{"title":"Omics: Applications related to diagnosis, treatment, prevention and control of parasitic diseases. Part II. Helminths.","authors":"Radwa G Diab, Salwa S Younis","doi":"10.21608/puj.2022.177647.1197","DOIUrl":"https://doi.org/10.21608/puj.2022.177647.1197","url":null,"abstract":"vaccine candidates identified against schistosomiasis are protein molecules expressed on the tegument surface or secreted by schistosomula such as","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49455274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-24DOI: 10.21608/puj.2022.169218.1190
D. Said, Hoda M Khalifa, Lamia El-Samad, M. Meheissen, Hala E. Diab, Radwa G Diab
Background: Maggot debridement therapy is a therapeutic wound myiasis that depends mainly on L. sericata larva. Owing to its availability and cheap breeding, M. domestica was suggested as an alternative to Lucilia . Objective: The present study was designed to assess the role of M. domestica larvae-derived substances on wound healing in immunocompetent and immunosuppressed mice. Material and Methods: Chitosan and gut extracts, obtained from M. domestica larvae, were applied daily on skin wounds of Swiss albino mice. Mice were divided into two groups, control non-treated mice (I), and experimental treated mice (II). Each group included immunocompetent (a), and immunosuppressed (b) mice. The experimental subgroups were treated with either chitosan (1) or gut extracts (2). Wounds were assessed by macroscopic evaluation, wound contracture, histopathological, immunohistochemical and bacteriological parameters. Results: In control non treated mice hair growth was evident with normal underlying skin by the end of the experiment (4 weeks) except in immunosuppressed (Ib) subgroup. Significant reduction in wound size was detected on the 7 th day post wounding (PW) in the immunocompetent-chitosan-treated subgroup
{"title":"THE EFFECTS OF COMMON HOUSE FLY (MUSCA DOMESTICA) LARVAE-DERIVED SUBSTANCES ON WOUND HEALING IN MOUSE MODEL","authors":"D. Said, Hoda M Khalifa, Lamia El-Samad, M. Meheissen, Hala E. Diab, Radwa G Diab","doi":"10.21608/puj.2022.169218.1190","DOIUrl":"https://doi.org/10.21608/puj.2022.169218.1190","url":null,"abstract":"Background: Maggot debridement therapy is a therapeutic wound myiasis that depends mainly on L. sericata larva. Owing to its availability and cheap breeding, M. domestica was suggested as an alternative to Lucilia . Objective: The present study was designed to assess the role of M. domestica larvae-derived substances on wound healing in immunocompetent and immunosuppressed mice. Material and Methods: Chitosan and gut extracts, obtained from M. domestica larvae, were applied daily on skin wounds of Swiss albino mice. Mice were divided into two groups, control non-treated mice (I), and experimental treated mice (II). Each group included immunocompetent (a), and immunosuppressed (b) mice. The experimental subgroups were treated with either chitosan (1) or gut extracts (2). Wounds were assessed by macroscopic evaluation, wound contracture, histopathological, immunohistochemical and bacteriological parameters. Results: In control non treated mice hair growth was evident with normal underlying skin by the end of the experiment (4 weeks) except in immunosuppressed (Ib) subgroup. Significant reduction in wound size was detected on the 7 th day post wounding (PW) in the immunocompetent-chitosan-treated subgroup","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44512365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.21608/puj.2022.174437.1196
R. Mohamed, A. Darwish, M. Mohamed
{"title":"Hormonal changes in Trypanosoma evansi-infected Rattus norvegicus: An approach to understand host-parasite interaction","authors":"R. Mohamed, A. Darwish, M. Mohamed","doi":"10.21608/puj.2022.174437.1196","DOIUrl":"https://doi.org/10.21608/puj.2022.174437.1196","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47557306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-29DOI: 10.21608/puj.2022.156355.1185
Aliaa M Elsawey, S. Etewa, N. Aboulfotouh
{"title":"Stem cells therapy research for the treatment of parasitic infections","authors":"Aliaa M Elsawey, S. Etewa, N. Aboulfotouh","doi":"10.21608/puj.2022.156355.1185","DOIUrl":"https://doi.org/10.21608/puj.2022.156355.1185","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45357853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-22DOI: 10.21608/puj.2022.155256.1182
Samar Habib
Background: The immune response against L. donovani depends significantly on infected macrophages. Since Leishmania amastigotes deploy several immune suppressive mechanisms to escape host immune responses, macrophages polarize towards the classically activated macrophages (M1) or the alternatively activated macrophages (M2). The balance between both types is crucial in shaping the infection outcome. Objective: The aim of this study is to explore the macrophage polarization behavior in response to L. donovani infection, and to examine the differential expression of IL-10 and TNF-α by each phenotype. Material and Methods: Leishmania -infected phorbol 12-myristate 13-acetate (PMA)-treated human leukemia monocytic cell line (THP-1) was used as an in vitro model of Leishmania infection. Leishmania stationary phase promastigotes were used to infect the macrophages at different multiplicities of infections (MOIs) i.e., ratio of macrophages to stationary phase promastigotes at 1:1, 1:10, and 1:20; and time points of 24 and 48 h post infection (PI). While CD68, CD40, HLA-DR were used as markers for M1; CD68 and CD163 were used to characterize
{"title":"Leishmania donovani promotes macrophages polarization towards M2 phenotype in vitro: A new approach to identify a new therapeutic target","authors":"Samar Habib","doi":"10.21608/puj.2022.155256.1182","DOIUrl":"https://doi.org/10.21608/puj.2022.155256.1182","url":null,"abstract":"Background: The immune response against L. donovani depends significantly on infected macrophages. Since Leishmania amastigotes deploy several immune suppressive mechanisms to escape host immune responses, macrophages polarize towards the classically activated macrophages (M1) or the alternatively activated macrophages (M2). The balance between both types is crucial in shaping the infection outcome. Objective: The aim of this study is to explore the macrophage polarization behavior in response to L. donovani infection, and to examine the differential expression of IL-10 and TNF-α by each phenotype. Material and Methods: Leishmania -infected phorbol 12-myristate 13-acetate (PMA)-treated human leukemia monocytic cell line (THP-1) was used as an in vitro model of Leishmania infection. Leishmania stationary phase promastigotes were used to infect the macrophages at different multiplicities of infections (MOIs) i.e., ratio of macrophages to stationary phase promastigotes at 1:1, 1:10, and 1:20; and time points of 24 and 48 h post infection (PI). While CD68, CD40, HLA-DR were used as markers for M1; CD68 and CD163 were used to characterize","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45547894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.21608/puj.2022.156229.1184
Hebat-Allah S. A. Yousof, M. Khater, S. El-Sayed
{"title":"Spotlights on helminth pathogens’ innovative pathways of Tumorigenesis","authors":"Hebat-Allah S. A. Yousof, M. Khater, S. El-Sayed","doi":"10.21608/puj.2022.156229.1184","DOIUrl":"https://doi.org/10.21608/puj.2022.156229.1184","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68554144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-19DOI: 10.21608/puj.2022.159135.1186
Fatima Zahran, R. Meselhey
{"title":"Evaluation of the effect of an aromatase inhibitor drug \"Letrozole\" and an estrogen receptor modulator \"Tamoxifen\" on Toxoplasma gondii tachyzoites in-vitro","authors":"Fatima Zahran, R. Meselhey","doi":"10.21608/puj.2022.159135.1186","DOIUrl":"https://doi.org/10.21608/puj.2022.159135.1186","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41700967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-09DOI: 10.21608/puj.2022.162366.1189
S. Abaza
{"title":"Spotlights on recent publications: Novel drug targets XX","authors":"S. Abaza","doi":"10.21608/puj.2022.162366.1189","DOIUrl":"https://doi.org/10.21608/puj.2022.162366.1189","url":null,"abstract":"","PeriodicalId":41408,"journal":{"name":"Parasitologists United Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42272362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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