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Bazı Organik Besin Kaynaklarının Cin Mısırın (Zea mays L. everta) Tane Verimine Etkisi
IF 0.6 Pub Date : 2018-04-05 DOI: 10.15832/ANKUTBD.446383
H. Cihangir, A. Öktem
This study was carried out to determine effect of sixteen different organic nutrition sources to grain yield of popcorn (Zea mays L. everta) in Cermik district of Diyarbakir province of Turkey between 2010 and 2011 years. The research was established according to Randomized Complete Block Designs (RCBD) with 3 replicates and Ant-Cin 98 popcorn variety was used as crop material in the study. Organic nutrition sources were torf, compost, cattle manure, chicken manure, horse manure, sheep manure, pigeon manure, vermicompost, seaweed + cattle manure, compost + humic acid, cattle manure + humic acid, chicken manure + humic acid, horse manure + humic acid, sheep manure + humic acid and torf + humic acid except conventional. In both years, applications affected significantly popcorn yield (P≤0.01). According to average of two years, the highest popcorn grain yields were found at seaweed + cattle manure, horse manure + humic acid and sheep manure + humic acid applications. Grain yields of these applications were 526.54 kg da-1, 516.85 kg da-1 and 497.07 kg da-1, respectively. Seaweed + cattle manure, horse manure + humic acid, sheep manure + humic acid and pigeon manure of applications were caused a 9.47%, 7.45%, 3.34%, 0.52% increase in yield compared with the conventional application, respectively. Also economic analysis was performed in this study. The greatest net profits were obtained from horse manure + humic acid application (2280.64 TL da-1) in 2010 while horse manure (2545.82 TL da-1) application in 2011. According to yield, quality and economic net profit; horse manure, chicken manure, compost, cattle manure, sheep manure and humic acid applications can be used in organic popcorn farming.
在2010 ~ 2011年间,研究了16种不同有机营养源对土耳其迪亚巴克尔省切尔米克地区玉米籽粒产量的影响。试验采用随机完全区组设计(RCBD),设3个重复,以抗霉素98玉米品种为作物材料。有机营养源除常规营养源外依次为:土豆泥、堆肥、牛粪、鸡粪、马粪、羊粪、鸽粪、蚯蚓堆肥、海藻+牛粪、堆肥+腐植酸、牛粪+腐植酸、鸡粪+腐植酸、马粪+腐植酸、羊粪+腐植酸和土豆泥+腐植酸。在这两个年份,施用对玉米产量影响显著(P≤0.01)。按两年平均计算,海草+牛粪、马粪+腐植酸和羊粪+腐植酸施用的爆米花粒产量最高。产量分别为526.54 kg、516.85 kg、497.07 kg da-1。海藻+牛粪、马粪+腐植酸、羊粪+腐植酸和鸽粪分别比常规施用增产9.47%、7.45%、3.34%和0.52%。并进行了经济分析。2010年施用马粪+腐植酸获得净利润最多(2280.64 TL da-1), 2011年施用马粪获得净利润最多(2545.82 TL da-1)。根据产量、质量和经济净利润;马粪、鸡粪、堆肥、牛粪、羊粪和腐植酸都可以用于有机爆米花种植。
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引用次数: 2
An Evaluation of the Performance of Forced Air Cooling on Cooling Parameters in Transient Heat Transfer at Different Layers of Pomegranate 强制空气冷却对石榴不同层间瞬态换热冷却参数的影响
IF 0.6 Pub Date : 2018-04-05 DOI: 10.15832/ankutbd.446362
M. Behaeen, A. Mahmoudi, S. Ranjbar
The quality of horticultural products can be promoted using high techniques. One of these methods is precooling applied before storage and leads to increased shelf and storage life of the fruit. For this reason, the effect of forced air cooling was conducted to investigate the cooling rate at the center (aril), spongy tissue (peel) and leathery skin (rind) of pomegranate (Punica granatum L.). Airflow velocity as an effective factor in cooling products at three levels of 0.5, 1, and 1.3 m s-1 and temperature of 7.2 °C was considered. Cooling parameters including lag factor and cooling coefficient were calculated from experimental data. Then, half-cooling time and seven-eighths cooling time were obtained at different layers of pomegranate. Cooling heterogeneity was analyzed at different air velocity and at different layers of pomegranate. The results showed that increase in air velocity from 0.5 to 1.3 m s-1, reduced the half-cooling time and seven-eighths cooling time. After 5000 seconds, the change of air velocity had a slight influence on decreasing temperature of different layers of pomegranate. Cooling heterogeneity at the air velocity of 0.5 m s-1 was low and then increased at the air velocity of 1 m s-1. Finally, at the air velocity of 1.3 m s-1, it was declined. The overall results illustrate that the applied methodology in this research, which explains unsteady heat transfer in the cooling process, can be performed in pomegranate or similarly shaped fruits.
利用高新技术可以提高园艺产品的质量。其中一种方法是在储存前进行预冷,从而增加水果的货架和储存寿命。为此,对石榴(Punica granatum L.)中部(假种皮)、海绵状组织(果皮)和革质皮(皮)的冷却速率进行了研究。在0.5、1和1.3 m s-1三个水平和7.2°C温度下,风速是冷却产品的有效因素。根据实验数据计算出滞后系数和冷却系数等冷却参数。然后,得到石榴不同层数的半冷却时间和八分之七冷却时间。分析了石榴在不同风速和不同层数下的冷却不均一性。结果表明,风速由0.5 m s-1增加到1.3 m s-1,可缩短半冷却时间和7 / 8冷却时间。5000秒后,风速的变化对石榴各层的降温影响不大。冷却非均匀性在0.5 m s-1风速下较低,在1 m s-1风速下增大。最后,在风速为1.3 m s-1时,它有所下降。总体结果表明,本研究中解释冷却过程中不稳定传热的应用方法可以在石榴或类似形状的水果中执行。
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引用次数: 0
Effects of Heat Stress after Anthesis on PSII Photochemical Efficiency and the Antioxidant Activity of Wheat Cultivars 花后热胁迫对小麦品种PSII光化学效率及抗氧化活性的影响
IF 0.6 Pub Date : 2018-04-05 DOI: 10.15832/ankutbd.446391
Mahroo Mojtabaie Zmani, M. Nabipour, M. Meskarbashee
This study was conducted to investigate the effects of heat stress after anthesis on the performance of Photosystem II (PSII) and the enzymatic activity of catalase and ascorbate peroxidase. Two treatments-normal and heat stress-were conducted on four bread wheat cultivars from 7 days after anthesis until maturity. Heat stress led to an acceleration of chlorosis, reduced the efficiency of electron transfer and increased concentrations of malondialdehyde; however, the level of susceptibility varied depending on the cultivars. On the 10th day of heat stress, reductions in fluorescence parameters, depending on the cultivar, were 6.9 to 18.9% for Fv/Fm, 9 to 21% for ΦPSII and 8.3 to 19.4% for F’v/F’m compared to normal conditions. Catalase activity increased after initial exposure to heat stress. However, after 10 days of treatment, catalase activity increased in the Chamran and Aflak cultivars by 32% and 45%, respectively, but it did not change in the Dez cultivar and decreased 22% in Darab2. Ascorbate peroxidase activity decreased in two treatments, while the amount of reduction in heat stress treatment was more than the normal treatment. The highest levels of enzymatic activity were observed in Chamran under heat stress conditions, whereas Darab2 and Dez showed the lowest activity of the enzymes. Chlorophyll fluorescence parameters and chlorophyll index had a significant negative correlation with the levels of malondialdehyde; however, they had a significant positive correlation with the antioxidant activity.
本试验旨在研究开花后热胁迫对苜蓿光系统II (PSII)性能及过氧化氢酶和抗坏血酸过氧化物酶活性的影响。对4个面包小麦品种从开花后7 d至成熟进行了正常胁迫和热胁迫处理。热应激导致黄化加速,电子传递效率降低,丙二醛浓度增加;然而,不同品种的敏感性不同。在热胁迫第10天,不同品种的荧光参数与正常条件相比,Fv/Fm降低了6.9 ~ 18.9%,ΦPSII降低了9 ~ 21%,F’v/F’m降低了8.3 ~ 19.4%。过氧化氢酶活性在初始暴露于热应激后增加。然而,处理10天后,Chamran和Aflak品种过氧化氢酶活性分别提高了32%和45%,而Dez品种没有变化,Darab2下降了22%。抗坏血酸过氧化物酶活性在两个处理中均有所下降,但热应激处理的下降幅度大于正常处理。热胁迫条件下Chamran的酶活性最高,而Darab2和Dez的酶活性最低。叶绿素荧光参数和叶绿素指数与丙二醛水平呈显著负相关;然而,它们与抗氧化活性呈显著正相关。
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引用次数: 2
Jasmonic Acid Induced Systemic Resistance in Infected Cucumber by Pythium aphanidermatum 茉莉酸诱导黄瓜被蛇皮霉侵染后的全身抗性
IF 0.6 Pub Date : 2018-04-05 DOI: 10.15832/ANKUTBD.446416
E. Sabbagh, S. Sabbagh, N. Panjehkeh, Hamid Reza Bolok-Yazdi
Damping-off disease of cucumber is one of the most destructive diseases of cucumber in worldwide. In this work, the potential of jasmonic acid (JA) for induce resistant against damping off disease was investigated. The effect of JA on activity of Polyphenol oxidase (PPO), Peroxidase (PO) and Catalase (CAT) enzymes and total phenol was assayed by spectrophotometric method. Expression level of three plant defense genes as Lipoxygenase, Cupi4 and Phenylalanine ammonia-lyase genes was analyzed using qRT-PCR method. Drop-plate method was used to assay inhibitory effect of JA on radial growth of fungi. Exogenic application of JA decreased disease severity in the infected plants but did not inhibit mycelia growth on solid medium compared to control. Our results showed that JA application substantially increased the activity of oxidative enzymes at different concentration. The highest enzyme activity was recorded after 48 hours post infection (hpi) at a concentration of 400 mg L-1 of JA. Gene expression analysis revealed that JA is differentially able to increase the mRNA transcripts of all tested genes at 48 hpi. The expression level of Cupi4 gene was higher than the other genes in treated plants. Induced systemic resistance by JA was mediated through an enhanced expression of ISR marker genes and increase of antioxidant enzymes activity. Based on these results, we suggest that exogenic application of JA could be considered as plant resistance inducer.
黄瓜萎蔫病是世界上最具破坏性的黄瓜病害之一。本文研究了茉莉酸(jasmonic acid, JA)诱导抗减湿病的潜力。用分光光度法测定了茉莉酸对植物多酚氧化酶(PPO)、过氧化物酶(PO)和过氧化氢酶(CAT)活性及总酚的影响。采用qRT-PCR方法分析了脂氧合酶、Cupi4和苯丙氨酸解氨酶3个植物防御基因的表达水平。采用滴平板法测定茉莉酸对真菌径向生长的抑制作用。与对照相比,外源施用JA降低了侵染植株的病害严重程度,但未抑制菌丝在固体培养基上的生长。结果表明,施用JA可显著提高不同浓度下的氧化酶活性。当JA浓度为400 mg L-1时,感染后48小时酶活性最高。基因表达分析显示,在48 hpi时,JA能够显著增加所有测试基因的mRNA转录。Cupi4基因在处理植株中的表达量高于其他基因。茉莉酸诱导的全身抗性是通过增加ISR标记基因的表达和增加抗氧化酶的活性来介导的。基于这些结果,我们认为JA可以作为植物抗性诱导剂外源应用。
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引用次数: 13
Developing a Machine Vision System to Detect Weeds from Potato Plant 马铃薯杂草的机器视觉检测系统研究
IF 0.6 Pub Date : 2018-04-05 DOI: 10.15832/ANKUTBD.446402
S. Sabzi, Y. A. Gilandeh, H. Javadikia
crops, different weeds grow along with potatoes in agricultural fields. These weeds reduce the performance of crops due to competing with them to absorb water, light, and nutrients from soil. Accordingly, in this study, a machine vision system with the hybrid artificial neural network-ant colony algorithm (ANN-ACO) classifier was developed for a site-specific spraying considering the weed type. Potato plant and three weed types including Chenopodium album, Polygonum aviculare L., and Secale cereale L. were used in this study. A digital camera (SAMSUNG WB151F (CCD, 14.2 MP, 30f/s) was placed in the center of the video acquisition system. The distance between plants and the digital camera was fixed at 40 cm. For video acquisition, only lamps of white LED with a light intensity of 327 lux were selected. For filming in order to evaluate the proposed system, a 4-hectare area of Agria potato fields in Kermanshah-Iran (longitude: 7.03°E; latitude: 4.22°N) was selected. Employing the Gamma test, among 31 features, 5 features (Luminance and Hue corresponding to YIQ color space, Autocorrelation, Contrast, and Correlation) were selected. The correct classification accuracy for testing and training data using three classifiers of the hybrid ANN-ACO, radial basis function (RBF) artificial neural network, and Discriminant analysis (DA) was 99.6% and 98.13%, 97.24% and 91.23%, and 69.8% and 70.8%, respectively. The results show that the accuracy of DA statistical method is much lower than that of the hybrid ANN-ACO classifier. Consequently, the results of the present study can be used in machine vision system for the optimum spraying of herbicides.
农作物,不同的杂草和土豆一起生长在农田里。这些杂草由于与它们竞争从土壤中吸收水分、光和养分而降低了作物的产量。基于此,本研究基于混合人工神经网络-蚁群算法(ANN-ACO)分类器,开发了一种针对杂草类型的定点喷洒机器视觉系统。以马铃薯植物为研究对象,以藜、蓼、禾鳞3种杂草为研究对象。在视频采集系统的中央放置一台数码相机(三星WB151F, CCD, 14.2 MP, 30f/s)。植物与数码相机之间的距离固定为40厘米。视频采集只选择光强为327勒克斯的白光LED灯。为了评估拟议的系统,在Kermanshah-Iran的一个4公顷的Agria马铃薯田(经度:7.03°E;纬度:4.22°N)。采用Gamma检验,从31个特征中选择5个特征(YIQ色彩空间对应的Luminance和Hue、Autocorrelation、Contrast和Correlation)。混合ANN-ACO、径向基函数(RBF)人工神经网络和判别分析(DA)三种分类器对测试和训练数据的正确分类准确率分别为99.6%和98.13%、97.24%和91.23%、69.8%和70.8%。结果表明,DA统计方法的准确率远低于ANN-ACO混合分类器。因此,本研究结果可用于机器视觉系统中除草剂的最佳喷洒。
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引用次数: 6
Effect of Environment and Genotype on the Protein Quality Attributes and Baking Characteristic of Newly Developed Wheat Inbred Lines 环境和基因型对小麦自交系蛋白质品质属性和烘烤特性的影响
IF 0.6 Pub Date : 2018-04-05 DOI: 10.15832/ankutbd.446407
N. A. Mutwali, A. I. Mustafa, I. M. Ahmed, E. E. Babiker
The present work examined the effect of genotype and environment on protein content and fractions, gluten and starch fraction, SSL (sodium stearoyl-2-lactylate) and DMG (distilled mono glyceride) binding ability of starch and specific loaf volume (SLV) of six wheat genotypes grown in three different environment. Genotype and environment significantly affected all quality attributes under investigation. However, protein content and fractions showed differences in relative effects of genotype and environment. Most of the protein quality characteristics were influenced more by genotype than environment. Size distribution of gluten subunits was significantly affected by genotype and environment. It was observed that as the flour protein content increased, the magnitudes of monomeric proteins appeared to rise, but glutenin decreased. Flour protein content was expressively associated with gliadin and dough making characteristics. Environment influenced b eedings.
研究了基因型和环境对6个基因型小麦在3种不同生长环境下蛋白质含量和组分、面筋和淀粉组分、硬脂酰-2-乳酸钠(SSL)和蒸馏单甘油酯(DMG)淀粉结合能力和比面包体积(SLV)的影响。基因型和环境对所有品质性状均有显著影响。蛋白质含量和组分对基因型和环境的相对影响存在差异。大部分蛋白质品质性状受基因型的影响大于环境的影响。谷蛋白亚基的大小分布受基因型和环境的显著影响。结果表明,随着面粉中蛋白质含量的增加,单体蛋白含量呈上升趋势,而谷蛋白含量呈下降趋势。面粉蛋白含量与麦胶蛋白和面团特性有显著的相关性。环境影响幼苗生长。
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引用次数: 1
Hook Selectivity for Bluefish (Pomatomus saltatrix Linneaus, 1766) in Gallipoli Peninsula and Çanakkale Strait (Northern Aegean Sea, Turkey) 加利波利半岛和Çanakkale海峡(土耳其爱琴海北部)蓝鱼钩选择性(Pomatomus saltatrix Linneaus, 1766)
IF 0.6 Pub Date : 2018-04-05 DOI: 10.15832/ANKUTBD.446380
Alkan Öztekin, A. Ayaz, Uğur Özekinci, Can Ali Kumova
This study was conducted to determine the selectivity of the hooks used for bluefish (Pomatomus saltatrix Linneaus, 1766) in the Gallipoli Peninsula and the Dardanelles between 2006 and 2009 fishing seasons (November to September). Bluefish were fished with hooks sized 1, 1/0, 2/0, 3/0, 4/0 and in sum; 1210 bluefish were caught. The hook no 2/0 caught the highest number of fish (344 fish, 20.43%) and the hook no 1 caught the least (35 fish, 2.89%). Length frequency distribution of bluefish, which were caught with different hook sizes, was used in SELECT method and according to the results; the normal scale model gave the best fit for selectivity. The normal scale model was used to calculate model length (ML) and spread value (SV) of each hook size. Model length and spread value were found as follows; 19.18 cm ML and 4.44 SV for hook no. 1; 21.88 cm ML, 5.07 SV for hook no 1/0; 24.14 cm ML, 5.59 SV for hook no. 2/0; 27.02 cm ML, 6.26 SV for hook no. 3/0; 28.19 cm ML, 6.53 SV for hook no. 4/0, respectively. Because the minimum landing size (MLS) for bluefish has been stipulated as 20.0 cm (TL) in the Turkish Fishery Regulations, the use of hook no. 2/0 or bigger hook sizes can be recommended for fishing of bluefish.
本研究旨在确定2006年至2009年捕鱼季节(11月至9月)加利波利半岛和达达尼尔海峡蓝鱼(Pomatomus saltatrix Linneaus, 1766)所用鱼钩的选择性。蓝鱼的鱼钩尺寸分别为1、1/0、2/0、3/0、4/0和总和;捕获了1210条蓝鱼。2/0号鱼钩捕获鱼最多(344条,20.43%),1号鱼钩捕获鱼最少(35条,2.89%)。采用SELECT方法,根据不同鱼钩大小捕获的蓝鱼的长度频率分布;标准比例模型对选择性的拟合效果最好。采用正比例模型计算各鱼钩尺寸的模型长度(ML)和扩散值(SV)。模型长度和扩散值如下:钩号为19.18 cm ML, 4.44 SV。1;21.88 cm ML, 1/0号钩5.07 SV;24.14 cm ML,钩号5.59 SV。2/0;27.02 cm ML, 6.26 SV,钩号。3/0;28.19 cm ML,钩号6.53 SV。分别为4/0。由于《土耳其渔业条例》规定蓝鱼的最小上岸尺寸(MLS)为20.0厘米(TL),因此使用钩号。钓蓝鱼时,建议使用2/0或更大的鱼钩。
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引用次数: 7
PCR Based Approach for Detection of Bovine Babesiosis in Suspected Carrier Cattle and Vector Ticks in Sri Lanka 斯里兰卡疑似携带牛和媒介蜱巴贝斯虫病的PCR检测方法
IF 0.6 Pub Date : 2018-01-01 DOI: 10.4038/JAS.V13I1.8300
R. Vimonish, G. Galhena, M. Magamage
Purpose: Cattle recovered from clinical babesiosis become carriers for a certain period posing a threat of transmitting the disease to the entire herd. Diagnosis of carrier cattle is important for preventing outbreaks of babesiosis. The objective of the present study therefore, was to establish a sensitive rapid detection method for bovine babesiosis in suspected carrier cattle using nested PCR (nPCR). Research Method: Accordingly, 30 blood samples and ticks were collected from suspected carrier cattle representing two bio-climatic zones of Sri Lanka. Blood samples were analysed by both microscopically and nested nPCR methods for detection of bovine babesiosis. Further ticks were analysed for morphological idenfication using micrscopy and for babesiosis with nPCR. Findings: 47% (14/30) among the investigated samples became positive for the babesia infection with light microscopy, while nPCR analysis diagnosed 90% (27/30) as positive. This indicates that, 43% (13/30) of the animals which appeared to be healthy through routine light microscopical diagnosis were in fact carriers posing a major threat to the  healthy herd. Further, according to the results of nPCR, 22% (6/27) of the blood samples were positive only for Babesia bovis infection, 11% (3/27) only for Babesia bigemina infection and 67% (18/27) for mixed infection with both parasites. The dominant tick vector isolated from both zones was Rhiphicephalus microplus. Out of the examined ticks, 21% (5/24) were positive for Babesia bigemina infection. Research limitations: Since the  sample size from all sites of both climatic zones were uneven and small, data could not be analysed for statistical significance. Originality/Value: However, the results from this study indicate that nPCR provides a sensitive screening method to detect bovine babesiosis compared to the conventional microscopic analysis.
目的:从临床巴贝斯虫病康复的牛在一段时间内成为携带者,构成将疾病传播给整个牛群的威胁。诊断带菌牛对预防巴贝斯虫病暴发很重要。因此,本研究的目的是建立一种利用巢式PCR (nPCR)快速检测牛巴贝斯虫病的方法。研究方法:因此,从代表斯里兰卡两个生物气候带的疑似携带牛身上采集了30份血液样本和蜱虫。采用显微法和巢式nPCR法对血样进行分析,检测牛巴贝斯虫病。进一步用显微镜对蜱进行形态鉴定,用nPCR对巴贝斯虫病进行分析。结果:光镜下巴贝虫感染阳性率为47% (14/30),nPCR检测阳性率为90%(27/30)。这表明,43%(13/30)通过常规光镜诊断显示为健康的动物实际上是对健康畜群构成重大威胁的携带者。此外,根据nPCR结果,22%(6/27)的血液样本仅为牛巴贝虫感染阳性,11%(3/27)的血液样本仅为双巴贝虫感染阳性,67%(18/27)的血液样本为两种寄生虫混合感染阳性。两区分离到的优势蜱媒均为微头蜱。检出蜱中有21%(5/24)感染巴贝斯虫。研究局限:由于两个气候带所有站点的样本量不均匀且较小,因此无法对数据进行统计显著性分析。独创性/价值:然而,本研究的结果表明,与传统的显微镜分析相比,nPCR提供了一种灵敏的筛选方法来检测牛巴贝斯虫病。
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引用次数: 0
Factors affecting Adoption of Maize Production Technologies: A study in Ghana 影响玉米生产技术采用的因素:在加纳的研究
IF 0.6 Pub Date : 2018-01-01 DOI: 10.4038/JAS.V13I1.8303
C. Wongnaa, D. Awunyo-Vitor, J. A. Bakang
Purpose: The huge gap between actual and achievable yields in Ghana’s maize production threatens Ghana’s household food security. Poor adoption of improved maize production technologies is often cited as the major cause of the low yields. This study examined the factors influencing adoption of improved production technologies by maize farmers in order to highlight the constraints and opportunities for improving adoption. Research Method: The data used were obtained through a cross-sectional survey of 576 maize farmers in Ghana using the structured questionnaire. Descriptive statistics and the multinomial logit model were the methods of analysis employed. Findings: The results showed that adoption of production technologies is influenced by age, educational level, initial capital outlay, agricultural extension contact, group membership, availability of ready maize market, access to credit, experience in maize farming, land fragmentation and previous year’s price of maize. For adoption of maize production technologies to be improved, technology dissemination programmes should target to  literate farmers and  farmers should be encouraged to join farmer groups, stakeholders should support maize farmers with credit, maize farmers should be provided with ready market and younger farmers should be encouraged to consider maize production as a business. Research Limitations: The study focused on whether or not in general, maize farmers used production technologies. It presents limited information on specific technologies in different agro-ecological zones. Originality/Value: This study provides insights into why especially some Ghanaian maize farmers adopt or do not adopt certain technological packages promoted by the Council for Scientific and Industrial Research (CSIR) and the Ministry of Food and Agriculture (MOFA) of Ghana.
目的:加纳玉米生产的实际产量与可实现产量之间的巨大差距威胁着加纳的家庭粮食安全。改良的玉米生产技术采用不当通常被认为是低产量的主要原因。本研究考察了影响玉米农民采用改良生产技术的因素,以突出改进采用的制约因素和机会。研究方法:使用的数据是通过对加纳576名玉米农民使用结构化问卷进行横断面调查获得的。采用描述性统计和多项logit模型进行分析。结果表明:年龄、受教育程度、初始资本支出、农业推广接触、群体成员、玉米市场可用性、信贷可及性、玉米种植经验、土地破碎化程度和上一年玉米价格对生产技术的采用有影响。为了改进玉米生产技术的采用,技术传播计划应该以有文化的农民为目标,鼓励农民加入农民团体,利益相关者应该为玉米农民提供信贷支持,应该为玉米农民提供现成的市场,应该鼓励年轻的农民将玉米生产视为一项业务。研究局限:该研究关注的是玉米种植者是否普遍使用生产技术。它提供了有限的关于不同农业生态区具体技术的信息。原创性/价值:本研究提供了见解,特别是为什么一些加纳玉米农民采用或不采用由加纳科学和工业研究委员会(CSIR)和粮食和农业部(MOFA)推广的某些技术方案。
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引用次数: 13
Selection of Differentially Expressed Genes Using the Transcriptome Analysis of Ripening Grape Berries in Response to High Temperature 利用成熟葡萄果实对高温响应的转录组分析选择差异表达基因
IF 0.6 Pub Date : 2018-01-01 DOI: 10.4038/JAS.V13I1.8297
Seon Ae Kim, S. Ahn, H. Yun
Purpose: The transcriptome of ‘Campbell Early’ grape berries exposed to high temperature was analyzed to select useful genes by evaluating their differential expression through sequencing short reads on Solexa platform. Research Method: We assembled 118 million high-quality trimmed reads using Velvet followed by Oases. Findings: Functional categorization of expressed transcripts revealed the conservation of genes involved in various biological processes like responses to chemical (12.7%), responses to abiotic stimulus (11.8%), biosynthesis processes (11.8%), and cellular metabolic processes (10.4%) in grape berries exposed to high temperature. The major up-regulated genes included isocitratelyase, cysteine proteinases superfamily protein, cupin family protein, and glycosyl hydrolase genes, and the major down-regulated genes included flavanone 3-hydroxylase, phenylalanine ammonia lyase, chlorophyll A-B binding family protein, and polygalacturonase inhibiting protein genes in grape berries exposed to high temperature. Among genes related to grape coloration, expressions of chalcone and stilbene synthase, flavanone 3-hydroxylase, leucoanthocyanidin dioxygenase, phenylalanine ammonia lyasegenes were more strongly inhibited in berries kept at 35°C than 25°C. Originality/Value: Transcript sets of grape berries generated in this study will provide a resource for gene discovery and development of functional molecular markers to be used in elucidating the mechanism of tolerance to high temperature.
目的:在Solexa平台上对Campbell Early葡萄果实在高温下的转录组进行分析,通过短读测序评估其差异表达,筛选有用基因。研究方法:我们使用Velvet和oasis收集了1.18亿个高质量的修剪reads。结果:表达转录物的功能分类揭示了高温下葡萄果实中涉及多种生物过程的基因的保存,如化学反应(12.7%)、非生物刺激反应(11.8%)、生物合成过程(11.8%)和细胞代谢过程(10.4%)。高温下葡萄果实中上调的基因主要有异柠檬酸酶、半胱氨酸蛋白酶超家族蛋白、库平家族蛋白和糖基水解酶基因,下调的基因主要有黄酮3-羟化酶、苯丙氨酸解氨酶、叶绿素A-B结合家族蛋白和多半乳糖醛酸酶抑制蛋白基因。在与葡萄变色相关的基因中,查尔酮和二苯乙烯合成酶、黄酮3-羟化酶、浅色花青素双加氧酶、苯丙氨酸氨溶酶的表达在35℃的贮藏条件下比在25℃的贮藏条件下受到更强烈的抑制。独创性/价值:本研究生成的葡萄果实转录集将为基因发现和功能分子标记的开发提供资源,用于阐明葡萄耐高温机制。
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引用次数: 3
期刊
Journal of Agricultural Sciences
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